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Date Released: August 23, 2006

Republic of the Philippines

Department of Agriculture

BUREAU OF PLANT INDUSTRY

PLANT VARIETY PROTECTION OFFICE

Volume 2 Issue1

Plant Variety Gazette - Volume 2 Issue 1

Inside this issue

Page Applications: Corn

BIOSEED 9909……………………………………………………….. BIOSEED 9888…………………………………………………….…. CZ192………………………………………………………………… CZ892-nm……………………………………………………………. CZ280………………………………………………………………… CY888-nm……………………………………………………………. PCI001………………………………………………………………… 92P310………………………………………………………………… DE907………………………………………………………………… 92C112C……………………………………………………………… 91P274C……………………………………………………………… 01R054……………………………………………………………….. CZ076-nm………………………………………………………….… CZ172-nm………………………………………………………….… BIOSEED 9899…………………………………………………….…

1 2 4 5 7 8

10 12 13 14 15 16 18 20 21

Rice Arize H64…………………………………………………………….

17

Bitter Gourd Bonito F1……………………………………………………………..

11

Notice of Withdrawal of Application for Certificate of Plant Variety Protection……………………………………………………………………..

23

Test Guidelines Corn…………………………………………………………………... 24 Rice……………………………………………………………………. 40 Vegetable Legumes (Cowpea, Yardlong Beans, Bush Sitao)……………………………

75

Eggplant……………………………………………………………... 93 Watermelon……………………………………………………….… 107 Onion and Shallot…………………………………………………... 127 Tomato……………………………………………………………..… 147


1

Application Number : 06-0040

Filing Date : March 28, 2006

Applicant : BIOSEED Research Philippines, Inc. (BRPI) National Highway, Katangawan, General Santos City 9500 Philippines

Crop : Corn

Proposed Denomination

: 9909 is a modified single cross hybrid

Description of the variety :

a) Leaf Anthocyanin

Medium (score 5) b) Foliage

Light green erect leaves (score 1) c) Ears

Medium sized uniform ears completely filled up to the tip d) Silk

Light purple e) Anther color

Light brown with light coloration at glume base f) Grain texture

12-14 kernel rows (some time 16 kernel row also); flat grains semi flint texture g) Cob color

White (score 1) h) Prolific Behavior

If hybrid is planted spaciously then it bears double ears per plant i) Disease reaction

Very good tolerance for Stalk and Ear rots Filipino Translation Mga Katangian ng mga Barayti

Ang BIO 9909 ay isang modified single cross hybrid;

a) Anthocyanin sa dahon

bahagyang matingkad (score 5) b) Kadahunan

mapusyaw na berde at tuwid ang mga dahon (score 1) c) Anther

mapusyaw ng tsokolate at may bahagyang kulay sa glume base d) Buhok ng puso

mapusyaw na ube e) Prolific Behavior

nagdadalawang puso bawat halaman kapag malapad ang agwat ng tanim f) Puso

Bahagyang kalakihan, halos magkakasinlaki ang mga puso, may 12-14 na hanay ng butil (16 pa kung minsan).

Applications


2

g) Kulay at kalidad ng butil

lapad ng mga butil at semi-flint. h) Kulay ng busil/busal

puti (score 1) i) Reaksyon sa mga sakit at peste

may higit ng mabuting tolerance sa Stalk at Ear rots.

--00--




Crop : Corn


: BIOSEED 9888


a) Hybrid Type

9888 is a 3 - way cross hybrid b) Leaf Anthocyanin

light (score 5) c) Foliage

Dark green slightly curved leaves (score 3) d) Ears

Big ear sized fully covered with husk leaves e) Grain color and Texture

BIO 9888 has bold orange yellow flint kernel f) Silk

Silk emerge as green and once expose to sunlight it will change into slightly purple color

Applicatons


3

g) Anther color

Brown h) Grain texture

12-14 kernel rows; orange yellow flint texture i) Cob color

white (score 1) j) Prolific Behavior

mostly BIO 9888 produce single ear per plant k) Disease reaction

good tolerance for disease and insect pest. Filipino Translation Mga Katangian ng mga Barayti

Ang BIO 9888 ay isang 3-way cross hybrid;


maputla (score 5) b) Kadahunan

Matingkad na berde na may bahagyang kurbadong mga dahon (score 3). c) Anther

Kulay tsokolate d) Buhok ng puso

lumalabas sa puso na kulay berde ngunit nagiging mapusyaw na ube kapag nasikatan ng araw.

e) Prolificacy

ang BIO 9888 ay may isang puso kada isang halaman. f) Puso

malalaki ang puso na may 12-14 na hanay ng butil at kumpletong natatakpan ng dahong-balat.

g) Kulay at kalidad ng butyl

matingkad na magkahalong dilaw at dalandan na flint. h) Kulay ng busil/busal


may mabuting tolerance

Applications


4




Crop : Corn


: CZ192


a) Hybrid Type

CZ192 is a inbred line b) Leaf Anthocyanin

Medium (score 3) c) Foliage

Dark green slightly curved or semi erect leaves d) Ears

Medium and uniform ears size e) Silk

Dark purple f) Anther color

Medium anthocyanin coloration of anthers g) Grain texture

12 kernel rows sometimes 14 kernel rows h) Cob color

white (score 1) i) Prolific Behavior

develop the second ear in high field management j) Disease reaction

good tolerance for foliar disease Filipino Translation Mga Katangian ng mga Barayti

Ang CZ192 ay isang inbred line.


mapusyaw na kulay ( score 3) b) Kadahunan

matingkad na berde at bahagyang kurbadong mga dahon c) Anther

mapusyaw na kulay ng anthocyanin sa mga anthers d) Buhok ng puso

matingkad na ube e) Prolificacy

nagdadalawa ang puso kapag maganda ang pag- aalaga

Applications


5

f) Puso

Bahagyang kalakihan at halos magkakasinlaki g) Kulay at kalidad ng butyl

12 hanggang 14 kernel na hanay ng butil. h) Kulay ng busil/busal


may mabuting tolerance sa mga sakit ng dahon

--00--




Crop : Corn


: CZ892-nm


a) Hybrid Type

CZ892-nm is an inbred line b) Leaf Anthocyanin

Anthocyanin coloration of the sheath is medium (score 5) c) Foliage

Light green erect leaves (score 1) d) Ears

Small conical e) Silk

Purple silk (score 7-9) f) Grain texture

Yellow semi flint

Applications


6

g) Cob color

White h) Prolific Behavior

good percentage of producing two ears per plant i) Disease reaction

good tolerance for stalk rot Filipino Translation Mga Katangian ng mga Barayti

Ang CZ892-nm ay isang inbred line.

a) Anthocyanin

bahagyang matingkad na anthocyanin sa leafsheath (score 5) b) Kadahunan

mapusyaw na berde at tuwid na mga dahon (score 1). c) Anther

bahagyang matingkad na kulay ube ang anthers ngunit walang kulay ang glume base

d) Buhok ng puso

Matingkad na ube na sakop lahat ang buhok (score 7-9) e) Prolificacy

maraming halaman ang nagdadalawang puso bawat halaman. f) Puso

maliliit na conical g) Kulay at kalidad ng butil

Dilaw na semi- flint h) Kulay ng busil/busal


mabuting tolerance sa stalk rot.

Applications


7




Crop : Corn


: CZ280


a) Hybrid Type

CZ280 is an inbred line b) Foliage

Dark green erect leaves slightly curve (score 3) c) Ears

Long ears tapered on tip and some has extended growth on tip d) Silk

Medium purple (score 7) e) Anther color

Dark brown anther color and coloration at glume base is absent f) Grain texture

Yellow orange flint g) Cob color

White h) Prolific Behavior

no prolification always single ear per plant i) Anthocyanin on brace roots

week (score 3) j) Disease reaction

excellent tolerance for stalk rot Filipino Translation Mga Katangian ng mga Barayti


a) Kadahunan

matingkad na berde at bahagyang kurbadong mga dahon (score 3). b) Bulaklak

bahagyang kalakihang bulaklak na may mahabang katawan at malilit na sanga

c) Anther

Matingkad na tsokolate ang kulay ng anther at walang kulay ang glume base d) Buhok sa puso

bahagyang matingkad na ube (score 7) e) Prolificacy

palaging isang puso bawat halaman

Applications


8

f) Puso

Mahahabang puso na makitid sa dulo at may g) Kulay at kalidad ng butil

magkahalong dilaw at dalandan na flint h) Kulay ng busil/busal

puti (score 1) i) Anthocyanin sa brace roots

mapusyaw na kulay (score 3) j) Reaksyon sa mga sakit at peste

maasahang tolerance sa stalk rot.

--00--




Crop : Corn


: CY888-nm


a) Hybrid Type

CY888-nm is an inbred line b) Leaf Anthocyanin

absent (score 1) c) Foliage

green leaves strongly curved (score 7) d) Ears

Cylindrical ear with good diameter

e) Silk

whitish green f) Anther color

Brown g) Grain texture

Applications


9

orange flint h) Cob color

white (score 1) i) Prolific Behavior

Single ear per plant j) Disease reaction

excellent tolerance for stalk rot

Filipino Translation Mga Katangian ng mga Barayti

Ang CY888-nm ay isang inbred line


walang makikitang kulay (score 1) b) Kadahunan

berde at matindi ang kurbada ng mga dahon (score 7) c) Bulaklak (Tassel )

kaunti ngunit mahahabang sanga ng bulaklak d) Anther

kulay tsokolate e) Buhok ng puso

berdeng may halong puti f) Prolificacy

isang puso bawat halaman g) Puso

hugis cylindrical na may katabaan h) Kulay at kalidad ng butil

kulay dalandan na flint i) Kulay ng busil/busal

puti (score 1) j) Reaksyon sa mga sakit at peste

may mabuting tolerance sa mga sakit ng dahon ngunit mahina laban sa stress

Applications


10




Crop : Corn


: PCI001


a) Hybrid Type

PCI001 is an inbred line b) Foliage

Light green erect leaves (score 1) c) Ears

Medium ear size d) Silk

Light green e) Anther color

Light green with light coloration at glume base f) Grain texture

Yellow orange flint g) Cob color

white h) Prolific Behavior

tendency to develop second ear i) Anthocyanin on brace roots

week (score 3) j) Disease reaction

excellent tolerance for stalk rot Filipino Translation Mga Katangian ng mga Barayti

Ang PCI001 ay isang inbred line.

a) Kadahunan

mapusyaw na berde at tuwid na mga dahon (score 1). b) Bulaklak

tuwid ang mga sanga ( score 1) c) Anther

mapusyaw na berde at may mapusyaw ding kulay ang glume base d) Buhok ng puso

mapusyaw na berde (score 1) e) Prolificacy

may kakayahang magbigay ng ikalawang puso

Applications


11

f) Puso

May kalakihan g) Kulay at kalidad ng butil

magkahalong dilaw at dalandan na flint h) Kulay ng busil/busal

puti (score 1) i) Anthocyanin sa brace roots

mapusyaw na kulay (score 3) j) Reaksyon sa mga sakit at peste

maasahang tolerance sa stalk rot.

--00--


Filing Date : June 7, 2006

Applicant : East-West Seed Company Inc. Km 54 Cagayan Valley Rd., Bgy. Sampaloc, San Rafael 3008, Bulacan

Crop : Bittergourd, Ampalaya


: Bonito F1


“Bonito F1” is a very vigourous and high-yielding native type bittergourd hybrid. The closest variety is the open-pollinated farmers variety “Ilocos native(OP)”. However, “Bonito F1 consistently gave higher yield than “Ilocos native(OP)”. Under normal and similar growing conditions where we compared the two varieties, “Bonito F1” gave and average of 44 fruits per plant while “Ilocos native(OP)” has an average of 20 fruits per plant. Fruits of “Bonito F1” are slightly longer with an average length of 11 cm compared to 9cm of “Ilocos native(OP)”. Bonito F1 fruits are more uniform in color (medium green) and in shape (elongated) as compared to “Ilocos native(OP)” which is variable in color (lightgreen to medium green) and shape (high round to elongate)

Applications


12

Filipino Translation Mga Katangian ng Binhi Ang Bonito F1 ay isang uri ng native ampalaya hybrid na may napakagandang bulas ng halaman at nagbibigay ng napakataas na ani. Maikukumpara ito sa open pollinated na variety na ginagamit ng mga magkakasala na kilala bilang “Ilocos native OP”. Ngunit ang Bonito F1 ay naiiba sa “Ilocos native OP” dahil ito ay may mas makapal na usbong at nagbibigay ng higit na mas mataas na ani kumpara sa “Ilocos native OP”. Sa parehong kondisyon ng pagtatanim kung saan ikinumpara ang dalawang uri, ang Bonito F1 ay nagbibigay ng mahigit kumulang na 44 bunga kada halaman samantalang ang “Ilocos native OP” ay nagbibigay lamang na mahigit kumulang na 20 bunga kada halaman. Ang bunga ng Bonito F1 ay mahaba ng kaunti at may sukat ng labing isa (11) sentimetro kumpara sa siyam (9) na sentimetrong haba ng “Ilocos native OP”. Kapansin pansin rin sa mga bunga ng Bonito F1 ang pare-parehong berdeng kulay at pahabang hugis kumpara sa “Ilocos native OP” na iba-iba ang berdeng kulay at hugis (bilugan hangang pahaba).

--00--



Applicant : Tropical Seed Genetics Sciences Incorporation Macnit, Maladag, Malungon, Sarangani Province

Crop : Corn


: 92P310

Varietal Characteristics :

92P310 is a tall corn inbred line with long droopy light green leaves. It has sparse

tassel with few tassel branches and purple glume. It has prolific tendency. Stalks are big

predominantly green but with shades of purple. It has high density yellow orange flint kernels

of medium size. Under Malandag condition days to silk is 58 days and days to pollen shed is

60 days. Silk is predominantly cream with light purpling of the tip. Root system excellent with

prominent adventitious roots. Husk cover excellent. Produces side cobs. Excellent combine

Applications


13

Filipino Translation Mga Katangian ng Binhi

92P310 ay matangkad na klase ng mais na inbred line na may mahabang nanlupaypay na dahon na kulay hilaw na berde. Ito rin ay may konting tassel na pinagbubuklod rin ng konting sanga at kulay lila ang glume nito. May kakayahan din itong magbunga ng marami. Ang tangkay nito ay malalaki na pinatingkad ng kulay nitong berde na may guhit na lila. Nagtataglay din ito ng marami at magkakadikit-dikit na kulay yellow orange flint kernels na katamtaman ang laki. Sa Malandag na kalagayan nito lumalabas ang silk nito limangput walung araw at animnapung araw ang paglabas ng pollen nito. Ang silk nito ay kulay krema na may halong lila sa dulo nito. Ang mga ugat nito ay napakagaling dahil sa taglay nitong prominenting adventitious roots. Ang balat ng bunga nito ay maganda at kaaya-aya. Nagbibigay din ito ing side cobs. At may magandang kakayahan as combiner.

--00--




Crop : Corn

Proposed Denomination : DE907

Varietal Characteristics : DE907 is a corn inbred line with semi-erect light green leaves. It has medium plant height and medium size stalks of good quality. It has medium size hard yellow orange flint kernels of excellent quality. Husk cover is excellent. It is good male having many tassel branches with good pollen yield. Silk and tassel are purple. It has prolific tendency with prefix de meaning double ear. It has excellent root system. The line sheds pollen is 53 days and silks in 54 days under Malandag conditions. It is an excellent combiner.

Applications


14


Ang DE907 ay isang mais na inbred line na may medyo nakatayo na dahon na kulay hilaw na berde. Ito ay may katamtaman ang taas at katamtaman ang sukat ng mga tangkay nito pero maganda ang kalidad. Nagtataglay din ito ng matigas na butyl na may katamtaman ang sukat at kulay yellow orange flint kernels na may magandang katangian. Maganda din ang balat ng bunga nito. Dahil sa taglay nitong maraming tassel at magandang pollen mainam itong gawing lalaki sa breeding. Ang silk at tassel ay kulay lila. May kapasidad na magbigay ng tig-dalawang bunga. Maganda ang mga ugat nito. Lumalabas ang pollen nito limangput tatlong arawat ang silk nito ay limangput apat na araw sa ilalaim ng Malandag na kalagayan. Ito ay magandang combiner.

--00--




Crop : Corn

Proposed Denomination : 92C112C

Varietal Characteristics : 92C112C is a corn inbred line with narrow semi-erect light green, thick waxy leaves. It has big stalks of excellent quality. It has short compact tassel that has good pollen yield. It has medium size ears with excellent tip filling bearing hard flint orange kernels with 16 to 18 kernel rows per ear. Husk cover is short but acceptable. Stay green quality is excellent; Leaves remain green at harvest. It‟s a late line-days to shed is 62 days and days to silk is 60 days under Malandag, Malungon, Sarangani Province conditions.

Applications


15


Ang 92C112C ay isang mais na inbred line na may makitid, medyo nakatayo, makapal, at makintab na dahon na kulay hilaw na berde. Malalaki ang tangkay nito na maganda din ang kalidad. Ito ay may maikli at magkadikit-dikit na sanga ng tassel na nagbibigay ng magandang pollen. Katamtaman lang ang laki ng bunga nito. Matitigas at kulay dalandann ang butyl nito (flint kernels) na may labinanim hanggang labinwalong hanay ng butyl sa bawat bunga. Ang balat ng binga ay maikli. Nananatiling kulay berde ang mag dahon nito kapag inaani. Ito ay isang uri ng line na huli kung mahinog. Lumalabas ang pollen animnaput dalawang araw at ang silk ay animnapung araw sa Malandag na kalagayan.

--00--




Crop : Corn

Proposed Denomination : 91P274C

Varietal Characteristics : 91P274C is a corn inbred line of medium plant height with semi-erect light green leaves. It has big tassel with good pollen yield. Silks and tassel color are predominantly light purple. It has short ears but with big diameter having 18 kernel rows. It has deep orange hard flint kernels of excellent quality. Husk cover is good. Stay green excellent. Fair for roots. Days to silk is 59 days and days to shed is 60 days under Malandag, Malungon, Sarangani Province conditions.

Applications


16


Ang 91P274C ay isang mais na inbred line na may katamtaman ang taas at medyo nakatayo na dahon na kulay berde. Malalaki ang mga tassel nito na nagbibigay ng magandang pollen. Ang silk at tassel ay kulay hilaw na lila. Maiikli ang bunga pero malaki ang diyametro na nagtataglay ng labinwalong hanay. May matigas at magandang uri ng flint kernels ito kulay dalandan (deep orange). Maganda nag balat ng biunga nito. Nanatiling berde ang kulay ng kabuuang tanim na ito kahit malapit na itong aanihin. Kainaman ang ugat nito. Lumalabas ang silk nito limamput siyam na araw sa MAlandag na Kalagayan.

--00--




Crop : Corn

Proposed Denomination : 01R054

Varietal Characteristics : 01R054 is a medium tall corn inbred line with semi droopy leaves. It has excellent root system with very prominent adventitious roots. It has medium size cylindrical ears on good husk cover. It has medium size yellow orange flint kernels. It has very big tassels with very high pollen yield. Weakness is early lower leaf drying due to leaf blight infection. It is an excellent combiner.

Applications


17


01R054 ay isang mais na inbred line na may katamtaman ang taas. Ang mga dahon ay medyo nanlupaypay. Ang mga ugat nito ay maganda na may prominenteng adventitious na ugat (prominent adventitious roots). Ang bunga nito ay may katamtaman ang laki na may magandang balat.Ito ay may katamtaman na butil (flint kernels) na kulay yellow orange. And tassel ay malaki at maraming pollen. Ang dahon sa ibabang parte ay mapapansin na madaling matuyo dala ng sakit na leaf blight. Kaaya-ayang combiner.

--00--



Applicant : BAYER Crop Science Incorporation 3/F Bayer House, Canlubang Industrial Estate, Canlubang, Laguna, Philippines

Crop : Rice

Proposed Denomination : Arize H64

Varietal Characteristics :

Arize H64 hybrid rice, under favorable condition, has potential yield of 7.5 tons per hectare. It has a maturity range of 95 to 112 days. Approximate plant height is 116 cm. It has the following grain qualities: 25.49% amylose, G.T. Score of 5.1 I/L/HI, 67.8 Milling Recovery (Grade 1), 49.5% Headrice (Grade 1). It has a better sensory quality than IR 64, PSB Rc72H and PSB Rc18.

Arize H64 shows intermediate resistance to Rice Blast and Sheath Blight and shows low incidence of yellow and white stemborer infestation.

Applications


18

Filipino Translation Mga Katangian ng Binhi Ang Arize H64 hybrid rice ay may potensyal na ani na 7.5 tonelada bawat ektarya kapag mainam ang kondisyon. Maari nang anihin ito sa loob ng 95 hanggang 112 araw pagkatapos ipunla. Tinatantiyang nasa 116 sentimetro ang taas nito. Ang mga sumusunod ay ang kalidadng butil. 25.49% amylase, G.T. Score na 5.1 I/L/HI, 67.8%, Milling Recovery (Grade 1), 49.5% Headrice (Grade 1) (Grade 2), Mas malambot at masarap ang lasa kaysa IR64, PSBRc 72H and PSBRc 18. May katamtamang tibay sa Rice Blast and Sheath Blight (SHB) and Arize.

--00--


Filing Date : August 9, 2006


Crop : Corn

Proposed Denomination : CZ076-nm


a) Hybrid Type

CZ076-nm is an inbred line b) Glume base

1 c) Anthocyanin Coloration of glumes excluding base

3 d) Silk color

5 e) Leaf curvature

3 f) Leaf angle

3

Applications


19

g) Stem: degree of zigzag

1 h) Anthocyanin coloration on leaf sheath covering the upper ear

1 i) Presence of Second ear

5 j) Anther color

3 k) Spikelets density

3

Filipino Translation Mga Katangian ng mga Barayti


a) Anthocyanin sa Glume base

wala (1) b) Anthocyanin ng Glume na hindi kasali ang puno

mahina (3) c) Buhok ng puso

medyo mahina (5) d) Kurba ng dahon

bahagyang kurbado (3) e) “Angle” ng Dahon

katamtamang layo (3) f) Zigzag ng puno

wala (1) g) Anthocyanin ng dahong bumabalot sa unang bunga

wala (1) h) Pagkakaroon ng pangalawang bunga

meron (5) i) Anthocyanin ng Anther

mahina (3) j) Density ng spikelets

katamtaman (3) k) Bulaklak

nagkakaroon ng 9-11 sanga

Applications


20




Crop : Corn

Proposed Denomination : CZ172-nm


a) Hybrid Type

CZ172-nm is an inbred line b) Glume base

1 c) Anthocyanin Coloration of glumes excluding base

1 d) Silk color

5 e) Leaf curvature

1 f) Leaf angle

3 g) Stem: degree of zigzag

1 h) Anthocyanin coloration on leaf sheath covering the upper ear

1 i) Presence of Second ear

5 Filipino Translation Mga Katangian ng mga Barayti

Ang CZ172-nm ay isang inbred line.

a) Anthocyanin sa Glume base

wala (1) b) Anthocyanin ng Glume na hindi kasali ang puno

wala (1) c) Buhok ng puso

medyo mahina (5) d) Kurba ng dahon

patayo (1) e) “Angle” ng Dahon

katamtamang layo (3)

Applications


21

f) Zigzag ng puno

bahagya (3) g) Anthocyanin ng dahong bumabalot sa unang bunga

wala (1) h) Pagkakaroon ng pangalawang bunga

meron (5)

--00--




Crop : Corn

Proposed Denomination : BIOSEED 9899


a) Hybrid Type

BIOSEED 9899 is three way cross yellow corn hybrid b) 1st leaf anthocyanin of sheath

7 c) 1st leaf: Shape of tip

7 d) Anthocyanin coloration at glume base

3 e) Anthocyanin Coloration of glumes excluding base

5 f) Anther color

3

Applications


22

g) Silk color

5 h) Leaf curvature

3 i) Leaf angle

3 j) Anthocyanin coloration on leaf sheath covering the upper ear

1 Filipino Translation Mga Katangian ng mga Barayti

Ang BIOSEED 9899 ay isang 3 way hybrid.

a) Anthocyanin ng dahon

matingkad (7) b) Hugis ng unang dahon

ispatula (5) c) Anthocyanin sa Glume base

mahina (3) d) Anthocyanin ng glume na hindi kasali ang puno

matingkad (5) e) Anthocyanin ng Anther

mahina (3) f) Buhok ng puso

mahina (5) g) Kurba ng dahon

bahagyang kurbado (3) h) Anthocyanin ng dahong bumabalot sa unang bunga

wala (1)

Applications


23

NOTICE OF WITHDRAWAL OF APPLICATION FOR CERTIFICATE OF PLANT VARIETY PROTECTION

The public is informed that „Precious’, a pineapple variety applied for

Certificate of Plant Variety Protection by Mt. Kitranglad Agri-

Development Corporation on 30 August 2005 was withdrawn on

30 June 2006.


24

Corn (Zea mays L.)

I. Coverage of These Guidelines

The guidelines for the conduct of distinctness, uniformity and stability tests (DUS) described herein shall apply to all types of varieties of corn, Zea mays L. (inbred, single-cross hybrid, double-cross hybrid, three-way cross hybrid and open-pollinated varieties).

II. Plant Propagation Material Requirement

Upon application, 3,000 viable grains of inbred, hybrid and open pollinated varieties (OPV) together with the application should be submitted to the Plant Variety Protection Office (PVPO) as required in RA 9168. Half of the amount will serve as depository; the rest will be used in the DUS tests. Applicants outside the Philippines must make sure that all custom requirements regarding seed exportation/importation are complied with.

The PVP Board will determine the additional quantity of seeds to be submitted

should the need arise. For open-pollinated varieties, three-way and double-cross hybrids, greater quantities of seeds would be needed since these are genetically more variable than single-cross hybrids and inbred lines.

Seeds must be of high quality and purity, i.e., high germination capacity (at least

90%), free from any pest or diseases, and high level of genetic purity. The level of purity will depend on the type of material being applied for protection. Higher level of genetic purity requirement will be imposed on inbred lines and single-cross hybrids because these materials are usually more uniform than other types.

The seeds should not have undergone any form of treatment unless otherwise allowed by the PVP board in certain circumstances. In such cases, the details of the seed treatment must be clearly described.

III. Conduct of Tests

The minimum duration of the DUS tests should be two similar growing seasons. The tests should be conducted in at least one accredited DUS Testing Center which would be determined by the PVP Board. To facilitate the conduct of the tests, the applicant may submit data from a growing environment similar to that of an accredited DUS testing center where the tests would be carried out to replace one of the required growing seasons.

The comparative growing trials must be carried out under optimum growing

conditions recommended for corn. Each trial should have at least two replications in randomized complete block design. The minimum total number of plants to be tested is 40 for inbred lines and single-cross hybrids, and 80 for other types. For the inbred test, plot size is 1.5m x 5.0m; each plot consisting of two 5-m rows spaced 0.75 m apart. For the other types, each plot is 3.0m x 5.0m consisting of four 5-m rows (0.75 apart).

Test Guidelines


25

There should be 26 hills/row with 2 seeds/hill to be thinned out to one plant/hill one 10-14 days after emergence.

For test of distinctness that involves enzyme electrophoresis, at least 20

coleoptiles should be analyzed. For harmonization with UPOV-member countries, the procedure for isoenzyme analysis for Zea mays L. (Reference TG/2/6) should be used. A copy is available at the PVP office upon request.

IV. Methods and Observations

The different characteristics described in the Table of Characteristics (Part VII) should be used for the testing of distinctness of inbred lines, hybrids and open-pollinated varieties. However, to assess the distinctness of hybrids, a prescreening system based on the parental lines and the hybrid pedigree may be established according to the following:

the parental line should be described according to the Test Guidelines; the originality of the parental lines should be checked in comparison

with the reference collection based on the Table of Characteristics in order to determine the closest inbred line;

the originality of the hybrid pedigree be checked in comparison with those hybrids which are commonly or publicly known in order to determine the closest inbred lines;

the distinctness of the hybrids should be assessed with hybrid varieties of the same formula.

Assessment of distinctness and uniformity should be made on at least 40 plants

or parts of plants excluding outcrossed plants in inbred lines and excluding plants obviously resulting from the selfing of a parent line in single-cross hybrids. All observations on the ear should be made on the upper well-developed ear.

For the assessment of uniformity of inbred lines and single-cross hybrids, a population standard of 3% with an acceptance probability of 95% should be applied. For instance, in a sample of 40 plants, the maximum number of off-types allowed would be three (3). The same population standard and acceptance probability should apply to clear cases of out-crossed plants in inbred lines as well as plants obviously resulting from the selfing of a parent line in single-cross hybrids (e.g. plant height, size of ear or earliness as well as proof through enzyme electrophoresis). For three-way cross hybrids, double-cross hybrids and open-pollinated varieties, the variability within these varieties should not exceed the variability of comparable varieties already known.

In three-way cross and double-cross hybrids, certain characteristics may

segregate such that several states of trait expression occur side by side within a given variety. If experience would show that such traits or characteristics are known to segregate, then such traits or characteristics should be identified with an “S”.

If enzyme electrophoresis is used for testing distinctness, the same population

standard deviation and acceptance probability level as for other characteristics should be applied. However, a sequential analysis approach could be applied to reduce the

Test Guidelines


26

workload. All inbred lines should be considered out-crosses when two or more loci are found heterozygous with one allele of the locus of the inbred line (e.g. AX). All cases where one locus is heterozygous or where two foreign alleles are present should be considered off-types.

V. Grouping of Varieties

To facilitate the assessment of distinctness, the varieties for evaluation should be divided into groups. Characteristics which are suitable for grouping purposes are those which are known from experience not to vary, or to vary only slightly within a variety. The various states of expression should be fairly evenly distributed throughout the collection.

It is recommended that the following characteristics be used for grouping

varieties :

time of anthesis (characteristic 5) anthocyanin coloration of silks (characteristic 14) plant height (characteristic 20) number of rows of grain (characteristic 27) type of grain (characteristic 28)

VI. Characteristics and Symbols

The test for distinctness, uniformity and stability should be based on the characteristics and their state of expressions as listed in the Table of Characteristics.

Following are the legend-description of the symbols used in the Table of

Characteristics :

(*) Refers to characteristics that should be used on all varieties in every growing period over which examinations are made and always included in the variety descriptions, except when the state of expression of a preceding characteristic of certain environmental conditions would render this impossible

(+) See explanations on the Table of Characteristics in Part VIII. See explanations on possible segregation in Part IV.

Test Guidelines


27

VII. Table of Characteristics for Maize

Trait No.

Characteristics Stage1 Description/

Category Reference

(Example)Varieties Note/

Remarks

1 First leaf: anthocyanin coloration of sheath

12 (S) absent

weak

medium

strong

1

3

5

7

2 (+)

First leaf: shape of tip

14 pointed

pointed to round

round

round to spatulate

spatulate

1

2

3

4

5

3 (+)

Tassel: anthocyanin coloration at base of glume (in middle third of main axis)

35-50 (S)

absent

weak

medium

strong

1

3

5

7

4 Tassel: anthocyanin coloration of glumes excluding base (as for trait 3)

35-50 (S)

absent

weak

medium

strong

1

3

5

7

5 (*)

Tassel: time of anthesis (on middle third of main axis, 50% of plants), DAP

40-55 very early (<40)

early (40-45)

medium (45-50)

late (50-55)

very late (>55)

1

3

5

7

9

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28

Trait No.


Category Reference


Remarks

6 (+)

Tassel: anthocyanin coloration of anthers (as for 3; on fresh anthers)

40-55

absent

weak

medium

strong

1

3

5

7

7 Tassel: density of spikelets (as for 3) (main axis)

50-65 sparse

intermediate

dense

1

3

5

8 (*) (+)

Tassel: angle between main axis and lateral branches (in lower third of tassel), degrees

50-65 very short (<30)

short (30-45)

medium (45-60)

large (60-90)

very large (>90)

1

3

5

7

9

9 (*) (+)

Tassel: curvature of lateral branches (as for 8)

50-70 (S)

straight

slight curved

curved

strongly curved

very strongly curved

1

3

5

7

9

10 Tassel: length of main axis above lowest branch, cm

50-70 very short (<30)

short (30-35)

medium (35-40)

long (40-50)

very long (>50)

1

3

5

7

9

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29

Trait No.


Category Reference


Remarks

11 Tassel: length of lowest branch, cm

50-70 very short

short

medium

long

very long

1

3

5

7

9

12 (*)

Tassel: number of primary branches

50-70 absent (0-3)

few (3-10)

medium (10-15)

many (15-20)

very many (>20)

1

3

5

7

9

13 Ear: time of silk emergence (50% of plants), DAP

45-60 very early (<45)

early (45-50)

medium (50-55)

late (55-60)

very late (>60)

1

3

5

7

9

14 (*)

Ear: Intensity of anthocyanin coloration of silks

45-60 (S)

absent

very weak

weak

medium

strong

1

3

5

7

9

15 (+)

Leaf: angle between blade and stem (on leaf just above upper ear), degrees

61 very small (<30)

small (30-45)

medium (45-60)

large (60-90)

very large (>90)

1

3

5

7

9

Test Guidelines


30

Trait No.

Characteristic Stage1 Description/

Category Reference


Remarks

16 (-)

Leaf: curvature of blade (as for trait no 15)

61 straight

slightly curved

curved

strongly curved

very strongly curved

1

3

5

7

9

17 Stem: degree of zig-zag

65 absent

slight

strong

1

3

5

18 Stem: anthocyanin coloration of brace roots

65-75 (S)

absent

weak

medium

strong

1

3

5

7

19 Leaf: anthocyanin coloration of sheath covering upper ear

71 (S) absent

very weak

weak

medium

strong

1

3

5

7

9

20.1 (*)

Inbred lines only; Plant: height (tassel included), cm

75 very short (<.100)

short (100-120)

medium (120-140)

high (140-160)

very high (>160)

1

3

5

7

9

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31

Trait No.


Category Reference


Remarks

20.2 (*)

Hybrids and open-pollinated varieties only: Plant: height (tassel included), cm

75 very short (<175)

short (175-190)

medium (190-205)

high (205-220)

very high (>220)

1

3

5

7

9

21 Plant: Height of uppermost ear to plant height ratio, (%)

75 very low (<30)

low (30-45)

medium (45-55)

high (55-70)

very high (>70)

1

3

5

7

9

22 Leaf: width of blade (leaf of upper ear,) cm

75 very narrow (< 5)

narrow ( 5-6)

medium ( 6-7 )

wide ( 7-8 )

very wide (> 8 )

1

3

5

7

9

23 Ear: length of peduncle, cm

85 very short ( < 4)

short ( 4- 6)

medium ( 6 –8)

long ( 8 – 10)

very long ( > 10)

1

3

5

7

9

24 Ear: Anthocyanin coloration on the peduncle

92 absent

weak

strong

1

3

5

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32

Trait No.


Category Reference


Remarks

25 Ear: diameter (in middle), cm

92 very small (< 3)

small ( 3-4)

medium ( 4-5 )

large ( 5 –6)

very large (> 6)

1

3

5

7

9

26 Ear: Number of husk leaves/ear

92 very few (<5)

few (5-10)

average (11-15)

many (16-20)

very many (>20)

1

3

5

7

9

27 Ear: Length of husk leaves, cm (1 representative sample/ear)

92 very short (<15)

short (16-20)

medium (21-25)

long (26-30)

very long (>30)

1

3

5

7

9

28 Ear: Width of husk, cm (in middle, based on sample in #27)

92 very short (<5)

short (6-10)

medium (11-15)

long (16-20)

very long (>20)

1

3

5

7

9

29 Ear: shape 92 Conical

conico-cylindrical

cylindrical

bear paw

1

3

5

7

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33

Trait No.


Category Reference


Remarks

30 (*)

Ear: length (without husk), cm

92 very short ( < 5 )

short (5 –10 )

medium ( 10-15 )

long (15 – 20)

very long ( > 20)

1

3

5

7

9

31 Ear: diameter (in middle), cm

92 very small (< 3)

small ( 3-4)

medium ( 4-5 )

large ( 5 –6)

very large (> 6)

1

3

5

7

9

32 Ear: Unfilled tip, cm

92 Almost none (< 1 )

Short (1- 2)

Long (>2)

1

3

5

33 Ear: Grain row arrangement

92 Distinct/Regular

Not distinct/Irregular

1

9

34 Ear: number of rows of grain

92 very few (< 10)

few ( 10-12)

medium ( 14)

many ( 16)

very many ( >16)

1

3

5

7

9

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34

Trait No.


Category Reference


Remarks

35 (*)

Ear: type of grain (in middle third of ear)

92 (S) flint

flint-like

intermediate

dent-like

dent

sweet

pop

opaque

waxy

36 Ear: White cap on top of grain, % coverage on whole ear

92 Absent

Small coverage (<25)

Medium

coverage (25-50)

Large coverage (50-75)

Very large

coverage (>75)

1

3

5

7

9

37 (*)

Ear: color of top of grain (disregarding white cap, if present)

92 (S) white

yellowish white

yellow

yellow orange

orange

red orange

red

dark red

blue black

1

2

3

4

5

6

7

8

9

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35

Trait No.


Category Reference


Remarks

38 Ear: color of dorsal side of grain

92 (S) white

yellowish white

yellow

yellow orange

orange

red orange

red

dark red

blue black

1

2

3

4

5

6

7

8

9

39. Ear: Shape of grain 93

40 Ear: intensity of anthocyanin coloration of glumes of cob

93 (S) absent

very weak

weak

medium

strong

1

3

5

7

9

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36

VIII. Explanations on the Table of Characteristics

The following explanations refer to Trait Numbers marked with the symbol „(+)‟ in the Table of Characteristics (Part VII):

Trait No. 2

First Leaf: shape of tip

Trait No. 8 and Trait No. 15

Leaf and tassle: angle

Trait No. 11 and Trait No. 16

Leaf and tassel: curvature of blade and of lateral branches

1

very small (<30)

2

small (30-45)

3

medium (45-60)

4

large (60-90)

5

very large (>90)

2 pointed to round

3 round

4 round to spatulate

5 spatulate

1 pointed

1 straight

2

slightly curved

4

strongly curved

5 very strongly

curved

3 curved

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37

Trait No. 3

Tassel: anthocyanin coloration at base of glume

Growth Stages of Corn*

IX. Reference:

UPOV TG/2/6. 1994-1999. Guidelines for the conduct of tests for distinctness, uniformity and stability for maize (Zea mays L.). International Union for the Protection of New Varieties of Plants (UPOV). 75 p.

Test Guidelines

Tassel 50-52 DAP

Silks 52-58 DAP

Maturity 90-110 DAP

5-7 DAP Emergence Coleoptile

2 Leaves 11-12 DAP

arly

5-6 Leaves 20-24 DAP Early Whorl

9-10 Leaves 32-35 DAP

Mid-Whorl

12 Leaves L48-49 DAP

Whorl

8 Leaves 25-30 DAP Mid-

Whorl

4 Leaves 17-18 DAP Early Whorl


38

X. Technical Questionnaire

TECHNICAL QUESTIONNAIRE (To be completed in connection with an application for plant variety protection)

1. Species: Zea mays L. Maize

2. Name and address of applicant:

3. Proposed denomination or breeders reference:

4. Information on origin, maintenance and reproduction of the variety

4.1 Method of breeding/type of material:

4.2 Other information

(i) inbred line [ ] (ii) single-cross hybrid [ ] (iii) three-way cross hybrid [ ] (iv) double-cross hybrid [ ] (v) open-pollinated variety [ ] (vi) other (please indicate formula [ ]

5. Characteristics of the variety to be given (the number in brackets refers to the correspondingcharacteristic in the Test Guidelines; please mark the state of expression which best corresponds)

Trait No.

Characteristic Description/Category Reference

(Example) Varieties Note/

Remarks

5.2 (16)

Ear: anthocyanin coloration of silks

65 (S)

absent present

1 [ ] 9 [ ]

5.3a (22.1)

In-bred lines only: Plant length (tassel included)

75 very short

short medium

1 [ ] 3 [ ] 5 [ ]

Reference Number: (not to be filled in by the applicant)

Test Guidelines


39

long very long

7 [ ] 9 [ ]

5.3b (22.2)

Hybrids and open pollinated varieties only: Plant: length (tassel included)

75 very short

short medium

long very long

1 [ ] 3 [ ] 5 [ ] 7 [ ] 9 [ ]

5.4 (30)

Ear: type of grain ( in middle third of ear)

92 (S)

flint flint-like

intermediate dent-like

dent sweet pop

1 [ ] 2 [ ] 3 [ ] 4 [ ] 5 [ ] 6 [ ] 7 [ ]

5.5 (33)

Ear: anthocyanin coloration of glumes of cob

93 (S)

absent present

1 [ ] 9 [ ]

6. Similar varieties and differences from these varieties

Denomination of similar variety

Characteristics in which the similar variety is

different

State of expression of similar variety

State of expression of candidate variety

In the case of identical states of expression of both varieties, please indicate the size of the difference.

7. Additional information which may help to distinguish the variety.

a. Resistance to pests and diseases

b. Special conditions for the examination of the variety

c. Other information

end of document

Test Guidelines


40

Rice (Oryza sativa, L.)

I. Subject of these Guidelines

These Test Guidelines apply to all varieties of Oryza sativa L.

II. Material Required

The competent authorities decide on the quantity and quality of the plant material required for testing the variety and when and where it is to be delivered. Applicants submitting material from a Country other than that in which the testing takes place must ensure that all customs formalities and pytosanitary requirements are complied with.

The material is to be supplied in the form of seed.

The minimum quantity of seeds, to be supplied by the applicant, should be:

General : 500 grams

Hybrid varieties : If requested, an additional 500 grams of seed of

each component (A, B and R parental lines) should be submitted.

Panicles : If requested by the competent authority, at least 50

panicles should also be submitted. The panicles should be well-developed and not affected by any pest or disease. They should contain a sufficient number of viable seeds to establish a satisfactory row of plants for observation.

The seed should meet the minimum requirements for germination rate (at least

85%), analytical purity 1.1%, health and moisture content not greater than 14%. In cases where the seed is to be stored, the germination capacity should be as high as possible and should be stated by the applicant.

The seeds should not have undergone any treatment, which would affect the

expression of the characteristics of the variety, unless the competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.

Test Guidelines


41

III. Method of Examination

Duration of Tests

The minimum duration of tests should be 2 independent growing cycles (1 wet and 1 dry).

Testing Place

The tests should normally be conducted in one place. If any characteristic of the variety, which are relevant for the examination of DUS, cannot be seen in that place, the variety may be tested in an additional place.

Conditions for Conducting the Examination

The tests should be carried out under conditions ensuring satisfactory growth for the expression of the relevant characteristics of the variety and for the conduct of the examination.

Characteristics on plants or parts of plants to be selected in a particular way.

Characteristics containing the following notes in the second column of the

Table of Characteristics should be examined as indicated below:

A Leaf: Unless otherwise indicated, all observation on the leaf should be made on the penultimate leaf.

Timing of the examination

The optimum stage of development for the assessment of each characteristic is

indicated by a number in the second column of the Table of Characteristics. The stages of development denoted by these numbers are described at the end of Chapter 8.

Type of observation – visual or measurement

The recommended method of observing the characteristics is indicated by the

following key in the second column of the Table of Characteristics :

MG - single measurement of a group of plants or parts of plants MS - measurement of a number of individual plants or parts of plants VG - visual assessment by a single observation of a group of plants or parts of

plants VS - visual assessment by observation of individual plants or parts of plants

Test Guidelines


42

Test Design

The design of the tests should be such that plants or parts of plants may be removed for measurement or counting without prejudice to the observations which must be made up to the end of the growing cycle.

Each test cycle should be designed to result in a total of at least 400

plants for each candidate variety, which should be divided into two or more replications.

Single panicle- rows if test on panicle-rows are conducted, at least 20

panicle-rows should be observed.

Number of Plants / Parts of Plants to be Examined

Unless otherwise indicated, all observations made on individual plants or

determined by measurement or counting should be made on at least 10 plants or parts taken from each of the 10 plants.

Additional Tests

In cases where morphological characteristics are insufficient to distinguish

candidate variety, additional tests including phenol test on lemma, DNA finger printing etc. maybe conducted.

IV. Assessment of Distinctness, Uniformity and Stability

Distinctness

Recommendation

It is of particular importance for users of this Test Guidelines to consult the General Introduction prior to making decisions regarding distinctness. However, the following points are provided for elaboration or emphasis in these Test Guidelines.

Consistent Differences

The minimum duration of tests recommended in section 3.1 reflects, in

general, the need to ensure that any differences in a character are sufficiently consistent.

Clear Differences

Determining whether a difference between two varieties is cleardepends on many factors, and should consider, in particular, the type of expression of the characteristic being examined, i.e. whether it is expressed in a qualitative, quantitative, or pseudo-qualitative manner.

Test Guidelines


43

Therefore, it is important that users of these Test Guidelines are familiar with the recommendations contained in the General Introduction prior to making decisions regarding distinctness.

Uniformity

It is of particular importance for users of these Test Guidelines to consult the

General Introduction prior to making decisions regarding uniformity. However, the following points are provided for elaboration or emphasis in these Test Guidelines.

Uniformity assessment by off-types

For the assessment of uniformity of characteristics on the plot as a whole

(visual assessment by a single observation of a group of plants or parts of plants), a population standard of 0.1% with an acceptance probability of at least 95% should be applied. In the case of a sample size of 400 plants the maximum number of off-types allowed would be

Hybrid varieties

For the assessment of uniformity of single hybrid, a population

standard of 1% with an acceptance probability of at least 95% should be applied. In the case of a sample size of 400 plants the maximum number of off-types allowed would be 11.

Single panicle-rows

For the assessment of uniformity of characteristics on single panicle-

rows, plants or parts of plants (visual assessment by observations of a number of

individual panicle-rows, plants or parts of plants), a population standards of 1% with an acceptance probability of at least 95% should be applied. In the case of a sample size of 20 panicles the maximum number of aberrant panicle-rows, plants or parts of plants should not exceed 1.

Stability

In practice, it is not usual to perform tests of stability that produce results as certain as those of the testing of distinctness and uniformity. However, experience has demonstrated that, for many types of variety, when a variety has been shown to be uniform, it can also be considered to be stable.

Where appropriate, or in cases of doubt, stability may be tested, either by

growing a further generation, or by testing 500 grams seed or plant stock to ensure it exhibits the same characteristics as those shown by the previous material supplied.

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44

V. Grouping of Varieties and Organization of the Growing Trial

The selection of varieties of common knowledge to be grown in the trial with the candidate varieties and the way in which these varieties are divided into groups to facilitate the assessment of distinctness is aided by the use of grouping characteristics.

Grouping characteristics are those in which the documented states of

expression, even when produced at different locations, can be used, either individually or in combination with other such characteristics: (a) to select varieties of common knowledge that can be included from the growing trial used for examination of distinctness; and (b) to organize the growing trial so that similar varieties are grouped together.

The following have been agreed as useful grouping characteristics:

Basal leaf: sheath color (characteristic no. 1) Days of heading (50% of plants with heads) (characteristic no. 18) Culm length (excluding panicle; excluding floating rice) (characteristic no. 22) Decorticated grain: length (characteristic no. 49) Decorticated grain: color (characteristic no. 52) Endosperm type: (characteristic no. 52)

Guidance for the use of grouping characteristics, in the process of examining

distinctness, is provided through the General Introduction

VI. Introduction to the Table of Characteristics Categories of Characteristics

Standard Test Guidelines Characteristics

Standard Test Guidelines characteristics are those which are approved by

UPOV for examination of DUS and from which members of the Union can select suitable characteristics for their particular circumstances.

Asterisked Characteristics

Asterisked characteristics (denoted by *) are those included in the Test

Guidelines which are important for the International Harmonization of variety descriptions and should always be examined for DUS and included in the variety descriptions by all members on the Union, except when the state of expression of a preceding characteristics or regional environmental conditions render this inappropriate.

Test Guidelines


45

States of Expression and Corresponding Notes

States of expression are given for each characteristic to define the characteristic and to harmonize descriptions. Each state of expression is allocated a corresponding numerical note for ease of recording of data and for the production and exchange of the description.

Types of Expression An explanation of the types of expression of characteristics (qualitative, quantitative and pseudo-qualitative) is provided in the General Introduction.

Reference Varieties

Where appropriate, example varieties are provided to clarify the state of

expression of each characteristic.

Legend (*) Asterisked characteristic – see Section 6.1.2

(QL) Qualitative characteristic – see Section 6.3 (QN) Quantitative characteristic – see Section 6.3 (PQ) Pseudo-Qualitative characteristic – see section 6.3 (+) See Explanation on the Table of Characteristics on Chapter 8.

Stage of development : see Section 3.3.2 MG-MS-VG-VSb : see Section 3.3.3

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46

VII. Table of Characteristics

Char No.

Method of Examination

Characteristics Example Varieties

Note

1. 30 VS

Basal leaf: sheath color green light purple purple lines purple

1

2

3

4

2. 40 VG

Leaf: intensity of green color light medium dark

3

5

7

3. 40 VG

Leaf: anthocyanin coloration absent present

1

9

4. 40 VG

Leaf: distribution of anthocyanin coloration tips only margins only blotches only uniform

1

2

3

4

a

a

Test Guidelines


47

Char No.


Characteristics

Example Varieties

Note

5. 40 VG

Leaf sheath: anthocyanin coloration absent present

1

9

6. 40 VG

Leaf sheath: intensity of anthocyanin coloration light purple purple Lines

purple

3

5

7

7. 40 VS

Leaf: pubescence of blade surface glabrous intermediate pubescent

3

5

7

8. (*)

40 VS

Leaf: anthocyanin coloration of auricles absent present

1

9

9. 40 VS

Leaf: anthocyanin coloration of collar absent present

1

9

10. (+)

VS

Leaf: shape of ligule truncate acute cleft

3

5

7

a

a

a

a

a

Test Guidelines


48

Char No.


Characteristics

Example Varieties

Note

11. 40 VS

Leaf: color of ligule colorless green light purple purple stripes purple

1

2

3

4

5

12. 40 MS

Leaf: length of blade (actual measurement) Short <30 cm Medium 31-60 cm Long > 60 cm

3

5

7

13. 40 MS

Leaf: width of blade Narrow <1 cm Medium 1-2 cm Broad > 2 cm

3

5

7

14. (*) (+)

60 VG

Flag leaf: attitude of blade (early observation) (actual measurement) erect semi-erect horizontal descending

1

3

5

7

Test Guidelines

a

a


49

Char No.


Characteristics

Example Varieties

Note

15. (*) (+)

90 VG

Flag leaf: attitude of blade (late observation) erect semi-erect horizontal descending

1

3

5

7

16. 40 VS

Culm: kneeing ability (for floating rice only) absent present

1

9

17. (+)

40 VS

Culm: Angle erect semi-erect open spreading

1

3

5

9

18. (*)

55 VG

Days of heading (50% of plants with heads) very early (< 70) early (71-80) medium (81-90) late (91-100) very late (> 101)

1

3

5

7

9

Test Guidelines


50

Char No.



Note

19. (*)

65 VS

Lemma: anthocyanin coloration of apex absent red light purple purple deep purple

1

3

5

7

9

20. (*)

65. VS

Spikelet: color of stigma white light green yellow light purple purple

1

2

3

4

5

21. (+)

70. VS

Culm: thickness thin (< 5mm) thick (> 5mm)

1

9

22. (*)

70 VS

Culm: length (excluding panicle; excluding floating rice) very short (< 50 cm) short (51-70 cm) medium (71-90 cm) long (91-110 cm) very long (> 110 cm)

1

3

5

7

9

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51

Char No.



Note

23. (*)

70 VS

Culm: anthocyanin coloration of nodes absent present

1

9

24. 70 VS

Culm: intensity of anthocyanin coloration of nodes light purple purple deep purple

3

5

7

25. 70 VS

Culm: anthocyanin coloration of internodes light gold purple lines purple

3

5

7

26. (*) (+)

72-90 MS

Panicle: length of main axis very short (< 11cm) short (11-20cm) medium (21-30cm) long (31-40cm) very long (> 40cm)

1

3

5

7

9

27. (*) (+)

90 MS

Panicle: curvature of main axis erect semi-erect drooping deflexed

1

3

5

7

Test Guidelines


52

Char No.


Characteristics

Example Varieties

Note

28. (+)

70

Panicle: number per plant few (<8) medium (8-16) many (>16)

3

5

7

29.

60

Panicle: awns absent present

1

9

30. 60 VS

Panicle: color of awns (early observation) light green red purple

3

5

7

31. (*)

70-80 Panicle: distribution of awns tip –awned short & partly awned short & fully awned long & partly awned long & fully awned

1

3

5

7

9

Test Guidelines


53

Char No.



Note

32. 90 VS

Panicle: color of Awns (late observation) straw yellowish gold brown reddish brown light red red light purple purple black

1

2

3

4

5

6

7

8

9

33. (*)

60-80 VS

Spikelet: density of pubescence of lemma glabrous hairs on lemma keel hairs on upper portion short hairs long hairs ( velvety)

1

3

5

7

9

34. (+)

80-90 Spikelet: apicular color white straw brown red purple black

1

2

3

4

5

6

Test Guidelines


54

Char No.


Characteristics

Example Varieties

Note

35. (+)

90 Panicle: presence secondary branching absent present

1

9

36. (+)

90 Panicle: type of secondary branching weak strong clustering

3

5

7

37. (*) (+)

90 Panicle: type of branches compact intermediate open

3

5

7

38. (+)

90 VG

Panicle: exsertion well exserted moderately-well exserted exserted partly exserted enclosed

1

3

5

7

9

Test Guidelines


55

Char No.



Note

39. 90 VG

Days of maturity very early (< 100) early (101-110) medium (111-120) late (121-130) very late (>130)

1

3

5

7

9

40. (+)

92 VG

Leaf: senescene early intermediate late

3

5

7

41. (+)

92 MS

Sterile lemma: Length short (<1.5mm) medium (1.6-2.5mm) long (>2.5 mm but less than the lemma length extra long (> 2.5 mm but greater than the lemma length) asymmetrical

1

3

5

7

9

42.

92 MS

Sterile lemma: color straw gold red purple

1

3

5

7

Test Guidelines


56

Char No.



Note

43. 92 MS

Grain: weight of 1000 fully developed grains by 14 % MC very low low medium high very high

1

3

5

7

9

44. 92. MS

Grain: length very short (<5.52mm) short (5.52-6.84mm) medium (6.85-8.17mm) long (8.18-9.5mm) very long (>9.5mm)

1

3

5

7

9

45. 92. MS

Grain: width narrow (1.20-1.72mm) medium (1.74-2.36mm) broad (2.37-3.0mm) very broad (>3.0mm)

1

3

5

7

Test Guidelines


57

Char No.


Characteristics

Example Varieties

Note

46. 92 VS

Lemma and Palea: color White Straw Gold and gold furrows Brown spots Brown furrow Brown (Tawny) Reddish and light purple Purple spots Purple furrow Purple Black

0

1

2

3

4

5

6

7

8

9

10

47. (*)

92 Decorticated grain: length short (<5.4mm) medium (5.5-6.5mm) long (6.6-7.4mm) very long (> 7.5mm)

1

3

5

7

48. 92 Decorticated grain: width narrow (< 1.1mm) medium (1.1-2.49mm) broad (>2.49mm)

3

5

7

Test Guidelines


58

Char No.



Note

49. (*) (+)

92 Decorticated grain: shape (in lateral View) round (<1.5mm) semi-round (1.5-1.99mm) half spindle-shaped (2.-2.49mm) spindle-shaped (2.5-2.99mm) long spindle-shaped (> 2.99 mm)

1

3

5

7

9

50. (*)

92 Decorticated grain: color white light yellow light brown variegated brown dark brown light red red variegated purple purple dark purple/black

1

2

3

4

5

6

7

8

9

10

51. (+)

92 Endosperm: type glutinous non-glutinous

1

3

Test Guidelines


59

Char No.



Note

52. (+)

92 Endosperm: amylose content (%) waxy (0-2%) very Low (2.1-10%) low (10.1-20%) intermediate (20.1-25%) high (>25%)

1

3

5

7

9

53. (+)

90 Varieties with Endosperm of Polished grain Non-Waxy only: Expression of white Core less than 5% 5-10% 11-20% 21-40% more than 40%

1

3

5

7

9

54. (+)

90 Varieties with Endosperm of Polished Grain Non-Waxy only: expression of white Belly less than 5% 5-10% 11-20% 21-40% more than 40%

1

3

5

7

9

Test Guidelines


60

Char No.


Characteristics

Example Varieties

Note

55. (+)

92 Alkali digestion low intermediate high intermediate high

1

3

5

7

56. (*)

92 Decorticated grain: aroma absent present

1

9


Ad 1 Coleoptile : color Ad 14 Leaf shape of ligule

Ad 18y and late observation

1 erect

3 semi-erect

5 horizontal

7 reflexed

1 Truncate

2 Acute

3 Cleft

Test Guidelines


61

Ad 24, 25, 26 Lemma : anthocyanin coloration and 41 : Spikelet : color and tip

Ad 28 Stem : thickness At the lowest internode Ad 33 and 34 Panicle : length of main axis (33) and curvature of main axis (34)

L

e

r

m

a

1 erect

3 semi-erect

5 drooping

7 deflexed

Test Guidelines


62

Ad 35 Panicle : number per plant [Explanation to be provided by Korea]

Ad 43 and 44 Panicle : secondary branching

Ad 45 Panicle : attitude of branches

0 absent

1 weak

2 strong

3 clustering

1 erect

3 erect to

semi-erect

5

semi-erect

7 semi-erect to

spreading

9 spreading

Test Guidelines


63

Ad 46 Panicle : exsertion

Ad 48 Leaf : senescence The leaves below the flag leaf are observed at the time of harvest for their retention of greenness. State (3), leaves are dead when the grains have become fully ripened; state (5), intermediate (there must be 1 leaf which retains its color); state (7), 2 or more leaves retain their color at maturity. Ad 49 Sterile lemma : length

The measurement is made on each of the two sterile lemmas.

Ad 54 and 55 Grain : phenol reaction of lemma (54)

Varieties with phenol reaction of lemma present only : Grain : coloration with phenol (55)

Method of Testing : Place hulls from 10 grains into a petri dish of 5 cm diameter, and add 5 ml of 1.5% phenol solution; cover the petri dish, and keep at room temperature (not very cold) for one day.

Ad 58 Decorticated grain : shape (in lateral view) (see comment from Japan) length/width round 1 < 1.50 semi-round 2 1.50-1.99 half spindle-shaped 3 2.00-2.49 spindle-shaped 4 2.50-2.99 long spindle-shaped 5 >3.00

7

partly exserted

5 exserted

1

well exserted

Test Guidelines


64

Ad 60 Endosperm : presence of amylose How to differentiate: By observation, glutinous rice has waxy grains, and non-glutinous rice are non-waxy to transparent with various grades according to the amylose content of the endosperm. When it is necessary to differentiate glutinous rice and rice with very low amylose content, chemical analysis is needed. Note : In general, the amylose content of glutinous rice of pure line variety is 0% However, many commercial varieties, especially local, traditional varieties may contain 1-4% of amylose. This is because the waxy gene is recessive, and when outcrossed by ordinary rice pollen, the endosperm becomes non-glutinous. Also, some methods of testing may result in a low % of amylose. Research on chemical structure of waxy rice is still in progress in Japan. Recently various genes (named “dull” genes) for producing semi-waxy rice have been identified At present, amylose content of those semi-waxy rice is not less than 5% though it is not sure if further lower amylose lines will be bred in the future.

The three categories can be simply defined by reaction to KI-I solution; glutinous type endosperm is stained to reddish purple, non-glutinous type to dark blue purple, and intermediate type to reddish – blue purple. Intermediate rice is non-glutinous but with very low amylose, the type which is recently commanding reputation among consumers in East Asian countries. KI-I solution is prepared by mixing 0.1 % I2 solution and 0.2 % KI solution.

Ad 61 Endosperm : content of amylose

Method ISO 6647 should be used.

Modified from IRRI system by rounding due to variability of data especially in cool climates, and adding a rank of very high amylose content considering rice in some area of southern India.

Further discussion may be needed for alternative definition, not using percentage of amylose, but just describing as very low, low, intermediate, high and very high. Ad 62 Varieties with endosperm of amylose absent only. Polished grain : expression of white core Korea to provide illustration Ad 63 Varieties with endosperm of amylose absent only. Polished grain : expression of white belly Korea to provide illustration

Test Guidelines


65

Ad 64 Alkali digestion

Alkali digestion is much easier to test and simpler to observe, and is parallel to results of testing gelatinization temperature. Notes 1.0-2.5 corresponding to gelatilization

temperature of 74.5-80C notes 3.5-5.4 to 70-74 C and notes 5.5-7 to 55-59 C (Juliano and Villareal: Grain Quality Evaluation of World Rices, IRRI, 1993) Ad 65 Decorticated grain : aroma The main component of the aroma in rice is the 2-acetyl-1pyrroline (AcPy). To vaporize this chemical, 10 ml. of a 1.7% solution of KOH should be added to 2 gram of decorticated grains. The aroma, which is similar to that in pop-corn, is released within ten minutes. The level of expression is determined by reference to the example varieties.

Decimal Code for the Growth Stages of Cereals

2-digit Code

General Description

Freekes’ Scale

Additional Remarks on Wheat, Barley, Rye, Oats and Rice

Germination

00 Dry seed

01 Start of imbibition

02 -

03 Imbibition complete

04 -

05 Radicle emerged from caryosis

06 -

07 Coleoptile emerged from caryopsis

08 -

09 Leaf just at coleoptile tip

Seedling growth

10 First leaf through coleoptile

}

} 1 Second leaf visible (Less than 1 cm)

}

11 First leaf unfolded

}

12 2 leaves unfolded }



15 5 leaves unfolded } 50% of laminae unfolded




19 9 or more leaves unfolded

}

Test Guidelines


66

2-digit Code

General Description

Freekes’ Scale


Germination

20 Main shoot only

21 Main shoot and 1 tiller

2 }

22 Main shoot and 2 tillers

} }


} }


} }


} }


}3 }


} }


} }

29 Main shoot and 9 or more tillers

} }

Stem elongation

30 Pseudo stem erection (2)

4- 5 In rice: vegetative lag phase

31 1st node detectable

6} }

} } Jointing stage

32 2nd node detectable

7} }

33 3rd detectable }

} Above crown nodes

34 4th node detectable

}


}


}

37 Flag leaf just visible

8

38 -

39 Flag leaf ligule/collar just visible

9 Pre-boot stage In rice: opposite auricle stage

Booting

40 - Little enlargement of the inflorescence, Early-boot stage

41 Flag leaf sheath extending

42 -

43 Boots just visibly swollen

} Mid-boot stage

44 - } 10

Test Guidelines


67

2-digit Code

General Description

Freekes’ Scale


45 Boots swollen } Late-boot stage

46 -

47 Flag leaf sheath opening

}

48 - }

49 First awns visible } 10.1 In awned forms only

Inflorescence emergence

}

50 } Fist spikelet of } N } N = non-synchronous crops

} inflorescence just

} }

51 } visible } S } S = synchronous crops

52 } } N

} ¼ of inflorescence emerged

} 10.2

53 } } S

} N

54 } ½ of inflorescence emerged

} 10.3

55 } } S

56 } } N

}3/4 of inflorescence emerged

} 10.4

57 } } S

58 } } N

} Emergence of Inflorescence

} 10.5

59 } completed } S

Anthesis

60 } } N

} beginning of anthesis

} 10.51

61 } } S Not easily detectable in barley

In rice: Usually immediately

62 - Following heading

63 -

64 } } N

}Anthesis half - way

} 10.52

65 } } S

66 -

67 -

68 } } N

}Anthesis complete

} 10.53

69 } } S

Milk

Test Guidelines


68

2-digit Code

General Description

Freekes’ Scale


development

70 -

71 Caryopsis watery ripe

10.54

72 -

73 Early milk }

74 - }

75 Medium milk } 11.1 } Increase in solids of liquid endosperm

} } notable when crushing the caryopsis

76 - } } between fingers

}

77 Late milk ]

78 -

79 -

Dough development

80 -

81 -

83 Early dough }

84 - } Fingernail impression not held

85 Soft dough } 11.2

86 - }

87 Hard dough }

88 - Fingernail impression held, inflorescence

Losing chlorophyll

89 -

Ripening

90 - In rice: Terminal spikelets ripened.

91 Caryopsis hard (difficult to divide by thumbnail (3)

11.3

In rice: 50 % of spikelets ripened

92 Caryopsis hard (can no longer be dented by thumbnail) (4)

11.4

In rice: Over 90% of spikelets ripened (5)

93 Caryopsis loosening in daytime

Risk of grain loss by shedding

94 Over-ripe, straw dead and collapsing

95 Seed dormat

96 Viable seed giving 50% germination

97 Seed not dormant

98 Secondary dormarcy

Test Guidelines


69

2-digit Code

General Description

Freekes’ Scale


induced

99 Secondary dormancy lost

Transplanting and recovery (rice only)

T1 Uprooting of seedlings

T2 -

T3 Rooting

T4 -

T5 -

T6 -

T7 Recovery of shoots

T8 -

T9 Resumption of vegetative growth

Reproduced from EUCARPIA Bulletin No. 7, 1974, pages 49-52, with the kind permission of the authors For further information, see J.C. Zadoks, T.T. Chang and C.F. Konzak, EUCARPIA bulletin No 7, 1974, pages 42-52. Notes on the Table

(1) Stage of seedling inoculation with rust in the greenhouse (2) Only applicable to cereals with a prostrate or semi-prostrate early growth habit (3) Ripeness for binder (ca. 16% water content). Chlorophyll of inflorescence largely lost.

(4) Ripeness for combine harvester (< 16% water content) (5) Optimum harvest time.

IX. Literature

“T. Matsuo (ed.) (1993-97): “Science of the Rice Plant (volume 1-3)” Nosan Gyoson Bunka Kyokai (Nobunkyo), Tokyo, Japan.

Vol. 1 Morphology (1993)

Vol. 2 Physiology (1995) Vol. 3 Genetics (1997) Indic (1997)

Test Guidelines


70


TECHNICAL QUESTIONNAIRE

To be completed in connection with an application for plant breeders‟ rights

1. Subject of the Technical Questionnaire

1.1 Latin Name

1.2 Common Name

2. Applicant

Name Address Telephone No. Fax No. E-mail address Breeder (if different from applicant)


Test Guidelines


71

4. Information on the breeding scheme and propagation of the variety

4.1 Breeding Scheme 4.2 Method of Propagating the Variety 4.2.1 Type of material

(a) Line Male fertile line [ ] Male sterile line [ ]

(b) Hybrid

(c) other (specify)

In the case of hybrid varieties, the production scheme for the hybrid and for each

component, the information according to the following Chapters 5 to 7 should be provided on a separate sheet.

Single Hybrid (SH) (……female parent……) x (….male parent…..) N.B. In case of use of male sterility system, please indicate the name of the maintainer line of the parental line. 4.3 other information

Test Guidelines


72

5. Characteristics of the variety to be indicated (the number in brackets refers to the corresponding characteristics in Test Guidelines; please mark the note which best corresponding).

Characteristics Example Varieties Note

5.1 Basal leaf: sheath color (2) green light purple purple lines purple

1

2

3

4

5.2 Leaf: anthocyanin coloration of auricles (10) absent present

1

9

5.3 Time of healing (50% of plants with heads) very early early medium late Very late

1

3

5

7

9

5.4 Stem: length (excluding panicle; excluding (29) floating rice) very short short medium long very long

1

3

5

7

9

5.5 Decorticated grain: (56) Very short Short Medium

1

3

5

Test Guidelines


73

Long Very long

7

9

5.6 Decorticated grain: color (59) White Light brown Variegated brown Dark brown Light red Red Variegated purple Purple Dark purple

1

2

3

4

5

6

7

8

9

6. Similar varieties and difference from these varieties

Denominations(s) of variety(ies) similar to

your candidate variety

Characteristic(s) in which your candidate

variety differs from the similar variety(ies)

Describe the expression of the

characteristics(s) for the similar variety(ies)

Describe the expression of the characteristics(s)

for your candidate variety

(Example) Plant: Height e.g. note 3 Note 7

e.g. short Tall

e.g. 90 cm 130 cm

7. Additional information which may help in the examination of the variety

7.1 In addition to the information provided in section 5 and 6, are there any additional

characteristics, which may help to distinguish the variety?

7.1.1 Resistance to pest and diseases

Yes [ ] No [ ]

(if yes, please provide details)

7.1.2 Other

Yes [ ] No [ ]

(if yes, please provide details)

Test Guidelines


74

7.1.3 Are there any special conditions for growing the variety or conducting the

examination? Yes [ ] No [ ]

7.1.4 If yes, please give details:

7.2 Special conditions for the examination of the variety


8. Authorization

(a) Does the variety require prior authorization for please under legislation concerning

the protection of the environmental, human and animal health? Yes [ ] No [ ]

(b) Has such authorization been obtained? Yes [ ] No [ ] If the answer to (b) is yes, please attach a copy of the authorization.

end of document

Test Guidelines


75

Vegetable Legumes Cowpea (Vigna unguiculata)

Yardlong Beans (Vigna unguiculata var. sesquipedalis) Bush Sitao (Vigna unguiculata x Vigna unguilata var.)


This test guideline applies to all varieties of Vigna unquiculata var. sesquipedales (Yardlong beans); Vigna unguiculata x Vigna unguilata var. sesquipidalis (Bush sitao) and Vigna radiata (Cowpea).


The PVPO decides when, where and in what quantity and quality the seed required for testing the variety to be delivered. Applicants submitting material must make sure that all custom formalities are complied with. The minimum quantity of seed to be supplied by the applicant in one or several samples should be 1000 grams. The seed should have 90% germination, 10% moisture and 100 % purity.

The plant material must not have undergone any treatment unless the

competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.


The minimum duration of tests should normally be two similar growing

periods. The tests should normally be conducted at one place. If any important

characteristics of the variety cannot be seen at that place, the variety may be tested at an additional place.

The tests should be carried out under conditions ensuring normal growth. The

size of the plots should be such that plants or parts of plants may be removed for measurement and counting without prejudice to the observations which must be made up to the end of the growing period. As a minimum, each test should include a total of 60 plants which should be divided between two or more replicates. Separate plot for observation and for measuring can only be used if they have been subjected to similar environmental conditions.

Additional tests for special purposes may be established. Varieties under test

must receive the same treatment for fertilizer, insecticide and or fungicide application, watering and cultivation.

Test Guidelines


76


All observations determined by measurement or counting should be made on 20 plants or parts of 20 plants.

For the assessment of uniformity, a population standard of 2% with an

acceptance probability of 95 % should be applied. The maximum number of off-types allowed would be 3. All observations on the growth habit and on the leaf should be made at the time

of full flowering. All observations on the pod should be made at the time of fresh market maturity. All observations on the seed should be made on dry seed harvested from the pods. The seed weight should be measured on four samples of 100 seeds.

When resistance characteristics are used for assessing distinctness, uniformity

and stability, records must be taken under conditions of controlled infection on at least 20 plants.


The collection of varieties to be grown should be divided into groups to facilitate

the assessment of distinctness. Characteristics which are suitable for grouping purposes are those which are known from experience not to vary, or to vary only slightly, within a variety. Their various states of expression should be evenly distributed throughout the collection.

It is recommended that the competent authorities use maturity in grouping

varieties.


To assess distinctness, uniformity and stability, the characteristics and their states is given in the Table of Characteristics should be used.

Notes (1 to 9) for purposes of electronic data processing, are given opposite the

states of expression for each characteristic.

Legend

(*) Characteristics that should be used on all varieties in every growing period over which the examinations are made and always be included in the variety descriptions, except when the state of expression of a preceding characteristic or regional environmental conditions render this impossible.

(+) See explanations on the Table of Characteristics in Chapter VII.

(-) See picture

Test Guidelines


77


Trait No.

Characteristics Description /

Category

Reference (Example)Varities Note /

Remarks Yardlong

Beans Bush Sitao

Cowpea

1. Seed: 100 seed weight (gms)

small <13 3

medium 13.1 – 17.9

5

large >18

7

2. Seed shape of median longitudinal section (+)

circular 1

circular to elliptical

2

elliptical

3

kidney shaped 4

3.

Seed shape of median cross section Seed shape of median cross section

circular 1

circular to elliptic

2

elliptic

3

circular 1

4.

Seed degree of curvature for kidney shaped seeds only Seed degree of curvature for kidney shaped seeds only

weak 3

medium

5

strong 7

weak 3

5.

Seed width in cross section (cm) Seed width in cross section (cm)

narrow 3

medium

5

broad 7

narrow 3

Test Guidelines


78

Trait No.


Category


Remarks Yardlong

Beans Bush Sitao

Cowpea

6. (*)

Seed length median

short 3

medium

5

long 7

7.

Seed number of colors

one 1

two

2

more than two

3

8.

Seed main color (largest area for 2 or more colors)

white 1

brown

2

black

3

maroon

4

9. Seed predominant secondary color

white 1

brown

2

black

3

10. Seed distribution of predominant secondary color

around hilum 1

in streaks

2

on half of grain 3

in patches at tip

4

11.

Seed color of hilar ring (-)

same color of seed

1

not the same 2

Test Guidelines


79

Trait No . Characteristics

Description / Category


Remarks Yardlong

Beans Bush Sitao

Cowpea

12.

Seed veining Weak 3

Medium

5

Strong

7

13.

Seed surface texture

Smooth 1

Wrinkled

2

14.

Seed number per pod

Low <13 3

Medium 14-17 5

High >18

7

15.

Plant: anthocyanin coloration of hypocotyls (10 days after emergence)

Absent 1

Present 9

16.

Plant: branching habit

Conical 1

Cylindrical

2

17.

Plant: maturity (number of days to 1st harvest/maturity)

Early 3

Medium 5

Late

7

18.

Plant: number of days to 50% flowering

Early 3

Medium

5

Late

7

Test Guidelines


80

Trait No.


Category


Remarks Yardlong

Beans Bush Sitao

Cowpea

19.

Plant: first pod bearing node (*) (number starting on unfoliate node)

Low 3

Medium

5

High

7

20. Plant: height (cms) of the first fruiting (*) node, average of 5 plants

Low 3

Medium

5

High

7

21. Leaf: color Light green 1

Green

2

Dark green 3

Greenish purple

4

Others

9

22. Leaf: petiole color Light green 1

Purple

2

Greenish purple

3

Dark purple 4

23. Leaf: basal petiole color

Green 1

Purple

2

Dark purple

3

Test Guidelines


81

Trait No.

Characteristics



Remarks Yardlong

Beans Bush Sitao

Cowpea

24.

Leaf: petiole length (at 50% flowering) (-)

Small (<15 cms) 3

Medium (15.1 to 18 cms)

5

Large (>18.1 cms)

7

25.

Leaf: leaflet shape (+) (-)

Deltoid

1

Ovate

2

Ovate-lanceolate

3

Lanceolate

4

Long-lanceolate

5

26. Leaf: shape of leaf apex (+) (-)

Acute 1

Acuminate 2

Caudate

3

Aristate

4

27.A

Leaf: shape of leaf base (+) (-) (Center leaf)

Acute 1

Cuneate

2

Truncate

3

27.B Leaf: shape of leaf base (Two sides leaves)

Acute

Cuneate

Oblique

Test Guidelines


82

Trait No.


Category


Remarks Yardlong

Beans Bush Sitao

Cowpea

28.

Leaf: shape of leaf margin (+)

Entire 1

Undulate

2

29. Leaf: venation of the leaf blade (+)

Parallel 1

Pinnate

2

Palmate

3

30.

Flower: color of calyx

Green 1

Greenish purple

2

Purple

3

31. Flower: color of wings (at full bloom)

White

1

Light purple 2

Blue

3

Dark purple

4

32. Flower: color of standard

White 1

Light purple 2

Blue 3

Purple 4

33. Flower: color of keel

White 1

Light purple 2

Blue 3

Dark purple 4

Test Guidelines


83

Trait No.


Category


Remarks Yardlong

Beans Bush Sitao Cowpea

34.

Pod: length of peduncle at first harvest

Short (<20 cms)

1

Medium (21 to 27 cms)

5

Long (>27.1 cms)

9

35. (+)

Pod: length in cms (mean of 10 pods at second harvest)

Very short (<29)

1

Short (30 – 39)

3

Medium (49 – 49)

5

Long (50 – 59)

7

Very long (>60)

9

36. (+)

Pod: width in cms (mean of 10 pods at second harvest)

Narrow 3

Medium

5

broad

7

37.

Pod: shape of cross section (+)

Very flat 1

Pear shaped 2

Round elliptic 3

Figure of eight

4

Test Guidelines


84

Trait No.


Category


Remarks Yardlong


38.

Pod: ground color at mature stage (fresh pod)

Light green 1

Dark green 2

Green

3

Light green with purple shade

4

Purple

5

39.

Pod: wall fiber (taken at dry pod maturity (-)

Fleshy type (fiber adheres to

dry seeds

1

Leathery podded 2

Excessive shattering

3

40.

Pod: beak position (+) (-)

Marginal 1

Non-marginal

2

41.

Pod: beak orientation (+) (-)

Upward 1

Straight

2

Downward 3

42.

Pod: beak color Green 1

Red

2

Purple 3

Others

4

Test Guidelines


85

Trait No. Characteristics



Remarks Yardlong


43. Pod: secondary color

Absent 1

Present 9

44.

Pod: hue of secondary color (-)

Cream 1

Violet

2

45. Pod: stringiness (+) (-)

Absent 1

Present

9

46. Pod: shape of distal part (excluding beak) (-)

Acute 1

Acute to truncate 2

Truncate

3

47. Pod: length of beak

Short 3

Medium

5

Long 7

48. Pod: texture of surface (-)

Smooth 3

Medium rough

5

Rough 7

49. Pod: constrictions (immature stage)

Absent 3

Medium

5

Pronounced 7

50. Pod: shelf life

Short 3

Medium

5

Long

7

Test Guidelines


86

Trait No.

Characteristics



Remarks Yardlong


51. Resistance to Diseases

51.1 Nematode Absent 1

Present 9

51.2 Cowpea rust Absent 1

Present

9

51.3

Pole sitao mosaic virus

Absent 1

Present

9

51.4 Fusarium wilt Absent 1

Present

9

52. Resistance to Pests

52.1 Pod borer, Heliothis armegiera

Absent 1

Present 9

52.2 Pod borer, Maruca testulalis

Absent 1

Present

9

52.3 Black aphids Absent 1

Present

9

52.4 Weevils Absent 1

Present

9

52.5 Bean fly Absent 1

Present

9

53. Stress resistance

53.1 Water logging Absent 1

Present 9

53.2 Drought Absent

1

Present 9

Test Guidelines


87

VIII. Explanation on the Table of Characteristics :

9. Leaflet shape:

10. Shape of leaf apeces:

11. Shape of leaf bases:

12. Shape of leaf margin:

1 2 3 4 5 Ovate Deltoid Ovate-Lanceolate Lanceolate Long-Lanceolate

1 2 3 4 Acute Acuminate Caudate Aristate

1 2 3 Acute Cuneate Truncate

1 2 Entire Undulate

Test Guidelines


88

13. Venation of the leaf blade:

22. Pod cross section:

26. Pod beak position:

27. Pod beak orientation:

1 2 3

Parallel Pinnate Palmante

1 2 3 4 Very flat Pear shape Round elliptic Figure of eight

1 2 Marginal Non-marginal

1 2 3 Upward Straight Downward

Test Guidelines


89

38. Seed shape of median longitudinal section

IX. Literature

Anonymous, 1931: “Beans of New York” in “Vegetables of New York, “Vol. I, Part 2 (Hedrick, U.P., a.o.), State of New York Educational Department , pp. 110

DRIJFHOUT, E., 1978: “Genetic interaction between Phaseolus vulgaris and bean common mosaic virus with implication for strain identification and breeding for resistance, “Agricultural Research Report 872, Centre for Agricultural Publishing and Documentation, Wageningen, NL

UPOV Guidelines for the conduct of Tests for Distinctness, uniformity and stability for French Bean, (Phaseolus vulgaris L.)

Calingasan, M.K. (2002). Introduction, evaluation and selection on Pole Sitao in BPI-Los Baños National Crop Research and Development Center 2002 Annual Report pp. 67-76

Calibo, Juanita G. (2002). Acquisition, conservation and utilization of tomato, eggplant, yardlong beans, squash, vegetable soybeans and mungbeans in BPI-Los Baños National Crop Research and Development Center 2002 Annual Report pp. 19-35.

Test Guidelines

1 2 3 4 Circular Circular to elliptical Elliptical Kindney-shaped


90



to be completed in connection with an application for plant breeders‟ rights

1. Species : Yardlong beans, Vigna unquiculata variety sesquipedalis Bush sitao, Vigna unguiculata x Vigna unguiculata var. sesq Cowpea, Vigna unguiculata

2. Applicant (Name and address)

3. Proposed denomination or breeder‟s reference


5. Characteristics of the variety to be indicated (the number in brackets refers to the

corresponding characteristic in the Test Guidelines; please mark the state of expression which best corresponds)

Characteristics Description/Category Example Variety Note

5.3 Number of days to 50% flowering

Early Medium Late

1( ) 5( ) 9( )

5.35 Pod length (cm) Very short < 29 Short 30-39 Medium 40-49 Long 50-59 Very long > 60

1 3 5 7 9

5.38 Pod ground color Green Purple

1 2

5.39 Pod wall fiber Fleshy Leathery Excessive shattering

1 2 3


Test Guidelines


91

Test Guidelines

Characteristics Description/Category Example Variety

Note

5.3 Number of days to 50% flowering

Early Medium Late

1( ) 5( ) 9( )

5.35 Pod length (cm) Very short < 29 Short 30-39 Medium 40-49 Long 50-59 Very long > 60

1 3 5 7 9

5.38 Pod ground color Green Purple

1 2

5.39 Pod wall fiber Fleshy Leathery Excessive shattering

1 2 3

5.45 Pod stringiness Absent Present

1 2

5.1 100 seed weight (g) Light < 13 Medium 13.1-17.9 Heavy > 18

3 5 9

5.8 Seed main color White Brown Black Other

1 2 3 4

5.14 Seed number per pod

Low <13 Medium 14-17 High 18 >

3 5 9



Characteristics in which the similar variety is

different



Note : In case of identical state of expression of both varieties, please indicate the size of the difference


92

7. Additional information which may help to distinguish the variety

7.1 Resistance to pests and diseases :

Absent Present Not tested

Diseases [ ] [ ] [ ]

Nematode [ ] [ ] [ ]

Cowpea rust [ ] [ ] [ ]

Pole sitao mosaic virus [ ] [ ] [ ]

Fusarium wilt [ ] [ ] [ ]

Pests

Pod borer, Heliothis armigera [ ] [ ] [ ]

Pod borer, Maruka testulalis [ ] [ ] [ ]

Black aphids [ ] [ ] [ ]

Bean fly [ ] [ ] [ ]

[ ] [ ] [ ]


( I ) Type of culture

under glass [ ]

open field [ ]

( ii ) Part mainly consumed

pod [ ]

seed [ ]

( iii ) Main use

fresh market or garden [ ]

canning (indicate type) [ ]

( iv ) Other conditions


end of document

Test Guidelines


93

Eggplant (Solanum melongena L.)


These Test Guidelines apply to all varieties of Solanum melongena L.


The competent authorities decide when, where and in what quantity and quality the seed required for testing the variety is to be delivered. Applicants submitting material from a State other than that in which the testing takes place must make sure that all customs formalities are complied with. The minimum quantity of plant material to be supplied by the applicant in one or several samples should be :

15 g.

The quantity of the seed to be delivered should not be below the standards of seeds for certification or marketing in the country concerned, especially in regard to germination capacity and moisture content.


competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.


The minimum duration of tests should be two similar growing periods.

The tests should normally be conducted at one place. If any important characteristics of the variety cannot be seen at that place, the variety may be tested at an additional place.

The tests should carried out under conditions ensuring normal growth. The

size of the plots should be such that plants or parts of plants may be removed for measurement and counting without prejudice to the observations which must be made up to the end of the growing period. As a minimum, each test should include a total of 20 plants. Separate plots for observation and for measuring can only be used if they have been subject to similar environmental conditions.

Additional tests for special purposes may be established.

Test Guidelines


94


All observations determined by measurement or counting should be made on 20 plants or parts of 20 plants.

All observations on the leaf should be made on flowering branches at the early

flowering stage.

All observations on the fruits should be made on normally developed fruits during the 2nd-3rd harvest period.


The collection to be grown should be divided into groups to facilitate the assessment of distinctness. Characteristics which are suitable for grouping purposes are those which are known from experience not to vary or to vary only slightly, within a variety and which in their various states are fairly evenly distributed within the collection.

It is recommended that the competent authorities used the following

characteristics for grouping varieties:

Fruit: length (characteristic 17) Fruit: ratio length/diameter (characteristic 19) Fruit: general shape (characteristic 20) Fruit: color of skin at commercial harvesting (characteristic 25) Fruit: stripes (characteristic 27) Fruit: color of flesh (characteristic 38)

VI. Characteristics and symbols

To assess distinctness, homogeneity and stability, the characteristics in the Table of Characteristics should be used.

Notes (1 to 9), for the purposes of electronic data processing, are given

opposite the states of the different characteristics.

Legend:

(*) Characteristics that should be used every growing period for the examinations of all varieties and should always be included in the description of the variety, except when the states of expression of a proceeding characteristics or regional environmental conditions render this impossible.

(+) See explanations of the Table of Characteristics in Chapter VIII.

Test Guidelines


95


Traits Characteristics Description/Category Reference


Remarks

1. (*)

Seedling: anthocyanin coloration of hypocotyls

absent 1

present 9

2.

Seedling: intensity of anthocyanin coloration of hypocotyls

weak 3

medium 5

strong 7

3. (*)

Plant: attitude/growth habit

erect 3

semi-erect 5

prostrate/spreading 7

4.

Plant: height hort

3

medium

5

tall

7

5. Length of stem from cotyledon to node of first flower)

short

3

medium

5

long

7

6. (*)

Stem: anthocyanin coloration

absent

1

present

9

7.

Stem: intensity of anthocyanin coloration

weak

3

medium

5

strong

7

8.

Stem: hairiness weak

3

medium

5

strong

7

Test Guidelines


96



Remarks

9.

Branch: length of internodes

short

3

medium

5

long

7

10.

Leaf: size small

3

medium

5

large

7

11. (*) (+)

Leaf: margin entire

1

dentate

2

sinuate

3

12.

Leaf: degree of sinuation of margin

weak

3

medium

5

strong

7

13.

Leaf: blistering

absent

1

present

9

strong

7

14.

Leaf: spininess absent or very weak

1

weak

3

medium

5

strong

7

very strong

9

15. (*)

Leaf: color green

1

bluish green

2

violet green

3

Test Guidelines


97



Remarks

16. Flower: purple color

light

3

medium

5

dark

7

17. (*)

Fruit: length

short

3

medium

5

long 7

18. Fruit: diameter

small

3

medium

5

large

7

19. (*)

Fruit: ratio length/diameter

very small

1

small

3

medium

5

large

7

very large

9

20. (*) (+)

Fruit: general shape pear-shaped

1

ovoid

2

globular

3

cylindrical 4

21. (*)

Fruit: size of pistil scar

small

3

medium

5

large

7

22. (*) (+)

Fruit: shape of apex indented

1

rounded

2

pointed

3

Test Guidelines


98



Remarks

23. Fruit: depth of indentation of apex

shallow

3

medium

5

deep

7

24. (*)

Fruit: curvature (only for cylindrical types)

absent or very weak

1

weak

3

medium

5

strong

7

very strong

9

25. (*)

Fruit: color of skin at commercial harvesting

white

1

yellow

2

green

3

mauve

4

purple 5

26. Fruit: intensity of color of skin (except for white; as for 25)

light

3

medium

5

dark

7

27. (*)

Fruit: stripes absent

1

present

9

28. Fruit: density of stripes

sparse

3

medium

5

dense

7

29. Fruit: glossiness at harvest maturity

weak

3

medium

5

strong

7

Test Guidelines


99



Remarks

30. (*)

Fruit: ribs absent

1

present

9

31. Fruit: prominence of ribs

weak

3

medium

5

strong

7

32. Fruit: size of calyx small 3

medium

5

large

7

33. (*)

Fruit: anthocyanin coloration lower portion of calyx

absent

1

present

9

34. Fruit: intensity of anthocyanin coloration lower portion of calyx

weak

3

medium

5

strong

7

35. (*)

Fruit: anthocyanin coloration of calyx

absent

1

present

9

36.

Fruit: intensity of anthocyanin coloration of calyx

very weak

1

weak

3

medium

5

strong

7

very strong 9

37. Fruit: spininess of calyx

absent or very weak

1

weak

3

medium 5

strong

7

very strong 9

Test Guidelines


100



Remarks

38. (*)

Fruit: color of flesh whitish 1

greenish 2

39. Fruit: color of skin at physiological ripeness

yellow

1

ochre

2

brown

3

40. (*)

Time of beginning of flowering

early

3

medium 5

late

7

41. (*)

Time of physiological ripeness

early

3

medium

5

late

7

42. Fruit firmness soft

3

medium

5

firm

7

43. Fruit shelf life (under ambient condition; average no of days from harvest to 50% of the fruits still marketable)

short

3

medium

5

long 7

44. Resistance to Leaf hopper (Rating scale: 1-least damage to 5-most severe damage; data taken from field screening)

susceptible

3

intermediate

5

resistant 7

45. Resistance to Eggplant shoot and fruit borer (ESFB) (Rating of EFSB based on fruit damaged; data taken from field screening)

susceptible

3

intermediate

5

resistant 7

Test Guidelines


101

Traits

Characteristics Description/Category Reference


Remarks

46. Resistance to Bacterial Wilt (based on seedling screening)

susceptible

3

intermediate

5

resistant 7

47. Resistance to Phomopsis

susceptible

3

intermediate

5

resistant 7

Test Guidelines


102


Leaf : margin

Fruit : general shape

Fruit: shape of apex

1 2 3 entire dentate sinuate

1 2 3 4 pear-shaped ovoid globular cylindrical

1 2 3 indented rounded pointed

Test Guidelines


103

IX. Literature

Khan, R., 1979: “Solanum melongena and the problems of its origin and phylogenetic affinities,” J. Indian Bot. Soc., 58; pp. 99-109

Khan, R., 1979: “Solanum melongena and its ancestral forms,” In: Hawkes, J.G., Lester, R.N. and Skelding, A.D. eds.: “The biology and taxonomy of the Solanaceae,” London, Academic Press, pp. 629-636

Martin, F.W. and Rhodes, A.M., 1979: “Subspecific grouping of egg plant cultivars,“ Euphytica 28, pp. 367-383

Pearce, K., and Lester, R.N., 1979: “Chemotaxonomy of the cultivated egg plant – a new look at the taxonomic relationships of Solanum melongena.” In: Hawkes, J.G., Lester, R.N., and Skelding, A.D., eds.: “The biology and taxonomy of the Solanaceae.” London, Academic Press, pp. 616-628

Tindall, H.D., 1983: “Local Garden Egg.” In: “Vegetables in the Tropics.” London, MacMillan Press, pp. 360-365

Test Guidelines


104



to be completed in connection with an application for plant breeder‟s rights

1. Species : Solanum melongena L. EGG PLANT




5. Characteristics of the variety to be given (the number in brackets refers to the corresponding characteristics in the Rest Guidelines; please mark the state of expression which best corresponds)

Characteristics Description/Category

Reference (Example)Varieties

Note/ Remarks

5.1 (17)

Fruit: length

short medium long

3

5

7

5.2 (19)

Fruit: ratio length/ diameter

Very small Small Medium Large Very large

1

3

5

7

9


Test Guidelines


105


Reference (Example)Varieties

Note/ Remarks

5.3 (20)

Fruit: general shape Pear-shaped Ovoid Globular Cylindrical

1

2

3

4

5.4 (21)

Fruit: size of pistil scar

Small Medium Large

3

5

7

5.5 (22)

Fruit: shape of apex Indented Rounded Pointed

1

2

3

5.6 (24)

Fruit: curvature (only for cylindrical types)

Absent or very weak Weak Medium Strong Very strong

1 [ ]

3 [ ]

5 [ ]

7 [ ]

9 [ ]

5.7 (25)

Fruit: color of skin at commercial harvesting

White Yellow Green Mauve Purple

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

5.8 (27)

Fruit: stripes Absent Present

1 [ ]

9 [ ]

5.9 (35)

Fruit: anthocyanin coloration of calyx

Absent Present

1 [ ]

9 [ ]

Test Guidelines


106

Characteristics Description/Category Reference


Remarks

5.10 (38)

Fruit: color of flesh Whitish Greenish

1 [ ]

2 [ ]


Denomination of varieties Differences

7. Additional information which may help to distinguish the variety

7.1 Resistance to pests and diseases



end of document

Test Guidelines


107

Watermelon (Citrullus lanatus (Thunb.) Matsum. et Nakai.)


These Test Guidelines apply to all varieties of Citrullus lanatus, (Thunb.)

Matsum. et Nakai.


The Plant Variety Protection Office (PVPO) decides, when, where and in what quantity and quality the seed required for testing the variety is to be delivered. Applicants outside the Philippines must make sure that all custom formalities are complied with. As a minimum, for each year of test the following quantity of seed is recommended. 400 seeds


competent authorities allow or request such treatment. It has been treated, full details of the treatment must be given.


The minimum duration of tests should be two similar growing periods.

The tests should be normally conducted in one place. If any important characteristics of the variety can not be seen at that place, the variety maybe tested at an additional place.

The tests should be carried out under conditions ensuring normal growth.

The size of the plots should be such that plants or plant parts of plants maybe removed for measurement and counting without prejudice to observations which must be made up to the end of the growing period. As a minimum, each test should include 35 plants. In all cases the total number of plants should be divided between two or more replicates. Separate plots for observation and for measuring can only be used if they have been subject to similar environmental condition.



All plants indicated under Chapter III above should be used for the testing of uniformity. A population standard of 1% and an acceptance probability of 95% should be applied. The maximum number of types allowed would be one.

Unless otherwise indicated, all observations determined by measurement

should b e made on 20 plants or parts of 20 plants.

Test Guidelines


108

All observations on the leaf should be recorded on fully developed leaves during the appearance of the first female flower.

Unless otherwise indicated, all observations on the fruit should be made on

first well developed, mature fruits.

All observations on the seeds should be made on mature and dry seeds after washing and drying in the shade.

When resistance characteristics are used for assessing distinctness.

homogeneity and stability, records must be taken under conditions of controlled infection.


The collection to be grown should be divided into groups to facilitate he assessment of distinctness. Characteristics which are suitable for grouping purposes are those which are known from experience not to vary, or to vary only slightly, within a variety and which in their various states are fairly distributed within the collection.

It is recommended that the competent authorities use the following

characteristics for grouping varieties:

Ploidy : (Characteristic 1) Fruit : shape of longitudinal section (characteristic 29) Fruit : ground color of skin (characteristic 30)


To assess distinctness, homogeneity and stability, the characteristics in the Table of characteristics should be used.

Notes (1-9), for the purposes of electronic data processing, are given opposite

the states of the different characteristics

Legend

(*) Characteristics that should be used every growing period for the examinations of all the varieties and should always be included in the description of the variety, except when the state of expression of a preceding characteristic or regional environmental conditions render this impossible.

(+) See explanations on the Table of Characteristics in Chapter VIII.

Test Guidelines


109


Traits Characteristics Description/Category Reference Varieties

Note/ Remarks

1 (*)

Ploidy

diploid

2

triploid

3

tetraploid 4

(*) 2 Seed: size very small 1

small

3

medium

5

large

7

very large 9

(*) 3 Seed: ground color of testa

white

1

cream

2

green

3

red

4

light brown

red brown

5

brown

6

black

7

4 Seed: secondary color of testa

absent

1

present 9

5 Seed: type of distribution of secondary color of testa

in dots only

1

in patches only

2

in dots and in patches

3

Test Guidelines


110

Traits

Characteristics Description/Category Reference Varieties

Note/ Remarks

6 Seed: area of secondary color in relation to that of ground color

small

3

medium

5

large 7

7 Seed: patches at hilum

absent

1

present 9

8 Seed: patches at margin

absent 1

present 9

9 (*)

Seedling: shape of Cotyledon

narrow elliptic

1

elliptic

2

broad elliptic 3

10 Seedling: size of cotyledon

small

3

medium

5

large 7

11 Seedling: Intensity of green color of cotyledon

light

3

medium

5

dark 7

12 (*)

Seedling: Spots on Cotyledon

absent

1

present

9

13 Seedling: Depression of nerves of Cotyledon

absent

1

present 9

Test Guidelines


111


Note/ Remarks

14 Seedling: Length of hypocotyls

short

3

medium

5

long 7

15 Plant: growth habit bush 1

runner 2

16 Plant: Length of main stem (at the time of harvest of first fruit)

short

3

medium

5

long 7

17 (*)

Plant: Hermaphrodite flowers

absent

1

present 9

18 Plant: Number of nodes up to the first node with female flowers

low

3

medium

5

high

7

19 (*)

Time of female flowering (50% of plants with at least one female flower from sowing)

early

3

medium

5

late 7

20 Time of maturity (50% of plants with at least one ripe fruit from sowing)

early

3

medium

5

late

7

21 (*)

Leaf Blade: Length

short

3

medium

5

long 7

Test Guidelines


112


Note/ Remarks

22 (*)

Leaf Blade: width

narrow

3

medium

5

broad

7

23 Leaf Blade: Ratio length/width

small

3

medium

5

large 7

24 Leaf /blade: color yellow green

1

green

2

gray green 3

25 Leaf/blade: intensity of color

light

3

medium

5

dark 7

26 Leaf: Degree of lobing (beyond first flower)

weak

3

medium 5

strong 7

27 (*) (+)

Leaf: depth of incisions of margin of leaf of central third of plant (as of 17)

shallow

3

medium

5

deep

7

28 Leaf Blade: blistering

weak

3

medium

5

strong 7

29 Leaf Blade: undulation of margin

weak

3

medium

5

strong

7

Test Guidelines


113


Note/ Remarks

30 (*)

Leaf Blade: flecking absent

1

present 9

31 Petiole: length

short

3

medium

5

long

7

32 Flower: size of petal of female flower (third to seventh flower)

small

3

medium

5

large

7

33 Flower: Shape of apex of petal of female flower

acute

3

rounded

5

obtuse 7

34 Flower: anther dehiscence at low temperature

weak

3

medium 5

strong 7

35 Ovary: size small 3

medium

5

large

7

36 Ovary: Pubescence Weak

3

medium

5

strong 7

Test Guidelines


114


Note/ Remarks

37 Fruit: weight (kg)

very low

1

very low to low

2

low

3

low to medium

4

medium

5

medium to high 6

high

7

high to very high

8

high 9

(*) 38 (+)

Fruit: shape of longitudinal section

round

1

broad elliptic

2

elliptic

3

cylindric 4

(*) 39 (+)

Fruit: ground color of skin

white

1

yellow

2

green 3

(*) 40 Fruit: intensity of green color of skin

very light

1

very light to light

2

light

3

light to medium

4

medium

5

medium to dark

6

dark

7

dark to very dark

8

very dark 9

Test Guidelines


115


Note/ Remarks

41 Fruit: length of peduncle

short

3

medium

5

long 7

42 (+)

Fruit: size of insertion of peduncle

small

3

medium

5

large 7

(*) 43 (+)

Fruit: shape of basal part

flat 1

flat to rounded

2

rounded

3

rounded to conical

4

conical 5

44 Fruit: depression of base

shallow

3

medium 5

deep 7

(*) 45 (+)

Fruit: shape of apical part

Flat

1

flat to rounded 2

rounded 3

rounded to conical 4

conical 5

46 Fruit: depression at apex

shallow

3

medium

5

deep 7

Test Guidelines


116


Note/ Remarks

47 Fruit: size of pistil scar

small

3

medium

5

Large

7

(*) 48 Fruit: grooves Absent

1

at basal half

2

at apical half

3

on whole fruit

4

(*) 49 (+)


1

present 9

(*) 50 (+

Fruit: intensity of green color of stripes

very light

1

light

3

medium

5

dark

7

very dark 9

51 Fruit: width of stripes

very narrow

1

narrow

3

medium

5

broad

7

very broad 9

52 Fruit: marbling absent

1

present

9

Test Guidelines


117


Note/ Remarks

53 Fruit: intensity of marbling

very weak

1

weak

3

medium

5

strong

7

very strong 9

(*) 54 (+)

Fruit: thickness of outer layer of pericarp

thin

3

medium

5

thick 7

(*) 55

Fruit: main color of flesh

white

1

yellow

2

orange

3

red

4

purple

5

others (pinkish red) 7

56 Fruit: intensity of main color of flesh

light

3

medium

5

dark 7

57 Fruit: firmness of flesh

soft

3

medium

5

firm 7

Test Guidelines


118


Note/ Remarks

58 Fruit: number of seeds

absent or very few 1

few

3

medium

5

many

7

very many 9

59(+) Resistance to Fusarium oxysporum f.sp. niveum (E.F. Smith) Snyder et Hansen

absent

1

present 9

60 Resistance to Colletotrichum lagenarium (passerini) Ellis et Halsted

absent

1

present 9

61 Resistance to Downy mildew

absent

1

present

9

62 Resistance to Powdery mildew

absent

1

present 9

63 Resistance to Watermelon mosaic

absent

1

present 9

64 Resistance to aphids

absent

1

present

9

65 Resistance to fruit worm

absent

1

present

9

Test Guidelines


119


Note/ Remarks

66 Resistance to thrips absent

1

present

9

67 Resistance to whitefly

absent

1

present

9

VIII. Explanation on the Table of Characteristics

Trait No. 2 Seedlings : shape of cotyledon

Trait No. 18 Leaf blade : depth of incisions of margin of leaf of central third of plant The incisions should be observed at the largest leaf between the fifteenth and twentieth node of the main stem.

shallow medium deep

narrow elliptic elliptic broad elliptic

Test Guidelines


120

Trait No. 29 Fruit : Shape of longitudinal section

Traits No. 30, 40, 41 Fruit : ground color of skin and color of stripes The ground color is defined as the lighter color and the color of the stripes as the darker color.

Traits No. 33, 34, 36, 45 Fruit

basal part (34)

thickness of out layer of pericarp

apical part

insertion of peduncle

1 round

2 broad elliptic

3 elliptic

4 cylindric

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121

Trait No. 59 Resistance to Fusarium oxysporum f. sp. niveum (E. F. Smith) Snyder et Hansen

Method

Maintenance of races

Type of medium: Special conditions: Preparation of inoculum:

P.S.A. (Potatio, Sugar and Agar) medium Stored below 5oC Shaking culture in P. S. (Potato and Sugar) liquid medium for 7 to 10 days at 28oC. Filtration by using double gauzes. Adjusting concentration of spore to 1.5 x 107/ml with sterilized water.

Execution of test

Sowing the seeds: Growth stage of plants: Method of inoculation: Number of plants tested:

In sterilized soil Expanding of first true leaf Soaking of roots and of hypocotyls axis for one minute in inoculum solution. After inoculation, transportation of plantlets in sterilized (by steam) soil or perlite. 10 to 20 plants

Environmental condition after inoculation

Temperature: Light: Growing method:

Day: 25oC; night: 16oC Natural (longer than 12 hours) In the glasshouse or climatic room. Application of liquid fertilizer every week.

Duration of test

Inoculation – last observation:

20 days. Disease symptoms appear from 5 to 10 days after inoculation. Observation should be made on several occasions.

Remarks

Keeping of pathogenecity: Standard varieties:

Renewal of medium at least once a year.

Race 0 Race 1 Race 2

Black Diamond, Kaho S S S

Charleston Gray R S S

Calhoun Gray R R R

P.I. 296341-FR R R R

S: susceptible R: resistant

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122

Trait No. 60 Resistance to Colletotrichum lagenarium (passerini) Ellis et Halsted

Maintenance of races

Type of medium: Special conditions: Preparation of inoculum:

P.S.A. (Potatio, Sugar and Agar) medium Stored below 5oC Shaking culture in P. S. (Potato and Sugar) liquid medium for 7 to 10 days at 28oC. Filtration by using double gauzes. Adjusting concentration of spore to 1.5 x 107/ml with sterilized water.

Execution of test

Sowing the seeds: Growth stage of plants: Method of inoculation: Number of plants tested:

In sterilized soil Expanding of 2nd to 3rd true leaf Inoculated plants should be placed in a dark and humid chamber at 25oC with 100% relative humidity for 48 hours before being moved to glasshouse. 10 to 20 plants

Environmental condition after inoculation

Temperature: Light: Growing method:

Day: 25oC; night: 16oC Natural (longer than 12 hours) In the glasshouse

Duration of test

Inoculation – last observation: 25 days

Remarks

Race: Keeping of pathogenecity: Standard varieties:

Three races are identified Renewal of medium at least once a year.

Race 0 Race 1 Race 2

Kaho S S S

Charleston Gray Congo

R S R

African citron W-695 S R S

S: susceptible R: resistant

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123

IX. Literature

CRALL, J.M., 1959: “Effect of Seed source on Watermelon Maturity,” Proc.Amer.Soc.Hort.Sci. 74, pp 555-557

CRALL, J.M., MONTELARO, J., 1972: “Fusarium Wilt Resistance in Jubilee Watermelon,” Proc.Fra. State Hoest. Soc. 85, pp 102-105

Cucurbit Genetics Cooperative, Cucurbit Gene List Committee, 1987: “Gene List for Watermelon,” Cucurbit Gent. Coop. Rpt. 10, pp 106-110

ELMSTROM, G.W., HOPKINS, D.L., 1981: “Resistance of Watermelon Cultivars to Fusarium Wilt” Plant Disease 65(10), pp 825-827

KANDA, T., 1951: “Triploid Watermelons,” Proc.Am.Soc.Hortic.Sci.58, pp 217-230

KENSLER, T.R., BARHAM, W.S., 1958: “The Inheritance of Seed Size in Watermelon,” Proc.Amer.Soc. Hort.Sci. 71, pp 480-484

MARTYN, R.D., MCLAUGHLYN, R.J., 1983: “Susceptibility of Summer Squash to the Watermelon Wilt Pathogen (Fusarium osyxporum f. sp. niveum),” Plant Disease 67(3), pp 263-266

MARTYN, R.D., NETZER, D., 1991: “Resistance to Race 0, 1 and 2 of Fusarium Wilt of Watermelon in Citrullus sp.,” PI-296341-FR

MIZUNO, S., PRATT, H.K., 1973: “Relations of Respiration and Ethylene Production to Matury in the Watermelon,” J.Amer.Soc.Hort.Sci. 98(6), pp 614-617

MOHR, H.C., 1963: “Utilization of the Genetic Character for Short-internode in Improvement of the Watermelon”, J.Amer.Soc.Hort.Sci. 82, pp 454-459

POOL, C.F., PORTER, D.R., 1933: “pollen Gemination and Development in Watermelon,” Proc.Amer.Soc.Hort.Sci. 30, pp 526-530

POOL, C.F., GRIMBALL, P.C., PORTER, D.R., 1941: “Inheritance of Seed Characters in Watermelon,” Jour.Agr.Res. 66, pp 433-456

SHIMOTSUMA, M., JONES, C.M., 1972: “Effect of Ethephon and Daylight on Sex Expression of Muskmelon and Watermelon,” Hort.Sci. 7, pp 73-75

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124


TECHNICAL QUESTIONAIRE to be completed in connection with an application for plant breeders‟ rights

1. Species : Citrullus lanatus (Thumb.) Matsum. Et Nakai WATERMELON




4.1 Method of maintenance and reproduction

Hybrid [ ] Open pollinated variety [ ]

4.2 Other Information

5. Characteristics of the variety to be given (the number in brackets refers to the corresponding characteristic in the Test Guidelines; please mark the state of expression which best corresponds.

Characteristics Description/Category Example Varieties Note

5.1 (1)

Ploidy diploid

triploid

tetraploid

2 [ ]

3 [ ]

4 [ ]


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125

Characteristics Description/Category Example Varieties

Note

5.2 (37)

Fruit: weight very low

very low to low

low

low to medium

medium

medium to high

high

high to very high

very high

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

6 [ ]

7 [ ]

8 [ ]

9 [ ]

5.3 (38)

Fruit: shape of longitudinal section

round

broad elliptic

elliptic

cylindric

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5.4 (39)

Fruit: ground color of skin

white

yellow

green

1 [ ]

2 [ ]

3 [ ]

55. (49)


present

1 [ ]

9 [ ]

5.6 (55)

Fruit: main color of flesh

white

yellow

orange

red

purple

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

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126



Characteristic in which the similar

variety is different1


State of expression of

candidate variety

1 In the case of identical states of expression of both varieties, please indicate the size of the difference.


7.1 Resistance to pests and diseases

Absent Present Not Tested

(i) Fusarium oxysporum f. niveum (E.F. Smith) Snyder et Hansen Race 0 Race 1 Race 3

[ ] [ ] [ ]

[ ] [ ] [ ]

[ ] [ ] [ ]

(ii) Colletotrichum lagenarium (passerini) Ellis et Halsted Race 0 Race 1 Race 3

[ ] [ ] [ ]

[ ] [ ] [ ]

[ ] [ ] [ ]

(iii) Other resistance (specify) Downy mildew Powdery mildew Aphids Thrips White flies Fruit Worm


(i) Use - in the greenhouse - in the open

- in the open and in greenhouse (ii) Other conditions

[ ] [ ] [ ]


end of document

Test Guidelines


127

Onion and Shallot (Allium cepa L, Allium cepa var. ascalonicum L.)


These Test Guidelines apply to all seed and vegetatively propagated varieties of

onion (Allium cepa L.) and multiplier onion or shallot (A. cepa var. ascalonicum)


The Plant Variety Protection Office (PVPO) decides where and in what quantity and quality the plant material required for testing the variety is to be delivered. Applicants submitting material outside the Philippines must make sure all customs formalities are complied with. The minimum quantity of plant material or seed to be supplied by the applicant in one or several samples should be:

Seed propagated varieties : 30g of seed Vegetatively propagated varieties : 500 bulblets or sets

The seeds submitted for testing must have at least 90% germination, utmost

10% moisture content and 99% purity.

The seed and plant material must not have undergone any treatment unless the PVPO requires such treatment. If it has been treated, full details of the treatment must be given.


A minimum of two tests will be required and these be conducted during the dry season at one place. If any important characteristics of the variety cannot be seen at that place, the variety may be tested at an additional location.

The tests should be carried out under conditions ensuring normal growth.

The size of the plots should be 1 x 5 m. or 10 sq. m. for the two replications following 20 cm x 20 cm planting distance. Each test should include a total of 250 plants. Separate plots for observation and for measuring can only be used if they have been subject to similar environmental conditions.



All observations determined by measurement or counting should be made on 60 plants or parts taken from each of 60 plants.

For the assessment of uniformity of vegetatively propagated varieties, a

population standard of 1% with an acceptance probability of at least 95% should be

Test Guidelines


128

applied. In the case of a sample size of 100 plants the maximum number of off-types allowed would be 3.

For the assessment of uniformity of open-pollinated and hybrid varieties relative uniformity standards should be applied.

All observations on the leaf and the foliage should be made on the fifth

leaf stage. All observations on the bulb should be made at harvest maturity.


The collection of varieties to be grown should be divided into groups to

facilitate the assessment of distinctness. Characteristics which are suitable for growing purposes are those which are know from experience not to vary, or to vary only slightly, within a variety. Their various stage of expression should be fairly distributed throughout the collection.

It is recommended that the following characteristics will be used for grouping

varieties:

Bulb: splitting into bulblets (with dry skin around each bulblet) (characteristic 10)

Bulb/Bulblet: general shape (in longitudinal section) (characteristic 18) Bulb/Bulblet: basic color for dry skin ( characteristic 23)


To assess distinctness, uniformity and stability, the characteristics and their states as given in the Table of Characteristics should be used. The name of each example variety is followed by an abbreviation indicating whether the variety is an onion (O) or a shallot (S).

Notes (numbers), for purposes of electronic data processing, are given

opposite the states of expression for each characteristic.

Legend:

(*) Characteristics that should be used on all varieties in every growing period over which the examinations are made and always be included in the variety descriptions, except when the state of expression of a preceding characteristics or regional environmental conditions render this impossible.

(+) See Explanations on the Table of Characteristics in Chapter VIII.

(1) The optimum stage of development for the assessment of each characteristic is indicated by a number in the second column. The stages of development denoted by each number are described at the end of the Chapter VIII.

(2) Type of example variety: O = Onion, S = Shallot.

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129


Trait No.

Characteristics


Reference (Example)Varities Note / Remarks Onion Shallot

1. (*)

Plant: number of leaves per pseudostem

few

3

medium 5

many

7

2 (*)

Foliage: orientation erect 1

semi-erect

2

horizontal

3

3 (*)

Foliage: waxiness

absent

1

medium

5

strong 9

4. (*)

Foliage: green color

light 3

medium

5

dark 7

5. (* ) (+)

Foliage: cranking

absent or very weak

1

weak

3

medium

5

strong 7

very strong 9

6.1 Onion varieties only: Leaf: length

very short 1

short

3

medium 5

long

7

very long 9

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130

Trait No.

Characteristics


Reference (Example)Varities Note / Remarks

Onion Shallot

6.2 Shallot varieties only: Leaf: length

very short 1

short

3

medium

5

long

7

very long 9

7.1 (*)

Onion varieties only: Leaf: diameter

small 3

medium

5

large

7

7.2 (*)

Shallot varieties only: Leaf: diameter

small 3

medium

5

large 7

8. (+)

Onion varieties only: Pseudostem: length (up to highest green leaf)

short 3

medium

5

long

7

9. (+)

Onion varieties only: Pseudostem: diameter (at midpoint of length)

small 3

medium

5

large

7

10.a (*) (+)

Bulb:splitting into bulblet (with dry skin around each bulblet)

absent 1

present

9

10.b (*)

Bulb: internal split absent 1

present

9

11 (*)

Shallot varieties only: Bulb: degree of splitting into bulblet

weak (1-2) 3

medium (3-4) 5

strong ( more than 5)

7

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131

Trait No.

Characteristics



12.a (*)

Onion varieties only: Bulb: size

small 3

medium

5

large

7

12.b (*)

Shallot varieties grown from bulblets only: Bulblet: size

small 3

medium

5

large

7

13.a (*)

Onion varieties only: bulb Polar diameter

short 3

medium

5

tall

7

13.b (*)

Shallot varieties grown from bulblets only: Bulblet: polar diameter

short 3

medium

5

tall 7

14.a (*)

Onion varieties only: Bulb: equatorial diameter

small 3

medium

5

large 7

14.b (*)

Shallot varieties grown from bulblet only: Bulblet: equatorial diameter

small 3

medium 5

large

7

15.a (*)

Onion varieties only: Bulb:ratio height/diameter

small 3

medium

5

large

7

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132

Trait No.

Characteristics



15.b (*)

Shallot varieties from Bulblet only: Bulblet: Ratio height/diameter

small 3

medium

5

large

7

16 (*) (+)

Bulb/Bulblet: position of maximum diameter

towards apex 1

at middle

2

towards base 3

17 (+)

Bulb/Bulblet: width of neck

narrow 3

medium 5

broad 7

18 (*) (+)

Bulb/Bulblet: general shape (in longitudinal section)

elliptic 1

ovate 2

broad elliptic 3

circular 4

broad ovate 5

broad obovate 6

rhombic 7

transverse elliptic

8

transverse narrow elliptic

9

19. (*) (+)

Onion varieties only: Bulb: shape of top (as for 18) Depressed

flat 1

slightly raised

2

rounded

3

slightly sloping 4

strongly sloping

5

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133

Trait No.

Characteristics



Remarks Onion Shallot

20. (*) (+)

Bulb/Bulblet: shape of base (as for 18)

recessed 1

flat

2

round 3

weakly tapered 4

strongly tapered

5

21. Bulb/Bulblet: adherence of dry skin after harvest

weak 3

medium

5

strong 7

22. Bulb/Bulblet: thickness of dry skin

thin 3

medium

5

thick 7

23. (*)

Bulb/Bulblet: basic color of dry skin

white 1

gray

2

green

3

yellow

4

brown

5

pink

6

red

7

24. (*)

Bulb/Bulblet: intensity of basic color of dry skin

light 3

medium

5

dark

7

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134

Trait No.

Characteristics




25. (*)

Bulb/Bulblet: hue of color of dry skin (in addition to basic color)

absent 1

grayish 2

greenish

3

yellowish 4

brownish

5

pinkish

6

reddish

7

purplish

8

26. (*)

Bulb/Bulblet: coloration of epidermis of fleshy scales

absent 1

greenish

2

reddish

3

27. (*) (+)

Bulb/Bulblet: number of axes

very few 1

few

3

medium

5

many 7

28. (+)

Bulb/Bulblet: dry matter content

very low 1

low

3

medium

5

high

7

very high 9

29. Onion: tendency to bolt

absent or very weak

1

weak

3

medium

5

strong

7

very strong

9

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135

Trait No.

Characteristics




30. Onion varieties only: time of beginning of bolting

early 3

medium 5

late

7

31.a Onion varieties only: time of harvest maturity

early 3

medium

5

late 7

31.b Shallot varieties only: time of harvest maturity

early Australia, Farmer‟s Batanes

3

medium Tanduyong 5

late Batanes 7

32. (*)

Time of sprouting during storage

early Batanes 3

medium

Tanduyong

5

late Red Pinoy, Nueventa

Farmer‟s Batanes

7

33. Reaction to Pest

33.1 Purple blotch susceptible Red Pinoy, Nueventa

Farmer‟s Batanes

resistant

6 S (Sumenep)

33.2 Cercospora leaf spot

susceptible

Red Pinoy, Nueventa

Farmer‟s Batanes

resistant

33.3

Bulb rot susceptible Red Pinoy, Nueventa

Farmer‟s Batanes

resistant

33.4 Anthracnose susceptible

Red Pinoy, Nueventa

Farmer‟s Batanes

resistant

33.5 Nematode

susceptible

Red Pinoy, Nueventa

Farmer‟s Batanes

resistant

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136


Trait No. 5 Foliage : cranking

Trait No. 8 and 9 Onion varieties only : pseudostem : length (up to highest green leaf) (8) and diameter (at midpoint of length) (9)

Trait No. 10 Bulb : splitting into bulblets (with dry skin around each bulblet)

1 absent

or very weak

3 weak

5 medium

7 strong

9 very strong

1 absent

9 present

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137

Trait No. 16 Bulb/Bulblet : position of maximum diameter

Trait No. 17 Bulb/Bulblet : width of neck

Trait No. 18 Bulb/Bulblet : general shape ( in longitudinal section)

1 towards apex

2 at middle

3 towards base

1 very narrow

3 narrow

5 medium

7 broad

9 very broad

1 elliptic

2 ovate

3 broad elliptic

4 circular

5

broad ovate

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138

Ad. 19 Onion varieties only : Bulb : shape of top (as for 18)

Ad. 20 Bulb/Bulblet : shape of base (as for 18)

6 broad

obovate

7 rhombic

8

tranverse elliptic

9 tranverse

narrow elliptic

2 flat

1 depressed

3

slightly raised

4 rounded

5 slightly sloping

6

strongly sloping

1 recessed

2

flat flat

3 round

4

weakly tapered

5 strongly tapered

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139

Ad. 27 Bulb/Bulblet: number of axes The number of axes should be assessed when the bulb/bulblet has completely died back and before there is any sprouting. The bulb or bulblet should be cut in transverse section, starting at the widest point and make further cuts towards the base of the bulb/bulblet, until there is consistency in the number of axes. Each axis appears as a point, often greenish in color surrounded by tissue rings. Ad. 28 Bulb/Bulblet: dry matter content Dry matter content should be determined according to IV-I for 3 x 20 bulbs (e.g. one sample of 20 bulbs from each plot). From these bulbs the dry skin should be prepared by cutting the bulbs into small pieces of 1-5 mm size. A representative sample should be weighed directly after cutting ( we should be aware that the biodegradation of sugars and carbohydrates starts as soon as cells are damaged). The sample should be dried for 2 hours at 1050C and then the temperature should be lowered to 650C during 22 hours. Lowering of temperature is necessary to avoid caramelisation. The remaining weight should be assed after 24 hours. From these figures the dry matter content may be calculated. The dry matter content could also be assessed by obtaining the total soluble solids (TSS) using a refractometer.

Ad. 35 Time of sprouting during storage

Care should be taken to exclude damaged bulbs. Storage temperature should be maintained under ambient condition. Code for the Growth Stages 1 : Seed to Bulb Growth stage / General description

Vegetative Cycle

00 Dry

0 Germination

Seedling growth

10 Emerged seedling at „loop‟ stage

15 Seedling with testa above ground and still attached to cotyledon

20 Emergence of first true leaf 25 Second true leaf stage

30 Third leaf stage

35 Fourth leaf stage

40 Fifth leaf stage

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140

Plant Growth Sixth leaf stage

50 Seventh leaf stage-first leaf senescing 55 Eighth leaf stage 60 65 Tenth leaf stage; second and third leaf senescing; early bulb development 70 75 80 85 90 95

100 Full expansion of leaves achieved; continued swelling of bulb 105 Beginning of foliage fall-over, weakening of false stem turgidity 115 Leaves dry off; bulb size continues to increase; darkening of bulb scales 120 125 130 135 Bulb at harvestable maturity 140 145 150 Complete die-back of foliage; bulb top seals over for dormancy

Reproductive Cycle Start of sprouting in store – swelling of root initials or emergence of shoot at top of bulb

170.1 180.1 Sprouted bulbs with emerging leaves 190.1 200.1 Splitting of outer skin 210.1 Emergence of scape and underdeveloped spathe 220.1 Scape elongation and swelling of middle 230.1 240.1 Swelling of spathe 250.1 Splitting of spade 260.1 Enlargement of umbel 270.1 Opening of florets (where fertile) 280.1 Pollination of florets 290.1 Seed set – swelling of pollinated ovaries 300.1 310.1 320.1 Ripe seed in umbels 330.1

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141

Dry seed

150 Dry bulb with complete foliage die-back 160.2 170.2 Bulb shape becoming less rounded 180.2 190.2 Bulb shape becoming irregular with development of small splits in the dry outer skin 200.2 210.2 More than one growing point emerging from the top of the bulb 220.2 Long splits developing in the outer skin of the bulb and differentiation of bulb into bulblets 240.2 250.2 260.2 270.2 Separation ob bulblets from parent bulb except at base. Bulblets

separated from each other by a dry outer skin 280.2 290.2 Complete separation of bulblets from „parent bulb‟ 300.2 Development of many leaves

Literature BREWSTER, J. L., 1994: “Crop Production Science in Horticulture 3: Onions and Other

Vegetables Alliums.” CAB International. BREWSTER, J. L. and BARNES, A., 1981: “A Comparison of Relative Growth Rates of

Different Individual Plants and Different Cultivars of Onion of Diverse Geographic Origin at Two Temperatures and Two Light Intensities.” Journal of Applied Ecology Vol. 18, 589-604.

BREWSTER, J. L., SALTER, P. J. and DARBY, R. J., 1977: “ Analysis of the Growth and

Yield of Over-wintered Onions.” Journal of Horticulture Science Vol. 52, 335-346. CLARKE, A. E., JONES, H. A. and LITTLE, T. M., 1994: “Inheritance of Bulb Colour in the

Onion.” Genetics 29, pp 569-575. EL-SHAFIE, M. W. and DAVIES, G. N., 1967: “ Inheritance of Bulb Color in the Onion

(Allium cepa L.).” Hilgardia Vol. 38, No. 17, 607-622. JONES, H. A., CLARKE, A. E. and STEVENSON, F. J., 1944: “Studies in the Genetics of

the Onion (Allium cepa L.).” Proceedings of the American Society for Horticulture Science 44, pp 479-484.

JONES, H. A. and MANN, L. K., 1963: “onions and Their Allies: Botany, Cultivation and

Utilisation.” London, Leonard Hill. JONES, H. A. and PETERSON, C. E., 1952: “Complementary Factors for Light-Red Bulb

Colour in Onions.” Proceedings of the American Society for Horticulture Science Vol. 59, 457.

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142

KAPPERT and RUDOLF, W., 1962: “Züchtung von Gemüse, Obst, Reben and Forstpflanzen.” Verlag Paul Parey, Berlin und Hamburg, pp 270-312.

KUCKUCK, H. and KOBAKE, G., 1962, in ROEMER, T. and RUDOLF, W., 1962: “Handbuch der Pfanzen-Züchtung, Band VI.” Verlag Paul Parey, Berlin und Hamburg.

MAGRUDER, R. and ALLARD, H. A., 1937: “Bulb Formation in Some American and

European Varieties of Onions as Affected by Length of Day.” Journal of Agricultural Research Vol. 54, Part No. 10, 719-752.

MAGRUDER, R. et al, 1941: “Descriptions and Types of Principal American Varieties of

Onion.” USDA, Miscellaneous Publication No. 435, Washington DC. MESSIAEN, C. M., COHAT, L., LEROUX, J. P., PICHON, M., BEYRIES, A., 1993: “Les

allium alimentaires reproduits para voie végétative.” INRA Editions, Paris. MIDMORE, D. J., 1994, (Editor): “International Symposium on Alliums for the Tropics.”

Acta Horticulturae, 358. RABINOWITCH, H. D. and BREWSTER, J. L., 1990: “ Onions and Allied Crops.” Vol. 1-3

CRC Press, Boca Raton. REIEMAN, G. H., 1931: “Genetic Factors for Pigmentation in the Onion and Their

Relation to Disease Resistance.” Journal of Agricultural Research Vol. 42, No. 5, 251-278. SCULLY, N. J., PARKER, M. W. and BORTHWICK, H. A. 1945: “Interaction of Nitrogen

Nutrition and Photo-period as Expressed in Bulbing and Flower Stalk Development of Onion.” Botanical Gazette Vol. 107, 52-61.

SCWARTZ, H. F. and MOHAN, S. K., 1995 (Editors): “Compendium of Onion and Garlic

Diseases.” The American Phytopathological Society, (ISBN: 0-89-54-170-1). WRIGHT, C. J. and SOBEIGH, W. Y., 1986: “The Photo-periodic Regulation of Bulbing in

Onions (Allium cepa L.), Effects of Irradiance.” Journal of Horticulture Science Vol. 61, Part 3, 311-335.

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143

X. Technical Questionnaire Reference Number

(not to be filled in by the applicant) _____________________________

_____________________________

TECHNICAL QUESTIONAIRE to be completed in connection with an application for plant breeders‟ rights

1. Species Allium cepa L. Allium escalonicum L. (a) ONION ( ) (b) SHALLOT ( )



4. Information on origin, maintenance and reproduction of the variety 4.1 Variety type (a) open-pollinated ( ) (b) single hybrid ( ) (c) three-way hybrid ( ) (d) clone ( ) (e) other type (indicate what type) ( ) Other information

Test Guidelines


144

5. Characteristics of the variety to be indicated ( the number in brackets refers to the corresponding characteristic in Test Guidelines; please mark the state of expression which best corresponds).


Example Varieties

Note

5.1 (1)

Plant: number of leaves per pseudostem

few

medium

many

3 [ ]

5 [ ]

7 [ ]

5.2 (4)

Foliage: green color

light

medium

dark

3 [ ]

5 [ ]

7 [ ]

5.3a (10)

Bulb: splitting into bulblets (with dry skin around each bulblet)

absent

present

1 [ ]

9 [ ]

5.3b Bulb: internal split

absent

present

1 [ ]

9 [ ]

5.4.1 (12.1)

Onion varieties only: Bulb: size

small

medium

large

3 [ ]

5 [ ]

7 [ ]

5.4.2 (12.2)

Shallow varieties grown from blblets only: Bulblet: size

small

medium

large

3 [ ]

5 [ ]

7 [ ]

5.5 (18)

Bulb/Bulblet: general shape (in longitudinal section)

elliptic

ovate

broad elliptic

circular

broad ovate

broad obovate

rhombic

transverse elliptic

transverse narrow elliptic

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

6 [ ]

7 [ ]

8 [ ]

9 [ ]

Test Guidelines


145

Characteristics Description/Category Example Varieties

Note

5.6 (23)

Bulb/Bulblet: basic color of dry skin

white

grey

green

yellow

brown

pink

red

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

6 [ ]

7 [ ]

5.7 (25)

Bulb/bulblet: hue of color of dry skin

absent

grayish

greenish

yellowish

brownish

pinkish

reddish

purplish

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

6 [ ]

7 [ ]

8 [ ]

5.8 (33)

Onion varieties only: Time of harvest maturity

very early

early

medium

late

very late

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

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6. Similar varieties and differences between these varieties


Characteristic in which the similar variety is different o)



o) In the case of identical states of expressions of both varieties, please indicate the size of the difference.


7.1 Resistance to pests and diseases 7.2 Special conditions for testing the variety

7.2.1 Day length conditions which favor full bulb development (a) short day (b) long day

[ ] [ ]

7.2.2 Dry matter content (a) low (b) medium (c) high

[ ] [ ] [ ]

Suitability for storage (a) none (b) short term (c) long term

[ ] [ ] [ ]

Other information

8. Authorization for release

(a) Does the variety require prior authorization for release under legislation concerning the protection of the environment, human and animal health? Yes [ ] No [ ] (b) Has such authorization been obtained? Yes [ ] No [ ] If the answer to that question is yes, please attach a copy of such an authorization. end of document

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Tomato (Lycopersicon esculentum Mill.) I. Coverage of These Guidelines

The Test of Guidelines described in this publication for the conduct of distinctness, uniformity and stability tests (DUS) shall apply to all varieties of tomato (Lycopersicon esculentum Mill. syn Lycopersicon lycopersicon (L.) Karstenex Farw.).

II. Plant Propagation Material Requirement

Upon application, 3,000 viable and high quality seeds are to be submitted to the Plant Variety Protection Office (PVPO) together with the application. Applicants outside the Philippines must make sure that all custom formalities regarding seed exportation/importation are complied with.

The PVP office will determine the additional amount of seeds to be submitted to

the PVP office for the substantive examinations or comparative growing trial. The minimum requirement, however, is 10g or 3,000. Seeds must have at least 90% germination, 99% purity, at most 10% moisture content, vigorous, and free from pest or disease

The seeds should not have undergone any form of treatment unless otherwise

allowed or requested by the PVP Board in certain circumstances. In such cases, the details of the seed treatment must be clearly described and declared.


The minimum duration of the DUS test should be at least two similar growing periods i.e. two dry or two wet seasons. The test should be conducted in at least one location or at least in one accredited DUS Testing Center to be determined by the PVP Board.

The comparative growing trial must be carried out under conditions that would

ensure normal growth of the plants. The size of the plots should be such that plants or parts of plants may be removed for measurement and counting without prejudice to the observations which must be made up to the end of the growing period. Each trial or test should have at least two replications. The minimum total number of plants to be tested for each comparative growing trial is 20 plants for greenhouse evaluation and 40 plants for open field trials. Separate plots for observation and for measurement purposes can only be used if the growing and environmental conditions are similar.

All test varieties must be treated uniformly i.e. water or irrigation, insecticide,

fungicide, and others following the requirement of National Cooperative Trials (NCT) cultural management recommendations.

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Unless otherwise indicated, all observations determined by measurement, weighing or counting should be made on 20 plants or parts taken from each of 20 plants.

For the assessment of uniformity, a population standard of one percent (1%) and

an acceptance probability of at least ninety-five (95%) should be applied. In the case of a sample size of 20 plants, the maximum number of off-types allowed would be one (1) In the case of a sample size of 40 plants, the maximum number of off-types would be two (2).

When the resistance characteristics are used for assessing distinctness, uniformity

and stability, records must be taken under conditions of controlled infection and, unless otherwise specified, on at least 10 plants.

All observations on the leaf should be made before ripening of the first fruit

cluster. V. Grouping of Varieties

To facilitate the assessment of distinctness, the varieties for evaluation should be divided into groups. Characteristics which are suitable for grouping purposes are those with are known from experience not to vary, or to vary only slightly, within a variety. Their various states of expression should be fairly evenly distributed throughout the collection.

It is recommended that the following characteristics be used for grouping

varieties:

Plant: growth type (characteristic 2) Maturity (duration of harvest)


The test for distinctness, uniformity and stability should be based on the characteristics and their state of expressions as listed in the Table of Characteristics (next chapter).

For the purpose of electronic data processing, notes (numbers) are given opposite

the states of the different characteristics. Following are the legend/description of the symbols used in the Table of Characteristics:

(*) refers to characteristics that should be used on all varieties in every growing period over which examinations are made and always included in the variety descriptions, except when the state of expression of a preceding characteristic or certain environmental condition would render this impossible

(+) See explanations on the Table of Characteristics in Chapter VIII.

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VII. Table of Characteristics for Tomato (Lycopersicon esculentum Mill.)

Trait No.

Characteristic Description/

Category Reference


Remarks

1. (*)

Seedling: anthocyanin coloration of hypocotyls

absent

1

present

9

2. (*) (+)

Plant: growth type determinate

1

indeterminate

2

3. Only determinate growth type varieties: Plant: number of inflorescences on main stem (sideshoots to be removed)

few

3

medium

5

many

7

4. (+)

Stem: anthocyanin coloration of upper third

absent or very weak

1

weak 3

medium 5

strong 7

very strong

9

5. (+)

Only indeterminate growth type of varieties: Stem: length of internode (between 1st and 4th inflorescence)

short

3

medium

5

long

7

6. (*)

Leaf: attitude/orientation in middle third of plant

semi-erect

3

horizontal

5

semi-drooping

7

7. (*)

Leaf: length (indicate range for each category)

short

3

medium

5

long

7

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Trait No. Characteristic

Description/ Category

Reference (Example) Varieties

Note/ Remarks

8. (*)

Leaf: width (indicate range for each category)

narrow

3

medium 5

broad

7

9. (*)

Leaf: division of blade pinnate

1

bipinnate

2

10. (+)

Leaf: size of leaflets (in middle of leaf)

very small

1

small

3

medium

5

large

7

very large

9

11. Leaf: intensity of green color

light

3

medium

5

dark

7

12. Leaf: glossiness (as for 6) weak

3

medium 5

strong

7

13a. Leaf: blistering (as for 6) absent

1

present

9

13b. Leaf: blistering if present (as for 6)

weak

3

medium

5

strong

7

14. Leaf: size of blisters (as for 6) small

3

medium

5

large

7

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Note/ Remarks

15. (+)

Leaf: attitude/orientation of petiole of leaflet in relation to main axis (as for 6)

semi-erect

3

horizontal

5

semi-drooping

7

16. Inflorescence: type (2nd and 3rd truss)

mainly uniparous

1

intermediate

2

mainly uniparous

3

17. Flower: fasciation (first flower of inflorescence)

absent (sessiled)

1

present (pediceled)

9

18. (+)

Flower: pubescence of style

absent or very scarce

1

present

9

19. (*)

Flower: color yellow

1

orange

2

20. (*) (+)

Peduncle: abscission layer

absent

1

present

9

21. (*) (+)

Only for varieties with abscission layers Peduncle: length (from abscission layer to calyx)

short

3

medium 5

long

7

22. (*)

Fruit: size (individual fruit weight in grams)

very small

1

small

3

medium

5

large

7

very large

9

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Trait No.

Characteristic Description/ Category


Note/ Remarks

23. (*)

Fruit: ratio Length/diameter

very small

1

small

3

medium

5

large

7

very large

9

24. (*) (+)

Fruit: shape in longitudinal section

flattened

1

slightly flattened

2

circular

3

rectangular

4

cylindrical

5

elliptic

6

heart-shaped

7

obovate

8

ovate

9

pear-shaped

10

25. (*)

Fruit: ribbing at peduncle end absent or very weak

1

weak

3

medium

5

strong

7

very strong

9

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Note/ Remarks

26. Fruit: cross section not round

1

round

2

medium

5

large 7

very large

9

27. (+)

Fruit: depression at peduncle end

absent or very weak

1

weak

3

medium

5

strong

7

very strong

9

28. Fruit: size of peduncle scar

very small

1

small

3

medium

5

large

7

very large

9

29. Fruit: size of blossom scar

very small 1

small

3

medium

5

large

7

very large

9

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154

Trait No.

Characteristic Description/

Category


Note/ Remarks

30. (+)

Fruit: shape at blossom end

Indented

1

indented to flat

2

flat

3

flat to pointed

4

pointed (Slight)

5

pointed (Medium)

7

highly Pointed

9

31. Fruit: size of core in cross section (in relation to total diameter)

very small

1

Small

3

Medium

5

Large

7

very large

9

32. Fruit: thickness of pericarp

Thin

3

medium

5

thick

7

33. (*)

Fruit: number of locules only two

1

two or three

2

three or four

3

four, five or six

4

more than six

5

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Note/ Remarks

34. (*)

Fruit: green shoulder before maturity

absent 1

present

9

35. (*)

Fruit: extent of green shoulder (as for 34)

small

3

medium

5

large

7

36. (*)

Fruit: intensity of green color of shoulder (as for 34)

light

3

medium

5

dark

7

37. (*)

Fruit: intensity of green color (as for 34)

light

3

medium

5

dark

7

38. (*)

Fruit: color at maturity (vine ripe)

cream

1

yellow

2

orange

3

pink

4

red

5

brownish

6

39. (*)

Fruit: color of flesh (at maturity) (vine ripe)

cream

1

yellow

2

orange

3

pink

4

red

5

brownish

6

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Note/ Remarks

40. (*) (+)

Fruit: firmness

very soft

1

soft

3

medium

5

firm

7

very firm

9

41. (+)

Fruit: shelf life (under ambient condition; average no of days from harvest to 50% of the fruits still marketable)

very short

1

short

3

medium

5

long

7

very long

9

42. (+)

Time of flowering (from sowing)

early

3

medium

5

late

7

43. (*)

Time of maturity (from sowing)

very early

1

early

3

medium

5

late

7

very late

9

44. Fruit: Total soluble Solid at maturity

low

3

medium

5

high

7

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Note/ Remarks

45. Fruit: pH of ripe fruit low

3

medium

5

high

7

46. Fruit: glossiness (mature green stage)

weak

3

medium 5

strong 7

47. Fruit: number/cluster (from 3rd truss)

few

3

intermediate

5

many

7

48. Fruit: gel (vine ripe) yellow

1

orange

2

red

3

green

4

49. Fruit size uniformity/cluster (from 3rd truss)

variable (<49% fruits are of same

size

3

moderately uniform (50-

89% fruits are of same size)

5

highly uniform (at least 90%

fruits are of same size)

7

50. Fruit: cracking absent

1

concentric

2

radial 3

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Note/ Remarks

50.1

Fruit: degree of cracking

slight

1

medium

3

medium to severe

4

severe

5

51 Fruit: blotchy ripening

absent

1

present

9

52. (+)

Sensitivity to silvering insensitive

1

sensitive

9

53. (+)

Resistance to nematode (Meloidogyne incognita)

absent

1

present

9

54 (+)

Resistance to Fusarium oxysporum f. sp. lycoperscici

54.1 (*)

Race 0 (ex 1)

absent

1

present

9

54.2 (*)

Race 1 (ex 2) absent

1

present

9

55. (+)

Resistance to Fusarium oxysporum f. sp. radicis lycoperscici

absent

1

present 9

56. (+)

Resistance to leaf mold (Cercospora fuligena)

absent

1

present

9

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Note/ Remarks

57. (+)

Resistance to Tomato Mosaic Virus

no virus infection (absent = highly

resistance)

0

1-10% virus infection

(resistant)

1

11-20% virus infection)

(moderately resistant)

2


(moderately susceptible)

3


(susceptible)

4

76-100% virus infection (highly

susceptible)

5

58. (+)

Resistance to late blight (Phytophthora infestans)

no symptom (absent = highly

resistant)

0

1-10% of leaf area affected

(highly resistant)

1

11-20% of leaf area affected (moderately

resistant)

2


(resistant)

3

41-70 of leaf area affected


4

71-90% of leaf area affected (susceptible)

5


(highly susceptible)

6

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Trait No.



Note/ Remarks

59. (+)

Resistance to gray leaf spot (Stemphylium spp.)

absent

1

present

9

60. (+)

Resistance to Bacterial Leaf Spot (Pseudomonas syringe pv. tomato)

absent

1

present 9

61. (+)

Resistance to Ralstonia solanacearum – Race 1

80-100% survival (resistant)

1

60-79% survival (moderately

resistant)

2

30-59% survival (moderately susceptible)

3

0-29% survival (susceptible)

4

62. (+)

Resistance to Tomato Yellow Leaf Curl Virus

no virus infection (absent=highly

resistant)

0


(resistant)

1


(moderately resistant)

2



3


(susceptible)

4

76-100% virus infection (highly

susceptible)

5

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161

Trait No.



Note/ Remarks

63. (+)

Resistance to Leveilulla taurica

absent

1

present

9

64. (+)

Resistant to Oidium Lycopersicon

absent

1

present

9

65. (+)

Resistance to bacterial spot (Xanthosmonas campestris pv. vesicatoria)

absent

1

present

9

66. (+)

Reaction to blossom end rot (BER)

absent

1

severe

9

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162

VIII. Explanation on the Table of Characteristics Trait No. 2 Plant: growth type The growth type is predominantly controlled by one monoallelic gene (self-pruning + / self pruning 1). Determinate (1): This type is predominantly controlled by the recessive allele, self-pruning – (Sp-). This type produces a limited number of trusses. The number of trusses is different among plants and is influenced by agroclimatic conditions. In this type, the number of leaves or internodes between inflorescences varies from one to three. In the terminal truss, the stem ends with an inflorescence and no lateral shoots are produced. This type includes some “semi-determinate” varieties which do not have three leaves or internodes consistently between inflorescences, and show semi-determinate growth, fro example, with the termination of the stem prolongation above 9th inflorescence (e.g. “Local Variety” type) or at higher than 20th inflorescence (e.g. “Local Variety” type). Indeterminate (2): This type is predominantly controlled by the dominant allele, self-pruning + (Sp+). In this type, three leaves or internodes are generally observed between inflorescence. Each tress produces three buds, the terminal bud is transformed into a flowering bud; one of the two axillary buds is transformed into a lateral shoot which produces next three buds and carries on the prolongation of the stem. Plants of this type grows with the continuous repeat of this growth pattern. It should be noted that only two leaves or internodes might be observed between inflorescences in some parts of plants in a certain group of indeterminate variety types (e.g. varieties originated from “Local Variety”. Local Varieties, types might be considered to be categorized into an intermediate class between indeterminate and determinate, but they always have three leaves or internodes between inflorescences. They should therefore be categorized into the indeterminate type. Trait No. 4 Stem: anthocyanin coloration of upper third Most of the varieties are classed 1 to 5. Expression of anthocyanin is influenced by day temperature. Under greenhouse conditions, the variation is rather low, except for varieties with Tm2 allele which is linked to anthocyanin of the stem (specially at the internode). Trait No. 5 Stem length of internode between 1st and 4th inflorescence (only for indeterminate growth type varieties) Indeterminate growth type varieties have, in general, 3 nodes between inflorescences except some genotypes (see Trait No. 2). This means that, in general, there are 12 internodes between the 1st and 4th inflorescence.

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Measure the length between the 1st and 4th inflorescence, and count the number of internodes (generally 12). In order to obtain the average length of an internode, calculate the ration: length of stem/number of internodes. The observation should be made at the following stage: one leaf above the 5th or 6th inflorescence on staked open field crops one leaf above the 7th to 12th inflorescence for plants grown in a greenhouse, depending on the height of the greenhouse Trait No. 10 Leaf size: size of leaflets (in middle of leaf) Trait No. 15 Leaf: attitude of petiole of leaflet in relation to main axis 3 5 7 semi-erect horizontal semi-drooping Trait No. 18 Flower: pubescence of style Some non-hairy varieties can present some rare and small hairs at the base of the style.

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Trait No. 20 Peduncle: abscission layer absent present Some varieties which have only a collar of an abscission layer (heterozygous for the gene which controls the presence of the joint) are considered as jointless (“absent (1)”). Trait No. 21 Only for varieties with abscission layer: peduncle: length (fro abscission layer to calyx). Trait No. 24 Fruit: shape in longitudinal section 1 2 3 flattened slightly flattened circular

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4 5 6 7 rectangular cylindrical elliptic heart -shaped 8 9 10 obovate ovate pear-shaped Trait No. 27 Fruit: depression at peduncle end 1 3 5 9 absent or very weak weak medium strong

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Trait No. 30 Fruit: shape at blossom end

1 2 3 4 5 indented indented to flat flat flat to pointed pointed

Trait No. 40 Fruit Firmness Method: Harvesting stage: fruits should be harvested when they are completely colored. Determining firmness: determine by hand the firmness of the fruits compared to the standard varieties. Trait No. 41 Fruit: shelf-life The length of shelf life is estimated by the number of weeks or days that the fruit remains commercially viable on the shelf. Twenty fruits per plot (2 per plant) are picked from the 4th, 5th or 6th cluster in similar stages of exterior ripening (when green color disappears in half of whole fruit). Fruits are stored in boxes in stackable plastic trays in single layers. The boxes can be stored one on top of another if they permit the air to circulate between them. The storage place must be at ambient condition under low elevation. Observation is made twice a week based on firmness, shivering, and rotting of fruits until 50% of fruits are non-marketable. A fruit is considered no longer marketable if the rating for softness is lower or equal to “3” (see characteristic number 40 in chapter 7). The length of shelf life is calculated as the number of days from picking to the time when the fruit firmness is no longer marketable.

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Trait No. 42 Time of Flowering and Maturity For semi-indeterminate or indeterminate varieties that need stakes or trellis, the time to flowering and maturity is assessed by recording the flowering date and first harvest from sowing of the third flower on the second and third trusses on individual plants. It is not recommended to record the time of flowering in the first trusses since its expression of flowering is more influenced by the seed vigor and the plantation quality. Obtain the average of the number of days to flowering. For determinate varieties, it is strongly advisable to grow them with stakes or trellis and to record the flowering date in the same manner described above. Trait No. 44 and 45 Fruit: Total Soluble Solids and Ph The total soluble solids of freshly squeezed juice from 20 fruits ripe fruits will be taken using a hand refractometer and recorded as percent degree Brix. The pH will be taken using a pH meter. Trait No. 46 to 60 Resistance to Various Diseases The protocol used for disease screening and evaluation for resistance (either seeding or field-grown) must be fully described. As much as possible, use known varieties with known level of resistance and susceptibility as check varieties. The isolate used in the screening or evaluation must also be described. For seeding inoculation/screening, sterilized soil mix must be used and conditions for disease development must be recorded for each screening period particularly if the screening could not be done under controlled conditions such as in the use of growth chambers. If evaluation or screening is done in multiple sites or locations, the date must for each location must be summarized and presented in relation to the check varieties as well as the environmental conditions during the screening. IX. Literature: - UPOV TG/44/10. 2001. Guidelines for the conduct of tests for distinctness, uniformity and stability for tomato (Lycopersicon lycopersicum (L.) Karsten ex. Farw.). International Union for the Protection of New Varieties of Plants (UPOV), 49 p. - AG:ABPGR/80/103. 1981. Genetic Resources of Tomatoes and Wild Relatives: A Global report by JT Esquinas-Alcazar. International Board for Plant Genetic Resources (IBPGR), 65 p.

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TECHNICAL QUESTIONNAIRE (To be completed in connection with an application for plant variety protection)

1. Species: Lycopersicon esculentum Mill TOMATO

2. Name and address of applicant:

3. Proposed denomination or breeders reference:

4. Information on origin, maintenance and reproduction of the variety Method of breeding/type of material : (i) seed propagagation - hybrid - open-pollinated Other information

[ ] [ ] [ ]

5. Characteristics of the variety to be given (the number in brackets refers to the corresponding characteristics in the DUS Test Guidelines and please mark the state of expression which best corresponds).

Trait No.



Remarks

5.1 (2)

Plant: growth type determinate

indeterminate

1 [ ]

2 [ ]

5.2 (9)

Leaf: division of blade pinnate

bipinnate

1 [ ]

2 [ ]

Reference Number : (not to be filled in by the applicant)

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169

Trait No.



Remarks

5.3 (20)

Peduncle: abscission layer absent

present

1 [ ]

9 [ ]

5.4 (22)

Fruit: size very small

small

medium

large

very large

1 [ ]

3 [ ]

5 [ ]

7 [ ]

9 [ ]

5.5 (24)

Fruit: length in longitudinal section

flattened

slightly flattened

circular

rectangular

cylindrical

elliptic

heart-shaped

obovate

ovate

pear-shaped

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

6 [ ]

7 [ ]

8 [ ]

9 [ ]

10 [ ]

5.7 (33)

Fruit: number of locules only two

two or three

three or four

four, five or six

more than six

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

5.8 (34)

Fruit: green shoulder before maturity

absent

present

1 [ ]

9 [ ]

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Trait No. Characteristic Description/Category


Note/ Remarks

5.9 (38)

Fruit: color at maturity cream

yellow

orange

pink

red

brownish

1 [ ]

2 [ ]

3 [ ]

4 [ ]

5 [ ]

6 [ ]

5.10 (40)

Fruit: firmness very soft

soft

medium

firm

very firm

1 [ ]

3 [ ]

5 [ ]

7 [ ]

9 [ ]

Similar varieties and differences from these varieties

Denomination of similar variety1

Characteristic in which the similar

variety is different2



1 Check variety from which the comparison was made 2 In the case of identical states of expression of both varieties, please indicate the size of the difference.

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171

7. Additional information which may help to distinguish the variety Resistance to pests and diseases

Organism Strain Absent Present Not tested

(53) Root knot nematode (Meloidogyne incognita) [ ] [ ] [ ] (54) Resistance to Fusarium oxysporum f. sp. lycopescici

[ ] [ ] [ ]

(55) Resistance to Fusarium oxysporum f. sp. radicts lycoperscici

[ ] [ ] [ ]

(56) Resistance to leaf mold (Cercospora fuligena) [ ] [ ] [ ] (57) Tomato Mosaic Virus [ ] [ ] [ ] (58) Late blight (Phythophthora infestans) [ ] [ ] [ ] (59) Gray leaf spot (Stemphylium spp) [ ] [ ] [ ] (60) Bacterial leaf spot (Pseudomonas syringae pv. Tomato)

[ ] [ ] [ ]

(61) Bacterial wilt (Ralstonia solanacearum) [ ] [ ] [ ] (62) Tomato Yellow Leaf Curl Virus Race 1 [ ] [ ] [ ] (63) Leveilulla taurica [ ] [ ] [ ] (64) Oidium lycopersicum [ ] [ ] [ ] (65) Bacterial spot (Xanthomonas campestris pv.vVesicatoria)

[ ] [ ] [ ]

(66) Blossom end rot [ ] [ ] [ ] [ ] [ ] [ ] Others (Please specify)

[ ] [ ] [ ]

Special conditions for the examination of the variety Type of culture - in the greenhouse [ ] - in the open [ ] - with trellis [ ] - without trellis [ ] Main Use - Fresh market or table [ ] - Industrial (please specify) or processing [ ] - Pot plant [ ] Other conditions Other information

[end of document]

Test Guidelines

PLANT VARIETY PROTECTION OFFICE 2nd Floor, National Seed Quality Control Services Building Bureau of Plant Industry Visayas Avenue, Diliman, Quezon City Telefax: (02) 929-2543 e-mail address: [email protected]

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