PROJECT OBJECTIVES & OVERVIEW

PROJECT DESCRIPTION, EXPERIMENTS & RESULTS

HUMAN PRACTICE PROJECT

UNDERGRADUATE STUDENTS:

1. Emmanuel Garuz2. Fotis Stringos3. Alicia Racine4. Miguel Cardenas5. Salomon Mitre6. Valery Diaz 7. Naicolette A. Muñoz 8. Jose Ng9. Arturo Nieves10.Dukakis Tejada11.Kesia Barrows12.Michelle Camarena

INSTRUCTOR & DIRECTORGrimaldo Elías Ureña M.

HUMAN PRACTICE ADVISORLucia L. Palma

UTP-PANAMA TEAM 2011

• Agricultural, Food & Civil-

Environmental Engineering

• Mechanical and Material Sciences

Engineering

• Electrical & Computational Engineering

Engineering tools obtained from biological resources

SYNBIO ENGINEERING TOOL KIT

Design of powerful sensors

Improvement of organisms ability to survive under temperature changes

Focus on environmental and climatic issues.

TEAM MAIN OBJECTIVES

Applications

PROJECT OBJECTIVES & OVERVIEW

PROJECT DESCRIPTION, EXPERIMENTS & RESULTS

HUMAN PRACTICE PROJECT

“THE RENBO” (THERMOGENIC RESPONSE NUTRIENT BIOSENSOR)

• Nitrates biosensor

(BioBrick

BBa_K381001)

• AOX periplasmatic

heat generator

(BioBrick

BBa_K410000)

PROJECT DESCRIPTION

Make Competent Cells using JM109Test our Competent CellsGet Familiarized with Protocols • New England Bio Labs• Open Wet Ware

• Reagents and buffer preparation

• Transformation• Mini Prep• Electrophoresis• Digestion • Ligation

LAB WORK

Test operation using 40mM KNO₃(if needed) under different:

• Substrates:• LB• Minimal essential medium• Saline solution

• Temperatures:• 8°C• 23°C• 37°C

Improve the characterization of pre-existing BioBricks

EXPERIMENT DESIGN OBJECTIVES

LB MEM SS

0.015

0.021

0.0050.006

0.004

0.014

0.000

0.006

OPTICAL DENSITY AT 8°C AND 420nm

BBa_K381001 BBa_K410000 BBa_K672000

LB MEM SS

0.599

0.341

0.010

0.258 0.275

0.015

0.138

0.0210.002

OPTICAL DENSITY AT 23°C AND 420nm

BBa_K381001 BBa_K410000 BBa_K672000

LB MEM SS

0.961

0.351

0.006

0.624

0.028

-0.001

0.742

0.006 0.003

OPTICAL DENSITY AT 37°C AND 420nm

BBa_K381001 BBa_K410000 BBa_K672000

LB MEM

0.225

0.005

Optical Density at 23°C and 534nmBBa_K381001

LB MEM

0.483

0.136

Optical Density at 37°C and 534nm

BBa_K381001

CONCLUSION

Determining the experimental conditions for optimal results for The Renbo.

• Best growth in LB @23°C

Improving characterization of pre-existing BioBricks

• New results for BBa_K381001 and BBa_k410000 in different substrates and temperatures.

• The PyeaR promoter showed a better activity over the HybB promoter.

• CspA promoter

(BBa_K328001)

• OmpA + AoX enzyme

• Nitrates biosensor

(BBa_K381001)

FUTURE DEVELOPMENTS

PROJECT OBJECTIVES & OVERVIEW

PROJECT DESCRIPTION, EXPERIMENTS & RESULTS

HUMAN PRACTICE PROJECT

1. How to introduce Synthetic Biology into our society.

2. Be able to find all the availability of institutions and the clear regulatory frame to develop our project.

1. The development of an outreach campaign about synthetic biology basic topics for every kind of public.

2. The development of a interactive software introducing the basics of synthetic biology and the iGEM competition.

3. The development of a guidance software that assists the user in the execution of projects related with synthetic biology.

PROBLEMS

OBJECTIVES

Synthetic Biology Tour with brochures, visuals, speeches and surveys.

OUTREACH

SURVEY’S RESULTS

Our team is also part of a new project called “Community Bricks”, a post iGEM group, that will be released this year. We are also participating in the set up of the Alumni iGEM.

SB UTP 1.0 is an interactive and educational program that introduces Synthetic Biology and iGEM to the users.

This software is available on Visual Basic 2010.This software is available on Visual Basic 2010.

SB UTP 1.0

SB UTP PROJECT 1.0 is a guidance program that helps groups of people with the process of implementing a Synthetic Biology project in their country.

SB UTP PROJECT 1.0

Our future objective for this software is that it will end as a data base, so that teams of any part of the world could use it and also add more information.

This software is available.

At the end of every question of the program, you will find a website or a paper as reference about the topic.

We exhort all of the future teams to complement this software.

SPONSORS

ASSOCIATE COLLABORATER