Fraternal Order of Eagles

Charity Fund Department

1623 Gateway Circle S

Grove City, OH 43123

Universal Grant Request Form

Check One:

______ Max Baer Heart Fund ______Robert W. Hansen Diabetes Fund _____ Golden Age Eagle Fund

____ Drug Awareness** ______ D.D. Dunlap Kidney Fund _____ Parkinson’s Fund

(matched up to $500)

____C.P.R. Fund ($1,000) ______ Jimmy Durante Children’s Fund _____ Alzheimer’s & Neurological Fund

______ Art Ehrmann Cancer Fund _____ Child Abuse Prevention _____ Lew Reed Spinal Cord Fund

______ Muscular Dystrophy Fund _____ Children’s Aids _____ H.O.M.E./Earmarked**

□ Regional Grant □ State Grant □ International Grant □ Local Aerie/Aux.

__________________________________________________________________

Project Title: _______________________________________________________________________________________

□ Research □ Education □ Equipment/Supplies

IRS Status: 501(c)_____ IRS Tax Exempt Number - Grant Amount Requested$_____________

Recipients Name: _______________________________________________________________________________________

Department/Site: ________________________________________________________________________________________

Address: ________________________________________________________ City:__________________________________

State/Prov._____________Zip: ________________Contact:__________________________Phone: ______________________

Check Payable To: _______________________________________________________________________________________

Request Submitted By: Aerie/Auxiliary Name: _______________________________________ # _________ Date__________

Presentation Date/Date Check needed by:_________________________________________

Date Approved By State/Provincial Executive Board: ____/_____/_______ Approved Grant Amount: $ __________________

State/Provincial President: ________________________________________ Date: ____/_____/________

State/Provincial Secretary: ________________________________________ Date: ____/_____/________ State/Prov. _______

Local Secretary: ________________________________________________ Date: ____/_____/________

Approved

Local President: ________________________________________________ Date: ____/_____/________ BOGT Misc

Request Form#

*H.O.M.E. and EARMARKED GRANTS DO NOT NEED STATE APPROVAL*

Checklist for: □ Grant Form Completed in Full □ IRS Determination Letter or other Document attesting to

Submission □ Grant Form Signed and Dated nonprofit status is enclosed

□ Verification that Per Capita is Paid □ Project resume on recipient letterhead detailing use of

□ **Contribution enclosed funds and affirming no administrative use of funds

Fraternal Order of Eagles

Proposal for President’s Grant - 2017-2018 State of Florida

Florida Hospital Cancer Institute - Translational Research Core Laboratory (FHCI-TRC)

Request for Equipment for Circulating Tumor Cell Studies

In the fight to stop cancer, most treatments, including surgery, chemotherapy, and radiation therapy, are currently aimed at destroying cells in the main tumor and when they are detectable at new ‘metastatic’ sites. These treatments often must be used at levels that can be hard on the patient’s normal tissues promoting toxic effects such as hair loss, digestive problems, bone loss, and increased susceptibility to infection. However, cells that escape tumors and enter the blood or lymph circulation are most likely the worst players in cancer, evading such treatments and until recently, the hardest to see and track. This is unfortunate because these circulating cells are the cancer at its most vulnerable, lowest numbers which could present a better target for more specific, lower level treatments and could lead to a better outcome for the patient. The lower level of treatments would mean less adverse side effects for the patient and more effective, less expensive therapies.

Circulating tumor cells (CTC) can now be identified and counted using high speed cell sorting instruments. Once collected, CTC can be characterized by sensitive genetic, metabolic, and molecular tests to pinpoint the best sort of treatment to defeat them. Such treatments can be used to create personalized treatment plans designed to lessen toxic side effects and maximize tumor cell elimination. Through the generosity of donors to the Florida Hospital Foundation, we obtained a high-speed fluorescence activated cell sorter (FACS) instrument and developed research methods to isolate and study circulating tumor cells in small blood samples from patient volunteers with lung, multiple myeloma, prostate, leukemia, lymphoma, breast, and pancreatic cancers. Our researchers have also developed molecular and cellular culture methods to understand the character of these cells, finding both what these cancer types have in common as well as what makes each patient’s cancer unique.

Support for research equipment will improve FHCI’s capabilities to detect, examine, and use circulating tumor cells as a tool to characterize each patient’s cancer. Our hope is to personalize and specifically target their treatment to launch the best attack on their cancer while achieving the best results in protecting their health and quality of life. Our first aim is to expand the capacity and adapt our current success with pancreatic cancer circulating tumor cell (CTC) studies using our high speed fluorescence activated cell sorter (FACS) instrument to other cancers by the addition of an ultra-violet laser and detection filters, training of more researchers on the instrument, and with reagents and supplies needed to make the FACS instrument capable of detecting circulating tumor cells with unique biomarkers in multiple types of cancers including pancreatic, breast, lymphomas, leukemia, lung, and prostate cancers.

The FACS instrument is currently equipped with 2 lasers’ detection capacity; however, the capacity can be expanded with the purchase of an ultra-violet laser and additional detection filters. The expansion of the FACS instrument capacities would allow for more advanced studies to discover new cancer-unique biomarkers in more types of cancers; and the ability to look for cancer stem cells, thought to be the well-spring perpetuating cancer’s aggressive re-growth and resistance to treatments over time.

In addition, the Research Laboratory has recently received access to a high sensitivity digital DNA polymerase chain reaction (PCR) Analyzer to expand its ability to detect cancer ‘Cell-Free’ DNA in blood. This capability allows for the earliest diagnosis of cancer and its recurrence.

Funds for training additional researchers to use these instruments, supplies for adapting the CTC work to multiple cancer types, and reagents to expand the depth of our search for new molecular targets and ways to use them to identify and treat tumors would greatly help advance the chances of success for these projects.

We respectfully request the support of the Fraternal Order of Eagles toward the expansion of our CTC multi-cancer research, with equipment upgrades and/or materials and training support that will expand the capabilities of the FHCI-TRC to discover new cancer cell markers in multiple cancer types which will hopefully lead to new ways to defeat these aggressive disease cells.

It is the goal of the FHCI-TRC to understand what makes each patient’s cancer both similar to other cancers, and at the same time, unique to that individual so that these findings can be translated into clinically useful tools and treatments to give every patient the best chance of surviving and thriving after their very personal fight with cancer. We are honored to be selected as the beneficiary of Fraternal Order of Eagles support.