sable’s scope who we are founded by john r.b. lighton, ph.d. 25 years of scientific discovery...
TRANSCRIPT
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SABLE’S SCOPEWho We Are
• Founded by John R.B. Lighton, Ph.D.• 25 years of scientific discovery• Tools for understanding – with a respirometry focus• Scientists and engineers still active in research• Growing at 25% for last 4 years• Growing at over 45% in 2011• Fueled by Promethion
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SABLE’S GLOBAL INFLUENCE
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Sable’s Promethion InspirationCUSTOMER REQUESTS:
Turnkey Solution
Fully Integrated
Live-in Cages with Bedding
Fully Traceable Data
Running Wheel
Expertise
High Throughput
Scalable
Accurate
User Friendly
No air tight seal
Good Customer Service
Customizable Analysis
No Chemical Desiccants
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MARKET RESEARCH: Competition
• Black box calculations• No raw data storage or data traceability• Customer service• Difficult to use• Very complex to set up
The Good
• Widely used and cited• Integrated system• High Throughput
The Bad
• Small, not live-in cages• No bedding in cages• Very low flow rates (IACUC)• Accuracy concerns• No integrated running wheels
The Ugly
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METABOLIC SCREENING
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EXISTING CUSTOMERS
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THE CAGE…
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FOOD INTAKE
Food Intake Monitor
Features
• 3mg resolution• Crumb tray reduces caching• Real-time measurement
• Universal Load Cell• Mouse or Rat hopper
Advantages
• Increased accuracy, versatility• Fine granularity gives insight into behavioral patterns
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FOOD INTAKERaw Feeding Data
• Stores hopper mass vs. time• Recorded to disc at 1Hz
One Feeding Episode
• Analyze behavior• Determine individual feeding episode intake rates
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FOOD INTAKESummarized Feeding Data
• Identify trends and outliers• Can zoom into any event• Automated uptake analysis• Automated meal analysis
One Feeding Episode
• Analyze behavior• Unprecedented detail available – but only if required
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FOOD INTAKEProcessed Feeding Data
• Outputs cumulative intake vs. time• Shows rate of intake, structure• Can output binned data• Integrates with other cage data• Fully traceable
Food Selection
• Determine food preference• Control access to either/both foods• Integrates with other cage data
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FOOD INTAKE ANALYSIS
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FOOD ACCESS CONTROL
Access Control Module
Features
• Computer controlled access to food• Available for Mouse or Rat food hoppers• Intelligent Obstruction Detection
• Access Control Door• Connects to mass sensor
Advantages
• Reduced technician interaction • Customizable assays (paired/yoked)
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FOOD ACCESS CONTROLUser Friendly Setup
• Select from 8 preset assays• Customize time and duration• Individual cage setup
Select Access Control Type
• Add to your custom assay• Unlimited number of variations
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WATER INTAKE
Water Intake Monitor
Features
• 3 µL resolution• Real-time measurement• Stainless steel case (not shown) reduces animal interaction• Custom sipper tube reduces leakage
• Universal Load Cell• Mouse or Rat dispenser models
Advantages
• Increased accuracy• Increased granularity gives insight into behavioral patterns• Use existing sterilizing systems
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WATER INTAKERaw Drinking Data
• Stores bottle mass vs. time• Recorded to disc at 1Hz
Processed Drinking Data
• Analyze behavior• Mathematical drift elimination
• Determine individual episode intake rates• EthoScan Compatible
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BODY MASS
Body Mass Monitor
Features
• 3mg resolution• Real-time measurement
• Universal load cell• Mouse or Rat models available
Advantages
• Highly accurate• Provides in-cage enrichment• Reduced technician interaction
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BODY MASSRaw Body Mass Data
• Stores bracket mass vs. time• Recorded to disc at 1Hz• Is used about every 15 minutes
Zoomed In
• Track body mass over time
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BODY MASSProcessed Body Mass Data
• Stores most recent body mass for each timestamp• Synchronized with other data
• Diurnal cycle clearly visible
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VOLUNTARY EXERCISE
Wheel-M or Wheel-R
Features
• Real-time measurement• Stainless steel construction• Entirely in cage
• Mouse or Rat Wheel• Magnetic Reed Switch• Optional: Wheel Stop
Advantages
• Increased granularity gives insight into behavioral patterns• Correlates with metabolic data
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VOLUNTARY EXERCISERaw Running Wheel Data
• Stores RPS vs. time• Recorded to disc at 1Hz
Processed Running Wheel Data
• Shows cumulative distance run• Can be binned and synchronized with metabolic data
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TOTAL ACTIVITY
BXYZ-M or BXYZ-R
• XY and Z IR arrays• Mounting bracket
Features
• 0.25cm calculated centroid• Real-time measurement• Intelligent obstruction detection• Rearing captured with Z axis
Advantages
• Highly accurate• Not affected by static obstructions• Correlated with metabolic data
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TOTAL ACTIVITYActivity Analysis
• Total Activity• Total Distance Traveled• Rearing• Activity associated with in-cage devices• In cage position vs. time• Customizable assays• Position histograms
Activity Comparison
• WT vs. ob/ob• Interaction with FH-MB• Time spent time spent near food hopper
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CALORIMETRY
Flow Generators & Gas Analyzers
• Automated calibration • Sequential or continuous monitoring• Can group for 4, 8,16, 24, 32 etc. cages• Expandable and modular• Water vapor dilution correction• Integrated O2, CO2, WVP analyzers
• Pull-mode (negative pressure)
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DATA ANALYSIS Data Synchronization
• Overlay metabolic data with other continuously monitored parameters
Further Analysis
• All data are perfectly synchronized and can be exported to other programs if required (open formats)
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DATA ANALYSIS Further Data Synchronization
•Body mass and food intake
Advantages
• Synchronized data yields traceability• Real time measurement improves visibility• Both enable behavioral monitoring
•Body mass and water intake
•Body mass, food intake, water intake, and wheel revolutions
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DATA ANALYSIS Multiplexed vs. Continuous
• Continuous measurements give unprecedented detail• Multiplexed measurements are adequate for many research topics• You have the choice
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TOTAL ACTIVITYActivity Analysis (II)
• Activity within user-defined areas of any shape• Regions of interest interactively defined on customizable background• Correlate with other data streams as needed
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TOTAL ACTIVITYActivity Analysis (III)
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TOTAL ACTIVITYActivity Analysis (IV)
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DATA ANALYSIS
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DATA ANALYSIS
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DATA ANALYSIS
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Multiplexing errors- more information
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Error from multiplexing
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Over and underestimates
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DATA ANALYSISRaw Data Synchronization - Zoomed
• Body mass (blk), food intake (red), wheel (yel), and water intake (blu)
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ETHOSCANBehavioral Monitoring
• All cage devices continuously monitored (“focal animal”)• Interactions sequentially processed in data analysis• Output is timestamp, position, duration, amount and activity
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ETHOSCAN
Behavioral Transition Matrix
• Statistical analysis of interaction with in-cage devices• Shows probability of behaviors in the top row happening immediately after the behavior in the leftmost column
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ETHOSCANTime Budgets
• Shows percentage of time spent exhibiting a specific behavior• Customizable to your cage setup and behaviors you wish to monitor
Ambulatory Budgets
• Two wild type mice –with and without access to a wheel• Note the significant difference in distance travelled
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ROOM CALORIMETRY
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ROOM CALORIMETRY• Time-consuming to set up and test• Just one span per run… But analyzers drift• Propane burn validations may require back-calculation of
span gases (black box!)• Cannot adequately account for water vapor dilution,
especially at low WVP• System integration difficult• Many individual instruments = many failure points and
many companies to deal with• Little or no expandability or modularity
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ROOM CALORIMETRY
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ROOM CALORIMETRY• Raw data not preserved, so - Not auditable• Comparisons between systems problematic• Little emphasis on traceability (pre-Jon Moon)• But – traceability alone will not help• Also need transparency!• Custom “black box” software is proprietary• Software is a priori optimized for
distinguishing between two activities
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ROOM CALORIMETRY• Highly stable O2, CO2 analyzers required
• Low noise (for response correction)• WV dilution is a problem…• Chemicals are impractical…• Typically, chilled condensers are used• But these only “dry” the air to ~650 Pa WVP• At dewpoints < 1 C, they have no effect at all• Plus, FiCO2 changes…
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ROOM CALORIMETRY
FiCO2 changes…
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ROOM CALORIMETRY +
• Minimize O2 drift (SSI O2 analyzers)
• Then, eliminate O2 drift by repeated, interleaved FiO2 measurement (PP)
• For CO2, use interleaved SSI analyzers (PP)
• For eliminating WV dilution, don’t scrub/condense – calculate from WVP, BP! (PP)
• Record all raw data in complete detail
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ROOM CALORIMETRY +• Use computational processing to reassemble
the dry O2 and CO2 data from the interleaved O2, CO2, WVP data, plus BP (PP)
• Apply rigorously neutral smoothing and response correction to data
• All using a traceable, auditable, shareable script
• Because raw data are stored, any kind of response correction you wish can be applied!
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PROMETHION
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PROMETHION
• Analyzers integrated (< time-lags)• All analyzers temperature-controlled• Calibration automated • Analog signal transfer eliminated• System is indefinitely expandable to future
sensors, e.g. bicycle W, treadmill speed/elevation, IR activity, accelerometry, body weight, heart rate, body mass, food intake, etc. etc.
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PROMETHION (room)
• No complex pressure etc. regulators required• Normocapnic, not steno/hypercapnic• High resolution analyzers plus background
baselining = higher flow rate (> 80 L/min)• Leak problems minimized• Single manufacturer = easy, single-point
support + FUD-less, quick setup!• Use for rooms, hoods or masks
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PROMETHION (room)
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PROMETHION• Push-button easy!• Raw data stored at 1 Hz• Any analytical protocol
can be applied• Drill down from processed
data to raw data• Users can share analytical
scripts
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PROMETHION• Additional sensors are in
development• All sensors are sampled at
1 Hz in perfect synchrony with gas exchange data
• Opens the door to many new questions
• Need a feature? Ask!
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PROMETHION
New Horizons
• Data Rate Changes from a trickle to a fire hose• Use as much as you need• Fully modular – add features as you need them• Automates analysis without losing flexibility and traceability• Sable Systems is committed to your success!
METABOLIC SCREENING
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Questions?
THANK YOU!