list of peka biochapter 3 – movement of substances across the plasma membrane

7/27/2019 list of peka bioChapter 3 Movement of Substances across the Plasma Membrane

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Chapter 3 Movement of Substances across the PlasmaMembrane

List of PEKA experiments:

1. The movement of substances across a semi-permeablemembrane

2. To demonstrate the process of osmosis using simple osmometer3. Conducting a role-play to show the movement of substances

across the plasma membrane4. Studying the effects of hypotonic, hypertonic and isotonic

solutions on animal cells5. To study the effects of hypotonic, hypertonic and isotonic

solutions on plant cells (cell sap of onion cells)6. Determining the concentration of an external solution which is

isotonic to the cell sap of a plant


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Number Activity 3.1 (Experiment)Pg. 20Title The movement of substances across a semi-

permeable membraneAim /Objective of

the Study

To study the movement of substances across theVisking tubing

ProblemStatement

What factor influences the diffusion of substancesthrough a semi-permeable membrane?

Hypothesis The diffusion of molecules through a semi-permeable membrane is based on the size of themolecule

Variables Manipulated variable: Size of the solute moleculesin the Visking tubing

Responding variable: Colour of the solution inVisking tubing

Fixed variable: Time, volume of solutions andsurrounding temperature

Material Benedicts solution 1% starch suspension Iodine solution 30% glucose solution Distilled water

Apparatus Visking tubing Cotton thread Test tubes BeakersWater bath (Bunsen burner, tripod stand & wire

gauze)Techniqueused

Test for the presence of starch and glucose withiodine and Benedicts solution respectively

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Number Activity 3.2 (Experiment)Pg. 23Title Studying osmosis using an osmometer

Aim /Objective ofthe Study

To demonstrate the process of osmosis using simpleosmometer

Problem What substances can diffuse through a semi-


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Statement permeable membrane?Hypothesis Osmosis takes place when water moves from a

region of low solute concentration to a region ofhigh solute soncentration across a semi-permeablemembrane

Variables Manipulated variable: Concentration of solution Responding variable: Increase in the level ofsucrose solution / The level of sucrose

solution in the capillary tube Fixed variable: Concentration of sucrose solution,

surrounding temperatureMaterial 30% sucrose solution

Distilled waterApparatus 25 cm capillary tube

Visking tubing Retort stand and clamp Cotton thread Ruler Marker penA pair of scissors 250 ml beaker Syringe Stopwatch

Techniqueused

Measure and record the increase in the height ofsucrose solution with a ruler.

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Number Activity 3.3 (Simulation)Pg. 26Title Conducting a role-play to show the movement of

substances across the plasma membraneAim /Objective of

the Study

To study the movement of soluble substancesacross the plasma membrane through the

process of passive transport. To study the movement of water molecules across

the plasma membrane by osmosis To study the movement of substances across the

plasma across the plasma membrane throughthe process of active transport.

Material Strings


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CardboardA pair of scissors Marker pen

Apparatus -Techniqueused

Conduct an interesting and creative role-play tolearn the movement of substances across the

plasma membrane (phospholipid bilayer)================================================

Number Activity 3.4 (Observation)Pg. 27Title Studying the effects of hypotonic, hypertonic and

isotonic solutions on animal cellsAim /

Objective ofthe Study

To study the effects of hypotonic, hypertonic and

isotonic solutions on animal cells (red blood cells)

Material Fresh chicken blood 0.15 M sodium chloride solutions 0.50 M sodium chloride solutions Distilled water Filter paper

Apparatus Glass slides Cover slips Filter papers Light microscope Test tubes Beakers Dropper

Techniqueused

Observe and study the effects of hypotonic,hypertonic and isotonic solutions on animal cellsfrom the slides by using microscope

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Number Activity 3.5 (Observation)Pg. 28Title Studying the effects of hypotonic, hypertonic and

isotonic solutions on plant cellsAim / To study the effects of hypotonic, hypertonic and


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Objective ofthe Study

isotonic solutions on plant cells (cell sap of onioncells)

Material Onion bulb 0.5 M sucrose solutions 1.0 M sucrose solutions Distilled water

Apparatus Light microscope Glass slides Cover slips Razor blade / scalpelA pair of forceps Mounting needle Filter papers

Techniqueused

Observe and study the effects of hypotonic,hypertonic and isotonic solutions on plant cellsfrom the slides by using microscope.

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Number Activity 3.6 (Experiment)Pg. 30Title Determining the concentration of an external

solution which is isotonic to the cell sap of a plantAim /Objective ofthe Study

To determine the concentration of an externalsolution which is isotonic to the cell sap of a plant

ProblemStatement

What is the concentration of an external (sucrose)solution which is isotonic to the cell sap of plants?

Hypothesis The concentration of the solution which isisotonic to the cell sap of plant cells has noeffect on the mass, length and size of plantcells.

0.36 M sucrose solution is isotonic to the cell sapof plants

Variables Manipulated variable: Concentration of sucrosesolution Responding variable: Length of potato strips /

Mass of potato strips Fixed variable: Volume of sucrose solution used,

surrounding temperature and timeMaterial Fresh potato


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Distilled water 0.1 M sucrose solution 0.2 M sucrose solution 0.3 M sucrose solution 0.4 M sucrose solution 0.5 M sucrose solution0.6 M sucrose solutionApparatus Petri dishes

A pair of forceps Razor blade / Sharp scalpel Cork borer Ruler 50 ml beakers Electronic scale / electronic balance Tissue paper

Technique

used

Measure the length of potato strips with ruler Determine the percentage difference in length Mass of potato strips with an electronic balance Determine the percentage difference in mass

list of peka biochapter 3 – movement of substances across the plasma membrane

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