semen cryopreservation of marwari stallions at farmer’s door

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Semen cryopreservation of Marwari stallions at farmer’s door Yash Pal, R. A. Legha, A. Arangasamy and R. C. Sharma Equine Production Campus National Research Centre on Equines, Bikaner – 334 001 (Rajasthan).

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Page 1: Semen cryopreservation of Marwari stallions at farmer’s door

Semen cryopreservation of Marwari stallions at farmer’s door

Yash Pal, R. A. Legha, A. Arangasamy and R. C. Sharma

Equine Production CampusNational Research Centre on Equines,

Bikaner – 334 001 (Rajasthan).

Page 2: Semen cryopreservation of Marwari stallions at farmer’s door
Page 3: Semen cryopreservation of Marwari stallions at farmer’s door
Page 4: Semen cryopreservation of Marwari stallions at farmer’s door
Page 5: Semen cryopreservation of Marwari stallions at farmer’s door

Semen processing & freezing

• Semen collection and gel removal

• Microscopic and physical examinations

• Adding media and Centrifugation

• Decanting supernatant and adding freezing media

• Filling and sealing of straws with semen

• Freezing in planner

• Plunging in LN2 for storage

• Thawing at 370C for 30 second for AI

Page 6: Semen cryopreservation of Marwari stallions at farmer’s door
Page 7: Semen cryopreservation of Marwari stallions at farmer’s door

UDAIPUR

Details of Stallion

Day Total Vol.

Gel Free Vol.

Motility (%)

Post thaw motility (%)

Dose

Raj Rattan

165 Cm

Skew bald

Day 1

10 ml 10 ml 60-70 20-25

40Day 2

100 ml 70 ml 70-80 30-40

Day 3

40 ml 25 ml 60-70 30-40

Day 4

100 ml 48 ml 60-70 30-40

Page 8: Semen cryopreservation of Marwari stallions at farmer’s door

Details of Stallion

Day Total Vol.

Gel Free Vol.

Initial Motility(%)

Post thaw motility(%)

Dose

Ganesh

152 Cm

Dark Bay

Day 1 10 ml 10 ml 80-90 40-5040

Day 2 40 ml 35 ml 70-80 40-50

Day 3 40 ml 25 ml 70-80 40-50

Day 4 50 ml 45 ml 50-60 40-50

Page 9: Semen cryopreservation of Marwari stallions at farmer’s door

• Raj Tilak, 158 cm, Pie bald

• Semen could not be freezed.

• PT motility of less than 5 % were observed.

Page 10: Semen cryopreservation of Marwari stallions at farmer’s door

DUNDLODName of Stallion

No. of Collection

Pre freezing motility (%)

Post-Thaw Motility (%)

Semen Dose

BahadurShah

Grey 161cm

4 70-80 40-45 25

Hans Raj

Grey 155cm

4 70-80 35-40 20

Diwana

Bay 157cm

3 60-70 25-30 10

Gaj Raj

Brown 155cm

3 70-80 30-35 18

Page 11: Semen cryopreservation of Marwari stallions at farmer’s door

HansrajBahadurshah

Page 12: Semen cryopreservation of Marwari stallions at farmer’s door

StallionDAY

Total gel free semen volume

Sperm Motility (%)

Pot-thaw motility (%)

Semen doses

Raj rajeswar 1 - - - -

159 cm 2 35 ml 50-60 5-15 -

Skew bald 3 25 ml 60-70 30-40 5

4 45 ml 70-80 40-50 12

5 20 ml 70-80 35-45 6

Total semen doses 23

LADNU

Raj sekhar didn’t donated semen in AV

Page 13: Semen cryopreservation of Marwari stallions at farmer’s door

Results of AI at EPC farmSr. no.

Name of Mare Stallion No. of AI

Result of AI

1 Waheeda Hansraj 2 Pregnant

2 Ruchi Rajrattan 2 Pregnant MF

3 Heer Ganesh 2 Pregnant

4 Hemlata Ganesh 2 Pregnant

5 Hina Ganesh 1 Pregnant

6 Madhuri Ganesh 2 Pregnant FF

7 Krishna Rajrattan 3 Pregnant FF

8 Simmi Ganesh 2 Pregnant

9 Rajiya Rajrattan 3 Pregnant

Page 14: Semen cryopreservation of Marwari stallions at farmer’s door
Page 15: Semen cryopreservation of Marwari stallions at farmer’s door