2008 kuliah8 indigenous

8/17/2019 2008 Kuliah8 Indigenous

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Produk Indigenous Sebagai Pangan Fungsional

Fransiska Rungkat Zakaria

Materi 8


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Body needs both omega 3 and 6

Linoleiclinolenic


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Jenis Asam Lemak MinyakKelapa

MinyakKelapa

Minyak IntiSawit

(%)a Sawit (%)b (%)b

Asam Kaprat 4,9 – 9,5

Asam Kaprilat 5,5 – 9,5 ! – 4

Asam Kaproat "," – ",# ! – $

Asam Larat 44," – 5&," 4' – 5&

Asam Miristat !," – 9," , – &,5 4 – $

Asam almitat $,5 – ",5 4" – 4' ',5 – 9

Asam almitoleat "," – !,

Asam Stearat ,"–

!," !,'–

4,$ –

&,5

Asam *leat 5," – #," !9 – 45 ! – 9

Asam Linoleat ,5 – &,5 $ – ",5 &

Asam Arak+ionat "," –",4


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• Contoh:

tempe, tahu, oncom, bir pletok, wedang jahe, cincau,tape beras, peujeum, dodol, kredok, urap, asinan,

sayur asin, kacang rebus, tauge sayuran dan buah-

buahan tropis, bahan lalapan, rumput laut, dsbnya

DEFII!I

"angan tradisional #E$#%&% '()%':

#ahan baku atau resep makanan dan minuman yang terbuat dari bahan-bahan

yang terdapat di Indonesia dan telah dikenal sejak dahulu*

"angan +ungsional

akanan atau minuman yang berpengaruh positi+ terhadap kesehatan,

)egiatan +isik dan mental, disamping kandungan at-at giinya


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"angan +ungsional.+unctional +ood "angan rekayasa.deigner +ood

"harma+oods utraceuticals

"angan tradisional Fungsional.#iologis /

Fungsional:

Fungsi biologis seluler: sintesis protein, enim, hormon,

D%, $%, senyawa metabolik


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0enis sel: endotelium, hati dan organ-organ lain, darah merah,

imun, syara+, kulit, penglihatan, dsbnya

!istem: 1askuler, imun, hormon, syara+, pencernaan

regenerasi.pertumbuhan sel 2kanker3, energi

)esehatan:

4anggung jawab bersama

Ilmu dan teknologi pangan dengan +armakologi

dan kedokteran


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Paradigma sehatParadigma sehat

SEHAT

SAKIT MENJADI

SEMBUH

=

=TIDAK SAKIT

TETAP TIDAKOBAT

MAKANAN

(FUNGSIONAL


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"endahuluan: &ubungan antara makanan dan tubuh

Eat What Your Body is Made For

Cells dlm

organ tubuh

"angan.bahan alami:

segar, *olahan

etabolisme

2pembongkaran3


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WHO 2002

"encegahan "erawatan

"engobatan

SEHAT

SEHAT

SAKIT

-SA.A

KEDOKTERANFARMASI

-SA.APENCEGAHAN:

PANGAN,

NATURAL PRODUCTS

PARADIGMA

SEHAT


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World Cancer Research Funds (WCRF) & AmericanInstitute for Cancer Research (AICR) 199! "0 halaman

It is a#undantl$ clear that the incidence of allthe common cancers in human is #ein%

determined #$ arious 'otentiall$ controlla#leeternal factors

*his is surel$ the most comfortin% fact to comeout of all cancer research+ for it means that

cancer is+ in lar%e 'art+ a 'reenta#le diseases


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,an%an-ma.anan-#ahan

na#ati-natural 'roducts $an% da'atmence%ah .an.er da'at mence%ah'en$a.it /antun%+

'em#uluh darah+ dia#etes+dan 'en$a.it de%eneratiflainn$a


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,an%an-ma.anan $an% da'at mence%ah .an.er

er#a%ai /enis sa$uran

Antosianin

lorofilserat

Karotenoid,

terpenpoid

Daun cincau

Flavonoid/fenolik

Isotiosianat


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a%aimanater/adin$a .an.er

85% .e/adian .an.er dise#a#.anoleh fa.tordari luar tu#uh!arsino%en (,olusi ma.anan -3inuman-udara-air)+ sinar 45+ irus+ infe.si

Han$a 15% dise#a#.an oleh .eturunan


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Polusi udara, pencemaran makanan, UV/sinar matahari,,kegemukan, hormon

Gen

Gen

Polusi udara,pencemaranmakanan,Uvsinarmatahari,,virus,infeks:85%

eturunan:15%i

!el " Gen#ormal

!el " gen$idak normal

Gen

Gen

Gen

Gen

Gen

Gen

a%aimana ter/adin$a .an.er

d . .


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!el tidak normal,gen tidak normal,menghasilkan aringan kanker/tumor

36*A7*A76

3686AR

,olusi! ma.ananminuman

udara+ air+ sinar+

.e%emu.an+hormon

a%aimana ter/adin$a .an.er

GenGen Gen Gen


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:i'ern%aruhi oleh!; A.tiitas fisi.+

.e%emu.an+ma.anan

$an% di.onsumsi; Hormon dan

fa.tor

'ertum#uhan:i'ern%aruhi oleh!

;


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"angan 4anaman obat

5at gii:

protein,lemak,karbohidrat, 1itamin,mineral, serat

!umber utama

----------------

)omponen+ungsional.bioakti+

on-gii)adar rendah )adar tinggi

$asa, te6tur, 1olume,

)emungkinan toksik

"enting

!angat kecil

tidak penting

#esar


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7Functionality8 berdasarkan komponen:

)omponen 0enis Fungsi !umber

5at-at gii makro "rotein, lemak,karbihidrat

5at pembangun,Energi, pelindung

Esensial

#iji-bijian,)acang-kacangan,

Daging, ikan,

dll

9itamin dan

ineral esensial

%, #, C, D, E, ),Folat, pantotenat,

iasin, biotin

etabolisme

!eluler normal

Esensial

!ayuran dan buah-buahan,

$umput laut,

sintetik

!erat !elulosa, pektin,

hemiselulosaum, oligoskarida

"rebiotik,

)ontrol kolesterol,

"encernaan,ImunitasDia betes, kanker,kegemukan

!ayuran dan

buah-buahan,$umput laut

!intetik


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ula alkohol

Eritritol

%rabitol, ribitol,6ilitol

!orbitol,manitol,

!ebaian prekursor glikogen,antiketogenik, substitusi gularendah kalori, kambah, la6ati1e,

%nti caries, anti tumor

anggang, jamur,

E6udat tanaman,molases, rumputlaut

%sam amino, %rginin

%spartat.gin

lutamat

4ripto+an

4irosin

Fenilalanin

%ntihipertensi

Fatig kronik, sirosis hati

%nti epilepsis

%nti insomnia

%nalgesik

%nti depresi. "arkinson;sdiseases

%nti depresi.hiperakti+

"rotein



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"eptida Casomorphin

Imunopeptida

Caseinophosphopeptida

"eptida bioakti+

%nti diarea

!timulasi imunitas

%bsorpsi Ca

%ntihipertensi

)asein susu

&idrolisa proteinkacang-kacangan,ikan

#akteri asamlaktat

'actococcus,'actobacillus,

#i+idobacterium,dll

'actose intolerant

"robiotik: diarea, antikolesterol. kanker.konstipasiimunostimulan,

"roduk +ermentasi

!usu, sayuran, dll

"

'%, '%, D&% etabolismearakhidonat, anti penyakit kronis

'emak tanamnDaun, biji-bijian,ikan*



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4hioallyl C&?@C&-C&?-A

A@strukturorganik

&ypolipidemic%ntitrombotik

%nti kanker

#awang putih

"rotese inhibitor )unit %nti kanker )edele, kacang-

kacanganChlorophyllins )hloro+ol

tanaman%ntikanker )hloro+ol

tanaman

'ignans %ntikanker

Estrogen

)edle, gandum

"EI4C2"henethylisothiocyanate3

%ntikanker Cruci+erous

Curcumin I, III Di+eruroilmetan %ntikanker )unyit



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)arotenoid %nti penyakit degenerati+

%ntio6idan

!ayuran, buah-

buahan, teh

ingerols,shogaol

%ntio6idan, antiateroskelosis,

"encernaan, %nti kanker

0ahe


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Isotiosianat sul+o+ran antikanker brokoli

%cto6ika1ikolasetat

Fenil propanoid %nti kanker Languas galanga

%urapten 2%


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"enggunaan komoditi tanaman pangan sebagai bahan obat 2"(3

#udidaya:

4emulawak 0ahe

'engkuas

)encur

Cengkeh)unyit

#engle

"are

4an hutan:

%lang-alang

'empuyagn wangi

Curcuma 6anthoria5ingiber o++icinale $o6b

'anguas galanga 2'3 !tunt

)aemp+eria galanga '

Eugenia aromatica '* (*)*Curcuma domestica 9al

5ingiber purpureum $o6b

omordica charantina '

Imperata cylindrica 2'3 #eau1

5ingiberis aromaticum 9ahl

>>=?GH

?=

=

=HH==

?>?

H?G

?

G?=?


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"otensi pangan tradisional sebagai pangan +ungsional

#ahan baku:prinsipal.suplemen:

sayuran buah-buahan, rempah-rempah, ganggang,

rumput laut, mikroorganisme, jamur

Diet.menu: gado-gado, asinan, rujak, kredok, bubur manado,

sayur asam, lodeh, sayur tumis, pindang ikan, sop

buntut.kaki ayam, bubur kacang, cincau, wedang

jahe, bir pletok, manisan buah, selai

"erlu standardisasi bahan.pengolahan dan uji khasiat


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Ginger (Zingiber officinale Roscoe) extracts increase in vivo human LDL

resistance to oxidation and prevent in vitro

cholesterol accumulation in mouse

macrophage Fransiska Rungkat-Zakaria(1, Aisya !" #eptiana($ and #ulistiyani(%"

1& Dept Food !ecnology and 'uan )utition,

*ogor Agricultural +niversity, Indonesia$& +niversity of end" #oediran, Indonesia

%& Faculty of at . )atural #ciece,

*ogor Agricultural +niversity, Indonesia


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inger ( Zingiber officinale Roscoe& ri0oa


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INTRODUCTION

GINGER

Zingiber family

The rhizome is commonly accepted as a sorce of

!a"or #coo$ing% drin$s% ba$ing

Gro&s &ell in tropical rainforest climate

Traditional beliefs:

• prevention of common cold, physiological and

stomach disorders,

inammation, diarrhea, etc (Tang and Eisenbrand,

1992)


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ntio!idant capacity of ginger e!tract (oleoresin

fractions) inlinoleic acid system " a#tocopherol ($i%&'a%i and

a%atani,199)

• *leoresin fractions gingerol, shogaol

+&ppress #lipo!ygenase or prostaglandin

synthetase(-lynn and .a/erty, 190)

rotect T ind&ced ear edema, epidermal

ornithine decarbo!ylaseactivity, s%in t&mor promotion in female 34. mice

(ar% et al, 1990)

rotect lymphocytes and hybridoma cells from

'cienti(c Reports

odified D


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odified D

( 23 4 D, Ac D&

D

5agocytosis

5inocitosis

D

Reseptor

ysosoe

receptor

syntesis

Acetate

6olesterol

7ndosoe

#cavenger Reseptor

+6 76

!ranslocation

'D

6olesterol up

take6olesterol

processing

6olesterol

e3clussion

acropage colesterol eta8olis

+6


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D

ipid

o3idation

5rotein

modi+ication +ptake D

R2#

entry

differentiation

acropage

Foa cells

Adesion

onocyte ipid streaks

Initiation ecanis of aterosclerosis


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$E!E%$C& (#0EC4I9E!

• 4o +ind out whether drinking ginger water e6tract can

* 'ower plasma cholesterols

?* "rotect plasma 'D' against o6idation

• 4o +ind out whether the protection o+ 'D' can pre1ent +urther

accumulation o+ cholesterol in macrophage


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E4&(D('L

!ubjects: ? healthy male students li1ing in the same religious dormitory

two groups: treated group and control.placebo group

inger drinks:

• water e6tract, pasteuried HC, added with sugar syrup

• standardied acceptable drink

• gi1en e1ery a+ternoon at G:HH• placebo group recei1ed syrup without ginger e6tract

"lasma analysis:

* 4otal cholesterol 2C&(D-"%" method, #oehringer-annheim, kit3?* 'D'-C 2"9! method, #oehringer-annheim, kit3

>* &D'-C 2phosphotungstic acid3


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'D' isolation:

• ml plasma J ml aC' H*KM J ED4% H*HM• incubation in polyalomer tubes•=*HHH rpm HC, ?H hours• 4ube cutting β 9'D'

'D' J 'D' 2dN *HH= g.ml3

• !caled tubes J H*HK g )#r.ml• i6ing• 4rans+er to new polyaomer tubes J ml )#r in aCl-ED4%• =*HHH HC ? hrs

• 'D' 2upperpart, d , *H=> g.ml3• 'D', dialied


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'D' o6idation:

• 4he isolated 'D' was o6idied with µ Cu!( at >GHC

• conjugated dienes was monitor continuously at ?> nm +or >H minutes

D% 'D' analysis:

• E6traction with butanol• measurement at nm e6itation, > nm emission 2Conti et al

KK3


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6olesterol accuulation in acropages9

• 5lasa +rom one healthy male was incu8ated with >H µg.mlginger dichloromethane e6tract, µ a-tocopherol or none

+or > hrs at >GHC•ouse was injected with thioglycolate• acropages, ?->6H=, isolated +rom peritoneal, were incubated

in $"I =H containing M #!% and incubated +or hrs at>GHC, M C(?• cells were washed and cholesterol was e6tracted with

isopropanol• total cholesterol, esteri+ied cholesterol, +ree cholesterol analysis

was done by &"'C


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$E!


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Before

TREATMENT After

Before

PLACEBO After

HDL!"

LDL!"

T#ta$!"

TG

%

&%

'%

(%

)%

*%%

*&%

*'%

*(%

*)%

m

g d

HDL!"

LDL!"

T#ta$!"

TG

Figure * &D', 'D', and total cholesterol, and triglyceride in plasma o+ male student

subjects be+ore and a+ter treatment with ginger drink +or >H days 2n@?3 and

those in control placebo subjects 2n@?3


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0

100

200

300

00

!00

"00

#00

$00

0 2! !0 #! 100 12!

!ime" min

n m o l c o n # u g a t e d d i e n a m g

L D L

p r o t e i n

0 t%&e of 'o(tro)

30 t%&e of 'o(tro)

0 t%&e of tre*t&e(t

30 t%&e of tre*t&e(t

Figure ?* !igni+icant protecti1e e++ects o+ drinking ginger +or >H days in male

student subjects 2n@?3 on resistance o+ the isolated 'D' against

o6idation by Cu!( +or HH minute, measured by conjugated dienes

as the o6idation product during*


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0

!0

100

1!0

200

2!0

300

3!0

00

!0

!00

Co(tro) D%'+)oro&et+*(e

e,tr*'t

*-To'o.+ero)

Free C+o)e/tero)

E/ter o)e/tero)

Tot*) '+o)e/tero)

Figure >* Cholesterol pro+iles in macrophages incubated with o6idied human'D' that ha1e been supplemented with ginger dichloromethane

e6tract 2>H µg.ml3, α-tocopherol 2 µ3 or unsupplemented 2control3


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62)6+##I2)

1" Drinking ginger :ater e3tract for %; days did not lo:er

8lood colesterol levels nor reduce plasa DA

$" 'o:ever tere :as significant resistance to o3idation of te

D against induced o3idative stress

%" !e resistance of D to o3idation prevent colesterol

accuulation in acropage cells siilar to -tocoperol


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I


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o

"arameters

!ubjects %1erage 1aluesDay H Day >H

!I o+ cells

cultured with

Con %

4reated group

>*>>

2?*=>-=*H3

H*=>

2H*=-H*G3

Control group G*G

2*-H*G3

H*?

2*=>-=*G3? !I o+ cells

cultured with'"!

4reated group *>K

2*HK-*H3

?*HG

2H*>->*>3Control group *G

2H*K->*H=3

?*H

2H*=-=*H3

> !I o+ cells

cultured with by

paraBuat

4reated group *

2H*G-*>3

?*

2H*>-*3

Control group *?

2H*GK-?*>?3

*=?

2H*=?->*K3

D% cells

2µmol.l3

4reated group >*>>

2?*=>-=*H3

H*=>

2H*=-H*G3

Control group >*H>

2?*-*H3

H*>

2H*=G-*H=3

"ercentage o+

CD>J

cells

4reated group ?*?

2GG*G-K*?K3

H*K

=*-K-KK3

Control group GK*?

2=>*G-K>*3

G*

2=K*>-G-=?3

4able* %1erage and range 1alues o+ lymphocyte cell analysis o+ proli+eration acti1ities 2!I3 in

the presence o+ Con %, '"! or paraBuatQ o+ D% cellQ and o+ percentage o+ CD> cells


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%+

*%*+

&%&+,%,+

-#.tr#$ gr#/0

(.=*&

Treated gr#/0

(.=*&

L 1 s i . g

a " t i 2 i t 1 ( 3

*%%4*

+%4*

%

*%

&%

,%

'%

+%

%

-#.tr#$ gr#/0

(.=*&

Treated gr#/0

(.=*&

L 1 s i . g a " t i 2 i t 1

( 3

*%%4*

+%4*

Figure 1*Increasing ) lysing acti1ity o+ ? healthy male subjects gi1en ginger drink**#lood was

withdrawn +or lymphocyte isolation at be+ore treatment 2%3 and a+ter treatment 2#3 +rom control

and treated groups* inger drink was gi1en +or >H days e1ery a+ternoon and the cells were

incubated with target cells which had been treated with &>-thymidine with the ratio o+ HH: or

H:* Data was e6pressed as count per minute o+ the unlysed target cells*

%

#


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%

+

*%

*+

&%

&+

% ,%Da$s o% treatment

MDA #5

treated

s/67e"ts

(/m#$8LMDA #5

"#.tr#$

s/67e"ts

(/m#$8L9itami. E #5

treated

s/67e"ts

(/m#$8L9itami. E #5

"#.tr#$

Figure ?* D% and 1itamin E content in plasma o+ subjects be+ore 2H day3 and a+ter recei1ing ginger

drink +or >H days 2n@?3* 4he subjects were healthy male students li1ing in the same dormitory* D%

was analyed using the method o+ Conti et al 2KK3*9itamin E was analyed using spectro+luorometer*


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*KG ?*HK >*G >*KG * *K =* G*KH * H*?>

Retention tie (in& Retention tie (in&

Retention tie (in&

A *

6Figure"'56 croatogra of ginger oleoresin

(A&, of plasa fro control su8


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CONCLUSSON

! n human study" using ma#e adu#t hea#thy sub$e%ts" there is im&ro'ement in ( %e##s &ro#i)eration a%ti'ities and &er%entage*! m&ro'ement on ( %e##s +as a%%om&anied by resistan%e o) #ym&ho%yte %e##s against

o,idati'e stress* (his im&ro'ement +as demonstrated by the %e##s obtained )rom thesub$e%ts drinking ginger

! Resistan%e o) the #ym&ho%ytes +as not a%%om&anied by de%rease in M-. %e##

! Beside the ro#e o) ginger %om&ounds as &rote%tant against o,idati'e stress" they a&&eared to ha'e other immunoenhan%ement a%ti'ity" notab#y in im&ro'ing N/ %e## a%ti'ity* (his

im&ro'ement in N/ %e## a%ti'ity had been obser'ed in the ma#e student studied re%ei'ingginger drink )or 30 days

! ana#ysis o) ginger substan%es in the asma re'ea# the &resen%e o) the assumed gingero#grou& in the asma o) sub$e%ts drinking ginger" higher than that o) the %ontro# grou&*

! Sin%e N/ %e##s are kno+n to ha'e s&e%i)i% a%ti'ity in #ysing mutated or in)e%ted %e##s"the resu#ts o) this resear%h has sho+n the s%ienti)i% su&&ort o) the traditiona# be#ie)s thatginger %an im&ro'e body resistan%e to %ommon %o#d*


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I!7RA!+R7 R7>I7?

* 4ang O and Eisenbrand * KK?* Chinese Drugs o+ "lant (rigin: Chemistry, "harmacology and

G* Conti , orand "C, 'e1illain ", and 'emonnier %* KK* Impro1ed +luorometric determination o+

malonaldehyde* 0 Clin Chem, >G.G: ?G>-?G

* Frit )', elson 4', $ui-9elasco 9, and ercurio !D* KK* %cute intramuscular injection o+ oils or oleic acid

component protects mice against paraBuat lethality* 0 utr* K: ?-?

K* eydani !, Ou D* !antos ! and &ayek * KK* %ntio6idants and immune response in aged persons:

(1er1iew o+ present e1idence* %m 0 Clin utr* =?: =!-G!

H* 5akaria-$ungkat, F*, urahman, "rangdimurti, E*, 4ejasari* ?HH>* %ntio6idant and Immunoenhancement

%cti1ities o+ inger 2 Zingiber officinale $oscoe3 E6tracts and Compounds in In 9itro and In 9i1o ouseand &uman !ystem* utraceuticals and Foods*Q K=-H

A6K)2?7D77)!

4his reseach was +unded by the


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%)4IFI4%!

%4I )%)E$ E' CIC%< &I0%<

(Cyclea barbata L.Miers)

Fransiska RungkatZakaria,

!ndang "rangdimurti, !dna #nanta, #lbertus $eno "andoyo

&E%'4&L DE!!E$4

%)%% "EC


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"ED%&


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In+ormasi ilmiah mengenai khasiat gel cincau hijau akan mendukung

perkembangan produk pangan +ungsional yang berbentuk 7healthy dessert8,

yang akhir-akhir ini amat digemari karena dapat mengimbangi konsumsi

diet mengandung lemak dan protein hewani tinggi dan kadar serat rendah*

)anker merupakan penyakit yang diawali dari mutasi gen seluler, yang

diketahui sebagian besar disebabkan oleh +aktor e6ternal seperti polutan

kimia baik pada makanan maupun lingkungan, 1irus, dan radiasi*

ekanisme pencegahan mutasi gen dapat terjadi melalui akti+itas

antio6idan, perbaikan imunitas, atau sitotoksik 2OC$FP%IC$, KKGQ

5akaria-$ungkat et al*, ?HH3

E4(D% "EE'I4I%


60/86

E4(D% "EE'I4I%

E6traksi air daun segar dengan aBuades dilakukan secara manual karena

terbentuk gel yang kemudian didinginkan untuk memperoleh cairan sineresis*

E6trak air daun, akar dan batang dikeringbekukan*

E6trak etanol dan he6an dilakukan pada daun, akar dan batang keringbeku*

E6trak di tambahkan pada media kultur sel-sel kanker )=? 2turunan sel

leukimia3 dan &ela 2turunan sel kanker ser1i63 dan pada kultur sel lim+osit

yang diisolasi dari darah tepi seorang mahasiswa sehat, dengan tingkat

konsentrasi: C 2.? C?3Q C? 2setara dengan konsumsi segelas minuman

cincau3, C> 2?6C?3Q C 26C?3Q C 26C?3*


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&'(

(

'(

)((

c) c* c+ c, c'

-onsentrasi

. P e n g h a m b a t a n

/id

etd

hed

aib

etb

heb

aia

eta

hea

ambar * E+ek sitotoksik e6trak air 2ai3, etanol 2et3 dan he6an

2he3 dari daun 2d3, batang 2b3 dan akar 2a3 tanaman cincau hijau

terhadap pertumbuhan sel leukemia 2)=?3* E6trak air akarmempunyai persentase penghambatan diatas =M, air batang

diatas >HM, sedang air daun diatas >GM setelah konsentrasi diatas

C>* "eningkatan konsentrasi N C> 2?6 ? gelas muniman gel

cincau3 tidak nyata menaikkan akti+itas sitotoksik*


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&+(

&*(

&)(

(

)(

*(

+(

,(

'(

c) c* c+ c, c'

-onsentrasi

.

P e n g h a m b a

t a n

/id

etd

hedaib

etb

heb

aia

eta

hea

ambar ?* %kti+itas sitotoksik e6trak air 2ai3, etanol 2et3 dan he6an2he3 dari daun 2d3, batang 2b3 dan akar 2a3 tanaman cincau hijau

terhadap kehidupan sel kanker ser1i6 2&ela3*Ekstrak %ia hingga

konsentrasi C 2setara gelas minuman gel cincau3 menghambat

pertumbuhan sebesar >M, e6trak aid ??M pada konsentrasi C*

4abel %kti+itas sitotoksik 2persen3 e6trak gel cincau pada sel lim+osit manusia


63/86

#a-

pel

61 6$ 6% 6@ 6=

%id -K -? -G - -

Etd -H H -?= R G

&ed K R = >R > R

%ib - -K R ->K R ->Etb -? -? -G -? R

&eb -?? R - -? -G K

%ia > - -G -> R -? R

Eta -K ? -?= R H&ea > - = - G

2R3 menunjukkan perbedaan nyata terhadap kontrol pada selang kepercayaan KM

#ilangan negati+ menunjukkan penurunan nilai absorbansi*

4abel * %kti+itas sitotoksik 2persen3 e6trak gel cincau pada sel lim+osit manusia

yang dikultur dalam media $"I =H lengkap* !el hidup dianalisa dengan 44

4abel ? )adar alkaloid e6trak cincau %nalisa dilakukan dengan


64/86

#a-

pel

5elar

ut

& #copolain & !ropic acid

B 8"8 B 8"k B 8"8 B 8"k

Daun

%ir - - *? >*

Etanol - - H*= H*K&e6an H*? H*>G - -

#ata

ng

%ir H*=K * - -

Etanol - - H* *

&e6an H*HG H* - -

%kar

%ir - - *?> ?*?

Etanol H*>H H*= - -

&e6an - - H*K *?H

4abel ?* )adar alkaloid e6trak cincau* %nalisa dilakukan dengan

&"'C dengan menggunakan asam scopolamin-r dan tropic

sebagai standard*

)E!I"


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)E!I"


66/86

#ogor %gricultural


67/86

I)!R2D+6!I2)

!uour gro:t9o3idative stress, iunological disorder, alnutrition

"otential role +or o6idati1e-induced injury in the cancer process

speci+ically during the promotion stage 2)launig et al, KK3

!ources o+ $(! :

+rom in+lammatory cells 2Cerutti and 4rump, KK3

A need in researc to find suita8le food for cancer patients

reen gel leaf Cyclea barbata "ierscoon refresing drink in #out 7ast Asian countries

kno:n traditionally to ave cooling and anti

inflaation effects in te 8ody


68/86

!is gel /e3tracts of te leaf ad 8een reported to

ave 9

cytoto3ic activity on cancer cell linesnon to3ic on noral uan lypocyte cells

(Zakaria-Rungkat et al, $;;1&

In tis study, te leaves :ere processed to produce po:der

products

:ic included all parts and fi8res in te leaves


69/86

C>& ice n@

Fed :it po:der for

@-:k (1$"1g/kg&

!uor transplantation

!uor latent

period (days&

!uor volue

(oving pen,

!a


70/86

!pleen

#pleen cells

Resistance to

'$2$ (!!&

! cell

(Rosette&

In vitro cytoto3ic to:ard tuor cell

!uor cells


71/86

I>7R

6A!AA#7

6oloetri, #ina 1C$

#2D

(Adenocroe asay

isra" '"5"

#'-5E

(5aglia and >alentine,1C

!otal lutation

D!)*, 7llan" 1G$

DA

(!*A, *uege andAust&


72/86

&ealthy tumor tissue ecrotic tumor tissue


73/86

Figure* 'atent period o+ tumor growth in mice +ed lea+ powder

then implemented with tumor cells 2'"J43 and in miceimplemented with tumor 243* 4here is no di++erence in the latent

periodQ the lea+ did not pre1ent tumor growth in mice

%

%:+

*

*:+

&&:+

,

,:+

LP ; T T S-

L a t e n t p e r i o d " d

a $ s


74/86

%

%:+

*

*:+

&

&:+

0m+

LP ; T T S-

Figure* 4umor 1olume in mice +ed lea+ powder thenimplemented with tumor cells 2'"J43 and in mice

implemented with tumor 243* 4he lea+ powder decrease

tumor 1olume in mice


75/86

%

&

'

G

r

a

m

s LP ; T T S-

Ne"r#se


76/86

%

*%

&%

,%'%

+%

. necrotic

tissue

LP ; T T S-

Figure* "ercentage o+ necrotic tumor tissue in mice +ed

lea+ powder then implemented with tumor cells 2'"J43 and

in mice implemented with tumor 243* 4he lea+ powder

increase the percentage o+ tumor necrotic tissue in mice

! 0ell" .


77/86

Figure* "ercentage o+ 4 cells by rosette techniBue in mice +ed lea+ powder

then implemented with tumor cells 2C"3, implemented with tumor without

the lea+ 243 and in standard control mice 2!C3* Feeding lea+ powder

increased 4cell inspite o+ the tumor growth* 4umor implementations

without the lea+ markedly reduced 4 cell percentage

*(=&

>=+ )=)

-P T S-


78/86

+?,

?,>>

%

*%

&%

,%

'%

+%

%

>%

)%

D

e

a

d

t

u

m

o

r

c

e

l

l

s

"

.

S- T LP;T

Figure* Cytoto6icity o+ 4 cells +rom mice +ed lea+ powder thentransplanted with tumor cells 2'"J 43, with tumor alone and

standard diet 2!C3* !light increase in cytoto6icity in 4 and '"J4,

indicate 4 cell recognition o+ the tumor antigen


79/86

%%%

%+%

*%%

*+%

&%%

&+%

,%%

,+%

'%%

'+%

to weeks markedly decrease cells; resistance to

o6idati1e stress


80/86

MDA

%

+

*%

*+

LP ; T T S-

SOD

%

*%%

&%%

,%%

'%%

+%%

LP ; T T S-

Figure* D% content and !(D acti1ity in mice +ed lea+

powder then implemented with tumor cells 2'"J43Q in mice

implemented with tumor 243 and in mice +ed standard diet*

4he lea+ powder increase !(D acti1ity in mice but reduceD% in the li1er


81/86

%

&%%

'%%

(%%

-ata$ase GSH!P@ GSH

LP ; T T S-

Figure* !& content and antio6idant enyme acti1ity in mice +edlea+ powder then implemented with tumor cells 2'"J43Q in mice

implemented with tumor 243 and in mice +ed standard diet* 4he

lea+ powder reduce li1er catalase acti1ity but did not e++ect the

other enyme acti1ity in the li1er*

C(C'


82/86

C(C'

* 4here is no di++erence in the latent period o+ the tumor growth in

mice* 4he lea+ powder decrease tumor 1olume and tumor mass butincrease tumor necrotic tissue up to > times

?* Feeding lea+ powder increased 4cell inspite o+ the tumor growth*

!light increase in cytoto6icity in 4 and '"J4, indicate 4 cell

recognition o+ the tumor antigen

"rolonged tumor growth +rom > to weeks markedly decrease

cells; resistance to o6idati1e stress

>* 4he lea+ powder increase !(D acti1ity in mice but reduce

D% in the li1er $educe li1er catalase acti1ity but did not

e++ect the other enyme acti1ity in the li1er*


83/86

Lang sudah.sedang kami teliti:

• )ayu manis, cacao

• )umis kucing

• )ecombrang

• 'engkuas, minyak buah merah, Dll

%spek: imunomodulator, anti kanker

anti obesity (baru)

C l i


84/86

Conclusion

•"otensi komoditi lokal sangat besar untuk produk pangan +ungsional

• #ahan baku pangan tradisional mengandungkomponen +ungsional.bioakti+

• )etersediaan bahan berlimpah• "otensi untuk suplemen dari bahan baku pangan,

rempah-rempah, tanaman obat

• $ekayasa pangan +ungsional dari bahan.reseptradisional

• "otensi menu diet tradisional

• asih banyak komoditi yang harus diteliti

T+ 0%dministration #uilding

#ogor agricultural


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T+*(0

1o2

g g y

Indonesia


86/86

2008 kuliah8 indigenous

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