Apostle COVID-19 Viral RNA

Isolation Automation System

Apostle’s response and solution

to help our community fight

COVID-19 together

2020 San Jose, California. All rights reserved.

E-mail : order@apostlebio.com

Address : 160 E Tasman Dr, San Jose, CA 95134

©2017-2020. Apostle, Inc. All rights reserved.

Website : http://apostlebio.com

Twitter: @ApostleInc LinkedIn:https://www.linkedin.com/

company/apostle-inc/

• High Quality

• Lower Cost

• Faster

• Safer

CONTENTS

Product Overview.……………………….......…..…01

Product Features ……....…………………......……02

Simple Isolation Workflow and Consistent

Results ………………………………….…….…….03

Stable and Reliable Isolation Results ………..…..04

Consistent Result between Manual and

Automated Isolation ….…………..…………..…..05

System is integrated with an internal UV

light for unit interior disinfection ..…………..…..06

Quality Assurance…………………………….........07

Order Information …………………………….........10

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1

Apostle , Inc. was founded in the

Silicon Valley with a mission to

building global leadership in biological

sample preparation and molecular

diagnostics, and continuously

providing original technology

breakthrough and services for public

health and life sciences.

COMPANY INTRODUCTION

The company has developed a

number of proprietary technologies

for cell-free nucleic acid preservation,

extraction and related sample

preparation methods and data

analysis. It has been recognized as a

member of Stanford University StartX

program in 2018, and has established a global exclusive partnership with

Beckman Coulter on one of its products - Apostle MiniMax® cfDNA Isolation

Kit in 2019.

Our Response and Solution to fight COVID19

Apostle is committed to providing our cutting edge technologies to help flight

COVID19. Our automated viral RNA extraction system, composed of Apostle

MagTouchTM Nucleic Acid Isolation Automation Platform, coupled with

Apostle MiniGenomicsTM Viral TotalNA Isolaion Kit, provides high quality,

lower cost, faster, safer sample preparation for COVID19 tests.

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Apostle COVID-19 Viral RNA

Isolation Automation System

01 02

MagTouch Automated

Nucleic Acid Isolation

System

MiniGenomics Viral

TotalNA Isolation Fast Kit

+

Apostle MiniGenomicsTM Viral Total NA Isolation Fast kit

1. Product Description

For fast extraction and purification of viral nucleic acids from

various kinds of biological samples collected in multiple transport

media.

2. Product features

Novel and original magnetic nanoparticle technology for

nucleic acids isolation

Various sample types: swabs, saliva, blood

Optimized standardized protocol for viral total NA isolation

in 30 min without heating

Compatible with multiple high-throughput automated

nucleic acid extraction platforms

Configuration: 96 preps (A200619-96),

384 preps (A200619-384),

1000 preps (A200619-1000)

High Quality, Lower Cost, Faster, Safer

High quality: Equal or better results compared with market leading products.

Lower cost: Guaranteed 30% - 50% less cost than other products on market.

Faster: One button start; Run time is approximately 40 minutes per 96 samples.

Safer: Integrated with a UV light for unit interior disinfection.

• High Quality

• Lower Cost

• Faster

• Safer

03 04

Kit Avg Ct

Viral Total NA

Isolation Kit

Apostle Viral Total NA Isolation Kit 31.65

Alternative product * 32.34

A widely used brand.

3. Simple isolation workflow and consistent results:

Typical magnetic bead-based workflow

40 mins process time

No heating required

Compatible with downstream RT-PCR detection

Consistent results with major brands

RT-PCR results for internal reference target gene RPP30

* No heating required

Kit Avg Ct

Viral Total NA

Isolation Kit

Apostle Viral Total NA Isolation Kit 27.09

Alternative product * 30.44

RT-PCR results for spike-in target gene dnaN from bacteria Sample lysis and RNA binding in

one step

Wash with wash buffer

Wash with 80% EtOH x2

Elution

4. Stable and reliable isolation results：

05

5. Consistent Result between Manual and Automated Isolation

Viral RNA isolation by manual and MagTouch is consistent.

Protocol:

Throat swab samples collected in PBS

Total NA isolation by Apostle Viral Total NA Isolation Fast Kit

Manual isolation and automation isolation on MagTouch 1000 (96

throughput) in parallel

RT-PCR for internal reference target gene detection or for spike-in bacteria

RNA.

Methods Avg Ct

Apostle Viral Total NA Isolation Kit

Manual Isolation 31.65

Automation Isolation 31.85

RT-PCR results for internal reference target gene RPP30

RT-PCR results for spike-in target gene dnaN from bacteria

Methods Avg Ct

Apostle Viral Total NA Isolation Kit

Manual Isolation 27.09

Automation Isolation 26.70

06

6. System is integrated with an internal UV light for unit

interior disinfection

We thank the COVID-19 fighters for battling with this highly contagious

virus. We understand the importance of working in a clean environment that

can be conveniently disinfected from time to time.

System is integrated with an internal UV light that can be conveniently

turned on between runs for unit interior disinfection.

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Other recognitions

Certificates and Licenses

• American Board of Medical Genetics and Genomics (ABMGG)

• New York State Department of Health Certificate of Qualification for Laboratory

Director in Genetic Testing and Oncology

• California State Department of Health License of Clinical Genetic Molecular Biologist

• Apostle Inc is a selected member of the BioSpace Top 20 Life Science Startups in January

2018.

• Apostle Inc is a selected member of the Stanford University StartX program in April 2018.

• Apostle Inc enters a liquid biopsy partnership with Beckman Coulter Life Sciences in February

2019.

• Apostle Inc obtains ISO13485:2016 & ISO9001:2015 certification in April 2020.

• Apostle MiniMax is a featured product in Science, New Products section in May 2019.

• Apostle Inc is selected to deliver a speech at the Precision Medicine World Conference in

January 2020.

• Apostle Inc is selected to deliver a speech at the 5th Liquid Biopsy Summit in June 2020.

Apostle offers cutting-edge innovations and exceptional products to meet the specific

needs for our clients. Our products are offered by Apostle’s team of top chemists,

scientists, bioinformaticians, and geneticists, working with a certified quality assurance

system.

Apostle has received the Certificates of Conformance to certify that: Quality

Management System of Apostle Inc. has been assessed by ABS Quality Evaluations,

Inc. and found to be in conformance with the requirements set forth by: ISO13485:2016,

and ISO9001:2015.

Manufacturing Certification

ISO 13485:2016

ISO 9001:2015

In addition, Apostle has board-certified clinical molecular geneticist and lab director

holding certificates and licenses from American Board of Medical Genetics and

Genomics (ABMGG), State of California, State of New York, and diploma from

American College of Medical Genetics (ACMG), and Harvard Medical School Genetics

Training Program.

7. Quality Assurance

8. Order Information

09

Order Information

Email: order@apostlebio.com

Website: http://apostlebio.com

Email：info@apostlebio.com

Order Information

Email: order@apostlebio.com

Publications

Apostle technologies have been discussed or cited in the following publications:

1. Cell-Free DNA Isolation Kit. Science. 17 May 2019:Vol. 364, Issue 6441, pp. 696. DOI: 10.1126/science.364.6441.696-a. (Featured in New Products section)

2. High-resolution DNA size enrichment using a magnetic nano-platform and application in non-invasive prenatal testing. Zhang et al. Analyst. 2020. In press.

3. G3viz: an R package to interactively visualize genetic mutation data using a lollipop-diagram. Xin G, Bo Z, Wenqi Z, et al. Bioinformatics. 2020; 36(3):928–929,

4. Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples. Niccum B., Heath C., Saunders L., Hur A.，Patel A. Association for Molecular Pathology (AMP). (abstract #ST103). Baltimore, MD. November 7-9, 2019

5. Dynamics of Plasma EGFR T790M Mutation in Advanced NSCLC: A Multicenter Study. Yang et al. Targeted Oncology. 2019;14:719-728. Published: 06 November 2019.

6. Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples. （n=8） Niccum B., Saunders L., Hur A.，Patel A. The American Society of Human Genetics (ASHG). (abstract #1766). Houston, TX. Oct 15, 2019

7. Comparison between mutation profiles of paired whole blood and cfDNA samples. Patel A., Saunders L., Hur A. The American Society of Human Genetics (ASHG). (abstract #1767). Houston, TX. Oct 15, 2019

8. Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMaxTM High Efficiency cfDNA Isolation kit—comparison of fully automated, semi-automated and manual workflow processing. Brittany Niccum, PhD., Randy Pares and Antonia Hur. Beckman Coulter Life Sciences. Application Note. Sept 2019.

9. A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMaxTM on the KingFisherTM Technology. Brittany Niccum, PhD. Beckman Coulter Life Sciences. Application Note. 2019.

10. Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples. (n=3) Saunders L and Patel A. American Association for Cancer Research (AACR). (abstract #2233). Atlanta, GA. April 2, 2019

11. A new Scalable and automatable method for the extration of cfDNA. Saunders LP, Hur A, Niccum B, and Patel A. Advances in Genome Biology and Technology (AGBT). (abstract #419). Marco Island, FL. Feb 28, 2019

12. cfDNA Extraction from Plasma for Liquid Biopsy: Apostle MiniMaxTM High Efficiency cfDNA Isolation Kit. Beckman Coulter Life Sciences, Data Sheet. 2019.

Apostle team’s track record of publications (selected):

1. Ge D*, Fellay J*, Thompson AJ*, Simon JS*, Shianna KV, Urban TJ, Heinzen EL, Qiu P, Bertelsen AH,

Muir AJ, Sulkowski M, McHutchison JG, Goldstein DB. Genetic variation in IL28B predicts hepatitis C

treatment-induced viral clearance. Nature 2009:461, 399-401.

2. Bo Zhang, Benjamin A. Pinsky, Jeyarama S. Ananta, Su Zhao, Shylaja Arulkumar, Hao Wan, Malaya K.

Sahoo, Janaki Abeynayake, Jesse J. Waggoner, Clay Hopes, Meijie Tang and Hongjie Dai. Diagnosis of

Zika virus infection on a nanotechnology platform. Nature Medicine, 23, 548, 2017.

3. Zeng WQ, Al-Yamani E, Acierno JS Jr, Slaugenhaupt S, Gillis T, MacDonald ME, Ozand PT, Gusella JF.

Biotin-responsive basal ganglia disease maps to 2q36.3 and is due to mutations in SLC19A3. Am J Hum

Genet. 2005 Jul;77(1):16-26

4. X Guo, A Bernard, DA Orlando, SB Haase, & AJ Hartemink. (2013). Branching process deconvolution

algorithm reveals a detailed cell-cycle transcription program. PNAS, 110, E968-E977.

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Item Cat # Unit Size

Apostle MagTouch Nucleic Acids Extraction Automation System

A190424 One MagTouch instrument, 96 well

Reagents and Consumables, Ordered in Package

Apostle MiniGenomics Viral Total NA Isolation Kit with Plates & Tip Comb Included

A200619P-384 One Kit, 384 (96X4) reactions, with plates and tip combs

A200619P-1536 One Kit, 1536 (96X16) reactions, with plates and tip combs

Reagents and Consumables, Ordered Separately

Apostle MiniGenomics Viral Total NA Isolation Kit (Not Including Plates & Tip Comb)

A200619-96 One Kit, 96 reactions

A200619-384 One Kit, 384 reactions

A200619-1000 One Kit, 1000 reactions

Apostle MagTouch 96 deep well plate

A191212-50 Fifty deep well plates, 96 well each

Apostle MagTouch 96 tip comb A191214-100 One hundred tip combs, 96 well each