An Introduction to the An Introduction to the Sydney University Sydney University Proteome Research UnitProteome Research Unit

Dr Ben CrossettDr Ben Crossett

The History… • Opened in July 2004 with $2m

MNRF grant & USyd funding• Centre of excellence for

proteomics• Level 2, Building G08• People come from: SMB, SoBS,

Medicine, VetSci, Agriculture, • ...as well as: MQ, UNWS, UWS,

UTS, Brisbane, UK, USA, Germany etc...

To gel or...

...or not to gel

The Results…

• Whole protein mass• Identify proteins• Labelling QC• Changes in protein

abundance, e.g. DIGE or iTRAQ

• Mapping post-translational modifications

• MS ‘western blots’

2000.0 7600.2 13200.4 18800.6 24400.8 30001.0

Mass (m/z)

1.2E+4

0

10

20

30

40

50

60

70

80

90

100

% In

ten

sity

Voyager Spec #1[BP = 23504.3, 12073]

23505.27

23452.36

23401.56

23585.13

23622.9023659.68

23700.26

23744.51

11742.36

23319.85

2014.3623799.20

2280.91

3014.85

2466.6411846.38

2362.002856.22

23162.722979.573832.54 11936.27

22607.404491.42

5796.63 25204.0117286.42

Accessing SUPRU • Contract research: samples

analysed for fixed fee • Research hotel: come and work

in SUPRU • Collaborative research projects• Short courses &

under/postgraduate lectures

• Contact us at:sydney.edu.au/proteomicsben.crossett@sydney.edu.au