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Produk Indigenous Sebagai Pangan Fungsional
Fransiska Rungkat Zakaria
Materi 8
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Body needs both omega 3 and 6
Linoleiclinolenic
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Jenis Asam Lemak MinyakKelapa
MinyakKelapa
Minyak IntiSawit
(%)a Sawit (%)b (%)b
Asam Kaprat 4,9 – 9,5
Asam Kaprilat 5,5 – 9,5 ! – 4
Asam Kaproat "," – ",# ! – $
Asam Larat 44," – 5&," 4' – 5&
Asam Miristat !," – 9," , – &,5 4 – $
Asam almitat $,5 – ",5 4" – 4' ',5 – 9
Asam almitoleat "," – !,
Asam Stearat ,"–
!," !,'–
4,$ –
&,5
Asam *leat 5," – #," !9 – 45 ! – 9
Asam Linoleat ,5 – &,5 $ – ",5 &
Asam Arak+ionat "," –",4
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• Contoh:
tempe, tahu, oncom, bir pletok, wedang jahe, cincau,tape beras, peujeum, dodol, kredok, urap, asinan,
sayur asin, kacang rebus, tauge sayuran dan buah-
buahan tropis, bahan lalapan, rumput laut, dsbnya
DEFII!I
"angan tradisional #E$#%&% '()%':
#ahan baku atau resep makanan dan minuman yang terbuat dari bahan-bahan
yang terdapat di Indonesia dan telah dikenal sejak dahulu*
"angan +ungsional
akanan atau minuman yang berpengaruh positi+ terhadap kesehatan,
)egiatan +isik dan mental, disamping kandungan at-at giinya
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"angan +ungsional.+unctional +ood "angan rekayasa.deigner +ood
"harma+oods utraceuticals
"angan tradisional Fungsional.#iologis /
Fungsional:
Fungsi biologis seluler: sintesis protein, enim, hormon,
D%, $%, senyawa metabolik
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0enis sel: endotelium, hati dan organ-organ lain, darah merah,
imun, syara+, kulit, penglihatan, dsbnya
!istem: 1askuler, imun, hormon, syara+, pencernaan
regenerasi.pertumbuhan sel 2kanker3, energi
)esehatan:
4anggung jawab bersama
Ilmu dan teknologi pangan dengan +armakologi
dan kedokteran
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Paradigma sehatParadigma sehat
SEHAT
SAKIT MENJADI
SEMBUH
=
=TIDAK SAKIT
TETAP TIDAKOBAT
MAKANAN
(FUNGSIONAL
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"endahuluan: &ubungan antara makanan dan tubuh
Eat What Your Body is Made For
Cells dlm
organ tubuh
"angan.bahan alami:
segar, *olahan
etabolisme
2pembongkaran3
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WHO 2002
"encegahan "erawatan
"engobatan
SEHAT
SEHAT
SAKIT
-SA.A
KEDOKTERANFARMASI
-SA.APENCEGAHAN:
PANGAN,
NATURAL PRODUCTS
PARADIGMA
SEHAT
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World Cancer Research Funds (WCRF) & AmericanInstitute for Cancer Research (AICR) 199! "0 halaman
It is a#undantl$ clear that the incidence of allthe common cancers in human is #ein%
determined #$ arious 'otentiall$ controlla#leeternal factors
*his is surel$ the most comfortin% fact to comeout of all cancer research+ for it means that
cancer is+ in lar%e 'art+ a 'reenta#le diseases
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,an%an-ma.anan-#ahan
na#ati-natural 'roducts $an% da'atmence%ah .an.er da'at mence%ah'en$a.it /antun%+
'em#uluh darah+ dia#etes+dan 'en$a.it de%eneratiflainn$a
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,an%an-ma.anan $an% da'at mence%ah .an.er
er#a%ai /enis sa$uran
Antosianin
lorofilserat
Karotenoid,
terpenpoid
Daun cincau
Flavonoid/fenolik
Isotiosianat
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a%aimanater/adin$a .an.er
85% .e/adian .an.er dise#a#.anoleh fa.tordari luar tu#uh!arsino%en (,olusi ma.anan -3inuman-udara-air)+ sinar 45+ irus+ infe.si
Han$a 15% dise#a#.an oleh .eturunan
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Polusi udara, pencemaran makanan, UV/sinar matahari,,kegemukan, hormon
Gen
Gen
Polusi udara,pencemaranmakanan,Uvsinarmatahari,,virus,infeks:85%
eturunan:15%i
!el " Gen#ormal
!el " gen$idak normal
Gen
Gen
Gen
Gen
Gen
Gen
a%aimana ter/adin$a .an.er
d . .
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!el tidak normal,gen tidak normal,menghasilkan aringan kanker/tumor
36*A7*A76
3686AR
,olusi! ma.ananminuman
udara+ air+ sinar+
.e%emu.an+hormon
a%aimana ter/adin$a .an.er
GenGen Gen Gen
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:i'ern%aruhi oleh!; A.tiitas fisi.+
.e%emu.an+ma.anan
$an% di.onsumsi; Hormon dan
fa.tor
'ertum#uhan:i'ern%aruhi oleh!
;
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"angan 4anaman obat
5at gii:
protein,lemak,karbohidrat, 1itamin,mineral, serat
!umber utama
----------------
)omponen+ungsional.bioakti+
on-gii)adar rendah )adar tinggi
$asa, te6tur, 1olume,
)emungkinan toksik
"enting
!angat kecil
tidak penting
#esar
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7Functionality8 berdasarkan komponen:
)omponen 0enis Fungsi !umber
5at-at gii makro "rotein, lemak,karbihidrat
5at pembangun,Energi, pelindung
Esensial
#iji-bijian,)acang-kacangan,
Daging, ikan,
dll
9itamin dan
ineral esensial
%, #, C, D, E, ),Folat, pantotenat,
iasin, biotin
etabolisme
!eluler normal
Esensial
!ayuran dan buah-buahan,
$umput laut,
sintetik
!erat !elulosa, pektin,
hemiselulosaum, oligoskarida
"rebiotik,
)ontrol kolesterol,
"encernaan,ImunitasDia betes, kanker,kegemukan
!ayuran dan
buah-buahan,$umput laut
!intetik
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)omponen 0enis Fungsi !umber
ula alkohol
Eritritol
%rabitol, ribitol,6ilitol
!orbitol,manitol,
!ebaian prekursor glikogen,antiketogenik, substitusi gularendah kalori, kambah, la6ati1e,
%nti caries, anti tumor
anggang, jamur,
E6udat tanaman,molases, rumputlaut
%sam amino, %rginin
%spartat.gin
lutamat
4ripto+an
4irosin
Fenilalanin
%ntihipertensi
Fatig kronik, sirosis hati
%nti epilepsis
%nti insomnia
%nalgesik
%nti depresi. "arkinson;sdiseases
%nti depresi.hiperakti+
"rotein
7Functionality8 berdasarkan komponen:
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)omponen 0enis Fungsi !umber
"eptida Casomorphin
Imunopeptida
Caseinophosphopeptida
"eptida bioakti+
%nti diarea
!timulasi imunitas
%bsorpsi Ca
%ntihipertensi
)asein susu
&idrolisa proteinkacang-kacangan,ikan
#akteri asamlaktat
'actococcus,'actobacillus,
#i+idobacterium,dll
'actose intolerant
"robiotik: diarea, antikolesterol. kanker.konstipasiimunostimulan,
"roduk +ermentasi
!usu, sayuran, dll
"
'%, '%, D&% etabolismearakhidonat, anti penyakit kronis
'emak tanamnDaun, biji-bijian,ikan*
7Functionality8 berdasarkan komponen:
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)omponen 0enis Fungsi !umber
4hioallyl C&?@C&-C&?-A
A@strukturorganik
&ypolipidemic%ntitrombotik
%nti kanker
#awang putih
"rotese inhibitor )unit %nti kanker )edele, kacang-
kacanganChlorophyllins )hloro+ol
tanaman%ntikanker )hloro+ol
tanaman
'ignans %ntikanker
Estrogen
)edle, gandum
"EI4C2"henethylisothiocyanate3
%ntikanker Cruci+erous
Curcumin I, III Di+eruroilmetan %ntikanker )unyit
7Functionality8 berdasarkan komponen:
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7Functionality8 berdasarkan komponen:
)omponen 0enis Fungsi !umber
)arotenoid %nti penyakit degenerati+
%ntio6idan
!ayuran, buah-
buahan, teh
ingerols,shogaol
%ntio6idan, antiateroskelosis,
"encernaan, %nti kanker
0ahe
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7Functionality8 berdasarkan komponen:
)omponen 0enis Fungsi !umber
Isotiosianat sul+o+ran antikanker brokoli
%cto6ika1ikolasetat
Fenil propanoid %nti kanker Languas galanga
%urapten 2%
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"enggunaan komoditi tanaman pangan sebagai bahan obat 2"(3
#udidaya:
4emulawak 0ahe
'engkuas
)encur
Cengkeh)unyit
#engle
"are
4an hutan:
%lang-alang
'empuyagn wangi
Curcuma 6anthoria5ingiber o++icinale $o6b
'anguas galanga 2'3 !tunt
)aemp+eria galanga '
Eugenia aromatica '* (*)*Curcuma domestica 9al
5ingiber purpureum $o6b
omordica charantina '
Imperata cylindrica 2'3 #eau1
5ingiberis aromaticum 9ahl
>>=?GH
?=
=
=HH==
?>?
H?G
?
G?=?
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"otensi pangan tradisional sebagai pangan +ungsional
#ahan baku:prinsipal.suplemen:
sayuran buah-buahan, rempah-rempah, ganggang,
rumput laut, mikroorganisme, jamur
Diet.menu: gado-gado, asinan, rujak, kredok, bubur manado,
sayur asam, lodeh, sayur tumis, pindang ikan, sop
buntut.kaki ayam, bubur kacang, cincau, wedang
jahe, bir pletok, manisan buah, selai
"erlu standardisasi bahan.pengolahan dan uji khasiat
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Ginger (Zingiber officinale Roscoe) extracts increase in vivo human LDL
resistance to oxidation and prevent in vitro
cholesterol accumulation in mouse
macrophage Fransiska Rungkat-Zakaria(1, Aisya !" #eptiana($ and #ulistiyani(%"
1& Dept Food !ecnology and 'uan )utition,
*ogor Agricultural +niversity, Indonesia$& +niversity of end" #oediran, Indonesia
%& Faculty of at . )atural #ciece,
*ogor Agricultural +niversity, Indonesia
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inger ( Zingiber officinale Roscoe& ri0oa
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INTRODUCTION
GINGER
Zingiber family
The rhizome is commonly accepted as a sorce of
!a"or #coo$ing% drin$s% ba$ing
Gro&s &ell in tropical rainforest climate
Traditional beliefs:
• prevention of common cold, physiological and
stomach disorders,
inammation, diarrhea, etc (Tang and Eisenbrand,
1992)
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ntio!idant capacity of ginger e!tract (oleoresin
fractions) inlinoleic acid system " a#tocopherol ($i%&'a%i and
a%atani,199)
• *leoresin fractions gingerol, shogaol
+&ppress #lipo!ygenase or prostaglandin
synthetase(-lynn and .a/erty, 190)
rotect T ind&ced ear edema, epidermal
ornithine decarbo!ylaseactivity, s%in t&mor promotion in female 34. mice
(ar% et al, 1990)
rotect lymphocytes and hybridoma cells from
'cienti(c Reports
odified D
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odified D
( 23 4 D, Ac D&
D
5agocytosis
5inocitosis
D
Reseptor
ysosoe
receptor
syntesis
Acetate
6olesterol
7ndosoe
#cavenger Reseptor
+6 76
!ranslocation
'D
6olesterol up
take6olesterol
processing
6olesterol
e3clussion
acropage colesterol eta8olis
+6
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D
ipid
o3idation
5rotein
modi+ication +ptake D
R2#
entry
differentiation
acropage
Foa cells
Adesion
onocyte ipid streaks
Initiation ecanis of aterosclerosis
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$E!E%$C& (#0EC4I9E!
• 4o +ind out whether drinking ginger water e6tract can
* 'ower plasma cholesterols
?* "rotect plasma 'D' against o6idation
• 4o +ind out whether the protection o+ 'D' can pre1ent +urther
accumulation o+ cholesterol in macrophage
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E4&(D('L
!ubjects: ? healthy male students li1ing in the same religious dormitory
two groups: treated group and control.placebo group
inger drinks:
• water e6tract, pasteuried HC, added with sugar syrup
• standardied acceptable drink
• gi1en e1ery a+ternoon at G:HH• placebo group recei1ed syrup without ginger e6tract
"lasma analysis:
* 4otal cholesterol 2C&(D-"%" method, #oehringer-annheim, kit3?* 'D'-C 2"9! method, #oehringer-annheim, kit3
>* &D'-C 2phosphotungstic acid3
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'D' isolation:
• ml plasma J ml aC' H*KM J ED4% H*HM• incubation in polyalomer tubes•=*HHH rpm HC, ?H hours• 4ube cutting β 9'D'
'D' J 'D' 2dN *HH= g.ml3
• !caled tubes J H*HK g )#r.ml• i6ing• 4rans+er to new polyaomer tubes J ml )#r in aCl-ED4%• =*HHH HC ? hrs
• 'D' 2upperpart, d , *H=> g.ml3• 'D', dialied
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'D' o6idation:
• 4he isolated 'D' was o6idied with µ Cu!( at >GHC
• conjugated dienes was monitor continuously at ?> nm +or >H minutes
D% 'D' analysis:
• E6traction with butanol• measurement at nm e6itation, > nm emission 2Conti et al
KK3
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6olesterol accuulation in acropages9
• 5lasa +rom one healthy male was incu8ated with >H µg.mlginger dichloromethane e6tract, µ a-tocopherol or none
+or > hrs at >GHC•ouse was injected with thioglycolate• acropages, ?->6H=, isolated +rom peritoneal, were incubated
in $"I =H containing M #!% and incubated +or hrs at>GHC, M C(?• cells were washed and cholesterol was e6tracted with
isopropanol• total cholesterol, esteri+ied cholesterol, +ree cholesterol analysis
was done by &"'C
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$E!
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Before
TREATMENT After
Before
PLACEBO After
HDL!"
LDL!"
T#ta$!"
TG
%
&%
'%
(%
)%
*%%
*&%
*'%
*(%
*)%
m
g d
HDL!"
LDL!"
T#ta$!"
TG
Figure * &D', 'D', and total cholesterol, and triglyceride in plasma o+ male student
subjects be+ore and a+ter treatment with ginger drink +or >H days 2n@?3 and
those in control placebo subjects 2n@?3
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0
100
200
300
00
!00
"00
#00
$00
0 2! !0 #! 100 12!
!ime" min
n m o l c o n # u g a t e d d i e n a m g
L D L
p r o t e i n
0 t%&e of 'o(tro)
30 t%&e of 'o(tro)
0 t%&e of tre*t&e(t
30 t%&e of tre*t&e(t
Figure ?* !igni+icant protecti1e e++ects o+ drinking ginger +or >H days in male
student subjects 2n@?3 on resistance o+ the isolated 'D' against
o6idation by Cu!( +or HH minute, measured by conjugated dienes
as the o6idation product during*
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0
!0
100
1!0
200
2!0
300
3!0
00
!0
!00
Co(tro) D%'+)oro&et+*(e
e,tr*'t
*-To'o.+ero)
Free C+o)e/tero)
E/ter o)e/tero)
Tot*) '+o)e/tero)
Figure >* Cholesterol pro+iles in macrophages incubated with o6idied human'D' that ha1e been supplemented with ginger dichloromethane
e6tract 2>H µg.ml3, α-tocopherol 2 µ3 or unsupplemented 2control3
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62)6+##I2)
1" Drinking ginger :ater e3tract for %; days did not lo:er
8lood colesterol levels nor reduce plasa DA
$" 'o:ever tere :as significant resistance to o3idation of te
D against induced o3idative stress
%" !e resistance of D to o3idation prevent colesterol
accuulation in acropage cells siilar to -tocoperol
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I
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o
"arameters
!ubjects %1erage 1aluesDay H Day >H
!I o+ cells
cultured with
Con %
4reated group
>*>>
2?*=>-=*H3
H*=>
2H*=-H*G3
Control group G*G
2*-H*G3
H*?
2*=>-=*G3? !I o+ cells
cultured with'"!
4reated group *>K
2*HK-*H3
?*HG
2H*>->*>3Control group *G
2H*K->*H=3
?*H
2H*=-=*H3
> !I o+ cells
cultured with by
paraBuat
4reated group *
2H*G-*>3
?*
2H*>-*3
Control group *?
2H*GK-?*>?3
*=?
2H*=?->*K3
D% cells
2µmol.l3
4reated group >*>>
2?*=>-=*H3
H*=>
2H*=-H*G3
Control group >*H>
2?*-*H3
H*>
2H*=G-*H=3
"ercentage o+
CD>J
cells
4reated group ?*?
2GG*G-K*?K3
H*K
=*-K-KK3
Control group GK*?
2=>*G-K>*3
G*
2=K*>-G-=?3
4able* %1erage and range 1alues o+ lymphocyte cell analysis o+ proli+eration acti1ities 2!I3 in
the presence o+ Con %, '"! or paraBuatQ o+ D% cellQ and o+ percentage o+ CD> cells
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%+
*%*+
&%&+,%,+
-#.tr#$ gr#/0
(.=*&
Treated gr#/0
(.=*&
L 1 s i . g
a " t i 2 i t 1 ( 3
*%%4*
+%4*
%
*%
&%
,%
'%
+%
%
-#.tr#$ gr#/0
(.=*&
Treated gr#/0
(.=*&
L 1 s i . g a " t i 2 i t 1
( 3
*%%4*
+%4*
Figure 1*Increasing ) lysing acti1ity o+ ? healthy male subjects gi1en ginger drink**#lood was
withdrawn +or lymphocyte isolation at be+ore treatment 2%3 and a+ter treatment 2#3 +rom control
and treated groups* inger drink was gi1en +or >H days e1ery a+ternoon and the cells were
incubated with target cells which had been treated with &>-thymidine with the ratio o+ HH: or
H:* Data was e6pressed as count per minute o+ the unlysed target cells*
%
#
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%
+
*%
*+
&%
&+
% ,%Da$s o% treatment
MDA #5
treated
s/67e"ts
(/m#$8LMDA #5
"#.tr#$
s/67e"ts
(/m#$8L9itami. E #5
treated
s/67e"ts
(/m#$8L9itami. E #5
"#.tr#$
Figure ?* D% and 1itamin E content in plasma o+ subjects be+ore 2H day3 and a+ter recei1ing ginger
drink +or >H days 2n@?3* 4he subjects were healthy male students li1ing in the same dormitory* D%
was analyed using the method o+ Conti et al 2KK3*9itamin E was analyed using spectro+luorometer*
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*KG ?*HK >*G >*KG * *K =* G*KH * H*?>
Retention tie (in& Retention tie (in&
Retention tie (in&
A *
6Figure"'56 croatogra of ginger oleoresin
(A&, of plasa fro control su8
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CONCLUSSON
! n human study" using ma#e adu#t hea#thy sub$e%ts" there is im&ro'ement in ( %e##s &ro#i)eration a%ti'ities and &er%entage*! m&ro'ement on ( %e##s +as a%%om&anied by resistan%e o) #ym&ho%yte %e##s against
o,idati'e stress* (his im&ro'ement +as demonstrated by the %e##s obtained )rom thesub$e%ts drinking ginger
! Resistan%e o) the #ym&ho%ytes +as not a%%om&anied by de%rease in M-. %e##
! Beside the ro#e o) ginger %om&ounds as &rote%tant against o,idati'e stress" they a&&eared to ha'e other immunoenhan%ement a%ti'ity" notab#y in im&ro'ing N/ %e## a%ti'ity* (his
im&ro'ement in N/ %e## a%ti'ity had been obser'ed in the ma#e student studied re%ei'ingginger drink )or 30 days
! ana#ysis o) ginger substan%es in the asma re'ea# the &resen%e o) the assumed gingero#grou& in the asma o) sub$e%ts drinking ginger" higher than that o) the %ontro# grou&*
! Sin%e N/ %e##s are kno+n to ha'e s&e%i)i% a%ti'ity in #ysing mutated or in)e%ted %e##s"the resu#ts o) this resear%h has sho+n the s%ienti)i% su&&ort o) the traditiona# be#ie)s thatginger %an im&ro'e body resistan%e to %ommon %o#d*
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I!7RA!+R7 R7>I7?
* 4ang O and Eisenbrand * KK?* Chinese Drugs o+ "lant (rigin: Chemistry, "harmacology and
G* Conti , orand "C, 'e1illain ", and 'emonnier %* KK* Impro1ed +luorometric determination o+
malonaldehyde* 0 Clin Chem, >G.G: ?G>-?G
* Frit )', elson 4', $ui-9elasco 9, and ercurio !D* KK* %cute intramuscular injection o+ oils or oleic acid
component protects mice against paraBuat lethality* 0 utr* K: ?-?
K* eydani !, Ou D* !antos ! and &ayek * KK* %ntio6idants and immune response in aged persons:
(1er1iew o+ present e1idence* %m 0 Clin utr* =?: =!-G!
H* 5akaria-$ungkat, F*, urahman, "rangdimurti, E*, 4ejasari* ?HH>* %ntio6idant and Immunoenhancement
%cti1ities o+ inger 2 Zingiber officinale $oscoe3 E6tracts and Compounds in In 9itro and In 9i1o ouseand &uman !ystem* utraceuticals and Foods*Q K=-H
A6K)2?7D77)!
4his reseach was +unded by the
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%)4IFI4%!
%4I )%)E$ E' CIC%< &I0%<
(Cyclea barbata L.Miers)
Fransiska RungkatZakaria,
!ndang "rangdimurti, !dna #nanta, #lbertus $eno "andoyo
&E%'4&L DE!!E$4
%)%% "EC
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"ED%&
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In+ormasi ilmiah mengenai khasiat gel cincau hijau akan mendukung
perkembangan produk pangan +ungsional yang berbentuk 7healthy dessert8,
yang akhir-akhir ini amat digemari karena dapat mengimbangi konsumsi
diet mengandung lemak dan protein hewani tinggi dan kadar serat rendah*
)anker merupakan penyakit yang diawali dari mutasi gen seluler, yang
diketahui sebagian besar disebabkan oleh +aktor e6ternal seperti polutan
kimia baik pada makanan maupun lingkungan, 1irus, dan radiasi*
ekanisme pencegahan mutasi gen dapat terjadi melalui akti+itas
antio6idan, perbaikan imunitas, atau sitotoksik 2OC$FP%IC$, KKGQ
5akaria-$ungkat et al*, ?HH3
E4(D% "EE'I4I%
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E4(D% "EE'I4I%
E6traksi air daun segar dengan aBuades dilakukan secara manual karena
terbentuk gel yang kemudian didinginkan untuk memperoleh cairan sineresis*
E6trak air daun, akar dan batang dikeringbekukan*
E6trak etanol dan he6an dilakukan pada daun, akar dan batang keringbeku*
E6trak di tambahkan pada media kultur sel-sel kanker )=? 2turunan sel
leukimia3 dan &ela 2turunan sel kanker ser1i63 dan pada kultur sel lim+osit
yang diisolasi dari darah tepi seorang mahasiswa sehat, dengan tingkat
konsentrasi: C 2.? C?3Q C? 2setara dengan konsumsi segelas minuman
cincau3, C> 2?6C?3Q C 26C?3Q C 26C?3*
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&'(
(
'(
)((
c) c* c+ c, c'
-onsentrasi
. P e n g h a m b a t a n
/id
etd
hed
aib
etb
heb
aia
eta
hea
ambar * E+ek sitotoksik e6trak air 2ai3, etanol 2et3 dan he6an
2he3 dari daun 2d3, batang 2b3 dan akar 2a3 tanaman cincau hijau
terhadap pertumbuhan sel leukemia 2)=?3* E6trak air akarmempunyai persentase penghambatan diatas =M, air batang
diatas >HM, sedang air daun diatas >GM setelah konsentrasi diatas
C>* "eningkatan konsentrasi N C> 2?6 ? gelas muniman gel
cincau3 tidak nyata menaikkan akti+itas sitotoksik*
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&+(
&*(
&)(
(
)(
*(
+(
,(
'(
c) c* c+ c, c'
-onsentrasi
.
P e n g h a m b a
t a n
/id
etd
hedaib
etb
heb
aia
eta
hea
ambar ?* %kti+itas sitotoksik e6trak air 2ai3, etanol 2et3 dan he6an2he3 dari daun 2d3, batang 2b3 dan akar 2a3 tanaman cincau hijau
terhadap kehidupan sel kanker ser1i6 2&ela3*Ekstrak %ia hingga
konsentrasi C 2setara gelas minuman gel cincau3 menghambat
pertumbuhan sebesar >M, e6trak aid ??M pada konsentrasi C*
4abel %kti+itas sitotoksik 2persen3 e6trak gel cincau pada sel lim+osit manusia
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#a-
pel
61 6$ 6% 6@ 6=
%id -K -? -G - -
Etd -H H -?= R G
&ed K R = >R > R
%ib - -K R ->K R ->Etb -? -? -G -? R
&eb -?? R - -? -G K
%ia > - -G -> R -? R
Eta -K ? -?= R H&ea > - = - G
2R3 menunjukkan perbedaan nyata terhadap kontrol pada selang kepercayaan KM
#ilangan negati+ menunjukkan penurunan nilai absorbansi*
4abel * %kti+itas sitotoksik 2persen3 e6trak gel cincau pada sel lim+osit manusia
yang dikultur dalam media $"I =H lengkap* !el hidup dianalisa dengan 44
4abel ? )adar alkaloid e6trak cincau %nalisa dilakukan dengan
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#a-
pel
5elar
ut
& #copolain & !ropic acid
B 8"8 B 8"k B 8"8 B 8"k
Daun
%ir - - *? >*
Etanol - - H*= H*K&e6an H*? H*>G - -
#ata
ng
%ir H*=K * - -
Etanol - - H* *
&e6an H*HG H* - -
%kar
%ir - - *?> ?*?
Etanol H*>H H*= - -
&e6an - - H*K *?H
4abel ?* )adar alkaloid e6trak cincau* %nalisa dilakukan dengan
&"'C dengan menggunakan asam scopolamin-r dan tropic
sebagai standard*
)E!I"
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)E!I"
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#ogor %gricultural
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I)!R2D+6!I2)
!uour gro:t9o3idative stress, iunological disorder, alnutrition
"otential role +or o6idati1e-induced injury in the cancer process
speci+ically during the promotion stage 2)launig et al, KK3
!ources o+ $(! :
+rom in+lammatory cells 2Cerutti and 4rump, KK3
A need in researc to find suita8le food for cancer patients
reen gel leaf Cyclea barbata "ierscoon refresing drink in #out 7ast Asian countries
kno:n traditionally to ave cooling and anti
inflaation effects in te 8ody
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!is gel /e3tracts of te leaf ad 8een reported to
ave 9
cytoto3ic activity on cancer cell linesnon to3ic on noral uan lypocyte cells
(Zakaria-Rungkat et al, $;;1&
In tis study, te leaves :ere processed to produce po:der
products
:ic included all parts and fi8res in te leaves
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C>& ice n@
Fed :it po:der for
@-:k (1$"1g/kg&
!uor transplantation
!uor latent
period (days&
!uor volue
(oving pen,
!a
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!pleen
#pleen cells
Resistance to
'$2$ (!!&
! cell
(Rosette&
In vitro cytoto3ic to:ard tuor cell
!uor cells
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I>7R
6A!AA#7
6oloetri, #ina 1C$
#2D
(Adenocroe asay
isra" '"5"
#'-5E
(5aglia and >alentine,1C
!otal lutation
D!)*, 7llan" 1G$
DA
(!*A, *uege andAust&
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&ealthy tumor tissue ecrotic tumor tissue
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Figure* 'atent period o+ tumor growth in mice +ed lea+ powder
then implemented with tumor cells 2'"J43 and in miceimplemented with tumor 243* 4here is no di++erence in the latent
periodQ the lea+ did not pre1ent tumor growth in mice
%
%:+
*
*:+
&&:+
,
,:+
LP ; T T S-
L a t e n t p e r i o d " d
a $ s
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%
%:+
*
*:+
&
&:+
0m+
LP ; T T S-
Figure* 4umor 1olume in mice +ed lea+ powder thenimplemented with tumor cells 2'"J43 and in mice
implemented with tumor 243* 4he lea+ powder decrease
tumor 1olume in mice
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%
&
'
G
r
a
m
s LP ; T T S-
Ne"r#se
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%
*%
&%
,%'%
+%
. necrotic
tissue
LP ; T T S-
Figure* "ercentage o+ necrotic tumor tissue in mice +ed
lea+ powder then implemented with tumor cells 2'"J43 and
in mice implemented with tumor 243* 4he lea+ powder
increase the percentage o+ tumor necrotic tissue in mice
! 0ell" .
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Figure* "ercentage o+ 4 cells by rosette techniBue in mice +ed lea+ powder
then implemented with tumor cells 2C"3, implemented with tumor without
the lea+ 243 and in standard control mice 2!C3* Feeding lea+ powder
increased 4cell inspite o+ the tumor growth* 4umor implementations
without the lea+ markedly reduced 4 cell percentage
*(=&
>=+ )=)
-P T S-
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+?,
?,>>
%
*%
&%
,%
'%
+%
%
>%
)%
D
e
a
d
t
u
m
o
r
c
e
l
l
s
"
.
S- T LP;T
Figure* Cytoto6icity o+ 4 cells +rom mice +ed lea+ powder thentransplanted with tumor cells 2'"J 43, with tumor alone and
standard diet 2!C3* !light increase in cytoto6icity in 4 and '"J4,
indicate 4 cell recognition o+ the tumor antigen
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%%%
%+%
*%%
*+%
&%%
&+%
,%%
,+%
'%%
'+%
to weeks markedly decrease cells; resistance to
o6idati1e stress
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MDA
%
+
*%
*+
LP ; T T S-
SOD
%
*%%
&%%
,%%
'%%
+%%
LP ; T T S-
Figure* D% content and !(D acti1ity in mice +ed lea+
powder then implemented with tumor cells 2'"J43Q in mice
implemented with tumor 243 and in mice +ed standard diet*
4he lea+ powder increase !(D acti1ity in mice but reduceD% in the li1er
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%
&%%
'%%
(%%
-ata$ase GSH!P@ GSH
LP ; T T S-
Figure* !& content and antio6idant enyme acti1ity in mice +edlea+ powder then implemented with tumor cells 2'"J43Q in mice
implemented with tumor 243 and in mice +ed standard diet* 4he
lea+ powder reduce li1er catalase acti1ity but did not e++ect the
other enyme acti1ity in the li1er*
C(C'
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C(C'
* 4here is no di++erence in the latent period o+ the tumor growth in
mice* 4he lea+ powder decrease tumor 1olume and tumor mass butincrease tumor necrotic tissue up to > times
?* Feeding lea+ powder increased 4cell inspite o+ the tumor growth*
!light increase in cytoto6icity in 4 and '"J4, indicate 4 cell
recognition o+ the tumor antigen
"rolonged tumor growth +rom > to weeks markedly decrease
cells; resistance to o6idati1e stress
>* 4he lea+ powder increase !(D acti1ity in mice but reduce
D% in the li1er $educe li1er catalase acti1ity but did not
e++ect the other enyme acti1ity in the li1er*
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Lang sudah.sedang kami teliti:
• )ayu manis, cacao
• )umis kucing
• )ecombrang
• 'engkuas, minyak buah merah, Dll
%spek: imunomodulator, anti kanker
anti obesity (baru)
C l i
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Conclusion
•"otensi komoditi lokal sangat besar untuk produk pangan +ungsional
• #ahan baku pangan tradisional mengandungkomponen +ungsional.bioakti+
• )etersediaan bahan berlimpah• "otensi untuk suplemen dari bahan baku pangan,
rempah-rempah, tanaman obat
• $ekayasa pangan +ungsional dari bahan.reseptradisional
• "otensi menu diet tradisional
• asih banyak komoditi yang harus diteliti
T+ 0%dministration #uilding
#ogor agricultural
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T+*(0
1o2
g g y
Indonesia
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