an introduction to the sydney university proteome research unit
DESCRIPTION
An Introduction to the Sydney University Proteome Research Unit. Dr Ben Crossett. The History…. Opened in July 2004 with $2m MNRF grant & USyd funding Centre of excellence for proteomics Level 2, Building G08 People come from: SMB, SoBS, Medicine, VetSci, Agriculture, - PowerPoint PPT PresentationTRANSCRIPT
An Introduction to the An Introduction to the Sydney University Sydney University Proteome Research UnitProteome Research Unit
Dr Ben CrossettDr Ben Crossett
The History… • Opened in July 2004 with $2m
MNRF grant & USyd funding• Centre of excellence for
proteomics• Level 2, Building G08• People come from: SMB, SoBS,
Medicine, VetSci, Agriculture, • ...as well as: MQ, UNWS, UWS,
UTS, Brisbane, UK, USA, Germany etc...
To gel or...
...or not to gel
The Results…
• Whole protein mass• Identify proteins• Labelling QC• Changes in protein
abundance, e.g. DIGE or iTRAQ
• Mapping post-translational modifications
• MS ‘western blots’
2000.0 7600.2 13200.4 18800.6 24400.8 30001.0
Mass (m/z)
1.2E+4
0
10
20
30
40
50
60
70
80
90
100
% In
ten
sity
Voyager Spec #1[BP = 23504.3, 12073]
23505.27
23452.36
23401.56
23585.13
23622.9023659.68
23700.26
23744.51
11742.36
23319.85
2014.3623799.20
2280.91
3014.85
2466.6411846.38
2362.002856.22
23162.722979.573832.54 11936.27
22607.404491.42
5796.63 25204.0117286.42
Accessing SUPRU • Contract research: samples
analysed for fixed fee • Research hotel: come and work
in SUPRU • Collaborative research projects• Short courses &
under/postgraduate lectures
• Contact us at:sydney.edu.au/[email protected]