biology lecture - photosynthesis
TRANSCRIPT
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Photosynthesis
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Photosynthesis
• Process by which plants, algae, and some microorganisms use solar energy and CO2 and convert it into chemical energy
• Endergonic• Redox reaction• Done by autotrophs
• Glucose used for: fuel own plant respiration (50%), growth, make other important compounds (amino acids, cellulose, starch, sucrose)
6CO2 + 6H2O C6H12O6 + 6O2
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Photosynthesis is a Redox Reaction
Cellular Respiration
Photosynthesis
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Atmospheric Oxygen
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Light
• Light is the source of energy for photosynthesis
– Made of photons – packets of kinetic energy
– Part of electromagnetic spectrum
– 3 types from the sun get to the earth
• Ultraviolet
• Visible
• Infrared
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The Electromagnetic Spectrumand Visible Light
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Pigments
• Pigment – Substance that absorbs light energy
• Several types of pigments:
– Chlorophyll a – most abundant, green pigment, absorb blue/red, reflect green
– Accessory Pigments:
• Chlorophyll b – absorb blue/red, reflect green
• Carotenes – absorb blue, reflect orange/red
• Xanthophylls - absorb purple/blue/ green, reflect yellow
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Pigments
Pigment Color Organisms
Major PigmentChlorophyll a
green (or yellow) plants, algae, bacteria
Accessory PigmentChlorophyll b
yellow plants, algae
Carotenoids (xanthophylls and
carotenes)
orange, red, yellow plants, algae, bacteria, archaea
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Absorption Spectrum of Pigments
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Chloroplasts
• Stroma – inner fluid with DNA, ribosomes, fluid• Grana – Stacks of thylakoid• Thylakoid – Disks, membranes with
photosynthetic pigments• Photosystem – in thylakoid membrane, absorbs
light and converts it to usable energy– Chlorophyll a (approx. 300 molecules)
• Reaction Center
– Accessory pigments (approx. 50 molecules)• Antenna pigment to funnel light to reaction center
– Proteins
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Chloroplast
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Chloroplasts
• Mainly found in cells in the LEAF
– Lots of surface area to absorb light
– Has abundant water
– Main site of gas exchange
• Exchange occurs through stomata surrounded by guard cells
– Most abundant in mesophyll
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Structure of a leaf
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Photosynthesis Overview
• Happens in 2 stages
– Light Reactions – convert solar energy into chemical energy
• Occurs in thylakoid membrane
– Carbon Reactions/Calvin Cycle – use ATP and NADPH to reduce CO2 to glucose
• Occurs in the stroma
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Photosynthesis Overview
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The Light Reactions
• Photosystem II –– Pigment molecules absorb light and transfer to
reaction center (chlorophyll a)
– Water is split into 2H+ and ½ O2
– Water donates 2 electrons• Energy “excites” 2 electrons to a higher energy orbital
• Chlorophyll a ejects “excited” electrons to first electron transport chain (ETC) passes to Photosystem I
– ETC makes a proton gradient from stroma into the thylakoid space• ATP synthase uses proton gradient to make ATP
(chemiosmotic phosphorylation)
• Used in carbon reactions
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Light Reactions: Photosystems
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The Light Reactions
• Photosystem I –– Pigment molecules absorb light and transfer to
reaction center (chlorophyll a)
– 2 electrons come from first ETC (Photosystem II)
– Energy “excites” 2 electrons to a higher energy orbital
– Chlorophyll a ejects “excited” electrons to first electron transport chain (ETC)
– Electrons are passed to NADP+ to reduce it to NADPH (used in carbon reactions)
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ATP Generation – Photosystem I
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The Light Reactions
• Final Outputs of Light Reactions
– Oxygen (emitted from plant)
– NADPH (used in Calvin Cycle)
– ATP (used in Calvin Cycle)
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The Calvin Cycle
• Also known as: Carbon reactions, Dark reactions• Occurs in the stroma• Uses ATP and NADPH to make glucose from CO2
• Calvin Cycle:– Step 1: Carbon fixation – incorporation of CO2 into an
organic molecule• CO2 combines with RuBP, using enzyme called rubisco• Makes PGAL (2 3 carbon molecules)
– Step 2: Reduction of PGAL from step 1 to G3P• Uses NADPH and ATP from light reactions
– Step 3: G3P converted glucose– Step 4: Regeneration of RuBP
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The Calvin Cycle
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The Calvin Cycle
• Calvin Cycle must “turn” 6 times to make 1 glucose molecule– 1 turn for each CO2 fixed
– Calvin Cycle Uses:• 12 ATP
• 12 NADPH
• Another 6 ATP to regenerate RuBP
• Final Outputs of Calvin Cycle– C6H12O6
– O2
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C-3 Plants
• Calvin Cycle = C3 Pathway• All plants use Calvin Cycle, but some plants ONLY
use C3 pathway– 95% of plants are this way
• Inefficient – lose some energy to heat– 30% on the best sunny day– In Photorespiration rubisco uses O2 instead of CO2 as
a substrate– Stomates open, O2 diffuses out, CO2 is used– Hot dry climates, stomates cannot stay open – lost
water, O2 builds up, photorespiration takes over
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C4 Plants
• C4– adaptation to help minimize photorespiration (1% of plants)
• C4 Plants – Separate light reactions and Calvin Cycle into different cells– Light reactions and carbon fixation– mesophyll
– CO2 combines with 3 carbon molecule to make 4 carbon – C4
– C4 – (malate) moves to bundle sheath cells, rest of Calvin Cycle
• Bundle sheath cells NOT exposed to 02
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C3 and C4 Plant Anatomy
C4 plantC3 plant
Vein
Stoma
Mesophyll
cell
Bundle-
sheath cell Mesophyll
cell
Stoma
Vein
Bundle-
sheath cell
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CAM Plants
• Occurs in desert plants (3-4% of plants)
• Only open stomates at night to fix CO2, then fix again during the day using Calvin Cycle
– Store night time CO2 as malate in vacuoles
– Stomates open, malate to chloroplast, release CO2, used in Calvin Cycle
• Happens in same cells
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C3 plant C4 plant CAM plant
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Global Climate Change
Green house effect: radiant heat trapped by CO2
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Global Climate Change
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Global Climate Change
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Global Climate Change
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Global Climate Change
• What would you do to curb climate change?