biotechnology training 2012

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Training on

Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA Barcoding

Organized by

Dr.Prem P Jauhar Laboratories of Molecular Sciences

New Delhi India-110092

1st June 2012 to 30th June 2012

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BACKGROUND

Realizing the importance of forensic molecular biology, DNA fingerprinting, DNA barcoding, and DNA diagnostics in human health care, molecular breeding, functional biomedical genetics, drug development and gene therapy with application of evolutionary studies and the genetic engineering. The Dr.Prem P Jauhar Laboratories of Molecular Sciences, New Delhi India is take initiative at international level organizing Training On Forensic Molecular Biology, Biotechnology, DNA Fingerprinting And DNA Barcoding 2012. The main aim of this training is to provide hands on experience to the various prospects of this new emerging field and share opportunities among scientists, engineers, doctors, research scholars and students in India and abroad. The training will provide an excellent platform for work and share them with science, engineering, molecular biology, forensic, evolutionary biology, molecular breeding, genetically modified crop/organism/animal (GMOs, LMOs and transgenic) and medical background and help to come together, take the challenges in rapid development of this emerging field across the globe toward the biodiversity biotechnology, biosystematics, livestock genetic diversity, marine biodiversity and biotechnology, marine and fresh water fish biodiversity, ornithological studies, fisheries biotechnology, insects and pests diversity, agro entomology, integrated biological control ,sericulture biotechnology, animal medicine, intellectual property rights and patents, animal and veterinary products, animal nutrition and feed technology, conservation strategies and anthropological molecular survey studies.

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Training Theme

1. General Laboratory Experiment : (Hands on): DNA isolation from Plant, Bacteria, Yeast, Fungi , Animal Tissue (Heart, Kidney, Lever, and Blood etc), Quantification and Quality check of isolated DNA, DNA Dilution, PCR Amplification of DNA using Multiplex Primers, PCR Amplification of DNA using random 10mer primers for RAPD, PCR Amplification of DNA using SSR primers, Micro Satellite Analysis and STR Primer for DNA Fingerprinting, Separation Following Automated electrophoresis, Data Capturing, Data Analysis and DNA fingerprint development.

2. Human DNA Fingerprinting and Identification :Laboratory Experiments (Hands on): DNA isolation from human blood, quantification and quality check of isolated DNA, DNA dilution, PCR amplification of DNA using multiplex primers, fragment (allele) separation following electrophoresis, data capturing, data analysis and DNA fingerprint development

3. DNA Fingerprinting of Plants following Random Amplified Polymorphic DNA (RAPD) and Microsatellite techniques: Laboratory Experiments (Hands on): Isolation of DNA, quantification and quality check of isolated DNA, DNA dilution, PCR amplification of DNA using random 10‐ mer primers for RAPD, PCR amplification of DNA using SSR/ISSR/STR primers for microsatellite analysis, agarose gel electrophoresis of PCR products for RAPD, allele separation following automated capillary electrophoresis for microsatellite analysis, data collection and data analysis

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4. DNA barcoding of animals using Cytochrome C Oxidase subunit I (COI):Laboratory experiments (Hands on): DNA isolation, quantification and quality check of the isolated DNA, DNA dilution, PCR amplification of DNA using COI primers, agarose gel electrophoresis of PCR products, gel elution of PCR products, sequencing of PCR products and sequence data analysis.

5. Forensic Molecular Biology: DNA Fingerprinting method of plant, animals, microorganisms, human and Diagnostics (Disease Diagnostics, Heritable Disease Diagnostics, Cancer Diagnostics, Pathogen Diagnostics, Parental Diagnostic, and Mass Disaster of Natural Calamities and Army Causality.

6. DNA Fingerprinting: Methods and protocols used in DNA Fingerprinting of Human, DNA Fingerprinting of animals, DNA Fingerprinting of Fishes, DNA Fingerprinting of plant, DNA Fingerprinting of fungi, Bacteria, and Yeast etc. Implication and issues with techniques.

7. DNA Bar-coding: Methods used in Human DNA Barcoding, Animal DNA Barcoding, Insect DNA Barcoding, Fish DNA Barcoding and microorganism DNA Barcoding, Plant DNA Barcoding, Implication and issues with techniques etc.

8. Phylogenic Analysis: Molecular Phylogenic Analysis, Genotyping of Plant, Animal, Fish, Insect and Microbial Population, Individual and Community, Seed Purity, Genetic Containment, Germplasm Characterization, Allele and Gene Discovery, Implication and issues with technique etc.

9. Genetic Engineering: Gene Isolation, Gene Detection, GMOs, LMOs Detection, GMO Food Detection, Microbial Contamination Determination.

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10. Molecular Marker Technology: SSR, ISSR, RAPD, STR, Molecular Marker Assisted Selection, Molecular Breeding, Cultivar Development, Molecular Mapping, Gene Mapping, Genetic Structure Analysis, Molecular Marker Development.

11. Biotechnology: Nucleic Acid Extraction, Purification, Dilution, Spectrophotometer Quantization, electrophoresis, DNA Elution, PCR, PCR Product Purification, Genetic (DNA) Fingerprint, DNA Molecular Profile Development.

12. DNA Fingerprinting Software and Biostatistics Analysis: DNA Fingerprinting Profile Regeneration, DNA Barcoding, Diversity Matrix Development, Phylogenetic Tree Development, Allele Frequency, Private Allele, Banding Pattern, Heterozygosis, Primer discriminatory Power Test, and molecular Biostatistics analysis.

13. Result Analysis and Interpretation: on Forensic Molecular Biology, Molecular genetics, Evolutionary aspects and Phylogenetic background.

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IMPORTANT DATES AND INFORMATION First Batch

Application Deadline 15 th May, 2012 Acceptance of Application 15 th May, 2012 Acceptance of Application for Abroad candidate with fee submission 25th May 2012

Second Batch Application Deadline 25th June, 2012 Acceptance of Application for Second Batch 25 th June 2012. All fee will be accepted through bank transfer only

Confirmation: Please allow up to 5 days for mailed confirmation of your registration. Cancellation and Refund: Member wishing to cancel their participation should inform JLMS Office in writing

Before 15th May 2012 for first batch and 25June 2012 for second batch After that date there will be no refund of the registration fee. Office receives cancellations before 15 May 2012for first batch And 25th June for second batch, the total training fee will be refunded, less Rs. 1500 or $50. Candidate register after 25th May 2012 for first batch and 25th June for second batch will not claim for refund in any case. If you cannot attend, you may send a substitute person. The original registrant must submit a written authorization for such a change. Please note that refunds will only be made after the training program. After joining the training program there will be no refund of registration fee.

REGISTRATION FEES First Batch 1st June to 30th June 2012 Second Batch 1st July to 30th July 2012 Before 15th May, 2012 Foreign Indian Before 15th May, 2012 Foreign Indian Govt. Faculty/Scientist 2000 USD 18000 INR Govt. Faculty/Scientist 2000 USD 18000 INR Private Professionals/ NGO 2000 USD 18000 INR Private Professionals/ NGO 2000 USD 18000 INR Student 1000 USD 11000 INR Student 1000 USD 11000 INR After 15th May, 2012 Foreign Indian After 25th June, 2012 Foreign Indian Govt. Faculty/Scientist 2050 USD 18500 INR Govt. Faculty/Scientist 2050 USD 18500 INR Private Professionals/ NGO 2050 USD 18500 INR Private Professionals/ NGO 2050 USD 18500 INR Student 1025 USD 11300 INR Student 1025 USD 11300 INR

*Registration Fees is inclusive of Training Kit, Abstract Book, Proceedings

Candidate need to deposit their all fee though Bank only with detail given to registration form and /or confirmation letter

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Registration form

“Training on Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA Barcoding” Dr.Prem P Jauhar Laboratories of Molecular Sciences

Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092

Full Name: ………………………………………………………………………………………………………………………………………………… Designation: …………………………………………………………………………… ………………………………… ……………………………… Institution: …………………………………………………………………… ………………………………… ………………………………… ……… Address with Pin Code: ………………………………………………… ………………………………… ……………………………………………… ………………………………… ………………………………… ………………………………… ……………………………………………… …… a) Official: ……………………………………………………………………………… ………………………………… ………………………………… …………………………………………………………………………… ………………………………… ………………………………………………… b) Residence: ………………………………………… ………………………………… ………………………………… ………………………………… ………………………………………………………………………………………………………………………………………………………………… Cell Phone: ………………………… Fax: ……………………………E-Mail: ……………………………………Telephone with area Code: ……………… Registration Fees paid details: INR/USD…………………………………………… Bank Transfer with date: ………………………………………………… …… Accommodation on Request only : Hotel A/C / Non A/C /PG

Bank Detail: - Punjab National Bank- A/C -2972001500001162 (IFSC CODE - PUNB0297200. MICR CODE: Branch CODE: 297200.) Name of Beneficiary -Vinay Kumar Singh

E-Mail the Registration form to : Vinay Kumar Singh Director Training and Projects, Dr.Prem P Jauhar Laboratories of Molecular Sciences Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092 Contacts- 91+9560757331, 8802065581, 9899242448, 9716159717, 7830959033 [email protected] [email protected] Login: www.jauharlabs.com

Signature Date: Place:

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Training on Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA

Barcoding Dr.Prem P Jauhar Laboratories of Molecular Sciences

Program Schedule: - June 1st to June 30th for First Batch. 1st June Friday-

@ 2.00 to 2.30 Pm- Welcome By Director Dr. Prem P. Jauhar Laboratories of Molecular Sciences @ 2.30 to -3.00 Pm – Address to Training Objectives and Needs @ 3.00 to 4.00 Pm- Introduction with Staff and Trainees @ 4.00 to 4.30 Pm - Tea Party. 2nd June Saturday

@8.30 to 10.30 Am-. Methods of Nucleic Acid Isolation and Purification @10.30 to 2.00 Pm- Isolation of Nucleic Acid from Plant, Fungi, Bacteria, Cyanobacteria, and Algae Etc. 3rd June Sunday

@8.30 to 10.30 Am- Isolation 0f Nucleic Acid from Animal Tissue, Heart, Leaver, Kidney, Hair and Blood Etc. @10.30 to 12.30 Pm- Electrophoresis 0f DNA /RNA, Qualitative And Quantitative Analysis of Nucleic Acid. @12.30 to 2.0 Pm- Purification of Nucleic Acid for RAPD, ISSR, SSR, STR, and General PCR.

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4th June Monday @8.30 to 10.30Am - Dilution of Nucleic Acid for RAPD, ISSR, SSR, STR, General PCR and diagnostics PCR @10.30 to 12.30 Pm- Running of RAPD PCR with Plant, Bacteria and Fungi DNA @12.30 to 2.0 Pm- Lecture -Method of RAPD Analysis Result Interpretation and Application 5th June Tuesday

@8.30 to 10.30 – Electrophoresis of RAPD PCR Product and Result Analysis @ 10.30 to 12.30 Pm- Running of SSR PCR With Plant, Bacteria, Fungi and /Or Fish/Or Insects DNA. @12.30 to 2.0 Pm- Lecture -Method of SSR Analysis Result Interpretation and Application 6th June Wednesday

@8.30 to 10.30 – Electrophoresis of SSR PCR Product and Result Analysis @10.30 to 12.30 Pm-. Running of ISSR PCR with Plant, Bacteria, Fungi and /Or Fish/Insects DNA @12.30 to 2.0 Pm- Lecture -Method of ISSR Analysis, Result Interpretation and Application 7th June Thursday

@8.30 to 10.30Am – Electrophoresis of ISSR PCR Product Result and Analysis @ 10.30 to 12.30 Pm- Running of STR PCR With Plant, Bacteria, Fungi and /Or Fish And /Or Insect DNA. @12.30 to 2.0 Pm- Lecture -Method of STR Analysis Result Interpretation and Application 8th June Friday

@8.30 To 10.30Am – Electrophoresis of STR PCR Product Result Analysis @ 10.30 To 12.30 Pm- Lecture –Advance Techniques of Genotyping, Problem and Solution

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@12.30 To 2.0 Pm- Quiz and Round Table Discussion on SSR, ISSR, RAPD, STR, AFLP, RFLP, and Other Genotyping Techniques. 9th June Saturday

@8.30 To 10.30Am – Primer Designing for RAPD, ISSR, SSR, STR, DNA Barcoding, Gene, GMO, LMO, and Cancer Diagnostics, @ 10.30 To 12.30 Pm- Primer Designing for Inheritable Disease Diagnostics and Pathogen Diagnostics @12.30 To 2.0 Pm- Lecture on Tools and Software for Molecular Biologists. @2.0 To 5.0 Pm- Lecture –Power Point Presentations from Trainee on Molecular Marker Techniques 10th June Sunday Closed

11th June Monday

@8.30 to 10.30Am –

DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects Through Random Amplified Polymorphic DNA (RAPD)

@ 10.30 to 12.30 Pm-

Case Studies on DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects Through Random Amplified Polymorphic DNA (RAPD)

@12.30 to 2.0 Pm- Exercise with Software and Analysis Methods 12th June Tuesday

@8.30 to 10.30Am – DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects Through Simple Sequence Repeats(SSR)

@ 10.30 to 12.30 Pm- Case Studies On DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects in Simple Sequence Repeats

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(SSR) Studies @12.30 to 5.0 Pm- Lecture –Power Point Presentations from Trainee on RAPD and SSR Application and Issues 13th June Wednesday

@ 08.30 to 10.30Am - DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects through ISSR. @ 10.30 to 12.30 Pm- Case Studies On DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects through in ISSR Studies @12.30 to 02.00 Pm- Lectures – Comparative Analysis of RAPD, SSR, ISSR Techniques 14th June Thursday

@ 08.30 to 10.30Am - DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects through STR @ 10.30 to 12.30 Pm- Case Studies on DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects in STR Studies @12.30 to 02.00 Pm- Software and Analysis Methods 15th June Friday.

@ 08.30 to 10.30 Pm- GMO/LMO/Diagnostics from marketed product, and field’s crops, @ 10.30 to 12.30 Pm- Exercise on PCR Program for RAPD, ISSR, SSR, STR, Etc. @12.30 to 02.00 Pm- Lecture –PCR Optimization Exercise for Better Amplification in Molecular Marker Analysis 16th June Saturday

@ 08.30 to 10.30 – Webs Based Online Software Based Data Analysis Exercises @ 10.30 to 12.30 Pm- PC Based Software for Data Analysis Exercise @12.30 to 02.00 Pm- Lecture –Advances High Throughputs Techniques for Genotyping and Useful Database 17th June Sunday Closed

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18th June Monday

@ 08.30 to 10.30 Am– Exercises on Gene Isolation, Gene Detection, GMOs, LMOs Detection, GMO Food Detection, Microbial Contamination Determination.

@ 10.30 to 12.30 Pm- Molecular Phylogenic Analysis, Genotyping of Plant, Animal, Microbial Population, Individual and Community, Seed Purity, Genetic Containment, Germplasm Characterization, Allele and Gene Discovery Etc.

@12.30 to 02.00 Pm- Lecture –Diversity Matrix Development, Phylogenetic Tree Development, Allele Frequency, Private Allele, Banding Pattern, Heterozygosis, Primer Discriminatory Power Test

19th June Tuesday

@ 08.30 To 10.30 Am – DNA Elution from Agrose Gel, PCR Product Purification, @ 10.30 To 02.00 Pm-. Exercise on Molecular Marker Assisted Selection, Molecular Breeding, Cultivar Development, Molecular Mapping

Exercise – Data Interpretation, Identification of Linked Gene Or Linked Traits, Confirmation Of Gene Transfer in Next Generation, Molecular Marker Development, Banding Pattern And Its Application

20th June Wednesday

@ 08.30 to 10.30 Am – Exercises on 16S RNA Based Molecular Phylogenetic Analysis Protocol and Methods of Molecular Phylogenic Testing With Plant, Bacteria, Fungi , Fish/Or Insect DNA.

@ 10.30 to 02.00 Pm - 21st June Thursday

@08.30 to 0 9.30 Am- Exercises on – 16S RNA Based Molecular Phylogeny Of 10 Bacterial Species @ 09.30 to 10.00 Am- Exercises on -16S RNA Based Molecular Phylogeny 10 Fungi Species @10.00 to 10.30 Am- Exercises on -16S RNA Based Molecular Phylogeny 10 Yeast Species @10.30 to 11.00 Am Exercises on - 16S RNA Based Molecular Phylogeny 10 Cyanobacteria Species

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@11.00 to 01.00 Pm Exercises on -16S RNA Based Molecular Phylogeny 10 Actinomycetes Species 22nd June Friday

@08.30 to 10.30 Am-, Exercises on – Phylogenic Dendrogram, Type of Dendrogram Interpretation of Dendrogram, Bootstrap, @ 10.30 to 11.30 Am- Exercises on -Jacquard’s Standards Similarity Coefficient, Diversity Coefficient, Minimum Diversity, Maximum

Diversity, Average Diversity and Its Interpretation. Within Species and Between Species Diversity @11.30 to 01.00 Pm- Exercises on –Principal Coordinate Analysis, UPGMA, Neighbors Joining Method and Other Methods, 23rd June Saturday

@8.30 to 10.30Am – Reviews of All Exercise and Hands on Techniques @ 10.30 to 12.30 Pm-. Review of All Software and Statistical Analysis @12.30 to 5.0 Pm- Lecture /or Round Table Discussion on - Parentage, Murder, Complex Case, Twins, Family Tree, Pedigree, Mass

Casualty, Military casualty, Hybrids, Ploidy Levels, Mutants, Social Habits, Behavior Analysis Etc. 24th June Sunday Closed

25th June Monday

@08.30 to 10.30Am – PCR with Human DNA for DNA Barcoding. @ 10.30 to 12.30 Pm- Electrophoresis of PCR Product and Verification of Product @12.30 to 02.00 Pm- Lecture –Sequence Regeneration and Barcode Development 26th June Tuesday

@08.30 to 10.30 Am – PCR with Chicken DNA for DNA Barcoding. @ 10.30 to 12.30 Pm- Electrophoresis of PCR Product and Verification of Product

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@12.30 to 02.00 Pm- Lecture –Sequence Regeneration and Barcode Development 27th June Wednesday

@08.30 to 10.30 Am – PCR with Fish DNA for DNA Barcoding. @ 10.30 to 12.30 Pm- Electrophoresis of PCR Product and Verification of Product @12.30 to 02.00 Pm- Lecture –Sequence Regeneration and Barcode Development 28th June Thursday

@08.30 to 10.30 Am – PCR with Mouse DNA for DNA Barcoding. @ 10.30 to 12.30 Pm- Electrophoresis of PCR Product and Verification of Product @12.30 to 02.00 Pm- Lecture –Sequence Regeneration and Barcode Development 29th June Friday

@08.30 to 10.30 Am – Lectures on Problem and Special Issue with DNA Barcoding @ 10.30 to 12.30 Pm Round Table Discussion on DNA Barcoding @12.30 To 05.00 Pm Lecture –Power Point Presentations from Trainee on DNA Barcoding 30th June Saturday

@ 10.30to 11.30Am Vote for thanks by director Dr.Prem P.Jauhar Laboratories of Molecular Sciences @ 10.30to 12.30 Pm Certificate Distribution Program

@12.30to 01.00 Pm Tea Party

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Training on Forensic Molecular Biology, Biotechnology, DNA Fingerprinting and DNA

Barcoding Dr.Prem P Jauhar Laboratories of Molecular Sciences

Program Schedule: - July 1st to July 30th for Second Batch. 1st July Sunday-

@ 02.00 to 02.30 Pm Welcome By Director Dr. Prem P. Jauhar Laboratories of Molecular Sciences @ 02.30 to 03.00 Pm Address to Training Objectives and Needs @ 03.00 to 04.00 Pm Introduction with Staff and Trainees @ 04.00 to 04.30 Pm Tea Party. 2nd July Monday

@08.30 to 10.30 Am Methods of Nucleic Acid Isolation and Purification. @10.30 to 02.00 Pm Isolation of Nucleic Acid from Plant, Fungi, Bacteria, Cyanobacteria, and Algae Etc.

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3rd July Tuesday

@08.30 to 10.30 Am Isolation 0f Nucleic Acid from Animal Tissue, Heart, Leaver, Kidney, Hair and Blood Etc. @10.30 to 12.30 Pm Electrophoresis 0f DNA /RNA, Qualitative And Quantitative Analysis of Nucleic Acid. @12.30 to 02.00 Pm Purification of Nucleic Acid for RAPD, ISSR, SSR, STR, and General PCR. 4th July Wednesday

@08.30 to 10.30Am Dilution of Nucleic Acid for RAPD, ISSR, SSR, STR, General PCR and diagnostics PCR @10.30 to 12.30 Pm Running of RAPD PCR with Plant, Bacteria and Fungi DNA @12.30 to 02.00 Pm Lecture -Method of RAPD Analysis Result Interpretation and Application 5th July Thursday

@08.30 to 10.30 Am Electrophoresis of RAPD PCR Product and Result Analysis @10.30 to 12.30 Pm Running of SSR PCR With Plant, Bacteria, Fungi and /Or Fish/Or Insects DNA. @12.30 to 02.00Pm Lecture -Method of SSR Analysis Result Interpretation and Application 6th July Friday

@08.30 to 10.30 Am Electrophoresis of SSR PCR Product and Result Analysis @10.30 to 12.30 Pm Running of ISSR PCR with Plant, Bacteria, Fungi and /Or Fish/Insects DNA. @12.30 to 02.00 Pm Lecture -Method of ISSR Analysis, Result Interpretation and Application 7th July Saturday

@ 08.30 to 10.30 Am Electrophoresis of ISSR PCR Product Result and Analysis

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@ 10.30 to 12.30 Pm Running of STR PCR With Plant, Bacteria, Fungi and /Or Fish And /Or Insect DNA. @12.30 to 02.00 Pm Lecture -Method of STR Analysis Result Interpretation and Application 8th July Sunday Closed

9th July Monday

@ 08.30 to 10.30 Am Electrophoresis of STR PCR Product Result Analysis @ 10.30 to 12.30 Pm Lecture –Advance Techniques of Genotyping, Problem and Solution @12.30 to 02.00 Pm Quiz and Round Table Discussion on SSR, ISSR, RAPD, STR, AFLP, RFLP, and Other Genotyping Techniques. 10th July Tuesday

@ 08.30 to 10.30 Am Primer Designing for RAPD, ISSR, SSR, STR, DNA Barcoding, Gene, GMO, LMO, and Cancer Diagnostics, @ 10.30 to 12.30 Pm Primer Designing for Inheritable Disease Diagnostics and Pathogen Diagnostics @ 12.30 to 02.00 Pm Lecture on Tools and Software for Molecular Biologists. @ 02.00 to 05.00 Pm Lecture –Power Point Presentations from Trainee on Molecular Marker Techniques 11th July Wednesday

@ 08.30 to 10.30 Am DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects Through Random Amplified Polymorphic DNA (RAPD)

@ 10.30 to 12.30 Pm Case Studies on DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects Through Random Amplified Polymorphic DNA (RAPD)

@12.30 to 02.00 Pm Exercise with Software and Analysis Methods 12th July Thursday

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@ 08.30 to 10.30 Am DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects Through Simple Sequence Repeats (SSR)

@ 10.30 to 12.30 Pm Case Studies On DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects in Simple Sequence Repeats (SSR) Studies

@ 12.30 to 05.00 Pm Lecture –Power Point Presentations from Trainee on RAPD and SSR Application and Issues 13th July Friday.

@ 08.30 to 10.30 Am DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects through ISSR. @ 10.30 to 12.30 Pm Case Studies On DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects through in ISSR Studies @ 12.30 to 02.00 Pm Lectures – Comparative Analysis of RAPD, SSR, ISSR Techniques 14th July Saturday

@ 08.30 to 10.30Am DNA Fingerprinting Methods and Protocols for Plant, Animals, Fishes, Microbe and Insects through STR @ 10.30 to 12.30 Pm Case Studies on DNA Fingerprinting of Plant, Animals, Fishes, Microbe and Insects in STR Studies @12.30 to 02.00 Pm- Software and Analysis Methods 15th July Sunday Closed

16th July Monday

@ 08.30 to 10.30 Pm GMO/LMO/Diagnostics from marketed product, and field’s crops @ 10.30 to 12.30 Pm Exercise on PCR Program for RAPD, ISSR, SSR, STR, Etc @12.30 to 02.00 Pm Lecture –PCR Optimization Exercise for Better Amplification in Molecular Marker Analysis

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17th July Tuesday

@ 08.30 to 10.30 Am Webs Based Online Software Based Data Analysis Exercises @ 10.30 to 12.30 Pm PC Based Software for Data Analysis Exercise @12.30 to 02.00 Pm Lecture –Advances High Throughputs Techniques for Genotyping and Useful Database 18th July Wednesday

@ 08.30 to 10.30 Am Exercises on Gene Isolation, Gene Detection, GMOs, LMOs Detection, GMO Food Detection, Microbial Contamination Determination.

@ 10.30 to 12.30 Pm Molecular Phylogenic Analysis, Genotyping of Plant, Animal, Microbial Population, Individual and Community, Seed Purity, Genetic Containment, Germplasm Characterization, Allele and Gene Discovery Etc.

@12.30 to 02.00 Pm Lecture –Diversity Matrix Development, Phylogenetic Tree Development, Allele Frequency, Private Allele, Banding Pattern Heterozygosis, Primer Discriminatory Power Test

19th July Thursday

@ 08.30 To 10.30 Am DNA Elution from Agrose Gel, PCR Product Purification @ 10.30 To 02.00 Pm Exercise on Molecular Marker Assisted Selection, Molecular Breeding, Cultivar Development, Molecular Mapping

Exercise – Data Interpretation, Identification of Linked Gene Or Linked Traits, Confirmation Of Gene Transfer in Next Generation, Molecular Marker Development, Banding Pattern And Its Application

20th July Friday

@ 08.30 to 10.30 Am Exercises on 16S RNA Based Molecular Phylogenetic Analysis @ 10.30 to 02.00 Pm Protocol and Methods of Molecular Phylogenic Testing With Plant, Bacteria, Fungi , Fish/Or Insect DNA.

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21st July Saturday

@08.30 to 0 9.30 Am Exercises on – 16S RNA Based Molecular Phylogeny Of 10 Bacterial Species @ 09.30 to 10.00 Am Exercises on -16S RNA Based Molecular Phylogeny 10 Fungi Species @10.00 to 10.30 Am Exercises on -16S RNA Based Molecular Phylogeny 10 Yeast Species @10.30 to 11.00 Am Exercises on - 16S RNA Based Molecular Phylogeny 10 Cyanobacteria Species @11.00 to 02.00 Pm Exercises on -16S RNA Based Molecular Phylogeny 10 Actinomycetes Species 22nd July Sunday Closed

23rd July Monday

@ 08.30 to 10.30 Am Exercises on – Phylogenic Dendrogram, Type of Dendrogram, Interpretation of Dendrogram, Bootstrap @ 10.30 to 11.30 Am Exercises on -Jacquard’s Standards Similarity Coefficient, Diversity Coefficient, Minimum Diversity, Maximum

Diversity, Average Diversity and Its Interpretation. Within Species and Between Species Diversity @11.30 to 01.00 Pm Exercises on –Principal Coordinate Analysis, UPGMA, Neighbors Joining Method and Other Methods, 24th July Tuesday

@ 8.30 to 10.30 Am Reviews of All Exercise and Hands on Techniques @ 10.30 to 12.30 Pm Review of All Software and Statistical Analysis @ 12.30 to 05.00 Pm Lecture /or Round Table Discussion on - Parentage, Murder, Complex Case, Twins, Family Tree, Pedigree, Mass

Casualty, Military casualty, Hybrids, Ploidy Levels, Mutants, Social Habits, Behavior Analysis Etc 25th July Wednesday

@08.30 to 10.30 Am PCR with Human DNA for DNA Barcoding

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@ 10.30 to 12.30 Pm Electrophoresis of PCR Product and Verification of Product @12.30 to 02.00 Pm Lecture –Sequence Regeneration and Barcode Development 26th July Thursday

@08.30 to 10.30 Am PCR with Chicken DNA for DNA Barcoding. @ 10.30 to 12.30 Pm Electrophoresis of PCR Product and Verification of Product @12.30 to 02.00 Pm Lecture –Sequence Regeneration and Barcode Development 27th July Friday

@ 08.30 to 10.30 Am PCR with Fish DNA for DNA Barcoding. @ 10.30 to 12.30 Pm Electrophoresis of PCR Product and Verification of Product @ 12.30 to 02.00 Pm Lecture –Sequence Regeneration and Barcode Development 28th July Saturday

@ 08.30 to 10.30 Am PCR with Mouse DNA for DNA Barcoding. @ 10.30 to 12.30 Pm Electrophoresis of PCR Product and Verification of Product @ 12.30 to 02.00 Pm Lecture –Sequence Regeneration and Barcode Development 29th July Sunday

@ 08.30 to 10.30 Am Lectures on Problem and Special Issue with DNA Barcoding @ 10.30 to 12.30 Pm Round Table Discussion on DNA Barcoding @ 12.30 To 05.00 Pm Lecture –Power Point Presentations from Trainee on DNA Barcoding

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30th July Monday

@ 10.30to 11.30Am Vote for thanks by director Dr.Prem P.Jauhar Laboratories of Molecular Sciences @ 10.30to 12.30 Pm Certificate Distribution Program @ 12.30to 01.00 Pm Tea Party Note

***Above daily program is part of experimentation, discussion, exercise, hands on, *** Some protocols, experiment, exercise can be additionally include at time in our work. *** Program will be review weekly and would be modified as per needs

Dr.Prem P Jauhar Laboratories of Molecular Sciences Patparganj Industrial Area near Anand Vihar ISBT New Delhi India 110092

Contacts-8802065581, 9899242448, 9716159717, 7830959033 [email protected] [email protected]

Login: www.jauharlabs.com

biotechnology training 2012

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