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CONGRESS ORGANIZING BOARDHan WösteHan Wösten, The Netherlands І ChairpersonStanley Brul, The Netherlands Stanley Brul, The Netherlands І Co-ChairpersonTom Coenye, BelgiumTom Coenye, BelgiumGianfranco Donelli, ItalyGianfranco Donelli, ItalyHauke Harms, GermanyHauke Harms, GermanyRoland J. Koerner, United KingdomRoland J. Koerner, United KingdomOscar Kuipers, The NetherlandsOscar Kuipers, The NetherlandsPer-Eric Lindgren, SwedenPer-Eric Lindgren, SwedenJean-Claude Piffaretti, SwitzerlandJean-Claude Piffaretti, Switzerland

CONGRESS PROGRAM COMMITTEEJean-Claude Piffaretti, Switzerland Jean-Claude Piffaretti, Switzerland І ChairpersonStanley Brul, The NetherlandsStanley Brul, The NetherlandsTom Coenye, BelgiumTom Coenye, BelgiumGuy Cornelis, BelgiumGuy Cornelis, BelgiumAlain Filloux, United KingdomAlain Filloux, United KingdomHauke Harms, GermanyHauke Harms, GermanyUrs Jenal, SwitzerlandUrs Jenal, SwitzerlandOscar Kuipers, The NetherlandsOscar Kuipers, The NetherlandsBernhard Schink, GermanyBernhard Schink, GermanyFrank van Kuppeveld, The NetherlandsFrank van Kuppeveld, The NetherlandsHan Wösten, The NetherlandsHan Wösten, The Netherlands

FEMS GRANTS COMMITTEEVaso Taleski, Macedonia Vaso Taleski, Macedonia І ChairpersonJosef Anne, BelgiumJosef Anne, BelgiumHelge Holo, NorwayHelge Holo, NorwayBauke Odega, The Netherlands

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Katrina Forest, USATimothy Foster, IrelandClay Fuqua, USAPierre Genevaux, FranceOlga Genilloud, SpainOlga Genilloud, SpainRicardo Gent, GermanyRicardo Gent, GermanyKenn Gerdes, United KingdomKenn Gerdes, United KingdomJean-Marc Ghigo, FranceJean-Marc Ghigo, FranceNeil Gow, United KingdomNeil Gow, United KingdomAnthony Green, United KingdomAnthony Green, United KingdomAngelika Gründling, United KingdomAngelika Gründling, United KingdomSarah Gurr, United Kingdom Sarah Gurr, United Kingdom Max Haggblom, USAMax Haggblom, USAHauke Harms, GermanyHauke Harms, GermanyHenrik Hasman, DenmarkHenrik Hasman, DenmarkBernhard Hauer, GermanyBernhard Hauer, GermanySophie Helaine, United KingdomSophie Helaine, United KingdomMartin Held, SwitzerlandMartin Held, SwitzerlandKlaas Hellingwerf, The NetherlandsKlaas Hellingwerf, The NetherlandsRegine Hengge, GermanyRegine Hengge, GermanyDavid Holden, United KingdomDavid Holden, United KingdomMartin Howard, United KingdomMartin Howard, United KingdomMary Ann Jabra-Rizk, USAMary Ann Jabra-Rizk, USADick B. Janssen, The NetherlandsDick B. Janssen, The NetherlandsJanet K. Jansson, USAJanet K. Jansson, USAUrs Jenal, SwitzerlandUrs Jenal, SwitzerlandMike Jetten, The NetherlandsMike Jetten, The NetherlandsPatrik R. Jones, United KingdomPatrik R. Jones, United KingdomPilar Junier, SwitzerlandPilar Junier, SwitzerlandJörg Kämper, GermanyJörg Kämper, GermanyDiana Kirilovsky, FranceDiana Kirilovsky, FranceStaffan Kjelleberg, Australia/SingaporeStaffan Kjelleberg, Australia/SingaporeRobbert Kleerebezem, The NetherlandsJan Kok, The NetherlandsOscar Kuipers, The NetherlandsKatja Laufer, Germany Rob Lavigne, BelgiumBruno Lemaître, Switzerland

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Martin Pilhofer, SwitzerlandPatrick Plésiat, FranceCaroline Plugge, The NetherlandsSteven Porter, United KingdomSam Possemiers, BelgiumJack T. Pronk, The NetherlandsJim Prosser, United KingdomStephen Quake, USAWim Quax, The NetherlandsAmparo Querol, SpainJos Raaijmakers, The NetherlandsJos Raaijmakers, The NetherlandsLeonie Raamsdonk, The NetherlandsLeonie Raamsdonk, The NetherlandsKorneel Rabaey, BelgiumPeter Raspor, SloveniaJames Redfern, United KingdomJames Redfern, United KingdomNatalia Requena, GermanyNatalia Requena, GermanyLuigi Rizzo, ItalyJohn Robinson, SwitzerlandJohn Robinson, SwitzerlandPascale Romby, FranceEliora Ron, IsraelPaul Ross, IrelandRamon Rosselló-Móra, SpainRamon Rosselló-Móra, SpainCraig Roy, USAPhilippe Sansonetti, FranceKarin Sauer, USAUwe Sauer, SwitzerlandUlrich Schaible, GermanyBernhard Schink, GermanyJacques Schrenzel, SwitzerlandStephan Seiler, GermanyAngela Sessitsch, AustriaPeter Setlow, USACynthia Sharma, GermanyDavid Sherrat, United KingdomLiz Sockett, United KingdomLotte Søgaard-Andersen, GermanyMorten Sommer, DenmarkGero Steinberg, United Kingdom

Matthew B. Sullivan, USASander Tans, The NetherlandsSander Tans, The NetherlandsRudolf Thauer, GermanyRudolf Thauer, GermanyBart Thomma, The NetherlandsBart Thomma, The NetherlandsJan Tommassen, The NetherlandsJan Tommassen, The NetherlandsArmen Trchnouian, ArmeniaArmen Trchnouian, ArmeniaNatalia Tschowri, GermanyNatalia Tschowri, GermanyUnai Ugalde, SpainUnai Ugalde, SpainIda van der Klei, The NetherlandsIda van der Klei, The NetherlandsJohn van der Oost, The NetherlandsJohn van der Oost, The NetherlandsFrank van Kuppeveld, The NetherlandsFrank van Kuppeveld, The NetherlandsLaurence van Melderen, BelgiumLaurence van Melderen, BelgiumErik van Nimwegen, SwitzerlandErik van Nimwegen, SwitzerlandRonald van Rij, The NetherlandsRonald van Rij, The NetherlandsDouwe van Sinderen, IrelandDouwe van Sinderen, IrelandJan-Willem Veening, The NetherlandsJan-Willem Veening, The NetherlandsStephanus N. Venter, South AfricaStephanus N. Venter, South AfricaVittorio Venturi, ItalyVittorio Venturi, ItalyGerard Verkleij, The NetherlandsGerard Verkleij, The NetherlandsJoanna Verran, United KingdomJoanna Verran, United KingdomPhilippe Villain-Guillot, FrancePhilippe Villain-Guillot, FranceJulia Vorholt, SwitzerlandJulia Vorholt, SwitzerlandGabriel Waksman, United KingdomGabriel Waksman, United KingdomVictoria Wells, United Kingdom Victoria Wells, United Kingdom Cornelia Welte, The NetherlandsCornelia Welte, The NetherlandsWilliam B. Whitman, USAWilliam B. Whitman, USALyle Whyte, CanadaLyle Whyte, CanadaLukas Wick, GermanyLukas Wick, GermanyRob Willems, The NetherlandsRob Willems, The NetherlandsPaul Williams, United KingdomPaul Williams, United KingdomJean-Luc Wolfender, SwitzerlandJean-Luc Wolfender, SwitzerlandHan Wösten, The NetherlandsHan Wösten, The Netherlands

FEMS-0818 Environmental microbiology - 2 BIODEGRADATION OF P-NITROPHENOL BY DIFFERENT MIXED CULTURES M. Ljesevic1, M. Markovic2, G. Gojgic-Cvijovic2, V. Beskoski1, M.M. Vrvic1 1Department of biochemistry, Faculty of chemistry University of Belgrade, Belgrade, Serbia 2Department of chemistry, IChTM University of Belgrade, Belgrade, Serbia Background One of the most important environmental pollutant among nitroaromatic compounds is p-nitrophenol (4-nitrophenol, PNP). It is used in the manufacture of a wide range of products and is the intermediate of some organophosphorus pesticide degradation (1). PNP has been classified as a priority pollutant by United States Environmental Protection Agency (EPA), and its concentration in natural waters is restricted to less than 10 ng/L (2). Objectives The aim of this study was to investigate the biodegradation of PNP by three different mixed cultures (TUS, AM and KOV) isolated from contaminated soil and sediment. Methods The samples were inoculated into minimal medium with increasing PNP concentrations. The biodegradation process was monitored by UV spectroscopy. The toxicity of PNP solution and PNP degradation intermediers to luminescent bacteria Aliivibrio fischeri was evaluated according the ISO 11348-3. Conclusions The mixed culture TUS showed the greatest biodegradation potential. Mixed microbial population was capable of degrading up to 100 mg/L of PNP as a sole carbon and nitrogen source. Toxicity was significantly reduced after the degradation process.

References: 1. Zhang J et al. Journal of Hazardous Materials 163 (2009) 723. 2. Zohar S et al. International Biodeterioration and Biodegradation 84 (2013) 80.

Biodegradation of p-nitrophenol by different mixed cultures

M M. Lješević1,*, B. Kekez1, M. Marković2, G. Gojgić-Cvijović2, V. Beškoski1, M.M. Vrvić1

1 Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11158 Belgrade, P. O. Box 51, Serbia2 IChTM- Department of Chemistry, University of Belgrade, Njegoševa 12, 11158 Belgrade, P. O. Box 473, Serbia

*For correspondence: marijaljesevic@gmail.com

Introduction

One of the most important environmental pollutant among nitroaromaticcompounds is p-nitrophenol (4-nitrophenol, PNP). It is used in themanufacture of a wide range of products and is the intermediate of someorganophosphorus pesticide degradation (1). PNP has been classified as apriority pollutant by United States Environmental Protection Agency(EPA), and its concentration in natural waters is restricted to less than 10ng/L (2).

Methods

The aim of this study was to investigate the biodegradation and detoxification ofPNP by three different mixed cultures (TUS - contaminated sediment from wastemud pit located next to oil wells in Turije, AM - activated sludge from industrialwastewater treatment plant and KOVP - contaminated sediment fromwastewater canal in Pancevo) isolated from contaminated soil and sediment.Samples were inoculated into minimal medium with increasing PNPconcentrations (30, 50, 100 mg/L). The biodegradation process was monitoredby UV spectroscopy. The toxicity of PNP solution and PNP degradationintermediars to luminescent bacteria Aliivibrio fischeri was evaluated accordingthe ISO 11348-3.

Results

Spectrophotometric data indicated that KOVP completely degraded 30 mg/LPNP. Samples with 50 mg/L PNP were fully degraded to the intermediarycompound whose absorption maximum is at 264 nm. KOVP only partiallydegraded 100 mg/L PNP to the intermediary compound with absorptionmaximum at 303 nm (Fig. 1.).Similarly, TUS completely degraded 30 mg/L PNP, while the samples with 50and 100 mg/L PNP were completely converted to intermediary compoundsthat show maximum absorption at 264 and 303 nm, respectively (Fig. 2.).Mixed culture AM converted all concentrations of PNP into an intermediarycompound whose absorption maximum is at 262 nm. In addition to this,samples with 100 mg/L PNP, had another absorption maximum at 353 nm(Fig. 3.).

Conclusion

The mixed culture TUS showed the greatest biodegradation potential. Mixed microbial population was capable of degrading up to 100 mg/L of PNP as a sole carbonand nitrogen source. Toxicity was not detected after the degradation process.

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Fig 1. PNP degradation: mixed culture KOVP

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Fig. 2. PNP degradation: mixed culture TUS

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Fig.3. PNP degradation: mixed culture AM

References

1. Zhang J et al., Journal of Hazardous Materials 163 (2009) 723.2. Zohar S et al., International Biodeterioration & Biodegradation 84 (2013) 80.

Acknowledgement

This work was supported by FEMS grant for European Early Career Scientists andthe Ministry of Education, Science and Technological Development through PhDscholarship and project III 43004.

Presumably, by increasing the PNP concentraction, growth of bacterial culturescapable of complete PNP degradation is inhibited, or if the PNP degradation is aresult of combined action of multiple bacterial culture, the ones who convertintermediary compounds to simple products are inhibited.Five different bacterial strains were isolated from these mixed cultures:Ochrobactrum sp, Shinella sp, Comamonas sp, Ochrobactrum sp andChryseobacterium sp. Identification was performed by sequence analysis of the16s rRNA gene, and the sequences of analysed strains were deposited in NCBIGenBank under accession numbers KF499315, KF499316, KF499318, KF499319and KF499317 respectively.The toxicity tests on Aliivibrio fischeri showed that after 30 min of incubationEC50 for PNP was 9.7 mg/L. Degradation process has resulted in the removal oftoxic PNP from the medium, since degradation products didn’t have any toxiceffect on A. fischeri.