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WinFLOW Software V4.2

Operators Manual

151 Graham Road P.O. Box 9010 College Station, Texas 77842-9010

Telephone (979) 690-1711 FAX (979) 690-0440 www.oico.com [email protected]

Flow SolutionIVSystem

Flow Solution3100 System


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ii

Notice

The information contained in this document may be revised without notice.

OI Analytical shall not be liable for errors contained herein or for incidental or

consequential damages in connection with the furnishing, performance, or use of this

material.

No part of this document may be reproduced, photocopied, or translated to another

language without the prior written consent of OI Analytical.

Rev. 2.3 July 2007

OI Analytical Part Number A001965

Printed in the U.S.A.

Publication 134800707

Copyright 20052007 OI Analytical

Adobe and Acrobat Reader are registered trademarks of Adobe Systems, Inc.

Windows is a registered trademark of Microsoft Corporation.CNSolution, Flow Solution, and WinFLOW are trademarks or registered trademarks of OI Analytical.


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iii

Limited Warranty

OI Analytical warrants each OI Analytical manufactured product against defects in

materials and workmanship under normal use and service for a period of one year.

Equipment installed by OI Analytical is warranted from the installation date; all other

equipment is warranted from the ship date. If purchaser schedules or delays installation

more than 90 days after delivery, then the warranty period starts on the 91st day from

date of shipment. This warranty extends only to the original purchaser. OI Analyticalwill, at its option, repair or replace equipment that proves to be defective during the

warranty period, provided the equipment is returned to OI Analytical at the expense of

the purchaser.

Consumables, expendables, and parts are warranted for 30 days and are not covered

under extended warranties or service contracts.

OI Analytical warrants for a period of one year from the date of delivery: (i) the

Software, when installed and used with an OI Analytical recommended hardware

configuration, will perform in substantial conformance with the documentation

supplied with the Software; and (ii) the physical media on which the Software is

furnished will be free from defects in materials and workmanship under normal use.

This warranty shall not apply to defects originating from, but not limited to, the

following:

Improper maintenance or operation by the purchaser;

Purchaser supplied accessories or consumables;

Modification or misuse by the purchaser;

Operation outside the products environmental and electrical specifications;

Software, interfacing, parts, or supplies not supplied by OI Analytical;

A computer not meeting the minimum specifications recommended by OI

Analytical;

Improper or inadequate site preparation;

Purchaser induced contamination or leaks.

THE FOREGOING WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES,

EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO ANYWARRANTY OF MERCHANTABILITY, FITNESS, OR ADEQUACY FOR ANY

PARTICULAR PURPOSE OR USE. OI ANALYTICAL SHALL NOT BE LIABLE

FOR ANY SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES,

WHETHER IN CONTRACT, TORT, OR OTHERWISE.

Any service requests or questions should be directed to the OI Analytical Customer

Support Center at (800) 336-1911 or (979) 690-1711.


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iv

Safety Information

For operator safety, pay special attention to the WARNINGand CAUTIONstatements

throughout the manual.

A WARNINGindicates a condition or possible situation that could result in

physical injury to the operator.

A CAUTIONindicates a condition or possible situation that could damage ordestroy the product or the operators work.

Warnings and precautions in this manual or on the instrument must be followed during

operation, service, and repair of the instrument. Failure to follow these warnings and

precautions violates the safety design standards and intended use of the instrument.

OI Analytical is not liable for the operators failure to comply with warnings and

precautions.


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v

Table of Contents

Notice .............................................................................................................................. ii

Limited Warranty ........................................................................................................ iii

Safety Information ....................................................................................................... iv

Chapter 1: Introduct ion ........................................................1Features .......................................................................................................................... 1

Computer Requirements .............................................................................................. 2

Security Settings for WindowsXP Operating Systems...........................................2

Chapter 2: Instal lat ion ..........................................................4Installing WinFLOW.................................................................................................... 4

Security Settings for WindowsXP Operating Systems ........................................... 5

Chapter 3: Configurat ion .....................................................7Configuring WinFLOW ............................................................................................... 7

Configuring the RA Sampler ....................................................................................... 9

Vial Depths..............................................................................................................10

Dilutor Setup ........................................................................................................... 12

Wash and Diluent ....................................................................................................13

Rack Placement .......................................................................................................14

Rack Editor..............................................................................................................15

Configuring the 3360 Sampler................................................................................... 18

Vial Depths..............................................................................................................19

Dilutor Setup ........................................................................................................... 20

Wash and Diluent ....................................................................................................21

Rack Placement .......................................................................................................21

Peck Probe during Rinse .........................................................................................22

Configuring the 3090 Sampler................................................................................... 22

Vial Depths..............................................................................................................23

Wash and Diluent ....................................................................................................24

Rack Placement .......................................................................................................25

Peck Probe during Rinse .........................................................................................25

Chapter 4: Introduction to WinFLOW ...............................26Starting WinFLOW .................................................................................................... 26

Select View Dialog Box .......................................................................................... 26

Tools Menu................................................................................................................... 27Preferences .............................................................................................................. 28

Configuration...........................................................................................................28

Events Scheduler .....................................................................................................29

Instrument Control...................................................................................................30

Pump Timer.............................................................................................................30

Window Menu ............................................................................................................. 30

About Menu ................................................................................................................. 31


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Shortcut Keys .............................................................................................................. 31

Icon Ribbon.................................................................................................................. 31

System Security ........................................................................................................... 33

WinFLOW Quick Start .............................................................................................. 34

Chapter 5: Method Editor ...................................................36General Description .................................................................................................... 36

Method Editor Icon Ribbon ..................................................................................... 37

Opening a Method ...................................................................................................37

Creating a Method ...................................................................................................38

Saving a Method......................................................................................................38

Flow Control Settings Screen ..................................................................................... 38

Instrument................................................................................................................39

Sampling Mode .......................................................................................................39

CCV/Drift Fail.........................................................................................................41

Vial Types ............................................................................................................... 41

Off-scale (HI) Samples............................................................................................41

Insert Baselines........................................................................................................42

Pump Control...........................................................................................................42Detector/Channel Settings Screen ............................................................................. 43

Channels .................................................................................................................. 43

Name .......................................................................................................................43

Methodology ........................................................................................................... 44

CAS Number ........................................................................................................... 44

Detector Settings .....................................................................................................44

Channel Subtractions...............................................................................................45

Signal Filter and Marking Settings Screen............................................................... 46

Smoothing ............................................................................................................... 47

Baseline Marking.....................................................................................................48

Inverted Chemistry..................................................................................................48

Peak Marking...........................................................................................................48Calibration/Quantitation Settings Screen................................................................. 50

Calibration............................................................................................................... 51

Quantitation............................................................................................................. 51

Flagging...................................................................................................................52

Overlimit Samples...................................................................................................52

Timed Events Editor Screen....................................................................................... 52

B (Basis of Timing).................................................................................................53

Time.........................................................................................................................53

Device......................................................................................................................53

# (Selector) .............................................................................................................. 54

State .........................................................................................................................54

Calibrants Table Editor Screen ................................................................................. 55Calibrants.................................................................................................................55

Independent Calibration Verification ......................................................................56

Continuing Calibration Verification........................................................................56

Spike........................................................................................................................56

Stock Calibrants.......................................................................................................57

Drift Correction .......................................................................................................57

Verifying Method Settings .......................................................................................... 57


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vii

Chapter 6: Sample Table....................................................58Cup Number ............................................................................................................ 59

Sample Names.........................................................................................................60

Sample Type............................................................................................................60

Replicate..................................................................................................................61

Dilution and Weight Factors ...................................................................................61

Vial Type.................................................................................................................61

Comments................................................................................................................61Sample Table Menu Options ...................................................................................... 62

File Menu ................................................................................................................ 62

Edit Menu................................................................................................................ 62

Tools and Window Menus.......................................................................................63

Creating a Sample Table............................................................................................. 63

Synchronization Peak (SYNC)................................................................................63

Carryover Correction (CO)......................................................................................63

Calibrants.................................................................................................................63

Calibrants To Be Diluted.........................................................................................63

Unknowns To Be Diluted........................................................................................64

Stock Solutions........................................................................................................64

Incremental Sensitivity Drift Correction .................................................................65Total Sensitivity Drift Correction............................................................................65

Independent Calibration Verification ......................................................................65

Continuing Calibration Verification........................................................................65

Spiked Samples .......................................................................................................66

Blanks and Baseline Samples..................................................................................66

Autodilution ................................................................................................................. 67

Import and Export Functions .................................................................................... 68

Exporting a Table ....................................................................................................68

Importing a Table ....................................................................................................68

Chapter 7: Data Collection .................................................69Initiating Data Collection ........................................................................................... 69

Monitoring the Baseline.............................................................................................. 72

Starting a Sample Analysis......................................................................................... 73

Peak Plot Screen......................................................................................................73

Calibration Plot Screen............................................................................................73

Calibration Results Screen ...................................................................................... 74

Peak Table Results Screen.......................................................................................75

Adding Samples to a Sample Table.........................................................................75

Modifying a Sample Table During a Run ................................................................. 76

Stopping a Run ............................................................................................................ 76

Operator-Stopped Run.............................................................................................76

WinFLOW-Stopped Run.........................................................................................76


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Chapter 8: Results and Analys is .......................................77Loading a Data File..................................................................................................... 77

Data Processing Tools ................................................................................................. 78

Data Analysis Screen...............................................................................................78

Grid On/Off Icon .....................................................................................................78

Peak Editor Icon ......................................................................................................78

Recalculate Icon ......................................................................................................78

Delete/Reprocess Peaks Icon...................................................................................79Menu Options .......................................................................................................... 80

Peak Plot Screen.......................................................................................................... 82

Magnifying the Peak Plot ........................................................................................ 82

Using the Cursor For Peak Identification................................................................83

Changing a Peak State (Add, Delete, Toggle Baseline Point).................................83

Calibration Plot Screen............................................................................................... 84

Magnifying the Calibration Plot..............................................................................84

Using the Cursor for Calibration Point Identification .............................................84

Removing Outlier Calibration Points ...................................................................... 84

Peak Results Table Screen.......................................................................................... 85

Peak Results Table Fields........................................................................................85

Calibration Results Table Screen............................................................................... 87Calibrant ID.............................................................................................................87

Calibration Coefficients...........................................................................................87

Correlation Coefficient............................................................................................87

Carryover.................................................................................................................87

Drift Correction .......................................................................................................87

Reports ......................................................................................................................... 88

Exporting a Report ..................................................................................................... 89

Chapter 9: Troubleshooting...............................................90Instrument Errors....................................................................................................... 90

WinFLOW Errors....................................................................................................... 91Reporting Errors ......................................................................................................92


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WinFLOW Software Operators Manual: Chapter 1 1

Rev. 2.3

Ch a p t e r 1 Introduction

The WinFLOW software is a Windows-based program designed to offer a high

level of automation when used with any of the OI Analytical Flow Solution

systems,as well as many other automated chemistry analyzers.

Features

Automatic startup and shutdown enhances system productivity.*

Pause option permits run and sample table modification anytime during a run.

Sensitivity drift correction eliminates the need to fully recalibrate after ICV/CCV

failure.

Operator-defined dilution factors add operator flexibility in autodilution mode.*

Sample dilution before the run reduces analysis time.

Automatic working calibration standard preparation from stock solutions reduces

operator workload.*

Manual injection mode permits system operation without an autosampler.

Peak editing improves peak height and area marking.

Post-run peak height, area, and digital signal processing algorithms enhance

analyte detectability.

Mean and RSD values add important QA/QC data to reports.

Options provide customized reporting capabilities.

Complete French, German, and Spanish language support provides enhanced

usability.

*This feature requires specific hardware for full system functionality.

The WinFLOW software package includes the following:

CD-ROM containing the following:

WinFLOW Software

Hardware Operators Manual (one of the following):

WinFLOWSoftware Operators Manual

Flow SolutionIV Operators Manual

Flow SolutionIVOperators Manual

Flow Solution3000 Operators Manual

Flow Solution3100 Operators Manual

CNSolutionFS 3100 Operators Manual

Adobe

Acrobat Reader

WinFLOWsoftware serial number (labeled on the outside of the CD-ROM case)


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Introduction


Rev. 2.3

Computer Requirements

The following table contains minimum and recommended computer requirements for

successfully installing and using the WinFLOWsoftware.

NOTE: Using WinFLOWsoftware requires a working knowledge of the Windows

2000 or WindowsXP Pro operating system.

NOTE: Flow SolutionIII and Flow SolutionIV systems larger than three channels

or Flow Solution3000 systems larger than two channels require the

recommended computer requirements.

NOTE: Power management features (excluding the monitor) can cause shutdowns

and errors during analysis.

Securi ty Settings

for WindowsXPOperatingSystems

When using the WinFLOWsoftware with a WindowsXP Pro operating system, set

permissions to Everyonefor full control of all WinFLOWsoftware features. The

default setting for the WindowsXP Pro operating system is NO users. Therefore,

change permissions to Everyone after installing WinFLOWsoftware on the

computer.

1. Enable Full Control to Everyone by right-clicking Startat the screens lower left

corner.

2. Click Explore.

3. Find the Folder named Flow_4.

Table 1.1. Computer requirements

Parameter Minimum Recommended

Type IBM compatible IBM compatible

Processor speed 1 GHz 3 GHz

Memory 512 MB 1 GB or greater

Free disk space 500 MB 1 GB or greater

Operating system Windows2000 or XP

Pro

WindowsXP Pro

Graphics SVGA XGA

COM port One available RS-232 or

USB-to-RS-232 converter

One available RS-232


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Introduction


Rev. 2.3

NOTE: WinFLOW normally installs in this folder. If the software installed in a

different location, go to that folder.

4. Right-click the Flow_4 folder and select Properties.

5. Under the Securitytab, click the Addbutton.

6. In the Select Users, Computers, or Groups dialog box, type the word Everyone

under the Enter the object names to select sectionand click OK(see Figure 1.1).

Figure 1.1. Permissions dialog box

7. Click the checkbox under Allownext to Full Controlto enable full control.

8. Click Applyand OKto exit the screen.
http://-/?-http://-/?-


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Rev. 2.3

Ch a p t e r 2 Installation

This chapter presents step-by-step procedures for properly installing WinFLOW.

Installing WinFLOW

The WinFLOW CD contains WinFLOW software, the WinFLOW software manual,

and all instrument operation manuals. Install WinFLOW using the following

instructions:

1. From the Windows environment, insert the WinFLOW CD into the available CD-

ROM drive. The WinFLOW Setup Disc screen appears (Figure 2.1).

Figure 2.1. WinFLOW Setup Disc screen

If the WinFLOW Setup Disc screen does not automatically appear after inserting

the CD:

Click the Windows Startbutton.

Select Run.

Typex:\setup,wherexrepresents the letter assigned to the CD-ROM drive.

Click OKto display the WinFLOW Setup Disc screen.

2. Select the appropriate language and click Installto begin the installation process.

Follow the instructions on the screen.

NOTE: Do not use more than eight characters or spaces in the directory names.


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Installation


Rev. 2.3

3. After WinFLOW installs, click OK. The Configure WinFLOW dialog box appears

(Figure 2.2). Proceed to Chapter 3, Configuration on page 7for details on

configuring WinFLOW.

Figure 2.2. Configure WinFLOW dialog box

Security Settings for WindowsXP Operating Systems

When using WinFLOW with a Windows XP operating system, set permissions to

Everyonefor full control of all of WinFLOW software features. The default setting for

a Windows XP operating system is NO users. Therefore, change permissions toEveryone after installing WinFLOW on the computer.

1. Enable Full Control to Everyone by right-clicking Startat the screens lower left

corner.

2. Click Explore.
http://-/?-http://-/?-


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Installation


Rev. 2.3

3. Find the Folder named Flow_4.

NOTE: WinFLOW normally installs in this folder. If the software installed in a

different location, go to that folder.

4. Right-click the Flow_4 folder and select Properties.

5. Click the Securitytab and add the group Everyone if not already created

(Figure 2.3).

Figure 2.3. Permissions dialog box

6. Click the checkbox under Allownext to Full Controlto allow everyone full control

permissions.

7. Click Applyand OKto exit the screen.
http://-/?-http://-/?-


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Rev. 2.3

Ch a p t e r 3 Configuration

After installing WinFLOW, configure communications to the analyzer and autosampler.

Configuring WinFLOW

1. Access the Configuration Editor by selecting FLOW config 4.2from the Start

menu. Click Programs, WinFLOW, and then the FLOW config icon (or go to

Tools Configuration in WinFLOW). The Configure WinFLOW dialog box appears

(see Figure 3.1).

Figure 3.1. Configure WinFLOW dialog box

2. Under Configure Software, select the appropriate language for WinFLOW to

display for its titles, buttons, labels, etc. The language options are English, French,

Dutch, and Spanish.
http://-/?-http://-/?-


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Configuration


Rev. 2.3

3. Click the Edit Facility Informationbutton to enter a facility name and department

to display on hard-copy reports.

4. Select the Create Debug Filescheckbox to generate text files that assist OI

Analytical support personnel with troubleshooting software and performance

problems.

5. Select the communication port (Com Port), Sampler Control, and the analyzer

type (Platform) to use. Only available communication ports appear as choices.

6. Select the appropriate Sampler Control.

a. Select Standaloneif using an autosampler not listed or if no autosampler is

used. If using an autosampler not listed, the autosamplers timing cycle must

match WinFLOW. The autosampler and data collection must initiate at the

same time.

b. Select RA to use vials and vial racks provided with the RA Sampler. The RA

Sampler supports any combination of three trays: 21, 24, 40, 60, or 90

positions; the RA sampler also supports the Autodilutor.

c. Select 3360to use vials and vial racks provided with the Model 3360 Sampler.

The Model 3360 supports any combination of four 60-position and 90-position

racks; the Model 3360 also supports the Autodilutor.

d. Select 3090to use vials and vial racks provided with the Model 3090 Sampler;

the Model 3090 supports either one 60-position or one 90-position rack.

e. Click the Configure Samplerbutton to set the sampler to utilize up to four

different types of sample cups, configure any dilutor in use, specify the desiredrack options, and define alternate locations for wash and diluent fluids.

7. Check the Auto 502 check box, if appropriate:

If using a Flow Solution IV or IVsystem, check the Auto 502checkbox to control

the Precision Pump through the Instrument Control dialog box or the Auto Events

Scheduler (see Chapter 4, Tools Menu on page 27).

If using an Flow Solution 3100 system, select the Auto 502checkbox to control the

Precision Pump.

The CNSolution FS 3100 uses the Model 3090 Autosampler. Flow Solution 3100

systems use either the Model 3090 or Model 3360 Autosampler. Flow Solution III

and IV systems use any of the samplers, including the RA, Model 3090, and Model

3360. Run upgraded systems without one of the listed autosamplers in the standal-

one sampler control mode, provided the autosampler being used is capable of inde-

pendent operation.

NOTE: Configuring WinFLOW every time the software opens is not necessary.

Change WinFLOW configuration settings only if altering the instrument

configuration.


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Configuration


Rev. 2.3

8. After selecting the checkbox to control the Precision Pump, use the Pump Turns

pulldown menu to select the pump direction (when viewed from the front of the

pump). Leftindicates the negative pump direction (the pump pushes the liquids

clockwise), while Rightindicates the positive direction (counter-clockwise).

Most Flow Solution IV customers will select Right. Most Flow Solution 3100 cus-

tomers will select Left.

Configuring the RA Sampler

1. Select the RA sampler in the Configure WinFLOW dialog box.

2. Click Configure Sampler. The RA Configuration dialog box appears as shown in

Figure 3.2.

Figure 3.2. RA Configuration dialog box
http://-/?-http://-/?-


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Configuration


Rev. 2.3

Vial Depths Using a Traveling Wash Reservoi r (TWR)

Adjust the depth that the sample probe travels into a sample cup.

1. Click Vial Depthsin the RA Configuration dialog box. The Vial Depths dialog box

appears (see Figure 3.3).

Figure 3.3. Vial Depths dialog box

2. Enter the appropriate vial depths (in millimeters) in the corresponding boxes. The

maximum probe depth in the sample vial is approximately 95 mm.

Using a Static Wash Reservoir (No TWR)

Adjust the depth that the sample probe travels into a sample cup.

Due to variations of individual sampler setups, no exact default depths are available,

although starting depths are provided in Table 3.1 on page 11. Instead, the process iscompleted by trial and error where the optimal probe positions are determined for each

vial.

Any of the Vial Type numbers can be assigned to any of the vials available (2 mL,

4 mL, 8 mL, and 12 mL). Follow the steps below to configure the vial depths for each

Vial Type.


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Configuration


Rev. 2.3

1. Click Vial Depthsin the RA Configuration dialog box. The Vial Depths dialog box

appears (see Figure 3.3).


2. Enter the appropriate vial depths (in millimeters) in the corresponding boxes. Ifusing a static wash reservoir (no TWR), the range depends on the setup probe

position in the autosampler arm and the wash reservoir type being used; the range is

generally larger than 95 mm.

To get the maximum probe depth into the vial, set the probe at its uppermost posi-

tion and adjust the needle so that the tip of the needle barely clears the lip of the

wash reservoir. Lock the needle into position with a set screw.

Figure 3.5. Probe needle in relation to the wash reservoir

a. Determine which vial size will be used the most (from 2 mL, 4 mL, 8 mL, and

12 mL). This value normally designates Vial Type 1.

b. Verify Vial Type 1is set as the Default Vial Type in the Method Editor (see

Chapter 5, Flow Control Settings Screen on page 38).

Table 3.1. Vial Depth starting values

Vial Size

(mL)Rack Size

Approx imate

Starting Value (mm)

2 90-position 65

4 60-position with shelf 75

8 90-position 100

12 60-position 100

Needle

WashReservoir

Just clear the

reservoir


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Configuration


Rev. 2.3

c. Open the WinFLOW software and create a Sample Table with a cup position

adjacent to the position that contains a vial of Vial Type 1.

d. Send the sampler to the position chosen in step 1 by starting a run. Check the

depth of the probe tip to determine whether to raise, lower, or leave the tip.

NOTE: For 8-mL and 12-mL tubes:If the probe goes over the vial, but doesn't godown, the depth is set deeper than the sampler can go. Decrease the depth

until the probe will lower. This value is the maximum depth for any vial; the

maximum possible depth is 100mm.

e. If the depth needs adjustment, close WinFLOW and open Configure Winflow.

Make the appropriate depth changes and click OK.

f. Open Winflow and repeat steps dthrough euntil the optimal depth is reached.

g. Repeat steps athrough ffor each Vial Type.

NOTE: If only using one vial type, only set the depth for the default Vial Type.

NOTE: Vial types other than the default must be designated with their type number in

the Vialcolumn of the Sample Table for each non-default vial position.

Dilutor Setup If using the Autodilutor with the RA Sampler, click Dilutor Setup in the RAConfiguration dialog box. The Dilutor Setup dialog box appears (see Figure 3.6).

Figure 3.6. Dilutor Setup dialog box

By selecting Use Dilutor, Syringe Size and Syringe Speed settings become available

for input.

Use the pull-down menu to select the syringe size. The default syringe size is 2,500 L.

The available syringe sizes include the following: 1,000 L, 2,500 L, 5,000 L, and

10,000 L.


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Configuration


Rev. 2.3

Syringe size and the application determine proper syringe speed. The speed ranges from

25 for larger syringes to 50 for smaller syringes. The default speed values for each

syringe size are shown in Table 3.2.

Wash and Diluent OI Analytical offers two RA Sampler probe arm configurations. Specify which type touse by clicking Wash and Diluentin the RA Configuration dialog box. The Wash and

Diluent dialog box appears (Figure 3.7).

Figure 3.7. Wash and Diluent dialog box

1. Choose a Wash Source:

TWR(traveling wash reservoir)

Reservoir(fixed reservoir)

Cup Number(any specified cup location)

Most applications recommend using the TWR as the wash source. This configura-

tion allows a two-stage rinsing process.

Use the fixed reservoir as the recommended alternative to the TWR. Using a speci-

fied cup or vial location as the wash source is possible, but not recommended due to

volume limitations.

Table 3.2. Recommended Values for Syringe Sizes

Syringe Size(L)

RecommendedSpeed

Recommended CupSize

Rack Size forRecommended Cup

1,000 50 2-mL 90-position

2,500 35 4-mL 60-position with shelf

5,000 25 8-mL 90-position

10,000 22 12-mL 60-position


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Configuration


Rev. 2.3

2. Choose a Diluent Source:

Reservoir

Cup Number

3. Click OKto close the Wash and Diluent dialog box.

Rack Placement Setup the racks being used with the RA Sampler by clicking Rack Placement in theRA Configuration dialog box.The Rack Placement dialog box appears (Figure 3.8).

Figure 3.8. Rack Placement dialog box

The RA Samplers default configuration uses three sample racks and one standards

rack. Use up to six sample racks:

The preprogrammed sample rack sizes are 21, 24, 40, 60, 90, Incubtr2, and

Incubtr3.

The default sample rack size is 90.

The available standard rack sizes are 10 and 20.

The default standards rack size is 20.

Click OKto close the Rack Placement dialog box.


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Configuration


Rev. 2.3

Rack Editor The Rack Editor configures the RA Sampler to use virtually any size or type of rack.Program the rack size and type by clicking Custom Rack Editorfrom the RA

Configuration dialog box. The Rack Editor dialog box appears (Figure 3.9).

Figure 3.9. Rack Editor dialog box


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Configuration


Rev. 2.3

To use racks other than those provided with the RA Sampler, measure the rack and vial

slot dimensions and the distance between each vial slot. Enter these measurements into

the corresponding locations in the Rack Editor. When saved, custom racks display as an

option in the Rack Placement screen. Create both sample and standard racks using the

following steps.

1. Click Add New Type. Type the custom rack name and click OK.

2. Select Add Cups. The Add Cups dialog box appears (see Figure 3.10).

Figure 3.10. Add Cups dialog box

3. Enter the following information in the corresponding fields:

4. Click OK. Click Close to exit the Rack Editor dialog box.

First Cup #: Number between 1 and 99

Cup Wid (mm): Cup width in millimeters

Number of Cups: Number of rows (left to right) and number of

columns (back to front)

First Cup Position (mm): Distance in millimeters from the standard

trays upper left corner (closest to the samplerbody) to the middle of the first cup

Inter-Cup Spacing (mm): Distance from the cup-to-cup center in

millimeters


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Configuration


Rev. 2.3

5. Click Rack Placementin the RA Sampler Configuration dialog box to access the

Rack Placement dialog box (Figure 3.7).


4. Select the custom rack name just created for the appropriate rack placement. After

selecting a custom rack, the Align Rack dialog box appears as shown in Figure

3.12(if the dialog box does not appear, click the Alignbutton beside the custom

rack).

Figure 3.12. Align Rack dialog box


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Configuration


Rev. 2.3

6. Click OKto accept the default settings or enter new values to adjust the offset. The

RA Sampler rack is now ready and displays the custom rack as shown in Figure

3.13(if the custom rack screen does not appear, click OK to close the Rack

Placement dialog box and the Custom Rack Editor button).

Figure 3.13. Rack Editor dialog box displays a custom RA Sampler rack ready for use

Configuring the 3360 Sampler

1. Select 3360in the Configure WinFLOW dialog box.

2. Click Configure Sampler. The 3090/3360 Configuration dialog box appears(Figure 3.14).

Figure 3.14. 3090/3360 Configuration dialog box
http://-/?-http://-/?-http://-/?-http://-/?-http://-/?-http://-/?-


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Configuration


Rev. 2.3

Vial Depths Adjust the depth that the sample probe travels into a sample cup.

1. Click Vial Depths. The Vial Depths dialog box appears (Figure 3.15).


Enter the desired vial depths (in millimeters) in the corresponding boxes. The depths

correspond to the depth of the needle when used with the following cups:

Other settings may be used if other types of cups are used in the sampler. This

information is utilized in the sample table, to allow the operator to include multiple cup

types in a single run.

Table 3.3. Vial Depths

Cup TypeDefault Depth

(mm)

Cup Dimensions

(mm)

Cup Capacity

(mL)

1 150 13 x 100 8

2 93 12 x 25 2

3 113 12 x 30 4

4 150 16 x 100 12
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Configuration


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Dilutor Setup If using the Autodilutor with the 3360 Sampler, click Dilutor Setupin the 3090/3360Configuration dialog box. The Dilutor Setup dialog box appears (Figure 3.16).

Figure 3.16. Dilutor Setup dialog box

By selecting Use Dilutor, Syringe Size and Syringe Speed settings become available

for input.

Use the pull-down menu to select the syringe size. The default syringe size is 2,500 L.

Syringe size and the application determine proper syringe speed. Table 3.4shows the

recommended speeds for each syringe size, as well as the cup size that is typically

utilized for each syringe size.

Table 3.4. Recommended Values for Syringe Sizes

Syringe Size

(L)

Recommended

Speed

Recommended Cup

Size and Type

Rack Size for

Recommended Cup

2,500 23 4-mL type 38-mL type 1

12 mL type 4

60-position with shelf or90-position
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Configuration


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Wash and Diluent To provide an alternate source of wash or diluent solution, click the Wash and Diluentbutton. The Wash and Diluent dialog box appears (Figure 3.17).


1. Choose Reservoirto draw wash/diluent from the rinse station.

2. Click Cup Numberand supply a cup number and cup type if an alternate solution

is needed.

Using a specified cup or vial location as the wash source is possible, but not recom-

mended due to volume limitations.

Rack Placement Setup the racks being used with the 3360 Sampler by clicking Rack Placementin the3090/3360 Configuration dialog box. The Rack Placement dialog box appears (Figure

3.18).

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Configuration


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The 3360 Sampler uses four sample racks and one standards rack.

The preprogrammed sample rack sizes are 60 and 90.


The available standard rack sizes are 10 and 12.

The default standards rack size is 10.

Peck Probe duringRinse

Check this checkbox to rinse the sampler probe by pecking. Pecking is where the probe

is plunged into and out of the rinse position three times between samples. This rinse

method is typically used with SFA.

Leave this box unchecked in order to rinse the probe by simply lowering it into the rinse

position. This rinse method is typically used with FIA.

Configuring the 3090 Sampler

1. Select 3090 in the Configure WinFLOW dialog box.

2. Click Configure Sampler. The 3090/3360 Configuration dialog box appears

(Figure 3.19).

Figure 3.19. 3090/3360 Configuration dialog box


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Configuration


Rev. 2.3

Vial Depths Adjust the depth that the sample probe travels into a sample cup.

1. Click Vial Depthsin the 3090/3360 Configuration dialog box. The Vial Depths

dialog box appears (Figure 3.20).


2. Enter the appropriate vial depths (in millimeters) in the corresponding boxes. The

vial depths correspond to the depth of the needle when used with the following

cups:

Other settings may be used if other types of cups are used in the sampler. This

information is utilized in the sample table to allow the operator to include multiple cup

types in a single run.

3. Click OKto close the Vial Depths dialog box.

Table 3.5. Vial Depths

Cup TypeDefault Depth

(mm)

Cup Dimensions

(mm)

Cup Capacity

(mL)

1 150 13 x 100 8

2 93 12 x 25 2

3 113 12 x 30 4

4 150 16 x 100 12


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Configuration


Rev. 2.3

Wash and Diluent To provide an alternate source of wash or diluent solution, click the Wash and Diluentbutton. The Wash and Diluent dialog box appears (Figure 3.21).


1. Select a Wash Source:

a. Choose Reservoirto draw wash from the rinse station.

b. Click Cup Numberand supply a cup number and cup type if an alternate

solution is needed.

Using a specified cup or vial location as the wash source is possible, but not

recommended due to volume limitations.

2. Choose a Diluent Source.

a. Choose Reservoirto draw diluent from the rinse station.

b. Click Cup Numberand supply a cup number and cup type if an alternate

solution is needed.


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Configuration


Rev. 2.3

Rack Placement Setup the racks being used with the 3090 Sampler by clicking Rack Placementin the3090/3360 Configuration dialog box. The Rack Placement dialog box appears (Figure

3.22).


The 3090 uses a single sample rack and one standards rack.

The supported sample rack sizes are 60 and 90.


The only supported standard rack sizes is a 10-position rack.

Peck Probe during

Rinse

Check this checkbox to rinse the sampler probe by pecking. Pecking is where the probe

is plunged into and out of the rinse position three times between samples. This rinsemethod is typically used with SFA.

Leave this box unchecked in order to rinse the probe by simply lowering it into the rinse

position. This rinse method is typically used with FIA.


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Rev. 2.3

Ch a p t e r 4 Introduction to

WinFLOW

Starting WinFLOW

1. Double-click the WinFLOW icon or select WinFLOWfrom the Start menu.

2. Enter the serial number included in the WinFLOW documentation.

NOTE: WinFLOW only requests the serial number the first time the software starts.

3. If password protection is enabled for program startup, enter the user name and

password, and click OK. For more information on password protection see

System Security in this chapter.

NOTE: All instrument modules must be properly powered on and initialized for

online operation.

Select View DialogBox

WinFLOW displays the Select View dialog box on startup (Figure 4.1).

Figure 4.1. Select View dialog box

The Select View dialog box allows easy, single-click access to WinFLOWs four

primary options. It also provides an icon to exit WinFLOW. Table 4.1outlines

WinFLOWs four main options.
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Introduction to WinFLOW


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When the Select View dialog box appears, the main menu bar displays File, Tools, and

Window menus, which can also be accessed from any WinFLOW screen. The File

menu also provides an Exit option.

Tools Menu

The Toolsmenu provides access to screens that establish WinFLOW preferences,

configure instruments, and set pump speeds, valve states, and heater temperatures.

Figure 4.2. Tools Menu Preferencesaccesses the Preferences dialog box.

Configurationaccesses the Configuration dialog box.

Auto Event Scheduleraccesses the Event Scheduler dialog box.

Instrument Controlaccesses the Instrument Control dialog box.

Pump Timerdisplays the number of hours the pumps are used.

Table 4.1. WinFLOW main options

Main Option Select ViewIcon

ShortcutKey

Small Icon Function

Collect Data F2 Initiates data collection following

sample table and method selection.

Sample Table F3 Opens a table displaying sample

cup number, name, type, replicate,

dilution, and weight factors.

Method Editor F4 Sets data collection parameters and

calibrant and QC information.

Data Analysis F5 Accesses results from any

previously saved run. Contains

peak plot, peak table, calibration

plot, and calibration data tables.

Exit F6 N/A Exits WinFLOW.


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Rev. 2.3

Preferences Set global WinFLOW preferences such as default views and languages using thePreferences dialog box (Figure 4.3).

Figure 4.3. Preferences dialog box

Configuration The Configuration dialog box (Figure 4.4) provides information about the instrument

platform, such as the autosampler installed with the system, the firmware version, andthe number of communicating detectors (channels), valves, and pumps.

The Configuration dialog box allows the operator to determine if the analyzer, as well

as each installed component, communicates with the computer. Selecting Configure

when the analyzer or a component loses communication to re-establish a link between it

and the computer.

Security Sets WinFLOW to request a password on startup. For more

information see System Security on page 33in this

chapter.

Save Views Sets WinFLOW to display any screen on startup that

remained open when WinFLOW closed.

NOTE: Save Views does not bring up results and analysis files left open when

exiting.

Verify Close Sets WinFLOW to automatically prompt to verify theoperators intention to exit the program.

Re-enable Helpers Implements various warning screens that can be disabled

as operators gain experience with WinFLOW.

Language Select from English, French, German, or Spanish. Contact

your local OI Analytical distributor for more information

about other available languages.


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Rev. 2.3

Figure 4.4. Configuration dialog box

Events Scheduler The Events Scheduler dialog box (Figure 4.5) sets what action(s) the pump(s) take(s)when any of the specified events occur, such as the end of an analysis.

Figure 4.5. Events Scheduler dialog box

Add Selects the event, time, and action.

Edit Edits any previously chosen event.

Remove Removes any previously chosen event.


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Rev. 2.3

Instrument Control Manually set pump speeds, valve states, and heater temperatures from the InstrumentControl dialog box (Figure 4.6).

NOTE: The heater temperature settings do not control the Power Base cartridge

heaters.

Figure 4.6. Instrument Control dialog box

Pump Timer The Pump Timer dialog box (Figure 4.6) indicates the number of hours the pump(s)run. Use this feature to determine when consumables, such as pump tubes and

membranes, need replacing. Reset the pump timer at any time.

Figure 4.7. Pump Timer dialog box

Window Menu

The Windowmenu contains options for displaying open windows within WinFLOW.

The Window menu lists all open windows. Access any open window from the Window

menu by selecting the name or entering the number associated with the window.

Figure 4.8. Window menu Cascadeoverlaps open windows.

Tileplaces open windows side by side.

Arrange Iconsplaces minimized WinFLOW screens along the bottom the Select View

dialog box.


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Rev. 2.3

About Menu

The Aboutmenu contains information about software and firmware versions, as well as

provides technical support access numbers.

Shortcut Keys

WinFLOW contains several keyboard shortcuts. These function keys provide an

additional method for locating and enabling various software activities. Table 4.2

describes the available function keys.

These function keys duplicate the Select Viewicons, which are also available from the

icon ribbon or the pulldown menu. Therefore, WinFLOW provides multiple avenues for

accessing the most frequently used software features.

Icon Ribbon

The following list details the various icons and their purposes.

Table 4.2. Shortcut keys

Function Key Description

F2 Opens the Data Collection dialog box.

F3 Opens the Sample Table dialog box.

F4 Opens the Method Editor dialog box.

F5 Opens the Data Analysis dialog box.

F12 Exits WinFLOW.

Opens the Data Collection screen.

Opens the Sample Table screen.

Opens the Method Editor screen.

Opens the Data Analysis screen.

Initiates data display.


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Rev. 2.3

Resets to the baseline views beginning, which deletes all data collected.

Fast forward icon, which initiates data collection.

Pauses data collection.

Stops data collection.

Zeros the baseline signal on the peak plot.

Removes or restores the grid lines in the peak plot and calibration plot.

Peak plot update lock freezes the baseline update used during data

collection.

Activates the peak editor cross.

Deletes or reprocesses peaks.

Recalculates results.


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Rev. 2.3

System Security

System security reduces unauthorized WinFLOW software access or use. It provides

simple, password-protected access to WinFLOW and some of its features. Accessing

the Security screen alwaysrequires a password.

If the system password is lost, contact OI Analytical Customer Support at(800) 336-1911 or (979) 690-1711.

1. Select Preferencesfrom the Toolsmenu to access the Preferences dialog box

(Figure 4.3). Select the Security checkbox. The password dialog box appears

(Figure 4.9).

Figure 4.9. Password dialog box.

2. Enter the user name and password. Click OKto return to the Preferences dialogbox.

NOTE: Use the default user name and password when first activating the Security

option. The default user name is systemand the default password is

manager. Neither entry is case-sensitive.


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Rev. 2.3

3. Click Set Privilegesto edit, delete, or add a new user name and password. The

Security dialog box appears (Figure 4.10).

Figure 4.10. Security dialog box

4. Select Edit, Delete, or Add, and follow the instructions presented to edit, delete, or

add a user name and password.

WinFLOW Quick Start

NOTE: The following instructions assume all modules and hydraulic connections are

installed.

1. Turn on all Flow Solution modules and the computer.

2. Start the WinFLOW software.

3. Verify all tubing connections are made. Initiate pump(s) rotation by pressing the

pumps Runbutton.

4. Click the Method Editor icon. Verify the method is configured for the analysis to

be performed. Save any changes to the appropriate file.

5. Click the Sample Tableicon. Alter an existing table or create a new sample table.

Save any changes to the appropriate file.

6. Click the Data Collecticon. Select a method and sample table.

7. Enter an operator name, operator ID, and filename for the run results.

8. Click the Play icon and monitor the baseline. Once the baseline stabilizes with

startup solution, switch to reagents and again monitor the baseline until it stabilizes

(typically 1530 minutes).


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Rev. 2.3

9. While the baseline stabilizes, load the autosampler trays according to the sample

table.

10. Click the Fast Forward icon when the baseline stabilizes.

11. Monitor the run until the SYNC peak elutes and is successfully marked. Allow the

run to continue.

If the operator completes the run or the run completes automatically, the data saves

automatically to the result file defined in step 7.

NOTE: Select the Stopicon to stop the run. Save results once the completed run

displays.

12. Review the results.


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Rev. 2.3

Ch a p t e r 5 Method Editor

The WinFLOW Method Editor controls how WinFLOW collects and interprets data.

General Description

Open the Method Editor using one of the following ways:

Click the Method Editor icon from the Select View dialog box,

Click the Method Editor icon on the main icon ribbon, or

Press [F4].

Depending on the installed hardware, the Method Editor includes screens that modify

all method parameters necessary for running an analysis. Use the following six screenswith flow analysis hardware:

Flow Control Settings

Detector/Channel Settings

Signal Filter and Marking Settings

Calibration/Quantitation Settings

Timed Events Editor

Calibrants Table

This chapter discusses each of these screens and their associated menus. After setting

parameters properly, these screens do not require modifying during future analyses for

specific analytes.

Locate each screens title at the top of the window. The window bar also contains the

open methods name. Access each screen using one of the following ways:

Click the screens icon in the Method Editor icon ribbon,

Click the Method Editor icon ribbons advance or reverse arrow until the desired

screen appears, or

Go to the Method Editors Page menu, which lists each of the screens separatelyalong with their associated quick key functions.


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Method Editor


Rev. 2.3

Method Editor IconRibbon

Opening a Method A method automatically opens when accessing the Method Editor. Open a previouslysaved method or an installed method using the following steps:

1. Go to theFilemenu and select Open.

2. Select the method to open, then click OK.

NOTE: If the methods platform is not the same as the current platform, a dialog box

appears warning that other method options need updating to match the

platform selected. If selecting Yes, WinFLOW adapts some method

parameters to allow WinFLOW to work on the current platform.

CAUTION:

Always use Save Aswhenaltering an existing

method so the original

does not become

overwritten.

3. After the method loads, modify it if necessary. See Creating a Method on page 38in this chapter.

Advances to the next screen.

Accesses the Flow Control Settings screen.

Accesses the Detector/Channel Settings screen.

Accesses the Signal Filter/Marking Settings screen.

Accesses the Calibration/Quantitation Settings screen.

Accesses the Timed Events Editor screen.

Accesses the Calibrants Table screen.

Returns to the previous screen.


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Method Editor


Rev. 2.3

Creating a Method The fastest way to create a method consists of altering an existing method and thenusing Save Asto save the new method under a new filename. WinFLOW installs with

the following six preset methods: single and dual methods for the Flow Solution 3000

(FS 3000) and the Flow Solution IV (FS IV), and a three-channel method for the FS IV.

Use these methods as templates for creating new methods. Most parameters in the

preset methods do not need changing when creating a new method for a given

chemistry.

Saving a Method When altering a method and the changes have not been previously saved, the Save Asdialog box automatically appears when another method opens.

NOTE: Saving a modified method as its original name overwrites the original settings

in that method. Save As modifies a method under a different name to maintain

the original settings.

Flow Control Settings ScreenSelect an instrument, activate channels, enable the baseline verification function, set the

sampling mode, set the default vial type (when using the Model 3090, Model 3360, or

RA Sampler), and define pump speeds from the Flow Control Settings screen

(Figure 5.1). Access the Flow Control Settings screen by clicking its icon from the

Method Editor icon ribbon, by going to the Pagemenu and selecting Flow Control, or

by pressing [Shift]+[F1].

Figure 5.1. Flow Control Settings screen


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Method Editor


Rev. 2.3

Instrument Specify one of three instrument platform groups:

FS III/IV/3100

FS 3000

Microbes-Coli

If the platform changes, a warning message displays stating that parameters need

updating to reflect the new platform.

NOTE: Changing the instrument selected does not automatically change the hardware

configuration. Although methods for any platform can be created at any time,

a method cannot be used unless WinFLOW is configured to function with the

current hardware (see Chapter 3, Configuration).

Sampling Mode Set Sampling Mode to one of two options:

Use Single Sample mode by performing the following steps:

1. Select Single Sample as the Sampling Mode in the Flow Control Settings screen.

2. Proceed with data collection as described in Chapter 7, Data Collection on

page 69.

At the point of data collection, the Single Sample Mode dialog box appears

(Figure 5.2).

Figure 5.2. Single Sample Mode dialog box

Uninterrupted Allows operation with an integrated autosampler. All samples in

a sample table analyze in a single, uninterrupted run.

Single Sample Allows flow injection analysis (FIA) operation with or without

an autosampler, or continuous flow analysis operation with an

autosampler. Each sample loads and injects when the operator

chooses.

Interval Currently unavailable.


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Method Editor


Rev. 2.3

3. For FIA operation without an autosampler, manually place the sample needle into

the sample or standard solution and click Sample. The sample or standard aspirates

into the sample loop for the time specified in the timed events table (Figure 5.8)

(see Timed Events Editor Screen on page 52in this chapter). When the loop

valve rotates to the inject position, place the sample needle into the wash source.

For FIA or segmented flow analysis (SFA)-continuous flow operation with anautosampler, click Samplewhen the next sample standard is ready. The

autosampler automatically places the sample needle into the sample or standard vial

and extracts the sample or standard for the time specified in the timed events table

(Figure 5.8). The sample needle then returns to the wash source.

Figure 5.2displays after each samples cycle time completes until all the samples in

the sample table are processed.

The operator can also use a blank table each time a sample or standard aspirates.

1. Select Generate Blank Tablein the Run Setup dialog box, which appears when

data collection initiates (Figure 5.3).

Figure 5.3. Selecting the single sample blank table option

2. When the Single Sample Mode dialog box appears (Figure 5.2), click Edit. A blank

table appears.

3. Fill in the next sample or standard and close the window. WinFLOW prompts to

save the table. Saving the table automatically begins the analysis.


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Method Editor


Rev. 2.3

CCV/Drift Fail Automatic recalibration options require deciding on a system action following a failedcontinuing calibration verification (CCV). For more information on CCVs, see

Continuing Calibration Verification on page 56in this chapter.

Vial Types Set Vial Types when using the Model 3090, Model 3360, or RA Samplers. WinFLOWcan use four operator-defined vial types within each analysis. Define each vial types

depth in the WinFLOW Configuration dialog box (see Chapter 3, Configuration on

page 7). Set the default vial type to represent the majority of the operator-defined vial

types used.

Off-scale (HI)Samples

Set autodilution options when using the Model 3360 or RA Samplers with the

Autodilutor. Enable autodilution by selecting Enable Autodilution. Once autodilution

enables, the following options become available:

Flag Only Places an F in the results tables flag column.

Pause Run Flags the calibrant and aborts the analysis.

Recalibrate Flags the calibrant and reruns the calibration section. Sampling

resumes at the cup after the last successful CCV.

Dilution Factors The Model 511 Autodilutor contains hardware-fixed

dilution factors.

The Autodilutor allows selecting a continuously variable

set of dilution factors. Enter up to five dilution factors,

each separated by commas. The Autodilutor uses the first

entered dilution factor for any sample that goes over thehighest calibration standard concentration. The autodilutor

uses subsequent operator-defined dilution factors if the

diluted sample concentration is still too high. Although the

autodilutor has a 1:24,000-step resolution, dilution factors

greater than 200 should not be used.

CCV Frequency Chooses the frequency of running CCVs with the diluted

samples (e.g., run a CCV every 10 samples). Designate the

CCV in the sample table after the dilution cups (see

Figure 6.1 on page 58and Table 6.2 on page 60).

Number of

Followers

Selects the number of followers to be run. A follower is the

sample(s) after an over-limit (HI) sample. Therecommended setting is two followers.


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Method Editor


Rev. 2.3

Insert Baselines Insert wash baseline (WBL) points at regular intervals. A WBL point forces thebaseline through that point. When using the RA, Model 3090, and Model 3360

samplers in a segmented system, the WBL sample is taken from the wash reservoir

instead of a sample cup. For a FIA system, the sample probe stays in the wash, but the

valve does not inject, and carrier solution determines the baseline. Enable baseline

verification by selecting Enable under Insert Baselines, then set the desired First

position, Interval, and Duration.

When enabled, the baseline verification operates on all the activated channels. Baseline

verification points, which are designated WBL, do not appear in the sample table, but

they do appear in the sequence table preview.

Pump Control Control pump speed when operating the Precision Pump with the FS 3100, FS 3000,and CNSolution analyzers. Set these values in 1% increments.

Automated Precision Pump Control Opt ions

Refer to the method for the operating speed of the pump for the individual application.

Operate the Precision Pump at 40% speed in a counterclockwise direction

(40 setting) for most applications. Typically use a 10% slow speed (10 setting).

Automated FS 3000 and CNSolution 3000 Pump Control Opt ions

The FS 3000 and CNSolution 3000 pump typically operates at 100% full speed in aclockwise direction with the pump speed switch in the low position. A common slow

speed setting is 50%, which means the pump turns at full speed 50% of the time and

turns off 50% of the time. For a 30-second cycle time and a 50% slow speed setting, the

pump operates for 15 seconds and then stops for 15 seconds.

Start Position Provides the sample table location where the first baseline

verification point occurs. Check the sequence table preview in the

Data Collection screen to verify correct placement.

Interval Sets the number of samples between baseline verifications (250).

Duration Sets the number of baseline verifications to run consecutively (15).

Run Speed Sets the desired pump speed during normal operation.

Slow Speed Sets the desired pump speed used when the Pump Slow command

activates either manually or automatically. For manual operation,

see Chapter 4, Instrument Control on page 30. For automatic

operation, see Chapter 4, Events Scheduler on page 29.


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Method Editor


Rev. 2.3

Detector/Channel Settings Screen

The Detector/Channel Settings screen (Figure 5.4) sets parameters for each detector

channel individually. Update information for a particular channel by selecting the

channels line in the Channels list box. The information on the screen pertains only to

the highlighted channel. Only available channels appear in the Detector/Channel

Settings screen. Access the Detector/Channel Settings screen by clicking its icon fromthe Method Editor icon ribbon, by going to the Pagemenu and selectingDetector/

Channel, or by pressing [Shift]+[F2].

Figure 5.4. Detector/Channel Settings screen

Channels The Channelslistbox displays all the available channels. The number of availablechannels depends on the number of installed detectors. A channel presents as available

following successful communication with a detector.

Activate a channel by double-clicking the channel name. A number sign (#) sign

appears to the left of the activated channel name (Figure 5.4). Deactivate a channel by

double-clicking the channel name again. Method parameters only apply to active

channels. Therefore, channels must be activated before moving to other Method Editor

screens.

NOTE: If opening a method that designates an unavailable channel, an X appears

on the channel names left. Re-establish contact with the channel by going tothe Main menu and selecting Setup and Configure. If unsuccessful, the

systems exits and restarts WinFLOW.

Name Designate a nameto describe the chemistry being run. WinFLOW uses the channeldesignation (e.g., Channel 1) as the default name.


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Method Editor


Rev. 2.3

Methodology Designate a methodology that describe the specific chemistry being run.

CAS Number Enter the Chemical Abstract Service (CAS) number for the standard used in theselected channel, if applicable.

Detector Settings

Mode Sets the detector mode as Photometric, Amperometric, ISE

(ion-selective electrode), Fluorometric, or Matrix(seawater

matrix). Select Photometric for the Expanded Range(ER)

detector. Select Amperometric for the Amperometric detector

and for the analog-to-digital (A/D) converter. Use ISE for all ion-

selective probes such as pH or fluoride. Use Fluorometric with

the fluorometric plug-in detector.

Acquisition Rate Determines the data collection rate in Hz (or points per second).

Options include 1, 2, 3, 5, or 10 Hz. The 1-Hz default valueusually suffices. When running very low analyte levels,

increasing the acquisition rate can result in a sensitivity increase.

When operating multiple channels, set the acquisition rate to 1

Hz so the computer does not overload with data.

Cell Potential Set the cell potential in amperometric mode using the detectors

10-turn precision potentiometer. Setting cell potential via

software is a future option.

A-D Gain Sets the analog-to-digital gain for the sample and reference.

Options include 1, 2, 4, 8, 16, 32, 64, 128, or Auto. Set Sample to

Autofor all photometric detectors. The photometric detectorautomatically sets the optimum gain based on the amount of light

reaching the photodiode. Set the amperometric detector to a fixed

gain of 1.

Set Reference to Auto for all photometric detectors. The

photometric detector automatically sets the optimum gain based

on the amount of light reaching the photodiode. The

amperometric detector does not have a reference gain.


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Method Editor


Rev. 2.3

ChannelSubtractions

Subtract peak concentration, peak height, or area in one channel from peak

concentration, peak height, or area in another channel. For example, nitrate-plus-nitrite

analysis typically occurs on one channel and nitrite-only analysis on another. Use the

Channel Subtractions function for automatically calculating nitrate concentration by

subtracting nitrite concentration from nitrate-plus-nitrite concentration. Enable Channel

Subtractions on only one of the two channels because peak concentrations in one

channel subtract from peak concentrations in another channel. For example, channel 1peak concentrations (nitrite) subtract from channel 2 concentrations (nitrate-plus-

nitrite). Therefore, enable Channel Subtractions on channel 1 and not channel 2.

Subtracted data (nitrate) appears with other data in the peak results table.

NOTE: When using Channel Subtractions, OI Analytical recommends using

concentrations rather than peak height or area.

Subtracted From

Channel

Selects the channel from which the current channel subtracts.

In the nitrate-plus-nitrite example, the Subtract From Channel

is the nitrate-plus-nitrite channel (channel 2).

Subtraction Name Designates the subtracted datas name. For example, if nitrite

peak concentrations subtract from nitrate-plus-nitrite peak

concentrations, name the subtracted data Nitrate.


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Method Editor


Rev. 2.3

Signal Filter and Marking Settings Screen

Set smoothing, baseline marking, and peak marking options for each channel

individually from the Signal Filter and Marking Settings screen (Figure 5.5).

Access the Signal Filter and Marking Settings screen by clicking its icon from the

Method Editor icon ribbon, by going to the Pagemenu and selecting Signal Filter/Peak Marking, or by pressing [Shift]+[F3].

Figure 5.5. Signal Filter and Marking Settings screen


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Method Editor


Rev. 2.3

Smoothing Select one of three software-based signal processing algorithms to reduce noise,improve precision, and ease integrating difficult peaks (small size relative to noise).

Choose None, Moving Average, Olympic, or Savitzky-Golay. Select one of these

algorithms in advance. If selecting None, access these algorithms after the run

completes and apply them in the results file.

Moving Average Selects a simple algorithm for filtering where the average of an

input set is used to generate one output value. This algorithm

tends to reduce peak height maxima due to the lower values on

either side of the peak. Peak area measurements remain largely

unaffected. Use a maximum of nine data points and one

iteration for best results with this algorithm.

Olympic Selects a variation of the moving average algorithm. It

eliminates high and low values and averages the remaining

values. This algorithm very effectively screens out spikes (high

frequency noise). Like moving average, the olympic algorithm

tends to reduce peak height. Peak area measurements remain

largely unaffected. Use seven data points (11 maximum) and

one iteration for best results with this algorithm when acquiring

data at 1 Hz.

Savitzky-Golay Selects an algorithm that multiplies each point by a weighting

factor and then averages the points in a data set. The factors,

derived from second- and third-degree polynomials, weight the

center of the data point set and progressively lessen the

weighting to the points further out. Unlike the other algorithms,

this setting minimizes peak height maxima distortions. Peak

area measurements also remain unaffected. Use several data

points and multiple iterations for best results with this

algorithm when acquiring data at 1 Hz.

Points Defines the number of input data points processed to generate

each output data point. Options include 3, 5, 7, 9, 11, 13, and

15. The data acquisition rate is a very important parameter in

determining the optimum number of points for a given peak

width. The default setting uses five data points, which is

adequate for most applications. Use a higher value when

processing extremely wide peaks.

Iterations Sets the repetition number that the algorithm treats the output

of one operation (filtering algorithm) as the input for the next

operation. It increases the degree of filtering (smoothing) whilekeeping the filter width (points) optimized for the peak width.


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Method Editor


Rev. 2.3

Baseline Marking Baseline Markingconsists of the following three parameters:

InvertedChemistry

Select Inverted Chemistry when the chemistry being run measures a decrease in

response instead of an increase. Select this option only if the analytical method for the

chemistry used states that it is an inverse chemistry.

NOTE: This feature can only be used with certain detector types.

Peak Marking

Lead Time Sets the time in seconds before the SYNC peak, which is used

as the initial baseline point. WinFLOW determines the

baseline lead after marking the SYNC peak by counting

backward from the SYNC peak marker. Be sure to set the

baseline lead time longer than half the SYNC peaks width so

WinFLOW does not mark the baseline on the rising slope of

the SYNC peak itself. As a general rule, set the baseline lead

time to 15 seconds more than the peak width. The def

winflow4 , análisis de cianuro - analisis intrumental

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