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Genetic Regulatory Mechanisms in the Synthesis of Proteins François Jacob and Jacques Monod. Journal of Molecular Biology (1961) 3: 318-356 Trish Barker: access.ncsa.uiuc.edu/CoverStories/lac / A geneti c switch

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  • Slide 1
  • Genetic Regulatory Mechanisms in the Synthesis of Proteins Franois Jacob and Jacques Monod. Journal of Molecular Biology (1961) 3: 318-356 Trish Barker: access.ncsa.uiuc.edu/CoverStories/lac/access.ncsa.uiuc.edu/CoverStories/lac/ A genetic switch
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  • bacteria Gene Synthesis Source: The Economist 06
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  • Genome Transplantation in Bacteria: Changing One Species to Another Science June 21,, Carole Lartigue 1, John I. Glass 1*, Nina Alperovich 1, Rembert Pieper 1, Prashanth P. Parmar 1, Clyde A. Hutchison III 1, Hamilton O. Smith 1, J. Craig Venter 1 Desired program Desired gene
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  • Issues 1.Cost & length of DNA synthesis 2.How to boot the system 3.How to design and build it
  • Slide 5
  • Synthetic Biology A new foundational discipline to deal with the coming technology An effort to design and build standardized, integrated biological systems
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  • 10 years time You can design new systems Build them quickly and cheaply What are you going to build?
  • Slide 7
  • Brown iGEM International Genetically Engineered Machines Synthetic Biology @ Brown
  • Slide 8
  • iGEM Video
  • Slide 9
  • Engineering biology with standard parts
  • Slide 10
  • Registry of Standard Biological Parts Parts Devices Systems
  • Slide 11
  • The Process Design Model Build
  • Slide 12
  • 7 Brown Undergraduates Biomedical Engineering Biology Biophysics Chemical Engineering Computational Biology
  • Slide 13
  • iGEM Faculty Tayhas PalmoreBiomedical Engineering Alex BrodskyMolecular Biology, Cell Biology and Biochemistry Karen HaberstrohBiomedical Engineering Mark JohnsonMolecular Biology, Cell Biology and Biochemistry Jeffrey MorganPharmacology, Physiology & Biotechnology. Nicola NerettiPhysics Ben RaphaelCenter for Computational Biology Sherief RedaElectrical Engineering Suzanne SindiApplied Math Jay TangPhysics David TarganPhysics Gary WesselMolecular Biology, Cell Biology and Biochemistry
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  • UTRA grant & departments Lab space in Multi Disciplinary Lab Equipment sponsorship Support
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  • Brown iGEM Lead-detector Tri-stable Switch Two projects being built with biological parts
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  • Lead Detector
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  • The Abstract System Fluorescent Protein Lead Promoter Amplifier
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  • So how will this system work in the cell?
  • Slide 19
  • Promoter (always on) Lead Receptor Message Receptor Lead Promoter Message Producer Message Promoter Message Producer GFP NO LEAD Transcription factors are constitutively made by the first promoter. These proteins are poised to activate the Lead Detector promoter and Message Receiver promoter upon addition of lead.
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  • Message Receptor Lead Promoter Message Producer Message Promoter Message Producer GFP + Fluorescent Protein Output Lead turns on Detector promoter Promoter (always on) Lead Receptor
  • Slide 21
  • Tri-Stable Switch
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  • Bistable Switch Here is how a bistable switch should work: Continuous production of A or B without additional inducer AB
  • Slide 23
  • Tristable Switch A two state switch is possible. We want a stable switch with three distinct inducible states. ABC
  • Slide 24
  • pTet LacI AraC pLac AraC TetR pAra TetR LacI Pathway A Pathway B Pathway C Tristable Switch Design
  • Slide 25
  • Uses for a Multi-stable Switch Tissue Engineering Drug Delivery Simple Circuits
  • Slide 26
  • Applications of synthetic biology
  • Slide 27
  • Programmed cell-cell communication Engineer sender and receiver cells Programmed pattern formation S. Basu, et al A synthetic multicellular system for programmed pattern formation Nature 2005, 434, 1130.
  • Slide 28
  • Programmed cell-cell communication applied to Stem Cells? Cell fate regulators Aim: engineer tissues from stem cells Approach: drive differentiation of stems cells down a specific pathway using engineered sender cells
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  • Metabolic Engineering hijacking a cell to produce antimalarial precursor D-K. Ro et al. Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 2006, 440, 940 Population at risk: 3.2 Billion Endemic countries: 107 # of infections annually: 300-500 Million # of deaths annually: 1-3 Million Cost per adult course: $2.40
  • Slide 30
  • Biofuels Challenge: petroleum production peaks in 10-30 yr, declines until resources are exhausted; 1 gal of gasoline and diesel = 20 lbs CO 2 (7 tons/vehicle/yr) Discover microbes capable of pretreatment and/or hydrolysis of lignocellulosic material Engineer (i.e., synthetic biology) microorganisms to convert the monomer products of deconstruction into fuels BP, Synthetic Genomics-JCVI, Amyris Biotechnologies-UCB
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  • Synthetic Biology Funding
  • Slide 32
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center
  • Slide 33
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center
  • Slide 34
  • Slide 35
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center
  • Slide 36
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center
  • Slide 37
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center Cal to be hub for study of alternate fuel Group headed by UC Berkeley wins $500 million grant from BP Rick DelVecchio, Mark Martin, Chronicle Staff Writers Thursday, February 1, 2007
  • Slide 38
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center Cal to be hub for study of alternate fuel Group headed by UC Berkeley wins $500 million grant from BP Rick DelVecchio, Mark Martin, Chronicle Staff Writers Thursday, February 1, 2007 By MATTHEW L. WALDMATTHEW L. WALD Published: June 26, 2007 WASHINGTON, June 25 The Energy Department is creating three bioenergy research centers to find new ways to turn plants into fuel. The three centers, which the department described as three start-up companies with $125 million each in capital, will be in Oak Ridge, Tenn.; Madison, Wis.; and near Berkeley, Calif. They will involve numerous universities, national laboratories and private companies. The goal of the centers, which are to be announced on Tuesday, is to bring new technologies to market within five years. The new approach supports President Bushs goal of reducing gasoline consumption by 20 percent in 10 years.
  • Slide 39
  • The Synthetic Biology Engineering Research Center (SynBERC) is a multi-institution research effort to lay the foundation for synthetic biology, which aims to design and assemble biological components into integrated systems to accomplish specific tasks Engineered biological systems have enormous potential to solve a wide range of problems in human health, industrial processes, and renewable energy and the environment. SynBERC brings together many of the pioneers of synthetic biology, including prominent biologists and engineers from world-class institutions, to work together to lay the foundation for this nascent field. Broader impacts Industry: As catalyzed by SynBERC, synthetic biology promises to transform the biotechnology, high-technology, pharmaceutical, and chemical industries, as well as suppliers of genetic tools and custom DNA synthesis companies through its relationships with industry in synthetic biology-related fields.industry Education and training: SynBERC will educate a new cadre of synthetic biologists and biological engineers capable of designing biological parts and useful biological systems. Finally, SynBERCs education program will provide general information on synthetic biology for the general public, in-depth offerings for public policy professionals, and motivational information on opportunities in higher education for K-12 students.SynBERCs education program Synthetic biology in the social context: A unique function of SynBERC is its examination of the emerging field of synthetic biology within a frame of human practices, with reciprocal emphasis on ways that economic, political, and cultural forces may condition the development of synthetic biology and on ways that synthetic biology may significantly inform human security, health, and welfare through the new objects that it brings into the world.human practices SynBERC is a NSF Engineering Research Center Cal to be hub for study of alternate fuel Group headed by UC Berkeley wins $500 million grant from BP Rick DelVecchio, Mark Martin, Chronicle Staff Writers Thursday, February 1, 2007 By MATTHEW L. WALDMATTHEW L. WALD Published: June 26, 2007 WASHINGTON, June 25 The Energy Department is creating three bioenergy research centers to find new ways to turn plants into fuel. The three centers, which the department described as three start-up companies with $125 million each in capital, will be in Oak Ridge, Tenn.; Madison, Wis.; and near Berkeley, Calif. They will involve numerous universities, national laboratories and private companies. The goal of the centers, which are to be announced on Tuesday, is to bring new technologies to market within five years. The new approach supports President Bushs goal of reducing gasoline consumption by 20 percent in 10 years. $43 Million Grant From Gates Foundation Brings Together Unique Collaboration For Antimalarial Drug Science DailyScience Daily BERKELEY A $42.6 million grant from the Bill & Melinda Gates Foundation to the Institute for OneWorld Health, the first nonprofit pharmaceutical company in the United States, will create a powerful new approach to developing a more affordable, accessible cure for malaria, which kills more than a million children each year.
  • Slide 40
  • Department of Health and Human Services Participating Organizations National Institutes of Health (NIH), (http://www.nih.gov)http://www.nih.gov Components of Participating Organizations National Heart, Lung, and Blood Institute (NHLBI) (http://www.nhlbi.nih.gov) National Cancer Institute (NCI), (http://www.nci.nih.gov) National Institute on Aging (NIA) (http://www.nia.nih.gov) National Institute of Biomedical Imaging and Bioengineering (NIBIB) (http://www.nibib.nih.gov) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (http://www.niddk.nih.gov)http://www.nhlbi.nih.govhttp://www.nci.nih.govhttp://www.nia.nih.govhttp://www.nibib.nih.govhttp://www.niddk.nih.gov Title: Bioengineering and Obesity (R01) Announcement Type New Update: The following update relating to this announcement has been issued: April 23, 2007April 23, 2007 - See Notice (NOT-HL-07-112) The purpose of this notice is to update the contact information. NOTICE: Applications submitted in response to this Funding Opportunity Announcement (FOA) for Federal assistance must be submitted electronically through Grants.gov (http://www.grants.gov) using the SF424 Research and Related (R&R) forms and the SF424 (R&R) Application Guidehttp://www.grants.gov APPLICATIONS MAY NOT BE SUBMITTED IN PAPER FORMAT. This FOA must be read in conjunction with the application guidelines included with this announcement in Grants.gov/Apply for Grants (hereafter called Grants.gov/Apply). Grants.gov/Apply for Grants A registration process is necessary before submission and applicants are highly encouraged to start the process at least four weeks prior to the grant submission date. See Section IV.Section IV Program Announcement (PA) Number: PA-07-354
  • Slide 41
  • Executive Summary Purpose This Funding Opportunity Announcement (FOA) issued by the National Heart, Lung, and Blood Institute, National Institutes of Health, solicits Research Project Grant (R01) applications from institutions/organizations that propose to solicit applications to develop and validate new and innovative engineering approaches to address clinical problems related to energy balance, intake, and expenditure. Novel sensors, devices, imaging, and other technologies, including technologies to detect biochemical markers of energy balance are expected to be developed and evaluated by collaborating engineers, physical scientists, mathematicians, and scientists from other relevant disciplines with expertise in obesity and nutrition. Mechanism of Support. This FOA will utilize the NIH Research Project Grant (R01) award mechanism and runs in parallel with an FOA of identical scientific scope, RFA-HL-07-007, that solicits applications under the R21 mechanism and FOA number(s) as appropriate.RFA-HL-07-007 Funds Available and Anticipated Number of Awards. Awards issued under this FOA are contingent upon the availability of funds and the submission of a sufficient number of meritorious applications. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size and duration of each award will also vary. The total amount awarded and the number of awards will depend upon the mechanism numbers, quality, duration, and costs of the applications received. Eligible Institutions/Organizations. Public/State Controlled Institution of Higher Education; Private Institution of Higher Education; Nonprofit with 501(c)(3) IRS Status (Other than Institution of Higher Education); Nonprofit without 501(c)(3) IRS Status (Other than Institution of Higher Education); Small Business; For-Profit Organization (Other than Small Business); State Government; U.S. Territory or Possession; Indian/Native American Tribal Government (Federally Recognized); Indian/Native American Tribal Government (Other than Federally Recognized); Indian/Native American Tribally Designated Organization; Non-domestic (non-U.S.) Entity (Foreign Organization); Hispanic-serving Institution; Historically Black Colleges and Universities (HBCUs); Tribally Controlled Colleges and Universities (TCCUs); Alaska Native and Native Hawaiian Serving Institutions; Regional Organization. Eligible Project Directors/Principal Investigators (PDs/PIs). Individuals with the skills, knowledge, and resources necessary to carry out the proposed research are invited to work with their institution/organization to develop an application for support. Individuals from underrepresented racial and ethnic groups as well as individuals with disabilities are always encouraged to apply for NIH support. Number of Applications. Applicants may submit more than one application, provided each application is scientifically distinct. Renewals and Resubmissions. Applications can be renewed by competing for additional project periods. Applicants may submit a resubmission application, but such application must include an Introduction addressing the previous peer review critique (Summary Statement).. Number of PDs/PIs. More than one PD/PI, or multiple PDs/PIs, may be designated on the application. Application Materials. See Section IV.1 for application materials.Section IV.1 General Information. For general information on SF424 (R&R) Application and Electronic Submission, see these Web sites: SF424 (R&R) Application and Electronic Submission Information: http://grants.nih.gov/grants/funding/424/index.htmhttp://grants.nih.gov/grants/funding/424/index.htm General information on Electronic Submission of Grant Applications: http://era.nih.gov/ElectronicReceipt/http://era.nih.gov/ElectronicReceipt/ Hearing Impaired. Telecommunications for the hearing impaired is available at: TTY 301-451-0088.
  • Slide 42
  • Executive Summary Purpose This Funding Opportunity Announcement (FOA) issued by the National Heart, Lung, and Blood Institute, National Institutes of Health, solicits Research Project Grant (R01) applications from institutions/organizations that propose to solicit applications to develop and validate new and innovative engineering approaches to address clinical problems related to energy balance, intake, and expenditure. Novel sensors, devices, imaging, and other technologies, including technologies to detect biochemical markers of energy balance are expected to be developed and evaluated by collaborating engineers, physical scientists, mathematicians, and scientists from other relevant disciplines with expertise in obesity and nutrition. Mechanism of Support. This FOA will utilize the NIH Research Project Grant (R01) award mechanism and runs in parallel with an FOA of identical scientific scope, RFA-HL-07-007, that solicits applications under the R21 mechanism and FOA number(s) as appropriate.RFA-HL-07-007 Funds Available and Anticipated Number of Awards. Awards issued under this FOA are contingent upon the availability of funds and the submission of a sufficient number of meritorious applications. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size and duration of each award will also vary. The total amount awarded and the number of awards will depend upon the mechanism numbers, quality, duration, and costs of the applications received. Eligible Institutions/Organizations. Public/State Controlled Institution of Higher Education; Private Institution of Higher Education; Nonprofit with 501(c)(3) IRS Status (Other than Institution of Higher Education); Nonprofit without 501(c)(3) IRS Status (Other than Institution of Higher Education); Small Business; For-Profit Organization (Other than Small Business); State Government; U.S. Territory or Possession; Indian/Native American Tribal Government (Federally Recognized); Indian/Native American Tribal Government (Other than Federally Recognized); Indian/Native American Tribally Designated Organization; Non-domestic (non-U.S.) Entity (Foreign Organization); Hispanic-serving Institution; Historically Black Colleges and Universities (HBCUs); Tribally Controlled Colleges and Universities (TCCUs); Alaska Native and Native Hawaiian Serving Institutions; Regional Organization. Eligible Project Directors/Principal Investigators (PDs/PIs). Individuals with the skills, knowledge, and resources necessary to carry out the proposed research are invited to work with their institution/organization to develop an application for support. Individuals from underrepresented racial and ethnic groups as well as individuals with disabilities are always encouraged to apply for NIH support. Number of Applications. Applicants may submit more than one application, provided each application is scientifically distinct. Renewals and Resubmissions. Applications can be renewed by competing for additional project periods. Applicants may submit a resubmission application, but such application must include an Introduction addressing the previous peer review critique (Summary Statement).. Number of PDs/PIs. More than one PD/PI, or multiple PDs/PIs, may be designated on the application. Application Materials. See Section IV.1 for application materials.Section IV.1 General Information. For general information on SF424 (R&R) Application and Electronic Submission, see these Web sites: SF424 (R&R) Application and Electronic Submission Information: http://grants.nih.gov/grants/funding/424/index.htmhttp://grants.nih.gov/grants/funding/424/index.htm General information on Electronic Submission of Grant Applications: http://era.nih.gov/ElectronicReceipt/http://era.nih.gov/ElectronicReceipt/ Hearing Impaired. Telecommunications for the hearing impaired is available at: TTY 301-451-0088.
  • Slide 43
  • Executive Summary Purpose This Funding Opportunity Announcement (FOA) issued by the National Heart, Lung, and Blood Institute, National Institutes of Health, solicits Research Project Grant (R01) applications from institutions/organizations that propose to solicit applications to develop and validate new and innovative engineering approaches to address clinical problems related to energy balance, intake, and expenditure. Novel sensors, devices, imaging, and other technologies, including technologies to detect biochemical markers of energy balance are expected to be developed and evaluated by collaborating engineers, physical scientists, mathematicians, and scientists from other relevant disciplines with expertise in obesity and nutrition. Mechanism of Support. This FOA will utilize the NIH Research Project Grant (R01) award mechanism and runs in parallel with an FOA of identical scientific scope, RFA-HL-07-007, that solicits applications under the R21 mechanism and FOA number(s) as appropriate.RFA-HL-07-007 Funds Available and Anticipated Number of Awards. Awards issued under this FOA are contingent upon the availability of funds and the submission of a sufficient number of meritorious applications. Because the nature and scope of the proposed research will vary from application to application, it is anticipated that the size and duration of each award will also vary. The total amount awarded and the number of awards will depend upon the mechanism numbers, quality, duration, and costs of the applications received. Eligible Institutions/Organizations. Public/State Controlled Institution of Higher Education; Private Institution of Higher Education; Nonprofit with 501(c)(3) IRS Status (Other than Institution of Higher Education); Nonprofit without 501(c)(3) IRS Status (Other than Institution of Higher Education); Small Business; For-Profit Organization (Other than Small Business); State Government; U.S. Territory or Possession; Indian/Native American Tribal Government (Federally Recognized); Indian/Native American Tribal Government (Other than Federally Recognized); Indian/Native American Tribally Designated Organization; Non-domestic (non-U.S.) Entity (Foreign Organization); Hispanic-serving Institution; Historically Black Colleges and Universities (HBCUs); Tribally Controlled Colleges and Universities (TCCUs); Alaska Native and Native Hawaiian Serving Institutions; Regional Organization. Eligible Project Directors/Principal Investigators (PDs/PIs). Individuals with the skills, knowledge, and resources necessary to carry out the proposed research are invited to work with their institution/organization to develop an application for support. Individuals from underrepresented racial and ethnic groups as well as individuals with disabilities are always encouraged to apply for NIH support. Number of Applications. Applicants may submit more than one application, provided each application is scientifically distinct. Renewals and Resubmissions. Applications can be renewed by competing for additional project periods. Applicants may submit a resubmission application, but such application must include an Introduction addressing the previous peer review critique (Summary Statement).. Number of PDs/PIs. More than one PD/PI, or multiple PDs/PIs, may be designated on the application. Application Materials. See Section IV.1 for application materials.Section IV.1 General Information. For general information on SF424 (R&R) Application and Electronic Submission, see these Web sites: SF424 (R&R) Application and Electronic Submission Information: http://grants.nih.gov/grants/funding/424/index.htmhttp://grants.nih.gov/grants/funding/424/index.htm General information on Electronic Submission of Grant Applications: http://era.nih.gov/ElectronicReceipt/http://era.nih.gov/ElectronicReceipt/ Hearing Impaired. Telecommunications for the hearing impaired is available at: TTY 301-451-0088. Recent faculty searches include: CalTech, MIT, Emory, UC Davis
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  • November 24, 2005
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  • Key people, institutes & companies BU: Jim Collins, Tim Gardner, Havard: George Church, Pam Silver Caltech: Francis Arnold, Michael Elowitz, Christina Smolke, MIT Drew Endy, Tom Knight, Alexander van Oudenaarden Princeton Ron Weiss, UC Berkeley Adam Arkin, Jay Keasling UC Berkeley Adam Arkin, Jay Keasling UCSF Wendell Lim Chris Voigt UT Austin Andy Ellington Edward Marcotte
  • Slide 47
  • Should Brown pursue synthetic biology as part of its academic plan?