The Microscope

Compound MicroscopeCompound Microscope• Robert Hooke – 1665Robert Hooke – 1665

• Piece of corkPiece of cork

–Observation – cells Observation – cells are filled with juicesare filled with juices

Anton Van Anton Van LeeuwenhoekLeeuwenhoek

• 1670 – pond water1670 – pond water

• Discovered animalculesDiscovered animalcules–Now called microorganismsNow called microorganisms

Two Types of Two Types of MicroscopesMicroscopes

• Light Light –Glass lens and light raysGlass lens and light rays

• Electron Electron –Electrons instead of lightElectrons instead of light

PartsParts• ArmArm• BaseBase• OcularsOculars• Objectives Objectives • StageStage• Coarse AdjustmentCoarse Adjustment• Fine AdjustmentFine Adjustment• Light SourceLight Source

Procedures for Procedures for TransportTransport

• Two hands!!!!Two hands!!!!–ArmArm

–Base Base

Using the MicroscopeUsing the Microscope• Start on 4x scanning objective!Start on 4x scanning objective!

• Use Coarse Adjustment Use Coarse Adjustment

• After focus you can move up in After focus you can move up in magnification to 10Xmagnification to 10X

• Use Fine adjustment Use Fine adjustment

Storing MicroscopesStoring Microscopes• Turn off light sourceTurn off light source

• Wrap cord around bottom of armWrap cord around bottom of arm

• Lower stageLower stage

• 4X objective4X objective

• Cover Cover

MagnificationMagnification

• To determine your magnification…you just To determine your magnification…you just multiply the ocular lens by the objective multiply the ocular lens by the objective lenslens

• Ocular 10x Objective 40x:10 x 40 = 400Ocular 10x Objective 40x:10 x 40 = 400

Objective Lens have their magnificationwritten on them.

Ocular lenses usually magnifies by 10x

So the object is 400 times “larger”