intracellular compartments er, golgi, endsomes, lysosomes and peroxisomes

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Intracellular Intracellular Compartments Compartments ER, Golgi, Endsomes, ER, Golgi, Endsomes, Lysosomes and Peroxisomes Lysosomes and Peroxisomes

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Page 1: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Intracellular Intracellular CompartmentsCompartments

ER, Golgi, Endsomes, ER, Golgi, Endsomes, Lysosomes and PeroxisomesLysosomes and Peroxisomes

Page 2: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Endomembrane SystemEndomembrane System

Page 3: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Endoplasmic ReticulumEndoplasmic Reticulum

Of what does the endoplasmic Of what does the endoplasmic reticulum consist?reticulum consist?

What distinguishes the rough ER What distinguishes the rough ER from the smooth ER?from the smooth ER?

Page 4: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Endoplasmic ReticulumEndoplasmic Reticulum

How do ER membranes compare with How do ER membranes compare with the plasma membrane?the plasma membrane?

Page 5: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Endoplasmic ReticulumEndoplasmic Reticulum

What cellular What cellular processes take processes take place in the place in the smooth ER?smooth ER?

What is aryl What is aryl hydrocarbon hydrocarbon hydroxylase?hydroxylase?

Page 6: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Endoplasmic ReticulumEndoplasmic Reticulum

What is the fate of proteins made on What is the fate of proteins made on the RER?the RER?

What happens to proteins before What happens to proteins before they leave the ER?they leave the ER?

Page 7: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Endoplasmic ReticulumEndoplasmic Reticulum

What else takes place in the ER?What else takes place in the ER?

How do newly made phospholipids How do newly made phospholipids get to other cell organelles?get to other cell organelles?

Page 8: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Golgi ComplexGolgi Complex

Of what does the Of what does the Golgi consist?Golgi consist?

Page 9: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Golgi ComplexGolgi Complex

How do the Golgi membranes How do the Golgi membranes compare with those of the ER?compare with those of the ER?

What enzymes are associated with What enzymes are associated with this organelle?this organelle?

How are the different vesicles How are the different vesicles associated with Golgi distinguished?associated with Golgi distinguished?

Page 10: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Golgi ComplexGolgi Complex

What two models describe the flow of What two models describe the flow of lipids and proteins through the Golgi lipids and proteins through the Golgi complex?complex?

How do anterograde transport differ How do anterograde transport differ from retrograde transport?from retrograde transport?

Page 11: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Golgi ComplexGolgi Complex

What is glycosylation?What is glycosylation?

What are the two forms of What are the two forms of glycosylation?glycosylation?

Page 12: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Golgi ComplexGolgi Complex

How does How does glycosylation take glycosylation take place?place? begins in ERbegins in ER continues in Golgicontinues in Golgi

Page 13: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Protein SortingProtein Sorting

How are proteins sorted as they move How are proteins sorted as they move through ER and Golgi?through ER and Golgi?

Page 14: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Protein SortingProtein Sorting

How are lysosomal How are lysosomal enzymes targeted?enzymes targeted?

How do we know How do we know

this proposed this proposed pathway is valid?pathway is valid?

Page 15: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Protein SortingProtein Sorting

What evidence supports secretory pathway?What evidence supports secretory pathway?

Page 16: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Protein SortingProtein Sorting

Page 17: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Protein SortingProtein Sorting

What is the difference between What is the difference between constitutive and regulated secretion?constitutive and regulated secretion?

Page 18: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Exocytosis and EndocytosisExocytosis and Endocytosis

What is involved in What is involved in exocytosis?exocytosis?

What appears to What appears to trigger this trigger this process?process?

Page 19: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Exocytosis and EndocytosisExocytosis and Endocytosis

What is involved in What is involved in endocytosis?endocytosis?

What happens to What happens to endocytic vesicles?endocytic vesicles?

Page 20: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Exocytosis and EndocytosisExocytosis and Endocytosis

What are the three forms of What are the three forms of endocytosis?endocytosis?

Page 21: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

PhagocytosisPhagocytosis

What kinds of cells What kinds of cells carry out carry out phagocytosis?phagocytosis?

Page 22: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Receptor-Mediated Receptor-Mediated EndocytosisEndocytosis

How is this process different from phagocytosis?How is this process different from phagocytosis?

Page 23: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes
Page 24: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

Clathrin-Independent Clathrin-Independent EndocytosisEndocytosis

What function does this process What function does this process appear to serve?appear to serve?

Page 25: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

LysosomesLysosomes

What are some of the characteristics of What are some of the characteristics of these organelles?these organelles?

Page 26: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

LysosomesLysosomes

How are these How are these organelles formed?organelles formed?

What functions do What functions do they serve?they serve?

Page 27: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

LysosomesLysosomes

What is the difference between an What is the difference between an early endosome, a late endosome early endosome, a late endosome and a lysosome?and a lysosome?

Do all late endosomes become Do all late endosomes become lysosomes?lysosomes?

Page 28: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

LysosomesLysosomes

In what way do lysosomes play key In what way do lysosomes play key roles in both cell defense and roles in both cell defense and nutrition?nutrition?

What are residual bodies?What are residual bodies?

What is the difference between What is the difference between autophagy and autolysis?autophagy and autolysis?

Page 29: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

LysosomesLysosomes

Under what conditions are lysosomal Under what conditions are lysosomal enzymes released outside of the cell?enzymes released outside of the cell?

What causes lysosomal storage What causes lysosomal storage diseases?diseases?

Page 30: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

PeroxisomesPeroxisomes

What technique enabled DeDuve to What technique enabled DeDuve to successfully isolate these organelles?successfully isolate these organelles?

Page 31: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

PeroxisomesPeroxisomes

What are some of the distinguishing What are some of the distinguishing characteristics of this organelle?characteristics of this organelle?

How are they identified How are they identified cytochemically?cytochemically?

What functions are associated with What functions are associated with peroxisomes?peroxisomes?

Page 32: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

PeroxisomesPeroxisomes

Why does hydrogen peroxide Why does hydrogen peroxide accumulate in these organelles?accumulate in these organelles?

In what way does this organelles In what way does this organelles contribute to fatty acid oxidation?contribute to fatty acid oxidation?

From where do these organelles From where do these organelles come?come?

Page 33: Intracellular Compartments ER, Golgi, Endsomes, Lysosomes and Peroxisomes

PeroxisomesPeroxisomes

What types of unusual peroxisomes What types of unusual peroxisomes are found in plants?are found in plants?