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Page 1: Aquabayproducts

Aqua ByAqua By--Products 1Products 1--04 AL04 ALJoe McElweeJoe McElwee

IFA AquacultureIFA Aquaculture

Page 2: Aquabayproducts

Aqua ByAqua By--Products 1Products 1--04 AL04 ALJoe McElweeJoe McElweeIFA AquacultureIFA Aquaculture

Partners

Irish Farmers’ Association (IFA) Aquaculture – Lead

CIMA – Centro de Investigacions Marinas

Norsk Sjombedrifters LandsforeningNSL

Page 3: Aquabayproducts

Aqua ByAqua By--Products 1Products 1--04 AL04 ALJoe McElweeJoe McElweeIFA AquacultureIFA Aquaculture

ObjectivesTo compile a databank of information on waste management suppliers, facilities and consultants to provide an electronic ”one-stop shop” for aquaculture and processing companies. to find appropriate outlets for by-products of their business,within national and EU regulations. The project aims to benefit both sectors by increasing awareness, communication and networking possibilities through the Internet over an economically and geographically diverse sector.

Objective and targets: To optimise the range and volume of by-products from the aquaculture industry that are re-used or recycled by:

■ Increasing awareness among the industry of the potential outlets for its by-products

■ Increase awareness in the by-product processing sector of raw materials available

■ To encourage the development, improvement and dissemination of techniques and technology to re-use and recycle by products

■ To develop a trans-national and inter-regional network of raw material and solution

suppliers via a central database which is updated regularly

Page 4: Aquabayproducts

www.aquabyproducts.com

!! ““With the waste industry worth With the waste industry worth €€1.2 1.2 Billion, coupled with new laws for recycling Billion, coupled with new laws for recycling and product development, we should and product development, we should seriously examine all possible avenues of seriously examine all possible avenues of waste disposal and recycling/regeneration, waste disposal and recycling/regeneration, including both Organic and Inorganic including both Organic and Inorganic waste items.waste items.””Waste MeetingWaste Meeting--Sunday Business Post Sunday Business Post --Jan 2006Jan 2006

Page 5: Aquabayproducts

www.aquabyproducts.com

!! ““We have moved from a hump and dump We have moved from a hump and dump industry to huge investment in new industry to huge investment in new products, markets and technology. There is products, markets and technology. There is an increasingly sophisticated and technical an increasingly sophisticated and technical approach to waste management, with a approach to waste management, with a focus on compliance and regulationfocus on compliance and regulation””

Irish Waste Industry View Conference 2006Irish Waste Industry View Conference 2006

Page 6: Aquabayproducts

Has the website been successful !!! Over 5,000 Hits Over 5,000 Hits !! From all around the worldFrom all around the world!! Huge European InterestHuge European Interest!! New Technology & New Technology &

information becoming information becoming availableavailable

!! New Grant Aid Schemes New Grant Aid Schemes from Irish Governmentfrom Irish Government

!! Definite Irish Industry Definite Irish Industry InterestInterest

!! Quantification of Waste Quantification of Waste necessary necessary

!! Irish companies proposal Irish companies proposal for business with Partnersfor business with Partners

!! Government/Agency Government/Agency support and visionsupport and vision

!! Logistic’s & Management Logistic’s & Management of wasteof waste

Page 7: Aquabayproducts

www.aquabyproducts.comThe way forward!!! Need to continue the successful web siteNeed to continue the successful web site!! Assess and quantify Irish Production of WasteAssess and quantify Irish Production of Waste!! This will require further This will require further significant fundingsignificant funding for for

Administration and Operation of websiteAdministration and Operation of website!! Examine further “Partnership” projects/business with Examine further “Partnership” projects/business with

industry Waste Companies in Irelandindustry Waste Companies in Ireland!! Look at the future European developments in both Look at the future European developments in both

regulatory and monetary implications with regards to wasteregulatory and monetary implications with regards to waste!! Establish links with new industry/products that utilise our Establish links with new industry/products that utilise our

waste, waste, ie ie Cosmetics & PlasticsCosmetics & Plastics

Page 8: Aquabayproducts

www.aquabyproducts.com

!! Assess a project with significant business potential Assess a project with significant business potential between the three partnersbetween the three partners

!! Entice & encourage Waste company participation Entice & encourage Waste company participation between the three partner countriesbetween the three partner countries

!! Use the geographical diversity to develop the Use the geographical diversity to develop the aquaculture waste aquaculture waste byproduct byproduct industryindustry

!! We have achieved exposure, divested much We have achieved exposure, divested much knowledge, established many serious business knowledge, established many serious business contacts…..so lets take it to the next step!contacts…..so lets take it to the next step!

Page 9: Aquabayproducts

Business on a real Scale

Page 10: Aquabayproducts

FORUM AquaReg May 2006FORUM FORUM AquaRegAquaReg May 2006May 2006

MODIFIED ATMOSPHERE PACKAGING FOR COLD CHAIN TRANSPORT OF FRESH MUSSEL OVER SHORT AND LONG

DISTANCES

MODIFIED ATMOSPHERE PACKAGING FOR COLD CHAIN MODIFIED ATMOSPHERE PACKAGING FOR COLD CHAIN TRANSPORT OF FRESH MUSSEL TRANSPORT OF FRESH MUSSEL OVEROVER SHORT AND LONG SHORT AND LONG

DISTANCEDISTANCESS(Project ID: 2-05 AL, AquaGair)

ParticipantsCentro Tecnológico del Mar- Fundación CETMAR (Galicia)

Marine Research Institute IIM-CSIC (Galicia)

Letterkenny Institute of Technology LYIT (Ireland)

Centro Centro TecnológicoTecnológico del Mardel Mar-- FundaciónFundación CETMAR CETMAR (Galicia)(Galicia)

Marine Research Institute IIMMarine Research Institute IIM--CSIC (Galicia)CSIC (Galicia)

LetterkennyLetterkenny Institute of Technology LYIT (Ireland)Institute of Technology LYIT (Ireland)

Collaboration: OPMEGA (Galician Mussels Producers Association)CollaborationCollaboration: : OPMEGA (Galician Mussels Producers Association)OPMEGA (Galician Mussels Producers Association)

Page 11: Aquabayproducts

1. Project Aims

• Use of Modified Atmosphere Packaging (MAP) technologies to extend the shelf life of fresh mussels in hermetic packaging.

•Design and optimization of in-pack gas atmosphere in order to obtain maximum survival of packed mussels.

–active atmosphere modification, high barrier films, to achieve maximum benefits in a short time.

–comparison of viability of MAP mussels to air packed mussels

–the effect of different in-pack oxygen concentrations on mussels survival and determination of optimum in-pack gas concentrations

–the effect of temperature on mussel survival

•Transfer of optimised MAP system to other geographical varieties and to study the effect of MAP in Galician mussel (Mytillus galloprovincialis) and Irish mussel (Mytillus edulis), defining optimum survival conditions for both species.

•Determine the effect of different MAP conditions on Quality Indices

• Verify mussel survival rates and quality through the cold chain.

Page 12: Aquabayproducts

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Storage Time (Days)

% M

orta

lity 65%

75%85%Air

Mortality rates of bottom mussels stored at 7-8°C

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% M

orta

lity 65%

75%85%Air Sample

Mortality rates of bottom mussels stored at 2-3°C

2. Results: Effect of O2 concentration and temperature

Page 13: Aquabayproducts

0123456

2 5 7 9

Storage Time (days)

% M

ort

alit

y65%

75%

85%

Air

Mortality Rates of rope mussels stored at 2-3°C

2. Results: The effect of O2 concentration on rope mussels

Page 14: Aquabayproducts

2. Results: The Effect of Cold Transport

Irish mussels

Galician mussels

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% mortality after cold transport (storage 2°C)% % mortalitymortality afterafter cold cold transporttransport ((storagestorage 22°C°C))

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2. Results: The Effect of TemperatureTransport

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% m

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Control w ithout transport 6ºC

Transport 3,5 ºC

Transport 10ºC

1ºC

Galician mussels

Page 16: Aquabayproducts

2. Results: Quality

Organoleptic Characteristics:Mussels stored in high oxygen and low temperature performed better than mussels stored in air and higher temperatures.

Microbiological Quality:

Tests: VRBA at 44°C and 32°C, PCA at 4°C and 35°C

In general microbiological quality seems to improve with MAP, however results are inconclusive

Page 17: Aquabayproducts

3. Conclusions:

•Mussels stored at lower temperatures have longer shelf lives.

•Mussels stored under high oxygen concentrations have a longer shelf life than those stored in air, as reflected by better sensory ratings and lower mortality rates.

•Mussels stored at 85% oxygen have a longer shelf life than those stored at lower oxygen concentrations, as reflected by better sensory ratings and lower mortality rates.

•Rope mussels react at least as well to high oxygen MAP as bottomfarmed mussels.

•Mussels stored under MAP conditions are suitable for cold-chain transport

•Microbial analysis of high oxygen MAP mussels is inconclusive.

Page 18: Aquabayproducts

BIOPURALG: reducing the environmental impact of land based aquaculture through

cultivation of seaweeds

Stefan Kraan

Irish Seaweed Centre, Martin Ryan Institute, National University of Ireland, Galway, Ireland

SINTEF, Fisheries and Aquaculture, NO-7465 TRONDHEIM, Norway

Oyster Creek Seafoods Limited, Maree, Oranmore, County Galway, Ireland

Page 19: Aquabayproducts

Introduction

• Discharge of nutrients from land based finfish and shellfish culitivation systems.

• Major concern everywhere. • Marine algae can act as bio-filters and remove

pollutants producing a clean waste water effluent.

• The principle is to direct effluent water from rearing ponds into tanks in which unattached seaweeds are maintained in permanent motion using aeration.

• This allows for the production of value added seaweed biomass

• Sources for cosmetics, pharmaceutical, fine chemicals, biomedicines, food and animal feed

Page 20: Aquabayproducts

• Protein is the principal source of nitrogenous pollution in fish and shellfish aquaculture, either from excess feed or excretions and reaches the water, mainly as dissolved ammonia

• The removal of this pollution is possible using marine algae as bio-filters.

• The SME partners in this project is concerned of the levels in his discharge water from the oyster holding units.

• Introduction of a seaweed bio-filter can resolve his problem while producing another value added product, i.e., seaweed biomass.

Page 21: Aquabayproducts

Main objectives

• Design and set up seaweed biofilter system for land based fin-fish hatchery and for Oyster and mussel holding unit

• Optimum species evaluation • Testing the ability of the chosen species to strip N and P from

(discharge) waters• Measuring protein levels in cultivated species and comparisons

with similar species from pristine areas• Measuring biomass of the cultivated seaweeds• Screen cultivated seaweed species for bioactivity against fish

and human pathogens• Explore applications of the harvestable algal biomass for the

development of food, animal feed and pharmaceuticals

Page 22: Aquabayproducts

What species?• Choice of species• 13 of 29 recent investigations have used Ulva as the

experimental seaweed in integrated systems. The only commercial scale seaweed-based integrated fish & abalone farm in operation uses Ulva as a biofilters to remove nutrients from the water and also as feed for the Abalone. However, other algae may be better candidates, both as biofilters and as a value-added secondary product.

• Experience with Palmaria and Asparagopsis• The red alga Porphyra was chosen for the first trials in

this project.

Page 23: Aquabayproducts

Pros and cons

Pros• High production and nutrient accumulation• Valuable pigments• Excellent Abalone and Urchin feed • Different species available at different times

Cons• Seasonality• Temperature sensitivity• Loss of tissue with onset of reproduction

Page 24: Aquabayproducts

Methods• Seaweed Biofilter system• A cascading tank system (3m3 – 2m3 –

1m3) was supplied with UV-treated effluent water from the fin-fish hatchery at MRI Carna

• Seaweed tanks were stocked with field collected Porphyra at a density of ~0.5 kg m-³ (1.5, 1 and 0.5 kg resp.)

• Thalli were kept in motion by vigorous aeration. Biomass was removed from the tanks weekly and the wet weight measured. Tanks were restocked at the original stocking density after measurement.

• Water temperature and light intensity were continuously monitored using in-situ dataloggers.

.

Page 25: Aquabayproducts

• A second larger system established at Oyster Creek New design of Bio-filter tanks: Two tank-systems installed

• Total capacity of biofilter 24 m3

• Header tank 120 m3

• Stocking densities 5,1.5 and 1 kg

Page 26: Aquabayproducts

Nutrient measurements

• Ammonia, Nitrate, Nitrite and Phosphate

• Chemical reaction and measured spectrophotometrically

• Protein (Kjeldahl)

Page 27: Aquabayproducts

Antibacterial activity

• Antibacterial activity of biofilter species• Bacterial inhibition was evaluated as growth inhibition of specific

test bacteria by the agar diffusion method.• Two bacteria isolated from diseased cod juveniles; Listonella

anguillarum (HI610) and Marinomonas sp. Were used. • Sterile 6 mm paper discs were treated with 2 x 25 µl of seaweed-

extract or solvent (negative control) and allowed to dry betweeneach addition.

• Discs were thereafter applied to agar plates seeded with 100 µl of 24 h culture of test bacteria and incubated (20°C). Inhibition zones (radius from discs in mm) were registered after 24 hours.

Page 28: Aquabayproducts

Results

• Initial results have shown that Porphyra can be used successfully as a biofilter for removing excess nutrient from Irish land-based aquaculture operations.

• A significant reduction in ammonia, phosphate and nitrate has been achieved in the initial trial.

• Yields of up to 34 gFW m-2 day-1 were achieved but this decreased as the temperature rose.

• Therefore over summer months Ulva as biofilter

Page 29: Aquabayproducts
Page 30: Aquabayproducts

Nutrient reduction in mg/l

0

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0.25

0.3

0.35

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0.45

NH4 in NH4 out NO2 in NO2 out NO3 in NO3 out PO4 PO4

Page 31: Aquabayproducts

Average Monthly Yield Ulva (gFW m-2 day-1)

R2 = 0.3373

0.00

5.00

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25.00

july aug sept oct nov

Page 32: Aquabayproducts

Tank 1 Tank 2 Tank 3

Page 33: Aquabayproducts

0

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NH4 in NH4 out NO2 in NO2 out NO3 in NO3 out PO4 PO4

Nutrient reduction in mg/l

Page 34: Aquabayproducts

Average nutrient removal using Porphyra and Ulva

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NH4 NO2 NO3 PO4

UlvaPorphyra

Page 35: Aquabayproducts

Average nutrient removal using Porphyra and Ulva per unit yield of algae

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NH4 NO2 NO3 PO4

UlvaPorphyra

Page 36: Aquabayproducts

Bioactivity of crude extracts

Page 37: Aquabayproducts

Initial Protein results

• Protein levels increased slightly with an average of 5-10%

• Ulva around 30% of dry weight compared to 20-25% in species from pristine sites

• Porphyra around 35% compared to 25-30% in wild species

• More samples to be analysed

Page 38: Aquabayproducts

Other achieved outputs

• New linkages and enterprise contacts: Contact made with other land-based aquaculture operations interested in the technology (e.g. Abalone producers)

• Planning for related proposal with current and other international partners

• New design of Bio-filter tanks: Two tank-systems installed and being tested

• New products : ! Bio-active compounds, Bioactive properties identified in

crude extracts! High protein abalone and urchin feed

Page 39: Aquabayproducts

Major problems encountered

• Planning permission– 1.6 year delay for Oyster Creek

• DHL– Samples for Sintef

(frozen seaweeds) stuck in

customs. Happened twice

(10 days and 31 days) with

winter and spring samples

Page 40: Aquabayproducts

Conclusions

• Porphyra dioica is a suitable species for use as a biofilter under Irish conditions

• A consistent harvest of excess material was obtained, although the yield dropped as temperatures increased. This fall off in biomass production would suggest that this species is not the most suitable during the summer

• Trials using the green alga, Ulva lactuca, has proven to be suitable over the summer and autumn period

• Ulva proved to be a good nutrient scrubber and a consistent harvest of excess material was obtained. The potential benefits of usingother species is also being considered

• Cultivated seaweed biomass can be used for other products• Ammonia and nitrate can be stripped from the water using the dual

species system at rates varying from 60% up to 90% of the nutrient input and Phosphate at 40% removal

Page 41: Aquabayproducts

Conclusions

• Antibiotic activity against fish and human pathogens has been observed in both Ulva, Palmaria and Porphyraextracts.

• The results show that seaweed biofilters can be used in land based aquaculture systems to clean waste water streams while providing a value added product.

• Under Irish conditions it is suggested to use a biofiltersystem with Porphyra from October to April and Ulva from May to September for the highest rate of nutrient removal from land-based aquaculture waste water streams.

Page 42: Aquabayproducts

Challenges

• Proving feasibility at commercial scale

• Ensuring economic viability of the technology

• To do this we need the opportunity to test the large system at Oyster Creek (extension?)

Page 43: Aquabayproducts

Thank you for your attention

Page 44: Aquabayproducts

CARRASEAApproach to a sustainable exploitation of carrageen

seaweed resources in Galicia and Ireland

Assesing the state of populations of the carrageenan producing red algae in Galicia and Ireland comparing the population dynamics of exploited and unexploited populations

•LPP D.X .Recursos Mariños (Galicia)•PP CETMAR (Galicia), USC (Galicia), Irish Seaweed Centre (MRI-NUIG) (BMW)

AQUALINK

Page 45: Aquabayproducts

Background

Galicia:

!Carrageenan extraction industry

!Decline in harvest (1200 T in 70s to 225 T in 2005)

BMW:

!Health food markets and seavegetable sector

!Decline in harvest (300 T in 60s to 40 T in 2005)

Similarities

!Carrageen industry

!Poor knowledge about resource

!Resources are under exploited

!Harvesting unchanged

!Decline in harvest!Demand higher than supply.

!Sustainability??

Main goal:

Development of resource management knowledge to support thesustainable exploitation of carrageen moss.

Page 46: Aquabayproducts

Main activities:!Assessment of the current state of the carrageen harvesting sector in Ireland (Galway-BMW) and Galicia.

!Assessment of the impact of harvesting activity on the population of seaweeds of interest (recovery and productivity).

!Study of the dynamics of the carrageenophyte populations.

!Quantitative and qualitative analysis of carrageen production.

!Study of the genetic variability.

Page 47: Aquabayproducts

Assessment of the current state of the carrageen harvesting sector1) Carragen harvesting laws: BMW: Foreshore Act.

Galicia: Ley Pesca; Decreto 423.

2) Harvesting licence requirements: BMW: Seaweed harvesting- Foreshore licence (DoC,M&NR).Galicia: “Explotation plain” (CPAM).

3) Profile of Harvesters: BMW: Mostly people over 40.Galicia: Mostly people over 40.

4) Main activity of harvesters: BMW: farmers or inshore fishermen.Galicia: shellfishers or farmers.

5) Harvesters number: BMW: 100?Galicia: 500?

6) T/year harvested: BMW: 40 T/year DW (2005).Galicia: 225 T/year DW (2005).

7) Main markets: BMW: Sea-vegetable / healthfood market, cosmetic.Galicia: Carrageen extraction

8) Price/kg: BMW: 2.5 to 3.2 €/kg (DW). Galicia: 0.75 €/kg (DW).

9) Level of organization: BMW: Individual and occasional activity,co-op formation encouraged.

Galicia: Fishermen association and harvesters ` commissioned by processing industry

Page 48: Aquabayproducts

Threats

! Lack of harvesters.

! Lack of interest in younger generations.

! Lack of business diversity.

! Low price.

! Cheap imports and competition.

! Pollution.

! Impact of invasive species.

Page 49: Aquabayproducts

TARGET SPECIES

Common name: Irish/carrageen moss, “liquen”

Chondrus crispus Mastocarpus stellatus

Page 50: Aquabayproducts

Life cycle of Chondrus crispus

Mastocarpus stellatus

Only gametophyte phase is exploited. Tetrasporophyte is a microscopic encrusting thallus.

Life cycle of Chondrus from Chen and Mc Lachlan (1972)

Male Gametophyte

FemaleGametophyte

Carposoporphytes

Tetrasporophyte

TetrasporesCarpospores

Exploited phases:

Gametophytes and Tetrasporophytes.

Page 51: Aquabayproducts

SAMPLING AREAS

4 sites around Galway bay with different wave exposure. For each site, 2 spots, upper and lower shore.

3 sites: on the North (Cantabric Sea), Middle (Costa da Morte) and South ofGalicia.

Page 52: Aquabayproducts

Chondrus crispus Laxe

December 2004 before harvesting December 2004 after harvesting March 2005

June 2005 September 2005 January 2006

Total denudation

Cut

Chondrus crispus Laxe

December 2004 before harvesting December 2004 after harvesting March 2005

June 2005 September 2005 January 2006

Total denudation

Cut

Assessment of the impact of harvesting activity on the population of seaweeds of interest (recovery and productivity).

Page 53: Aquabayproducts

Time series of Finavarra Denude regrowth Jan 05-Jan 06

January 2005 March 2005 May 2005

September 2005 November 2005 January 2006

Page 54: Aquabayproducts

Successional studies related to exploitation techniques based on the percentage of the plot area covered by carrageenan seaweeds

Chondrus crispus Mastocarpus stellatus

MOUGÁS

LAXE

SAN ROMÁN

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Page 55: Aquabayproducts

Assessment of the impact of harvesting activity on the population of seaweeds of interest (recovery and

productivity): Galicia and BMW.

! After one year, the areas where the algae were hand picked or cut, recovered their initial status for both species (C. crispus and M. Stellatus), reaching values close to those of the control areas.

! In the case of total denudation, the rate of recovery was lower, although differences related to the locations were observed.

Page 56: Aquabayproducts

Dynamics of the carragenophyte population analysis (size classes)

Page 57: Aquabayproducts

Sizing of Chondrus samples

Page 58: Aquabayproducts

Chondrus crispus recruitment(% number of fronds < 5 cm)

Mougás Laxe San Román

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Page 59: Aquabayproducts

Seasonal changes of gametophytes and tetrasporophytesin Chondrus crispus populations (% N)

Laxe (Galicia)

Braizon (BMW)

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Page 60: Aquabayproducts

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San Román (Galicia)

Page 61: Aquabayproducts

Seasonal changes in fructified fronds of Chondrus crispus (% N )

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San RománLaxeMougás

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San RománLaxeMougás

TetrasporophyteGametophyte

Page 62: Aquabayproducts

Dynamics of the carrageenophyte populations

!Recruitment of juvenile fronds was observed during the year for both species in the two regions.

!Temporal and spatial variation in the recruitment rate were observed.

!The growth rate of the C. crispus fronds was more or less uniform along the year for the three Galician sites. In BMW, some differences were recorded between sheltered and exposed sites.

! Differences in the proportion of gametophytic and tetrasporophytic fronds of C. crispus were observed among locations. Also, there were seasonal variations among and between populations.

!The growth rate of the M. stellatus fronds reached the maximum values in summer for the three Galician sites.

!The maximum percentages of fructified fronds were recorded in autumn and winter in both regions.

Page 63: Aquabayproducts

Quantitative and qualitative carrageenan analysis.

Extraction Homogenizationand filtration

ConcentrationPrecipitation

Filtration

Coagulation

Crudeextracts

Freeze dried

Page 64: Aquabayproducts

Quantitative analysis of carrageenan production

Chondrus crispus gametophytesCrude carrageenan content

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%

San RománLaxeMougás

Chondrus crispus tetrasporophytesCrude carrageenan content

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San RománLaxeMougás

M astocarpus stellatus gametophytesCrude carrageenan content

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%

San RománLaxeMougás

Galicia

Page 65: Aquabayproducts

M astocarpus stellatus gametophytesCrude carrageenan content

34,238,1

36,834,9

47,79

33,5830,81

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Mougás

Laxe

San Román

Blackh

ead

Finnavarr

a

SpiddalBrai

zon

Galicia BMW% DW

Page 66: Aquabayproducts

Qualitative carrageenan analysis

1H NMR spectra of commercial carrageenan(Sigma – Aldrich)

ι-carrageenan

λ-carrageenan

κ-carrageenan

Chemical shifts referred to acetone 2.225 ppm

Page 67: Aquabayproducts

Qualitative carrageenan analysis

Seasonal changes in carrageenan composition of Chondrus crispus gametophytes

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12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05

Laxe Mougás San Román

%

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Qualitative carrageenan analysis

Seasonal changes in carrageenan composition of Mastocarpus stellatus gametophytes

0

10

20

30

40

50

60

70

80

12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05

Laxe Mougás San Román

%

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Qualitative analysis of carrageenan production.

FT-IR carrageenan spectra

Ch. c. TEF

Ch.c. GF

M. s. GF

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Quantitative and qualitative carrageenan analysis

!The crude carrageenan extracts for both species fluctuated between 20 and 50% of the dry weight in the two regions.

!The highest values of carrageenans for C. crispus and M stellatusgametophytes were observed in San Román – Galicia (values higher than 60% were observed).

!Regarding the tetrasporophytes, differences among locations were not observed.

!There were seasonal differences in carrageenans quantities:

•Maximum values in summer and minimum values in early spring in Galicia (C. crispus and M. stellatus).

•Maximum values in late autumn and the minimum in summer in BMW (M. stellatus).

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M 1 2 3 4 5 6 7 8 9 10 B M 1 2 3 4 5 6 7 8 9 10 B M 1 2 3 4 5 6 7 8 B

Chondrus crispusSan Román, Galicia, gametophytes

Mastocarpus stellatusLaxe, Galicia, gametophytes

Mastocarpus stellatusSpiddal, Co. Galway, gametophytes

M: molecular weight marker

RAPD patterns obtained from individuals (lanes 1-10) and bulked genomic DNA samples (lane B), with primer OPA-03 for Chondrus crispus and with primer OPA-10 for Mastocarpus stellatus

RAPD comparison bettween individual and bulked methods

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Chondrus crispus gametophytes

Comparison among RAPD patterns obtained from bulked samples containinggenomic DNA from 10 individuals in four Galician populations

OPA-02

MWM SR L M PSP

OPA-03

MWM SR L M PSP

OPA-13

MWM SR L M PSP

OPA-19

MWM SR L M PSP

3000 bp

2000 bp

1000 bp

250 bp

3000 bp

1000 bp

250 bp

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Mastocarpus stellatus gametophytes

Comparison among RAPD patterns obtained from bulked samples containing genomic DNAfrom 10 individuals in Galician populations and 8 individuals in Irish populations

OPA-02

SR L M B F S BR MWM

OPA-03

SR L M B F S BR MWM

OPA-04

SR L M B F S BR MWM

OPA-20

SR L M B F S BR MWM

OPA-13

MWM SR L M B F S BR

OPA-18

MWM SR L M B F S BR

MWM: molecular weight markerGalician populations- SR: San Román; L: Laxe; M: MougásIrish populations- B:Blackhead; F:Finavarra; S:Spiddal; BR:Brezon

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Genetic variability

The results till now obtained show that:

! Strong genetic differences ocurred within GalicianChondrus crispus populations

! Strong genetic differences ocurred within andbetween Galician and Irish Mastocarpus stellatuspopulations

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www.carrasea.org

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RecommendationsFuture collaboration, exchange of information, and experience

between Galicia and BMW to foster development of LOW VOLUME-HIGH VALUE SECTORS.

• Skills development / training.

• Marketing development and consumer awareness

• Results with potential use to provide scientific basis forsustainable harvesting and resource management.

• Expansion of the sampling period is advised to clarify seasonalvariation of population dynamic and carrageenan content.

• R&D of aquaculture of carrageenophytes

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Interreg Community Initiative Programmes

AquaReg Forum, Galway, May 23rd 2006

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Presentation overview

! Interreg Programmes in Ireland

! Features & Challenges of Interreg 2000-2006

! Future of Interreg post 2006

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Interreg Programmes 2000-2006

1. Interreg IIIA Cross Border Ireland/Northern IrelandIreland/Wales

2. Interreg IIIB Transnational Atlantic Area North West Europe

3. Interreg IIIC Interregional

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Cross Border regions of Europe

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Ireland/Wales IIIA Programme Ireland/N.Ireland IIIA Programme

INTERREG IIIA CROSS BORDER PROGRAMMES IN IRELAND

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Interreg IIIB - Transnational

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Interreg IIIC -Interregional

North: Rostock, Germany

East: Vienna, Austria

South: Valencia, Spain

West: Lille, France

North

East

South

West

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Interreg – Common themes

! Polycentric development/Spatial Devlpt. ! Accessibility & Transport infrastructure ! ICT & the Knowledge Economy ! The Environment, Maritime & Natural Resources! R & D, Innovation, Technology Transfer ! Culture, Tourism & heritage ! Business Linkages! Local development strategies

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Main Beneficiaries

! Universities & Research Centres! Local & Regional Authorities! Leader Groups ! Semi state bodies ! Community & Voluntary bodies ! BICs ! Private sector

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What Interreg is worth to Ireland –some examples

Interreg IIIA Ireland/Wales €28m

Interreg IIIB Atlantic Area €15m

Interreg IIIB North West Europe €17m

Interreg IIIC (All Zones) €7.4m

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The BMW Regional Assembly & Interreg

! National contact point for Interreg IIIB Atlantic Area Programme

! Member of PSC and PMC of Atlantic Area! PMC member of Interreg IIIA

Ireland/Northern Ireland Programme ! PSC & PMC member of Interreg IIIC

(West Zone) programme

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Benefits of INTERREG Co-operation! The overall aim of the INTERREG initiatives has

been, and remains, that national borders should not be a barrier to the balanced development and integration of European territory.

! Although the single market and EMU have been strong catalysts for change, the scope for strengthening co-operation to the mutual advantage of border areas throughout the Community is considerable.

! The challenge is all the greater when the enlargement of the Community is considered, as this has increased the number of the EU’s internal borders and has shifted the Community’s external borders eastwards & Southwards

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Benefits of INTERREG – contd.! INTERREG is seen to be making a significant

contribution towards balanced development and is helping social and economic cohesion.

! INTERREG is seen to be making a significant contribution to domestic policies. It is also seen to be a demonstration of the benefits of sharing experiences and best practices.

! Studies across the Community show respondents to be keen about the added value from innovative projects and joint investment.

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Overall

INTERREG’s cross-border, transnational, and inter-regional programmes are perceived to be delivering added value with potential for more.

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Challenges for the future

! Greater use of INTERAct by partners & other EU technical support networks

! Greater State involvement in projects ! Financial Management of projects ! Greater Industry/Academia collaboration ! Greater private sector involvement ! More research by organisations into

potential partnerships ! Projects led from Ireland ! Greater sustainability of projects

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Structural Funds Post 2006

! In Dec 2005 in Brussels EU Leaders reached agreement on an EU Budget of 1.045% of EU GNI (€862 billion)

! EU Leaders agreed a structural funds budget of €308 billion for the 2007 – 2013 period.

! Three priorities which will be funded through the ERDF and the ESF.

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Three Cohesion Policy Objectives

! Objective 1 - Convergence

! Objective 2 - Regional Competitiveness and Employment

! Objective 3 - Territorial Co-operation

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Objective One : Convergence

! This concerns Member States and regions whose per capita GDP is less than 75% of the Community average.

! The key objective is to promote growth-enhancing conditions and factors leading to real convergence.

! Funding agreed of 81.9% or € 252 billion of Structural Funds.

! No Irish regions will be eligible

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Objective Two : Regional Competitiveness and employment

! This will focus on the more developed Member States and regions and will:

! a. consist of development programmes designed tohelp regions to anticipate and promote economic change by strengthening their competitiveness and attractiveness; and

! b. interventions aimed at creating more and better jobs by adapting the workforce to economic change.

! Funding agreed of 15.7% or € 48.4 billion. Ireland’s indicative allocation is €811m (which covers objectives 2 & 3)

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Objective ThreeEuropean Territorial Co-operation.

! This objective is intended to support co-operation between regions at cross-border, transnational, and inter-regional level in orderto further develop the harmonious and balanced integration of the Union’s territory.

! Funding agreed of €7.5bn

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Objective 3The ‘new’ INTERREG

! INTERREG is to be an Objective in its own right.

! It will have slightly increased funding as theagreed allocation between 2007 - 2013 is €7.5bn(2.4% ).

! This compares to 2.5% or €5.8 billion of Structural Funds in the current period.

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Objective 3 - continued

! Co-financing of 50% likely in most programmes

! Decentralisation : there will be a stronger role for the regions and local players

! Legal Basis : there will be a new regulation –the ‘European Grouping of Cross-Border Co-operation’ ( EGCC)

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Objective 3 – Eligibility & Priorities

! Eligibility will not be based on MS levels of GDP

! The whole of the European Union territory will be eligible for financing of European co-operation and exchange networks

! Priorities will be:- Innovation, R & D, Technology Transfer- Environment / risk prevention/ Maritime safety/coastal mgt - Accessibility, ICT, Transport systems- Culture, education

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Objective 3 – Three Elements

! Cross-border co-operation

! Transnational co-operation

! Programmes for networks and exchange of experience

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Objective 3 – Financial Allocations (current)

! Cross Border €5.8bn

! Transnational €1.4bn

! Interregional €300m

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CROSS BORDER area 2007-2013

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Irish participation in Interreg2007-2013

! Continuation of Ireland/Wales cross border programme

! Possible extension of Ireland/N. Ireland cross border programme to include western Scotland

! Atlantic Area Transnational continuation ! NWE transnational continuation! Potential involvement in new Northern Periphery

programme ! Interreg Interregional programme continuation

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Contact Details

BMW Regional Assembly, The Square, Ballaghaderreen, Co. Roscommon. Phone: 094-9862970Fax: 094-9862973 e-mail: [email protected]