infection control logbook 1ƒراسة... · · 2012-06-06safe centrifugation ... glassware deal...

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Infection control

Logbook 1 Basic Microbiology and Immunology

Production Design:

360 Extensive [email protected]

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وب ردتايسلس ب ردس و فشارس و تسيفشتسملا

فشس وب ردا فشس وب ردا

يفشتسس فابس وب ردا (ميفس ل لااس و فشار)

يفشتسس فابس وب ردا (ميفس ل لااس و فشار)

ىايشسمياشس وب ردا ىايشسمياشس وب ردا

فشس وب ردا فشس وب ردا

يفشتسس فابس وب ردا يفشتسس فابس وب ردا (ميفس ل لااس و فشار)

(ميفس ل لااس و فشار)

ىايشسمياشس وب ردا ىايشسمياشس وب ردا

متالايسمنعتةسصخس وب فشت

مفشس

ياشامس وبتراس

وينف نسسس

شششسيبىت س د لوةس وبلنةسس

شششس و لتدفنس

شششس وبابفقس

ويبافس لوا بللتسس

و نع سسس

ياشامس وايفقسماود اوةس وبعباةسس

ةفشخسمنعتة

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Introduction …………………..………….………….………… 7

Safe Laboratory Practices ………………………………….. 9

Practical Course of Bacteriology…………………………… 19

Practical Course of Virology & Immunology……………… 31

Rotation Proof…………………………………………………. 37

CONTENTS...

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The personal progress “logbook” is an important record of the candidate's

training and scientific activities in the field of Infection Control. Any

trainee should fulfill the minimum requirements of basic training and sci-

entific activities in the module of Medical Microbiology and Immunology.

All activities per topic should be covered. The council has the right to

mark certain activities in the logbook as obligatory and given priority over

other activities.

Each activity covered by the trainee should be registered, signed and

scored in this logbook by the trainer in charge.

Other activities covered but not stated in this logbook can be filed and

later evaluated and scored by authorized council member.

A minimum of 100 points are required to sit for the first part exam. The

scoring will be as follows

Each trainee is required to present his/her personal logbook at the end of

the basic training course. The completed logbook will be reviewed and

assessed by the council members.

Head of the infection control scientific council

Professor Dr Osama Rasslan

INTRODUCTION

Subject Score

Safe laboratory practice 10

Bacteriology 60

Virology &Immunology 30

Total 100

9

Safe Laboratory Practices

Will be assessed on daily basis during

training times

10

Skill Date Supervisor

Sign. Score

1. Pipetting technique

Never mouth-pipette

Use safe measuring and dispensing

devices

Disposal of tips

2. Behaviors

Never eat , drink, store food or

apply cosmetics in working area

3. Use of aseptic techniques when

handling specimens and culture

4. Hand washing

5. Wearing of appropriate protective

clothing

Ensure correct decontamination and laundry

6. Wearing gloves, face shield (when

indicated)

No use of phone or doing clerical work with working gloves

7. Safe centrifugation

Minimize creation of aerosol


Both facing pages constitutes one table. The table tolerate scoring of six visits

11


Sign. Score

8. Avoid practice resulting in nee-

dle stick injury

No use of chipped or cracked glassware

Deal with breakage immediately

and safely

9. Work neatly and keep the bench

surface free of unnecessary materi-

als

10.Decontaminate working surfaces

at the end of each day work

11. Avoid spillage

Know what to do if spillage occurs

Reporting any spillage and any expo-sure to infectious material

12. Decontamination of specimens

and other infectious materials

13. Dispose of laboratory waste

safely

14. No overfill of discard containers

Use of appropriate disinfectants

Use of separate container for sharps

15. Not allowing unauthorized per-

sons to enter the lab


12


Sign. Score


Never mouth-pipette


devices

Disposal of tips

2. Behaviors





4. Hand washing


clothing



indicated)






13


Sign. Score


dle stick injury



and safely



als



11. Avoid spillage






safely







14


Sign. Score


Never mouth-pipette


devices

Disposal of tips

2. Behaviors





4. Hand washing


clothing



indicated)






15


Sign. Score


dle stick injury



and safely



als



11. Avoid spillage






safely







16


Sign. Score


Never mouth-pipette


devices

Disposal of tips

2. Behaviors





4. Hand washing


clothing



indicated)






17


Sign. Score


dle stick injury



and safely



als



11. Avoid spillage






safely







19

Practical Course of

Bacteriology

20

Module activities and requested skills

Skills Page number

Microscopy 21

Preparation and fixation of smears 21

Staining 22

Culture media and preservation 22

Inoculation of culture media 23

Tests for bacterial identification 23-24

Collection of specimens for bacteriological

examination

24-25

Examination of LRT specimens 26

Examination of fecal specimens 27

Examination of urine specimens 27

Examination of pus 28

Blood culture 28

Antimicrobial susceptibility testing 29

Sterilization and disinfection 29

Examination of water 30

21


Sign.

Score

1. L/M microscope

“Stained preparations".

2. Fluorescent microscope

“Demonstration”

3. Dark field microscope

“Demonstration”

Practical Course of Bacteriology

Microscopy

Preparation and Fixation of Smear


Sign.

Score

1. Labeling of slides

2. How to make smears: from

Cultures

Swabs

Sputum

Urine

CSF

Fecal specimen

3. Fixation of smears

22

Staining


Sign.

Score

Preparation of stained smears:

Simple stain

- Methylene blue

Differential stain

- Gram stain (+ve & -ve)

- Acid Fast stain for T.B

Z.N. stain “hot method”

Kinyoun method “cold method”

Culture Media and Preservation of Stock Cultures

Skill Date Supervisor Sign.

Score

1. Preparation of different culture media:

-Nutrient agar - Blood agar

-MacConkey' s agar - Chocolate agar

-Loffler's serum - Lowenstein Jensen

- Blood tellurite - Robertson cooked

meat broth

- Bile esculin - Mannitol salt agar

- TCBS - DCA

- CLED

2. Preservation of stock culture:

- Soft agar

-Long preservation

-Lyophilization

3. Preparation of media for biochemi-

cal reactions

23

Inoculation of Culture Media


Sign.

Score

1. Aseptic techniques

2. Inoculation of specimens on culture plates

3. Inoculation of slopes

4. Inoculation of deep agar medium

5. Inoculation of fluid media

6. Labeling of inoculated media

7. Incubation of inoculated media: tem-perature, humidity, different gaseous

atmosphere.

8. Anaerobic culturing :

- Anaerobic jar & Gas Pack System

Tests for Bacterial Identification


Sign.

Score

1. Catalase test

2. Coagulase test

3. Bile solubility test

4. Citrate utilization

5. DNase test

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Skill Date Supervi-

sor Sign.

Score

6. Indole test

7. Litmus milk test

8. Oxidase test

9. Urease test

10. Sugar fermentation tests

11. MIO, LIA, TSI tests

12. Bacterial identification by API system

Collection of Specimen for Bacteriological Examination


Sign.

Score

1- Pus :

Swabs

Syringe

2- Upper respiratory tract specimens:

Conjunctival swab

Throat swab

Nasopharyngeal swab

Anterior naris swab

Nasal wash

Tests for Bacterial Identification

25

Collection of Specimen for Bacteriological Examination


Sign.

Score

Lower respiratory tract specimens

Sputum

Induced sputum

Endotracheal-aspirate (ETA)

BAL

PSB

2. Urine specimen

MSU

From catheterized patients

Infants

3. High vaginal swab

Endo cervical swab

4. CSF by lumbar puncture

5. Blood for blood culture

6- Fecal specimens

Stool and diarrheal fluid

Rectal swabs

7- Environmental samples.

Surface samples

-Swabbing methods

-Rinsing method

Fluid samples: water and dialysate fluid

samples from hemodialysis units

26

Examination of LRT specimens


Sign.

Score

1. Specimen

Macroscopic appearance

2. Preparation of specimen

Liquefaction

Liquefaction & Decontamination

3. Microscopical examination

- Testing reliability of the specimen

Gram smear

Z.N. stain

Cold stain for AFB

4. Culture and quantitative culture

Blood agar

Chocolate agar

Media for isolation of tab

5. Identification and reporting.

27


Sign.

Score

1. Specimen (macroscopic appearance)

2. Enrichment and Culture

MacConkey' s agar

DCA, S & S

Hektoen agar media

TCBS

3. Identification, Confirmation (slide agglutination tests) and reporting.

Examination of Fecal Specimens

Examination of Urine


Sign.

Score

Specimen (Macroscopic appearance)

Dip stick test


Gram-stain

Wet preparation

3. Quantitative Culture

Semi quantitative: Dip Slide

4. Identification & reporting

28

Examination of Pus


Sign.

Score


2. Culture (Blood Agar & MacConkey' s agar media)

(aerobic & anaerobic)

3. Culture Identification & reporting.

Blood Culture


Sign.

Score

1. Inoculation of blood culture bottle

2. Examine microscopically

3. Subculture on:

Blood & Chocolate agar

MacConkey' s agar

4. Culture examination and reporting of significant growth

29

Antimicrobial susceptibility testing


Sign.

Score

Disc Diffusion

Min. inhibitory conc.(MIC)

Min. bactericidal conc.(MBC)

E-Test

Detection of:

MRSA

VRSA

VRE

ESBL

Other B lactamases producers

Sterilization and Disinfection


Sign.

Score

1. Use of autoclave

2. Test of autoclave efficiency

Chemical

Biological

3. Use of hot air oven

4. Sterilization by Seitz filter and other filters

30


Sign.

Score

1. Collection of water samples from

different sources

2. Bacteriological testing of water

Membrane filtration technique

Inoculation of culture bottle, tubes (Most probable number)

(Coliforms, Enterococci, Pseudomonas,

Staphylococci)

Colony count ( pour plate method)

3. Subculture on:

MacConkey' s agar

Eijkman's test

Bile Esculin hydrolysis test

4. Examination and reporting

Examination of Water

31

Practical Course of

Virology & Immunology

32

Module activities and requested skills

Skill Page number

Micropipette 33

Inverted microscope 33

Isolation of viruses from clinical specimens 33

Detection of specific viral antigen or anti-

bodies

34

Detection of viral nucleic acid 34

Agglutination tests 35

Antistreptolysin O test titration 35

ELISA 36

Tuberculin test 36

33

Practical Course of Virology & Immunology

Micropipettes


Sign.

Score

1. Different types and applications

2. How to use it


Sign.

Score

1. Preparation of specimens before

inoculation

2. Required equipments and reagents


Sign.

Score

1. Uses

2. Interpretation of results

Isolation of the Virus from Clinical Specimens

Inverted Microscope

34


Sign.

Score

IFT

NT

HIT

Detection of Specific Viral Antigens and/or Antibodies by

Serological Tests for the Diagnosis of Viral Infection

Detection of Viral Nucleic Acid in Clinical Specimens


Sign.

Score

Nucleic acid hybridization

Polymerase chain reaction (PCR)

35

Agglutination tests (slide & tube)

Widal test Brucella testing


Sign.

Score

1. Separation of patient serum

2. Serial dilution of patient serum (micropipette)

3. Addition of patient serum to antigen

4. Preparation of controls

5. Proper conditions for reactants

6. Interpretation of results

Antistreptolysin O titration


Sign.

Score



3. Addition of patient serum to RBCS

4. Addition of streptolysin O


6. Proper conditions for reactant

7. Interpretation of the results

36

Tuberculin test

ELISA


Sign.

Score

1. I.D. injection of PPD

2. Measure induration reaction



Sign.

Score



3. Addition of patient serum to wells of microtiter plates

4. Washing step

5. Addition of substrate

6. Addition of human anti γ globulin


8. Proper conditions for reactants


37

Rotation

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infection control logbook 1ƒراسة... · · 2012-06-06safe centrifugation ... glassware deal...

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