Bio-Connect B.V. T NL +31 (0)26 326 44 50 T BE +32 (0)2 503 03 48 Begonialaan 3a F NL +31 (0)26 326 44 51 F BE +32 (0)2 503 03 27 6851 TE Huissen E [email protected] The Netherlands W www.bio-connect.nl
Allele Biotech, Product Guide
Interest in any of the products,
request or order them at Bio-Connect.
Product Guide
Now including Orbigen
llele
Allele Biotechnology 9924 Mesa Rim Road San Diego, CA 92121 Phone: (858)587 - 6645 Website: www.allelebiotech.com Email: [email protected]
Additional Services
Next Day Oligo Service
1 to 45 0.05
46 to 70 0.05
1 to 45 0.2
46 to 70 0.2
70-100 0.2
>100 0.2
>150 0.2
>200 0.2
1 to 45 1
46 to 70 1
>70 1
Number of bases Synthesis scale
24 (858)587-6645 - www.allelebiotech.com 1 (858)587-6645 - www.allelebiotech.com
Table of Contents
Fluorescent Proteins 2
Polynucleotide Preparation 3
Molecular Cloning 7
Baculovirus Expression 9, 15
Retrovirus Expression 17
Cell Culture / Assay 18
Protein Analysis 19
Equipment / Misc 21
Additional Products/Services 23
To place an order, visit our website or call us at (858)587-6645. For any additional questions, comments, or concerns, email us at [email protected].
Biotechnology
llele
Introducing cost effectiveness to research
Induced Pluripotent Stem Cells 11
Fluorescent Proteins
2 (858)587-6645 - www.allelebiotech.com 23 (858)587-6645 - www.allelebiotech.com
Additional Products/Services
* More than 3-fold brighter than ECFP and EGFP* More than twice as photostable as ECFP/EGFP* Single emission peak* Single-exponential fluorescence lifetime ( = 3.2 ns)* Can be co-imaged with red and yellow FPs* Low sensitivity to acidic pH(fluorescence pKa=4.3)* Mammalian expression vector ready to transfect your favorite cells* True monomer that will not aggregate or cause non-specific interactions
Type/quantity Quantity
mWasabi 10 ug
mTFP1 10 ug
Features:
Academic or government institutions:Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such licensing specifically prohibits the right to sell or otherwise transferthis product or its components to a third party.
Industry For-Profit Institutions:Institutions that wish to use this product in non-commercial or commercial applications are required to obtain a sub-licensing agreement from Allele Biotech. For sub-licensing information please visit Fluorescence Protein Sublicense, or contact us via phone or email.
Licensing:
Antibodies in the areas of Apoptosis, cell surface molecules/GPCRs, cytokines, chemokines, growth factors, genetic disease markers, kinases and phosphatases, neuronal proteins, oncoproteins, proteases, and transcription factors. Also, a unique collection of high-quality pan-specific and phospho-specific antibodies is offered.
Antibodies
The protein expression custom service team of Allele Biotech, now including Orbigen, provides services at all stages of the protein expression, production, and purification processes from gene cloning to large-scale fermentation. We are on the cutting edge of a variety of expression and antibody production technologies so that you can accelerate your research by taking advantage of our comprehensive range of services.
Protein Expression Services
For additional information, visit www.allelebiotech.com or call us at (858)587-6645
All ele quip Chilling/Heating Dry Bath
MK-20
All ele quip Chilling/Heating Dry Bath
MS100-4P
All ele quip Bacti-Cinerator Sterilizer
HM-3000
Ampicon ThermoMini500 Dry Bath
AM500
Ampicon ThermoExp500 Thermocycler
ATE500
All ele -Blue DNA Gel Loading Dye (6X)
6 ml ABP-PP-GLDB001
All ele -Red DNA Gel Loading Dye (6X)
6 ml ABP-PP-GLDR001
Type/quantity Catalog Number
All ele Mini-Prep Plasmid DNA Purification kit
50 rxns ABP-PP-MP00500 100 rxns ABP-PP-MP01000 250 rxns ABP-PP-MP02500
All ele -In-One PCR/Gel Purification kit
50 rxns ABP-PP-PCRG100 100 rxns ABP-PP-PCRG250 250 rxns ABP-PP-PCRG050
Cells & Tissue Genomic DNA Preparation
50 rxns ABP-PP-GNCT050 100 rxns ABP-PP-GNCT100 250 rxns ABP-PP-GNCT250
Baculovirus Genomic DNA Preparation
50 rxns ABP-PP-GNBV050 100 rxns ABP-PP-GNBV100 250 rxns ABP-PP-GNBV250
Equipment / Misc
22 (858)587-6645 - www.allelebiotech.com 3 (858)587-6645 - www.allelebiotech.com
Polynucleotide Preparation
Type/quantity Catalog Number
Allelequip H2O3 Ice-Temp Incubator 48 (0~4oC)
H2O3-48
Allelequip H2O3 Ice-Temp Incubator 96 (0~4oC)
H2O3-96
Allelequip H2O3 Ice-Temp Incubator 75 (0~75oC)
H2O3-75
Allelequip Compact High Speed Centrifuge
MK12KMK14K
Allelequip MiniFuge Centrifuge
M-6K
Allelequip ThermoShaker (5oC~99oC)
MS-100
Allelequip ThermoShaker, (0oC~99oC)
MSC-100
Type/quantity Catalog Number
Polynucleotide Preparation
4 (858)587-6645 - www.allelebiotech.com 21(858)587-6645 - www.allelebiotech.com
Equipment / Misc
Allele-In-One Mouse Tail Direct Lysis Buffer
100 rxns ABP-PP-MT01100500 rxns ABP-PP-MT01500
Allele-In-One Mouse Tail Direct PCR Kit
100 rxns ABP-PP-MT02100500 rxns ABP-PP-MT02500
Allele-In-One Human Blood Direct Lysis Buffer
100 rxns ABP-PP-BD01100500 rxns ABP-PP-BD01500
Allele-In-One Human Blood Direct PCR Kit
100 rxns ABP-PP-BD02100500 rxns ABP-PP-BD02500
Type/quantity Catalog Number
Mouse tail biopsy at ~0.3cm was incubated in 100µL Lysis Buffer for 2 hours at 55oC. Genomic DNA in 1µl lysate was directly amplified in a 10µL PCR reaction (5µL of 2X PCRMastermix added) for 29 cycles.
Protein Analysis
20 (858)587-6645 - www.allelebiotech.com 5 (858)587-6645 - www.allelebiotech.com
Polynucleotide Preparation
All ele Extract-I Protein Reagent (Tris Buffered)
125 uL ABP-PA-AXIR125T 250 uL ABP-PA-AXIR500T
All ele Extract-I Protein Reagent (PBS Buffered)
100 rxns ABP-PA-AXIR125P 500 rxns ABP-PA-AXIR500P
ProGuard Protein Stabilizing Reagent
2 ml AGL-PA-PGSR012 10 ml AGL-PA-PGSR052
All ele Membrane Protein Isolation Kit
1 kit AGL-PA-MT101JB
AquaBlue Protein Stain
0.5L AGL-PA-AQBL005 1L AGL-PA-AQBL010
Type/quantity Catalog Number
1.1X PCR MasterMix Solution, 96 rxns 10 µL ABP-PP-MM11961 50 µL ABP-PP-MM11965
2X PCR MasterMix Solution, 96 rxns 10 µL ABP-PP-MM02961 50 µL ABP-PP-MM02965
Taq DNA Polymerase, 5 units/µl
100 U ABP-PP-ERTQ100 200 U ABP-PP-ERTQ200 500 U ABP-PP-ERTQ500 1000 U ABP-PP-ERTQ001
High Quality dNTP Mix, 10mM
1 mL ABP-PP-ERDN100 5 mL ABP-PP-ERDN500
dATP, 100mM
100 uL ABP-PP-ERDA100 500 uL ABP-PP-ERDA500
dTTP, 100mM
100 uL ABP-PP-ERDT100 500 uL ABP-PP-ERDT500
Type/quantity Catalog Number
AlleleBalanced Luciferase Assay Kit
100 rxns ABP-PA-ABLA0011000 rxns ABP-PA-ABLA011
AlleleExtract-B Protein Reagent (Tris Buffered)
125 mL ABP-PA-AXBR125T500 mL ABP-PA-AXBR500T
AlleleExtract-B Protein Reagent (PBS Buffered)
125 mL ABP-PA-AXBR125P500 mL ABP-PA-AXBR500P
AlleleExtract-M Protein Reagent (Tris Buffered)
125 mL ABP-PA-AXMR125T500 mL ABP-PA-AXMR500T
AlleleExtract-M Protein Reagent (PBS Buffered)
125 mL ABP-PA-AXMR125P500 mL ABP-PA-AXMR500P
Type/quantity Catalog Number
dCTP, 100mM
100 uL ABP-PP-ERDC100500 uL ABP-PP-ERDC500
dGTP, 100mM
100 uL ABP-PP-ERDG100500 uL ABP-PP-ERDG500
10x PCR Buffer with MgCl2
1x1 ml ABP-PP-ERBFM012x1 ml ABP-PP-ERBFM025x1 ml ABP-PP-ERBFM05
10x PCR Buffer without MgCl2
1x1 ml ABP-PP-ERBF0012x1 ml ABP-PP-ERBF0025x1 ml ABP-PP-ERBF005
MgCl2 Solution (100 mM)
1 ml ABP-PP-ERMGS01
Type/quantity Catalog Number
Polynucleotide Preparation
6 (858)587-6645 - www.allelebiotech.com 19 (858)587-6645 - www.allelebiotech.com
Protein Analysis
AvantGene Transfection Reagent
125 uL ABP-TC-ATR125U 250 uL ABP-TC-ATR250U 750 uL ABP-TC-ATR750U
All ele Profect Protein Delivery Reagent Kit
24 vials AGL-PA-APDK024 96 vials AGL-PA-APDK096
Type/quantity Catalog Number
Transfection of GFP plasmid into rat muscle cells using AvantGene
High concentration T4 DNA Ligase, 10X Buffer
100 U ABP-CE-T4L10X1 500 U ABP-CE-T4L10X5
High concentration T4 DNA Ligase, 2X Buffer
100 U ABP-CE-T4L2X01 500 U ABP-CE-T4L2X05
T4 DNA Polymerase
100 U ABP-CE-T4PO100 500 U ABP-CE-T4PO500
T4 Polynucleotide Kinase
100 U ABP-CE-TPK100U 1000 U ABP-CE-TPK1000
CleanCut Protease, 10 Units/uL
500 U ABP-CE-CCP0500
Type/quantity Catalog Number
Cell Culture / Assay
18 (858)587-6645 - www.allelebiotech.com 7 (858)587-6645 - www.allelebiotech.com
Molecular Cloning
DH5α Extreme Efficiency Competent Cells
5 X 60uL ABP-CE-CC0200520 X 60uL ABP-CE-CC0202050 X 60uL ABP-CE-CC0205096 X 60uL ABP-CE-CC02096
High Efficiency Competent Cells SC33
5 X 60uL ABP-CE-CC0100520 X 60uL ABP-CE-CC0102050 X 60uL ABP-CE-CC0105096 X 60uL ABP-CE-CC01096
Xtreme Efficiency DH10β Electrocompetent Cells
5 X 60uL ABP-CE-XDHE00520 X 60uL ABP-CE-XDHE02550 X 60uL ABP-CE-XDHE050
Type/quantity Catalog Number
Comparison of SC33 HE cells (left)with competent cells of a commonlyused competitor. One aliquot of cellswas used for standard transformation with 1x10^-11 g of pUC19 plasmid.
PhoenixTM Retroviral Expression System
1 Kit ABP-RVK-10001
pBMN-Z Retroviral Expression Vector
10 µg ABP-PVL-10011
pBMN-mTFP1 Retroviral Expression Vector
10 µg ABP-PVL-10014
Phoenix TM Ampho Cells ( Human )
1vial ABP-RVC-10001
Phoenix TM Eco Cells ( Mouse )
1vial ABP-RVC-10002
Type/quantity Catalog Number
The Phoenix™ Retroviral Expression System offers the most time-tested method for producing infectious, replication-incompetent retrovirus that can efficiently transfer genes into a variety of mammalian cell types in vitro or in vivo.
Molecular Cloning
8 (858)587-6645 - www.allelebiotech.com 17(858)587-6645 - www.allelebiotech.com
Retrovirus Expression
Type/quantity Catalog Number
TNM-FH Insect Culture Medium
1 liter ABP-MED-10001
Serum-Free Insect Culture Medium
1 liter ABP-MED-10002
Serum Free, Met Free Insect Culture Medium
1 liter ABP-MED-10003
Grace’s Insect Medium (Unsupplemented)
1 liter ABP-MED-10004
Baculovirus expression systems and accessories, ready-to-use baculovirus high-titer viral stocks, insect cell lines and media, retroviral expression system, and reagents for RNA interference.
Baculovirus Expression
16 (858)587-6645 - www.allelebiotech.com 9 (858)587-6645 - www.allelebiotech.com
Baculovirus Expression
Type/quantity Catalog Number
Sapphire™ Insect Transfection Kit
25 rxns ABP-BVD-10003
5x Insect Lysis Buffer (with Protease Inhibitor)
10 ml ABP-BUF-10010
pVL1392-XylE Control Vector Kit
1 kit ABP-BVP-10007
XylE Staining Solution
1 liter ABP-BUF-10020
pVL1392-mTFP1 Control Vector Kit
1 kit ABP-BVP-1000
Sapphire™ Baculovirus DNA
5 µg ABP-BVD-10001
Sapphire™ Transfection Kit
1 kit ABP-BVD-10002
pVL1392 Vector
10 µg ABP-BVP-10001
pVL1393 Vector
10 µg ABP-BVP-10002
pORB Vector
10 µg ABP-BVP-10003
pAcSec1 Vector
10 µg ABP-BVP-10004
pAcIRES Vector
10 µg ABP-BVP-10005
Sf9 Insect Cells (Culture)
1 x 107 cells ABP-CEL-10002
Sf21 Insect Cells (Culture)
1 x 107 cells ABP-CEL-10003
T.ni Insect Cells (Culture)
1 x 107 cells ABP-CEL-10005
Sf9 Insect Cells (Frozen)
1 x 107 cells ABP-CEL-10006
Sf21 Insect Cells (Frozen)
1 x 107 cells ABP-CEL-10007
T.ni Insect Cells (Frozen)
1 x 107 cells ABP-CEL-10008
Type/quantity Catalog Number
Baculovirus Expression
Type/quantity Catalog Number
10 (858)587-6645 - www.allelebiotech.com 15(858)587-6645 - www.allelebiotech.com
Baculovirus Expression
Human OSKM Lentiviral Paticles Set
4 vials ABP-SC-LVIOSKM
Human OSKMP Lentiviral Particles Set
5 vials ABP-SC-LVIOSKMP
All-In-One-Vector: OSKM Lentiviral Paticles
5 vials ABP-SC-LVI4IN1
Human OSKMPH Lentiviral Particles Set
6 vials ABP-SC-LVIOSKMPT
Mix: Human OSKM Lentiviral Particles Mix
5 vials ABP-SC-VILEOSK
Human OSNL Lentiviral Particles Set
4 vials ABP-SC-LVIOSNL
Human OSNLP Lentiviral Particles Set
5 vials ABP-SC-LVIOSNLP
Type/quantity Catalog Number
Induced Pluripotent Stem Cells
Type/quantity Catalog Number
14 (858)587-6645 - www.allelebiotech.com 11(858)587-6645 - www.allelebiotech.com
Induced Pluripotent Stem Cells
HiTiter™ pLICO OSKM Vector Set
10ug x 4 ABP-SC-VCLEOSK
HiTiter™ pLICO OSNL Vector Set
10ug x 4 ABP-SC-VCLEOSN
pCHAC OSKM Retrovirus Vector Set
10ug x 4 ABP-SC-RHOSKM4
pCHAC OSNL Retrovirus Vector Set
10ug x 4 ABP-SC-RHOSNL4
bFGF-LIF-Producing MEF Feeder Cells
20 vials ABP-SC-BLIFM20
bFGF Human Fibroblast Feeder Cells
20 vials ABP-SC-BFFDH20
bFGF-Producing MEF Feeder Cells
20 vials ABP-SC-BFFDM20
Validated hSOX2 Lentiviral Particles
5 vials ABP-SC-LVISOX2
Validated hTERT Lentiviral Particles
5 vials ABP-SC-LVITERT
Validated p53 shRNA Lentiviral Particles
5 vials ABP-SC-LVIP53S
Human OSKM Retroviral Particles Set
4 vials ABP-SC-VIREOSK
Human OSNL Retroviral Particles Set
4 vials ABP-SC-VIREOSN
Validated hc-MYC Retroviral Particles
5 vials ABP-SC-VIREMYC
Validated hKLF4 Retroviral Particles
5 vials ABP-SC-VIREKLF
Type/quantity Catalog Number
Induced Pluripotent Stem Cells
Type/quantity Catalog Number
12 (858)587-6645 - www.allelebiotech.com 13(858)587-6645 - www.allelebiotech.com
Induced Pluripotent Stem Cells
Human OSNLPH Lentiviral Particles Set
6 vials ABP-SC-LVIOSNLPT
Human OSKMP Lentiviral Particles Set
5 vials ABP-SC-LVIOSKMP
Validated hc-MYC Lentiviral Particles
5 vials ABP-SC-LVICMYC
Validated hKLF4 Lentiviral Particles
5 vials ABP-SC-LVIKLF4
Validated hLIN28 Lentiviral Particles
5 vials ABP-SC-LVILN28
Validated hNANOG Lentiviral Particles
5 vials ABP-SC-LVINANO
Validated hOCT3/4 Lentiviral Particles
5 vials ABP-SC-LVIOCT3