supplemental figure s1. coleorhiza anatomy
DESCRIPTION
Supplemental Figure S1. Supplemental Figure S1. Coleorhiza anatomy. - PowerPoint PPT PresentationTRANSCRIPT
Supplemental Figure S1. Coleorhiza anatomy.
(A, B) Longitudinal median section of dry barley embryos from D (A) and AR (B)
grains showing coleorhiza enclosing the largest embryonic root. D and AR
embryos were indistinguishable. (C, D) Longitudinal median sections of barley
embryos 24 h after start of hydration from D (C) and AR (D) grains. (E, F) Higher
magnification of longitudinal sections of coleorhiza cells in dry barley embryos
from D (E) and AR (F) grains showing no difference in morphology or structure.
(G, H) Higher magnification of longitudinal sections of coleorhiza cells in barley
embryos after 24 h hydration from D (G) and AR (H) grains, showing some radial
expansion in D coleorhiza but more expansion as well as considerable elongation
and vacuolation in AR coleorhiza cells. (I, J) Transverse sections of coleorhiza and
enclosed roots in AR barley embryos before (I) and after (J) 24 h hydration
showing increased separation of coleorhiza cells from epidermis to root surface.
After hydration root cells were densely cytoplasmic but coleorhiza cells were
highly vacuolate. col = coleorhiza. (K, L) Longitudinal sections of cells at the tip
of the coleorhiza in AR barley seeds before (K) and after (L) 24 h hydration
showing near-isodiametric cell shape and isodiametric expansion of cells and
intercellular spaces.
Methods
(A-H, K, L) LR White sections were stained with PAS and counterstained with
0.5% fast green FCF in 95% ethanol, followed by 0.05% toluidine blue in
1% borax pH 4.4.
(I, J) Grains were fixed in 3% glutaraldehyde in 25 mM NaPO4 buffer, pH 6.8 for 2
hours at room temperature, then processed as above. Sections were stained with
0.1% toluidine blue in 1% borax, pH 4.4.
Supplemental Figure S1