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BIOFILMSRESEARCH CENTER FOR BIOINTERFACES
AnnuAl RepoRt 2013
MALMÖ UNIVERSITYBIOFILMS – RESEARCH CENTER FOR BIOINTERFACES
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Biofilms – Research Center for Bio inter faces
is a translational re search center covering
four research groups within three faculties
at Malmö University. The core strengths
of the Center are our broad expertise,
spanning the whole range from theoretical
modelling to clinical sciences, and our long
experience in working with industry and
relating to their needs. The general aim
of research activities at the Center is to
understand, predict and control material/
cell/tissue interactions with medical, dental,
food and environmental applications.
Biofilms – Research Center for Bio inter faces
aims to become a regional site for a creative
biomedical technology environment in the
Öresund region, facilitating collaboration
between academic research, higher educa-
tion and industry, based on pharmaceutical
technology, biotechnology and medical
technology. Together, they form a key area
that offers significant commercial growth
potential. We are striving to become a
prime entry point for regional industry
seeking translational academic expertise
in the biomedical technology field.
CENTER MISSION STATEMENT
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Annual Report 2013
AbbReviAtions
CONTENTS
Mah: Malmö University; LU: Lund University; LTH: Faculty of Engineering, Lund
University, KTH: The Royal Institute of Technology; LuTH: Luleå University of
Technology; UU: Uppsala University; LiU: Linköping University; KI: Karolinska
Institute; GU: Gothenburg University; OD: Faculty of Odontology; HS: Faculty of
Health and Society; TS: Faculty of Technology and Society. KKs: The Knowledge
Foundation; VR: The Swedish Research Council; EU: European Union; FP7: 7th
Framework Programme; SI: Swedish Institute; PI: Principal Investigator; BRCB:
Biofilms – Research Center for Biointerfaces
DIRECTOR’S REpORT 4
NEwS 2013 5
LIST OF CENTER MEMBERS 8
RESEARCH HIgHLIgHTS 13
LIST OF RESEARCH ACTIvITIES 26
SCIENTIFIC INSTRuMENTATION AND EquIpMENT
30
COLLABORATIvE pARTNERS OF THE CENTER
37
wORkSHOpS AND CONFERENCES ORgANISED By THE CENTER
42
SEMINARS AT THE CENTER 44
pHD THESES SupERvISED By CENTER MEMBERS
46
LIST OF puBLICATIONS AT THE CENTER FROM 2013
49
CONTRIBuTIONS TO AND pARTICIpATION IN CONFERENCES AND wORkSHOpS
58
front illustration: Image of
dehydrated human unpurified
saliva in air obtained using
polarized light microsopy (yana
Znamenskaya, phD Thesis “Effect
of Hydration on Thermodynamic,
Rheological and Structural
properties of Mucin” 2013)
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DIRECTOR’S REpORT
In October, I returned as Director of the Center after a year’s break, during which I was project manager for the Malmö University strategic platform. I would now like to take the opportunity to reflect on the past year on behalf of myself and my predecessor Johan Drott, who has moved on to new challenges. 2013 has been a busy year for the Center and operations have developed positively on many fronts.
On the staff side, 2013 has been an exciting year. Our newly recruited Professor Börje Sellergren has moved his entire operation to Malmö University and he has now settled in with his research group. Liselott Lindh became a full professor at the Department of Prosthetic Dentistry, Sergey Shleev became a full profes-sor at the Department of Biomedical Science and Krister Thuresson became asso ciate professor at the Department of Biomedical Science. Alma Mašić, Sebastian Björklund and Yana Znamenskaya all successfully defended their PhD theses. We are especially proud that Alma Mašić’s thesis “Investigation of a Biofilm Reactor Model with Suspended Biomass” was awarded a prize at Malmö University’s Annual Ceremony. The Center has also been privileged to have Cristina Glad as Chair of the Steering Committee. Cristina Glad was appointed Chair by the Pro Vice-Chancellor in January 2013 and, together with the other members of the Steering Committee, she has made a very positive and constructive contri-bution to the Center’s strategic development.
As regards the strategic development of the Center, I would like to mention that during 2013 Malmö University signed an agreement for the new laboratory facilities adjacent to Medeon in Malmö. This will give the Center an excellent basis for realising the vision
of becoming a regional site for a creative environment for biomedical technology, based on pharma ceutical technology, biotech nology and medical technology, and facilitating collaboration between academic research, higher education and industry.
During 2013, we had a portfolio of 35 research projects and collaborated with 30 dif-ferent companies. We successfully established new research collaborations with both local and international industrial companies. We produced around 90 scientific publications in peer-reviewed scientific journals and research-ers at the Center actively contributed to the national and international research com-munities through involvement in organising international meetings, including the 8th International Conference on PBL in Dentistry, The International HILIC Day and the 9th Annual Workshop of Biofilms – Research Center for Biointerfaces. Other contributions to the research community have been made through membership of editorial and scien-tific advisory boards and as guest editors for highly reputed international journals, such as ‘International Journal of Prosthodontics and Restorative Dentistry’, ‘American Journal of Dentistry, ‘ScienceJet’, ‘Non-Linear Analysis: Modelling and Control’, and ‘Colloids and Surfaces: A’.
Finally, I would like to thank all those with whom we have worked for their invaluable contribution during the past year and Malmö University for their consistent support, as well as the Knowledge Foundation, the Swedish Research Council, the Borrow Foundation, the Swedish Laryng Foundation, the European Commission, and the Swedish Institute who funded our research.
Anna Holmberg, Director Biofilms
– Research Center for Biointerfaces
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NEwS 2013
Liselott Lindh derives her energy from her contact with dental students and patient treatments in the clinic. She graduated as a dentist in 1981, became certified specialist in Prosthetic Dentistry 1992 and defended her thesis in 2002. Liselott is now appointed as Professor of Prosthetic Dentistry at the Faculty of Odontology.
“There was never any other alternative for me than to become a dentist. It was a profession that combined my interest in crafts and to help people both in terms of oral health and quality of life,” she says.
Liselott Lindh’s research interests started from the complaints the patients were telling her, and her curiosity was the driving force to try to find an explanation to what could be the reason. Her research profile is oral surface phenomena with the aim to improve oral health and includes investigations regarding:
• saliva, blood and saliva molecular surface activity on different dental materials, and salivary pellicle composition and barrier functions in healthy, erosion and dry mouth patients,
• surface modification of dental materials for improving the oral health among the spare part human and vulnerable patient groups,
• development of saliva substitutes, and • clinical studies related to the aforemen-
tioned areas of interest.
“I meet many patients who have lost their teeth, for instance after radiotherapy, and who are unable to produce their own saliva. For instance, patients wearing removable dentures in combination with the lack of saliva, frequently experience soreness in the dry and fragile mucosa due to the wear from the dentures. There is still a lot to be done for these patients to increase their oral func-tion and comfort and thus their life quality.”
newly Appointed pRofessoR with A focus on oRAl suRfAce phenomenA And oRAl heAlth
1.
newly Appointed pRofessoR with A pAssion foR bioelectRonics
2.
With a burning interest in science, Sergey Shleev has never been in any doubt that research was to be his future. He became pro-fessor in 2013. Shleev has conducted research concerning biological electric power sources over a long period and his general field of interest is bioelectronics.
“We have recently accomplished a major EU project in which we produced and tested a self-contained (self-powered and wireless) device for diabetics. Using a biofuel cell, a sensor, and a transmitter, the blood sugar level can be monitored and a signal can be sent to your mobile telephone when it is time
for an insulin injection. We’re well on the way and we’re now applying for funding to proceed and improve the device, specifically to miniaturize it.”
At the same time, he and his colleagues have a discovery in the pipeline.
“We have produced a new and unique source of electrical energy; a discovery that we are seeking to patent. The energy source is eco-friendly and there is an extremely broad field of use, from road vehicles to implants. We are also considering commercialising it at some point in the future.”
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3. KRisteR thuResson new AssociAte pRofessoR
Krister Thuresson conducts research into self-association and aggregation of surface-active substances. He is happiest working at the border between academia and industry.
“It is a matter of understanding what hap-pens in solutions and at interfaces,” he says. “A typical example is viscosity modifiers and stabilisers, which have an important role to play in applications ranging from paint formu-lations to eye operations. With fundamental knowledge you have the means to change and create smart solutions with robust properties.”
Krister Thuresson combines research with working at a company that manufactures creative materials, such as novel modelling
compounds for children. “As an associate professor I will consolidate my platform in academia. I will continue to supervise PhD students and degree projects and take part in teaching. By doing so, I would like to impart the feeling of what it is like as an academic to move into industry, and back. It all comes down to capitalising on your unique characteristics.”
Having ingenious ideas is for the most part not enough. It is also necessary for funding to be in place, that the idea fits into the company’s profile and, not least, that the prospective product will satisfy the market and all the legal requirements.
4. wAstewAteR systems in focus foR AwARd-winning thesis
Alma Mašić’s thesis “Investigation of a Biofilm Reactor Model with Suspended Biomass” was awarded the Malmö University prize for the best thesis. Her research has been heralded as a milestone in the development of math-ematical models and the work could lead to improved wastewater treatment and thus a better environment in the future.
“It perhaps sounds strange but it is possible with the aid of mathematics to study a course of events without needing to take samples and conduct experiments every time – simulations can be made instead. This makes it consider-ably easier to acquire an overview and making
analyses becomes much more accessible,” says Alma Mašić.
Alma Mašić’s thesis deals with how suspended biomass interacts with biofilms at wastewater treatment plants. She is now a postdoc at Eawag, a Water Research Institute in Zürich. Alma Mašić came to Biofilms – Research Center for Biointerfaces after studying at Stockholm University. “The biofilm project attracted me and it sounded extremely exciting. There are very few biofilm research centres in Europe.”
5. new chAiR of bRcb
Cristina Glad, former acting President of Bioinvent International, was appointed during the year to chair the steering committee for Biofilms – Research Center for Biointerfaces (BRCB).
“It will be extremely exciting. I hope to be involved in bringing research into the commu-nity,” she said prior to taking up the position.
Cristina Glad is a biochemist and took a PhD at Lund University with a thesis on an immunochemical analysis method. After a number of years in academia, she continued her career at Bioinvent. The company, at which Glad held several leading positions and during 2013 was acting President, is a listed company and develops antibody-based pharmaceuticals.
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Researchers from BRCB began moving into the new premises in the Medeon area of Malmö at the end of the autumn.
“The premises are large and have very well-equipped laboratories. BRCB has expanded significantly in recent years and the new premises have come at the right time in the light of the changes that lie ahead,” says Anna Holmberg, Director.
The new premises will be an important research and innovation environment.
“Our aim is to create a meeting point for researchers, entrepreneurs and business own-ers,” says Anna Holmberg.
The new premises have advanced instru-ments and good analytical equipment. The researchers now have access to instruments such as LCMS, LCMSMS, GCMS and NMR. “We have not had access to this type of analytical equipment at Malmö University previously.”
“The move is important if research is to
continue to develop. With the new premises, BRCB’s potential to achieve its goal of becom-ing a regional meeting point for a creative environment for biomedical technology in the Öresund region, facilitating collabora-tion between academia, higher education and industry, based on pharmaceutical technology, biotechnology and medical technology. We are striving to become a prime entry point for regional industry seeking translational academic expertise in the biomedical technol-ogy field,” states Anna Holmberg.
The first to move in will be Professor Börje Sellergren’s research group, who are working with molecular imprinting. “There is already considerable interest among regional companies in using our equipment. Ultimately, it could be possible to share prem-ises, creating a situation where academics and industry representatives would be working side by side.”
Anna Holmberg is convinced that the new premises will benefit both BRCB’s own research projects as well as collaboration with industry.
“I have had contact with BRCB in recent years and I know a number of the researchers from previously. Many centres of this nature fail to survive when the first few years of funding
come to an end. BRCB, however, has suc-ceeded in finding a structure and has contin-ued to develop. It is extremely exciting to be involved and make a contribution,” she says.
bRcb moves to new pRemises AdvAnced lAboRAtoRies in the medeon AReA will open up new oppoRtunities.
6.
During the autumn, Malmö University gained access to new premises in the Medeon area. The first to move in are researchers at Biofilms – Research Center for Biointerfaces (BRCB). Anna Holmberg, Director of the Center, can foresee major opportunities now that the researchers have access to well-equipped laboratories.
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LIST OF CENTER MEMBERS
1. mAnAgement And boARdssteeRing committee
Cristina Glad
Chair
CEO C glad Consulting AB
Thomas Arnebrant
Representative Faculty of Health and Society
Gunnel Svensäter
Representative Faculty of Odontology
Karl Obrant
Head of Research, Skåne university Hospital
Markus Johnsson
Senior Director, pharmaceutical Development, Camurus AB
Ulf Brogren
CEO promimic AB
Peter Nordström
Senior project Manager,
Medicon valley Alliance
senioR ReseARcheRs foRum
Johan Drott /Anna HolmbergChairphD, Director
Ann Wennerbergprofessor
Liselott Lindhprofessor
Gunnel Svensäterprofessor
Julia Daviesprofessor
Bertil KinnbyAssoc. professor
Claes WickströmAssoc. professor
Per Jönssonprofessor
Christina Bjerkén
Assoc. professor
Tautgirdas Ruzgasprofessor
Thomas Arnebrantprofessor
Vitaly Kocherbitov
Assoc. professor
Johan Engblom
Assoc. professor
Anette Gjörloff-Wingren
Assoc. professor
Maria Stollenwerk
phD
During 2013, the Center comprised the following members.
2.
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Biofilms – Research Center for Biointerfaces
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peRmAnent stAff
Anna Holmberg
phD, Director from October, 2013
Johan Drott, PhD
Director January–May, 2013
Thomas Arnebrant
professor, Acting Director May–October, 2013
Alexei Iantchenko
professor
Ann Wennerberg
professor
Börje Sellergren
professor
Gunnel Svensäter
professor
Håkan Eriksson
professor
Julia Davies
professor
Lennart Ljunggren
professor
Liselott Lindh
professor
Per Jönsson
professor
Sergey Shleev
professor
Tautgirdas Ruzgas
professor
Anette Gjörloff-Wingren
Assoc. professor
Bertil Kinnby
Assoc. professor
Christina Bjerkén
Assoc. professor
Claes Wickström
Assoc. professor
Johan Engblom
Assoc. professor
Ryo Jimbo
Assoc. professor
Vitaly Kocherbitov
Assoc. professor
Zoltan Blum
Assoc. professor
Gabriela Enggren
phD
Jakob Blomqvist
phD
Javier Sotres
phD
Jessica Neilands
phD
Lars Ohlsson
phD
Maria Stollenwerk
phD
Tove Sandberg
phD
Yuanji Cheng
phD
3.
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JunioR ReseARcheRs And post-docs
Alejandro Barrantes
phD
Anna Runnsjö
phD
Gesche Graf
phD
Hala Ghandour Gari
phD
Jildiz Hamit Eminovski
phD
Jonas Carlstedt
phD
Justas Barauskas
phD
Lars Söderberg
phD
technicAl And AdministRAtive stAff
Eva Nilsson
Administrative coordinator
Agnethe Henriksson
Technician
Madeleine Blomqvist
Technician
Ulrika Troedsson
phD, Technician
Peter Falkman
phD, Research engineer
4.
Maria Falck Miniotis
phD
Pär Olsson
phD
Soma Ghosh
phD
Sudhirkumar Shinde
phD
5.
phd students
Alma Mašić (enrolled at Lu)
Anton Fagerström
Cathrine Albér
Celina Wierzbicka
Deyar Mahmood
Dmitrii Pankratov
Francesca Cecchinato
Jonas Anderud
Liselott Ellmarker-Löfquist
Magnus Falk
Marija Jankunec
(enrolled at vilnius university, vilnius, Lithuania)
Mariko Hayashi
Marjan Dorkhan
Mark Galat
Michael Braian
Patrick Seumo
(enrolled at university of yaounde I, yaounde, Cameroon)
Peter Lamberg
6.
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Biofilms – Research Center for Biointerfaces
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guests
Thomas Zemb
Institute for Separation Chemistry
of Marcoule, France
Sergei Gavryushov
Russian Academy of Sciences, Moscow,
Russia
Robert Burger
DTu Nanotech, Copenhagen
Marco Donolato
DTu Nanotech, Copenhagen
Aušra Linkevičiūtė
vilnius university, Lithuania
Galina Pankratova
Bryansk State university, Bryansk, Russia
Wolfgang Bäther
Dräger Ag, germany
Stefan Lehman
Dräger Ag, germany
Anthony Rees
Biotage AB, Sweden
Jenifer Thewalt
SFu, vancouver, Canada
Ben Boyd
Monash university, Australia
Per Claesson
The Royal Institute of Technology, Stockholm,
Sweden
Malin Sjöö
Faculty of Engineering, Lund university,
Sweden
Per Hansson
uppsala university, uppsala, Sweden
Gordon Proctor
kings College, London, uk
Nayab Chaudhury
kings College, London, uk
Mogens Kilian
Aarhus university, Denmark
Tracey Winning
university of Adelaide, Australia
7.
Pär Johansson
Ramesh Chowdhary
Ricardo Trindade
Sebastian Björklund
(enrolled at Lu)
Silvia Galli
Sing Yee Yeung
Tuerdi Maimaitiyili
(enrolled at Lu)
Ulf Hejman
(enrolled at Lu)
Vida Krikstolaityte
(enrolled at vilnius university, vilnius,
Lithuania)
Yana Znamenskaya
Zahra El-Schich
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Biofilms – Research Center for Biointerfaces
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Charles Schuler
university of British Columbia, Canada
Henk Schmidt
Erasmus university, the Netherlands
Magnus Fornbacke
Enzymatica AB, Sweden
Mats Clarsund
Enzymatica AB, Sweden
Nigel Borrow
The Borrow Foundation, uk
Andrew Rugg-Gunn
Newcastle university, uk
Svante Twetman
Copenhagen university, Denmark
Per Rabe
Hallands Läns Landsting, Sweden
Barbera Musi
gambro AB, Lund, Sweden
Torbjörn Linden
gambro AB, Lund, Sweden
Helena Jeppson
gambro SB, Lund, Sweden
Anders Wieslander
gambro SB, Lund, Sweden
Jan Hall
Nobel Biocare, gothenburg, Sweden
Carina Norberg
public Oral Health Care Service, Blekinge,
Sweden
Christina Stebring
public Oral Health Care Service, kronoberg,
Sweden
Maria Hildning
public Oral Health Care Service, kronoberg,
Sweden
Margareta Elfvin
public Oral Health Care Service, kalmar,
Sweden
Amina Basic
university of gothenburg, Sweden
Andreas Sonesson
Lund university, Sweden
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
Biofuel cells (BFCs) produce electrical
energy by oxidizing glucose, lactate,
ethanol, etc., i.e., energy rich bioorganic
molecules present in biological tissues. To
facilitate the oxidation BFCs exploit bio-
logical catalysts, e.g., enzymes and living
cells immobilized at the electrodes. BFCs
based on three-dimensional (3D) nano-
structures loaded with enzymes represent
one of the most compact BFC designs. 3D
nanostructures are usually obtained by
modifying BFC electrodes with nanomate-
rials, such as carbon nanotubes or metallic
nanoparticles. The main advantages of
BFCs are the possibility to fuel the devices
with renewable substrates and a relatively
easy realization of implantable design.
Performance of implanted BFCs in animals
has been studied and reviewed (Falk et
al. 2013). However, demonstrating the
performance of BFCs in implanted situa-
tions rarely address their biocompatibility
issue down to cellular level.
Recently, we have conducted the very
first study of a BFC in cell culture. BFC
comprised Corynascus thermophilus
cellobiose dehydrogenase (CDH) based
bioanode and Myrothecium verrucaria
bilirubin oxidase (BOx) based biocathode.
The electrodes were constructed at the
bottom of a cell culture plate (Figure 1).
The BFC had a power density of 25 µW
cm-2 at 0.5 V potential in simple buffer
solution and in cell culturing medium.
L929 murine fibroblast cells were seeded
on top of the BFC and possible effects of
the BFC on the cells and vice versa were
studied. It was shown that on average the
power of the BFC drops by about 70%
under a nearly confluent layer of cells. The
BFC appeared to have a toxic effect on the
L929 cell line (Figure 2). It was concluded
that the bioanode, consisting of CDH,
produced hydrogen peroxide at toxic
concentrations. However, the toxic effect
was circumvented by co-immobilizing
catalase on the bioanode.
RESEARCH HIgHLIgHTS
peRfoRmAnce of enzymAtic fuel cell in cell cultuRe
Falk, M., Villarrubia, C., Babanova, S., Atanassov, P., Shleev, S. (2013) Biofuel Cells for Biomedical Applications: Colonizing the Animal Kingdom. ChemPhysChem 14(10), 2045–2058.Lamberg, P., Shleev, S., Ludwig, R., Arnebrant, T., Ruzgas, T. (2014) Performance of enzymatic fuel cell in cell culture. Biosens. Bioelectron. 55, 168–173.
contact: [email protected]
figure 2. Image of L929 cells close to the edge of the anode electrode. It can be seen that L929 cells are more round at the edge of the electrode (black) indicating the toxicity of BFC. In this study it was determined that peroxide is produced as a side reaction of CDH at the anode. The toxic effect was circumvented by co-immobilizing catalase together with CDH.
figure 1. Construction of biofuel cell (BFC) at the bottom of cell culture plate.
1.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
Miniature, self-contained biodevices
powered by biofuel cells may enable a
new generation of implantable, wireless,
minimally invasive neural interfaces for
neurophysiological in vivo studies and
for clinical applications. We report on the
fabrication of a direct electron transfer
based glucose/oxygen enzymatic fuel cell
from genuinely three-dimensional nano-
structured microscale gold electrodes,
modified with suitable biocatalysts. We
show that the process underlying the
simple fabrication method of three-
dimensional nanostructured electrodes
is based on an electrochemically driven
transformation of physically deposited
gold nanoparticles. We experimentally
demonstrate that mediator-, cofactor-,
and membrane-less enzymatic fuel cells
do operate in cerebrospinal fluid and in
the brain of a rat, producing amounts of
electrical energy sufficient to drive a self-
contained biodevice, viz. 7 µW cm-2 in
vitro and 2 µW cm-2 in vivo at an operating
voltage of 0.4 V. Last but not least, we
also demonstrate an inductive coupling
between three-dimensional nanobioelec-
trodes and living neurons.
enzymAtic fuel cells bAsed on micRoscAle nAnostRuctuRed electRodes opeRAting in vivo
2.
Andoralov V., Falk M., Suyatin D.B., Granmo M., Sotres J., Ludwig R., Popov
V.O., Schouenborg J., Blum Z., and Shleev S. (2013) Biofuel cell based on
microscale nano structured electrodes with inductive coupling to rat brain neurons.
Scientific Reports 3, 3270.
contact : [email protected]
Enzymatic fuel cell implanted into the rat brain (according to Andoralov et al., 2013). Close up pictures of the implantation with (left) and without (right) localised surface bleeding.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
Two blue multicopper oxidases (MCOs),
viz. Trametes hirsuta laccase (Lc) and
Myrothecium verrucaria bilirubin oxidase
(BOx), were immobilised on bare polycrys-
talline gold (Au) surfaces by direct adsorp-
tion from both dilute and concentrated
enzyme solutions. The adsorption was
studied in situ by means of null ellipso-
metry. Moreover, both enzyme modified
and bare Au electrodes were investigated
in detail by atomic force microscopy
(AFM), as well as electrochemically. When
adsorbed from dilute solutions (0.125 and
0.25 mg mL-1 in the case of Lc and BOx,
respectively) the amount of enzyme per
unit area was determined to be ca. 1.7
and 4.8 pmol cm-2, whereas the protein
film thickness was determined to be 29
and 30 Å for Lc and BOx, respectively. A
well-pronounced bioelectrocatalytic reduc-
tion of molecular oxygen was observed on
BOx/Au biocathodes, whereas this was not
the case for Lc modified Au electrodes, i.e.
adsorbed Lc was catalytically inactive.
The initially observed apparent catalytic
constants for the adsorbed BOx and the
enzyme in solution were found to be very
close to each other, viz. 54 s-1 and 58 s-1,
respectively (pH 7.4, 25ºC). However, after
3 h of operation of BOx/Au biocathodes
the apparent catalytic constant dropped
to 23 s-1. Based on experimental results,
conformational changes of the enzymes
(in all likelihood, their flattening on the Au
surface) were suggested to explain deactiva-
tion of MCOs on the bare Au electrodes.
Pankratov D., Sotres J., Barrantes A.,Arnebrant T., Shleev S. (2014) Interfacialbehaviour and activity of laccase andbilirubin oxidase on bare gold surfaces.Langmuir 30, 2943-2951.
contact: [email protected]
inteRfAciAl behAviouR And Activity of blue multicoppeR oxidAses on bARe gold suRfAces
3.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
Biofilm systems always have a certain
amount of suspended biomass present in
the reactor, mostly due to detachment of
biomass from the biofilm. The amount of
suspended biomass is generally smaller
than in a proper activated sludge reac-
tor, but may still influence the growth of
the biofilm as it is retained in the reactor
long enough for reattachment to occur.
Traditional mathematical biofilm models
typically do not include suspended bio-
mass even when the detachment process
is included, thereby assuming that any
effects due to suspended biomass can be
neglected. While this assumption can be
reasonable for certain lab-scale reactors, it
may be of critical importance for biofilm
reactors.
A mathematical and numerical analysis
of a dynamic one-dimensional model of
biofilm and suspended biomass in a con-
tinuous stirred tank reactor for a single
species single substrate setting, including
attachment and detachment, showed that
bacteria will either be completely washed
out or coexist as biofilms and suspended
biomass. Furthermore, suspended biomass
was found to be relatively more efficient
at substrate removal than biofilms, even
though the main contribution to removal
came from the biofilm, see Figure 1.
Masic, A. (2013) Investigation of a biofilm reactor model with suspended biomass.
PhD thesis, Lund University, Sweden.
Masic, A., Eberl, H.J. (2014) A modeling and simulation study of the role of
suspended microbial populations in nitrification in a biofilm reactor,
Bull. Math. Biol. 76(1):27-58.
Masic, A., Eberl, H.J. (2014) On optimization of substrate removal in a
bioreactor with wall attached and suspended bacteria, Math. Biosci. Eng. (to appear).
contact : [email protected]
investigAtion of A biofilm ReActoR model with suspended biomAss
4.
With an extended model for a consider-
ably more complex multi-species multi-
substrate nitrifying mobbing bed biofilm
reactor in a wastewater setting, it was
revealed through a computational study
that the incorporation of suspended bio-
mass is significant for detailed descriptions
of the internal processes and intermediate
steps of nitrification. However, suspended
biomass did not affect the overall reactor
performance and need not be considered
if such results are the sole objective. In
the above studies it was assumed that the
required amount of untreated wastewater
is always available, which is certainly not
always a correct assumption. Moreover,
some situations require only a certain
amount of wastewater to be treated as
quickly as possible. Therefore, an optimal
control problem was defined, where the
control was selected as the flow between a
storage tank, holding the untreated waste-
water, and a biological treatment reactor.
figure 1. percentage of substrate removal performed by suspended biomass (left) and amount of suspended biomass relative to total biomass (right), at steady state as a function of biofilm area for different dilution rates D.
17
Biofilms – Research Center for Biointerfaces
Annual Report 2013
The problem was constructed with two
objectives: to remove as much substrate as
possible and to treat the water as quickly
as possible. It was found that the potential
for reactor improvement by varying the
flow rate is relatively modest and mostly
due to an initial transient phase during
which the bacteria adjust to their sur-
roundings. Since such initial conditions
in a reactor are usually unknown, the
implementation of such a control would
also be impractical.
Reduced AdheRence of oRAl stReptococci but not soft-tissue cells to AnodicAlly-oxidized titAnium Abutment suRfAces
5.
Abutments of endosseous dental implants
protruding through the mucosa, are
exposed to microorganisms in saliva.
Early colonizers initiate biofilm formation
and promote binding of later colonizers,
leading to the development of complex
plaque. Inflammatory responses to plaque
biofilms can lead to breakdown of sup-
porting bone tissue and implant failure.
A cuff of keratinized mucosa adhering to
an abutment surface provides a barrier
to the passage of microorganisms into
the peri-implant connective tissues and
is thus regarded as a key factor for the
long-term success of implant therapy. We
have therefore investigated both biofilm
formation and soft-tissue cell adherence to
anodically-oxidized (AOX1 and AOX2)
titanium surfaces designed for use in
implant abutments.
Scanning electron microscopy showed a
nanoporous structure on the anodically-
oxidized surfaces and near-edge X-ray
absorption revealed a higher anatase
content than commerically pure titanium
(CpTi) (Fig 1). Human oral keratinocytes
and gingival fibroblasts both adhered well,
with no significant differences between the
anodically-oxidized and CpTi surfaces (Fig
2). The adhesion strength of keratinocytes
was greater than that of fibroblasts but
no differences between the anodically-
oxidized and the CpTi surfaces were seen
for either cell type.figure 1. NEXAFS spectra of CpTi control (black line) and anodically-oxidized (green and red lines) and the two crystal phases of titanium oxide; anatase and rutile.
figure 2. Adherence of human gingival fibroblasts and oral keratinocytes to CpTi, AOX1 and AOX2 surfaces. Adhered cells were stained with Live/Dead BacLight. Scale bars represent 50µm.
18
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Dorkhan M, Yucel-LindbergT, Hall J, Svensäter G, Davies JR. Adherence of
human oral keratinocytes and gingival fibroblasts to nanoporous titanium
surfaces. BMC Oral health (submitted).
Dorkhan M, Hall J, Uvdal P, Sandell A, Svensäter G, Davies JR. Adherence of oral
streptococci to crystalline anatase-rich titanium. Biofouling (submitted).
contact : [email protected]
Four early colonizing oral streptococci
(Streptococcus gordonii, Streptococcus
mitis, Streptococcus oralis and Streptococcus
sanguinis) showed significantly reduced
binding to the anodically-oxidized sur-
faces than to the CpTi control (Fig 3). In
ad dition, exposure to the bacteria, reduced
adherence of keratinocytes to all surfaces
as well as causing damage to the cells.
Thus anatase-rich, nanoporous titanium
surfaces prepared by anodic oxidation
appear to hold promise for use in dental
implant abutments since they can reduce
binding of oral streptococci while at
the same time allowing fibroblasts and
keratinocytes to attach to the surface.
figure 3. graphs showing mean coverage values ± SEM from three independent experiments expressed as % of coverage on the control surfaces, analyzed using students t- test (*p<0.05, **p<0.01 and ***p<0.001).
19
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Periodontitis is initiated by increased flow
of protein-rich gingival crevicular fluid
resulting from the inflammatory response
of the host to biofilm accumulation. This
ecological change favours bacteria with a
proteolytic phenotype. The bacterial pro-
teases and breakdown products contribute
to, and trigger, the inflammatory reaction
resulting in tissue breakdown.
The serpin plasminogen activator inhibitor
type 2 (PAI-2/Serpin B2) is an inhibitor
of serine proteases and has been detected
in very high concentrations in gingival
fluid. The aim of the present study was
thus to investigate possible interactions
between PAI-2 and proteases produced by
a multispecies bacterial consortium with
a composition reflecting conditions in a
periodontal pocket.
A multispecies bacterial consortium com-
prising ten strains was grown for seven
days. PAI-2 was added to the biofilm con-
sortium and incubated for 1 hour followed
by the addition of FITC-labeled casein or
a serine protease substrate (H-Gly-Pro-
AMC). After the incubation period the
fluorescence in the supernatant was meas-
ured and the biofilm cells were examined
in a confocal scanning laser microscope.
PAI-2 showed a concentration-dependent
inhibition of bacterial proteolytic activity.
To our knowledge this is the first time that
PAI-2 has been shown to inhibit bacterial
proteases. Given the high concentration
of PAI-2 in the gingival region, this might
indicate that PAI-2 plays a role for the
integrity of the epithelial barrier.
seRine pRoteAse inhibitoR pAi-2 inhibits bActeRiAl pRoteAses
6.
Neilands J, Svensäter G, Kinnby B. Serine protease inhibitor PAI-2 inhibits bacterial proteases. Manuscript
contact : [email protected]
figure 2. proteolytic activity in ten species biofilm consortium in the presence or absence of low/high molecular (LMw/HMw) weight pAI-2 showing approximately 50% inhibition compare to control.
figure 1 . Ten species biofilm consortium incubated with serine protease substrate (H-gly-pro-AMC) after incubation with/without pAI-2. Bacteria with proteolytic activity appear blue.
20
Biofilms – Research Center for Biointerfaces
Annual Report 2013
We have shown that L. fermentum adheres
well to surfaces coated with MUC5B
mucin. In biofilms of L. fermentum formed
in a MUC5B environment, the proportion
of protease active cells (47 ± 0.6% of
the population) was significantly greater
(p<0.01) than that in biofilms formed
in nutrient broth (0.4 ± 0.04% of the
population) (Fig 1). Comparison of the
surface proteomes of biofilm cells of L.
fermentum in a MUC5B environment with
those in nutrient broth using two-dimen-
sional electrophoresis (2DE) and mass
spectroscopy, showed that the enhanced
proteolytic activity was associated with
increased expression of a glycoprotease;
O-sialoglycoprotein endopeptidase, but
surprisingly the most significant changes in
expression were seen in chaperone proteins
such as DnaK and trigger factor.
We conclude that adhesion to mucin-
coated surfaces leads to a shift towards a
suRfAce-AssociAted chApeRones (tRiggeR fActoR And dnAK) ARe pivotAl foR incReAsed pRoteAse Activity in lActobAcillus feRmentum biofilms on humAn sAlivARy muc5b mucin
7.
Wickström C, Chávez de Paz L, Davies JR, Svensäter G. Surface-associated
MUC5B mucins promote protease activity in Lactobacillus fermentum biofilms
(2013) BMC Oral Health 13(1):43. DOI: 10.1186/1472-6831-13-43.
contact : [email protected]
Mucosal surfaces are coated with layers
of mucus gel that protect the underly-
ing tissues and promote colonization by
members of the commensal microflora.
Lactobacillus fermentum is a common
inhabitant of the oral cavity, gastrointes-
tinal and reproductive tracts and is one
of the most important lactic acid bacteria
contributing to the formation of a healthy
intestinal microflora.
more protease-active phenotype within L.
fermentum biofilms and that the enhanced
proteolytic activity was associated with an
increase in O-sialoglycoprotein endopepti-
dase on the cell surface. Upregulation of
chaperone proteins in the mucin environ-
ment may contribute to the proteolytic
phenotype through activation of the
glycopeptidase. This would represent one
way for commensal lactobacilli e.g. L. fer-
mentum to exploit complex substrates in
their local environment in order to survive
on mucosal surfaces.
figure 1. proteolytic activities of Lactobacillus fermentum biofilm populations. CSLM images showing biofilm cells growing in (a) a MuC5B environment or (b) a nutrient broth environment. proteolytic activity was visualized by incubation with a FITC-conjugated casein substrate (green) for 1 hour. Cells were counterstained with Syto 24 (red).
figure 2. 2DE of surface proteins from L. fermentum. proteins isolated from bacteria in a MuC5B-environment (left) or a nutrient broth environment (right) were subjected to IEF in a pH 4–7 gradient followed by SDS-pAgE in 14% gels. gels were silver-stained and image analysis of differently expressed proteins performed using Delta 2D software.
21
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Caries and periodontitis are biofilm-
mediated oral diseases, resulting from
complex interactions between the com-
mensal microbiota, host susceptibility and
environmental factors. Bacteria growing
in biofilms are often rather resistant to
antimicrobial substances and studies on
biofilm formation and architecture in
vivo are therefore important in order to
investigate the effect of antibacterial treat-
ments. However, harvesting undisturbed
plaque samples from teeth for viewing is
a practical challenge.
Here, we describe a novel method to study
dental plaque formation and the effects of
antibacterial measures in vivo. Discs of
human enamel were fixed to abutments
at two premolar implant sites in three
healthy volunteers. A biofilm was allowed
to develop for 7 days, after which one
control disc was removed. The patients
then rinsed with either sodium fluoride
or chlorhexidine and the treated disc was
also removed. The samples were analyzed
using an inverted confocal laser scanning
microscope (Nikon TE2000). Post pro-
cessing and image analysis was performed
with ImageTrak software (http://www.
ucalgary.ca/styslab/imagetrak).
The three subjects accumulated different
amounts of visible plaque over the test
period (Figure 1).
A novel method to hARvest undistuRbed oRAl biofilms foR confocAl lAseR scAnning micRoscope
8.
Rabe P, Twetman S, Kinnby B, Davies J, Svensäter G. A novel method to harvest undisturbed oral biofilms for confocal laser scanning microscopy. Manuscript.
contact : [email protected]
Bacterial vitality was high throughout
the control biofilms, with the highest
proportion of viable cells closest to the
enamel surface. Chlorhexidine exhibited
the greatest effect upon bacteria in the
upper layers of the biofilm, while fluoride
appeared to penetrate and exert an effect
in the deeper levels of the biofilm (Figure
2) No obvious effects upon biofilm thick-
ness were seen suggesting that the agents
did not remove the biofilms.
figure 2. Sagittal profiles showing the appearance of biofilms after rinsing with chlorhexidine and sodium fluoride.
figure 1. Different appearance of the control biofilms from three different subjects.
22
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Implant surface properties have long
been identified as an important factor to
promote osseointegration. The importance
of nanostructures and hydrophilicity on
osseointegration has gained much interest
in the last years. The aim of this study
was to investigate how nanostructures
and wettability influence osseointegration
and to identify whether the wettability, the
nanostructure or both in combination play
the key role in improved osseointegration.
study layout: Twenty-six adult rab-
bits each received two Ti grade 4 discs in
each tibia. Four different types of surface
modifications with different wettability
and nanostructures were prepared: hydro-
phobic without nanostructures (SLA), with
nanostructures (SLAnano); hydrophilic
with two different nanostructure densi-
ties (low density: pmodSLA, high density:
SLActive). All four groups were intended
to have similar chemistry and microrough-
ness. The surfaces were evaluated with
contact angle measurements, X-ray pho-
toelectron spectroscopy, scanning electron
microscopy, atomic force microscopy and
interferometry. After 4 and 8 weeks healing
time, pull-out tests were performed.
results: SLA and SLAnano were hydro-
phobic, whereas SLActive and pmodSLA
were super-hydrophilic. No nanostruc-
tures were present on the SLA surface,
but the three other surface modifications
clearly showed the presence of nanostruc-
tures, although more sparsely distributed
on pmodSLA. The hydrophobic samples
showed higher carbon contamination
levels compared with the hydrophilic
samples. After 4 weeks healing time,
SLActive implants showed the highest
pull-out values, with significantly higher
pull-out force than SLA and SLAnano.
After 8 weeks, the SLActive implants had
the highest pull-out force, significantly
higher than SLAnano and SLA.
conclusions : The strongest bone
response was achieved with a combina-
tion of wettability and the presence of
nanostructures (SLActive).
nAnostRuctuRes And hydRophilicity influence osseointegRAtion: A biomechAnicAl study in the RAbbit tibiA
9.
Nanostructures and hydrophilicity influence osseointegration: a
biomechanical study in the rabbit tibia. Wennerberg A, Jimbo R, Stübinger
S, Obrecht M, Dard M, Berner S. Clin Oral Implants Res. 2013
A head of print Jun 19
contact : [email protected]
figure 1. The prepared implant site in the rabbit tibia (left). The discs are covered with poly-tetrafluoroethylene caps and secured by a titanium band (right).
figure 2. Fixation of the bone during the tensile testing.
23
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Living organisms have developed (bio)
minerals with properties which at present
surpass those of their synthetic counter-
parts. There is still poor knowledge on
the mechanisms by which living organ-
isms control the growth and structure of
biominerals. Although there is an agree-
ment in that phosphorylated biomolecules
play a major role in regulating these
processes, it is still not clear whether this
regulation is exerted by molecules pre-
sent in the bulk or by those adsorbed on
biomineral surfaces and, in the latter case,
the nature of the molecule-mineral surface
interactions. To approach this problem,
we have investigated the interaction
between two model phosphoproteins (κ-
and β-casein) and hydroxyapatite (HAP),
i.e. the main building block in vertebrate
bones and teeth. Characterization of these
systems by means of ellipsometry and
QCM-D revealed that both phosphopro-
teins adsorb on HAP forming homogene-
ous elastic highly hydrated monolayers.
The mechanical stability of these films
was studied at the nanoscale by means
of an atomic force microscope operated
in the friction force spectroscopy mode.
This methodology provides the strength
of the adsorbed films in terms of the
forces needed for breaking and removing
them. The forces measured for β-casein
films were higher than those measured
for κ-casein by approximately a factor
four. Considering that β-casein possesses
five phosphorylated (SerP) residues and
κ-casein one, our data implies that both
caseins bind to the HAP surfaces through
their SerP sites and that the strength
of the films is critically dependent on
the SerP-Ca interaction. These results
may shed light e.g. on the mechanisms
by which phosphoproteins affect HAP
formation/dissolution.
on the oRigin of hydRoxyApAtite stAbilizAtion by phosphoRylAted biomolecules
10.
Sotres J., Barrantes A., Lindh L., Arnebrant T. 2014. Strategies for a direct characterization of phosphoproteins on hydroxyapatite surfaces. Caries Res. 48: 98-110.
contact : [email protected]
figure. Load vs Friction vs Roughness plots corresponding to the AFM-based scratching of β- and κ–casein films on HAp surfaces. Rupture and Removal events are indicated in both plots. AFM topographies, which show the scratched areas, are included in the upper-right corners of the plots.
24
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Sorption calorimetry is a very efficient
method to study hydration of biomol-
ecules and nanomaterials and it is used
in our laboratory in many projects. It has
although some limitations, in particular it
requires a high sample mass. Quartz crystal
microbalance with dissipation monitoring
(QCM-D) requires much lower sample
mass, but in hydration studies it typically
has a very low resolution since only a lim-
ited number of saturated salt solutions is
used. We introduced a new method for con-
tinuous measurements hydration processes
using QCM-D, where a non-saturated
solution of lithium chloride is used to set
up humidity of the air in contact with the
sample. During the experiment, the salt
solution is continuously diluted and the
relative humidity is continuously scanned
from about 11% up to almost 100%.
This technique was used to determine
the water sorption isotherm of lysozyme
films deposited on silica sensors as well
as to study rheological properties that
change during hydration-induced transi-
tions of the films. The sorption isotherm
obtained in QCM-D experiments is in
good agreement with sorption calorimetry
data. Analysis of the rheological behavior
during hydration of lysozyme films
revealed the presence of two transitions
at the water activities 0.67 and 0.91,
which are connected to the glass transi-
tion. Combination of the QCM-D data
and sorption calorimetric data allows
deeper understanding of glass transitions
in proteins. The new humidity scanning
QCM-D technique can be used for studies
of biomolecules available in tiny amounts.
development of humidity scAnning Qcm-d method
11.
Graf, G.; Kocherbitov, V. Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-
Resolution Humidity Scanning QCM-D Technique. J.Phys.Chem.B. (2013), 117,
10017−10026.
contact : [email protected]
figure. The experimental setup (left) and the results obtained on hydration of lysozyme (below)
25
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Over the past two decades, technolo-
gies such as chromatography and mass
spectrometry have driven the biochemi-
cal analyses of complex lipid mixtures,
tremendously advancing our knowledge of
lipid diversity. Lipid extraction (preceding
analysis), however, is still largely based on
partitioning procedures developed in the
1950s. Although appropriate for many
abundant components, these approaches
result in variable recovery of the less-
abundant and highly charged lipids,
including phosphorylated signaling lipids
whereof most biologically active lipids
contain at least one phospho-monoester,
for example, sphingosine-1-phosphate
(S1P). Long chain amino alcohols, gener-
ally referred to as long chain bases (LCB)
and their phosphorylated forms (LCB-P),
display particularly diverse chemistries
across biological species and tissues. In
human plasma, d18:1 sphingosine is the
most abundant LCB-P. However little is
known about the presence of other LCB-Ps
with different chemical and structural
composition despite the presence of vari-
ous non-phosphorylated LCB precursors.
In a collaboration led by National
University of Singapore, we have charac-
terized and quantified 10 LCB-P species
which were not described previously as
well as 6 LCB-P not known to be present
in the given biological samples tested here,
roughly doubling the number of known
LCB-P. This work has immediate relevance
for our understanding of S1P biology,
its therapeutic targeting and the LCB-P
signaling machinery which likely depends
on the precise chemical nature of their
aliphatic portion. The flow of sphingolipid
intermediates into sphingolipids (SPL) and
glycerophospholipids (GPL)/other lipids is
also of substantial general interest.
Thus, ‘deep profiling’ is a promising new
avenue in biochemical lipidomics for
the discovery of new bioactive lipids.
Crucial in this context is the integration
of synthetic capture phases developed
in Malmö designed to excert molecular
recognition of specific lipid motifs. Our
improved method is therefore expected
to have a pronounced impact in a variety
of fields.
‘deep pRofiling’ using enhAnced AnAlyticAl woRKflows ReveAls new sphingoid bAse-phosphAte species
12.
P. Narayanaswamy, S. Shinde, R. Sulc, R. Kraut, G. Staples, C. Thiam, R. Grimm, Börje Sellergren, F. Torta and M. R. Wenk; “Lipidomic “Deep profiling”: An enhanced workflow reveal new molecular species of signaling lipids” Anal. Chem. 2014.(Article ASAP) (DOI: 10.1021/ac4039652)
contact : [email protected]
26
Biofilms – Research Center for Biointerfaces
Annual Report 2013
LIST OF RESEARCH ACTIVITIESThe research activities of the Center during 2013 are presented under the headlines below.
In addition Center members are partners
in the eu fp7 Marie Curie Initial Training
Network “pepmip- Robust affinity mate-
rials for applications in proteomics and
diagnostics” (with Börje Sellergren as
Coordinator), eu fp7 Marie Curie Initial
Training Network “MagnIM” (Ann
Wennerberg), the eu fp7 Marie Curie
Initial Training Networks “Biofuel cells-
from fundamentals to applications in bio-
electrochemistry” (Sergey Shleev) and the
eu cooperation and mobility programme
Erasmus Mundus ember (Tautgirdas
Ruzgas).
27
Biofilms – Research Center for Biointerfaces
Annual Report 2013
cell-suRfAce inteRActions
Mechanism of Toxicity of Aluminium-
based Adjuvant (ABA) Nanomaterials
Funded by the British Medical Research
Council, MRC
pI: Håkan Eriksson
Analysis of the Cellular and Molecular
Mechanisms of Inflammatory Cells
and Cancer Cells by Using Digital
Holography and Protein Technology
PhD project funded by Mah,
Project partner: Phase Holographic
Imaging AB
pI: Anette gjörloff-wingren
Innovative Microscopy and Digital
Holography for Studies of Biomarkers
of Eukaryotic Cells Integrated to
Biosensors and Monitoring Devices
Post-doc project funded by BRCB
Project partner: Phase Holographic
Imaging AB
pI: Anette gjörloff-wingren
Biological Responses Nano-Size
Structures
Funded by the Knowledge Foundation
Project partner: Promimic
pI: Ann wennerberg
Hydrophilic and Hydrophobic Implant
Surfaces
Funded by industry
pI: Ann wennerberg
Advanced Surface Characterization
Funded by the Knowledge Foundation
Project partner: Halmstad University
and Ospol AB
pI: Ann wennerberg
Advanced Surface Characterisation of
New Functional Biomaterials
Funded by VR
Project partners: Chalmers and
Dentsply
pI: Ann wennerberg
1.
moleculAR tRAnspoRt phenomenA
Adjuvants for Products Used in
Agriculture
Project partners: AkzoNobel Surface
Chemistry AB
Funded by the Knowledge Foundation,
Mah
pI: Johan Engblom
Water – A Crucial Factor in Regulating
Biomembrane Permeability
Project partners: Physical Chemistry, LU
Funded by FLÄK, LU
pI: Emma Sparr (Lu), Johan Engblom
Humectants and Their Mechanisms
in Skin
Funded by the Knowledge Foundation
Project partners: SP AB, ACO Hud
Nordic AB, Eviderm Institute AB
pI: Johan Engblom
Mucoadhesion – A Prerequisite or a
Constraint in Nasal Drug Delivery
Funded by the Knowledge Foundation
Project partners: Nares AB, Bioglan AB,
LTH
pI: Johan Engblom
2.
28
Biofilms – Research Center for Biointerfaces
Annual Report 2013
3. moleculAR inteRActions At biointeRfAces
Hydration of Mucous Gel
Funded by Mah
pI: vitaly kocherbitov
Development of Novel Multi-Functional
Salivary Substitutes for Dry Mouth
Syndrome Patients
Funded by the Swedish Laryng
Foundation, Camurus AB, Mah
pI: Liselott Lindh
Screening of Different Surface Coatings
on Titanium including Different API’s
for Enhanced Wound Healing
Funded by the Swedish Laryng
Foundation and Mah
Project partner: NIOM, Oslo
pI: Liselott Lindh
Surface Modification with Natural
as well as Artificial Active Substances
of Dental Materials with the Aim to
Promote and Keep Good Oral Health
for the Spare Part Human
Funded by the Swedish Laryng
Foundation and Mah
Project partner: NIOM, Oslo
pI: Liselott Lindh
The Composition of Salivary Films
Formed on Surfaces with Different
Surface Wettability Including
Identification of Pellicle Proteins
Funded by the Swedish Laryng
Foundation and Mah
pI: Liselott Lindh
Carbohydrate Polymer - Water
Interactions: Sorption, Porosity and
Rheology
Funded by the Knowledge Foundation
Project partner: Magle AB, Akzo Nobel
Pulp and Performance Chemicals AB
pI: vitaly kocherbitov
Development of High-Resolution
Scanning QCM-D Method for Studies
of Hydration of Biofilms
Funded by BRCB
pI: vitaly kocherbitov
Hydration Effects in Condensed
Biopolymer Systems
Funded by SI
pI: vitaly kocherbitov
Properties of Semifluorinated Alkanes
Funded by Novaliq GmbH
Project partner: Novaliq GmbH
pIs: Johan Engblom, vitaly kocherbitov
Interfacial Behaviour and Activity of
Laccase and Bilirubin Oxidase on Bare
Gold Surfaces
Funded by Mah
pI: Sergey Shleev
Viscoelastic Bioelectrocatalytic
Materials
Funded by VR
pIs: Tautgirdas Ruzgas, Thomas Arnebrant
Devices for Non-Invasive Biomedical
Sensing, Monitoring or/and Delivery
Funded by the Knowledge Foundation
Project Partners: Galenica AB,
Novosense, Speximo AB, Bioglan AB
pI: Tautgirdas Ruzgas
Flexible and Transparent Biofuel Cells
Funded by EU, Marie Curie
Project Partner: Obducat
Technologies AB
pI: Sergey Shleev
29
Biofilms – Research Center for Biointerfaces
Annual Report 2013
micRobiAl biofilms
Oral Implants – Nanostructures for the
Promotion of Tissue Integration and
Prevention of Infections
Funded by the Knowledge Foundation
Project partners: Noble Biocare AB,
Promimic AB
pIs: gunnel Svensäter, Ann wennerberg
Bacterial Acid Tolerance – a New
Target for Fluoridated Milk in Caries
Prevention
Funded by the Borrow Foundation, UK
pIs: gunnel Svensäter, Julia Davies
Effect of surface characteristics on
cellular adherence and activity
PhD project, National Graduate
Research School in Odontology, funded
by Vetenskapsrådet, Mah
pIs: Marjan Dorkhan, Julia Davies, gunnel Svensäter
Biomarkers for infection
control on the biofilm level
Funded by Mah
pIs: gunnel Svennsäter, Julia Davies, Claes wickström, Jessica Neilands
Mucins and microbial biofilms
– a symbiotic relationship for health
Funded by Mah
pIs: gunnel Svennsäter, Julia Davies,
Claes wickström
The effect of probiotic lactobacilli on
development of acid adaptation in
dental biofilms
Funded by the Knowledge Foundation
pI: Jessica Neilands
Mechanisms underlying phenotypic
adaptation in Streptococci
Funded by Mah
pIs: gunnel Svennsäter, Julia Davies
Protease inhibitors for a balanced
proteolytic activity around teeth
Funded by Mah
pIs: Bertil kinnby, Jessica Neilands
4.
Restorable and Adaptable Surfaces for
Molecular Recognition, Biosensing
and Evanescent Wave Microscopy of
Human Cells
Funded by VR
pI: Börje Sellergren
Caffeine Scavenger
Funded by Biotage AB
Project partner: Biotage AB
pI: Börje Sellergren
30
Biofilms – Research Center for Biointerfaces
Annual Report 2013
SCIENTIFIC INSTRUMENTATION AND EqUIpMENT
opticAl micRoscopy
Contact person: peter FalkmanA Nikon Optiphot Epi-Fluorescence
microscope equipped with polarizing
filters, phase contrast and a DS-U1
digital camera. A TransferMan NK2
micro manipulator allows manipulation
of samples, such as mounting of AFM
colloidal probes, and a Linkam Analysa-
LTS350 temperature controlled stage
allows study of temperature-induced
effects, e.g. lipid phase transitions.
1.
31
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Contact person: Johan Engblom Three alternative thermostated in vitro dif-
fusion equipments are available in house,
two set ups with flow through cells (15 +
7 cells) [R.L. Bronaugh & R.F. Stewart, J.
Pharm. Sci. 74 (1985) 64–67] and 10 static
Franz cells [Franz T, J. Invest. Dermatol.
64 (1975) 190-195]. Solute diffusion over
skin, oral mucosa, nasal mucosa, nails, plant
cuticle, as well as synthetic membranes,
has been studied and assayed spectropho-
tometrically (on-line) or by HPLC-UV.
Electrical impedance spectroscopy has
been appended to the Franz cells as a tool
helping to explain diffusion properties of
solutes through these membranes.
in vitRo diffusion eQuipment
swAxd
Contact persons: peter Falkman,
Johan EngblomSmall and Wide Angle X-Ray Scattering.
Kratky compact camera with line col-
limation (slit focus), equipped with two
linear MBraun detectors (small and wide
angle). Temperature controlled sample
stages (0–70°C or 25–300°C). Powder
(semi-solid/solid) or capillary (liquid)
sample holders, as well as flow-through
capillary sample holder.
dsc
Contact person: vitaly kocherbitovDifferential scanning calorimeter DSC1
from MettlerToledo equipped with an
intracooler TC100 and HSS8 sensor.
Temperature range: –90 to +550°C. The
calorimeter can be used to study solid,
soft and liquid substances. Typical sample
masses are 2–10 mg. The DSC can be run
in modulation regime to resolve reversing
and non-reversing heat effects.
5.
2.
3.
4.
ellipsometeR
Contact persons: peter Falkman,
Thomas ArnebrantAn automated Rudolph thin film ellipso-
meter (type 43603-200E, Rudolph
Research, USA) equipped with a xenon
arc lamp as a light source; an interference
filter with UV and infrared blocking (Melles
Griot, The Netherlands) for working at a
wavelength of 4429 Å. The experimental
setup is based on null ellipsometry in the
PCSA arrangement. The components of
the ellipsometer are controlled by means
of the Ellipso software that automatically
measures the ellipsometric angles Psi and
Delta allowing the calculation of the thick-
ness, refractive index, and adsorbed mass of
the growing films. To perform experiments
in liquid media a peristaltic pump (Ole Dich
Instruments) is used. The temperature is
controlled by means of a Julabo 5B water
bath. Data analysis is performed with the
Ellipsometry software by Plamen Petrov.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
Qcm-d
Contact person: Sebastian BjörklundThe Q-Sense E4 quartz crystal micro-
balance is equipped with the E4 measuring
chamber that allows simultaneously run-
ning a maximum of four experiments in the
four temperature controlled cells. Changes
in frequency and dissipation are registered
by means of the E-series electronic unit
which is also responsible for the tempera-
ture control. These changes are related to
the adsorbed wet mass and the viscoelastic
properties of the films adsorbed onto the
sensor. The QCM-D is also equipped with
an electrochemistry and an ellipsometry
module enabling the simultaneous meas-
urement of the electrochemical and optical
properties respectively of the adsorbed
films. A four channels IPC peristaltic pump
(Ismatec). Data analysis is performed with
the Q-Tools software (Q-Sense).
7.
6.
8.
e-sem
Contact person: peter Falkman, Zoltan BlumScanning Electron Microscope, SEM,
Zeiss EVO LS10. Apart from standard
modern SEM features, i.e. 5 nm point-
to-point resolution at high vacuum and
fully conducting samples, the EVO LS10
also offers environmental control (hence
“EVO”). By controlled water vapor intro-
duction, with or without the added ben-
efits of a Peltier Coolstage, fully hydrated
samples can be imaged with accurate
sample temperature control; owing to the
high output LaB6 filament and the beam
sleeve technology, the loss in resolution is
only tenfold. The instrument is equipped
with the appropriate detectors and also an
INCA EDX microanalysis system.
spectRoscopic ellipsometeR uvisel hoRibA
Contact person: Marité CardenasSpectroscopic ellipsometry is a simple
and fast optical and indirect technique
that offers the possibility to determine the
average thickness of the adsorbed layer.
Ellipsometry measures the ratio of two
values, which is the amplitude ratio (Ψ=rp/
rs) and the phase difference between light
waves (Δ=Δp-Δs). These two parameters
are defined from the difference in the reflec-
tion coefficients for p- and s-polarizations
and thus in the optical properties of a
material (extinction co efficient and refrac-
tive index). We have in house an UVISEL
HORIBA spectroscopic ellipsometer cover-
ing a wavelength range of 200 – 820 nm.
33
Biofilms – Research Center for Biointerfaces
Annual Report 2013
dls/zetA sizing system
Contact person: peter FalkmanA combined Electrophoretic Light
Scattering/Dynamic Light Scattering Zeta
potential/particle sizing system with a 5
MW 632.8 nm HeNe laser, two insert-
able electrodes for high- or low voltage,
covering possible electrical field strengths
between 0.25–250 V/cm, and an adjust-
able temperature range of 4–100°C. The
instrument has further been fitted with a
Thermaltake Bigwater 760is liquid cool-
ing system, to prevent cuvette fogging at
high temperatures.
goniometeR
Contact person: peter FalkmanKruss Goniometer DSA100 Drop shape
analyzer system for measurements of static
and dynamic contact angles. The gonio-
meter is equipped with single dosing system
DS3200/3201. Temperature is controlled
in the range 5–90 °C using TC30 measur-
ing chamber. Humidity is controlled using
HC10 humidity chamber.
Afm
Contact person: Javier Sotres,
Thomas ArnebrantA Multimode SPM equipped with a
Nanoscope VIII control unit (Bruker AXS).
The SPM is also equipped with a PicoForce
Unit, a Heater Unit, and the ScanAsyst
and Peak Force Tapping softwares (Bruker
AXS). Furthermore, a digital oscilloscope
(Tektronix TDS 2022C) and an Nikon opti-
cal microscope are coupled to the system.
impedAnce meAsuRing eQuipment
Contact person: Tautgirdas RuzgasFranz cells (Ø=0.90 cm, V=6 ml, PermeGear,
Hellertown, PA, USA) equipped with four
electrodes and connected to a potentiostat
from Ivium Technologies (Eindhoven, The
Netherlands) are used to study impedance
characteristics of membranes, including
skin, leafs, etc. Two platinum wires serve
as working and counter electrodes and two
Ag/AgCl/3M KCl electrodes from World
Precision Instruments (Sarasota, FL, USA)
are used as sensing and reference electrodes.
The equipment allows impedance measure-
ments at frequencies up to 6 MHz.
11.
9.
10.
12.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
RheometeR
Contact person: vitaly kocherbitov,
peter FalkmanRheometer Bohlin CVO 100 Digital
equipped with Peltier Cylinder C25. The
rheometer system is suitable for research,
product development and quality control.
The rheometer is equipped with 20 mm
parallel plate, cone 4/40 and coaxial cyl-
inders measuring systems.
soRption cAloRimeteR
Contact person: vitaly kocherbitovA double-twin sorption calorimeter for
studies of hydration of powders and soft
materials. The channel diameter is 28 mm.
The calorimeter is equipped with two sorp-
tion cells, each consist of a sorption cham-
ber (on top) and a vaporization chamber
(bottom). The cambers are connected by
tubes with varying diameters to control the
vapor flow. The double twin calorimeter
is inserted into an isothermal TAM 2277
calorimeter from Thermometric equipped
with a nanoamplifier.
pARticle electRophoResis
Contact person: Tautgirdas RuzgasParticle micro-electrophoresis apparatus
Mark II (Rank Brothers, Cambridge,
UK) consists of flat electrophoretic cell
equipped with two platinum electrodes
and optical microscope. The apparatus can
be used to determine particle electropho-
retic mobility in solution. The mobility can
be used to assess particle zeta potential and
surface charge density. Apparatus allows
measurements with particles bigger than
0.3 µm in diameter.
lAngmuiR suRfAce bAlAnce
Contact person: Johan Engblom,
Marité CardenasKSV 5000 is a programmable Langmuir
and Langmuir-Blodgett instrument for
automated Langmuir film experiments and
for deposition of normal or alternating
multilayers onto solid substrates. Surface
pressure is measured using the Wilhelmy
plate method.
dRop volume AppARAtus
Contact person: Tautgirdas RuzgasDrop Volume Tensiometer TVT 2 consists
of step motor driven syringe including a
needle for droplet formation, setup for
optical droplet falling registration, and
software to operate hardware and calculate
surface tension of liquid. This apparatus is
used for determination of surface tension
of liquids.
14.
13.
15.
16.
17.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
inteRfeRometeR
Contact persons: Ryo Jimbo, Ann wennerbergWhite light microscopy, horizontal reso-
lution 0.3 µm, vertical 0.1 nm, equipped
with a CCD camera, operating also in
phaseshift mode. The main application is
topographical characterisation on the µm
and sub-µm level. AFM Park Instrument,
surface characterization in the nm level.
Real time PCR- Gene expression
novAsinA lAbmAsteR-Aw
Contact person: Johan EngblomAn instrument for water activity measure-
ments. Accuracy: 0.003 aw (0.3%RH),
range: 3 to 100%RH.
lc-ms
Contact person: Börje SellergrenZQ2000 MS system with 2795 LC and
2996 PDA. The Waters ZQ 2000 LCMS
System is based on the Micromass-ZQ 2000
Mass Selective Detector. This is a robust sin-
gle quadrupole mass spectrometer, offering
characteristic performance for laboratories
that require nominal mass resolution for
mass confirmation applications. This system
has a mass range (amu) up to 2000 daltons.
Easily qualify and quantify small molecules
in a flash with the highly sensitive system.
Both inlet probes are quickly and easily
interchangeable without breaking vacuum.
Flow rates allowed are 5–1000 µl/min (ESI)
and 200–2000 µl/min (APCI).
lc -uv/dAd
Contact person: Börje SellergrenTwo Waters LC-UV/DAD 2795/2996
(LCDAD) systems. This robust HPLC
apparatus allows flow-rates from 50ul/
min to 5ml/min for use with 2.1 mm ID
columns and larger. The autosampler
accepts standard 96/384 well plates with
programmable temperature control from
4 to 40°C.A heated column compartment
provides temperatures from 5 degrees
above ambient to 65°C. A range of LC
detectors, like a dual wavelength- or PDA
UV detector is optionally available.
fluoRchem e
Contact person: Anette gjörloff wingrenHigh-performance Western blot and gel
imaging. A self-contained digital dark-
room, compact design with state-of-the-art
CCD optical technology, an integrated
computer and simplified touch screen
control. The FluorChem E is built with
imaging speeds and a dynamic range that
surpass film for low femtogram detection
of proteins and DNA in chemiluminescent,
colorimetric and UV fluorescent gels and
blots.
20.
18.
19.
21.
22.
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
biofilm foRmAtion, including AdheRence, detAchment, populAtion diveRsity And phenotypic shift
Contact person: gunnel SvensäterEpifluorescence microscope
Confocal laser scanning microscope
Flow cells with controlled continuous flow
Cell cultures
puRificAtion of mucins And pRoteins
Contact person: gunnel SvensäterFPLC
Preparative electrophores
Ultracentrifuge
pRoteomics, including RAdioActive lAbelling of pRoteins
Contact person: gunnel SvensäterMultiphor
Two-dimensional electrophoresis
Sonicator
identificAtion of pRoteins
Contact person: gunnel SvensäterImmunohistochemistry
Western blots
ELISA
electRochemicAl eQuipment
Contact person: Sergey Shleev6 potentiostats/galvanostats from Autolab,
BAS, and Ivium. Unique spectroelec-
trochemical system (home-made 10 L
spectroelectrochemical cell, spectrometer
HR 4000 CG-UV-NIR and light source
DH-2000 from Ocean Optics). Flow-
injection analysis
micRobiAl diAgnostics; species And stRAin levels
Contact person: gunnel SvensäterAutoclaves
Anaerobic chamber
Fluorostar
Incubators
Spectrophotometer
PCR
Centrifuges
24.
23.
25.
26.
27.
28.
37
Biofilms – Research Center for Biointerfaces
Annual Report 2013
COLLABORATIVE pARTNERS OF THE CENTER
industRy collAboRAtoRs
ACO Hud Nordic AB
Acreo AB
Akzo Nobel Pulp and Performance
Chemicals AB
Akzo Nobel Surface Chemistry AB
Bioglan AB
Biotage AB
Camurus AB
CapSenze HB
Corigo AB
Dentsply
Dräger AG, Lübeck, DE
Enzymatica AB
Eviderm AB
Galenica AB
GT Septech, Oslo, NO
In vitro Plant-tech AB
Indienz AB
Magle AB
Medeon AB
Medicon Valley Alliance
Nares AB
Nobel Biocare AB
Novaliq GmbH, DE
Novosense AB
N-Zyme Biotech, Darmstadt, DE
Obducat Technologies AB
Orbital Systems AB
Promimic AB
SP AB
Speximo AB
Thermo Fischer, UK
Zelmic AB
1.
38
Biofilms – Research Center for Biointerfaces
Annual Report 2013
AcAdemic collAboRAtoRs
collAboRAtions with otheR univeRsities And ReseARch institutions
in sweden
Professor Anders Rosén, Cell Biology, university of Linköping, Linköping
Professor Artur Schmidtchen, Lund university
Professor Chris Anderson, Dermatology, university of Linköping, Linköping
Professor em. Kåre Larsson, Camurus Lipid Research Foundation, Lund
Professor Emma Sparr, physical Chemistry 1, Lund university
Professor Fredrik Tiberg, Camurus AB, Lund
Professor Gunnar Dahlén, Dept of Oral Microbiology, university of gothenburg
Professor Ian Nicholls, Lineaus university, kalmar
Professor Jens Schouenborg, Neuronano Research Center, Medical Faculty, Lund university
Professor Knut Irgum, uME, umeå
Professor Kristian Reisbeck, Dept of Medical Microbiology, Lund university
Professor Lars Montelius, Solid State physics, Lund university
Professor Lo Gorton, Biochemistry, Lund university
Professor Marie Wahlgren, Food Technology, LTH, Lund university
Professor Mark Rutland, The Royal Institute of Technology and Sp AB, Stockholm
Professor Martin Andersson, Dept Applied Chemistry, Chalmers university of Technology
Professor Martin Malmsten, pharmacy, uppsala university
Professor Maud Langton, Swedish university of Agricultural Sciences, uppsala
Professor Olle Söderman, physical Chemistry, Lund university
Professor Pentti Tengvall, Dept Biomaterials, Sahlgrenska Academy, göteborg university
Professor Per Claesson, The Royal Institute of Technology and Sp, Stockholm
Professor Per Wollmer, Clinical Sciences, Lund university
Professor Staffan Nilsson, Lund university
Professor Thomas Laurell, Lund university
Professor Tomas Albrektsson, Dept Biomaterials, Sahlgrenska Academy, göteborg university
2.
39
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Professor Tommy Nylander, physical Chemistry, Lund university
Professor Viveka Alfredsson, physical Chemistry, Lund university
Assoc. Professor Malin Sjöö, Food Technology, Lund university
Assoc. Professor Marilyn Rayner, Food Technology, Lund university
Assoc. Professor Ola Bergendorff, Dermatology, Lund university
Assoc. Professor Eva Blomberg, The Royal Institute of Technology and Sp AB, Stockholm
Assoc.Professor Peter Siesjö, Department of Clinical Sciences, BMC, Lund university
Per Rabe, Specialist Clinic for Oral Health Care, Hallands Läns Landsting, Halmstad
Doctor Andreas Sonesson, Lund university
Doctor Anna Westerlund, Odontology, gothenburg university
Doctor Annika Krona, The Swedish Institute for Food and Biotechnology, gothenburg
Doctor Birgit Brandner, Sp kM AB, Stockholm
Doctor Christer Wingren, LTH, Lund university
Doctor Jenny Liao Persson, Center for Molecular pathology, Lund university
Doctor Karina Persson, Inst. of Odontology, umeå university
Doctor Maria Lövenklev, The Swedish Institute for Food and Biotechnology, gothenburg
Doctor Marie Skepö, Dept of Theoretical Chemistry, Lund university
Doctor Robert Corkery, The Royal Institute of Technology
Doctor Björn Svensson, ÖTL, Specialist docent, Clinic for Oral Health Care, Örebro Läns Landsting, Örebro
Doctor Sylvio Haas, MAX, Lund university
Doctor Tomás Plivelic, MAX lab, Lund university
Brånemark Clinic, gothenburg
40
Biofilms – Research Center for Biointerfaces
Annual Report 2013
inteRnAtionAl collAboRAtions
Professor Anja Boisen and post-doc Robert Burger, DTu Nanotech, Copenhagen
Professor Ben J. Boyd, Monash Institute of pharmaceutical Sciences, Monash university, Australia
Professor Christopher Exley, keele university, uk
Professor David Beighton, guy’s kings and St Thomas’ Dental Institute, Joint Microbiology Research unit, London, uk
Professor Deirdre Devine, Leeds Dental Institute, uk
Professor Dennis Cvitkovitch, Dept of Microbiology, university of Toronto, Canada
Professor Dietmar Haltrich, universität für Bodenkultur wien, Austria
Professor Doctor Alexander Yaropolov, Institute of Biochemistry, Moscow, Russia
Professor ECI Veerman, Department of periodontology and Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA)
Professor Edmond Magner, university of Limerick, Ireland
Professor Gediminas Niaura, vilnius university, Lithuania
Professor Gordon Proctor, kings College, London, uk
Professor Janusz Pawlitzyn, university of waterloo, Canada
Professor Jeannine Brady, Dept of Oral Biology, university of Florida, gainesville, uSA
Professor Jens Wiltfang, university of Essen, germany
Professor Jon E. Dahl, NIOM, Oslo, Norway
Professor Jukka Meurman, Dept of Oral and Maxillofacial Diseases, Helsinki university Central Hospital, Helsinki, Finland
Professor Juozas Kulys, Inst. of Biochemistry, vilnius, Lithuania
Professor Kamal Mustafa, university of Bergen, Norway
Professor Katrin Markus, university of Bochum, germany
Professor Leon Rubesaet, uIO, Oslo, Norway
Professor Mark Herzberg, Department of Microbiology, university of Minnesota, Minneapolis, uSA
Professor Markus Wenk, National university of Singapore, Singapore
Professor Ole Jensen, SDu, Odense, Denmark
Professor Peter Cormack, university of Strathclyde, uk
41
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Professor Phil Bartlett, The university of Southampton, uk
Professor Phil Marsh, Health protection Agency, porton Down, uk
Professor Robert Baier, university of Buffalo, Industry/university Cooperative Research Center for Biosurfaces, Buffalo, uSA
Professor Takashe Sawase, Dept prosthodontics, university of Nagasaki, Japan
Professor Vladimir O. Popov, kurchatov NBIC Centre, Moscow, 123182, Russia
Professor Wolfgang Schuhmann, Ruhr-universität Bochum, germany
Assoc. Professor David Thornton, university of Manchester, uk
Assoc. Professor Marie Ranson, School of Biological Sciences, Scientific Director – Cancer, Illawarra Health and Medical Research Institute, university of wollongong, wollongong, Australia
Doctor Anne Meyer, university of Buffalo, Industry/university Cooperative Research Center for Biosurfaces, Buffalo, uSA
Doctor Antonio L. De Lacey, Consejo Superior de Investigaciones Científicas, Bioelectrocatalysis group, Spain
Doctor Donald Leech, National university of Ireland, galway, Ireland
Doctor Humberto Schwartz, Sao paolo, Brasilien
Doctor Jan-Tore Samuelsen, NIOM, Oslo, Norway
Doctor Jelena Kosoric, Centre for Oral growth and Development, queen Mary’s School of Medicine and Dentistry, university of London, uk
Doctor Miguel Alcalde, Consejo Superior de Investigaciones Científicas, Applied Biocatalysis group, Spain
Doctor Paulo G. Coelho, Department of Biomimetics, New york university, uSA
Doctor Sergei Lobov, Browitt Nanoparticle Lab, Dept of Applied Mathematics, Australian National university, Canberra, Australia
Doctor Yuusuke Kozai, Department of Radiology, kanagawa Dental university
Assoc. Professor Tim Tolker-Nielsen, university of Copenhagen, Denmark
PhD student Nayab Chaudhury, kings College, London, uk
National Research Centre kurchatov Institute, Moscow, Russia
42
Biofilms – Research Center for Biointerfaces
Annual Report 2013
wORkSHOpS AND CONFERENCES ORgANISED BY THE CENTER
The Center launched the 9th Annual Workshop in October. The focus this year was ‘Biomarkers’ and the event attracted 90 participants from different universities as well as industry. Read more about the workshop in the article below.
Claes Wickström and Jessica Neiland
were responsible for organising the 8th
International Conference on Problem-
Based Learning in Dentistry, which was
held at the Faculty of Odontology in
May. The Faculty of Odontology has been
working with problem-based learning since
the early 1990s and this was the second
time that the Faculty has organised the
conference. The conference attracted 91
participants from 19 countries.
The Topical Science Cluster – a joint
venture between the Center and LPR@
LU – Pharmaceutical Research at Lund
University – organised three well-received
workshops during 2013.
• “A meeting between clinical research
and technology around our shared
interface: the skin”, held at the
Department of Dermatology and
Venereology, Skåne University
Hospital
• “Human Interfaces: Structure and
transport in skin and in lung alveoli”,
held at the Department of Physical
Chemistry, Lund University
• “From Molecule to Man!” held at
ASA Herrgård, Lammhult.
The three workshops attracted both
national and international speakers.
Ann Wennerberg and Gunnel Svensäter,
who are coordinators for the National
Graduate Research Schools in Odontology,
organised the summer conference
“Approaches to clinical research”, which
took place at Ystad Saltsjöbaden in June.
Börje Sellergren, who is coordinating
the EU FP7 Marie Curie Initial Training
Network PEPMIP – Robust affinity mate-
rials for applications in proteomics and
diagnostics, organised the business devel-
opment seminar “From idea to product”
together with Biotage in Lund as part of
training activities in the network. Börje
has also been involved in arranging the
following:
• The Graduate Student Symposium
at Belfast University, which attracted
44 participants and included lectures
on Molecular Imprinting Technology
delivered by graduate students.
• The workshop “Monolithic Materials
and Synthesis and Characterisation
of Porous Materials”, held at Umeå
University. The event attracted 36
participants.
• The 5th International HILIC Day,
held at Umeå University. The meeting
attracted 40 participants.
Also worth mentioning is Johan
Engblom’s participation in the Tv pro-
gramme “vetenskap” with Madeleine
westin on Tv4 play
http://www.tv4play.se/program/
vetenskap?video_id=226369.
43
Biofilms – Research Center for Biointerfaces
Annual Report 2013
biomARKeRs – 9th AnnuAl woRKshop of bRcb
The annual workshop was held on
October 3–4 and addressed technical
aspects and applications of biomarkers.
Some 80 participants from academia and
industry took part.
“I’m extremely interested in research and
this is the third workshop in a row that I
have attended,” says Helena Ekdahl from
Labjoy AB.
The workshop, the ninth in succession,
focused on techniques for analysing
biomarkers.
The speakers included Dr Sarah Fredriks-
son, President of Genovis AB, who spoke
about nanostructures that can improve
medical image analysis, such as MRI. She
emphasised how important the design of a
nanostructure is to the end result. Professor
György Marko-Varga, head of the Division
of Clinical Protein Science and Imaging at
Lund University and the person respon-
sible for the development of biomarkers
in the Skåne Region, spoke about the
LUNDHEARTGENE project. This is a
large biobank with patient samples and
György spoke about how, based on this,
an analysis has been made of cardiovas-
cular markers in blood plasma. The aim
is to try to find new proteins as a goal in
pharmaceutical development.
Professor Börje Sellergren at BRCB at
Malmö University, spoke about molecular
imprinting. In simple terms, this involves
making imprints in plastic that can then
bind molecules, similar to the way anti-
bodies function. Associate Professor Asli
Silahtaroglu from Copenhagen University
spoke about microRNAs, which are short
RNA strands that control different gene
expressions. Asli spoke about being able
to detect specifically microRNAs in tissue
sections. Many microRNAs function as
diagnostic markers for different diseases,
such as cancer. Circulating microRNAs
are also a new potential generation of
biomarkers that can be used to discover
heart diseases.
One of the participants, Helena Ekdahl
from Labjoy AB, named Trippelhelix, the
new innovation model for collaboration
between academia, industry and the public
sector, and stated that it would be very
much a win-win situation if everyone is
linked together.
The workshop offered opportunities to
create new contacts. “This year’s work-
shop has been extremely interesting and
taking part has been a good way to keep
up to date on research,” Helena concludes.
1.
44
Biofilms – Research Center for Biointerfaces
Annual Report 2013
SEMINARS AT THE CENTER
Naghi Momeni, Medeon, Malmö, “Autism Spectrum Disorder (ASD)”
Nina Oestergaard Knudsen, LEO Pharma, “Lipid nanoparticles for treatment of skin diseases with barrier impairment”
Petri Gudmundsson, Mah, “Pedagogic model – constructive alignment”
Marius Dagys, Institute of Biochemistry, Vilnius “New oxidoreductases for development of biofuel cells”
Jonas Carlstedt, Mah, “Hydration of starch: sorption, glass transition and structure”
Börje Sellergren, Mah, “Molecularly imprinted polymers”
Hala Gari, Mah, “Characterisation of biological membranes by impedance spectroscopy”
Gesche Graf, Mah, “Development of a high-resolution scanning QCM-D technique”
Charlotta Turner, Lund University, “Department of Chemistry, Green Technology Group”
Patrick Seumo, University of Yaounde I, Yaounde, Cameroon, “Electrochemical quantifi-cation of protein concentration”
Ben J. Boyd, Monash University, Australia, “Controlling structural transformation in self assembled lipid liquid crystals for drug delivery”
Ausra Linkeviciute, Center for Physical Science and Technology “Food and drugs: different roles of nanotechnology”
Per Hyldgaard, Microtechnology and Nanoscience, MC2, Chalmers, “A parameter-free computational theory of biomolecular interactions”
Thomas Zemb, Institute for Separation Chemistry of Marcoule, France, “Surfactant-free microemulsions”
Peter Spégel, Unit of Molecular Metabolism, Lund University Diabetes Centre, “Metabolomics in medical research”
Gordon Proctor, Kings College, UK, “Comparison of saliva from dry mouth patients with saliva from healthy individuals”
Nayab Chaudhury, Kings College, UK “Comparison of saliva from dry mouth patients with saliva from healthy individuals”
Lars-Åke Johansson, Halmstad “On minimally invasive approaches to sinus lift procedures”
45
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Maria Pigg, Mah, “When should we save the tooth and when is an implant the better solution?”
Lory Melin-Svanborg and Nils Lövgren, Mah, “How shall we evaluate endodontically treated teeth at prosthetic therapy planning?”
Camilla Ahlgren, Mah, “Material reactions”
Rolf Liedholm, Mah, “Mucus membrane changes due to prosthetic treatments”
Barbro Swartz, Örebro University, ”Juvenile Prosthodontics”
Per Vult von Steyern, Mah, “Ceramics”
Gunilla Nordin Fredrikson, Faculty of Medicine, Lund University, “Immune biomarkers
Karin von Wachenfeldt, Truly Translational AB, “Design of clinical studies for biomarker evaluation”
Thuy Tran, Bioimagning Center, Lund University, “In Vivo Detection of Biomarkers using PET/SPECT/CT-imaging modalities”
Sarah Fredriksson, Genovis AB, “Using Nanostructures as Multimodal Contrast Agents in Medical Imaging”
György Marko-Varga, Clinical Protein Science & Imaging, Lund University, “Biomarker Diagnosis by Protein Sequence assay Develoments utilizing Mass Spectrometry”
Börje Sellergren, Faculty of Health and Society, Malmö University, “Molecularly imprinted polymers in biomarker analysis”
Ali Moazzami, Metabolomics, SLU, “Metabolomics a tool to identify the biomarkers of physiological status”
Malin Lindstedt, Immunotechnology, Lund University, “Signature biomarker analy-sis for prediction of skin sensitizers using a cell- based in vitro alternative to animal experimentation”
Asli Silahtaroglu, Faculty of Health Sciences, University of Copenhagen, “In situ detection of members of a microRNA family using novel probe chemistries”
Joyce Carlson, MD, Clinical Chemistry and Pharmacology, Lund University, “Biobanking for Biomarkers”
Simon Ekström, Electrical Measurements, Lund University, “Microfluidic sample handling for Biomarker Analysis”
Bo Åkerström, Faculty of Medicine, Lund University, “Prediction and diagnosis of preeclampsia”
Maria Falck Miniotis, Faculty of Health and Society, Mah, “Digital holographic micros-copy for non-invasive monitoring of cellular responses to cancer therapy”
46
Biofilms – Research Center for Biointerfaces
Annual Report 2013
pHD THESES SUpERVISED BY CENTER MEMBERS
theses defended: Alma MašićLund university (Supervisors: per Ståhle,
Johan Helsing)
“Investigation of a biofilm reactor model
with suspended biomass”
Dissertation 2013-03-22
Marija Jankunec
vilnius university (Supervisor: Justas
Barauskas)
”Investigation of properties of lipid
liquid crystalline drug carriers and their
interactions with model cell membranes”
Dissertation 2013-06-06
Sebastian Björklund
Lund university (Supervisors: Emma Sparr at
Lu, Johan Engblom Mah, krister Thuresson
at Camurus AB)
“Skin hydration – How water and osmolytes
influence biophysical properties of stratum
corneum”
Dissertation 2013-06-14
Yana Znamenskaya
Malmö university (Supervisors: vitaly
kocherbitov, Johan Engblom)
“Effect of hydration on thermodynamic, rheological and structural properties of mucin” Dissertation 2013-09-12
theses in pRogRess: Anton Fagerström
Malmö university (Supervisors: Johan
Engblom, vitaly kocherbitov, karin
Bergström at Akzo Nobel Surface Chemistry
AB)
“Bioavailability of active ingredients used in
agriculture”
Start: 2009
Marjan Dorkhan
Malmö university (Supervisors: Julia Davies,
gunnel Svensäter)
“Activities of microbial biofilms on bioactive
implant surfaces”
Start: 2009
Magnus Falk
Malmö university (Supervisors: Sergey
Shleev, Tautgirdas Ruzgas)
“Direct electron transfer based biofuel cells”
Start: 2009
Tuerdi Maymaytilli
LTH (Supervisors: per Ståhle, Christina
Bjerkén)
“Influence of plastic deformation on the
formation and growth of embritteling metal
hydride’s”
Start: 2009
Liselott Ellmarker-Löfquist
Malmö university (Supervisors: Liselott
Lindh, Mats kronström,)
”A Clinical and subjective evaluation of
single implant treatments. A retroscpective
study.”
Start: 2009
Mariko Hayashi, Malmö University
(Supervisors: Liselott Lindh, Ann
wennerberg, Ryo Jimbo)
“Biological Responses to Amphiphilic Nano-
size Structures”
Start: 2010
47
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Peter Lamberg
Malmö university (Supervisors: Tautgirdas
Ruzgas, Thomas Arnebrant)
“Three-dimensional (3D) bioelectrochemical
systems assembled from nanoparticles”
Start: 2010
Cathrine Albér
Malmö university (Supervisors: Johan
Engblom, vitaly kocherbitov, Marie Lodén,
Robert Corkery)
“Humectants and their effects on skin barrier
function – a mechanistic approach”
Start: 2010
Zahra El-Schich
Malmö university (Supervisors: Anette
gjörloff wingren, Anna ketelsen, gunilla
Nordin Fredrikson)
“Microscopy and digital holography for
real-time applications and imaging: studying
the cellular and molecular mechanisms of
inflammatory cells and cancer cells”
Start: 2011
Celina Wierzbicka
Malmö university (Supervisor: Börje
Sellergren, Thomas Arnebrant):
“New approaches to molecularly imprinted
micro- and nano- particles for posttrans-
lationally modified proteins and protein
fragments”
Start: 2012
Dmitrii Pankratov
Malmö university (Supervisors: Sergey
Shleev and Zoltan Blum)
“Function of bioelectronic devices in “extra
vivo” situations”
Start: 2012
Mark Galat
Malmö university (Supervisor: Börje
Sellergren, Thomas Arnebrant)
“Development and characterisation of
molecularly imprinted polymer receptors tar-
geting pathogenic and biomarker peptides
related to neurodegenerative diseases”
Start: 2013
Sing Yee Yeung
Malmö university (Supervisors: Börje
Sellergren, Thomas Arnebrant)
“Restorable and adaptable surfaces for
molecular recognition, biosensing and eva-
nescent wave microscopy of human cells”
Start: 2013
Pär Johansson
Malmö university (Supervisors: Ann
wennerberg, Ryo Jimbo)
“A unique surface modification to improve
bioactivity of a polymer, pEEk, for ortho-
paedic and dental applications”
Start: 2012
Silvia Galli
Malmö university (Supervisors: Ryo Jimbo,
Ann wennerberg)
“On the effect of magnesium incorporated
biomaterials for bone regeneration”
Start: 2012
Francesca Cecchinato
Malmö university (Supervisors: Ryo Jimbo,
Ann wennerberg)
“Characterisation of magnesium alloys for
oral & maxillofacial applications”
Start: 2012
Ramesh Chowdhary
Malmö university (Supervisors: Ann
wennerberg, Liselott Lind, Lennart Carlsson)
“On efficacy of implant thread design for
bone stimulation”
Start: 2009
48
Biofilms – Research Center for Biointerfaces
Annual Report 2013
Jonas Anderud
Malmö university (Supervisors: Ann
wennerberg, Sten Isaksson, peter
Abrahamsson)
“guided bone regeneration using ceramic
spacemaintaining devices”
Start: 2011
Michael Braian
Malmö university (Supervisor: Ann
wennerberg)
“On the influence of misfit on implant
supraconstructions”
Start: 2008
Deyar Mahmood
Malmö university (Supervisors: Ann
wennerberg, per vult von Steyern)
“All ceramic bridges, choice of material used
for supporting tooth analogues, Connector
dimension and design, length of units and
different veneering techniques”
Start: 2009
Ricardo Trindade
Malmö university (Supervisors: Ann
wennerberg, Ryo Jimbo, Tomas Albrektsson)
“On Foreign Body Reactions Around Oral
Implants”
Start: 2013
Vida Krikstolaityte
vilnius university (Supervisors: Arunas
Ramanavicius at vilnius university,
Tautgirdas Ruzgas)
“Application of nanotechnology in design of
enzymatic fuel cells”
Start: 2009
Patrick Seumo
university of yaoundé I, yaounde,
Camoroon (Supervisors: Ngameni
Emmanuel at university of yaoundé I,
Nanseu Njiki Charles peguy at university of
yaoundé I, Tautgirdas Ruzgas)
“Application of nanoparticles in
electroanalysis”
Start: 2010
49
Biofilms – Research Center for Biointerfaces
Annual Report 2013
LIST OF pUBLICATIONS AT THE CENTER FROM 2013
Pihl M, Arvidsson A, Skepö M, Nilsson M, Givskov M, Tolker-Nielsen T, Svensäter G, Davies JR. Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa. Pathogens and Disease 2013;67:192-198.
Fransson H, Petersson K, Davies JR. Effects of bacterial products on the activity of odontoblast-like cells and their formation of type 1 collagen. Int Endod J 2013; doi: 10.1111/iej.12160. [Epub ahead of print].
Nylander Å, Svensäter G, Senadheera DB, Cvitkovitch DG, Davies JR, Persson K. Structural and functional analysis of the N-terminal domain of the Streptococcus gordonii adhesin Sgo0707”. PloS ONE 2013;8(5):e63768.
Dorkhan M, Svensäter G, Davies JR. Salivary pellicles on titanium and their effect on metabolic activity in Streptococcus oralis. BMC Oral Health 2013;13:32.
Wickström C, Chávez de Paz LE, Davies JR, Svensäter G. Surface-associated MUC5B mucins promote protease activity in Lactobacillus fermentum biofilms. BMC Oral Health 2013;13:43.
Pankratov, Dmitry; Zeifman, Yulia; Morozova, Olga; Shumakovich, Galina; Vasil’eva, Irina; Shleev, Sergey; Popov, Vladimir; Yaropolov, Alexander. A comparative study of biocathodes based on multiwall carbon nanotube buckypapers modified with three different multicopper oxidases. Wiley-VCH Verlag. 2013; Electroanalysis; 5 1143-1149. ISSN: 1040-0397 DOI: http://dx.doi.org/10.1002/elan.201200516
Jujic, Amra; Leosdottir, Margret; Östling, Gerd; Gudmundsson, Petri; Nilsson, Peter; Melander, Olle; Magnusson, Martin. A genetic variant of the atrial natriuretic peptide gene is associated with left ventricular hypertrophy in a non-diabetic population – the Malmo preventive project study. BioMed Central. 2013; BMC Medical Genetics; 64 1-6. ISSN: 1471-2350 DOI: http://dx.doi.org/10.1186/1471-2350-14-64
Ohlsson, Lars; Exley, Christopher; Darabi, Anna; Sandén, Emma; Siesjö, Peter; Eriksson, Håkan. Aluminium based adjuvants and their effects on mitochondria and lysosomes of phagocytosing cells. Elsevier. 2013; Journal of Inorganic Biochemistry; 128 229-236. ISSN: 1873-3344 DOI: http://dx.doi.org/10.1016/j.jinorgbio.2013.08.003
Kocherbitov, Vitaly. Application of scanning methods to distinguish between entropy and enthalpy driven phase transitions Elsevier. 2013; Current Opinion in Colloid & Interface Science; 6 510-516. ISSN: 1359-0294 DOI: http://dx.doi.org/10.1016/j.cocis.2013.11.003
Andoralov, Viktor; Falk, Magnus; Suyatin, Dmitry; Granmo, Marcus; Sotres, Javier; Ludwig, Roland; Popov, Vladimir; Schouenborg, Jens; Blum, Zoltan; Sergey, Shleev. Biofuel cell based on microscale nanostructured electrodes with inductive coupling to rat brain neurons. Nature Publishing Group. 2013; Scientific Reports; 3 art. 3270. ISSN: 2045-2322 DOI: http://dx.doi.org/10.1038/srep03270
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Falk, Magnus; Narvaez Villarrubia, Claudia; Babanova, Sofia; Atanassov, Plamen; Shleev, Sergey. Biofuel cells for biomedical applications : colonizing the animal kingdom Wiley-VCH Verlag. 2013; ChemPhysChem; 10 2045-2058. ISSN: 1439-4235 DOI: http://dx.doi.org/10.1002/cphc.201300044
Mate, Diana; Gonzalez-Perez, David; Falk, Magnus; Kittl, Roman; Pita, Marcos; De Lacey, Antonio; Ludwig, Roland; Shleev, Sergey; Alcalde, Miguel. Blood tolerant laccase by directed evolution. Elsevier. 2013; Chemistry & Biology; 2 223-231. ISSN: 1074-5521 DOI: http://dx.doi.org/10.1016/j.chembiol.2013.01.001
Alm, Kersti; El-Schich, Zahra; Falck Miniotis, Maria; Gjörloff Wingren, Anette; Janicke, Birgit; Oredsson, Stina; Mihaylova, Emilia (ed). Cells and holograms: holograms and digital holographic microscopy as a tool to study the morphology of living cells. Intech. 2013; Holography : basic principles and contemporary applications 335-351. ISBN: 978-953-51-1117-7 DOI: http://dx.doi.org/10.5772/54505
Fagerström, Anton; Kocherbitov, Vitaly; Westbye, Peter; Bergström, Karin; Mamontova, Varvara; Engblom, Johan Characterization of a plant leaf cuticle model wax, phase behaviour ofmodel wax-water systems. Elsevier. 2013; Thermochimica Acta; 42-52. ISSN: 0040-6031 DOI: http://dx.doi.org/10.1016/j.tca.2013.08.025
Fagerström, Anton; Kocherbitov, Vitaly; Westbye, Peter; Bergström, Karin; Mamontova, Varvara; Engblom, Johan; Castelani, Priscila (ed); Stock, David (ed); Puente Moran, Diana (ed). Composition of plant leaf wax, phase behavior of major components and effects of hydration. ISAA 2013 – 10th International Symposium on Adjuvants for Agrochemicals, Foz-do-Iguaco, Brazil, 22-27/4 2013. ISAA Society. 2013; Proceedings of the 10th International Symposium on Adjuvants for Agrochemicals (ISAA 2013); 257-262. ISBN: 978-90-815702-0-6
Halhalli, Mahadeo; Sellergren, Börje. Cover and uncover: chiral switching exploiting templating and layer-by-layer grafting. Royal Society of Chemistry. 2013; Chemical Communications; 64 7111-7113. ISSN: 1359-7345 DOI: http://dx.doi.org/10.1039/c3cc41989d
Graf, Gesche; Kocherbitov, Vitaly. Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique American Chemical Society ACS. 2013; The Journal of Physical Chemistry B;34 10017–10026. ISSN: 1520-6106 DOI: http://dx.doi.org/10.1021/jp404138f
Salaj-Kosla, Urszula; Poller, Sascha; Schuhmann, Wolfgang; Shleev, Sergey; Magner, Edmond. Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes. Elsevier. 2013; Bioelectrochemistry; 15-20. ISSN: 1567-5394 DOI: http://dx.doi.org/10.1016/j.bioelechem.2012.11.001
Znamenskaya, Yana. Effect of hydration on thermodynamic, rheological and structural properties of mucin. Malmö University, Faculty of Health and Society. 2013; 21. ISBN: 978-91-7104-514-0 (pdf) ISBN: 978-91-7104-513-3 (print) ISSN: 1653-5383
Fagerström, Anton; Kocherbitov, Vitaly; Ruzgas, Tautgirdas; Westbye, Peter; Bergström, Karin; Engblom, Johan. Effects of surfactants and thermodynamic activity of model active ingredient on transport over plant leaf cuticle. Elsevier. 2013; Colloids and Surfaces B: Biointerfaces; 572-579. ISSN: 0927-7765 DOI: http://dx.doi.org/10.1016/j.colsurfb.2012.11.011
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Albèr, Cathrine; Brandner, Birgit; Björklund, Sebastian; Billsten, Peter; Corkery, Robert; Engblom, Johan. Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy. Elsevier. 2013; BBA - Biomembranes; 11 2470-2478. ISSN: 0005-2736 DOI: http://dx.doi.org/10.1016/j.bbamem.2013.06.011
Ignatenko, O. V.; Sjölander, A.; Hushpulian, D. M.; Kazakov, S. V.; Ouporov, I. V.; Chubar, T. A.; Poloznikov, A. A.; Ruzgas, Tautgirdas; Tishkov, V. I.; Gorton, L.; Klyachko, N. L.; Gazaryan, I. G. Electrochemistry of chemically trapped dimeric and monomeric recombinant horseradish peroxidase. Science and Engineering Publishing Company. 2013; Advances in Biosensors and Bioelectronics; 3 25-34. ISSN: 2326-473X
Sotres, Javier; Arnebrant, Thomas. Experimental Investigations of Biological Lubrication at the Nanoscale : The Cases of Synovial Joints and the Oral Cavity. MDPI. 2013; Lubricants;4 102-131. DOI: http://dx.doi.org/10.3390/lubricants1040102
Andoralov, Viktor; Shleev, Sergey; Arnebrant, Thomas; Ruzgas, Tautgirdas. Flexible micro(bio)sensors for quantitative analysis of bioanalytes in a nanovolume of human lachrymal liquid. Springer. 2013; Anal Bioanal Chem; 11 3871-3879. ISSN: 1618-2642
Wan, Wei; Biyakal, Mustafa; Wagner, Ricarda; Sellergren, Börje; Rurack, Knut. Fluorescent sensory microparticles that “light-up” consisting of a silica core and a molecularly imprinted polymer (MIP) shell. Wiley-VCH Verlag. 2013; Angewandte Chemie International Edition; 27 7023-7027. ISSN: 1433-7851 DOI: http://dx.doi.org/10.1002/anie.201300322
Björklund, Sebastian; Engblom, Johan; Thuresson, Krister; Sparr, Emma. Glycerol and urea can be used to increase skin permeability in reduced hydration conditions. Elsevier. 2013; European Journal of Pharmaceutical Sciences; 50 638-645. ISSN: 0928-0987 DOI: http://dx.doi.org/10.1016/j.ejps.2013.04.022
Pankratov, Dmitry; Falkman, Peter; Blum, Zoltan; Shleev, Sergey. Hybrid electric power device for simultaneous generation and storage of electric energy. Royal Society of Chemistry. 2013; Energy and Environmental Science; e-pub ahead of print. DOI: http://dx.doi.org/10.1039/C3EE43413C
Kocherbitov, Vitaly; Latynis, Jekaterina; Misiunas, Audrius; Barauskas, Justas; Nuaura, Gediminas. Hydration of Lysozyme Studied by Raman Spectroscopy American Chemical Society. 2013; The Journal of Physical Chemistry B; 17 4981–4992. ISSN: 1520-6106 DOI: http://dx.doi.org/10.1021/jp4017954
Svensson, Olof; Sotres, Javier; Barrantes, Alejandro; Piñeiro, Ángel (ed); Ruso, Juan M. (ed). Investigating protein interactions at solid surfaces : in situ, non-labeling techniques. Wiley. 2013; Proteins in solution and at interfaces : methods and applications in biotechnology and materials science 113-138. ISBN: 9780470952511
Tassidis, Helena; Brokken, Leon JS; Jirström, Karin; Bjartell, Anders; Ulmert, David; Härkönen, Pirkko; Gjörloff Wingren, Anette. Low expression of SHP-2 is associated with less favorable prostate cancer outcomes. Springer. 2013; Tumor Biology; 2 637-642. ISSN: 1010-4283 DOI: http://dx.doi.org/10.1007/s13277-012-0590-1
Falk, Magnus; Andoralov, Viktor; Silow, Maria; Toscano, Miguel; Shleev, Sergey Miniature biofuel cell as a potential power source for glucose- sensing contact lenses. American Chemical Society. 2013; Analytical Chemistry; 13 6342-6348. ISSN: 0003-2700 DOI: http://dx.doi.org/10.1021/ac4006793
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Bergenzaun, Lill; Öhlin, Hans; Gudmundsson, Petri; Willenheimer, Ronnie; Chew, Michelle. Mitral annular plane systolic excursion (MAPSE) in shock: a valuable echocardiographic parameter in intensive care patients: Cardiovascular Ultrasound. BioMed Central Ltd. 2013; Cardiovascular Ultrasound; 16 1-8. ISSN: 1476-7120 DOI: http://dx.doi.org/10.1186/1476-7120-11-16
Wadsäter, Maria; Barauskas, Justas; Nylander, Tommy; Tiberg, Fredrik Nonlamellar lipid liquid crystalline model surfaces for biofunctional studies. Royal Society of Chemistry. 2013; Soft Matter; 37 8815-8819. ISSN: 1744-683X DOI: http://dx.doi.org/10.1039/c3sm51385h
Shao, Minling; Zafar, Muhammad Nadeem; Falk, Magnus; Ludwig, Roland; Sygmund, Christoph; Peterbauer, Clemens; Guschin, Dmitrii; MacAodha, Domhnall; O’Conghaile, Peter; Leech, Donal; Toscano, Miguel; Shleev, Sergey; Schuhmann, Wolfgang; Gorton, Lo. Optimization of a membraneless glucose/oxygen enzymatic fuel cell based on a bioanode with high coulombic efficiency and current density. Wiley-VCH Verlag. 2013; ChemPhysChem; 10 2260-2269. ISSN: 1439-4235 DOI: http://dx.doi.org/10.1002/cphc.201300046
Pita, Marcos; Gutierrez-Sanchez, Cristina; Toscano, Miguel; Shleev, Sergey; De Lacey, Antonio. Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode. Elsevier. 2013; Bioelectrochemistry; 94 69-74. ISSN: 1567-5394 DOI: http://dx.doi.org/10.1016/j.bioelechem.2013.07.001
Seghatoleslami, Sepideh; Ohlsson, Lars; Kristina, Hamberg; Carlsson, Peter; Ericson, Dan; Ljunggren, Lennart Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction. Scientific Research Publishing. 2013; American Journal of Molecular Biology; 3 148-152. ISSN: 2161-6620 DOI: http://dx.doi.org/[10.4236/ajmb.2013.33019
Ericson, Dan; Hamberg, Kristina; Bratthall, Gunilla; Sinkiewicz-Enggren, Gabriela; Ljunggren, Lennart. Salivary IgA response to probiotic bacteria and mutans streptococci after the use of chewing gum containing Lactobacillus reuteri. Wiley Blackwell. 2013; Pathogens and Disease; 3 82-87. ISSN: 2049-632X DOI: http://dx.doi.org/10.1111/2049-632X.12048
Panrkatov, Dmitry; Blum, Zoltan; Suyatin, Dmitry; Popov, Vladimir; Shleev, Sergey. Self-charging electrochemical biocapacitor. John Wiley & Sons. 2014; ChemElectroChem; 2, 343-346. ISSN: 2196-0216 DOI: http://dx.doi.org/10.1002/celc.201300142
Björklund, Sebastian; Ruzgas, Tautgirdas; Nowacka, Agnieszka; Dahi, Ihab; Topgaard, Daniel; Sparr, Emma; Engblom, Johan. Skin membrane electrical impedance properties under the influence of a varying water gradient. Elsevier. 2013; Biophysical Journal; 12 2639-2650. ISSN: 0006-3495 DOI: http://dx.doi.org/10.1016/j.bpj.2013.05.008
Mate, Diana; Garcia-Ruiz, Eva; Shubin, Vladimir; Falk, Magnus; Shleev, Sergey; Ballesteros, Antonio; Alcalde, Miguel. Switching from blue to yellow: altering the spectral properties of a high redox potential laccase by directed evolution. Informa Healthcare. 2013; Biocatalysis and Biotransformation; 1 8-21. ISSN: 1024-2422 DOI: http://dx.doi.org/10.3109/10242422.2012.749463
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Wagner, Ricarda; Wan, Wei; Biyakal, Mustafa; Benito Pena, Elena; Moreno-Bondi, Maria; Lazraq, Issam; Sellergren, Börje; Rurack, Knut. Synthesis, spectroscopic, and analyte-responsive behavior of a polymerizable naphthalimide-based arboxylate probe and molecularly imprinted polymers prepared thereof. American Chemical Society. 2013; Journal of Organic Chemistry; 4 1377-1389. ISSN: 0022-3263 DOI: http://dx.doi.org/10.1021/jo3019522
Olsson, Gustaf; Karlsson, Björn; Schillinger, Eric; Sellergren, Börje; Nicholls, Ian. Theoretical studies of 17-beta-estradiol-imprinted prepolymerization mixtures : insights concerning the roles of cross-linking and functional monomers in template complexation and polymerization. American Chemical Society. 2013; Industrial and Engineering Chemistry Research; 39 13965-13970. ISSN: 0888-5885 DOI: http://dx.doi.org/10.1021/ie401115f
Znamenskaya, Yana; Sotres, Javier; Gavryushov, Sergei; Engblom, Johan; Arnebrant, Thomas; Kocherbitov, Vitaly. Water Sorption and Glass Transition of Pig Gastric Mucin Studied by QCM-D. American Chemical Society ACS. 2013; Journal of Physical Chemistry B; 8 2554–2563. ISSN: 1520-6106 DOI: http://dx.doi.org/10.1021/jp311968b
Sotres, J., Barrantes, A., Lindh, L., Arnebrant, T. Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces. Caries Research, 48: 98-110, 2013.
Barrantes, A., Arnebrant, T., Lindh, L. Characteristics of saliva films adsorbed onto dental materials studied by QCM-D. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013 DOI: http://dx.doi.org/10.1016/j.colsurfa.2013.05.054
Cecchinato F, Xue Y, Karlsson J, He W, Wennerberg A, Mustafa K, Andersson M, Jimbo R. In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating. J Biomed Mater Res A. 2013 Dec 12. doi: 10.1002/jbm.a.35062. [Epub ahead of print]
Ostman PO, Wennerberg A, Ekestubbe A, Albrektsson T. Immediate Occlusal Loading of NanoTite™ Tapered Implants: A Prospective 1-Year Clinical and Radiographic Study. Clin Implant Dent Relat Res. 2013 Dec;15 (6):809-18. doi: 10.1111/j.1708-8208.2011.00437.x. Epub 2012 Jan 17.
Jimbo R, Anchieta R, Baldassarri M, Granato R, Marin C, Teixeira HS, Tovar N,Vandeweghe S, Janal MN, Coelho PG Histomorphometry and bone mechanical property evolution around different implant systems at early healing stages: an experimental study in dogs. Implant Dent. 2013 Dec; 22(6): 596-603. doi: 10.1097/ID.0b013e31829f1f4b.
Naito Y, Terukina T, Galli S, Kozai Y, Vandeweghe S, Tagami T, Ozeki T, Ichikawa T, Coelho PG, Jimbo R. The effect of simvastatin-loaded polymeric microspheres in a critical size bone defect in the rabbit calvaria. Int J Pharm. 2013 Dec 1;461(1-2):157-162. doi: 10.1016/j.ijpharm.2013.11.046. [Epub ahead of print]
Jimbo R, Giro G, Marin C, Granato R, Suzuki M, Tovar N, Lilin T, Janal M, Coelho PG. Simplified drilling technique does not decrease dental implant osseointegration: a preliminary report. J Periodontol. 2013 Nov; 84(11): 1599-605. doi: 10.1902/jop.2012.120565. Epub 2012 Dec 5.
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Yeniyol S, Jimbo R, Marin C, Tovar N, Janal MN, Coelho PG The effect of drilling speed on early bone healing to oral implants. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Nov; 116(5): 550-5. doi: 10.1016/j.oooo.2013.07.001. Epub 2013 Sep 20.
Altmann B, Kohal RJ, Steinberg T, Tomakidi P, Bächle-Haas M, Wennerberg A, Att W. Distinct cell functions of osteoblasts on UV-functionalized titanium- and zirconia-based implant materials are modulated by surface topography. Tissue Eng Part C Methods. 2013 Nov; 19(11): 850-63. doi: 10.1089/ten.TEC.2012.0695. Epub 2013 Jun 4.
Galli S, Jimbo R, Andersson M, Bryington M, Albrektsson T Surface characterization and clinical review of two commercially available implants. Implant Dent. 2013 Oct; 22(5): 507-18. doi: 10.1097/ID.0b013e318294308f.
Vandeweghe S, Coelho PG, Vanhove C, Wennerberg A, Jimbo R. Utilizing micro-computed tomography to evaluate bone structure surrounding dental implants: a comparison with histomorphometry J Biomed Mater Res B Appl Biomater. 2013 Oct; 101(7): 1259-66. doi: 10.1002/jbm.b.32938. Epub 2013 May 10.
Coelho PG, Teixeira HS, Marin C, Witek L, Tovar N, Janal MN, Jimbo R. The in vivo effect of P-15 coating on early osseointegration. J Biomed Mater Res B Appl Biomater. 2013 Sep 16. doi: 10.1002/jbm.b.33020. [Epub ahead of print]
Albrektsson T, Dahlin C, Jemt T, Sennerby L, Turri A, Wennerberg A . Is Marginal Bone Loss around Oral Implants the Result of a Provoked Foreign Body Reaction? Clin Implant Dent Relat Res. 2013 Sep 4. doi: 10.1111/cid.12142. [Epub ahead ofprint]
Vandeweghe S, Nicolopoulos C, Thevissen E, Jimbo R, Wennerberg A, De Bruyn H. Immediate loading of screw-retained all-ceramic crowns in immediate versus delayed single implant placement. Int J Prosthodont. 2013 Sep-Oct; 26(5):458-64. doi: 10.11607/ijp.3075.
Chowdhary R, Halldin A, Jimbo R, Wennerberg A. Influence of Micro Threads Alteration on Osseointegration and Primary Stability of Implants: An FEA and In Vivo Analysis in Rabbits. Clin Implant Dent Relat Res. 2013 Aug 27. doi: 10.1111/cid.12143. [Epub ahead of print]
Tovar N, Jimbo R, Gangolli R, Perez L, Manne L, Yoo D, Lorenzoni F, Witek L,Coelho PG. Evaluation of bone response to various anorganic bovine bone xenografts: an experimental calvaria defect study Int J Oral Maxillofac Surg. 2013 Aug 12. pii: S0901-5027(13)00298-1. doi: 10.1016/j.ijom.2013.07.005. [Epub ahead of print]
De Wilde EA, Jimbo R, Wennerberg A, Naito Y, Coucke P, Bryington MS, Vandeweghe S, De Bruyn H. The Soft Tissue Immunologic Response to Hydroxyapatite-Coated Transmucosal Implant Surfaces: A Study in Humans. Clin Implant Dent Relat Res. 2013 Aug 5. doi: 10.1111/cid.12128. [Epub ahead of print]
Hirakawa Y, Jimbo R, Shibata Y, Watanabe I, Wennerberg A, Sawase T. Accelerated bone formation on photo-induced hydrophilic titanium implants: anexperimental study in the dog mandible. Clin Oral Implants Res. 2013 Aug; 24 Suppl A100:139-44. doi: 10.1111/j.1600-0501.2011.02401.x. Epub 2012 Jan 17.
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Vandeweghe S, Leconte C, Ono D, Coelho PG, Jimbo R. Comparison of histological and three-dimensional characteristics of porous titanium granules and deproteinized bovine particulate grafts used for sinus floor augmentation in humans: a pilot study. Implant Dent. 2013 Aug; 22(4):339-43. doi: 10.1097/ID.0b013e3182938d03.
Yoo D, Tovar N, Jimbo R, Marin C, Anchieta RB, Machado LS, Montclare J, Guastaldi FP, Janal MN, Coelho PG Increased osseointegration effect of bone morphogenetic protein 2 on dental implants: An in vivo study J Biomed Mater Res A. 2013 Jul 12. doi: 10.1002/jbm.a.34862.[Epub ahead of print]
Chrcanovic BR, Martins MD, Wennerberg A. Immediate Placement of Implants into Infected Sites: A Systematic Review. Clin Implant Dent Relat Res. 2013 Jul 2. doi: 10.1111/cid.12098. [Epub ahead of print]
Jimbo R, Halldin A, Janda M, Wennerberg A, Vandeweghe S. Vertical fracture and marginal bone loss of internal-connection implants: a finite element analysis. Int J Oral Maxillofac Implants. 2013 Jul-Aug; 28(4):e171-6. doi:10.11607/jomi.3052.
Wennerberg A, Jimbo R, Stübinger S, Obrecht M, Dard M, Berner S. Nanostructures and hydrophilicity influence osseointegration: a biomechanical study in the rabbit tibia. Clin Oral Implants Res. 2013 Jun 19. doi: 10.1111/clr.12213. [Epub ahead of print]
Chowdhary R, Jimbo R, Thomsen CS, Carlsson L, Wennerberg A. The osseointegration stimulatory effect of macrogeometry-modified implants: a study in the rabbit. Clin Oral Implants Res. 2013 Jun 18. doi: 10.1111/clr.12212. [Epub ahead of print]
Jimbo R, Tovar N, Yoo DY, Janal MN, Anchieta RB, Coelho PG. The effect of different surgical drilling procedures on full laser-etched microgrooves surface-treated implants: an experimental study in sheep. Clin Oral Implants Res. 2013 Jun 17. doi: 10.1111/clr.12216. [Epub ahead ofprint]
Moura CC, Machado JR, Silva MV, Rodrigues DB, Zanetta-Barbosa D, Jimbo R, TovarN, Coelho PG. Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces.Biomed Mater. 2013 Jun; 8(3): 035010. doi: 10.1088/1748-6041/8/3/035010. Epub 2013 Apr 19.
He W, Andersson M, de Souza PP, de Souza Costa CA, Muñoz EM, Schwartz-Filho HO, Hayashi M, Hemdal A, Fredel A, Wennerberg A, Jimbo R. Osteogenesis-inducing calcium phosphate nanoparticle precursors applied to titanium surfaces. Biomed Mater. 2013 Jun; 8(3):035007. doi: 10.1088/1748-6041/8/3/035007. Epub 2013 Apr 5.
Moura CG, Souza MA, Kohal RJ, Dechichi P, Zanetta-Barbosa D, Jimbo R, Teixeira CC, Teixeira HS, Tovar N, Coelho PG. Evaluation of osteogenic cell culture and osteogenic/peripheral blood mononuclear human cell co-culture on modified titanium surfaces. Biomed Mater. 2013 Jun; 8(3):035002. doi: 10.1088/1748-6041/8/3/035002. Epub 2013 Mar 26.
Bryington MS, Hayashi M, Kozai Y, Vandeweghe S, Andersson M, Wennerberg A, Jimbo R The influence of nano hydroxyapatite coating on osseointegration after extendedhealing periods. Dent Mater. 2013 May; 29(5): 514-20. doi: 10.1016/j.dental.2013.02.004. Epub 2013 Mar 13.
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Bougas K, Jimbo R, Vandeweghe S, Hayashi M, Bryington M, Kozai Y, Schwartz-Filho HO, Tovar N, Adolfsson E, Ono D, Coelho PG, Wennerberg A. Bone apposition to laminin-1 coated implants: histologic and 3D evaluation. Int J Oral Maxillofac Surg. 2013 May; 42(5):677-82. doi: 10.1016/j.ijom.2012.11.008. Epub 2012 Dec 8.
Takeshita K, Funaki K, Jimbo R, Takahashi T.Nasopalatine duct cyst developed in association with dental implant treatment: A case report and histopathological observation. J Oral Maxillofac Pathol. 2013 May; 17(2): 319. doi: 10.4103/0973-029X.119761.
Wennerberg A, Bougas K, Jimbo R, Albrektsson T. Implant coatings: new modalities for increased osseointegration. Am J Dent. 2013 Apr; 26 (2): 105-12.
Meirelles L, Uzumaki ET, Lima JH, Muller CA, Albrektsson T, Wennerberg A, Lambert CS. A novel technique for tailored surface modification of dental implants – a step wise approach based on plasma immersion ion implantation. Clin Oral Implants Res. 2013 Apr; 24 (4): 461-7. doi: 10.1111/j.1600-0501.2011.02352.x. Epub 2011 Dec 15.
Anchieta RB, Baldassarri M, Guastaldi F, Tovar N, Janal MN, Gottlow J, Dard M, Jimbo R, Coelho PG. Mechanical Property Assessment of Bone Healing around a Titanium-Zirconium Alloy Dental Implant. Clin Implant Dent Relat Res. 2013 Mar 25. doi: 10.1111/cid.12061. [Epub ahead of print]
Chowdhary R, Jimbo R, Thomsen C, Carlsson L, Wennerberg A.Biomechanical evaluation of macro and micro designed screw-type implants: an insertion torque and removal torque study in rabbits Clin Oral Implants Res. 2013 Mar; 24 (3): 342-6. doi: 10.1111/j.1600-
Rosa MB, Albrektsson T, Francischone CE, Filho HO, Wennerberg A. Micrometric characterization of the implant surfaces from the five largest companies in Brazil, the second largest worldwide implant market. Int J Oral Maxillofac Implants. 2013 Mar-Apr; 28 (2): 358-65. doi: 10.11607/jomi.2791.
Hayashi M, Jimbo R, Xue Y, Mustafa K, Andersson M, Wennerberg A. Photocatalytically induced hydrophilicity influences bone remodelling at longer healing periods: a rabbit study. Clin Oral Implants Res. 2013 Feb 26. doi: 10.1111/clr.12138. [Epub ahead of print]
Odatsu T, Jimbo R, Wennerberg A, Watanabe I, Sawase T Effect of polishing and finishing procedures on the surface integrity of restorative ceramics. Am J Dent. 2013 Feb; 26 (1): 51-5.
Wennerberg A, Svanborg LM, Berner S, Andersson M. Spontaneously formed nanostructures on titanium surfaces. Clin Oral Implants Res. 2013 Feb; 24 (2): 203-9. doi: 10.1111/j.1600-0501.2012.02429.x. Epub 2012 Feb 15.
Valverde GB, Jimbo R, Teixeira HS, Bonfante EA, Janal MN, Coelho PG. Evaluation of surface roughness as a function of multiple blasting processing variables. Clin Oral Implants Res. 2013 Feb; 24 (2): 238-42. doi: 10.1111/j.1600-0501.2011.02392.x. Epub 2011 Dec 20.
Chowdhary R, Halldin A, Jimbo R, Wennerberg A.Evaluation of stress pattern generated through various thread designs of dental implants loaded in a condition of immediately after placement and on osseointegration--an FEA study. Implant Dent. 2013 Feb; 22 (1): 91-6. doi: 10.1097/ID.0b013e31827daf55.
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Barkarmo S, Wennerberg A, Hoffman M, Kjellin P, Breding K, Handa P, Stenport V. Nano-hydroxyapatite-coated PEEK implants: a pilot study in rabbit bone. J Biomed Mater Res A. 2013 Feb; 101(2): 465-71. doi: 10.1002/jbm.a.34358. Epub 2012 Aug 3.
Bougas K, Jimbo R, Vandeweghe S, Tovar N, Baldassarri M, Alenezi A, Janal M, Coelho PG, Wennerberg In Vivo Evaluation of a Novel Implant Coating Agent: Laminin-1. A Clin Implant Dent Relat Res. 2013 Jan 11. doi: 10.1111/cid.12037. [Epub ahead of print]
Browaeys H, Vandeweghe S, Johansson CB, Jimbo R, Deschepper E, De Bruyn H. The histological evaluation of osseointegration of surface enhanced microimplants immediately loaded in conjunction with sinuslifting in humans. Clin Oral Implants Res. 2013 Jan; 24 (1): 36-44. doi:10.1111/j.1600-0501.2011.02398.x. Epub 2012 Jan 6.
Giro G, Tovar N, Marin C, Bonfante EA, Jimbo R, Suzuki M, Janal MN, Coelho PG.The effect of simplifying dental implant drilling sequence on osseointegration: an experimental study in dogs. Int J Biomater. 2013;2013:230310. doi: 10.1155/2013/230310. Epub 2013 Jan 30.
Guastaldi FP, Yoo D, Marin C, Jimbo R, Tovar N, Zanetta-Barbosa D, Coelho PG. Plasma treatment maintains surface energy of the implant surface and enhances osseointegration. Int J Biomater. 2013;2013:354125. doi: 10.1155/2013/354125. Epub 2013 Jan 10.
Marin C, Jimbo R, Lorenzoni FC, Witek L, Teixeira H, Bonfante E, Gil J, Granato R, Tovar N, Coelho PG. Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible. Int J Dent. 2013;2013:296391. doi: 10.1155/2013/296391. Epub 2013 Oct 27.
Alenezi A, Naito Y, Andersson M, Chrcanovic BR, Wennerberg A, Jimbo R. Characteristics of 2 Different Commercially Available Implants with or without Nanotopography. Int J Dent. 2013;2013:769768. doi: 10.1155/2013/769768. Epub 2013 Oct 2.
Bonfante EA, Granato R, Marin C, Jimbo R, Giro G, Suzuki M, Coelho PG.Biomechanical testing of microblasted, acid-etched/microblasted, anodized, and discrete crystalline deposition surfaces: an experimental study in beagle dogs.Int J Oral Maxillofac Implants. 2013 Jan-Feb; 28(1):136-42. doi: 10.11607/jomi.2331.
Malekzadeh B, Tengvall P, Ohrnell LO, Wennerberg A, Westerlund A. Effects of locally administered insulin on bone formation in non-diabetic rats. J Biomed Mater Res A. 2013 Jan; 101(1):132-7. doi: 10.1002/jbm.a.34313. Epub 2012 Jul 24.
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CONTRIBUTIONS TO AND pARTICIpATION IN CONFERENCES AND wORkSHOpS
Ann Wennerberg. “Biomechanical, histomorphometrical and gene expression analyses used to evaluate the osseointegration process” IADR, Seattle, USA March 20-23 2013. Invited lecture
Ann Wennerberg. “Designing for life: Today and in the future” Nobel Biocare Global Symposium 2013. June 20–22 New York USA. Invited lecture
Ann Wennerberg. “Dental Implant surfaces: possibilities and threats” IN 2013 Latin American Osseointegration Congress. Sap Paulo Brazil September 25th to 28th 2013. Invited lecture
Ann Wennerberg. “Why and when do implant fail. Future challenges in prosthodontics rehabilitation of oral function” CORE October 23–25, Beijing, China 2013. Invited lecture
Ann Wennerberg. “Reasons for failure of oral implants” George Zarb clinical research lecture. Deans lecture series. Toronto University, Toronto, Canada November 25, 2013. Invited lecture
Ann Wennerberg. “On macro-, micro-, and nanoaspects of implant surfaces and their importance for osseointegration” 5th International Conference on Mechanics of Biomaterials and Tissues. Sitges, Spain 8–12 December 2013. Invited lecture
Bertil Kinnby. “PAI-2 inhibits bacterial proteases” XIV International Workshop on Molecular and Cellular Biology of Plasminogen Activation, Notre Dame, IN, USA. Håkan Eriksson. “Aluminium adjuvant-induced mitochondrial alterations, Tenth Keele Meeting on Aluminium”, Winchester, UK.
Börje Sellergren. “Molecularly imprinted polymers for environmental technologies”. 11th international conference on Materials Chemistry. Warwick, UK, July 2013. Keynote speaker
Börje Sellergren. “Molecularly imprinted polymers in biomarker analysis” Biomarker workshop, HS, October 2013.
Böje Sellergren. “Designing MIPs for food safety applications: endocrine disruptors, antibi-otics, dioxins “. Sino German conference on Food Safety, Beijng, September, 2013.
Gunnel Svensäter. “Infection control on the biofilm level” University of Minneapolis, MN, USA. Invited speaker
Johan Engblom. “A Water Gradient can be used to Regulate Biophysical Properties of Skin and Mucosa”. The Biennial Australian Colloidal and Interface Symposium, Australia 3–7/2 2013.
oRAl pResentAtions 2013
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Johan Engblom. “A Water Gradient can be used to Regulate Biophysical Properties of Skin and Mucosa”. Applied Mathematics , ANU, Canberra Australia 18/2 2013
Albèr C. Prospects in controlled uptake, delivery and release Symposium SP, Stockholm 23/10 2013.
Liselott Lindh, Alejandro Barrantes, Thomas Arnebrant. “Characteristics of saliva films adsorbed onto different dental materials” IADR, Seattle
Sergey Shleev. Invited Lecture entitled: “On the possibility of uphill intramolecular electron transfer in multicopper oxidases” at the mini-symposium on Bioelectrochemical systems (BES), February 01, 2013, Lund, Sweden.
Sergey Shleev. Invited Lecture entitled: “Recent advances in biofuel cells and biobatteries” at the 10th International Electrochemistry Days, September 05, 2013, Konya, Turkey.
Sergey Shleev. Invited Lecture entitled: “Teardrop analysis” at the World Diabetes Day, November 09, 2013, Malmö, Sweden.
Sergey Shleev. Tutorial Lecture entitled: “Recent advances in biofuel cells” at the 6th International Workshop SMCBS ”Surface Modification for Chemical and Biochemical Sensing”, November 11, 2013, Lochow, Poland
Vitaly Kocherbitov. “Application of Humidity-Scanning QCM-D Method for Surfactant Phase Studies” ECIS2013, Sofia, Bulgaria.
Vitaly Kocherbitov. “Hydration-induced phase and glass transitions in soft matter films revealed by QCM-D” COST workshop The balance of interactions in soft-matter systems, Nicosia, Cyprus. Invited speaker
contAct infoRmAtionContact Information
Biofilms – Research Center for Biointerfaces
Malmö University
Faculty of Health and Society
205 06 Malmö
Sweden
Center director: Doctor Anna Holmberg
Tel: +46 40 665 79 35
e-mail: [email protected]
www.mah.se/biofilms