optical clearing of human skin for the enhancement of optical imaging of proximal interphalangeal...
TRANSCRIPT
OPTICAL CLEARING OF HUMAN SKIN FOR THE ENHANCEMENT OF OPTICAL IMAGING OF PROXIMAL INTERPHALANGEAL JOINTS
Ekaterina A. Kolesnikova1, Aleksandr S.Kolesnikov1, Urszula Zabarylo2,
Olaf Minet2, Elina A. Genina1, Alexey N. Bashkatov1,
Valery V. Tuchin1,3,4
1Saratov State University, 2Medizinische Physik und Optische Diagnostik,
Charité – Universitätsmedizin Berlin, 3Institute of Precision Mechanics and
Control RAS, 4Optoelectronics and Measurement Techniques Laboratory
e-mail: [email protected]
Saratov State UniversityDepartment of Optics
and Biophotonics
Saratov Fall Meeting 2013
Motivation
The transillumination optical imaging is a prospective technique for the diagnosis of rheumatoid arthritis in humans.
But the relatively low contrast and poor spatial resolution of the result images limits the usage of the technique for the finding of differences between healthy and pathology joints.
Goal of the study is to enhance the technique by reduction of skin light scattering at using of optical clearing method
Saratov State UniversityDepartment of Optics
and Biophotonics
Saratov Fall Meeting 2013
Materials and methods
Experimental setup
Fig. 1. The exterior of the experiment (left image) and the transillumination system with 3 laser diodes (right image).
Saratov State UniversityDepartment of Optics
and Biophotonics
Saratov Fall Meeting 2013
illuminating LEDs(670, 820, and 904 nm)
monochromatic digital CCD camera of an 8 bit dynamic range and variable exposure time
finger
The beam of the laser diodes was focused upon a spot diameter of ≤1 mm on the finger surface
Using laser diodes of a fixed power, the exposure time depends on the diameter of the finger and the manifestation of rheumatoid arthritis
Materials and methodsTested specimens
The experiments were carried out in vivo with human skin. Female (25 years) participated in the experiments. Proximal interphalangeal (PIP) joints of index finger (1.6 cm) and middle finger (1.8 cm) were investigated The scattered light images obtained by transillumination were taken from the PIP joints of index finger and middle finger to study the possibility of optical clearing application in optical diagnosis of rheumatoid arthritis
Optical clearing agents
Dehydrated glycerol and hand cream with urea (5%) were used as OCAs.
Refractive indices of glycerol are: 1.469 at 670 nm 1.466 at 820 nm 1.465 at 904 nm
Saratov State UniversityDepartment of Optics
and Biophotonics
Saratov Fall Meeting 2013
Materials and methodsContrast calculationContrast was calculated in region of interest (ROI) near the middle of the finger joint (fig.2) with special software designed in LabView programming environment
Fig. 2. An image of ROI for the contrast calculation (blue rectangle)
Saratov State UniversityDepartment of Optics
and Biophotonics
Saratov Fall Meeting 2013
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Fig. 3. An image of finger joint under the action of glycerol in a transmission mode: left image – before agent application, right image – 60 min after agent application.
Wavelength is 670 nm.
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Fig. 4. An image of finger joint under the action of glycerol in a transmission mode: left image – before agent application, right image – 60 min after agent application.
Wavelength is 820 nm.
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Fig. 5. An image of finger joint under the action of glycerol in a transmission mode: left image – before agent application, right image – 60 min after agent application.
Wavelength is 904 nm.
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Fig. 6. An image of finger joint under the action of hand cream in a transmission mode: left image – before agent application, right image – 60 min after agent application.
Wavelength is 670 nm.
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Fig. 7. An image of finger joint under the action of hand cream in a transmission mode: left image – before agent application, right image – 60 min after agent application.
Wavelength is 820 nm.
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Fig. 8. An image of finger joint under the action of hand cream in a transmission mode: left image – before agent application, right image – 60 min after agent application.
Wavelength is 904 nm.
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Table 1. Contrast in a ROI near the middle of the joint for the case of glycerol topical application
Time, min 670 nm 820 nm 904 nm
0 0.348 0.397 0.409
5 0.324 0.449 0.495
15 0.304 0.446 0.426
30 0.389 0.528 0.544
60 0.367 0.442 0.535
ResultsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Table 2. Contrast in a ROI near the middle of the joint for the case of hand cream with 5% urea application
Time, min 670 nm 820 nm 904 nm
0 0.505 0.465 0.530
5 0.518 0.503 0.542
15 0.505 0.525 0.596
30 0.412 0.439 0.490
60 0.471 0.414 0.458
SummarySaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
Thus, our results shows that glycerol and hand cream with
5% urea allow obtaining of more distinct image of finger
joint.
In that way we may conclude that investigated OCAs at
topical applications to skin can be effectively used as
enhancer for the technique for the finding of differences
between healthy and pathology joints in transillumination
mode.
AcknowledgementsSaratov State University
Department of Optics and Biophotonics
Saratov Fall Meeting 2013
This research was supported by:
Grant of President of RF NSH-1177.2012.2
FiDiPro, TEKES Program (40111/11), Finland
RF State contracts № 14.512.11.0022 and 14.B37.21.0728
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