biology 161 - urinalysis [email protected]

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Biology 161 - Urinalysis [email protected]

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Page 1: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Biology 161 - Urinalysis

[email protected]

Page 2: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Kidney Structures

Ureter

Renal Artery

Renal Vein

Renal Capsule

Page 3: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Kidney Structures

Renal Calyx

Renal Pyramid

Renal Sinus

Glomerulus

Page 4: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Kidney Structures

Renal CortexRenal Medulla

Page 5: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Kidney Structures

Renal Calyx

Renal Cortex

Renal Pyramid

Renal Capsule

Page 6: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Kidney Structures

Renal Hilum

Renal Capsule(Covering)

Page 7: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

1.) Test for pH (Bayer Sticks)• Normal pH of urine is 4.5 – 8.0

2.) Color and Turbidity • Normal urine is pale yellow and clear

Page 8: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

3.) Specific Gravity of Urine – specific gravity compares the weight of the urine to the weight of water.

• Water is assigned a specific gravity of 1.00• Urine has a normal specific gravity of 1.003 to 1.035• High specific gravity indicates dehydration• Low specific gravity may indicate diabetes insipidus.

Page 9: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

Specific Gravity Cont. How to Read the urinometer1. Fill the cylinder ¾ full of

urine.2. Place the hydrometer into the

urine.3. Read the scale at the top of

the urine.Midget Urinometer

Page 10: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

4.) Reading the multistix.1. Completely insert the

multistix into the urine.2. Read multistix from top to

bottom on the chart.Note – Do not let the

multistix touch the chart itself while reading it

Page 11: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

Possible Multistix Positives• Blood – may occur with burns, crushing

injuries, hemolytic anemia.• Ketones – may occur with diabetic acidosis.• Glucose – may occur with diabetes mellitus or

with extra intake of sugar.• Protein – may occur with chronic renal failure.

Page 12: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

• The pH – should be in the 4.5 to 8.0 range• Bilirubin – increase with bile duct blockages• Urobilinogen – with total obstruction of bile

flow, no bilirubin reaches the intestines to be worked on by bacteria and changed to urobilinogen in the urine. Urine tends to be pale since urobilinogen colors the urine.

Page 13: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

Benedict’s – tests for the presence of a monosaccharide (reducing sugar) or in our bodies case glucose. The solution will turn from blue to brick red in the presence of glucose.

Page 14: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

Biuret – Tests for the presence of protein in solution. In the presence of protein the solution will turn from blue to violet in color.

Page 15: Biology 161 - Urinalysis Scott.lehbauer@lethbridgecollege.ab.ca

Urinalysis

Sulfur – will test for the presence of bile in the urine. If the pinch of sulfur floats in the urine then there is no bile present. If the sulfur sinks and becomes frothy then there is bile present in the urine.