Group R14300 - Microfluidics

Peter DunningPaulina KlimkiewiczMatthew PartaczAndrew GreeleyThomas WossnerWunna KyawGroup R14300 Digital Microfluidics1Need for point of care medical testing devices where access to conventional tests is restrictedEx: Doctors Offices, Remote Areas, Battlefields

A solution must be portable and cheap

Motivationhttp://gdb.voanews.com/6C27D536-9955-4670-91C4-101E2D5A6322_w640_r1_s.jpg

Paulina2Lab-on-a-chip devices are capable of miniaturizing and automating biological protocols.

Devices suited for commercial use have yet to be developed.

Background

http://2.imimg.com/data2/GK/EX/MY-920622/micro-biological-testing-250x250.jpghttp://www.lionixbv.nl/technology/technology-microfluidics.html

Matt

3

Microfluidic PlatformsMajor microfluidic platforms organized by liquid actuation [1]Matt4Array of electrodes which use the electrowetting effect to manipulate droplets.

Digital MicroFluidic devices

Top view of flow on a ring structure [3]Cross-section view of Digital Microfluidic device. Dotted line indicates the shape of the meniscus before actuation. Modified from [2]

The electrowetting effect (in air) [3]Peter5Customer Requirements1.1Droplet ManipulationCapable of creating droplets1.2Droplet ManipulationCapable of splitting droplets1.3Droplet ManipulationCapable of moving droplets1.4Droplet Manipulationcapable of Merging droplets1.5Droplet ManipulationRepeatability of motion1.6Droplet ManipulationCreate a fast switching time1.6.1Droplet Manipulation Generate signal1.6.2Droplet Manipulation Control switches2.1MonitoringCheck location of droplet2.2MonitoringMeasure capacitance2.3MonitoringMeasure resistance3.1AutonomizationFollow a programmed sequence of instructions3.1.1AutonomizationVisual interface to create and control droplet pathways3.2AutonomizationMultiplexing3.3AutonomizationTest for ELISA (Enzyme Linked Immunosorbant Assay)Tom 1,2Wunna 3,4

6Customer Requirement Cont.4.1FabricationObtain benchmark droplet speed4.2FabricationLow cost5.1PackagingSmall5.2PackagingLight weight5.3PackagingDurable5.4PackagingReusable5.5PackagingPackage reagants with device5.6PackagingUser friendly5.7PackagingApplication based modularity5.8PackagingMinimize evaporation (reagant dependent)5.8.1Packaging Control humidity of system5.8.2Packaging Control temperature of device5.9PackagingVisually accessible5.10PackagingClean environmentWunna 3,4Andrew 57Outstanding VOCWe will be meeting with Dr. Schertzer in the near future to confirm these requirements.

Questions for AudienceAre our goals for this project clearly defined?Do you have some understanding of how Digital Microfluidic Devices work?

Andrew8[1] Mark, D., Haeberle, S., Roth, G., Von Stetten, F., and Zengerle, R., 2010, "Microfluidic Lab-on-a-Chip Platforms: Requirements, Characteristics and Applications," Chemical Society Reviews, 39(3), pp. 1153-1182.[2] Cho, S. K., Moon, H. J., and Kim, C. J., 2003, "Creating, Transporting, Cutting, and Merging Liquid Droplets by Electrowetting-Based Actuation for Digital Microfluidic Circuits," Journal of Microelectromechanical Systems, 12(1), pp. 70-80.[3] Fair, R., The Electrowetting Effect (in Air), February 1, http://microfluidics.ee.duke.edu/

References