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    LECTURE ONPHOTOSYNTHESIS

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    By the end of this lecture you will be able to:

    1. Understand that ENERGY can be transformed from one form to another.2. Know that energy exist in two forms; free energy - available for doing work

    or as heat - a form unavailable for doing work.

    3. Appreciate that the Sun provides most of the energy needed for life onEarth.

    4. Explain why photosynthesis is so important to energy and material flow forlife on earth.

    5. Know why plants tend to be green in appearance.

    LEARNING OBJECTIVES

    . quate t e organe e o p otosynt es s n eu aryotes w t t e c orop ast.7. Describe the organization of the chloroplast.8. Understand that photosynthesis is a two fold process composed of the

    light-dependent reactions (i.e., light reactions) and the lightindependent reactions (i.e. Calvin Cycle or Dark Reactions).

    9. Tell where the light reactions and the CO2 fixation reactions occur in the

    chloroplast.10. Define chlorophylls giving their basic composition and structure.11. Draw the absorption spectrum of chlorophyll and compare it to the action

    spectrum of photosynthesis.12. Define the Reaction Centers and Antennae and describe how it operates.13. Describe cyclic photophosphorylation of photosynthesis.

    14. Describe noncyclic photophosphorylation of photosynthesis.

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    Energy can be transformed fromone form to another

    FREE ENERGY

    (available for work)vs.

    HEATnot available for work

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    THE SUN: MAIN SOURCE OFENERGY FOR LIFE ON EARTH

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    Almost all plants are photosynthetic autotrophs, asare some bacteria and protists

    Autotrophs generate their own organic matter through

    photosynthesis Sunlight energy is transformed to energy stored in the

    form of chemical bonds

    THE BASICS OF PHOTOSYNTHESIS

    (a) Mosses, ferns, andflowering plants

    (b) Kelp

    (c) Euglena (d) Cyanobacteria

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    Light Energy Harvested by Plants &Other Photosynthetic Autotrophs

    6 CO2 + 6 H2O + light energy C6H12O6 + 6O2

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    Food Chain

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    THE FOOD WEB

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    WHWHY ARY ARE PE PLANLANTS GTS GREREEN?EN?

    It's not that easy bein' greenHaving to spend each day the color of the leavesWhen I think it could be nicer being red or yellow or goldOr something much more colorful like that

    Kermit the Frog

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    Electromagnetic Spectrum andVisible Light

    Gammarays X-rays UV

    Infrared &Microwaves Radio waves

    Visible light

    Wavelength (nm)

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    Different wavelengths of visible light are seen by

    the human eye as different colors.

    WHYWHY AREARE PLAPLANTSNTS GREGREEN?EN?

    Gamma

    raysX-rays UV Infrared

    Micro-

    waves

    Radio

    waves

    Visible light

    Wavelength (nm)

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    The feathers of male cardinals

    are loaded with carotenoidpigments. These pigmentsabsorb some wavelengths of

    light and reflect others.

    Sunlight minus absorbedSunlight minus absorbedwavelengths or colorswavelengths or colorsequals the apparent colorequals the apparent colorof an object.of an object.

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    Why are plants green?

    Transmitted light

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    WHYWHY AREARE PLAPLANTSNTS GREGREEN?EN?

    Plant Cells

    have GreenChloroplasts

    The thylakoidmembrane of thechloroplast is

    impregnated withphotosyntheticpigments (i.e.,chlorophylls,carotenoids).

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    Chloroplastsabsorb lightenergy and Light

    Reflectedlight

    THE COLOR OF LIGHT SEEN IS THE

    COLOR NOT ABSORBED

    convert it tochemical energy

    Absorbedlight

    Transmittedlight

    Chloroplast

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    Photosynthesis is the process by which

    autotrophic organisms use light energy tomake sugar and oxygen gas from carbon

    AN OVERVIEW OF PHOTOSYNTHESIS

    Carbondioxide

    Water Glucose Oxygengas

    PHOTOSYNTHESIS

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    Th lvin l m k

    Light

    Chloroplast

    LightCalvinc cle

    NADP++++

    ADP+ P

    The light reactionsconvert solarenergy to chemical

    energy Produce ATP & NADPH

    AN OVERVIEW OF PHOTOSYNTHESIS

    sugar from carbondioxide ATP generated by the light

    reactions provides the energyfor sugar synthesis

    The NADPH produced by thelight reactions provides theelectrons for the reduction of

    carbon dioxide to glucose

    reactions

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    Chloroplasts: Sites of Photosynthesis Photosynthesis

    Occurs in chloroplasts, organelles in certainplants

    All green plant parts have chloroplasts and carry

    out p otosynt es s The leaves have the most chloroplasts

    The green color comes from chlorophyll in thechloroplasts

    The pigments absorb light energy

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    In most plants, photosynthesis occursprimarily in the leaves, in the chloroplasts

    A chloroplast contains:

    stroma, a fluid

    Photosynthesis occurs in chloroplasts

    grana, stacks of thylakoids The thylakoids contain chlorophyll

    Chlorophyll is the green pigment that captures

    light for photosynthesis

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    The location and structure of chloroplasts

    LEAF CROSS SECTION MESOPHYLL CELL

    LEAF

    Chloroplast

    Mesophyll

    CHLOROPLAST Intermembrane space

    Outermembrane

    Innermembrane

    Thylakoid

    compartmentThylakoidStroma

    Granum

    StromaGrana

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    Chloroplasts contain several pigments

    Chloroplast Pigments

    Chlorophyll a Chlorophyll b

    Carotenoids

    Figure 7.7

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    Chlorophyll a & b Chl a has a methylgroup

    Chl b has a carbonylgroup

    orp yr n r ngdelocalized e-

    Phytol tail

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    Different pigments absorb light

    differently

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    Excitedstate

    e

    Heat

    Light

    2

    Excitation of chlorophyll

    in a chloroplast

    Loss of energy due to heatcauses the photons of light to be

    less energetic.Less energy translates into

    longer wavelength.

    Energy = (Plancks constant) x

    (velocity of light)/(wavelength oflight)

    Transition toward the red end of

    the visible spectrum.

    e

    Photon

    Light

    (fluorescence)

    Chlorophyllmolecule

    Groundstate

    (a) Absorption of a photon

    (b) fluorescence of isolated chlorophyll in solution

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    Primaryelectron acceptor

    Molecular Game of Hot Potato

    Photon

    Reactioncenter

    PHOTOSYSTEM

    Pigmentmolecules

    of antenna

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    Cyclic Photophosphorylation Process for ATP generation associated with

    some Photosynthetic Bacteria

    Reaction Center => 700 nm

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    Two types of

    photosystemscooperate in the

    Water-splittingphotosystem

    NADPH-producingphotosystem

    ATPmill

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    Primaryelectron acceptor

    Primaryelectron acceptor

    Noncyclic Photophosphorylation Photosystem II regains electrons by splitting

    water, leaving O2 gas as a by-product

    Photons

    PHOTOSYSTEM I

    PHOTOSYSTEM II

    Energy forsynthesis of

    by chemiosmosis

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    The O2 liberated by photosynthesis is made

    from the oxygen in water (H+

    and e-

    )

    Plants produce OPlants produce O22

    gas by splitting Hgas by splitting H22OO

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    Primaryelectronacceptor

    Energyto make

    Primaryelectronacceptor

    Light

    NADP++++

    2

    3

    How the Light Reactions Generate ATP and NADPH

    2 H++++ + 1/2

    Water-splittingphotosystem

    Reaction-center

    chlorophyll

    Light

    Primaryelectronacceptor

    NADPH-producingphotosystem1

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    Two connected photosystems collectphotons of light and transfer the energy to

    In the light reactions, electron transportIn the light reactions, electron transportchains generate ATP, NADPH, & Ochains generate ATP, NADPH, & O22

    The excited electrons are passed from theprimary electron acceptor to electron

    transport chains Their energy ends up in ATP and NADPH

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    The electron transport chains are arranged

    with the photosystems in the thylakoidmembranes and pump H+ through that

    Chemiosmosis powers ATP

    synthesis in the light reactions

    The flow of H+ back through the membrane isharnessed by ATP synthase to make ATP

    In the stroma, the H+ ions combine with NADP+

    to form NADPH

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    The production of ATP by chemiosmosis in

    photosynthesis

    Thylakoidcompartment(high H+) Light Light

    Thylakoid

    membrane

    Stroma(low H+)

    Antennamolecules

    ELECTRON TRANSPORTCHAIN

    PHOTOSYSTEM II PHOTOSYSTEM I ATP SYNTHASE

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    A Photosynthesis Road Map

    Chloroplast

    Light

    Stack ofthylakoids ADP

    + P

    NADP++++

    Stroma

    reactions cycle

    Sugar used for

    Cellular respiration

    Cellulose

    Starch

    Other organic compounds

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    Review: Photosynthesis uses lightenergy to make food molecules

    Light

    Chloroplast A summary of

    the chemical

    Photosystem IIElectrontransport

    chainsPhotosystem I

    CALVINCYCLE Stroma

    LIGHT REACTIONS CALVIN CYCLE

    Cellularrespiration

    Cellulose

    Starch

    Otherorganiccompounds

    photosynthesis

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    It's not thatIt's not that

    easy bein'easy bein'

    is essential foris essential for

    life on earth!life on earth!