Semen cryopreservation of Marwari stallions at farmer’s door
Yash Pal, R. A. Legha, A. Arangasamy and R. C. Sharma
Equine Production CampusNational Research Centre on Equines,
Bikaner – 334 001 (Rajasthan).
Semen processing & freezing
• Semen collection and gel removal
• Microscopic and physical examinations
• Adding media and Centrifugation
• Decanting supernatant and adding freezing media
• Filling and sealing of straws with semen
• Freezing in planner
• Plunging in LN2 for storage
• Thawing at 370C for 30 second for AI
UDAIPUR
Details of Stallion
Day Total Vol.
Gel Free Vol.
Motility (%)
Post thaw motility (%)
Dose
Raj Rattan
165 Cm
Skew bald
Day 1
10 ml 10 ml 60-70 20-25
40Day 2
100 ml 70 ml 70-80 30-40
Day 3
40 ml 25 ml 60-70 30-40
Day 4
100 ml 48 ml 60-70 30-40
Details of Stallion
Day Total Vol.
Gel Free Vol.
Initial Motility(%)
Post thaw motility(%)
Dose
Ganesh
152 Cm
Dark Bay
Day 1 10 ml 10 ml 80-90 40-5040
Day 2 40 ml 35 ml 70-80 40-50
Day 3 40 ml 25 ml 70-80 40-50
Day 4 50 ml 45 ml 50-60 40-50
• Raj Tilak, 158 cm, Pie bald
• Semen could not be freezed.
• PT motility of less than 5 % were observed.
DUNDLODName of Stallion
No. of Collection
Pre freezing motility (%)
Post-Thaw Motility (%)
Semen Dose
BahadurShah
Grey 161cm
4 70-80 40-45 25
Hans Raj
Grey 155cm
4 70-80 35-40 20
Diwana
Bay 157cm
3 60-70 25-30 10
Gaj Raj
Brown 155cm
3 70-80 30-35 18
HansrajBahadurshah
StallionDAY
Total gel free semen volume
Sperm Motility (%)
Pot-thaw motility (%)
Semen doses
Raj rajeswar 1 - - - -
159 cm 2 35 ml 50-60 5-15 -
Skew bald 3 25 ml 60-70 30-40 5
4 45 ml 70-80 40-50 12
5 20 ml 70-80 35-45 6
Total semen doses 23
LADNU
Raj sekhar didn’t donated semen in AV
Results of AI at EPC farmSr. no.
Name of Mare Stallion No. of AI
Result of AI
1 Waheeda Hansraj 2 Pregnant
2 Ruchi Rajrattan 2 Pregnant MF
3 Heer Ganesh 2 Pregnant
4 Hemlata Ganesh 2 Pregnant
5 Hina Ganesh 1 Pregnant
6 Madhuri Ganesh 2 Pregnant FF
7 Krishna Rajrattan 3 Pregnant FF
8 Simmi Ganesh 2 Pregnant
9 Rajiya Rajrattan 3 Pregnant