perhitungan analisa ganang penelitian baru

KADAR BETA KAROTEN EKSTRAK ANTIOKSIDAN KULIT KOPI ARABIKA

ANALISA BETA KAROTEN sampel langsung analisaBLANKO Ulangan ABS BM beta karotFP

0.781 1 0.681 536.8726 52 0.676 536.8726 53 0.688 536.8726 54 0.695 536.8726 55 0.701 536.8726 56 0.711 536.8726 5

RATA -RATA

volume sampel (ml)x10-3 [L]0.0020.0020.0020.0020.0020.002

ANALISA BETA KATOTEN SAMPEL disimpan dahuluBLANKO Ulangan ABS BM beta karotFP

0.011 1 0.175 536.8726 52 0.23 536.8726 53 0.228 536.8726 54 0.225 536.8726 55 0.225 536.8726 56 0.23 536.8726 5

RATA -RATA

volume sampel (ml)x10-3 [L]0.0020.002 rumus = ABS*BM karoten*FP*1000/2620*berat sampel*10.0020.0020.0020.002

ANALISA BETA KAROTEN sampel langsung analisaBERAT SAMPEL (GR) KADAR BETA KAROTEN (mg/gr) rumus

0.4 1 3.4890.4 2 3.4630.4 3 3.5250.4 4 3.5600.4 5 3.5910.4 6 3.642

3.545

ANALISA BETA KATOTEN SAMPEL disimpan dahuluBERAT SAMPEL (GR) KADAR BETA KAROTEN (mg/gr)

0.4 1 0.8960.4 2 1.1780.4 3 1.1680.4 4 1.1530.4 5 1.1530.4 6 1.178

1.1210.11061

= ABS*BM karoten*FP*1000/2620*berat sampel*1

= ABS*BM karoten*FP*1000/2620*berat sampel*1

Beta Karoten

data analisa antosianinph 1 520 700 nm ph 4.5 700 nm 520 nmblanko 0.1 0.052 blanko 0.062 0.078abs 1 0.6 0.352 abs 1 0.249 0.378abs 2 0.5 0.321 abs 2 0.295 0.39abs 3 0.5 0.289 abs 3 0.299 0.395

kadar antosianinabs 1 ABS 7.097026022abs 2 ABS 6.59605948

abs 3 ABS 9.100892193

rata-rata 7.597992565

sampel langsung analisaBLANKO ABS PH 1 (520 nmBLANKO PH 1 (700 nmBLANKO PH 4.5 (520 nmBLANKO PH 4.5 (700 nm)BM FP e FAKTOR

0.073 1 0.566 0.052 0.352 0.078 0.378 0.062 0.249 449.2 5 26900 10002 0.495 0.321 0.39 0.295 449.2 5 26900 10003 0.494 0.289 0.395 0.299 449.2 5 26900 1000

RATA-RATA

w (gr) volume sampel (ml)x10-3 [L]0.4 0.0020.4 0.002

0.4 0.002

rumus = A(A520nm-A700nm)-(A520nm-A700nm)*BM*FP*1000/e*1*w(gr)

DATA ANALISA ANTOSIANIN EKSTRAK KULIT BUAH KOPI BARUph 1 520 700 nm ph 4.5 520 nm 700 nmblanko 0.1 0.052 blanko 0.078 0.062abs 1 0.8 0.582 abs 1 0.499 0.361abs 2 0.8 0.596 abs 2 0.487 0.389abs 3 0.7 0.515 abs 3 0.459 0.333

sampel disimpan duluBLANKO ABS PH 1 (520 nmBLANKO PH 1 (700 nmBLANKO PH 4.5 (520 nmBLANKO PH 4.5 (700 nm)BM FP e FAKTOR

0.073 1 0.788 0.052 0.582 0.078 0.499 0.062 0.361 449.2 5 26900 10002 0.76 0.596 0.487 0.389 449.2 5 26900 10003 0.701 0.515 0.459 0.333 449.2 5 26900 1000

RATA-RATA

w (gr) volume sampel (ml)x10-3 [L]0.4 0.0020.4 0.002

0.4 0.002

rumus = A(A520nm-A700nm)-(A520nm-A700nm)*BM*FP*1000/e*1*w(gr)

sampel langsung analisaL kadar antosianin (mg/gr)1 0.0351 0.0331 0.046

0.0379899628252788

sampel disimpan duluL kadar antosianin (mg/gr)1 0.0281 0.0281 0.025

0.02699653035935560.00174

KADAR VITAMIN C EKSTRAK ANTIOKSIDAN KULIT KOPI ARABIKAsampel langsung analisa

data analisa vitamin c

volume N (MOLA FP W(gr)asam askorbat (mg)akhir 1 39.5 0.01 10 15 0.88akhir 2 39 0.01 10 15 0.88akhir 3 38.5 0.01 10 15 0.88awal 50rata-rata

rumus = ml Titrasi*N*0,88mg*FP*100/gr bahanfp 50/5=10

sampel disimpan dahuludata analisa vitamin c

volume N (MOLA FP W(gr)asam askorbat (mg)akhir 1 41 0.01 10 15 0.88akhir 2 40.5 0.01 10 15 0.88akhir 3 40 0.01 10 15 0.88awal 50rata-rata


kadar vitamin c (mg/gr)23.1733333333333

22.8822.5866666666667

22.88


kadar vitamin c (mg/gr)24.0533333333333

23.7623.4666666666667

23.760.2933

KADAR POLIFENOLO EKSTRAK ANTIOKSIDAN KULIT KOPI ARABIKA0.0545

sampel langsung analisaAnalisa polifenol Sampel ABS Perhitungan

Blanko kosentrasi larutan indABS (y)Blanko kosentrasiulangan 1 (yulangan 2 (ynilai a nilai b nilai X indunilai X10.061 1 ml 0.057 0.056 1 ml 0.826 0.754 0.0032 0.0545 0.78 241.09

2 ml 0.062 2 ml 0.831 0.833 0.0032 0.0545 2.34 242.663 ml 0.064 3 ml 0.856 0.843 0.0032 0.0545 2.97 250.474 ml 0.067 4 ml 0.875 0.852 0.0032 0.0545 3.91 256.41

rata-rata 0.0625 0.847 0.8205 0.0032 0.0545 2.5 247.65625

rumus kadar polifenol (mg/gr)y = 0.0032x + 0.0545 x1 x2 v(ml) w(gr) fp

x= y-b/a 1205.47 1092.97 1 0.2 1kadar polifenol = x*fp*v(ml)/w(gr) 1213.28 1216.41 1 0.2 1

1252.34 1232.03 1 0.2 11282.03 1246.09 1 0.2 1

data analisa polifenol dengan sampel disimpan dulu

Analisa polifenol Sampel ABS PerhitunganBlanko kosentrasi larutan indABS (y)Blanko kosentrasiulangan 1 (yulangan 2 (ynilai a nilai b nilai X indunilai X10.061 1 ml 0.054 0.056 1 ml 0.684 0.714 0.0025 0.0515 1 253

2 ml 0.056 2 ml 0.697 0.725 0.0025 0.0515 1.8 258.23 ml 0.06 3 ml 0.734 0.739 0.0025 0.0515 3.4 2734 ml 0.061 4 ml 0.748 0.752 0.0025 0.0515 3.8 278.6

rata-rata 0.0578 0.71575 0.7325 0.0025 0.0515 2.5 265.712.07256

Y=A.X+B 269.0509.3449

kadar polifenol (mg/gr)x1 x2 v(ml) w(gr) fp

1265.00 1325.00 1 0.2 11291.00 1347.00 1 0.2 11365.00 1375.00 1 0.2 11393.00 1401.00 1 0.2 1

Perhitungannilai X2

218.59243.28246.41249.22239.375

1 ml 2 ml 3 ml 4 ml0.061

0.0520.0540.0560.0580.06

0.0620.0640.0660.068

0.057

0.0620.064

0.067f(x) = 0.0032 x + 0.0545

grafik kosentrasi larutan induk dengan ABS

ABS (y)Linear (ABS (y))Linear (ABS (y))

kosentrasi

ABS

1 ml 2 ml 3 ml 4 ml0.056

0.65

0.7

0.75

0.8

0.85

0.9

0.826 0.8310.856

0.875

0.754

0.833 0.843 0.852

grafik kosentrasi sampel dengan ABS

ulangan 1 (y)Linear (ulangan 1 (y))ulangan 2 (y)

kosentrasi

ABS

Perhitungannilai X2

265269.4

275280.2272.4

6.61715

1 ml 2 ml 3 ml 4 ml0.061

0.0520.0540.0560.0580.06

0.0620.0640.0660.068

0.057

0.0620.064

0.067f(x) = 0.0032 x + 0.0545

grafik kosentrasi larutan induk dengan ABS

ABS (y)Linear (ABS (y))Linear (ABS (y))

kosentrasi

ABS

1 ml 2 ml 3 ml 4 ml0.061

0.050.0550.060.065

0.0540.0560.06 0.061

f(x) = 0.0025 x + 0.0515

grafik kosentrasi larutan induk dengan

ABS Column CLinear (Column C)Linear (Column C)

Kosentrasi

ABS

1 ml 2 ml 3 ml 4 ml0.056

0.640.660.680.7

0.720.740.76

0.6840.697

0.7340.748

0.7140.725

0.7390.752

grafik kosentrasi sampel dengan ABS

ulangan 1 (y)ulangan 2 (y)Linear (ulangan 2 (y))

Kosentrasi

ABS

1 ml 2 ml 3 ml 4 ml0.061

0.050.0550.060.065

0.0540.0560.06 0.061

f(x) = 0.0025 x + 0.0515

grafik kosentrasi larutan induk dengan

ABS Column CLinear (Column C)Linear (Column C)

Kosentrasi

ABS

analisa pengukuran ph ekstrak pekat kulit buah kopi arabikasampel lamaulangan hasil

1 2.82 2.843 2.83

rata-rata2.823333

sampel baruulangan hasil

1 3.82 3.963 3.98

rata-rata3.9133330.0987

analisa pengukuran aktifitas antioksidan ekstrak pekat kulit buah kopi arabika

SAMPEL LANGSUNG ANALISA

blanko Ulangan nilai absorbansi0.564 1 0.4450.564 2 0.4820.564 3 0.4410.564 4 0.4520.564 5 0.456

SAMPEL DISIMPAN DAHULU

blanko Ulangan nilai absorbansi0.868 1 0.3650.868 2 0.360.868 3 0.3290.868 4 0.3320.868 5 0.339

1 2 3 4 5 60

0.1

0.2

0.3

0.4

0.5

0.60.564 0.564 0.564 0.564 0.564

0.4450.482

0.441 0.452 0.456

21.10%14.54%

21.81% 19.86% 19.15%

grafik hub nilai abs, efektifitas penghambatan dan blanko

blankonilai absorbansiefektifitas penghambatan (DPPH)

1 2 3 4 5 60

0.10.20.30.40.50.60.70.80.91

0.868 0.868 0.868 0.868 0.868

0.365 0.36 0.329 0.332 0.339

57.95% 58.53% 62.10% 61.75% 60.94% blankonilai absorbansiefektifitas penghambatan (DPPH)

analisa pengukuran aktifitas antioksidan ekstrak pekat kulit buah kopi arabika

efektifitas penghambatan (DPPH)21.10% rumus = (ABS blanko-ABS sampel)*100%/ABS blanko14.54%21.81%19.86%19.15%

efektifitas penghambatan (DPPH)57.95%58.53%62.10%61.75%60.94%60.25%

1 2 3 4 5 60

0.1

0.2

0.3

0.4

0.5

0.60.564 0.564 0.564 0.564 0.564

0.4450.482

0.441 0.452 0.456

21.10%14.54%

21.81% 19.86% 19.15%

grafik hub nilai abs, efektifitas penghambatan dan blanko

blankonilai absorbansiefektifitas penghambatan (DPPH)

0.43 0.44 0.45 0.46 0.47 0.48 0.490%

5%

10%

15%

20%

25%

21.10%

14.54%

21.81%19.86%19.15%f(x) = − 1.77304964539007 x + 1

efektifitas penghambatan (DPPH)

efektifitas penghambatan (DPPH)Linear (efektifitas penghambatan (DPPH))

ABS

prosentase

0.0190

1 2 3 4 5 60

0.10.20.30.40.50.60.70.80.91

0.868 0.868 0.868 0.868 0.868

0.365 0.36 0.329 0.332 0.339

57.95% 58.53% 62.10% 61.75% 60.94% blankonilai absorbansiefektifitas penghambatan (DPPH)

0.32 0.33 0.34 0.35 0.36 0.3754.00%

56.00%

58.00%

60.00%

62.00%

64.00%

57.95%58.53%

62.10%61.75%60.94%f(x) = − 1.15207373271889 x + 1



ABS

prosentase

= (ABS blanko-ABS sampel)*100%/ABS blanko

0.43 0.44 0.45 0.46 0.47 0.48 0.490%

5%

10%

15%

20%

25%

21.10%

14.54%

21.81%19.86%19.15%f(x) = − 1.77304964539007 x + 1



ABS

prosentase

0.32 0.33 0.34 0.35 0.36 0.3754.00%

56.00%

58.00%

60.00%

62.00%

64.00%

57.95%58.53%

62.10%61.75%60.94%f(x) = − 1.15207373271889 x + 1



ABS

prosentase

analisa pengukuran warna ekstrak pekat kulit buah kopi arabika

sampel langsung analisaANALISA WARNA Hasil Pengukuran Warna

posisi sudutdl da db L a* b* c*depan -23.6 -3.6 6.1 70.75 -9.35 12.61 15.69824kanan -24 -1.8 5.2 70.35 -7.55 11.71 13.93293kiri -22.8 -5.7 5.6 71.55 -11.45 12.11 16.66597belakang -23.5 -2.4 5 70.85 -8.15 11.51 14.10328bawah -24 -0.8 6.6 70.35 -6.55 13.11 14.65519rata-rata 70.77 -8.61 12.21 15.01112

data analisa pengukuran warna dengan sampel ekstrak baru ( ulangan 1)ANALISA WARNA Hasil Pengukuran Warna

posisi sudutdl da db L a* b* c*depan -29 -1.8 2.3 65.35 -7.55 8.81 11.60253kanan -31.1 -2.9 3.6 63.25 -8.65 10.11 13.30543kiri -30.9 -1.3 3.5 63.45 -7.05 10.01 12.24347belakang -29.8 -2.4 6.1 64.55 -8.15 12.61 15.01448bawah -29.5 -1.3 3.3 64.85 -7.05 9.81 12.0805rata-rata 64.29 -7.69 10.27 12.84928

data analisa pengukuran warna dengan sampel ekstrak baru ( ulangan 2)ANALISA WARNA Hasil Pengukuran Warna

posisi sudutdl da db L a* b* c*depan -30.9 -1.1 3.5 63.45 -6.85 10.01 12.12941

kanan -30.8 -1.1 3.4 63.55 -6.85 9.91 12.04702kiri -31 -1.7 2.9 63.35 -7.45 9.41 12.00211belakang -31.1 -1 3.7 63.25 -6.75 10.21 12.23955bawah -29.3 -1.1 2.6 65.05 -6.85 9.11 11.39801rata-rata 63.73 -6.95 9.73 11.96322

sampel lansung di analisa Hasil Pengukuran Warna ANALISA WARNA

H standard L standard a standarb b*/a* posisi su-53.44 94.35 -5.75 6.51 -1.35 depan -57.19 94.35 -5.75 6.51 -1.55 kanan-46.60 94.35 -5.75 6.51 -1.06 kiri-54.70 94.35 -5.75 6.51 -1.41 belakang -63.45 94.35 -5.75 6.51 -2.00 bawah

-55.0775 rata-rata

data analisa pengukuran warna dengan sampel disimpan dahulu ( ulangan 1)AHasil Pengukuran Warna ANALISA WARNA

H standard L standard a standarb b*/a* posisi su-49.40 94.35 -5.75 6.51 -1.17 depan -49.45 94.35 -5.75 6.51 -1.17 kanan-54.84 94.35 -5.75 6.51 -1.42 kiri-57.12 94.35 -5.75 6.51 -1.55 belakang -54.30 94.35 -5.75 6.51 -1.39 bawah

-53.0238 rata-rata

data analisa pengukuran warna dengan sampel disimpan dahulu ( ulangan 2) BHasil Pengukuran Warna ANALISA WARNA

H standard L standard a standarb b*/a* posisi su-55.62 94.35 -5.75 6.51 -1.46 depan

-55.35 94.35 -5.75 6.51 -1.45 kanan-51.63 94.35 -5.75 6.51 -1.26 kiri-56.53 94.35 -5.75 6.51 -1.51 belakang -53.06 94.35 -5.75 6.51 -1.33 bawah

-54.4368 rata-rata

sampel lansung di analisa ANALISA WARNA Hasil Pengukuran Warna

dl da db L a* b* c* H-23.6 -3.6 6.1 70.75 2.15 12.61 12.79197 80.32-24 -1.8 5.2 70.35 3.95 11.71 12.35826 71.36

-22.8 -5.7 5.6 71.55 0.05 12.11 12.1101 89.76-23.5 -2.4 5 70.85 3.35 11.51 11.9876 73.77-24 -0.8 6.6 70.35 4.95 13.11 14.01337 69.31

rata-rata 70.77 2.89 12.21 12.65226 76.90688

data analisa pengukuran warna dengan sampel disimpan dahulu ( ulangan 1)AANALISA WARNA Hasil Pengukuran Warna

dl da db L a* b* c* H-29 -1.8 2.3 65.35 3.95 8.81 9.654978 65.85

-31.1 -2.9 3.6 63.25 2.85 10.11 10.50403 74.26-30.9 -1.3 3.5 63.45 4.45 10.01 10.95457 66.03-29.8 -2.4 6.1 64.55 3.35 12.61 13.0474 75.12-29.5 -1.3 3.3 64.85 4.45 9.81 10.77212 65.60

rata-rata 64.29 3.81 10.27 10.98662 69.37244

data analisa pengukuran warna dengan sampel disimpan dahulu ( ulangan 2) BANALISA WARNA Hasil Pengukuran Warna

dl da db L a* b* c* H-30.9 -1.1 3.5 63.45 4.65 10.01 11.03733 65.08

-30.8 -1.1 3.4 63.55 4.65 9.91 10.94672 64.86-31 -1.7 2.9 63.35 4.05 9.41 10.24454 66.71

-31.1 -1 3.7 63.25 4.75 10.21 11.26084 65.05-29.3 -1.1 2.6 65.05 4.65 9.11 10.22813 62.96

rata-rata 63.73 4.55 9.73 10.74351 64.93388

Hasil Pengukuran Warnastandard L standard a standarb b*/a*

94.35 5.75 6.51 5.8794.35 5.75 6.51 2.9694.35 5.75 6.51 242.2094.35 5.75 6.51 3.4494.35 5.75 6.51 2.65


94.35 5.75 6.51 2.2394.35 5.75 6.51 3.5594.35 5.75 6.51 2.2594.35 5.75 6.51 3.7694.35 5.75 6.51 2.20


94.35 5.75 6.51 2.15

94.35 5.75 6.51 2.1394.35 5.75 6.51 2.3294.35 5.75 6.51 2.1594.35 5.75 6.51 1.96

1:5 (10%)BLANKO Ulangan ABS BM beta karotenFP BERAT SAMPEL (GR) KADAR BETA KAROTEN(mg/gr)

0.062 1 0.356 536.8726 50 0.5 12 0.323 536.8726 50 0.5 23 0.364 536.8726 50 0.5 3

RATA -RATA


0.062 1 0.399 536.8726 50 0.5 12 0.407 536.8726 50 0.5 23 0.397 536.8726 50 0.5 3

RATA -RATA


0.062 1 0.432 536.8726 50 0.5 12 0.453 536.8726 50 0.5 23 0.497 536.8726 50 0.5 3

RATA -RATA

1:5 kontrolBLANKO Ulangan ABS BM beta karotenFP BERAT SAMPEL (GR) KADAR BETA KAROTEN(mg/gr)

0.062 1 0.238 536.8726 50 0.5 12 0.274 536.8726 50 0.5 23 0.272 536.8726 50 0.5 3

RATA -RATA

1:5 (10%) fp (5/0.1)= 50KADAR BETA KAROTEN(mg/gr)

0.7290.662 volume pengambilan (1:4 dan 1:5) ml x10-3 [L]0.746 0.00010.712

1:5 (20%)KADAR BETA KAROTEN(mg/gr)

0.8180.8340.8140.822


0.8850.9281.0180.944

1:5 kontrolKADAR BETA KAROTEN(mg/gr)

0.4880.5610.5570.536


0.062 1 0.273 536.8726 50 0.5 12 0.291 536.8726 50 0.5 23 0.299 536.8726 50 0.5 3

RATA -RATA


0.062 1 0.326 536.8726 50 0.5 12 0.345 536.8726 50 0.5 23 0.36 536.8726 50 0.5 3

RATA -RATA


0.062 1 0.489 536.8726 50 0.5 12 0.495 536.8726 50 0.5 23 0.475 536.8726 50 0.5 3

RATA -RATA

1:4 (kontrolBLANKO Ulangan ABS BM beta karotenFP BERAT SAMPEL (GR) KADAR BETA KAROTEN(mg/gr)

0.062 1 0.81 536.8726 50 0.5 12 0.826 536.8726 50 0.5 23 0.812 536.8726 50 0.5 3

RATA -RATA


0.5590.5960.6130.589


0.6680.7070.7380.704


1.0021.0140.9730.997

1:4 (kontrolKADAR BETA KAROTEN(mg/gr)

1.6601.6931.6641.672

dpphsampel 1:5 (10%)

blanko Ulangan nilai absorbansi0.845 1 0.1080.845 2 0.1110.845 3 0.108

sampel 1:5 (20%)


sampel 1:5 (30%)


sampel 1:5 (kontrol)


sampel 1:5 (10%) sampel 1:4 (10%)

efektifitas penghambatan (DPPH) blanko87.22% 0.84586.86% 0.84587.22% 0.84587.10%0.002

sampel 1:5 (20%) sampel 1:4 (20%)


sampel 1:5 (30%) sampel 1:4 (30%)


sampel 1:5 (kontrol) sampel 1:4 (kontrol)


sampel 1:4 (10%)

Ulangan nilai absorbansi efektifitas penghambatan (DPPH)1 0.109 87.10%2 0.107 87.34%3 0.108 87.22%

87.22%0.001

sampel 1:4 (20%)


85.40%0.002

sampel 1:4 (30%)


83.87%0.001

sampel 1:4 (kontrol)


81.58%0.002

sampel 1:5

kosentrasivolume akhir N (MOLAFP W(gr)asam askorbat (mg)10% 47 0.01 1 0.5 0.8820% 46.8 0.01 1 0.5 0.8830% 46.5 0.01 1 0.5 0.88

kontrol 46 0.01 1 0.5 0.88rata-rata 46.575

sampel 1:4

kosentrasivolume akhir N (MOLAFP W(gr)asam askorbat (mg)10% 46.3 0.01 1 0.5 0.8820% 46.2 0.01 1 0.5 0.8830% 45.8 0.01 1 0.5 0.88

kontrol 42.3 0.01 1 0.5 0.88rata-rata 45.15

sampel 1:5 sampel 1:5 a

kadar vitamin c (mg/gr) kosentrasivolume akhir N (MOLA82.72 10% 46.5 0.0182.37 20% 46 0.0181.84 30% 45.7 0.0180.96 kontrol 42.3 0.01

82.3093333333333 rata-rata 45.125

sampel 1:5 81.69

sampel 1:4 sampel 1:4 a

kadar vitamin c (mg/gr) kosentrasivolume akhir N (MOLA81.49 10% 45.9 0.0181.31 20% 45.2 0.0180.61 30% 44.8 0.0174.45 kontrol 42 0.0181.136 rata-rata 44.475

sampel 1:4 80.432

sampel 1:5 a

FP W(gr)asam askorbat (mg)kadar vitamin c (mg/gr)1 0.5 0.88 81.84 10 82.28 0.6222541 0.5 0.88 80.96 20 81.66 0.99560631 0.5 0.88 80.43 30 81.14 0.99560631 0.5 0.88 74.448 kontrol 77.70 4.6046794

81.0773333333333

sampel 1:4 a

FP W(gr)asam askorbat (mg)kadar vitamin c (mg/gr)1 0.5 0.88 80.78 10 81.14 0.49780321 0.5 0.88 79.55 20 80.43 1.24450791 0.5 0.88 78.85 30 79.73 1.24450791 0.5 0.88 73.92 kontrol 74.18 0.3733524

79.728

sampel 1:5 (10%)Analisa polifenol Sampel

BLANKO induk kosentrasi larutan indABS (Y) Blanko Sampel0.065 1 ml 0.054 0.0560.065 2 ml 0.0560.065 3 ml 0.060.065 4 ml 0.061

rata-rata 0.05775

rumusy = 0.0025x + 0.0515

x= y-b/akadar polifenol = x*fp*v(ml)/w(gr)


BLANKO induk kosentrasi larutan indABS (y) Blanko Sampel0.065 1 ml 0.054 0.0560.065 2 ml 0.0560.065 3 ml 0.060.065 4 ml 0.061 kadar polifenol (mg/gr)

rata-rata 0.05775 x1958.00



1 ml 2 ml 3 ml 4 ml0.065 0.065 0.065 0.065

0.05

0.052

0.054

0.056

0.058

0.06

0.062

0.054

0.056

0.060.061f(x) = 0.0025 x + 0.0515

Column CLinear (Column C)

rata-rata 0.05775 x11358.00

sampel 1:5 (kontrol)Analisa polifenol Sampel


rata-rata 0.05775 x11838.00

sampel 1:5 (10%)ABS Perhitungan

ulangan 1 (Yulangan 2 (Y)nilai a nilai b nilai X induk0.194 0.187 0.0025 0.0515 1

kadar polifenol (mg/gr)x1 x2 v(ml) w(gr)

570.00 542.00 0.1 0.5



kadar polifenol (mg/gr)x2 v(ml) w(gr) fp

978.00 0.1 0.5 50



kadar polifenol (mg/gr)

1 ml 2 ml 3 ml 4 ml0.065 0.065 0.065 0.065

0.05

0.052

0.054

0.056

0.058

0.06

0.062

0.054

0.056

0.060.061f(x) = 0.0025 x + 0.0515

Column CLinear (Column C)

x2 v(ml) w(gr) fp1398.00 0.1 0.5 50

sampel 1:5 (kontrol)ABS Perhitungan


kadar polifenol (mg/gr)x2 v(ml) w(gr) fp

1866.00 0.1 0.5 50

sampel 1:5 (10%)Perhitungan

nilai X sampel nilai X sampel 257 54.2

55.6001.97990

kadar polifenol (mg/gr)fp

50

fp=5/0.1: 50w= 0.5 gr


nilai X sampel nilai X sampel 295.8 97.8

96.8001.41421



137.8002.82843

sampel 1:5 (kontrol)Perhitungan


185.2001.97990


BLANKO inkosentrasi larutaABS (y) Blanko Sampel0.065 1 ml 0.054 0.0560.065 2 ml 0.0560.065 3 ml 0.060.065 4 ml 0.061

rata-rata 0.05775



rata-rata 0.05775



rata-rata 0.05775

sampel 1:4 (kontrol)Analisa polifenol Sampel


rata-rata 0.05775

sampel 1:4 (10%)Sampel ABS Perhitungan

Blanko Sampel ulangan 1 (Y)ulangan 2 (Ynilai a nilai bnilai X induk0.056 0.797 0.801 0.0025 0.0515 1

kadar polifenol (mg/gr)x1 x2 v(ml) w(gr) fp2982.00 2998.00 0.1 0.5 50







sampel 1:4 (kontrol)Sampel ABS Perhitungan




nilai X sampel 1nilai X sampel 2298.2 299.8

299.0001.13137



254.0005.93970



199.2001.41421

sampel 1:4 (kontrol)Perhitungan

nilai X sampel 1nilai X sampel 2140.6 143

141.8001.69706

sampel 1:5(10%)BLANKO ABS PH 1 (520BLANKO PH 1 (700 BLANKO PH 4.5 (520BLANKOPH 4.5 (700 )

0.071 1 0.078 0.059 0.065 0.071 0.07 0.059 0.0622 0.081 0.062 0.075 0.061

RATA-RATAw (gr) volume sampel (ml)x10-3 [L]

0.5 0.00010.5 0.0001

sampel 1:5(20%)BLANKO ABS PH 1 (520BLANKO PH 1 (700 BLANKO PH 4.5 (520BLANKOPH 4.5 (700 nm)

0.071 1 0.07 0.059 0.06 0.071 0.069 0.059 0.0622 0.071 0.061 0.07 0.064


0.5 0.00010.5 0.0001

sampel 1:5(30%)BLANKO ABS PH 1 (520BLANKO PH 1 (700 BLANKO PH 4.5 (520BLANKOPH 4.5 (700 nm)

0.071 1 0.072 0.059 0.064 0.071 0.068 0.059 0.0622 0.072 0.065 0.07 0.065


0.5 0.00010.5 0.0001

sampel 1:5(konrol)BLANKO ABS PH 1 (520BLANKO PH 1 (700 BLANKO PH 4.5 (520BLANKOPH 4.5 (700 nm)

0.071 1 0.072 0.059 0.058 0.071 0.069 0.059 0.0612 0.073 0.059 0.071 0.063


0.5 0.00010.5 0.0001

sampel 1:5(10%)BM FP e FAKTORL kadar antosianin (mg/gr)449.2 50 26900 1000 1 0.0008349 fp:5/0,1=50449.2 50 26900 1000 1 0.0008349

0.000.00

sampel 1:5(20%)BM FP e FAKTORL kadar antosianin (mg/gr)449.2 50 26900 1000 1 0.0005010449.2 50 26900 1000 1 0.0006680

0.000.000

sampel 1:5(30%)BM FP e FAKTORL kadar antosianin (mg/gr)449.2 50 26900 1000 1 0.000333977695167284449.2 50 26900 1000 1 0.000333977695167284

0.00

sampel 1:5(konrol)BM FP e FAKTORL kadar antosianin (mg/gr)449.2 50 26900 1000 1 0.00100193308550186449.2 50 26900 1000 1 0.00100193308550186

0.000.00

sampel 1:4(10%)BLANKO ABS PH 1 (520BLANKO PH 1 (700BLANKO PH 4.5 (520BLANKO

0.071 1 0.07 0.059 0.06 0.071 0.067 0.0592 0.071 0.065 0.064


0.5 0.00010.5 0.0001


0.071 1 0.068 0.059 0.06 0.071 0.066 0.0592 0.069 0.064 0.065


0.5 0.00010.5 0.0001


0.071 1 0.069 0.059 0.059 0.071 0.068 0.0592 0.069 0.06 0.067


0.5 0.00010.5 0.0001

sampel 1:4(kontrol)BLANKO ABS PH 1 (520BLANKO PH 1 (700BLANKO PH 4.5 (520BLANKO

0.071 1 0.074 0.059 0.06 0.071 0.068 0.0592 0.077 0.062 0.07


0.5 0.00010.5 0.0001

sampel 1:4(10%)PH 4.5 (700 nm)BM FP e FAKTOL kadar antosianin (mg/gr)

0.061 449.2 50 26900 1000 1 0.0006679553903345730.062 449.2 50 26900 1000 1 0.000667955390334571

0.000.00


0.061 449.2 50 26900 1000 1 0.000500966542750930.063 449.2 50 26900 1000 1 0.00050096654275093

0.00


0.06 449.2 50 26900 1000 1 0.0003339776951672860.06 449.2 50 26900 1000 1 0.000333977695167286

0.00

sampel 1:4(kontrol)PH 4.5 (700 nm)BM FP e FAKTOL kadar antosianin (mg/gr)

0.062 449.2 50 26900 1000 1 0.001335910780669140.063 449.2 50 26900 1000 1 0.00133591078066914

0.000

Ulangan123456

Kadar Air % (wb) = (b – c)/(b – a) x 100 %

1 4

Sampel Berat botol kosong Berat sampel Berat botol + sampel(a gram) (ml) (b gram)

T10G90 11.78 2 13.809T20G80 11.75 2 13.779T30G70 9.838 2 11.867T0G100 9.776 2 11.805

17.757 2 19.58610.156 2 12.185

1 5

Sampel Berat botol kosong Berat sampel Berat botol + sampel(a gram) (g) (b gram)

T10G90 10.149 1 11.149T20G80 9.834 1 10.834T30G70 9.781 1 10.781T0G100 11.748 1 12.748

KA (%)Berat botol+sampel(c 1 gram)11.808 0.986211.776 0.98729.865 0.98679.807 0.984717.587 1.092910.186 0.9852 0.986

0.0437

KA (%)Berat botol+sampel(c gram)11.09 0.05910.834 010.708 0.07312.645 0.103

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Dioven 100˚C

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