microfluidics congress: usa

July 10-12, 2016, Philadelphia, PA

www.globalengage.co.uk/microfluidics-usa.html #GEMFC16

Microfluidics Congress: USA UTILIZING MICROFLUIDIC TECHNOLOGIES AS A TOOL FOR PROGRESSING MEDICAL

RESEARCH AND PATIENT CARE

Building on the success of our Microfluidics Congress 2015 in London, Global Engage is pleased to announce the Microfluidics Congress: USA,

which will be held on July 10-12, 2016 in Philadelphia, Pennsylvania at the Hilton Philadelphia City Avenue. The event will be co-located with the 2nd qPCR & Digital PCR Congress: USA. There will also be pre-meeting training courses. For more information on the training courses, please see the end of this document.

Microfluidics is a rapidly developing area of research, and scientists are continually discovering the wide range of possibilities the technology can provide. At the intersection of engineering, physics, chemistry, nanotechnology and biotechnology, microfluidics is revolutionizing the way patients are diagnosed, monitored and treated, and is unlocking the potential for reduced reagent consumption and thus, cost. Attracting experts working in all areas of microfluidics, the conference will examine the latest developments in the technologies and techniques being used for progressing medical research, as well as the challenges and future of microfluidics. Should you be an expert in developing microfluidics technologies, or a scientist using microfluidics to further medical research, the conference will be an excellent opportunity to learn, share, discuss and engage with the most current microfluidics research and technology. During the two-day conference, there will be networking breaks to promote interaction with your peers, expert led case study presentations, and a dynamic exhibition room filled with technology providers showcasing their technologies and solutions.

Confirmed Speakers Include:

Nicole Pamme Professor in Analytical Chemistry, University of Hull, UK

Richard Fair Lord-Chandran Professor of Engineering, Department of Electrical and Computer Engineering, Duke University, USA

Steven Soper Professor, Department of Biomedical Engineering and Department of Chemistry, University of North Carolina, Chapel Hill, USA

Conference Synopsis

Day One – Monday July 11th

Strategy and Technology in Microfluidics

Commercialization of microfluidic technology

Droplet microfluidics

Digital microfluidics

Centrifugal microfluidics

Organ-on-a-chip platforms

Dielectrophoresis

Paper-based systems

Optofluidics

Inertial microfluidics

Acoustofluidics

Gas microflows

Sensing technologies

Modelling and simulation

Novel fabrication techniques

3-D printing of microfluidic devices

Technology patent law

Day Two – Tuesday July 12th

Case Studies and Applications in Medical Research

Point-of-care diagnostics and disease monitoring

Isolating and analysis of circulating tumor cells (CTCs)

Single cell analysis

Synthetic biology

Lab-on-a-chip

DNA analysis

Biomaterials and tissue engineering

Biomarker analysis

Proteomics

Cell sorting

High throughput screening

Training Courses – Sunday July 10th 1. Microfluidics and Lab-on-a-Chip Technologies for commercial product

development: Strategies, Technologies, Markets and Applications 2. Basic Principles in Lab-on-a-Chip (LOC) Technologies for the Isolation of

Circulating Markers: Application in Liquid Biopsies

For more information please contact Steve Hambrook, Conference Director, Global Engage Ltd.

[email protected] +44 (0) 1865 849841

Confirmed Speakers

Richard Fair Lord-Chandran Professor of Engineering, Department of Electrical and Computer Engineering, Duke University, USA Charles Henry Professor, Department of Chemistry, Colorado State University, USA

Tony Jun Huang Professor and The Huck Distinguished Chair in Bioengineering Science and Mechanics, The Pennsylvania State University, USA Anne-Marie Gué Research Director, LAAS-CNRS, France Piotr Garstecki Professor, Group Leader, Institute of Physical Chemistry, Polish Academy of Sciences, Poland Eric Forssen Principle Scientist and Manager Drug Delivery Sciences, Allergan PLC, USA José Luis García-Cordero Assistant Professor, Bio-ARTS Lab, Cinvestav-IPN, Monterrey, Mexico Janine Nunes Associate Research Scholar and Lecturer, Department of Mechanical and Aerospace Engineering, Princeton University, USA Chair: Changchun Liu Research Assistant Professor, Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, USA

Chair: Noah Clay Director, Quattrone Nanofabrication Facility, Penn Singh Center for Nanotechnology, USA

Maryam Tabrizian Professor, Guggenheim Fellow in Biomedical sciences, McGill University, Canada Michael Shuler Samuel B. Eckert Professor of Engineering, Cornell University and CEO/President of Hesperos, Inc., Orlando, FL, USA Nicole Pamme Professor in Analytical Chemistry, University of Hull, UK Leslie Yeo Professor of Chemical Engineering & Australian Research Council Future Fellow, School of Engineering, RMIT University, Australia David Juncker Professor, Micro & Nano Bioengineering Lab, McGill University, Canada David Issadore Assistant Professor, Bioengineering, Electrical and Systems Engineering, University of Pennsylvania, USA Holger Becker Co-founder and CSO, microfluidic ChipShop GmbH, Germany Leanna M. Levine PhD, President, ALine, Inc., USA Chair: Collin Edington Postdoctoral Researcher, Massachusetts Institute of Technology, USA

Steven Soper Professor, Department of Biomedical Engineering and Department of Chemistry, University of North Carolina, Chapel Hill, USA Steve Wereley Professor of Mechanical Engineering, Birck Nanotechnology Center, Purdue University, USA Carolyn Ren Professor, Canada Research Chair in Droplet Microfluidics and Lab-on-a-Chip Technology, Department of Mechanical and Mechatronics Engineering, University of Waterloo, Canada Yoon-Kyoung Cho Assistant Professor in Biomedical Engineering, UNIST and Group Leader in Center for Soft and Living Matter, IBS, Korea Joseph Valentino PhD, Associate, Fish & Richardson P.C., USA Daniel Levner PhD, Chief Technology Officer, Emulate, Inc., USA Ian Papautsky Professor, Department of Electrical Engineering and Computing Systems, University of Cincinnati, USA Adela Ben-Yakar N. Doug Williams Memorial Centennial Endowed Faculty, Professor, The University of Texas at Austin, USA Chair: Tania Konry Assistant Professor, Department of Pharmaceutical Sciences, Northeastern University, USA

Microfluidics Congress: USA – July 11-12, 2016, Philadelphia, PA

The Microfluidics Congress: USA is co-located with the:

2nd qPCR and Digital PCR Congress USA Bringing together over 175 industry and academic experts working in areas such as molecular biology/diagnostics, gene expression, genomics, biomarkers, pathogen detection, GMO, mRNA, NGS, bioinformatics and data management, the congress will examine the latest developments, opportunities and applications of both dPCR and qPCR through case studies across diverse areas such as oncology, infectious diseases, vaccines,

prenatal diagnostics, clinical applications, microbiology, food microbiology, environmental testing and other novel applications. http://www.globalengage.co.uk/digital-and-qpcr.html

Microfluidics Congress: USA & 2nd qPCR and Digital PCR Congress USA Sponsors

Gold Sponsors

Sponsors

Sponsors from 2015

Microfluidics Congress: USA

8:00-8:50 Registration & Coffee Room: RENAISSANCE & GRAND SALON

8:50-9:00 Room: VERSAILLES Chair’s Opening Remarks: Noah Clay, Director, Quattrone Nanofabrication Facility, Penn Singh Center for Nanotechnology, USA

9:00-9:35 Keynote Address:

Constructing “Body‐on‐a‐chip” Devices for Drug Development Effective human surrogates, created from combining human tissue engineered constructs with microfabricated systems, could have a major impact on drug development, particularly in making better decisions on which drugs to take into human clinical trials. These systems are often called microphysiological systems. Our designs are guided by physiologically based pharmacokinetic models. We will describe approaches to use “pumpless”, low cost platforms to build such human surrogates for evaluation to toxicity and efficacy of drug candidates. These systems can incorporate functional measures, such as electrical activity and force generation, as well as metabolic responses. Confirmed: Michael Shuler, Samuel B. Eckert Professor of Engineering, Cornell University and CEO/President of Hesperos, Inc., Orlando, FL, USA

9:35-10:05 Organs-on-Chips: emulating human biology by engineering cellular microenvironments Organs-on-Chips are a novel microfluidic platform that may be used to emulate human biology in vitro, helping us understand how different diseases, medicines, chemicals and foods may affect human health. In this presentation, we review our system and its incorporation of tissue-tissue interfaces, biochemical cues, mechanical forces and physiological perfusion in an organ-specific context. Our experience from organs such as the lung, liver, intestine and kidney consistently shows that the engineered microenvironments present in our Organs-on-Chips lead human cells to assume in vivo function and effectively reproduce organ-level physiology and disease responses. Based on experimental data collected from this system, it is evident that Organs-on-Chips stand as a more predictive, human-relevant alternative to traditional in vitro and animal-based approaches. Confirmed: Daniel Levner, PhD, Chief Technology Officer, Emulate, Inc., USA

10:05-10:30 Let there be cartridge: Strategies and technologies for fully integrated microfluidic cartridge development Microfluidics has developed into a true enabling technology in any new product development in diagnostics and the life sciences. In recent years, one of the early promises, the complete integration of e.g. a complex molecular diagnostic workflow or a multicellular organ-on-a-chip on a single consumable, has become reality. However the development process is still characterized by complexity which translates into long development times, residual technological risk and resulting cost. In order to streamline the development process and effectively utilize resources, we have developed a modular toolbox strategy comprising generic functional modules which can be combined in a Lego-like fashion in order to rapidly conduct proof-of-concept experiments. We have extended this approach also to instrumentation and assay development. Examples for both devices and instrumentation from a variety of application fields will be presented. Confirmed: Holger Becker, Co-founder and Chief Scientific Officer, microfluidic ChipShop GmbH, Germany

10:30-11:45 Morning Refreshments Room: RENAISSANCE & GRAND SALON

Even Numbered Poster Presentation Sessions One-to-One Partnering Meetings

11:45-12:10 Non-contact Micro/Nano Object Manipulation Recently our research group has developed an innovative method for capturing, concentrating, manipulating and sorting populations of particles, cells, macro-molecules, etc. This novel technique, called Rapid Electrokinetic Patterning (REP), combines features of optical trapping and dielectrophoresis in an innovative, dynamic way using a simple electrode configuration. Transparent electrodes comprised of Indium Tin Oxide (ITO) on glass substrates are used to generate an electric field in the fluid while at the same time allowing light into and out of the fluid. Near-IR optical illumination causes subtle localized heating, creating an electric permittivity gradient that in turn drives a microscopic toroidal vortex. The vortex efficiently transports particles to a preferred location, usually the surface of the electrode. I will discuss applications featuring bacteria, DNA and nanowires. Confirmed: Steve Wereley, Professor of Mechanical Engineering, Birck Nanotechnology Center, Purdue University, USA

12:10-12:35 Acoustofluidics: merging acoustics and microfluidics for biomedical applications The past two decades have witnessed an explosion in lab-on-a-chip research with applications in biology, chemistry, and medicine. The continuous fusion of novel properties of physics into microfluidic environments has enabled the rapid development of this field. Recently, a new lab-on-a-chip frontier has emerged, joining acoustics with microfluidics, termed acoustofluidics. Here we summarize our recent progress in this exciting field and show the depth and breadth of acoustofluidic tools for biomedical applications through many unique examples, from 3D bioprinting to cell-cell communications, from circulating tumor cell isolation and detection to ultra-high-throughput blood cell separation for therapeutics, from high-precision micro-flow cytometry to portable yet powerful fluid manipulation systems. These acoustofluidic technologies are capable of delivering high-precision, high-throughput, and high-efficiency cell/particle/fluid manipulation in a simple, inexpensive, cell-phone-sized device. More importantly, the acoustic power intensity and frequency used in these acoustofluidic devices are in a similar range as those used in ultrasonic imaging, which has proven to be extremely safe for health monitoring during various stages of pregnancy. As a result, these methods are extremely biocompatible; i.e., cells and other biospecimen can maintain their natural states without any adverse effects from the acoustic manipulation process. With these unique advantages, acoustofluidic technologies meet a crucial need for highly accurate and amenable disease diagnosis (e.g., early cancer detection) as well as effective therapy (e.g., transfusion and cancer immunotherapy). Confirmed: Tony Jun Huang, Professor and The Huck Distinguished Chair in Bioengineering Science and Mechanics, The Pennsylvania State University, USA

Agenda: Day One – Monday July 11th 2016


[email protected] +44 (0) 1865 849841

12:35-1:00 Clinical and Environmental Diagnostics Enabled by Paper-Based Microfluidics Paper-based analytical devices have garnered significant attention as a result of their potential ease of use, low cost, and ease of disposability for a number of applications ranging from human health to environmental monitoring. This talk will focus on recent developments from the Henry group on use of porous microfluidic devices for diagnostics applications. For environmental diagnostics, detection of metals and reactive organic compounds will be discussed in the context of personal exposure assessment where porous microfluidics provides unique capabilities in terms of rapid response and sensitive analysis in an affordable package. For clinical diagnostics, recent efforts in adapting peptide nucleic acid assays to paper-based assays for rapid bacteria and virus detection. Confirmed: Charles Henry, Professor, Department of Chemistry, Colorado State University, USA

1:00-1:15 Solution Provider Presentation Integrating Lab-on-chip Automation Early in Product Development Lab on Chip product development is often frustrated by a lack of reproducible performance. Translating a multi-step assay into a microfluidic device without control of the processes occurring in the chip confounds data interpretation. In this presentation, we show how the science of microfluidics is enhanced by early engineering integration of automated control using a simple instrument platform. Confirmed: Leanna M. Levine, PhD, President, ALine, Inc., USA

1:15-2:25 Lunch Room: RENAISSANCE & GRAND SALON

Afternoon chair: Collin Edington, Postdoctoral Researcher, Massachusetts Institute of Technology, USA

2:25-2:50 Inertial microfluidics for isolating, enriching and counting of cells Inertial microfluidics shows tremendous potential for high-throughput cell separations in biomedicine. The approach relies on hydrodynamic forces to manipulate cells in microfluidic channels for size-based focusing and ordering. The interaction of these forces on cells in flowing fluid leads to lateral migration across streamlines into specific cross-sectional positions depending on their size, shape, and deformability. Recent studies demonstrated these effects in microchannels of various geometries, including straight, spiral and serpentine channels. This presentation will summarize our recent work in this emerging field. Our goal is to sort complex cellular samples in devices that allow easy integration with downstream detection. Ultimately, the unique separation functionalities of inertial microfluidics broaden applications of microfluidics in sorting of complex cell mixtures, from cell biology to cancer theranostics. Confirmed: Ian Papautsky, Professor, Department of Electrical Engineering and Computing Systems, University of Cincinnati, USA

2:50-3:15 High-throughput in vivo drug screening platforms using large-scale microfluidics Next generation drug screening will benefit greatly from in vivo studies using small animal models such as C. elegans for hit identification and lead optimization. To enable both high-throughput and high-resolution imaging of multiple C. elegans populations, we recently developed an automated and large-scale microfluidic screening platform. Using this platform, we screened more than 100,000 animals and tested the efficacy of ~1,000 FDA approved drugs in improving an aggregation phenotype of Huntington's disease model. Our robust platform now enables the usage of various C. elegans disease models to carry out high-content screens at the speed and cost of in vitro cell-based assays. Confirmed: Adela Ben-Yakar, N. Doug Williams Memorial Centennial Endowed Faculty, Professor, The University of Texas at Austin, USA

3:15-3:40 3D Multilevel microfluidics: when simple microfabrication technologies widen fluid and cell handling approaches Microfluidics is a flat land. Indeed, since the inception of the field in the early nineties, microflows have been mostly restricted to two dimensions (2D). There are at least two reasons for that. First, microfabrication techniques are historically planar fabrication processes. The second reason is more obvious: why trying to achieve complex three dimensional (3D) networks when simple, 2D devices perform well enough? We propose a generic microfabrication based on roll lamination of photosensitive films enabling a fast, simple and robust level by level fabrication with excellent alignment accuracy on a wide range of hard or flexible substrate. Based on this approach, various functionalities as cell sorting, cell focusing, cell tagging have been performed within multifunctional devices. Confirmed: Anne-Marie Gué, Research Director, LAAS-CNRS, France

3:40-4:35 Afternoon Refreshments Room: RENAISSANCE & GRAND SALON

Odd Numbered Poster Presentation Sessions One-to-One Partnering Meetings

4:35-5:00 Capillary microfluidics by rapid prototyping

Capillary microfluidics on Si chips for immunoassays

Capillary microfluidics in 1D: from continuous flow on thread to digital capillary fluidics on strings

Capillary microfluidic circuits by 3D printing and rapid prototyping for rapid bacterial detection Confirmed: David Juncker, Professor, Micro & Nano Bioengineering Lab, McGill University, Canada


[email protected] +44 (0) 1865 849841

Agenda: Day One continued – Monday July 11th 2016

5:00-5:25 Patent Law Basics: Best Practices for Researchers and Academics

What is a patent? Understanding the different types and the content within patent applications

Identification of the necessary contributions and responsibilities associated with becoming a named inventor on a patent

Patent Application Best Practices: With recent changes in patent law, are lab notebooks still important? Confirmed: Joseph Valentino, PhD, Associate, Fish & Richardson P.C., USA

5:25-5:50 Microdroplet technologies for high-throughput experimentation in biology and for the point-of-care

Droplet microfluidic systems allow to encapsulate and isolate chemical and biological processes inside the pico- to -micro-liter droplets.

One interesting avenue of recent development is in building automated systems that generate and manipulate microscopic droplet reactors over extended periods of time. These systems can be used to e.g. study the evolution in hundreds of independent cultures of microorganisms over extended periods of time providing fascinating new tools for the research on emergence of drug resistance in fluctuating chemical environments.

The physics of flow at small scale can also be used to construct systems that execute complicated protocols on multiple microdroplets completely passively, with minimum requirement for precision in handling, opening these systems for use in point-of-care settings and simplifying common laboratory protocols.

Confirmed: Piotr Garstecki, Professor, Group Leader, Institute of Physical Chemistry, Polish Academy of Sciences, Poland

5:50-6:15 Closing Keynote Address: Droplet Microfluidics – Enabling Technology for High Throughput Screening Analysis Droplet microfluidics enables high throughput screening analysis by utilizing nanoliter-sized drops as mobilized test tubes. High throughput screening is in high demand in a wide range of applications such as material synthesis, drug compound screening, and life science research. Both gas-liquid and two immiscible liquids systems have been extensively studied and developed while this talk will primarily focus on liquid-liquid droplet microfluidics. Specifically, this talk will discuss fundamental transport phenomena of droplet generation, trapping and sorting; physical modeling of droplet generation in different geometries and droplet sorting in T-junctions; and finally electrical sensing mechanisms such as capacitance and microwave sensing. Applications of droplet microfluidics for single encapsulation of particles or beads and for rapid mixing via passive and active means will be briefly mentioned. Confirmed: Carolyn Ren, Professor, Canada Research Chair in Droplet Microfluidics and Lab-on-a-Chip Technology, Department of Mechanical and Mechatronics Engineering, University of Waterloo, Canada

6:15 Chair’s Closing Remarks and End of Day 1

6:15-7:15 Networking Drinks Reception Room: RENAISSANCE & GRAND SALON

Venue Hilton Philadelphia City Avenue 4200 City Avenue Philadelphia, PA 19131 USA A discounted group rate is available to all attendees. Details of how to book are available on registration. Space is limited and accommodation is available on a first come basis.



[email protected] +44 (0) 1865 849841


8:00-8:35 Coffee and Networking Meetings Room: RENAISSANCE & GRAND SALON

8:35-8:40 Room: VERSAILLES Chair’s Welcome Address: Tania Konry, Assistant Professor, Department of Pharmaceutical Sciences, Northeastern University, USA

8:40-9:15 Keynote Address: Extraction and detection of sparse pathogens from biological fluids at the microfluidics scale The molecular diagnosis of blood infections is challenging because pathogens exist in the bloodstream in very low concentrations. Consequently, such sparse target populations typically require DNA extraction and purification from large volume biofluids. This work utilizes the advances in microfluidic technologies to demonstrate initially whether pathogens are present. If so, then a second phase of DNA extraction and identification of the specific pathogens present is performed. Magnetic bead assays and current-wire manipulation enable the initial sensing step. Immiscible phase filtration (IPF) for nucleic acid purification and electrowetting-on dielectric (EWD) droplet actuation are combined on a hybrid microfluidic device that translates from large volume sample-to-small-volume analysis. After IPF reduces the sample volume from a milliliter-sized lysate to a microliter-sized eluent, EWD can be used to automatically prepare the PCR mixture. The extent of purification obtained per IPF wash, and hence the number of washes needed for uninhibited qPCR, are determined via on-chip UV absorbance. The performance of on-chip qPCR, particularly the copy number to threshold cycle correlation, is characterized. Lastly, the above developments accumulate to an experiment that includes the following on-chip steps: DNA purification by IPF, PCR mixture preparation via EWD, and target quantification using qPCR - thereby demonstrating the core procedures in the proposed approach. Confirmed: Richard Fair, Lord-Chandran Professor of Engineering, Department of Electrical and Computer Engineering, Duke University, USA

9:20-9:55 Keynote Address: New Tools for the Isolation of Markers found in Liquid Biopsies: Microfluidic Systems for the Efficient Isolation of CTCs, Cell Free DNA and Exosomes Liquid biopsies are generating great interest within the biomedical community due to the wealth of diagnostic information they contain and the minimally invasive nature of securing important markers to realize precision medicine. These circulating markers consist of whole cells such as circulating tumor cells (CTCs), molecules such as cell free DNA (cfDNA) and nanovesicles such as exosomes. We are developing microfluidic systems that can process whole blood directly and select all three of these markers from a single blood sample. The microfluidic devices are made from plastics via injection molding so that they can be produced in a high production mode and at low-cost to accommodate in vitro diagnostic applications. In this presentation, the fabrication and operational characteristics of these devices for selecting diseased biomarkers from blood samples will be discussed. In particular, we will discuss the use of these markers for managing a variety of cancer-related diseases. Confirmed: Steven Soper, Professor, Department of Biomedical Engineering and Department of Chemistry, University of North Carolina, Chapel Hill, USA

9:55-10:20 Lab-on-a-chip for PET radiopharmaceuticals Microfluidic devices offer great potential for synthesis and analysis of radiopharmaceuticals used for PET imaging, moving away from centralised production of PET tracers to dose-on-demand production nearer the patient. Confirmed: Nicole Pamme, Professor in Analytical Chemistry, University of Hull, UK

10:20-11:25 Morning Refreshments Room: RENAISSANCE & GRAND SALON

Odd Numbered Poster Presentation Sessions One-to-One Partnering Meetings

11:25-11:55 Microfluidic Nebulization Platform for Pulmonary Drug and Gene Delivery We demonstrate an acoustofluidic nebulization platform for pulmonary drug and gene delivery, particularly that of a DNA vaccine against influenza. The technology is also a rapid, efficient and straightforward means for synthesizing 100 nm biodegradable polymeric particles within which therapeutic molecules such as nucleic acids, proteins and peptides can be encapsulated. Finally, the ability to synthesize multiple polyelectrolyte coatings encapsulating these biomolecules is shown as a fast and efficient alternative to conventional layer-by-layer assembly. The low cost, particle size control, low power requirement, delivery efficiency, and miniaturizability altogether suggests the platform constitutes an attractive alternative to current nebulizers and inhalers, which we envisage could comprise the next-generation of devices that will revolutionize pulmonary drug and gene delivery for needle-free vaccination therapeutics. Confirmed: Leslie Yeo, Professor of Chemical Engineering & Australian Research Council Future Fellow, School of Engineering, RMIT University, Australia

11:55-12:20 Non-invasive monitoring of cellular activity using integrated microfluidic devices Microfluidic devices and microsystems has emerged as a versatile tool to revisit many biological processes either for better understanding these processes or to intervene and to control various biological activities of cells or within cells. During this talk, a few microfluidic- based systems compatible with most of detection systems for investigation cellular activities are presented. The emphasis will be on the automation, quantitative, real-time and non-invasive measurements of cellular activities. We extend this talk to our research towards developing microfluidic platforms for rare cell separation and culturing as well as for cell neogenesis and cell secretome analysis. Confirmed: Maryam Tabrizian, Professor, Guggenheim Fellow in Biomedical sciences, McGill University, Canada


[email protected] +44 (0) 1865 849841

Agenda: Day Two – Tuesday July 12th 2016

12:20-12:45 Microfluidics for formulation of poorly soluble therapeutics The formulation of poorly aqueous-soluble therapeutic materials into drug delivery systems is an ongoing challenge. While numerous methods exist for improving the disposition of lipid-soluble materials, therapeutics that are insoluble due to a high degree of self-association (typically characterized by higher melting temperatures and crystallinity) are a particular problem. When attempting to incorporate these therapeutics into carrier matrices, such as PLGA, conventional bulk methods fail due to the nearly immediate precipitation of the material when the dispersed phase (therapeutic with PLGA in organic solvent) contacts the aqueous bulk phase. XRD studies post manufacture by microfluidics reveal that for this otherwise highly crystalline compound there is a lack of crystallinity when entrapped in the PLGA matrix. Drug-containing microspheres remain physically stable for over one month at RT. In vitro release studies at 37°C will be presented. Confirmed: Eric Forssen, Principle Scientist and Manager Drug Delivery Sciences, Allergan PLC, USA

12:45-1:10 Diagnosing Disease on a Microchip The transformative growth in microelectronics in the latter half of the 20th century was fueled fundamentally by the ability to simultaneously miniaturize and integrate complex circuits onto monolithic chips. My research aims to harness these same electrical engineering approaches, which have enabled the microelectronic revolution, to solve high impact problems in medical diagnostics. To accomplish this goal my lab develops hybrid microchips, where microfluidics (i.e. micrometer sized plumbing) are built directly on top of semiconductor chips. Confirmed: David Issadore, Assistant Professor, Bioengineering, Electrical and Systems Engineering, University of Pennsylvania, USA

1:10-2:10 Lunch Room: RENAISSANCE & GRAND SALON

Afternoon Chair: Changchun Liu, Research Assistant Professor, Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, USA

2:10-2:35 Centrifugal Microfluidics for Point-of-care Diagnostics We report various examples of fully integrated "lab-on-a-disc" for point-of-care diagnostics such as pathogen specific DNA extraction to test infectious diseases, enzyme-linked immuno-sorbent assay (ELISA) for multiple biomarker detection, and isolation and analysis of circulating tumor cells starting from whole blood. Integration with centrifugal microfluidic technology allows us more precise control of fluids while also reducing the expensive reagent consumption, the required analysis time and possible handling errors. Confirmed: Yoon-Kyoung Cho, Assistant Professor in Biomedical Engineering, UNIST and Group Leader in Center for Soft and Living Matter, IBS, Korea

2:35-3:00 Microfluidic characterization of neutrophil extracellular traps (NETs) formation Neutrophils are essential for innate immunity; a poor control of their activation or abnormalities associated with their function or life cycle play an important role in the development of numerous pathologies such as autoimmunity, chronic inflammation, infections caused by intracellular pathogens, and cancer. In this talk I will show results of a microfluidic device we engineered to characterize the temporal response of the formation of neutrophil extracellular traps (NETs). Confirmed: José García-Cordero, Assistant Professor, Bio-ARTS Lab, Cinvestav-IPN, Monterrey, Mexico

3:00-3:25 Microfluidic synthesis of polymeric microfibers for bioengineering applications We have developed a set of microfluidic methods for the controlled synthesis of monodisperse polymeric microfibers where the size, shape, morphology, spatial composition and the encapsulation of cargos can be precisely tailored. One novel application of microfibers is in the development of a new class of porous hydrogel materials. We show, for the first time, that hydrogels can be produced from the flow-induced gelation of microfiber suspensions. As we are able to tune the physical and chemical properties of the fibers, as well as encapsulate different cargos, such as drugs and cells, in the fiber structure, we propose that these microfiber suspensions are potentially useful material candidates for in situ scaffold fabrication in bioengineering applications. Confirmed: Janine Nunes, Associate Research Scholar and Lecturer, Department of Mechanical and Aerospace Engineering, Princeton University, USA

3:25-3:55 Afternoon Refreshments Even Numbered Poster Presentation Sessions

End of Conference

Agenda: Day Two continued – Tuesday July 12th 2016


[email protected] +44 (0) 1865 849841

Additional Training Courses – Sunday, July 10th

Additional Training Courses – Sunday July 10th, Philadelphia

Course Details

Course 1: Microfluidics and Lab-on-a-Chip Technologies for commercial product

development: Strategies, Technologies, Markets and Applications

Instructor: Holger Becker, Co-founder and Chief Scientific Officer, microfluidic

ChipShop GmbH

Sunday, July 10th 4:00-6:00 PM

Course 2: Basic Principles in Lab-on-a-Chip (LOC) Technologies for the Isolation of

Circulating Markers: Application in Liquid Biopsies

Instructor: Steven Soper, Professor, Department of Biomedical Engineering and

Department of Chemistry, University of North Carolina, Chapel Hill

Sunday, July 10th 7:00-9:00 PM

Cost:

For prices and registration, please visit

http://www.globalengage.co.uk/microfluidics/usa/register.html

Venue:

The training courses will take place at the conference venue

(Hilton Philadelphia City Avenue)

Outline of Courses:

A full outline of each course can be found below. To book your place on either

course please contact Global Engage.

http://www.globalengage.co.uk/microfluidics/usa/register.html

Learning Objectives

Understand the role of microfluidics technology in the development of new products.

Learn about development and modularisation strategies in product development.

Understand different microfabrication methods for low and high volume production.

Understand economic aspects in the development and manufacturing of Lab-on-a-chip devices and systems.

Learn about examples of successful and unsuccessful microfluidic product introductions.

Understand the current state of the markets and obstacles in the commercialization process.

Get an overview on current trends in LOC research Topics and Course Organisation

Introduction

Challenges in product development

Case studies

Commercialization issues

Materials and microfabrication methods

Application and products

Design Advice

Conclusions

Training Course: Microfluidics and Lab-on-a-Chip Technologies for

commercial product development: Strategies, Technologies, Markets

and Applications

Holger Becker

Co-founder and CSO, microfluidic ChipShop GmbH

Sunday, July 10th, 4:00 – 6:00 PM

Training Course: Microfluidics and Lab-on-a-Chip Technologies for commercial product development: Strategies, Technologies, Markets and Applications

Sunday, July 10th, 2016 4:00-6:00 PM

Who Should Attend?

The course will provide a broad overview of Lab-on-a-Chip (LOC) technologies as an

enabling technology for new product development in diagnostics and the life sciences.

Emphasis is put on the complete development process for commercial microfluidics-

enabled products, covering aspects of development strategies, manufacturing

technologies, application cases, markets as well as aspects of commercialisation and

latest trends in the academic world. Recent product examples will be presented as well

as lessons learned during all stages of the development and commercialization process

of LOC-enabled devices.

Dr Holger Becker is co-founder and CSO of microfluidic ChipShop GmbH. He obtained physics degrees from the University of Western

Australia/Perth and the University of Heidelberg and obtained a PhD in Physics from University Heidelberg in 1995. Between 1995 and

1997 he was a Research Associate at Imperial College with Prof. Andreas Manz. In 1998 he joined Jenoptik Mikrotechnik GmbH. Since

then, he founded and led several companies in the field of microsystem technologies in medicine and the life sciences, for which he received

various awards. He led the Industry Group of the German Physical Society between 2004 and 2009, and is the current chair of the SPIE

‘‘Microfluidics, BioMEMS and Medical Microsystems’’ conference, co-chair of MicroTAS 2013 and Industrial Committee Chair for MicroTAS

2016. He serves on the Editorial Board of “Lab-on-a-Chip”, “Microelectronic Engineering” as well as acting as a regular reviewer of

project proposals on a national and international level.

Training Course: Basic Principles in Lab-on-a-Chip (LOC) Technologies

for the Isolation of Circulating Markers: Application in Liquid Biopsies

Prof. Steven A. Soper

NIH Biotechnology Resource Center of Biomodular

Multi-scale Systems for Precision Medicine

Departments of Biomedical Engineering and Chemistry

University of North Carolina, Chapel Hill

Sunday, July 10th, 7:00 – 9:00 PM

For detailed description, please see below

Training Course: Basic Principles in Lab-on-a-Chip (LOC) Technologies for the Isolation of Circulating Markers

Sunday, July 10th, 2016 7:00-9:00 PM

Purpose: The goal of this short course is to introduce participants

into the burgeoning area of liquid biopsies and the technologies

used for the isolation of the markers (Circulating Tumor Cells, CTCs,

cell-free DNA, cfDNA, and exosomes) directly from whole blood

comprising the liquid biopsy. The major technology platform that

will be discussed includes lab-on-a-chip (LOC) platforms. This short

course will be broken down into two general areas: (1)

Operational characteristics of LOC platforms and their

appropriateness for selecting rare markers from whole blood; and

(2) Application of LOC technologies for the selection of rare

circulating biomarkers from whole blood. The topical areas in each

section are outlined below.


[email protected] +44 (0) 1865 849841

Prof. Soper is a Professor in Biomedical Engineering and Chemistry at the University of North Carolina, Chapel Hill. He also

holds an appointment at Ulsan National Institute of Science and Technology in Ulsan, South Korea. Prof. Soper is currently an

Associate Editor for the Analyst and on the Editorial Board for Journal of Fluorescence and Journal of Micro- and

Nanosystems. Prof. Soper is also serving as a permanent member of the Nanotechnology study panel with the National

Institutes of Health. His research efforts are focused on developing transformative tools for making health-related

measurements at unprecedented speeds with full process automation directly at the Point-of-Care. As a result of his efforts,

Prof. Soper has secured extramural funding totaling >$60M and has published over 305 manuscripts (h index = 58) and is

the author of 12 patents. He is also the founder of a startup company, BioFluidica, which is marketing devices for the

isolation and enumeration of circulating tumor cells.

(1) Basic fundamentals of LOC platforms

a. Brief introduction to the analytical challenges with the isolation of circulating markers from whole blood:

CTCs, cfDNA and exosomes

b. Introduction to LOC technologies; what can they provide with respect to the analysis of rare circulating

markers and comparison to benchtop procedures.

c. Materials for LOC

d. Fabrication technologies

i. Micromilling

ii. Lithography

iii. Dry/wet etching

iv. Molding

e. Metrology and fluid characteristics in microfluidics

i. Electrokinetic versus hydrodynamic flow

ii. Basic fluid and particle (cell) transport

(2) Application of LOC technologies for the isolation of circulating markers from whole blood.

a. Basic components of the liquid biopsy

i. CTCs

1. What is a CTC including different types?

2. Clinical information from CTCs

3. Benchtop techniques for the isolation of CTCs

ii. cfDNA

1. Characteristics of cfDNA

2. How much circulating tumor DNA is found in whole blood?

3. What clinical information can be garnered from cfDNA?

4. Qiagen-based techniques for isolating cfDNA

5. Isolation from plasma is required

iii. Exosomes

1. What is an exosome and how is it generated?

2. Physical characteristics of exosomes

3. Ultra-centrifugation methods for isolating exosomes

4. Why is it necessary to isolate exosomes from plasma?

b. LOC platforms for isolating plasma from whole blood

i. Skimming technologies

ii. Filtering based approaches

iii. Throughput – how much blood is necessary to search for rare markers?

c. LOC platforms for the analysis of CTCs

i. Biological-based techniques

1. Sinusoidal chip

2. Micro-pillar based chip with herringbone mixer

3. Nano-pillar chips for isolating CTCs

4. iChip

ii. Physical-based techniques

1. Microfluidics using dielectrophoresis

2. Size-based separation using inertial lift forces or micro-pores

d. LOC platforms for the analysis of cfDNA

i. Techniques that use magnetic beads

ii. Solid-phase extraction of cfDNA

e. LOC platforms for the analysis of exosomes

i. Centrifugation-based techniques

ii. Affinity selection of disease-specific exosomes

1. Micro-pillar technologies

2. Solid-phase affinity selection

Training Course: Basic Principles in Lab-on-a-Chip (LOC) Technologies for the Isolation of Circulating Markers

Sunday, July 10th, 2016 7:00-9:00 PM


[email protected] +44 (0) 1865 849841

Title Principal Author(s) Affiliation

1 High-throughput Microfluidic Fabrication of Synthetic Asymmetric Vesicles to Support Membrane Biology Studies

Li Lu, Jeffrey W. Schertzer, and Paul R. Chiarot

State University of New York at Binghamton

2 Point-of-Care (POC) Micro Biochip for Cancer Diagnostics Bharath Babu Nunna Shiqiang Zhuang & Eon Soo Lee

New Jersey Institute of Technology

3 Sliding manifolds for instrument-free pneumatic control of microfluidic devices

Stefano Begolo, Paul Said Ehrlich, Justin Podczerviensky, Leanna Levine

ALine Inc.

4 Reducing Surface Adsorption of Analytes in Digital Microfluidics

Sergio L. S. Freire, Katee O’Malley, Caitlyn McConnell

University of the Sciences

5 Live single cell – level glycoanalysis Chandra K Dixit, James Rusling University of Connecticut

6 Micro-Flow Calibration Facility at NIST James W. Schmidt, John D. Wright

National Institute of Standards and Technology

7 Acoustic bubbles for microfluidic sensing enhancement

Andrea De Vellis, Dmitry Gritsenko, Yang Lin, Zhenping Wu, Xian Zhang, Yayue Pan, Wei Xue and Jie Xu

University of Illinois at Chicago

8 Escherichia coli Chemotaxis: Microfluidic experiments and Lattice –Boltzmann modelling to Simulate Cell Motility

Hakan Başağaoğlu, Spring Cabiness, Alexander J. Carpenter, Justin Blount, John R. Harwell (Southwest Research Institute); Hoa Nguyen, Cameron McCay, Frank Healy (Trinity University)

Southwest Research Institute, Trinity University

9 Microfluidic Chip with Hierarchical Micro-nano Patterns for Effective Isolation of Circulating Tumor Cells

Yaling Liu, Shunqiang Wang, Chris Uhl, Wentao Shi

Lehigh University

10 Measuring dielectric properties from individual particles using isomotive dielectrophoresis

Daniel Allen and Stuart J. Williams University of Louisville (Louisville, Kentucky, USA)

11 A large-scale on-chip droplet incubation chamber enables equal microbial culture time

Jing Dai, Hyun Soo Kim, Adrian Guzman, Won-Bo Shim, Arum Han

Texas A&M University

12 Investigation of dielectric and structural properties of live and heat-killed bacteria by broadband impedance spectroscopy in a microfluidic chip

Hang Li, Caroline Multari, Cristiano Palego, Xiao Ma, Xiaotian Du, Yaqing Ning, James C. M. Hwang, Xuanhong Cheng

Lehigh University, Bethlehem, Pennsylvania, United States

13 Optimization of nanoparticle focusing by coupling thermophoresis and engineered vortex in a microfluidic channel

Chao Zhao, Zhibo Cao, John Fraser, Alparslan Oztekin and Xuanhong Cheng

Lehigh University

14 Numerical simulation of cancer cell squeezing through microfluidic channels

Jifu Tan, Yaling Liu Lehigh University

15 Development of a “smart” electroporation-on-a-chip system for high throughput, single cell transfection

Mingde Zheng, Joseph J. Sherba, Jerry W. Shan, Hao Lin, David I. Shreiber, Jeffrey D. Zahn

Departments of Biomedical Engineering and Mechanical & Aerospace Engineering, Rutgers, The State University of New Jersey

16 Nanoparticles focusing in a microfluidic thermal gradient device

Zhibo Cao, Chao Zhao, Alparslan Oztekin, Xuanhong Cheng

Lehigh University, Bethlehem, PA, United States

17 Measurement of Temperature and Pressure with Fiber Bragg Gratings in Microfluidic Devices

Gregory A. Cooksey, Zeeshan Ahmed

National Institute of Standards and Technology

18 Microfluidic blood-brain barrier model provides in vivo-like barrier properties for drug permeability screening

Ying I. Wang, Hasan Erbil Abacia and Michael L. Shuler

Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University

19 Fractal Electrodes to Enhance Dielectrophoresis Anil Koklu, Ahmet C. Sabuncu, Ali Beskok

Southern Methodist University

20 Engineering of biomimetic 3D porous microvascular network Jie Lu a,b , Ying I. Wang* a, Michael L. Shuler* a

a Cornell University, Ithaca, NY, USA b Southeast University, Nanjing, Jiangsu, China

21 Effective Mixing in Droplet Microfluidics Utilizing a Microwave Chaotic Mixer

Gurkan Yesiloz, M. Said Boybay, Carolyn L. Ren

University of Waterloo

22 Pumpless surface microfluidic device for live-cell imaging of β-cell for human transplant.

A Ghosh, JE Medoza-Elias, M Elsharkawy, Y Wang, J Oberholzer, C Megaridis

University of Illinois, Chicago


[email protected] +44 (0) 1865 849841

Poster Presentations

23 Improved Performance of Microfluidic CE-SDS Protein Purity Analysis through the use of Real-Time Optimization of Destain Currents

Jingjing Wang, Derek Troiano, Laurel Provencher, David Weinberger, Brian Gerwe and Rick Bunch

PerkinElmer, Inc. Waltham, MA USA

24 Electrostatic Generation and Ratcheted Transport of Aqueous Droplets

Charles A. Cartier, Jason R. Graybill, Kyle J. M. Bishop

The Pennsylvania State University

25 MRM-MS Detection of Cancer-Relevant Phosphopeptides from Miniaturized Platforms

Jingren Deng, Iulia M. Lazar Virginia Tech

26 Electroporation of brain endothelial cells on chip toward transcellular permeabilization of the blood-brain barrier

Mohammad Bonakdar, Elisa Wasson, Rafael V. Davalos

Virginia Polytechnic Institute and State University

27 The Princeton Microfluidics Laboratory Jason Puchalla Princeton University

Global Engage – Other Related Events

2ND Microfluidics Congress – October 20-21, 2016, London, UK http://www.globalengage.co.uk/microfluidics.html 2nd qPCR & Digital PCR Congress USA – July 11-12, 2016, Philadelphia, USA http://www.globalengage.co.uk/digital-and-qpcr.html 4th qPCR & Digital PCR Congress – October 20-21, 2016, London, UK http://www.globalengage.co.uk/qpcr.html 2nd Microscopy Congress – November 14-15, 2016, London, UK http://www.globalengage.co.uk/microscopy.html Mass Spectrometry Congress – November 14-15, 2016, London, UK http://globalengage.co.uk/mass-spectrometry.html

Poster Presentations


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Poster Presentations Continued

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