Peripheral Smear Sarmishtha Ghosh Physiology. CBC TOTAL RBC COUNT TOTAL WBC COUNT PERIPHERAL SMEAR –SHAPE, SIZE & COLOR OF RBCs –DIFFERENTIAL WBC COUNT.

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  • Peripheral SmearSarmishtha GhoshPhysiology

  • CBCTOTAL RBC COUNTTOTAL WBC COUNTPERIPHERAL SMEARSHAPE, SIZE & COLOR OF RBCsDIFFERENTIAL WBC COUNTPLATELET COUNTHEMOGLOBIN CONCENTRATIONBLOOD INDICES MCH, MCV, MCHC

  • 1423

  • Normal RBC distribution

  • HEAD- TOO THICK, SMALL & HYPOCHROMICTAIL- TOO THIN, MACROCYTIC & FLAT

  • SHAPE

  • HEMOGLOBIN CONTENT & DISTRIBUTION

  • WHITE BLOOD CELLS- LEUCOCYTES

  • NEUTROPHIL

  • EOSINOPHIL

  • BASOPHIL

  • LYMPHOCYTE

  • MONOCYTE

  • Hemocytometer

  • Blood IndicesThe relationships between the hematocrit, the hemoglobin level, and the RBC are converted to red blood cell indices through mathematical formulas.

    These formulas were worked out and first applied to the classification of anemias by Maxwell Wintrobe in 1934.

    The indices include these measurements: mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC); and red cell distribution width (RDW). They are usually calculated by an automated instrument as part of a complete blood count (CBC).

  • Mean corpuscular volume (MCV)

    MCV is the index most often used. It measures the average volume of a red blood cell by dividing the hematocrit by the red blood cell count.

    The MCV categorizes red blood cells by size.

    Cells of normal size are called normocytic, smaller cells are microcytic, and larger cells are macrocytic. Following formula is used to calculate MCV

  • Mean corpuscular hemoglobin concentration (MCHC)

    measures the average concentration of hemoglobin in a red blood cell. This index is calculated by dividing the hemoglobin by the hematocrit. The MCHC categorizes red blood cells according to their concentration of hemoglobin.

    Cells with a normal concentration of hemoglobin are called normochromic;cells with a lower than normal concentration are called hypochromic.

    Because there is a physical limit to the amount of hemoglobin that can fit in a cell, there is no hyperchromic category.

    Anemias are categorized as hypochromic or normochromic according to the MCHC index.

  • Mean corpuscular hemoglobin (MCH)

    The average weight of hemoglobin in a red blood cell is measured by the MCH. Anemias are categorized as hypochromic or normochromic or hyperchromic according to MCH index. MCH is most useful in diagnosis of severely anemic patients. Among those with lesser degrees of anemia, it is less useful because the measurement has a potential for a relatively wide range of error. MCH (in pg) = hemoglobin (in g/dL)x10 RBC count (in millions/mm3)

  • Normal results for red blood cell indices are as follows:MCV 82 to 98 fl (femtoliters) MCHC 31 to 37 g/dl (or %)MCH 26 to 34 pg (picograms)

  • LAB EXERCISESReferences cellbio.utmb.edu/microanatomy/Blood.htm Constructed by:Dr. Sarmishtha GhoshMBBS2012

  • IDENTIFY WITH JUSTIFICATION : a,b,c,d1

  • abcdef2

  • 31) What is the advantage of the biconcave shape of RBCs?2) Why is the absence of a nucleus an advantage?R- Red Blood CellN- NeutrophilE- Eosinophil

  • 41) What is the function of N & E respectively?2) The eosinophil count of a patient is 7%. What might be the probable indications?R- Red Blood CellN- NeutrophilE- Eosinophil

  • 5Identify X.What is it composed of?What is its function in the body?X

  • 6The photograph shows a MonocyteWhat made you identify it to be a Monocyte?Where would you expect monocytes to be in an infection?What is their function?

  • 7These cells in the are 25-33% of white blood cells.What is it and what is its function?

  • 8Identify Cell # 1

    Identify Cell # 2

    Identify Cell # 3

  • 9This is a Lymphocyte.State the Identifying featuresIs this a T lymphocyte or a B- lymphocyte

  • Comment on RBCs in the three slides A,B and C.10ABC

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