biofilms research center for biointerfaces · from the center activities, now established as two of...

BIO FILMS

INTERFACES Biofilms – Research Center for Biointerfaces

Biofilms –Research Center for Biointerfaces A translational research programme at Malmö University

Funded by the Knowledge foundation

Progress report #6

January 1st, 2010 – December 31

st, 2010

Cover illustration

Biofilms develop spontaneously on a surface in contact with a liquid phase containing

biomolecules and micro-organisms. The initial phase in the development is rapid

adsorption of surface active molecules, notably macromolecules such as proteins, to the

surface forming an initial ―conditioning film‖. Next step is attachment of

microorganisms. These organisms grow and interact with molecules from the liquid

phase, cell produced matrix, and other organisms in the formation of a biological film

(biofilm). Organisms and molecules within the biofilm possess unique characteristics not

observed for the same species suspended or dissolved in the associated liquid phase.

Contact information

Biofilms – Research Center for Biointerfaces

Faculty of Health and Society

Malmö University

SE-205 06 MALMÖ, Sweden

Center director: Assoc. Prof. Johan Engblom (JE)

Tel: +46-(0)706-08 75 25 (JE); +46-(0)40-66 57 486 (Adm. Coordinator Eva Nilsson)

e-mail: [email protected]

www.mah.se/biofilms

Visiting adress:

Skåne University Hospital, SUS (Entrance 49)

MALMÖ

Biofilms –Research Center for Biointerfaces Progress Report 2010

Mah: Malmö University; LU: Lund University; KTH: The Royal Institute of Technology; LuTH: Luleå Technical University; UU: Uppsala

University; LiU: Linköping University; KI: Karolinska Institute; OD: Faculty of Odontology; HS: Faculty of Health and Society; TS: School of

Technology. KKs: The Knowledge Foundation; VR: The Swedish Research Council; EU: European Union; FP6: 6th Framework programme;

FP7: 7th Framework programme; BMT: Biomedical Technology BMMT: Biomedical Methods and Technology; MS: Materials Science; FLÄK:

The Research School in Pharmaceutical Science; BMA: Biomedical Laboratory Science; TELMah: Technology, Economy and Leadership at Mah

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1 List of Center Members During the period of report the Center has comprised the following members (permanent

staff, postdocs, PhD-students and technical and administrative staff).

1.1 Permanent staff

Johan Engblom, Assoc Prof., Director 2008-

Thomas Arnebrant, Prof., Director 2005-2007, Vice Director 2010

Tautgirdas Ruzgas, Prof.

Gunilla Nordin-Fredrikson, Prof. (also part time LU)

Per Ståhle von Schwerin, Prof.

Ann Wennerberg, Prof.

Gunnel Svensäter, Prof.

Ali Massih, Prof.

Håkan Ericsson, Assoc. Prof.

Vitaly Kocherbitov, Assoc. Prof.

Liselott Lindh, Assoc. Prof.

Anette Gjörloff-Wingren, Assoc. Prof.

Zoltan Blum, Assoc. Prof.

Liu-Ying Wei, Assoc. Prof.

Julia Davies, Assoc. Prof.

Bertil Kinnby, Assoc. Prof.

Christina Bjerkén, Assoc. Prof.

Claes Wickström, Assoc. Prof.

Lars Ohlsson, Dr

Tove Sandberg, Dr.

Maria Stollenwerk, Dr.

Sergey Shleev, Dr.

Anna Ketelsen, Dr.

1.2 Junior researchers and postdocs

Olof Svensson, Dr.

Olga Santos, Dr.

Laura Varas, Dr.

Jessica Neilands, Dr.

Luis Chavez de Paz, Dr.

Javier Sotres, Dr

Ida Svendsen, Dr

Peter Nilsson, Dr

Alejandro Barrantes, Dr

Jovice Bon Singh Ng, Dr

Ryo Jimbo, Dr

Viktor Andoralov, Dr

1.3 PhD students

Jildiz Hamit Eminovski

Ulf Hejman

Alma Masic







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Maria Pihl

Sebastian Björklund (enrolled at LU)

Anton Fagerström

Yana Znamenskaya

Adnan Safdar

Christian Alfredsson Kindblom

Marjan Dorkhan

Kostas Bougas

Magnus Falk

Peter Lamberg

Cathrine Albér

Mariko Hayashi

Lory Melin Svanborg

Tuerdi Maimaitiyili

1.4 Technical and administrative staff

Eva Nilsson, Administrative coordinator

Ulrika Troedsson, Technician

Agnethe Henriksson, Technician

Madeleine Blomqvist, Technician

Lina Pedersen, Technician

1.5 MSc students

BMMT Master at HS 2010-2012

Joynul Abedin

Ameena Daftani

Endale Asmare Hailu

Rakibul Islam

Lutfor Islam

Abu Sayeed Khan

Jabed Khandaker

Shadi Movahed Bashiri

Shifa Saleem

Selva Kumar Subramanian

Mohammed B Sunmonu

Inger Anne Tveit

Surendra Vutti

Shaheen Mohammad Syful

Islam


Haddel Ali Shoker

Joy Chia

Ihab Dahi

Payam Delfani

Susanna Tarasco

Mohammad Zahir Uddin

Petra Wicktor


Aseel Albayati

Sheima Sultan Kadir

Eleonora Dahlquist

Rula Bahran

Peter Lamberg

Marianne Mårtensson

Patrik Bauer

MS Master at HS & TS 2008-2010

Maihemutijiang Maimaiti

Carl Mikaelsson

Ajigul Nuermaimaiti

Oyetunji Oladele Kazeem

Wureguli Reheman

Christian Ukoha Oji

Erik Öberg

Simayijiang Zhayida

In addtition to these two year Master programmes, the Faculty of Odontology (OD) offers

the Dentistry programme which is a continuous five year program to Master level.







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1.6 Management and boards

Biofilms – Research Center for Biointerfaces is managed on a daily basis by the Center

director and an Executive group, constituted by the heads of the individual research

groups. The Steering group constitutes a link to central Malmö University, and involves

the Deputy Vice-Chancellor and the Deans of the three faculties/schools involved in the

research activities of the Center. The Reference group is an advisory board to the Director

and contains representatives from Industry, Medicon Valley Alliance, Malmö University

and other universities.

Executive group

Johan Engblom, Assoc. Prof., Director & Chairman for 2008-

Thomas Arnebrant, Prof., Director & Chairman for 2005-2007

Per Ståhle von Schwerin, Prof.

Gunnel Svensäter, Prof.

Ann Wennerberg, Prof.

Gunilla Nordin-Fredrikson, Prof.

Håkan Eriksson, Assoc. Prof.

Steering group

Eva Engquist, Deputy Vice-Chancellor, Malmö University, Chairman

Margareta Östman, Prof., Dean Faculty of Health and Society

Naser Eftekharian, Head School of Technology Malmö University

Lars Bondemark, Prof., Dean Faculty of Odontology

Reference group 2009-2011

Martin Malmsten, Prof., Uppsala University, Chairman

Ian Hamilton, Prof. em., University of Manitoba, Canada

Peter Nordström, Senior Project Manager, Medicon Valley Alliance

Yngve Sommarin, Dr., R&D Manager Euro-Diagnostica/Wieslab AB

Magnus Christensson, Dr., R&D Manager AnoxKaldnes AB

Markus Johnsson, Dr., Senior Director Pharm. Development Camurus AB

Eva Engquist, Deputy Vice-Chancellor, Malmö University

Zoltan Blum, Assoc. Prof., Malmö University







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2 The Director´s Report Biofilms - Research Center for Biointerfaces that has been established as a vital asset for

Malmö University, is well reputed and carries a strong trademark within the university.

Also, an increased regional awareness of the center’s activities, including other academic

institutions, industry and public sector, can be noted. Industry partners comment that the

center has positioned Malmö University as an attractive partner and that it is an enterprise

that benefits both industry and academia.

During the period 2005-2010 the center has expanded to twice its original size, now

comprising 38 projects and 32 industry partners. Two strong research areas have evolved

from the center activities, now established as two of the current eight research profiles at

the university, i.e. ―Oral health‖ and ―Biointerfaces‖. External research funding for the

entire university amounts to 107 MSEK in 2010, where Biofilms –Research Center for

Biointerfaces contributes with 20%. Regarding output in terms of international journal

publications the center share is 23% of the total university production 2010.

Collaboration with external parties is a corner stone in the university strategy and the

center is often put forward as a good example.

Viktoria Fröjd, Helena Tassidis (enrolled at LU) and Rickard Hägglund (enrolled at LTH)

successfully defended their PhD-theses in 2010, as did Ulf Hejman and Adnan Safdar

defending their licentiate theses. Claes Wickström and Christina Bjerkén were accepted

as Associate Professors and Sergey Shleev has pending applications as Associate

Professor (Malmö) and Habil. Doctor of Science (Russia). Drs Olga Santos and Olof

Svensson left their positions as researchers during the year, while five new post docs and

five PhD students (three at Malmö University and two enrolled at Lund University) were

recruited. Two PhD-students had research stays at partner sites, Alma Masic at University

of Guelph University, Canada, and Anton Fagerström at AkzoNobel Surface Chemistry

AB, Stenungsund, Sweden. Several senior researchers also payed visits to universities

outside Sweden (e.g. China, Russia, Lithuania, Germany and UK).

During 2010 we have produced 59(67) publications in international journals, 5 book

chapters and 3 proceeding papers. Publications are in journals such as Trends in

Immunology (impact factor 8.8), Atherosclerosis, Trombosis and Vascular Biology (7.5)

Journal of Controlled Release (6.0), Biofouling (4.4), Langmuir (3.9), Journal of

Endodontics (3.0), Clinical Oral Implant Research (2.9), International Journal of Solids

and Structure (1.8) and Mathematical Biosciences (1.3). High impact factors indicate

substantial scientific quality of the research carried out within the Center. In addition to

this, approximately 51 oral and 21 poster presentations were made at national and

international meetings. In reviewing the number citations over the past five years on

papers produced by the Center members we sum up 7763 for permanent staff and 587 for

junior researchers and post-docs. Five members have more than 500 citations each, Profs.

Tautgirdas Ruzgas, Gunilla Nordin Fredrikson, Thomas Arnebrant, Ann Wennerberg and

Dr Sergey Schleev. Center members have also refereed papers for international journals

on a regular basis.







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Members of the Center take part in The National Research School of Odontology (Ann

Wennerberg (coordinator), Gunnel Svensäter (local coordinator) and Julia Davies,

supervisors), and The Research School in Pharmaceutical Sciences, LU (Johan Engblom,

supervisor). Most researchers participate in undergraduate (the BMA and TELMah

programmes) and/or graduate teaching (the BMMT, MS and Dentistry programmes), as

well as PhD-student supervision. We strive to further integrate education (BSc, MSc,

PhD), a cornerstone for the future of the Center. Particularly, one goal is to further

integrate Master-level education into our research activities also at an operational level in

specific projects.

Research collaboration with industry is active in all four focus areas of the Center and

during 2010 we attracted Novosense AB, Biogaia AB, ACO Hud Nordic AB, YKI AB

and Eviderm Institute AB as partners in new research projects. Although border lines

between the focus areas are not always clear cut, the company involvement distribute

according to i) Eucaryotic cell-surface interactions (Promimic, PHI), ii) Molecular

transport phenomena (AkzoNobel, YKI, ACO Hud Nordic, Eviderm Institute), iii)

Molecular interactions at biointerfaces (Camurus, EuroDiagnostica, Promimic,

Novosense, Anordica, Arcam, Bioglan, Galenica) and iv) Microbial biofilms (ArlaFoods,

AnoxKaldnes, Gambro, Arcam, Biogaia). We also have close collaboration with Medeon

AB and Medicon Valley Alliance.

Center members are partners in the collaborative EU FP7 funded project ―Three-

dimensional nanobiostructure-based self-contained devices for biomedical application‖

(Dr Sergey Shleev, coordinator) and continued to be involved in the EU FP6 Marie Curie

Research Training Networks (MCRTNs), ―Bio-interfaces: from molecular understanding

to applications‖. We are also involved in three EU Interreg programmes; ―Valorisation of

knowledge intensive ideas in the South Baltic area‖ (SB-VALOR), ―Öresund forum for

innovation within nano-, bio- and medical technology‖ (FinNBMT) and ―Öresund

materials innovation comunity‖ (Ö-MIC).

Center members have been responsible for arranging three workshops, ―Biomaterials –

from fundamentals to Market Application‖ (Biofilms 6th

Annual workshop), ―Biofilms

Members Day‖ and together with BIOSUM, Gothenburg; ―Biomaterials in medicine‖,

(within the National Research School of Odontology), one salivary symposium at IADR

in Barcelona and a kick –off meeting for a new research project funded by KKs. The

workshop ―Choice of and collaboration with CRO´s in pharmaceutical development‖

(launched by the Swedish Academy of Pharmaceutical Sciences) was held at Malmö

University and co-organised by the Center. Center members have been active presenting

their results on numerous occasions at national and international conferences and

workshops. Center activities have also been visible through e.g. articles in PS Public

Service Review European Union 19, a press release highlighting the 6th

annual workshop,

and Malmö University newsletters.







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3 List of Research Activities The research activities of the Center during 2010 may be described by the following

headlines. Projects are listed with partners and funding in paranthesis. Projects in bold are

funded by the center grant from KKs. In addition, we are also involved in three EU

Interreg programmes; ―Valorisation of knowledge intensive ideas in the South Baltic

area‖ (SB-VALOR), ―Öresund forum for innovation within nano-, bio- and medical

technology‖ (FinNBMT) and ―Öresund materials innovation community (Ö-MIC)‖.

3.1 Eucaryotic cell-surface interactions

1. Biomaterial 1(2), The influence of biochemical coat for implant bone

incorporation, The in vivo part (Promimic AB, funded by KKs-Biofilms)

2. Cell-to-bio-mimetic interface interactions (funded by EU Marie Curie Research

Training Networks)

3. Digital holography for cell studies (Phase Holographic Imaging AB, funded by

the Crafoord foundation, the Magnus Bergwall foundation and Mah)

4. Biological responses to photo-reactive hydrophilic nano-size structures (funded

by VR)

5. Hydrophilic and hydrophobic implant surfaces (funded by Vilhelm and Martina

Lundgren Foundation)

6. Histological and 3-dimensional analysis of laminin coated polished ceramic

implants (funded by Hjalmar Svensson Research Foundation)

7. The use of CaO as luting material and bone substitute (funded by VR)

8. Facilitation of soft tissue healing upon implant treatment in patients with

supressed healing ability (funded by Mah)

3.2 Molecular transport phenomena

9. Adjuvants for products used in agriculture (AkzoNobel Surface Chemistry

AB, funded by KKs-Biofilms)

10. Water – a crucial factor in regulating biomembrane permeability (Physical

Chemistry 1, LU, funded by FLÄK, LU)

11. Humectants and their mechanisms in skin (YKI AB, ACO Hud Nordic AB,

Eviderm Institute AB, funded by KKs)

12. Miniature biofuel cells for self-contained bio-devices: electron transfer in three-

dimensional nanobiostructures (funded by VR & EU)

13. Grinfeld surface instabilities (funded by VR)

14. Effect of a gradient in water chemical potential on buccal drug delivery (Food

technology, LU, funded by Mah)

3.3 Molecular interactions at biointerfaces

15. Mucoadhesion: Drug carrier interactions at biologically relevant interfaces

(Camurus AB, funded by KKs-Biofilms)

16. Bioassay: New concept for lipid-based surface coatings in bioassays

(EuroDiagnostica AB, funded by KKs-Biofilms)







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17. Biomaterial 2(2), The influence of biochemical coat for implant bone

incorporation, The in vitro part (Promimic AB, funded by KKs-Biofilms)

18. Development of biofuel cells for powering wireless transmission (Novosense

AB, funded by KKs-Biofilms)

19. Attachable Diagnostic Devices with Individualised Referencing (ADDIR)

(Galenica AB, funded by KKs-biofilms)

20. Symptomatic vs. asymptomatic atherosclerotic plaques (CRC, LU, funded in part

by Mah)

21. Biocompatibility of metals (Anordica AB & Arcam AB, funded by KKs)

22. Hydration of mucous gel (funded by Mah)

23. Topical hydrogen peroxide in wound healing (Bioglan AB, pilot project)

24. Membrane impedance spectroscopy as a tool to study skin barrier function and

variability (Galenica AB & Dermatology (LU), pilot project)

25. Development of novel multi-functional salivary substitutes for dry mouth

syndrome patients (funded by the Swedish Laryng Foundation)

26. Screening of phase behavior in the DOPS/DOPE/water system and effects on lipid

morphology from a decrease in water chemical potential (funded by CLRF)

3.4 Microbial biofilms

27. Milk Protein: Investigation of interactions between osteopontin and oral

biofilm bacteria (Arla Foods AB, funded by KKs-Biofilms)

28. Carrier: Investigation and modelling of convection in biofilms for different

carriers (AnoxKaldnes AB, funded KKs-Biofilms)

29. Probiotics: Effects of probiotic lactobacilli on biofilm formation and acid

tolerance (Biogaia AB, funded by KKs-Biofilms)

30. Catheters: Biofilm formation on Peritoneal Dialysis catheters (Gambro

Lundia AB, funded by KKs-Biofilms)

31. Biologically induced stress corrosion crack growth (Arcam AB, funded by

KKs-Biofilms)

32. Biofilm activity as a marker to identify patients at risk of caries –

mechanisms underlying microbial stress tolerance (funded by KKs-Biofilms)

33. Caries prevention with fluoridated milk – a prospective clinical and

microbiological study of root caries (funded by Swedish Patent Revenue

Foundation)

34. Biofilms on oral mucosal surfaces (funded by Swedish Dental Society)

35. Mucins and microbial biofilms – a symbiotic relationship for health (funded by

Mah and Crafoord Foundation)

36. Mucosal interactions as inducers of acid tolerance in oral microorganisms (funded

by Crafoord Foundation and Swedish Patent Revenue Foundation)

37. Activities of microbial biofilms on bioactive implant surfaces (funded by Mah)

38. The plasminogen activating system – interaction with microorganisms and a

potential risk marker (funded by Swedish Dental Society)







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4 Research highlights

4.1 Friction force spectroscopy for the study of the strength of protein layers

Protein layers can confer diverse properties on surfaces such as molecule-binding,

biocompatibility, or simply act as protective barriers against the surrounding medium.

Considering the vast number of applications where they are involved, these layers can be

subjected to multiple damage sources, including those of mechanical origin. Thus, the study of

the mechanical properties of protein layers can provide with useful information for i) a better

understanding of how the proteins interact both between themselves and with the underlying

substrate, but also for ii) their design so that their resistance against mechanical damage is

increased. During the last decade, the Atomic Force Microscope (AFM), where a sharp nm-sized

tip is used to probe surfaces, has emerged as a powerful tool to study mechanical properties of

protein layers. We have approached the study of these properties by a new methodology based on

the Friction Force Spectroscopy (FFS) operation mode of the AFM, which allows studying the

sample response to both normal and shear applied stresses. This methodology is based on the

continuous two-dimensional scanning of a surface while ramping the applied load force. For each

load force, a topography image of the scanned sample area and the average applied friction force

during the scan are recorded. Therefore, FFS allows the characterization of the layers with

friction-load curves, and with the characteristic topographies (like the initial rupture or the total

removal) that correspond to the different regimes of the scratching process. This technique has

been tested on β- and κ-casein monolayers. Caseins do not only act as natural emulsifiers in milk,

but are also used as emulsifiers or dispersants in many technological and industrial applications.

Our experiments showed that they can support pressures up to hundred of MPa before being

removed while still exhibiting a high frictional behaviour, supporting their good performance as

emulsifiers. Moreover, the technique was also proved to be able to study the dependence of the

cohesion of the layers with properties of the surrounding liquid medium such as pH and ionic

strength. Recent experiments on systems such as serum-proteins and saliva layers support the

wide applicability of the technique.

Figure 4.1. a) Schematic draw of a FFS measurement. b) and c) Topography images and friction-

load curve corresponding to the scratching of a β- -casein monolayer.

Sotres J, Svensson O and Arnebrant T., Friction force spectroscopy of β- and κ-casein monolayers. Langmuir 2011, 27(3), 981–992.







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4.2 Bioelectrocatalytic interfaces: Redox Enzymes Electronically Connected at Three-Dimensional Materials for Extracting Electrical Energy from Biofuels

Bioelectrocatalytic devices, such as biosensors, have proven to be useful in different areas of

applications including biomedicine. Current research in bioelectrochemistry is massively focused

on the improvement of biofuel cells. The last year we have aimed to design three-dimensional

(3D) conducting materials which, when loaded with redox enzymes, can constitute high power

biofuel cells. Conducting 3D materials were based on redox hydrogels [1], carbon [2] or gold [3]

nanoparticles, and nanostructured silica [4]. It was found that all these materials provide a

possibility to control the procedures of assembly of 3D electrodes. The work was devoted to tune

or adjust selectivity and optimize catalytic activity of the redox enzymes incorporated into the 3D

structures of these electrodes. Recently developed 3D electrodes of biofuel cells show at least 10

times higher current densities if compare with 2D electrodes. 3D cathodes developed in our

laboratory provide 20-400 µA/cm2 current densities. This means that a few micrometer thick

biofuel cell of approximately 1 cm2 area already now might power wireless biomedical devices.

Figure 4.2. An example of 3D electrode based on micro-/ nanostructured silica layer.

Relevant publications:

1. Design of a bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a specifically adapted Os-

complex containing redox polymer with entrapped Trametes hirsuta laccase. Ackermann, Y.; Guschin, D.A.; Eckhard, K.;

Shleev, S.; Schuhmann, W. Electrochemistry Communications, 2010, 12(5), 640-643. 2. Stable floating air diffusion biocathode based on direct electron transfer reactions between carbon particles and high redox

potential laccase. Shleev S.; Shumakovich G.; Morozova O.; Yaropolov A. Fuel Cells, 2010, 10(4), 726-733.

3. Laccase-gold nanoparticle assisted bioelectrocatalytic reduction of oxygen. Dagys, Marius; Haberska, Karolina; Shleev, Sergey; Arnebrant, Thomas; Kulys, Juozas; Ruzgas, Tautgirdas. Electrochemistry Communications, 2010, 12(7), 933-935.

4. Bioelectrochemical studies of azurin and laccase confined in three-dimensional chips based on gold-modified nano-/microstructured silicon. Ressine A., Vaz-Dominguez C., Fernandez V.M., De Lacey A.L., Laurell T., Ruzgas T., Shleev S.

Biosensors and Bioelectronics, 2010, 25(5), 1001-1007.

The following projects support the development of 3D biofuel cells:

1. Wireless self-powered biodevices: Function of nanowired multicentre redox enzymes and living cells", 2010-2012. Main

applicant: Sergey Shleev. The Swedish Research Council, project number: 621-2009-3266. 2. Bioelectrochemical 3D nanobiostructures in physiological liquids and cell based in-vitro platforms. 2009-2011. Main applicant:

Tautgirdas Ruzgas. The Swedish Research Council, project number: 2008-3713.

3. Three-dimensional nanobiostructure-based self-contained devices for biomedical application, 2009-2012. Coordinator: Sergey Shleev. EU FP7-NMP-2008-SMALL-2. Grant Agreement Number: 229255.







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4.3 Factors affecting transport of tebuconazole over leaf cuticle

The complex structure of plant cuticles constitutes the main barrier for fungicide uptake in leaves.

Consequently, the detailed underlying mechanism of action of adjuvants, often used to facilitate

fungicide permeation in leaves, is also complex and remains to be resolved. The overall aim of

this project is thus to obtain a better understanding of the mechanisms of action of specific

surfactants, in order to facilitate the design of better adjuvants for agricultural fungicides. We

have combined in vitro diffusion methodology (Franz cells) with sorption isotherms and

membrane impedance spectroscopy to evaluate the effect of two specific adjuvants (C10EO7 and

C8G1.6) on the bioavailability of tebuconazole as model active ingredient, using the adaxial side of

leafs from the model plant Clivia Miniata Regel as the membrane. The barrier properties of plant

leaves may respond to external factors, like changes in ambient relative humidity or gradients

imposed by individual formulations applied and therefore, ―inert‖ silicone membranes were

employed in parallel to plant membranes to distinguish between factors affecting the diffusion

coefficient in the membrane (Di) and the gradient in chemical potential (di/dz) over the

membrane. From the data given below it is evident that fungicide permeation over silicone

membrane is strictly dependent on the gradient in tebuconazole chemical potential (Figure 4.3.1),

while the prescence of surfactants has a strong impact on the diffusion coefficient of tebuconazole

in Clivia cuticle, resulting in up to four times higher permeability (Figure 4.3.2). C10EO7 is more

effective than C8G1.6 in promoting tebuconazole permeation through the Clivia cuticle, and

C10EO7 also has a more pronounced ability to decrease the extremely long lag-time. Moreover, the

Clivia cuticle can accommodate large amounts of tebuconazole and thereby act as a depot over

time. We have also shown using impedance spectroscopy that the barrier properties of Clivia

cuticle improves with maturation of the leaf.

Figure 4.3.1. Tebuconazole permeability over a

silicone membrane. Carrier: water, and water-

surfactant (4%) mixtures. ateb = 0.80.

Figure 4.3.2. Tebuconazole permeability over a

Clivia membrane. Carrier: water, and water-

surfactant (4%) mixtures. ateb = 0.80.

Factors Affecting Transport of Tebuconazole over Silicone Membrane and Leaf Cuticle, Fagerström A, Kocherbitov V, Lamberg P,

Bergström K, Westbye P, Ruzgas T and Engblom J. in 9th International Symposium on Adjuvants for Agrochemicals, ISAA Society; Baur P and Bonnet M Eds. August 2010, Pages 329-336 ISBN 978-90-815702

Effects of adjuvants on Tebuconazole leaf cuticle penetration, Fagerström et al, Manuscript to be submitted 2011.

Surfactant induced fluidization of plant leaf cuticle, Fagerström et al, Manuscript to be submitted 2011.







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4.4 Low levels of fluoride inhibit acid tolerance of plaque bacteria in vivo

Fluoride is used for prevention of dental caries mainly due to its potential to decrease solubility

and promote remineralisation of enamel and root dentin. In vitro studies show that fluoride also

affects the physiology of oral streptococci. However, data on the effect of fluoride on acid

tolerance in plaque in vivo is limited. The aim of this investigation was to study the effect of

fluoride on plaque acid tolerance and composition, lactic acid production, and the clinical effect

on re-mineralization of root caries lesions. The test group (F-group) consumed 200 ml of cow’s

milk supplemented with 5 mg/L NaF as a single dose once per day, the milk control group (M-

group) drank 200 ml of unsupplemented cow’s milk and a no-milk control group (C-group) did

not consume milk in this manner. The length of the study was 15 months. Dental plaque samples

were taken at baseline and after 15 months. The proportion of acid tolerant bacteria in plaque was

estimated using LIVE/DEAD® BacLightTM

staining after exposure to pH 3.5 for 2 hours. Lactic

acid production after glucose pulsing was measured enzymatically. The Electronic Caries

Monitor (ECM) was used to measure the electrical resistance of root surface lesions. Plaque from

subjects in the F-group showed a statistically significant decrease in plaque acid tolerance and a

significant increase in ECM values were found after 15 months compared to baseline indicating

re-mineralization. Lactic acid production from glucose was also lower in the F-group although

not statistically significant. This experimental clinical study shows that daily intake of fluoride

reduces plaque acid tolerance and lactic acid production in vivo and promote remineralization of

root caries lesions.

Figure 4.5. Bacteria in plaque stained with LIVE/DEAD® BacLightTM

staining. Green cells are

acid tolerant while red cells are not. Neilands J., L.G. Petersson, D. Beighton and G. Svensäter. Fluoride inhibits acid tolerance of root surface biofilms. Manuscript

DECREASED PLAQUE

ACID TOLERANCE

INCREASED PLAQUE

ACID TOLERANCE

BASELINE AFTER 15 MONTHS







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4.5 Peritoneal dialysis catheters show presence of bacteria without clinical signs of infection

Fifteen peritoneal dialysis catheters extracted from patients undergoing renal transplantation (i.e.

from patients with no clinical signs of infection) were investigated for the presence of bacteria

using microbiological culture and confocal laser scanning microscopy (CLSM). For reference,

two catheters from patients with infections were also investigated. The results showed 82% of the

catheters to be colonised by bacteria, although in a rather low numbers. The bacteria were

heterogeneously spread all over the catheter surface and several species often colonised the same

area (Figure 1). The major species found were Staphylococus epidermidis and Propionibacterium

acnes, but several others were also detected, including Micrococcus spp, Staphylococcus

lugdunensis, Staphylococcus warneri, Corynebacterium spp, Proteus mirabilis, Rothia

mucilaginosa, Streptococcus sanguis and Staphylococus aureus.

Figure 4.6. Species found on peritoneal dialysis catheters positive in the microbiological cultures.

Pihl M, Davies JR, Johansson A-C and Svensäter G. ―Occurrence of bacteria on catheters in patients undergoing peritoneal dialysis‖

Submitted to Journal of Medical Microbiology.







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4.6 Adhesion of Streptococcus oralis to titanium surfaces - effects of surface roughness and conditioning films

Initial healing is a critical phase in dental implant therapy and optimum surface roughness is one

of the key factors of importance for successful osseo-integration. Surfaces designed to promote

osteoblast activity may however also enhance bacterial adhesion and stimulate microbial activity

thus increasing the risk of peri-implant infections in the longer-term. The aim of this investigation

was to determine how surface roughness affects the adhesion of Streptococcus oralis (a species

frequently isolated from peri-implant infections) to titanium surfaces used in dental implants in

the presence of conditioning films derived from saliva and serum.

Titanium plates with average surface roughness (Sa) of 0,5 or 1,5 µm, uncoated or coated with

25% whole saliva or 5% human serum were exposed to exponential growth phase cells of

Streptococcus oralis (LA 11) in a flow-cell system for 2 h. After washing for 1h, the numbers of

adhered bacteria were assessed using confocal laser scanning microscopy (CLSM) after staining

with the Baclight Live/Dead kit (Figure 1). The mean percentage bacterial coverage on the

uncoated smooth surface (Sa = 0.5 µm) was 2±1% while that on the moderately rough surface (Sa

= 1.5 µm) was significantly greater (mean percentage coverage = 10.67 ± 0.88%, p < 0.01).

Figure 4.7. CLSM images showing biofilms formed on moderately rough titanium surfaces in (a)

the absence of a conditioning film or in the presence of (b) a saliva- or (c) and serum-derived

conditioning film.

For both the moderately rough and smooth surfaces, a conditioning film of saliva significantly

increased the adhesion of bacteria (p< 0.01) whereas bacterial binding in the presence of a serum-

derived film showed no differences to that on the control (uncoated) surface. These data suggest

that increased surface roughness, as well as the presence of salivary proteins, are important

determinants of the level of colonization by streptococci on oral implant surfaces.

Dorkhan M, Chávez de Paz M, Svensäter G and Davies JR. ―Adhesion of Streptococcus oralis to turned and blasted titanium surfaces - effects of saliva- and serum-derived pellicles‖. Manuscript







16

4.7 How to detect protease activity of dental biofilms in situ

Proteolytic bacteria export proteases to their immediate surroundings. The extracellular

proteolytic activity of these bacteria can thus be monitored through the addition of a protease

substrate to the culture medium. Casein is a substrate for the four major types of proteases (serine,

aspartic, cysteine and metalloproteases) and is thus a suitable substrate for screening protease

activity. Using fluorescein-labeled casein, protease activity can be detected. Proteolytic activity of

bacteria grown in biofilms has been visualized by confocal microscopy after addition of

fluorescein-labeled casein.

Figure 4.8. Proteolytic activity of bacteria in a clinical subgingival biofilm sample visualized as

green fluorescence.

Kinnby B, Wickström C & Svensäter G, Method development 2010







17

4.8 Selective adhesion and phenotypic changes in oral streptococci revealed through interaction with mucin-conditioned surfaces

The resident microflora of dental plaque changes as it matures over time. Different streptococcal

species are suggested to adhere and colonize at different time points in the maturation process,

where early colonizers have the ability to adhere and grow, using the host proteins as substrates

and late colonizers require other bacteria or their products to be able to adhere and grow. We

show here, that two streptococcal species, Streptococcus mitis biovar 2 and Streptococcus mutans,

display very different behaviors when introduced to surfaces conditioned with human salivary

mucin MUC5B, the major glycoprotein found in the mucus film covering all oral surfaces. Using

test surfaces conditioned with the salivary MUC5B mucin, S. mitis biovar 2 showed avid

adherence as well as a phenotypic shift towards more protease active cells. When introduced to

surfaces conditioned with other salivary proteins, this effect was not seen, suggesting a specific

interaction between S. mitis biovar 2 and the MUC5B molecule. However, when S. mutans was

studied under the same conditions, very few cells adhered to the conditioned surfaces, MUC5B or

the other salivary proteins, suggesting an inability of S. mutans to adhere to the natural occurring

salivary film components found in vivo. The same increase in protease active cells observed for S.

mitis biovar 2 was found in the S. mutans cells, although there was no difference between the two

substrates. This work clearly shows a specific difference between two streptococcal species in the

way they adapt to different environments, S. mitis biovar 2 being able to adhere and colonize a

salivary conditioning film, whereas S. mutans cannot.

Figure 4.9. Adhesion of S. mitis biovar 2 (A) and S. mutans UA159 (B) to surfaces coated with

salivary proteins, MUC5B or low-density salivary proteins. Bacteria were allowed to adhere to

coated or uncoated surfaces for 2 hours and attached cells were visualized in a CLSM after

BacLight™ LIVE/DEAD staining. * Differences are statistically significant (p < 0,05) using the

Mann-Whitney t-test.

Christian Kindblom, Gunnel Svensäter & Claes Wickström Salivary proteins influence phenotypic changes in Streptococcus mutans

and Streptococcus mitis biovar 2 biofilm cells – differences in adhesion and protease activity. To be submitted.







18

4.9 Spatial distribution of multiple species in complex biofilm communities

In nature, bacterial biofilm communities are highly organized structures composed of multiple

species that are believed to interact with each other in order to coexist. Synergistic coexistence of

bacteria in mixed biofilm communities is suggested to play a crucial role in development of

chronic infections as many species can become extremely resistant against antimicrobials and

host defences. To better understand the spatial structure of multiple species communities it is

necessary to visually characterize the distribution of individual species. We have developed an

automated in vitro system to study spatial distribution of bacteria in multi-species biofilm

communities. Our system is based in a combination of fluorescence in situ hybridization (FISH),

confocal laser scanning microscopy (CLSM), and digital image analysis. FISH probes targeting

the 16S rRNA gene are designed to verify the abundance and spatial location of microbial

community members. Overall, the knowledge gained by this method about distribution and

interactions in multi-species biofilms will facilitate the rapid analysis of microbial communities in

the sense of assessing changes in microbial populations as a function of time or environmental

conditions. Prospectively, this methodology can be applied in combination with specific

fluorescent markers targeting metabolic processes to allow the investigation of in situ

structure/function analysis of complex microbial communities.

A

B

Figure 4.10. Spatial distribution of two oral biofilm communities. (A) A four-species biofilm

community composed of clinical root canal isolates of Lactobacillus salivarius (red),

Streptococcus gordonii (green/yellow), Actinomyces naeslundii (blue) and Enterococcus faecalis

(violet). (B) A three-species biofilm community composed of supra-gingival isolates of

Lactobacillus fermentum (red), Streptococcus gordonii (green/yellow) and Actinomyces

naeslundii (blue). Bar = 10 µm.

Chavez de Paz L, 2010







19

4.10 On Ca2+ incorporation and nanoporosity of titanium surfaces and the effect on implant performance

Implants need to perform in three biological arenas: in relation to bone-tissue, soft-tissue, and

microbial biofilms. An implant should be properly osseointegrated and have a tight adaptation of

surrounding soft-tissues but it should at the same time not be prone for extensive biofilm

formation or be difficult to clean. More specifically we aimed for:

Bone: To evaluate the importance of anodic oxidation and Ca2+ incorporation/surface

chemistry of commercially pure titanium implants regarding osseointegration, and

whereas the chemical modification may compensate for a minimal surface roughness.

Oral mucosa: To evaluate the effect of sol-gel derived nanoporous TiO2 coating of

commercially pure titanium for the adaptation of oral mucosa.

Bacterial adhesion and biofilm formation: To investigate bacterial adhesion, as well as

biofilm formation and retention of oral bacteria in vitro on smooth and moderately rough,

anodized and Ca2+ incorporated, as well as, nanoporous surfaces.

Figure 4.10.1 Bone adhesion towards Ca ion

implanted surfaces, in trabecular and cortical

bone

Figure 4.10.2 Soft tissue adhesion

demonstrated with TEM

We have shown that surface chemistry/anodic oxidation and Ca2+ incorporation of titanium

surfaces may enhance the osseointegration and could possibly compensate for a minimal surface

roughness. Nanoporous TiO2 coating indicates some advantages in relation to unmodified

titanium regarding the sealing of oral mucosa. A tendency of increased biofilm accumulation of

oral bacteria in vitro was found for moderately rough (Sa 1-2 μm) blasted surfaces compared to

smooth ones (Sa <0.5 μm). Moderately rough surfaces, in addition, retained more bacteria after

mechanical removal of adhered biofilms compared to smooth. Nanoporosity or Ca2+

incorporation did not affect the bacterial adhesion or biofilm formation compared to turned

surfaces.

Increased bone contact to a Ca2+ incorporated oxidized c.p. titanium implant: an in vivo study in rabbit. Fröjd V, Franke-Stenport V,

Meirelles L, Wennerberg A. Int J Oral Maxillofac Surg 37:6 (2010) 561-6.

Importance of Ca2+ modifications for osseointegration of smooth and moderately rough anodized titanium implants – a removal torque and histological evaluation in rabbit. Fröjd V, Wennerberg A, Franke-Stenport V. Clin Impl Dent Rel Res 2010.

Nanoporous TiO2 thin film on titanium oral implants for enhanced human soft tissue adhesion - a histological evaluation in three

different levels of resolution. In situ analysis of biofilm formation on titanium surfaces Fröjd V, Chávez de Paz L, Andersson M, Wennerberg A, Davies J, Svensäter G. Submitted.

Microbial biofilm formation on smooth nanoporous TiO2 coated and anodized Ca2+ modified surfaces. Fröjd V, Linderbäck P,

Wennerberg A, Chávez de Paz L, Svensäter G, Davies J. Submitted.







20

4.11 Biologically induced stress corrosion as an indicator of residual stress.

In general, surface instabilities play an important role in industry. The applications are initial

stages of biologically induced corrosion. The examples from nuclear, petroleum and offshore

industry are numerous. Problems arise during manufacturing and in operation because of high

stresses and exposure to aggressive environment. As much as 25% of all accidents reported to the

Swedish Plant Inspectorate are claimed caused by stress corrosion. A large part of these can be

attributed to biological induced corrosion. A severe circumstance is that the cracks propagate at

very small loads. Occasionally the general status of the environment is sufficient to cause stress

corrosion, but in general the extreme local micro-environment under microbial colonies are

responsible for so called pitting and initiation of edge cracks that subsequently grow into the

structure.

Apart from the immediate advantage of more knowledge, several applications taking advantage of

the possibilities for non destructive testing of stresses could have been identified. The most

striking example is that inspection of a developing surface roughness has been proven to work as

a tool to discover high mechanical stresses and risk zones for corrosion cracks. Theoretical

studies of an amorphous material explain the mechanism behind the repeated branching of cracks

that is observed for cracks in aggressive environment. Numerically simulated crack growth was

performed using a moving boundary finite element formulation. The results show great agreement

with the experiments. An observed scatter in results in both experiments and in the numerical

simulations reflects an inherent perturbation sensitivity of environmentally assisted cracking.

Figure 4.14. Left) Typical initiation pattern of small pits resulting from an aggressive

environment. The pit density of pits reveal the mechanical stress in the structure. Right) Maximal

principal strain field surrounding a newly branched crack. The arrows indicate where the surface

straining equals the threshold strain.

On initiation of chemically assisted crack growth and crack propagation paths of branching cracks in polycarbonate, Hejman, Ulf,

2010, Licentiate Thesis, 77 pages, Media-Tryck AB, Lund Sweden Dissolution driven crack branching in polycarbonate, Hejman, Ulf, Bjerkén, Christina, Fatigue and Fracture of Engineering Materials

and Structures, 2010

Environmentally assisted initiation and growth of multiple surface cracks, Hejman, Ulf, Bjerkén, Christina, International Journal of Solids and Structures, 14-15, vol. 47, p. 1838-1846, 2010







21

5 PhD Theses Supervised by Center Members Theses defended:

1. Rickard Hägglund, LTH (Supervisors Per Ståhle, P Isaksson): ―Damage of paper

materials‖ (2009-2010)

2. Helena Tassids, LU. (Supervisors: Anette Gjörloff Wingren, HS and Pirkko Härkönen,

LU): ―Expression and function of the protein tyrosine phosphatases SHP-1a nd SHP-2 in

prostate cancer‖ (2005-2010).

3. Victoria Fröjd, Malmö University (Supervisors Ann Wennerberg, Gunnel Svensäter, Julia

Davies, Victoria Franke Stenport (GU) ―On Ca2+

incorporation and nanoporosity of

titanium surfaces and the effect on implant performance‖ (2008-2010)

Theses in progress:

4. Jildiz Hamit Eminovski, Malmö University (Supervisors Thomas Arnebrant and Krister

Eskilsson (Kemira AB)) "Interactions of adsorbed layers of carbohydrate containing

polymers - Saliva, mucins and bacterial surfaces" (2005- , Lic thesis defended 2009,

doctoral thesis will be defended sept 2011)

5. Ulf Hejman, Malmö University (Supervisors Per Ståhle, Christina Bjerkén):

"Biologically induced stress corrosion" (2005-, Lic thesis defended 2010, doctoral thesis

will be defended autumn 2011)

6. Adnan Safdar, Lunds University (Supervisors Liu-Ying Wei, Per Ståhle)

"Biocompatibility of ion beam melted materials" (2008-, Lic thesis defended Dec 2010,

doctoral thesis will be defended autumn of 2012

7. Maria Pihl, Malmö University (Supervisors Gunnel Svensäter, Bertil Kinnby, Thomas

Arnebrant) ―Biofilms on Peritoneal Dialysis Catheters‖ (2005-)

8. Alma Masic, Malmö University (Supervisors Per Ståhle, Johan Helsing) "Mathematical

modeling of biofilms" (2007-)

9. Sebastian Björklund, Lund University (Supervisors Emma Sparr (LU), Johan Engblom

(Mah) and Krister Thuresson (Camurus AB)): "Water - a crucial factor in regulating

biomembrane permeability" (2008-)

10. Anton Fagerström, Malmö University (Supervisors Johan Engblom, Vitaly Kocherbitov

and Karin Bergström (AkzoNobel)): ―Bioavailability of active ingredients used in

agriculture‖ (2009-)

11. Yana Znamenskaya, Malmö University (Supervisors Vitaly Kocherbitov and Johan

Engblom): ―Hydration of mucous gel‖ (2009-)

12. Christian Alfredsson Kindblom, Malmö University (Supervisors Gunnel Svensäter, Claes

Wickström, Madeleine Rohlin): ―Biofilm activity as a marker to identify patients at risk –

mechanisms underlying microbial stress tolerance‖ (2009-)

13. Marjan Dorkhan, Malmö University (Supervisors Julia Davies, Gunnel Svensäter, Ann

Wennerberg): ―Activities of microbial biofilms on bioactive implant surfaces‖ (2009-)

14. Kostas Bougas, Malmö University (Supervisors Ann Wennerberg, Pentti Tengvall GU),

Victoria Franke Stenport (GU)): ―Protein coat and bone incorporation‖ (2009-)

15. Lory Melin, Malmö University (Supervisors Ann Wennerberg, Martin Andersson

(Promimic AB): ―On the importance of nanometer structures for implant incorporation in

bone tissue‖ (2009-)

16. Magnus Falk, Malmö University (Supervisors Tautgirdas Ruzgas, Sergey Shleev):

―Three-dimensional nanobiostructure-based self-contained devices for biomedical

application‖ (2009-)







22

17. Jon Lind, LTH (Supervisors Ali Massih, Christina Bjerkén): ―Methods for evaluation of

evaluation of the hydride embrittlement of Ni-based super alloys‖ (2009-)

18. Tuerdi Maymaytilli, LTH (Supervisors Christina Bjerkén, Per Ståhle): Influence of

plastic deformation on the formation and growth of embritteling metal hydride's" (2009-)

19. Liselott Ellmarker-Löfquist, Malmö University (supervisors: Liselott Lindh, Björn

Söderfeldt): ‖ A Clinical and subjective evaluation of single implant treatments. A

retroscpective study.‖(2009-)

20. Mariko Hayashi, Malmö University (Supervisors: Liselott Lindh, Ann Wennerberg, Ryo

Jimbo): ―Biological Responses to Amphiphilic Nano-size Structures‖ (2010-)

21. Peter Lamberg, Malmö University (Supervisors: Tautgirdas Ruzgas, Thomas Arnebrant):

Three-dimensional (3D) bioelectrochemical systems assembled from nanoparticles

(2010-)

22. Cathrine Albér, Malmö University (Supervisors: Johan Engblom, Vitaly Kocherbitov,

Marie Lodén, Robert Corkery): ―Humectants and their effectson skin barrier function – a

mechanistic approach‖ (2010-)

23. Caitriona Ericsson, Lund University (Supervisor: Gunilla Nordin Fredrikson): ―The role

of IL-16 in atherosclerosis development‖ (2010-)

24. Polyxeni Mantani, Lund University (Supervisor: Gunilla Nordin Fredrikson):

―Atheroprotective humoral and T cell immune responses against oxidized LDL‖ (2010-)

6 Collaborative Partners of the Center

6.1 Industry collaborators

(* Partners within KKs-Biofilm grant)

1. ACO Hud Nordic AB

2. Akzo Nobel AB*

3. Anordica AB

4. AnoxKaldnes AB*

5. Arcam AB*

6. Arla Foods AB*

7. Biogaia AB*

8. Bioglan AB

9. Bioinvent International AB

10. Camurus AB*

11. Euro-Diagnostica AB*

12. Eviderm AB

13. Galenica AB

14. Gambro Lundia AB*

15. Genovis AB

16. InnoScandinavia AB

17. Medeon AB

18. Medicon Valley Alliance

19. Nares AB

20. Nobel Biocare AB

21. Novosense AB*

22. Novozymes A/S, Denmark

23. Phase Holographic Imaging AB

24. Promimic AB*

25. PVA-MV AG, Germany

26. QuNano AB

27. Quantumwise A/S, Denmark

28. Stora Enso AB

29. Studsvik Nuclear AB

30. TetraPak AB

31. Volvo Aero AB

32. YKI AB







23

6.2 Academic collaborators

Collaborations with other universities and research institutions in Sweden

1. Prof. em. Kåre Larsson, Camurus Lipid Research Foundation,, Lund

2. Prof. Gunnar Bergenholtz, Microbiology/Endodontics University of Gothenburgh

3. Prof. Per Claesson, The Royal Institute of Technology (KTH) and Surface Chemistry

Institute (YKI), Stockholm

4. Prof Gunnar Dahlén, Microbiology/Endodontics University of Gothenburgh

5. Prof. Lo Gorton, Biochemistry, Lund University

6. Prof. Christer Hansson, Dermatology, Lund University

7. Prof. Martin Malmsten, Pharmacy, Uppsala University

8. Prof. Jan Nilsson, CRC UMAS, Lund University

9. Prof. Tommy Nylander, Physical Chemistry 1, Lund University

10. Prof. Adrian Rennie, Physics, Uppsala University

11. Prof. Mark Rutland, The Royal Institute of Technology (KTH) and Surface Chemistry


12. Prof. Olle Söderman, Physical Chemistry 1, Lund University

13. Prof. Per Uvdal, MAX lab, Lund University

14. Prof. Martin Andersson, Dept Applied Chemistry, Chalmers university of Technology.

15. Prof. Pentti Tengvall, Dept Biomaterials, Sahlgrenska Academy, Göteborg University

16. Prof. Tomas Albrektsson, Dept Biomaterials, Sahlgrenska Academy, Göteborg

University

17. Prof Marie Wahlgren, Food Technology, LTH, Lund University

18. Prof. Ingegerd Johansson, Cariology, Umeå University

19. Prof. Artur Schmidtchen, Department of Clinical Sciences, Lund University

20. Prof. Swante Twetman, Specialist Clinic for Oral Health Care, Hallands Läns Landsting,

Halmstad

21. Prof Bengt-Göran Rosén, Dept. of Business and Engineering Halmstad University

22. Assoc. Prof. Lars Norlén, Cell- and Molecular biology, Karolinska Institute

23. Assoc. Prof. Viveka Alfredsson, Physical Chemistry 1, Lund University

24. Assoc. Prof. Ola Bergendorff, Dermatology, Lund University

25. Assoc. Prof. Emma Sparr, Physical Chemistry 1, Lund University

26. Assoc.Prof. Peter Siesjö, Department of Clinical Sciences, BMC, Lund University

27. Assoc. Prof.Eva Blomberg, The Royal Institute of Technology (KTH) and Surface

Chemistry Institute (YKI), Stockholm

28. Associate Professor Victoria Franke-Stenport, Dept Prosthodontics, Sahlgrenska

Academy, Göteborg University

29. Dr Yngve Cerenius, MAX lab, Lund University

30. Dr Adam Feiler, The Royal Institute of Technology (KTH) and Surface Chemistry


31. Dr Isabel Goncalves, CRC UMAS, Lund University

32. Dr Robert Corkery, The Royal Institute of Technology (KTH) and Surface Chemistry


33. Dr Anna Westerlund, Odontology, Gothenburg University

34. Dr. Ivan Maximov, Solid State Physics, Lund University

35. Dr Valera Veryazov, Theoretical Chemistry, Lund University

36. Dr Karina Persson, Odontology, Umeå University

37. Dr Jenny Liao Persson, Center for Molecular Pathology, Lund University







24

38. Per Rabe, ÖTL, Specialist Clinic for Oral Health Care, Hallands Läns Landsting,

Halmstad

39. Björn Svensson, ÖTL, Specialist docent, Clinic forOral Health Care, Örebro Läns

Landsting, Örebro

International collaborations

1. Prof. em. Ian Hamilton, University of Manitoba, Dept of Oral Biology, Winnipeg,

Canada

2. Prof. Robert Baier, University of Buffalo, Industry/University Cooperative Research

Center for Biosurfaces, Buffalo, USA

3. Prof. F. C. Shih, King Fei Sals University, Dubai

4. Prof. Leslie Banks-Sills, Cornell University, Ithaca, NY, USA and University of Tel

Aviv, Israel

5. Prof. Iwona Beech, University of Portsmouth, UK

6. Prof. Jan Sunner, University of Portsmouth, UK

7. Prof. David Beighton, Guy's Kings and St Thomas' Dental Institute, Joint Microbiology

Research Unit, London, UK

8. Prof. R. Singh, Indian Institute of Technology, Mumbai, India

9. Prof. Bent Sörensen, Denmark Technical University, Riso Labs, Denmark

10. Prof. R. K. Thomas, Oxford University, Dept of Physical Chemistry, and ISIS at RAL

(Neutron reflection facility), Oxford

11. Prof. Regine Willumeit, GKSS Research Centre, Geesthacht, Germany

12. Prof. Mark Hertzberg, University of Minnesota, Department of Microbiology,

Minneapolis, USA

13. Prof. A. Needleman, Brown university, Providence, USA

14. Prof. Jukka Meurman, Helsinki University Central Hospital, Dept of Oral and

Maxillofacial Diseases, Helsinki, Finland

15. Prof. Gordon Proctor, Kings College, London, UK

16. Prof. Christopher Exley, Keele University, UK

17. Prof. Takashe Sawase, Dept Prosthodontics, University of Nagasaki, Japan.

18. Prof. Wolfgang Schuhmann, Ruhr-Universität Bochum, Germany

19. Prof. Edmond Magner, University of Limerick, Ireland

20. Prof. Dietmar Haltrich, Universität für Bodenkultur Wien, Austria

21. Prof. Phil Bartlett, The University of Southampton, UK

22. Prof. Dennis Cvitkovitch, University of Toronto, Dept of Microbiology, Toronto, Canada

23. Prof. Jeannine Brady, University of Florida, Dept of Oral Biology, Gainesville, USA

24. Prof Kamal Mustafa, University of Bergen, Norway

25. ProfAlexander Toikka, Faculty of Chemistry, Saint Petersburg State University, Russia

26. Prof. Hermann Eberl, University of Guelph,Canada

27. Assoc. Prof. Marie Ranson, School of Biological Sciences, Scientific Director – Cancer,

Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong,

Australia

28. Assoc. Prof. Duncan Sutherland, Aarhus University, Denmark

29. Assoc. Prof. Pablo Galindo, Department of Oral surgery and Implant Dentistry, Granada

University

30. Dr Justas Barauskas, Institute of Biochemistry, Vilnius, Lithuania

31. Dr Michael Ortize, Caltech, USA

32. Dr Andrey Jivkov, Manchester University, UMIST, UK







25

33. Dr. Isaac Klapper, Montana State University , Center of Biofilms Research, Montana,

USA

34. Dr Juozas Kulys, Inst. of Biochemistry, Vilnius, Lithuania

35. Dr. Anne Meyer, University of Buffalo, Industry/University Cooperative Research Center

for Biosurfaces, Buffalo, USA

36. Dr Srikumar Banerjee, Indian Atomic Research Centre, India

37. Dr. G. Fragneto, ILL (Neutron reflection facility), Grenoble

38. Dr Adam Heller, University of Texas at Austin, TX, USA

39. Dr Kenneth Holmberg, Tekniska högskolan i Helsinki, Finland

40. Dr Rafael Taborisky, Denmark Technical University, Riso Labs, Denmark

41. Dr Alexander Yaropolov, Inst. of Biochemistry, Moscow

42. Dr. Sergei Lobov, School of Biological Sciences, University of Wollongong,

Wollongong, Australia

43. Dr. Miguel Alcalde, Consejo Superior de Investigaciones Científicas, Applied

Biocatalysis group, Spain

44. Dr. Antonio L. De Lacey, , Consejo Superior de Investigaciones Científicas,

Bioelectrocatalysis group, Spain

45. Dr. Donald Leech, National University of Ireland, Galway, Ireland

46. Dr. Paulo G. Coelho, Department of Biomimetics, New York University

47. Dr. Yuusuke Kozai, Department of Radiology, Kanagawa Dental University

48. Dr. Jelena Kosoric, Centre for Oral Growth and Development, Queen Mary’s School of

Medicine and Dentistry, University of London, UK

7 List of Publications of the Center from 2010 and Onwards

All titles listed under journal articles, review papers, and book chapters have been or are

subjected to peer review. Center publications for the period 2005-2010 can be found at

http://www.mah.se/biofilms.

7.1 Journal articles and invited review papers in journals

1. Ackermann, Y.; Guschin, D.A.; Eckhard, K.; Shleev, S.; Schuhmann, W., Design of a

bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a

specifically adapted Os-complex containing redox polymer with entrapped Trametes

hirsuta laccase. Electrochemistry Communications, 2010, 12(5), 640-643.

2. Bjerken C, Ortiz M (2010) "Evolution of anodic stress corrosion cracking in a coated

material" INTERNATIONAL JOURNAL OF FRACTURE, 165, 2, p. 211-221

3. Bjerkén, Christina (2010) "The influence of biaxial loading on branching of a dissolution

driven stress corrosion crack" Engineering Fracture Mechanics 11, vol. 77, p. 1989-1997

4. Björkbacka H, Lavant EH, Fredrikson GN, Melander O, Berglund G, Carlson JA and

Nilsson J. Weak associations between human leukocyte antigen (HLA) genotype and

acute myocardial infarction. Journal of Internal Medicine, 268:1 (2010) 50-58

5. Björklund S, Engblom J, Thuresson K and Sparr E, A water gradient can be used to

regulate drug transport across skin, J Control Release, 143:2 (2010) 191-200

6. Chávez de Paz LE, Bergenholtz G, Svensäter G. The effects of antimicrobials on

endodontic biofilm bacteria. J Endod 2010;36:70-7.







26

7. Coelho PG, Granato R, Marin C, Jimbo R, Lin S, Witek L, Suzuki M, Bonfante EA.

Effect of Si Addition on Ca- and P- Impregnated Implant Surfaces with Nanometer Scale

Roughness. An Experimental Study in Dogs. Clin Oral Impl Res; in press

8. Coman, V.; Ludwig, R.; Harreither, W.; Haltrich, D.; Gorton, L.; Ruzgas, T.; Shleev, S.,

A direct electron transfer-based glucose/oxygen biofuel cell operating in human serum.

Fuel Cells, 2010, 10(1), 9-16.

9. Dagys, Marius; Haberska, Karolina; Shleev, Sergey; Arnebrant, Thomas; Kulys, Juozas;

Ruzgas, Tautgirdas., Laccase-gold nanoparticle assisted bioelectrocatalytic reduction of

oxygen. Electrochemistry Communications, 2010, 12(7), 933-935.

10. Dohan Ehrenfest DM, Lemo N, Jimbo R, Sammartino G. Selecting a relevant animal

model for testing thein vivo effects of Choukroun’s Platelet-Rich Fibrin (PRF) : rabbit

tricks and traps. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 110(4):413-6.

11. Dunér P; To F, Berg K, Alm R, Björkbacka H, Engelbertsen D, Fredrikson GN, Nilsson

J, Bengtsson E. Immune responses against aldehyde-modified laminin accelerate

atherosclerosis in Apoe-/- mice. Atherosclerosis, Oct;212(2):457-65, 2010.

12. Eliasson A, Wennerberg A, Johansson A, Ortorp A, Jemt T. The Precision of Fit of

Milled Titanium Implant Frameworks (I-Bridge(R)) in the Edentulous Jaw. Clin Implant

Dent Relat Res. 2010;12:81-90

13. Exley C, Siesjö P and Eriksson H. The Immunobiology of Aluminium Adjuvants: How

do they really work? Trends in Immunology, 31 (2010) 103-109

14. Fransson H, Petersson K., Davies JR. Dentine sialoprotein and Collagen I expression

after experimental pulp capping in humans using Emdogain® Gel. Int Endod J 2011, 44,

259–267

15. Fredrikson GN, Nilsson J, Björkbacka H. Autoimmunitet mot blodfett – kan ge både

skydd och sjukdom. Incitament, 19(7):579-583, 2010.

16. Fröjd V, Wennerberg A, Franke-Stenport V. Importance of Ca2+ modifications for

osseointegration of smooth and moderately rough anodized titanium implants-A removal

torque and histological evaluation in rabbits. Clin Implant Dent Relat Res 2010;26 E pub

ahead of print.

17. Halthur TJ, Arnebrant T, Macakova L, Feiler A. Sequential Adsorption of Bovine Mucin

(BSM) and Lactoperoxidase (LPO) to various substrates studied with Quartz Crystal

Microbalance with Dissipation (QCM-D). Langmuir 26:7 (2010) 4901-4908

18. Hamit-Eminovski J, Eskilsson K and Arnebrant T, An ellipsometry study on the effect of

aluminium chloride and ferric chloride formulations on mucin layers adsorbed at

hydrophobic surfaces. Biofouling, 26 (2010), 511-518

19. Hamit-Eminovski J, Eskilsson K and Arnebrant T, Change in surface properties of

Microthrix parvicella upon addition of polyaluminium chloride as characterized by

atomic force microscopy, Biofouling, 26 (2010), 323-331

20. Hejman, Ulf, Bjerkén, Christina (2010) "Dissolution driven crack branching in

polycarbonate" Fatigue and Fracture of Engineering Materials and Structures, In Press

21. Hejman, Ulf, Bjerkén, Christina (2010) "Environmentally assisted initiation and growth

of multiple surface cracks" International Journal of Solids and Structures 14-15, vol. 47,

p. 1838-1846

22. Hjalmarsson L, Smedberg JI, Wennerberg A. Material degradation in implant-retained

cobalt-chrome and titanium framworks. J Oral Rehabilitation 2010;29 E-PUB ahead of

print.

23. Jalali, Y., Lindh, L. A randomized prospective clinical evaluation of two desensitizing

agents on cervical dentine sensitivity: A pilot study. Swed Dent J. 34: 79-86, 2010.







27

24. Jimbo R, Ivarsson M, Koskela A, Sul YT, Johansson CB. Protein adsorption to surface

chemistry and crystal structure modification of titanium surfaces. J Oral Maxillofac Res

2010; 1(3):e3.

25. Johansson CB, Jimbo R, Steffenson P. Ex Vivo and In Vivo Biomechanical Test of

Implant Attachment to Various Materials. Introduction of a New User-friendly Removal

Torque Equipment. Clin Implant Dent Relat Res 2010; In press

26. Karlsson C, Ahrné S, Molin G, Berggren A, Palmquist I, Fredrikson GN, and Jeppsson B.

Probiotic therapy to men with incipient arteriosclerosis initiates increased bacterial

diversity in colon: a randomized controlled trial. Atherosclerosis, 208 (2010) 228-233

27. Klingenberg R, Lebens M, Hermansson A, Fredrikson GN, Strodthoff D, Rudling M,

Ketelhuth D, Gerdes N, Holmgren J, Nilsson J and Hansson GK. Intranasal immunization

with an apoB-100 fusion protein induces antigen-specific regulatory T cells and reduces

atherosclerosis. Arterioscler Thromb Vasc Biol, 30(5):946-52, 2010.

28. Kocherbitov V and Alfredsson V. Assessment of porosities of SBA-15 and MCM-41

using water sorption calorimetry. Accepted for publication in Langmuir

29. Kocherbitov V, Ulvenlund S, Briggner L-E, Kober M, Arnebrant T. Hydration of a

natural polyelectrolyte Xanthan Gum: comparison with non-ionic carbohydrates,

Carbohydrate Polymers 82 (2010) 284-290

30. Kocherbitov, V.; Arnebrant, T. Hydration of lysozyme: the protein-protein interface and

the enthalpy-entropy compensation. Langmuir (2010), 26(6), 3918-3922.

31. Kolbus D, Ramos O, Olofsson KE, Persson J, Wigren M, Björkbacka H, Fredrikson GN

and Nilsson J. CD8+ T cell activation predominate early immune responses to

hypercholesterolemia in Apoe-/- mice. BMC Immunol, 2010 Dec 2;11(1):58. [Epub

ahead of print].

32. Kolbus D, Wigren M, Ljungcrantz I, Söderberg I, Björkbacka H, Nilsson J and

Fredrikson GN. Immunization with cationized BSA inhibits progression of disease in

Apobec-1/LDL receptor deficient mice with manifest atherosclerosis. Immunobiology, in

press.

33. Masic A, Bengtsson J, Overgaard NC, Christensson M, Heyden A. Measuring and

modeling the oxygen profile in a nitrifying Moving Bed Biofilm Reactor. Mathematical

Biosciences, 227:1 (2010) 1-11

34. Melin Svanborg L, Hoffman M, Andersson M, Currie F, Kjellin P, Wennerberg A. The

effect of hydroxyapatite nanocrystals on early bone formation surrounding dental

implants. Int J Oral Maxillofac Surg. In press

35. Nilsson J and Fredrikson GN. The B cell in atherosclerosis - teaming up with the bad

guys? Clin Chem, Dec;56(12):1789-91, 2010.

36. Nilsson-Berglund LM, Zetterqvist AV, Nilsson-Ohman J, Sigvardsson M, González Bosc

LV, Smith ML, Salehi A, Agardh E, Fredrikson GN, Agardh CD, Nilsson J, Wamhoff

BR, Hultgårdh-Nilsson A, Gomez MF. Nuclear factor of activated T cells regulates

osteopontin expression in arterial smooth muscle in response to diabetes-induced

hyperglycemia. Arterioscler Thromb Vasc Biol, 30(2):218-24, 2010.

37. Ono D, Jimbo R, Kawachi G, Ioku K, Ikeda T, Sawase T. Lateral bone augmentation

with a newly developed beta-tri-calcium phosphate block: an experimental study in the

rabbit mandible. Clin Oral Impl Res; In press

38. Pedersen TX, Binder CJ, Fredrikson GN, Nilsson J, Bro S, Nielsen LB. The pro-

inflammatory effect of uremia overrules the anti-atherogenic potential of immunization

with oxidized LDL in apoE-/- mice. Nephrol Dial Transplant, Aug;25(8):2486-91, 2010.

http://www.ncbi.nlm.nih.gov/pubmed/19965778









28

39. Pihl M, Chávez de Paz LE, Schmidtchen A, Svensäter G, Davies JR. Effects of clinical

isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation.

FEMS Immunol Med Microbiol 2010;59:504-12.

40. Pihl M, Davies JR, Chávez de Paz LE, Svensäter G. Differential effects of Pseudomonas

aeruginosa on biofilm formation by different strains of Staphylococcus epidermidis.

FEMS Immunol Med Microbiol 2010;59:439-46.

41. Ressine A, Vaz-Dominguez C, Fernandez VM, De Lacey AL, Laurell T, Ruzgas T,

Shleev S. Bioelectrochemical studies of azurin and laccase confined in three-dimensional

chips based on gold-modified nano-/microstructured silicon. Biosensors and

Bioelectronics, 25:5 (2010) 1001-1007

42. Santos O, Lindh L, Halthur T and Arnebrant T, Adsorption from saliva to silica and

hydroxyapatite surfaces and elution of salivary films by SDS and delmopinol, Biofouling,

26 (2010), 697-710

43. Shleev S.; Shumakovich G.; Morozova O.; Yaropolov A., Stable floating air diffusion

biocathode based on direct electron transfer reactions between carbon particles and high

redox potential laccase. Fuel Cells, 2010, 10(4), 726-733.

44. Sotres J, Svensson O and Arnebrant T., Friction force spectroscopy of β- and κ-casein

monolayers. Langmuir 2011, 27(3), 981–992.

45. Stahle, P. (2010) "Foreword of the KB Broberg symposium issue" International Journal

of Fracture, 165(2): p. 139-139

46. Stahle, P., Barenblatt, G.I., and Rice, J.R. (2010) Biography of Professor Knut Bertram

Broberg" International Journal of Fracture, 165(2): p. 141-148

47. Stahle, P., Singh, R.N., and Banerjee, S. (2010) "Spontaneous fracture of growing

precipitates with large misfit strain" International Journal of Fracture, 165(2): p. 189-197

48. Stollenwerk MM, Svensson O, Schiopu A, Jansson B, Arnebrant T, and Fredrikson GN.

Adsorption of low density lipoprotein, its oxidation and subsequent binding of specific

recombinant antibodies – an in situ ellipsometric study. Biochimica et Biophysica Acta

(BBA) -General Subjects 2011, 1810(2), 211-217

49. Strietzel FP, Lafaurie GI, Bautista Mendoza GR, Alajbeg I, Pejda S, Vuletić L, Mantilla

R, Falcão DP, Leal SC, Barreto Bezerra AC, Tran SD, Ménard HA, Kimoto S, Pan S,

Martín-Granizo RA, Maniegas Lozano ML, Zunt SL, Krushinski CA, Melilli D, Campisi

G, Paderni C, Dolce S, Yepes JF, Lindh L, Koray M, Mumcu G, Elad S, Zeevi I, Aldape

Barrios BC, López Sánchez RM, Beiski BZ, Wolff A, Konttinen YT. Efficacy and safety

of an intraoral electrostimulation device for xerostomia relief: a multicenter randomized

trial. Arthritis & Reumatism. 63 (2010) 180-190

50. Svanborg LM, Andersson M, Wennerberg A. Surface Characterization of Commercial

Oral Implants on the nanometer level. J Biomed Mater Res B Appl Biomater. 2010;

92:462-469.

51. Svensson O and Arnebrant T, Adsorption of serum albumin on silica – The influence of

surface cleaning procedures. Journal of Colloid and Interface Science 2010, 344(1), 44-

47.

52. Svensson O and Arnebrant T, Mucin layers and Multilayers. Current Opinion in Colloid

& Interface Science 2010, 15(6), 395-405.

53. Svensson O, Halthur T, Sjödin T and Arnebrant T, The adsorption of delmopinol at the

solid liquid interface – the role of the acid base equilibrium. Journal of Colloid and

Interface Science 2010, 350, 275–281.

54. Tassidis, H., Culig Z., Gjörloff Wingren, A, Härkönen, P. Role of protein tyrosine

phosphatase SHP-1 in interleukin-6 regulation of prostate cancer. The Prostate 70 (2010)

1491-500.







29

55. Wennerberg A, Albrektsson T. Current Challenges in Successful Rehabilitation with Oral

Implants. J Oral Rehabil 2010 Oct 25 Epub ahead of print

56. Wennerberg A, Albrektsson T. On implant surfaces: a review of current knowledge and

opinions. Int J Oral Maxillofac Implants 25 (2010)63-74

57. Wigren M, Kolbus D, Dunér P, Ljungcrantz I, Söderberg I, Björkbacka H, Fredrikson

GN, Nilsson J. Evidence for a role of regulatory T cells in mediating the athero-protective

effect of apolipoprotein B peptide vaccine. J Int Med, 2010 Nov 3. doi: 10.1111/j.1365-

2796.2010.02311.x. [Epub ahead of print]

58. Zhao M, Wigren M, Dunér P, Bengtsson D, Olofsson K, Björkbacka H, Nilsson J,

Fredrikson GN.

Journal of Immunology, 184:5 (2010) 2253-2260

59. Östman PO, Wennerberg A, Albrektsson T. Immediate occlusal loading of NanoTite

PREVAIL implants: a prospective 1-year clinical and radiographic study. Clin Implant

Dent Relat Res. 12:1 (2010) 39-47

Submitted:

60. Chávez de Paz LE, Davies J, Svensäter G. Fluorescence in situ hybridization for

intracellular mRNA detection in adherent bacteria. Appl Environ Microbiol, submitted

61. Fröjd V, Chàvez de Paz L, Andersson M, Wennerberg A, Davies JR, Svensäter G. In situ

analysis of multi-species biofilm formation on customized titanium surfaces. Submitted

62. Fröjd V, Linderbäck P, Wennerberg A, Svensäter G, Davies JR. Microbial biofilm

formation on smooth nanoporous titanium dioxide coated and anodized calcium ion

modified titanium surfaces. (Submitted)

63. Jimbo R, Sotres J, Johansson C, Breding K, Currie F, Wennerberg A. The biological

responses to three different nanostructures applied on smooth implant surfaces. Clinical

Oral Implant Res. Submitted

64. Leisnert L, Karlsson M, Franklin I, Lindh L, Wretlind K., Improving teamwork between

students from two professional centers in dental education. Eur J Dent Educ. Submitted

65. Lindh L, Santos O, Svendsen IE, Sotres J, Breding K, Wennerberg A, Arnebrant T.

Adsorption of plasma proteins onto hydroxyapatite surfaces. Submitted

66. Pihl M, Arvidsson A, Skepö M, Svensäter G, Davies JR. Biofilm formation by

Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of

extracellular products from Pseudomonas aeruginosa. Submitted

67. Pihl M, Davies JR, Johansson AC, Svensäter G. Microbial biofilms in patients with

peritoneal dialysis catheters. (submitted)

Proceeding papers:

68. Fagerström A, Kocherbitov V, Lamberg P, Bergström K, Westbye P, Ruzgas T and

Engblom J. Factors Affecting Transport of Tebuconazole over Silicone Membrane and

Leaf Cuticle; in 9th International Symposium on Adjuvants for Agrochemicals, ISAA

Society; Baur P and Bonnet M Eds.; August 2010, Pages 329-336 ISBN 978-90-815702

69. Safdar A, Wei Liu-Ying, Snis A, Lai Z, ―Evaluations of microstructural development in

electron beam melted Ti-6Al-4V‖, Proc. MSE Conference 2010, 24-26 Aug, 2010,

Darmstadt Germany.

70. Sparr, Emma; Björklund, Sebastian; Engblom, Johan; Thuresson, Krister, A water

gradient can be used to regulate drug transport across skin - A responding membrane,

Biophysical Journal, Volume 98, Issue 3, Supplement 1, January 2010, Page 627a







30

7.2 Books and book chapters

1. Albrektsson T, Wennerberg A. The science of osseointegration. In; Prosthodontic

Treatment for Edentulous Patients, ed 13. Submitted 2010

2. Alm K, Cirenajwis H, Gisselsson L, Gjörloff Wingren A, Janicke B, Mölder A, Oredsson

S, Persson J. Digital holography and cell studies. In press, 2010

3. Gjörloff Wingren A, et al. Digital holographic microscopy - innovative and non-

destructive analysis of living cells. Microscopy Book Series - Volume # 4: ―Microscopy:

Science, Technology, Applications and Education‖ Release date: Autumn 2010

4. Julia R. Davies, Claes Wickström and David J. Thornton. Gel-forming and cell-

associated mucins – preparation for structural and functional studies in Methods in

Molecular Biology – Edited by McGuckin and Thornton. In press 2010

5. Winning TA, Skinner VJ, Kinnell A, Townsend, GC, Svensäter G, Rohlin M, Davies, JR.

The influence of two PBL curricula contexts on students’ understandings of PBL,

approaches to learning and outcome in Researching Problem-based Learning in Clinical

Education: The Next Generation. Edited by Bridges S, McGrath C, Whitehill T. In press

2010.

7.3 Popular articles

1. El-Schish Z, Mölder A, Sebesta M, Gjörloff Wingren A. Det digitala holografiska

mikroskopet – innovativ teknik för analys av levande celler. 2010, Bioingenjören

(Norge).

2. Engblom J, Interfaces in biomedicine PS Public service review European Union 19,

PSCA Int. Ltd 2010, 367

3. Engblom J, Interfacing the future, PS Public service review European Union 19, PSCA

Int. Ltd 2010, 366

4. Fridberg M, Tassidis H, Gjörloff Wingren A . PTPN7 (protein tyrosine phosphatase, non-

receptor type 7). Atlas Genet Cytogenet Oncol Haematol. January 2010

5. Reportage i Mun & Hals (tidning för mun- och halscancerförbundets medlemmar), 2010,

nr 2:8 om forskningsprojekt om saliversättningsmedel.

8 Visitors and Seminars at the Center 1. Prof Mark Herzberg, University of Minneapolis, USA, Seminar: ―Characterization of

hydrogen peroxide-induced DNA release by streptococci‖ 2010-06-17—07-03

2. Prof Charles Shuler, University of British Columbia, Canada, Seminar: ―Visions and

strategies in dental education‖ 2010-11-10—13

3. Prof Arunas Ramanavicius, Faculty of Chemistry, Vilnius University, Vilnius, Lithuania

4. Dr Almyra Ramanaviciene, Faculty of Chemistry, Vilnius University, Vilnius, Lithuania

5. Domhnall Mac Aodha, PhD student at National University of Ireland, Galway, Ireland,

2010-10-04--15

6. Marius Dagys, PhD student at the Institute of Biochemistry, Vilnius, Lithuania, 2010-08-

01--11-30.

7. Vida Krikstolaityte, PhD student at the Faculty of Chemistry, Vilnius University, Vilnius,

Lithuania, 2010-05-05--10-15.

8. Diana Mate, PhD student from Instituto de Catálisis y Petroleoquímica, CSIC, Madrid,

Spain 2010-08-01—09-30







31

9. Asta Makaraviciute, MSc-student at the Faculty of Chemistry, Vilnius University,

Vilnius, Lithuania.

10. Alexandra Pulyalina, PhD student at Faculty of chemistry, St Petersburg University,

Russia, 2010-01-06--02-05

11. Marija Jankunec, PhD student at Institue of Biochmistry, Vilnius, Lithuania, 2010-05-25-

-07-05 & 2010-09-15--12-15

12. Prof Per-Olof Glantz, Faculty of Odontology, Malmö University:Seminar: ―From

forbidden to high priority‖ 2010-10-13

13. Prof Göran Lundborg, Hand Surgery, Lund University, Seminar: ―Hand to brain in

research and development‖ 2010-10-14

14. Prof Tomas Albrektsson, Biomaterial Research, University of Gothenburg, Seminar: ―On

survival and success of oral implants‖ 2010-10-14

15. Prof Tommy Nylander, Physical Chemistry, Lund University, Seminar: ―On the

interaction of drug delivery vehicles with model biomembranes‖ 2010-10-14

16. Carin Daal, Region Skåne, Seminar: ―The innovation system in Skåne – what use can a

researcher have of it?‖ 2010-10-15

17. Klementina Österberg, GU Holding, Seminar: ―Investing in making a profitable

university business‖ 2010-10-15

18. Dr Evy Lundgren-Åkerlund, Ideon Bioincubator, Seminar: ―From adhesion to business‖

2010-10-15

19. Dr Jeanette Sundberg, PULS AB, Seminar: ―What makes an innovation successful?‖

2010-10-15

20. Drs Karin Bryskhe & Anna Stenstam, Colloidal Resource AB, Seminar: ‖ Business

windows at the intersection of academia and business‖ 2010-10-15

21. Jonas Gulliksson, Advokatbyrån Gulliksson, Seminar: ‖ IP strategies‖ 2010-10-15

22. Ashkan Pouya, Serendipity Innovations AB, Seminar: ―The Creative Dance‖ 2010-10-15

23. Eva Jensen, Advokatbyrån Lindahls, Seminar: ― Agreeing on intellectual property rights‖

24. Takashe Sawase, Nagasaki University, Japan

25. Humbeto Osvaldo Schwartz Filho, PhD-student at UNESP, Sao Paulo State University,

Brazil, (11 months)

26. Dr Lana Karlmark, Läkemedelsverket, Uppsala, Seminar: ―Myndighetens syn på CRO-

verksamhet‖ 2010-05-27

27. Dr Torbjörn Larsson, Medivir, Huddinge, Seminar: ―Samarbetet med CRO - Ur

uppdragsgivarens synvinkel‖ 2010-05-27

28. Dr Lars Wannerberger, Galenica AB, Malmö, Seminar: ―Samarbetet med CRO - Ur

uppdragstagarens synvinkel‖

29. Odd Swarting, Setterwalls advokatbyrå, Stockholm, Seminar: ―Juridiska aspekter och

avtal vid anlitande av CRO‖ 2010-05-27

30. Maria Stenbäck, Awapatent, Malmö, Seminar: ―Patentperspektivet – Vad bör man tänka

på?‖ 2010-05-27

31. Prof Per Jönsson, School of Technology, Malmö University, 2010-12-14

32. Assoc. Prof Yuanji Cheng, School of Technology, Malmö University, 2010-12-14

Internal seminars at the Center

33. Vitaly Kocherbitov ―Hydration of proteins‖ 2010-01-27

34. Yana Znamenskaya ―Effect of hydration on the structural properties of mucous gel‖

2010-02-09

35. Anna Gustafsson "Some aspects on immunomodulation by cationic peptides on bacterial

toxins" 2010-03-04







32

36. Varvara Petrova ―Chemical composition of the epicuticular wax layers of Clivia miniata

leaves‖ 2010-03-09

37. Helena Tassidis "Expression and function of the protein tyrosine phosphatases SHP-1 and

SHP-2 in prostate cancer" 2010-03-10

38. Emmy Nilsson and Anette Gjörloff Wingren "Interfacing antibody-based microarrays and

digital holography enables detection of cell death-induced lymphocytic cell lines" 2010-

04-07

39. Javier Sotres ― Friction and wear of adsorbed casein layers studied by AFM‖ 2010-04-13

40. Vida Krikstolaityte ―Assembly and catalytic properties of nanoparticles and enzymes‖

2010-04-21

41. Gabriella Sinkiewicz ―Påverkan av munhålans bakterieflora i en frisk population efter

tillförsel av Lactobacillus reuteri via tuggummi" 2010-05-05

42. Magnus Falk ‖Three-dimensional nanobiostructure-based biofule cells for biomedical

applications‖ 2010-05-11

43. Maria Stollenwerk och Anette Gjörloff Wingren ―Pedagogiskt seminarium om

bedömning‖ 2010-05-19

44. Vida Krikstolaityte ―Evaluation of (1) gold nanoparticle/laccase assembly at different

ionic strengths by using QCM-D and (2) bioelectrocatalysis of oxygen using LSV‖ 2010-

09-14

45. Diana Maté ―Laboratory Evolution of a High Redox Potential Laccase‖, 2010-09-14

46. Alejandro Barrantes ―"Interactions between DNA and Alzheimer's disease amyloid

peptides, and MAP tau, detected by Surface Plasmon Resonance" 2010-10-06.

47. Lina Pedersen ―Effect of a gradient in water chemical potential on buccal drug delivery‖

2010-10-12

48. Jovice Boon Sing Ng ―Mesoporous spheres as model materials for molecular transport

studies‖ 2010-10-20

49. Julia Hedlund ―z-LAB, an new and compact impedance measurement instrument for

sensitive surface analyses‖ 2010-11-03

50. Yana Znamenskaya ―Effect of hydration on the structural properties of mucin‖ 2010-11-

09

51. Pontus Dunér ―Immune responses against aldehyde-modified laminin accelerate

atherosclerosis in ApoE deficient mice‖ 2010-11-17

52. Maria Wigren ―MHC class II deficiency increases atherosclerosis in apoE-/- mice‖ 2010-

12-01

53. Anton Fagerström ―Factors affecting transport of tebuconazole over silicone and leaf

cuticle‖ 2010-12-07

54. Viktor Andoralov ''Bioelectrocatalytic bechaviour of Octaheme Nitride Reductase from

Thioalkalivibrio paradoxus'' 2010-12-15

9 Workshops and Conferences Organized by the Center During 2010 the Center has launched the 6

th Annual workshop as a joint venture with the

EU project ―Valorisation of knowledge intensive ideas in the South Baltic area‖ (SB-

VALOR). ,Focus this year was ―Biomaterials - From Fundamentals to Market

Application‖ and the event attracted 110 participants from different universities as well as

industries, innovation agencies, solicitor’s offices and risk capitalists The workshop was







33

preceeded by a PhD-student day with Prof. Per-Olof Glantz, former Vice-Chancellor of

Malmö University, and Prof. Tommy Nylander, Lund University as mentors.

The Center has also launched a ―Members day‖ for sharing progress in the different

projects with all partners, and coorganized a workshop on ―Choice of and collaboration

with CRO´s in pharmaceutical development‖ at Malmö University, launched by the

Swedish Academy of Pharmaceutical Sciences. Ann Wennerberg has been responsible

for arranging a 2-week workshop within the National Research School of Odontology;

―Biomaterials in medicine‖, together with BIOSUM, Gothenburg. Liselott Lindh

arranged a salivary symposium together with Guy Carpenter (London) at IADR in

Barcelona. Johan Engblom arranged a kick-off meeting for the new project ―Humectants

and their effects on skin barrier function – a mechanistic approach‖, funded by KKs

2010-2014.

Our internal seminars were organised by Tautgirdas Ruzgas and Anette Gjörloff

Wingren, and Gunilla Nordin Fredrikson was responsible for the seminars at Clinical

Research Center, Lund University

10 Contributions to and Participation in Conferences and Workshops

10.1 Oral presentations 2010

International

1. Fagerström A, Kocherbitov V, Lamberg P, Bergström K, Westbye P, Ruzgas T,

Engblom J. ―Factors affecting transport of tebuconazole over silicone membrane and

leaf cuticle‖, 9th international symposium on Adjuvants for Agrochemicals (ISAA 2010)

August 16-20, 2010

2. Fröjd V,Svensäter G, Andersson M, Wennerberg A, Chàvez de Paz L. In situ analysisof

biofilm formationon titanium surfaces. IADR, 88th general session, Barcelona, Spain,

July14-17, 2010.

3. Kocherbitov V and Wadsö L. Application of Sorption Calorimetry for Studies of

Hydration of Polymers, POLYSOLVAT-8 , 8th International IUPAC Conference

Polymer-Solvent Complexes & Intercalates, 5-8 July 2010, Strasbourg, France

4. Krikstolaityte V, Dagys M, Kulys J, Ramanavicius A, Arnebrant T, Shleev S, Ruzgas T.

Reduction of O2 at laccase modified gold nanoparticles. 4th ―NanoSchool‖. Centre of

Nanotechnology and Materials Science – NanoTechnas, Faculty of Chemistry, Vilnius

University, Lithuania, 2010-11-30

5. Krikstolaityte V, Dagys M, Kulys J, Ramanavicius A, Arnebrant T, Shleev S, Ruzgas T.

Reduction of O2 at laccase modified gold nanoparticles. The 61st Annual Meeting of the

International Society of Electrochemistry. Nice, France, 27-09-2010

6. Lindh L. "Salivary films formed on different materials – future applications", IADR,

Barcelona, 2010

7. Lindh L. Invited speaker: Oral Mouthfeel. IADR, Barcelona . J Dent Res, 89: Sp Iss .

2010







34

8. Shleev S, Falk M, Sotres J, Blum Z, Ruzgas T. Semiconductor-like behaviour of a fungal

laccase. 4th ―NanoSchool‖. Centre of Nanotechnology and Materials Science –

NanoTechnas, Faculty of Chemistry, Vilnius University, Lithuania, 2010-11-30

9. Shleev S. Implantable glucose-oxygen biofuel cell. 217th Meeting of the

Electrochemical Society. Vancouver, Canada, 27-04-2010

10. Shleev S. Semiconductor-like behaviour of a fungal laccase. The 61st Annual Meeting

of the International Society of Electrochemistry. Nice, France, 27-09-2010

11. Sotres J. AFM a versatile tool for mapping surface properties with nm resolution. 4th

―NanoSchool‖. Centre of Nanotechnology and Materials Science – NanoTechnas,

Faculty of Chemistry, Vilnius University, Lithuania, 2010-11-30

12. Wennerberg A. Invited speaker: Implant treatment. Seminar Gent University, Belgium

February 26th 2010, 8h

13. Wickström C. Invited speaker at the IADR Annual meeting, Barcelona July 17th, 2010

14. Wickström C. Mucin interaction with microorganisms. IADR, 88th general session,

Barcelona, Spain, July14-17, 2010.

National

15. Arnebrant T. Invited speaker: "Properties of salivary and mucin films on different

materials and possibilities for multicomponent surface assemblies", 24 March 2010,

Chalmers, Materials and Health Platform in the Strategic Materials Initiative at

Chalmers and GU Biomaterials arranged by Peter Thomsen, GU and Martin Andersson,

Chalmers

16. Davies J. Invited speaker at 'The Pufendorf Institute, Lund, March 29th, 2010

17. Davies J.-Invited speaker at 'The Department of Oral Biology, KI, Stockholm, March 5th,

2010

18. Engblom J. Invited speaker: ―Utan vatten – inget upptag‖ Medeon AB, 25th anniversary)

May 15th, 2010

19. Lindh L. ―Biofilm formation in oral environment – saliva and salivary films‖, Nationella

forskarskolan i Odontologi, Göteborg

20. Lindh L. Invited speaker: ―Saliv och saliversättningsmedel‖, vid Mun- &

Halscancerförbundets riksstämma, Knivsta, 2010

21. Lindh L. Invited speaker: ―Tandvård – nya tandvårdsstödet. Vad gäller för dig som

strålbehandlats‖, vid Mun- & Halscancerförbundets riksstämma, Knivsta, 2010

22. Lindh L. Invited speaker: ‖Den nya tandvårdstaxan‖, samt hjälpmedel för mun- och

halscancerdrabbade vid lokalföreningen Mun- och Halscancerföreningens rehab-möte,

Ronneby Brunn, 2010

23. Lindh L. Invited speaker: ‖Hjälpmedel för mun- och halscancerdrabbade‖, vid

lokalföreningen Mun- och Halscancerföreningens rehab-möte, Ronneby Brunn, 2010

24. Svensäter G. Invited speaker at 'Cross-disciplinary course in Biomaterials', Göteborg,

February 3rd

, 2010

25. Svensäter G. Invited speaker at 'Diplomkurs I Odontologisk Teknologi', Göteborg, May

27th, 2010

26. Svensäter G. Invited speaker at 'The Pufendorf Institute, Lund, March 29th, 2010

27. Wennerberg A. Invited speaker: Forskning och framtid för dentala implantat.

Tylösandsdagarna 15-17 September 2010.

28. Wennerberg A. Invited speaker: Surfaces: Where are we today and where are we going?

The Gothenburg Research and Technology Forum. Gothenburg, Sweden 21-22 October

2010.

29. Wennerberg A. Invited speaker: Teknologi inom tandvården. Medicin för tekniker,

Lunds universitet, 16/3 2010.







35

30. Wennerberg A. Invited speaker: Topographical measurements- techniques and

applications. 2010 03 04 1h. In research course ―Models and Methods in Dental Implant

Research. Sahlgrenska Academy, Göteborg.

Local

31. Arnebrant T, Kocherbitov V. Drug carrier interactions at biologically relevant interfaces.

Members Day of Biofilms Feb10, 2010, Malmö

32. Arnebrant T, Kocherbitov V. Interactions between pharmaceutical materials and water.


33. Arnebrant T. Adsorption and biofilm formation at oral interfaces, Members Day of

Biofilms Feb10, 2010, Malmö

34. Davies J, Investigation of interactions between osteopontin and oral biofilm bacteria,


35. Eriksson H, Arnebrant T. New concept for lipid based surface coatings in bioassays.


36. Fagerström A, Adjuvants for products used in agriculture, Members Day of Biofilms

Feb10, 2010, Malmö

37. Fagerström A, Factors affecting transport of tebuconazole over silicone and leaf cuticle.

6th Annual Workshop of Biofilms, Malmö.

38. Maimaitiyili T, The misfit strain analysis on hydrides with synchrotron radiation. 6th

Annual Workshop of Biofilms, Malmö.

39. Ng JBS, Using mesophorous silica spheres as biomolecule supports. 6th Annual

Workshop of Biofilms, Malmö.

40. Pihl M. Halvtidsseminarie Feb12, 2010., ―Biofilms on peritoneal dialysis catheters‖,

Opponent: Prof Pentti Tengvall, Sahlgrenska academy, Göteborg.

41. Pihl M. Microbial biofilms in patients with peritoneal dialysis catheters. 6th Annual

Workshop of Biofilms, Malmö.

42. Pihl M. Microbial biofilms in patients with peritoneal dialysis catheters. PhD day Oct 13

2010, , 6th Annual Workshop of Biofilms, Malmö

43. Pihl M. Microbial biofilms on peritoneal dialysis catheters. Members Day of Biofilms

Feb10, 2010, Malmö

44. Ståhle P, Biologically induced stress corrosion crack growth. Members Day of Biofilms

Feb10, 2010, Malmö

45. Ståhle P, Investigation and modeling of convection in biofilms for different carriers,


46. Svanborg L, On the importance of nanometer structures for implant incorporation in

bone soft tissue. 6th Annual Workshop of Biofilms, Malmö.

47. Svensäter G, Sjödin T. Adsorption and biofilm formation at oral interfaces, Members

Day of Biofilms Feb10, 2010, Malmö

48. Wennerberg A, Arnebrant T. Implants and their interface towards bone tissue. Members

Day of Biofilms Feb10, 2010, Malmö

49. Wennerberg A. An example of a PhD program that influenced the development of oral

implants. 6th Annual Workshop of Biofilms, Malmö.

50. Wennerberg A. Invited speaker: Development of oral implants. Examples of research

contribution. The 6th annual workshop of Biofilms. Research Center for Biointerfaces,

October 13-15th, 2010.

51. Znamenskaya Y, Effect of hydration on structural and thermodynamic properties of

mucin. 6th Annual Workshop of Biofilms, Malmö.







36

10.2 Posters 2010

International

1. Dorkhan M, Chàvez de Paz L, Davies JR. Effect of titanium surface roughness on

adhesion of Streptococcus oralis. IADR, 88th general session, Barcelona, Spain, July14-

17, 2010.

2. Kindblom C, Wickström C, Svensäter G. Binding of Streptococcus mutans to salivary

components. IADR, 88th general session, Barcelona, Spain, July14-17, 2010.

3. Kocherbitov V, Arnebrant T. Adsorption of water at solid-vapour and solid-solid

interfaces. 24th Conference of the European Colloid and Interface Society (ECIS 2010),

Prague, September 5-10, 2010

4. Neilands J, Svensäter G. Effect of fluoride and probiotics on plaque acid tolerance.

IADR, 88th general session, Barcelona, Spain, July14-17, 2010.

5. Svanborg L, Hoffman M, Andersson M, Wennerberg A. The effect of hydroxyapatite-

nanocrystals on early bone formation surrounding dental implants. IADR, Barcelona,

15th of July 2010. ID 1656

6. Svensson O and Arnebrant T. Adsorption of serum albumin on silica: Influence of

surface cleaning procedures. 24th Conference of the European Colloid and Interface

Society (ECIS 2010), Prague, September 5-10, 2010

7. Znamenskaya Y, Engblom J, Sotres J, Arnebrant T and Kocherbitov V. Effect of

hydration on structural and thermodynamic properties of mucin. 24th Conference of the

European Colloid and Interface Society (ECIS 2010), Prague, September 5-10, 2010

National

8. Björklund S, Sparr E, Engblom J, Thuresson K. The effect of water and other small polar

molecules on skin permeability, Swedish Neutron Scattering Society Meeting and

Biointerfaces, August 24-27, 2010

9. Engblom J, Pedersen L, Nilsson P, Kocherbitov V. Cubic phases in the

DOPS/DOPE/water system. MAX-lab Annual user meeting, Lund, November 8-10, 2010

10. Fagerström A., Kocherbitov V., Lamberg P., Bergström K., Westbye P., Ruzgas T.,

Engblom J., Factors affecting transport of tebuconazole over silicone membrane and leaf

cuticle, 10th ASCS – Molecular Processes at Solid Surfaces, Lund, November 24-26,

2010.

Local

11. Barauskas J, Cervin C, Jankunec M, Špandyreva M, Ribokaitė K, Tiberg F, Johnsson M.

Interactions of lipid-based liquid crystalline nanoparticles with model and cell. 6th


12. Björklund S, Dahi I, Engblom J, Sparr E, Ruzgas T. Skin response to hydration

confirmed by impedance spectroscopy. 6th Annual Workshop of Biofilms, Malmö.

13. Björklund S, Sparr E, Engblom J, Thuresson K. The effect of water and other small polar

molecules on skin permeability. 6th Annual Workshop of Biofilms, Malmö.

14. Dagys M, Shleev S, Arnebrant T, Niaura G, Kulys J, Ruzgas T. Bioelectrocatalysis of

oxygen reduction with Trametes hirsuta laccase immobilized on gold nanoparticles. 6th


15. Engblom J, Pedersen L, Nilsson P, Kocherbitov V. Cubic phases in the

DOPS/DOPE/water system. 6th Annual Workshop of Biofilms, Malmö.

16. Fagerström A., Kocherbitov V., Lamberg P., Bergström K., Westbye P., Ruzgas T.,

Engblom J. Factors affecting transport of tebuconazole over silicone membrane and leaf

cuticle. 6th Annual Workshop of Biofilms, Malmö.







37

17. Hamit-Eminovski J, Eskilsson K, Arnebrant T. An ellipsometry study on the effect of

aluminium chloride and ferric chloride formulations on mucin layers adsorbed at

hydrophobic surfaces. 6th Annual Workshop of Biofilms, Malmö.

18. Kindblom C., Wickström C., Svensäter G. Binding of Streptococcus mutans to salivary

components. 6th Annual Workshop of Biofilms, Malmö.

19. Neilands J, Petersson L, Beighton D, Svensäter G. Fluoride inhibits acid tolerance of root

surface biofilms. 6th Annual Workshop of Biofilms, Malmö.

20. Pedersen L, Wahlgren M, Engblom J. Effect of a gradient in water chemical potential on

buccal drug delivery. 6th Annual Workshop of Biofilms, Malmö.

21. Znamenskaya Y, Engblom J, Sotres J, Arnebrant T and Kocherbitov V. Effect of

hydration on structural and thermodynamic properties of mucin. 6th Annual Workshop of

Biofilms-Research Center for Biointerfaces, Malmö, October 13-15, 2010.

11 BSc and MSc education

11.1 BSc-level

1. Biomedical laboratory science (HS)

2. TELMah-Biomedical Technology (TS & HS)

3. Dentistry program (OD)

11.2 MSc-level

1. Biomedical Methods and Technology (HS)

2. Materials science (TS & HS)

3. Dentistry program (OD)

12 Other Activities by Center Members Gunnel Svensäter and Tautgirdas Ruzgas have been opponents at dissertations for doctors

degree at Faculty of Health Sciences, Copenhagen University, and at Dept of Physics,

Linköping University, respectively. Gunilla Nordin Fredrikson has been opponent at a

half-time control (Faculty of Health Sciences, Linköping University). Håkan Eriksson,

Bertil Kinnby, Julia Davies, Anette Gjörloff-Wingren, Ann Wennerberg, Liselott Lindh,

Tautgirdas Ruzgas, Thomas Arnebrant and Per Såhle have been board members at

dissertations at 12 occasions (Lnu, LU, GU, LuTH, Mah) and Julia Davies and Gunnel

Svensäter have been external examiners at Leeds Dental Institute, UK. Julia Davies has

also been external examiner at Trinity College Dublin, Ireland. Gunilla Nordin

Fredrikson, Liselott Lindh, Per Ståhle, Tautgirdas Ruzgas and Thomas Arnebrant have

been external reviewers for promotions to professor (2), associate professor (1) and

employment as senior lecturer (3).

Center members have been referees for international journals on a regular basis. The list

of journals include; Acta Biomaterialia, Acta Oncologica, Analytical Chemistry,

Angewandte Chemie International Edition, Arteriosclerosis, Thrombosis and Vascular,

Biology (ATVB), Atherosclerosis, Biofouling, Biomacromolecules, Biosensors and







38

Bioelectronics, Biotechnology Progress, Bioelectrochemistry, Caries research, Chemistry

Communications, Clinical Immunology, Colloid and Polymer Science, Colloids and

Surfaces B: Biointerfaces, Colloids and Surfaces A, Current Opinion in Colloid and

Interface Science, Electroanalysis, Electrochimica Acta, Electrochemical

Communications, European Heart Journal, European Journal of Oral Sciences, European

Journal of Histochemistry, Future Cardiology, Industrial & Engineering Chemistry

Research, International Journal of Biomedical Science (IJBS), Journal of Clinical

Pathology, Journal of Proteome Research , Journal of the American Chemical Society

(JACS), Journal of Biomedical Materials Research: Part A, Journal of Internal Medicine,

Journal of Molecular Signaling, Journal of Physical Chemistry B, Journal of Chemical

Physics, Journal of Colloid and Interface Science, Journal of Biomedical Biochemistry

(JBB), Journal of Food Engineering, Langmuir, Macromolecules, Scandinavian Journal

of Immunology, Sensors and actuators, Talanta, Water Environment Research, JJOM,

IJSS, JAM, European Journal of Dental Education, Proteome Science, Journal of

Periodontal Research, Thrombosis and Haemostasis, PLoS, Cancer Letters, International

Journal of Endodontics, Caries Research, Journal of Medical Microbiology,

Microbiology, Archives Oral Biology, Archives of Oral Biology, Journal of Oral

Rehabilitation, Journal of Biomedical Materials Research, Biofouling, Journal of Colloid

and Interface Science, Microbiology, Journal of Colloids and Surfaces B: Biointerfaces,

Biomacromolecules, Journal of Clinical Periodontology, Acta Biomaterialia, Oral

Diseases, European Journal of Oral Sciences, Journal of Dental research.

Ann Wennerberg is on the editorial board for Acta Biomaterialia, International Journal of

Prosthodontics and Journal of Oral Rehabilitation. Tautgirdas Ruzgas is on the editorial

board for Nonlinear analysis: Modelling and control, and Per Ståhle was guest editor of

International Journal of Fracture.

Ann Wennerberg was coordinator, and Gunnel Svensäter was the local coordinator at

Mah, of the Swedish National Graduate School in Odontological Science. Svensäter was

also member of the scientific advisory board at the Swedish Council on Technology

Assessment in Health Care (SBU) and the Research committee at Faculty of Odontology

(OD, Mah). Julia Davies was Director of Postgraduate Research Education, delegate on

Faculty Working Group for Quality Development, delegate on Faculty Library Working

Group and faculty representative on University group for Research Education. Ann

Wennerberg was member of the scientific advisory board at the European Association for

Osseointegration and board member at Swedish research counsil (NT-A). Anette Gjörloff

Wingren was chairman of the Education board at Faculty of Health and society.

Tautgirdas Ruzgas was member of the docent board at Malmö University. Johan

Engblom was member of the Quality assurance group and the Education board at Faculty

of Health and society, and member of the Faculty Board at School of Technology (TS,

Mah). Gunilla Nordin Fredrikson was convener of the Evaluation committee of DPLU

(Diabetes Program Lund University) and consulted as a judgement expert of Biomedical

Scientist educations in Sweden by the National Agency of Higher Education. Thomas

Arnebrant was at the faculty board of Health and society, and also assistant Dean.

Cover story

Biofilms annual workshop this year attracted 110 guests from different universities as

well as industries, innovation agencies, solicitor’s offices and risk capitalists. During the

three day workshop a wide range of interesting lectures were held on the topic

―Biomaterials - From Fundamentals to Market Application‖.

- We are very happy that we this year could combine the annual workshop with the

workshop of an EU-project as it allowed us to offer lectures on research results as well as

on the commercialization of scientific innovations, says Johan Engblom, Director of

Biofilms - Research Center for Biointerfaces

- It has been a very interesting workshop! I have got an up date on what is going on, and

at the same time I have had the opportunity to meet many interesting people in the area,

says Christy Whiddon, AkzoNobel Surface Chemistry AB.

[Malmö University News Letter, 2010-12-09]

.

Center Mission Statement

Biofilms – Research Center for Biointerfaces is a translational research programme

covering six research groups within three faculties/schools (HS, OD & TS) at Malmö

University. The core strengths of the Center is i) our broad expertise, spanning the range

from theoretical modeling to clinical sciences, and ii) our long experience in working

with industry and relating to their needs.

The general aim of the research activities at the Center is to understand, predict and

control material/cell/tissue interactions with medical, dental, food and environmental

applications. We strive to further integrate education (BSc, MSC, PhD), a cornerstone for

the future of the Center. Particularly, one goal is to further integrate Master-level

education into our research activities also at an operational level in specific projects.

Biofilms – Research Center for Biointerfaces has a goal to become a regional venue for a

creative environment of biomedical technology in the Öresund region, facilitating

collaboration between academic research, higher education and industry, based on

pharmaceutical technology, biotechnology and medical technology, which together

constitutes a key area of significant commercial growth potential. We strive to become a

prime entry point for regional industry seeking translational academic expertise in the

Biomedical technology field.

Center Location

Biofilms – Research Center for Biointerfaces

Malmö University

SE-205 06 MALMÖ, Sweden

Center director: Assoc. Prof. Johan Engblom (JE)

Tel: +46-(0)706-08 75 25 (JE); +46-(0)40-66 57 486 (Adm. Coordinator Eva Nilsson)

e-mail: [email protected]

www.mah.se/biofilms

Visiting adress:

Skåne University Hospital, SUS (Entrance 49)

MALMÖ

http://www.mah.se/biofilms

biofilms research center for biointerfaces · from the center activities, now established as two of...

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