aquabayproducts
TRANSCRIPT
Aqua ByAqua By--Products 1Products 1--04 AL04 ALJoe McElweeJoe McElwee
IFA AquacultureIFA Aquaculture
Aqua ByAqua By--Products 1Products 1--04 AL04 ALJoe McElweeJoe McElweeIFA AquacultureIFA Aquaculture
Partners
Irish Farmers’ Association (IFA) Aquaculture – Lead
CIMA – Centro de Investigacions Marinas
Norsk Sjombedrifters LandsforeningNSL
Aqua ByAqua By--Products 1Products 1--04 AL04 ALJoe McElweeJoe McElweeIFA AquacultureIFA Aquaculture
ObjectivesTo compile a databank of information on waste management suppliers, facilities and consultants to provide an electronic ”one-stop shop” for aquaculture and processing companies. to find appropriate outlets for by-products of their business,within national and EU regulations. The project aims to benefit both sectors by increasing awareness, communication and networking possibilities through the Internet over an economically and geographically diverse sector.
Objective and targets: To optimise the range and volume of by-products from the aquaculture industry that are re-used or recycled by:
■ Increasing awareness among the industry of the potential outlets for its by-products
■ Increase awareness in the by-product processing sector of raw materials available
■ To encourage the development, improvement and dissemination of techniques and technology to re-use and recycle by products
■ To develop a trans-national and inter-regional network of raw material and solution
suppliers via a central database which is updated regularly
www.aquabyproducts.com
!! ““With the waste industry worth With the waste industry worth €€1.2 1.2 Billion, coupled with new laws for recycling Billion, coupled with new laws for recycling and product development, we should and product development, we should seriously examine all possible avenues of seriously examine all possible avenues of waste disposal and recycling/regeneration, waste disposal and recycling/regeneration, including both Organic and Inorganic including both Organic and Inorganic waste items.waste items.””Waste MeetingWaste Meeting--Sunday Business Post Sunday Business Post --Jan 2006Jan 2006
www.aquabyproducts.com
!! ““We have moved from a hump and dump We have moved from a hump and dump industry to huge investment in new industry to huge investment in new products, markets and technology. There is products, markets and technology. There is an increasingly sophisticated and technical an increasingly sophisticated and technical approach to waste management, with a approach to waste management, with a focus on compliance and regulationfocus on compliance and regulation””
Irish Waste Industry View Conference 2006Irish Waste Industry View Conference 2006
Has the website been successful !!! Over 5,000 Hits Over 5,000 Hits !! From all around the worldFrom all around the world!! Huge European InterestHuge European Interest!! New Technology & New Technology &
information becoming information becoming availableavailable
!! New Grant Aid Schemes New Grant Aid Schemes from Irish Governmentfrom Irish Government
!! Definite Irish Industry Definite Irish Industry InterestInterest
!! Quantification of Waste Quantification of Waste necessary necessary
!! Irish companies proposal Irish companies proposal for business with Partnersfor business with Partners
!! Government/Agency Government/Agency support and visionsupport and vision
!! Logistic’s & Management Logistic’s & Management of wasteof waste
www.aquabyproducts.comThe way forward!!! Need to continue the successful web siteNeed to continue the successful web site!! Assess and quantify Irish Production of WasteAssess and quantify Irish Production of Waste!! This will require further This will require further significant fundingsignificant funding for for
Administration and Operation of websiteAdministration and Operation of website!! Examine further “Partnership” projects/business with Examine further “Partnership” projects/business with
industry Waste Companies in Irelandindustry Waste Companies in Ireland!! Look at the future European developments in both Look at the future European developments in both
regulatory and monetary implications with regards to wasteregulatory and monetary implications with regards to waste!! Establish links with new industry/products that utilise our Establish links with new industry/products that utilise our
waste, waste, ie ie Cosmetics & PlasticsCosmetics & Plastics
www.aquabyproducts.com
!! Assess a project with significant business potential Assess a project with significant business potential between the three partnersbetween the three partners
!! Entice & encourage Waste company participation Entice & encourage Waste company participation between the three partner countriesbetween the three partner countries
!! Use the geographical diversity to develop the Use the geographical diversity to develop the aquaculture waste aquaculture waste byproduct byproduct industryindustry
!! We have achieved exposure, divested much We have achieved exposure, divested much knowledge, established many serious business knowledge, established many serious business contacts…..so lets take it to the next step!contacts…..so lets take it to the next step!
Business on a real Scale
FORUM AquaReg May 2006FORUM FORUM AquaRegAquaReg May 2006May 2006
MODIFIED ATMOSPHERE PACKAGING FOR COLD CHAIN TRANSPORT OF FRESH MUSSEL OVER SHORT AND LONG
DISTANCES
MODIFIED ATMOSPHERE PACKAGING FOR COLD CHAIN MODIFIED ATMOSPHERE PACKAGING FOR COLD CHAIN TRANSPORT OF FRESH MUSSEL TRANSPORT OF FRESH MUSSEL OVEROVER SHORT AND LONG SHORT AND LONG
DISTANCEDISTANCESS(Project ID: 2-05 AL, AquaGair)
ParticipantsCentro Tecnológico del Mar- Fundación CETMAR (Galicia)
Marine Research Institute IIM-CSIC (Galicia)
Letterkenny Institute of Technology LYIT (Ireland)
Centro Centro TecnológicoTecnológico del Mardel Mar-- FundaciónFundación CETMAR CETMAR (Galicia)(Galicia)
Marine Research Institute IIMMarine Research Institute IIM--CSIC (Galicia)CSIC (Galicia)
LetterkennyLetterkenny Institute of Technology LYIT (Ireland)Institute of Technology LYIT (Ireland)
Collaboration: OPMEGA (Galician Mussels Producers Association)CollaborationCollaboration: : OPMEGA (Galician Mussels Producers Association)OPMEGA (Galician Mussels Producers Association)
1. Project Aims
• Use of Modified Atmosphere Packaging (MAP) technologies to extend the shelf life of fresh mussels in hermetic packaging.
•Design and optimization of in-pack gas atmosphere in order to obtain maximum survival of packed mussels.
–active atmosphere modification, high barrier films, to achieve maximum benefits in a short time.
–comparison of viability of MAP mussels to air packed mussels
–the effect of different in-pack oxygen concentrations on mussels survival and determination of optimum in-pack gas concentrations
–the effect of temperature on mussel survival
•Transfer of optimised MAP system to other geographical varieties and to study the effect of MAP in Galician mussel (Mytillus galloprovincialis) and Irish mussel (Mytillus edulis), defining optimum survival conditions for both species.
•Determine the effect of different MAP conditions on Quality Indices
• Verify mussel survival rates and quality through the cold chain.
0
10
20
30
40
50
60
1 3 6 8 10
Storage Time (Days)
% M
orta
lity 65%
75%85%Air
Mortality rates of bottom mussels stored at 7-8°C
0
1
2
3
4
1 3 6 8 10Storage Time (Days)
% M
orta
lity 65%
75%85%Air Sample
Mortality rates of bottom mussels stored at 2-3°C
2. Results: Effect of O2 concentration and temperature
0123456
2 5 7 9
Storage Time (days)
% M
ort
alit
y65%
75%
85%
Air
Mortality Rates of rope mussels stored at 2-3°C
2. Results: The effect of O2 concentration on rope mussels
2. Results: The Effect of Cold Transport
Irish mussels
Galician mussels
0
5
10
15
20
25
30
35
40
1 2 3 4 5 6 7 8 9 10Days
% m
orta
lity
Air
MAP
Air transport
MAP transport
% mortality after cold transport (storage 2°C)% % mortalitymortality afterafter cold cold transporttransport ((storagestorage 22°C°C))
0
5
10
15
20
25
30
35
40
1 2 3 4 5 6 7 8 9 10Days
% m
orta
lity
Air
MAP
Air transport
MAP transport
2. Results: The Effect of TemperatureTransport
0
5
10
15
20
25
30
35
1 2 3 4 5 6 7 8Days
% m
orta
lity
Control w ithout transport 6ºC
Transport 3,5 ºC
Transport 10ºC
1ºC
Galician mussels
2. Results: Quality
Organoleptic Characteristics:Mussels stored in high oxygen and low temperature performed better than mussels stored in air and higher temperatures.
Microbiological Quality:
Tests: VRBA at 44°C and 32°C, PCA at 4°C and 35°C
In general microbiological quality seems to improve with MAP, however results are inconclusive
3. Conclusions:
•Mussels stored at lower temperatures have longer shelf lives.
•Mussels stored under high oxygen concentrations have a longer shelf life than those stored in air, as reflected by better sensory ratings and lower mortality rates.
•Mussels stored at 85% oxygen have a longer shelf life than those stored at lower oxygen concentrations, as reflected by better sensory ratings and lower mortality rates.
•Rope mussels react at least as well to high oxygen MAP as bottomfarmed mussels.
•Mussels stored under MAP conditions are suitable for cold-chain transport
•Microbial analysis of high oxygen MAP mussels is inconclusive.
BIOPURALG: reducing the environmental impact of land based aquaculture through
cultivation of seaweeds
Stefan Kraan
Irish Seaweed Centre, Martin Ryan Institute, National University of Ireland, Galway, Ireland
SINTEF, Fisheries and Aquaculture, NO-7465 TRONDHEIM, Norway
Oyster Creek Seafoods Limited, Maree, Oranmore, County Galway, Ireland
Introduction
• Discharge of nutrients from land based finfish and shellfish culitivation systems.
• Major concern everywhere. • Marine algae can act as bio-filters and remove
pollutants producing a clean waste water effluent.
• The principle is to direct effluent water from rearing ponds into tanks in which unattached seaweeds are maintained in permanent motion using aeration.
• This allows for the production of value added seaweed biomass
• Sources for cosmetics, pharmaceutical, fine chemicals, biomedicines, food and animal feed
• Protein is the principal source of nitrogenous pollution in fish and shellfish aquaculture, either from excess feed or excretions and reaches the water, mainly as dissolved ammonia
• The removal of this pollution is possible using marine algae as bio-filters.
• The SME partners in this project is concerned of the levels in his discharge water from the oyster holding units.
• Introduction of a seaweed bio-filter can resolve his problem while producing another value added product, i.e., seaweed biomass.
Main objectives
• Design and set up seaweed biofilter system for land based fin-fish hatchery and for Oyster and mussel holding unit
• Optimum species evaluation • Testing the ability of the chosen species to strip N and P from
(discharge) waters• Measuring protein levels in cultivated species and comparisons
with similar species from pristine areas• Measuring biomass of the cultivated seaweeds• Screen cultivated seaweed species for bioactivity against fish
and human pathogens• Explore applications of the harvestable algal biomass for the
development of food, animal feed and pharmaceuticals
What species?• Choice of species• 13 of 29 recent investigations have used Ulva as the
experimental seaweed in integrated systems. The only commercial scale seaweed-based integrated fish & abalone farm in operation uses Ulva as a biofilters to remove nutrients from the water and also as feed for the Abalone. However, other algae may be better candidates, both as biofilters and as a value-added secondary product.
• Experience with Palmaria and Asparagopsis• The red alga Porphyra was chosen for the first trials in
this project.
Pros and cons
Pros• High production and nutrient accumulation• Valuable pigments• Excellent Abalone and Urchin feed • Different species available at different times
Cons• Seasonality• Temperature sensitivity• Loss of tissue with onset of reproduction
Methods• Seaweed Biofilter system• A cascading tank system (3m3 – 2m3 –
1m3) was supplied with UV-treated effluent water from the fin-fish hatchery at MRI Carna
• Seaweed tanks were stocked with field collected Porphyra at a density of ~0.5 kg m-³ (1.5, 1 and 0.5 kg resp.)
• Thalli were kept in motion by vigorous aeration. Biomass was removed from the tanks weekly and the wet weight measured. Tanks were restocked at the original stocking density after measurement.
• Water temperature and light intensity were continuously monitored using in-situ dataloggers.
.
• A second larger system established at Oyster Creek New design of Bio-filter tanks: Two tank-systems installed
• Total capacity of biofilter 24 m3
• Header tank 120 m3
• Stocking densities 5,1.5 and 1 kg
Nutrient measurements
• Ammonia, Nitrate, Nitrite and Phosphate
• Chemical reaction and measured spectrophotometrically
• Protein (Kjeldahl)
Antibacterial activity
• Antibacterial activity of biofilter species• Bacterial inhibition was evaluated as growth inhibition of specific
test bacteria by the agar diffusion method.• Two bacteria isolated from diseased cod juveniles; Listonella
anguillarum (HI610) and Marinomonas sp. Were used. • Sterile 6 mm paper discs were treated with 2 x 25 µl of seaweed-
extract or solvent (negative control) and allowed to dry betweeneach addition.
• Discs were thereafter applied to agar plates seeded with 100 µl of 24 h culture of test bacteria and incubated (20°C). Inhibition zones (radius from discs in mm) were registered after 24 hours.
Results
• Initial results have shown that Porphyra can be used successfully as a biofilter for removing excess nutrient from Irish land-based aquaculture operations.
• A significant reduction in ammonia, phosphate and nitrate has been achieved in the initial trial.
• Yields of up to 34 gFW m-2 day-1 were achieved but this decreased as the temperature rose.
• Therefore over summer months Ulva as biofilter
Nutrient reduction in mg/l
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
NH4 in NH4 out NO2 in NO2 out NO3 in NO3 out PO4 PO4
Average Monthly Yield Ulva (gFW m-2 day-1)
R2 = 0.3373
0.00
5.00
10.00
15.00
20.00
25.00
july aug sept oct nov
Tank 1 Tank 2 Tank 3
0
0.1
0.2
0.3
0.4
0.5
0.6
NH4 in NH4 out NO2 in NO2 out NO3 in NO3 out PO4 PO4
Nutrient reduction in mg/l
Average nutrient removal using Porphyra and Ulva
0
20
40
60
80
100
NH4 NO2 NO3 PO4
UlvaPorphyra
Average nutrient removal using Porphyra and Ulva per unit yield of algae
0
1
2
3
4
5
6
7
NH4 NO2 NO3 PO4
UlvaPorphyra
Bioactivity of crude extracts
Initial Protein results
• Protein levels increased slightly with an average of 5-10%
• Ulva around 30% of dry weight compared to 20-25% in species from pristine sites
• Porphyra around 35% compared to 25-30% in wild species
• More samples to be analysed
Other achieved outputs
• New linkages and enterprise contacts: Contact made with other land-based aquaculture operations interested in the technology (e.g. Abalone producers)
• Planning for related proposal with current and other international partners
• New design of Bio-filter tanks: Two tank-systems installed and being tested
• New products : ! Bio-active compounds, Bioactive properties identified in
crude extracts! High protein abalone and urchin feed
Major problems encountered
• Planning permission– 1.6 year delay for Oyster Creek
• DHL– Samples for Sintef
(frozen seaweeds) stuck in
customs. Happened twice
(10 days and 31 days) with
winter and spring samples
Conclusions
• Porphyra dioica is a suitable species for use as a biofilter under Irish conditions
• A consistent harvest of excess material was obtained, although the yield dropped as temperatures increased. This fall off in biomass production would suggest that this species is not the most suitable during the summer
• Trials using the green alga, Ulva lactuca, has proven to be suitable over the summer and autumn period
• Ulva proved to be a good nutrient scrubber and a consistent harvest of excess material was obtained. The potential benefits of usingother species is also being considered
• Cultivated seaweed biomass can be used for other products• Ammonia and nitrate can be stripped from the water using the dual
species system at rates varying from 60% up to 90% of the nutrient input and Phosphate at 40% removal
Conclusions
• Antibiotic activity against fish and human pathogens has been observed in both Ulva, Palmaria and Porphyraextracts.
• The results show that seaweed biofilters can be used in land based aquaculture systems to clean waste water streams while providing a value added product.
• Under Irish conditions it is suggested to use a biofiltersystem with Porphyra from October to April and Ulva from May to September for the highest rate of nutrient removal from land-based aquaculture waste water streams.
Challenges
• Proving feasibility at commercial scale
• Ensuring economic viability of the technology
• To do this we need the opportunity to test the large system at Oyster Creek (extension?)
Thank you for your attention
CARRASEAApproach to a sustainable exploitation of carrageen
seaweed resources in Galicia and Ireland
Assesing the state of populations of the carrageenan producing red algae in Galicia and Ireland comparing the population dynamics of exploited and unexploited populations
•LPP D.X .Recursos Mariños (Galicia)•PP CETMAR (Galicia), USC (Galicia), Irish Seaweed Centre (MRI-NUIG) (BMW)
AQUALINK
Background
Galicia:
!Carrageenan extraction industry
!Decline in harvest (1200 T in 70s to 225 T in 2005)
BMW:
!Health food markets and seavegetable sector
!Decline in harvest (300 T in 60s to 40 T in 2005)
Similarities
!Carrageen industry
!Poor knowledge about resource
!Resources are under exploited
!Harvesting unchanged
!Decline in harvest!Demand higher than supply.
!Sustainability??
Main goal:
Development of resource management knowledge to support thesustainable exploitation of carrageen moss.
Main activities:!Assessment of the current state of the carrageen harvesting sector in Ireland (Galway-BMW) and Galicia.
!Assessment of the impact of harvesting activity on the population of seaweeds of interest (recovery and productivity).
!Study of the dynamics of the carrageenophyte populations.
!Quantitative and qualitative analysis of carrageen production.
!Study of the genetic variability.
Assessment of the current state of the carrageen harvesting sector1) Carragen harvesting laws: BMW: Foreshore Act.
Galicia: Ley Pesca; Decreto 423.
2) Harvesting licence requirements: BMW: Seaweed harvesting- Foreshore licence (DoC,M&NR).Galicia: “Explotation plain” (CPAM).
3) Profile of Harvesters: BMW: Mostly people over 40.Galicia: Mostly people over 40.
4) Main activity of harvesters: BMW: farmers or inshore fishermen.Galicia: shellfishers or farmers.
5) Harvesters number: BMW: 100?Galicia: 500?
6) T/year harvested: BMW: 40 T/year DW (2005).Galicia: 225 T/year DW (2005).
7) Main markets: BMW: Sea-vegetable / healthfood market, cosmetic.Galicia: Carrageen extraction
8) Price/kg: BMW: 2.5 to 3.2 €/kg (DW). Galicia: 0.75 €/kg (DW).
9) Level of organization: BMW: Individual and occasional activity,co-op formation encouraged.
Galicia: Fishermen association and harvesters ` commissioned by processing industry
Threats
! Lack of harvesters.
! Lack of interest in younger generations.
! Lack of business diversity.
! Low price.
! Cheap imports and competition.
! Pollution.
! Impact of invasive species.
TARGET SPECIES
Common name: Irish/carrageen moss, “liquen”
Chondrus crispus Mastocarpus stellatus
Life cycle of Chondrus crispus
Mastocarpus stellatus
Only gametophyte phase is exploited. Tetrasporophyte is a microscopic encrusting thallus.
Life cycle of Chondrus from Chen and Mc Lachlan (1972)
Male Gametophyte
FemaleGametophyte
Carposoporphytes
Tetrasporophyte
TetrasporesCarpospores
Exploited phases:
Gametophytes and Tetrasporophytes.
SAMPLING AREAS
4 sites around Galway bay with different wave exposure. For each site, 2 spots, upper and lower shore.
3 sites: on the North (Cantabric Sea), Middle (Costa da Morte) and South ofGalicia.
Chondrus crispus Laxe
December 2004 before harvesting December 2004 after harvesting March 2005
June 2005 September 2005 January 2006
Total denudation
Cut
Chondrus crispus Laxe
December 2004 before harvesting December 2004 after harvesting March 2005
June 2005 September 2005 January 2006
Total denudation
Cut
Assessment of the impact of harvesting activity on the population of seaweeds of interest (recovery and productivity).
Time series of Finavarra Denude regrowth Jan 05-Jan 06
January 2005 March 2005 May 2005
September 2005 November 2005 January 2006
Successional studies related to exploitation techniques based on the percentage of the plot area covered by carrageenan seaweeds
Chondrus crispus Mastocarpus stellatus
MOUGÁS
LAXE
SAN ROMÁN
0
20
40
60
80
100
N04 D J05 F M A M J J A S O N04 J06
%
0
20
40
60
80
100
N04 D J05 F M A M J J A S O N04 J06
%
0
20
40
60
80
100
N04 D J05 F M A M J J A S O N04 J06
%
0
20
40
60
80
100
N04 D J05 F M A M J J A S O N04 J06
%
0
20
40
60
80
100
N04 D J05 F M A M J J A S O N04 J06
%
0
20
40
60
80
100
N04 D J05 F M A M J J A S O N04 J06
%
ControlPick upCutDenudation
Assessment of the impact of harvesting activity on the population of seaweeds of interest (recovery and
productivity): Galicia and BMW.
! After one year, the areas where the algae were hand picked or cut, recovered their initial status for both species (C. crispus and M. Stellatus), reaching values close to those of the control areas.
! In the case of total denudation, the rate of recovery was lower, although differences related to the locations were observed.
Dynamics of the carragenophyte population analysis (size classes)
Sizing of Chondrus samples
Chondrus crispus recruitment(% number of fronds < 5 cm)
Mougás Laxe San Román
0
10
20
30
40
50
60
70
80
90
100
Dec_04 Feb_05 Apr J un Aug Oc t J an_060
10
20
30
40
50
60
70
80
90
100
Dec_04 Feb_05 Apr J un Aug Oct J an_060
10
20
30
40
50
60
70
80
90
100
Dec_04 Feb_05 Apr J un Aug Oct J an_06
< 5 cm
> 5 cm
Seasonal changes of gametophytes and tetrasporophytesin Chondrus crispus populations (% N)
Laxe (Galicia)
Braizon (BMW)
0
10
20
30
40
50
60
70
80
90
Jan-05
Feb-05Mar-0
5Apr-0
5
May-05
Jun-05Jul-0
5
Aug-05
Sep-05Oct-0
5
Nov-05
Dec-05Jan-06
% a
vera
ge fo
rsite
GametophyteTetrasporophyte
0
20
40
60
80
100
Dec Feb_05 Apr Jun Aug Oct Jan_06
%
0
20
40
60
80
100
Dec Feb_05 Apr Jun Aug Oct Jan_06
%
Black Head (BMW)
0
10
20
30
40
50
60
70
80
90
Jan-05Feb-05
Mar-05
Apr-05
May-05
Jun-05Jul-0
5
Aug-05
Sep-05Oct-0
5Nov-05
Dec-05Jan-06
% a
vera
ge fo
rsite
GametophyteTetrasporophyte
San Román (Galicia)
Seasonal changes in fructified fronds of Chondrus crispus (% N )
0
5
10
15
20
25
Dec_04 Feb_05 Apr Jun Aug Oct Jan_06
%
San RománLaxeMougás
0
5
10
15
20
25
Dec_04 Feb_05 Apr Jun Aug Oct Jan_06
%
San RománLaxeMougás
TetrasporophyteGametophyte
Dynamics of the carrageenophyte populations
!Recruitment of juvenile fronds was observed during the year for both species in the two regions.
!Temporal and spatial variation in the recruitment rate were observed.
!The growth rate of the C. crispus fronds was more or less uniform along the year for the three Galician sites. In BMW, some differences were recorded between sheltered and exposed sites.
! Differences in the proportion of gametophytic and tetrasporophytic fronds of C. crispus were observed among locations. Also, there were seasonal variations among and between populations.
!The growth rate of the M. stellatus fronds reached the maximum values in summer for the three Galician sites.
!The maximum percentages of fructified fronds were recorded in autumn and winter in both regions.
Quantitative and qualitative carrageenan analysis.
Extraction Homogenizationand filtration
ConcentrationPrecipitation
Filtration
Coagulation
Crudeextracts
Freeze dried
Quantitative analysis of carrageenan production
Chondrus crispus gametophytesCrude carrageenan content
0
10
20
30
40
50
60
70
DEC_0 4 FEB_0 5 APR_ 0 5 JUN_ 05 AUG_ 05 OCT_0 5 JAN_ 06
%
San RománLaxeMougás
Chondrus crispus tetrasporophytesCrude carrageenan content
0
10
20
30
40
50
60
70
DEC_0 4 FEB_ 05 APR_ 05 JUN_0 5 AUG_0 5 OCT_ 05 JAN_ 06
%
San RománLaxeMougás
M astocarpus stellatus gametophytesCrude carrageenan content
0
10
20
30
40
50
60
70
DEC_0 4 FEB_0 5 APR_ 05 JUN_0 5 AUG_0 5 OCT_0 5 JAN_ 06
%
San RománLaxeMougás
Galicia
M astocarpus stellatus gametophytesCrude carrageenan content
34,238,1
36,834,9
47,79
33,5830,81
0,00
10,00
20,00
30,00
40,00
50,00
60,00
Mougás
Laxe
San Román
Blackh
ead
Finnavarr
a
SpiddalBrai
zon
Galicia BMW% DW
Qualitative carrageenan analysis
1H NMR spectra of commercial carrageenan(Sigma – Aldrich)
ι-carrageenan
λ-carrageenan
κ-carrageenan
Chemical shifts referred to acetone 2.225 ppm
Qualitative carrageenan analysis
Seasonal changes in carrageenan composition of Chondrus crispus gametophytes
0
10
20
30
40
50
60
70
80
12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05
Laxe Mougás San Román
%
ννννιιιιµµµµκκκκ
Qualitative carrageenan analysis
Seasonal changes in carrageenan composition of Mastocarpus stellatus gametophytes
0
10
20
30
40
50
60
70
80
12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05 12-04 03-05 06-05 09-05
Laxe Mougás San Román
%
ννννιιιιµµµµκκκκ
Qualitative analysis of carrageenan production.
FT-IR carrageenan spectra
Ch. c. TEF
Ch.c. GF
M. s. GF
Quantitative and qualitative carrageenan analysis
!The crude carrageenan extracts for both species fluctuated between 20 and 50% of the dry weight in the two regions.
!The highest values of carrageenans for C. crispus and M stellatusgametophytes were observed in San Román – Galicia (values higher than 60% were observed).
!Regarding the tetrasporophytes, differences among locations were not observed.
!There were seasonal differences in carrageenans quantities:
•Maximum values in summer and minimum values in early spring in Galicia (C. crispus and M. stellatus).
•Maximum values in late autumn and the minimum in summer in BMW (M. stellatus).
M 1 2 3 4 5 6 7 8 9 10 B M 1 2 3 4 5 6 7 8 9 10 B M 1 2 3 4 5 6 7 8 B
Chondrus crispusSan Román, Galicia, gametophytes
Mastocarpus stellatusLaxe, Galicia, gametophytes
Mastocarpus stellatusSpiddal, Co. Galway, gametophytes
M: molecular weight marker
RAPD patterns obtained from individuals (lanes 1-10) and bulked genomic DNA samples (lane B), with primer OPA-03 for Chondrus crispus and with primer OPA-10 for Mastocarpus stellatus
RAPD comparison bettween individual and bulked methods
Chondrus crispus gametophytes
Comparison among RAPD patterns obtained from bulked samples containinggenomic DNA from 10 individuals in four Galician populations
OPA-02
MWM SR L M PSP
OPA-03
MWM SR L M PSP
OPA-13
MWM SR L M PSP
OPA-19
MWM SR L M PSP
3000 bp
2000 bp
1000 bp
250 bp
3000 bp
1000 bp
250 bp
Mastocarpus stellatus gametophytes
Comparison among RAPD patterns obtained from bulked samples containing genomic DNAfrom 10 individuals in Galician populations and 8 individuals in Irish populations
OPA-02
SR L M B F S BR MWM
OPA-03
SR L M B F S BR MWM
OPA-04
SR L M B F S BR MWM
OPA-20
SR L M B F S BR MWM
OPA-13
MWM SR L M B F S BR
OPA-18
MWM SR L M B F S BR
MWM: molecular weight markerGalician populations- SR: San Román; L: Laxe; M: MougásIrish populations- B:Blackhead; F:Finavarra; S:Spiddal; BR:Brezon
Genetic variability
The results till now obtained show that:
! Strong genetic differences ocurred within GalicianChondrus crispus populations
! Strong genetic differences ocurred within andbetween Galician and Irish Mastocarpus stellatuspopulations
www.carrasea.org
RecommendationsFuture collaboration, exchange of information, and experience
between Galicia and BMW to foster development of LOW VOLUME-HIGH VALUE SECTORS.
• Skills development / training.
• Marketing development and consumer awareness
• Results with potential use to provide scientific basis forsustainable harvesting and resource management.
• Expansion of the sampling period is advised to clarify seasonalvariation of population dynamic and carrageenan content.
• R&D of aquaculture of carrageenophytes
Interreg Community Initiative Programmes
AquaReg Forum, Galway, May 23rd 2006
Presentation overview
! Interreg Programmes in Ireland
! Features & Challenges of Interreg 2000-2006
! Future of Interreg post 2006
Interreg Programmes 2000-2006
1. Interreg IIIA Cross Border Ireland/Northern IrelandIreland/Wales
2. Interreg IIIB Transnational Atlantic Area North West Europe
3. Interreg IIIC Interregional
Cross Border regions of Europe
Ireland/Wales IIIA Programme Ireland/N.Ireland IIIA Programme
INTERREG IIIA CROSS BORDER PROGRAMMES IN IRELAND
Interreg IIIB - Transnational
Interreg IIIC -Interregional
North: Rostock, Germany
East: Vienna, Austria
South: Valencia, Spain
West: Lille, France
North
East
South
West
Interreg – Common themes
! Polycentric development/Spatial Devlpt. ! Accessibility & Transport infrastructure ! ICT & the Knowledge Economy ! The Environment, Maritime & Natural Resources! R & D, Innovation, Technology Transfer ! Culture, Tourism & heritage ! Business Linkages! Local development strategies
Main Beneficiaries
! Universities & Research Centres! Local & Regional Authorities! Leader Groups ! Semi state bodies ! Community & Voluntary bodies ! BICs ! Private sector
What Interreg is worth to Ireland –some examples
Interreg IIIA Ireland/Wales €28m
Interreg IIIB Atlantic Area €15m
Interreg IIIB North West Europe €17m
Interreg IIIC (All Zones) €7.4m
The BMW Regional Assembly & Interreg
! National contact point for Interreg IIIB Atlantic Area Programme
! Member of PSC and PMC of Atlantic Area! PMC member of Interreg IIIA
Ireland/Northern Ireland Programme ! PSC & PMC member of Interreg IIIC
(West Zone) programme
Benefits of INTERREG Co-operation! The overall aim of the INTERREG initiatives has
been, and remains, that national borders should not be a barrier to the balanced development and integration of European territory.
! Although the single market and EMU have been strong catalysts for change, the scope for strengthening co-operation to the mutual advantage of border areas throughout the Community is considerable.
! The challenge is all the greater when the enlargement of the Community is considered, as this has increased the number of the EU’s internal borders and has shifted the Community’s external borders eastwards & Southwards
Benefits of INTERREG – contd.! INTERREG is seen to be making a significant
contribution towards balanced development and is helping social and economic cohesion.
! INTERREG is seen to be making a significant contribution to domestic policies. It is also seen to be a demonstration of the benefits of sharing experiences and best practices.
! Studies across the Community show respondents to be keen about the added value from innovative projects and joint investment.
Overall
INTERREG’s cross-border, transnational, and inter-regional programmes are perceived to be delivering added value with potential for more.
Challenges for the future
! Greater use of INTERAct by partners & other EU technical support networks
! Greater State involvement in projects ! Financial Management of projects ! Greater Industry/Academia collaboration ! Greater private sector involvement ! More research by organisations into
potential partnerships ! Projects led from Ireland ! Greater sustainability of projects
Structural Funds Post 2006
! In Dec 2005 in Brussels EU Leaders reached agreement on an EU Budget of 1.045% of EU GNI (€862 billion)
! EU Leaders agreed a structural funds budget of €308 billion for the 2007 – 2013 period.
! Three priorities which will be funded through the ERDF and the ESF.
Three Cohesion Policy Objectives
! Objective 1 - Convergence
! Objective 2 - Regional Competitiveness and Employment
! Objective 3 - Territorial Co-operation
Objective One : Convergence
! This concerns Member States and regions whose per capita GDP is less than 75% of the Community average.
! The key objective is to promote growth-enhancing conditions and factors leading to real convergence.
! Funding agreed of 81.9% or € 252 billion of Structural Funds.
! No Irish regions will be eligible
Objective Two : Regional Competitiveness and employment
! This will focus on the more developed Member States and regions and will:
! a. consist of development programmes designed tohelp regions to anticipate and promote economic change by strengthening their competitiveness and attractiveness; and
! b. interventions aimed at creating more and better jobs by adapting the workforce to economic change.
! Funding agreed of 15.7% or € 48.4 billion. Ireland’s indicative allocation is €811m (which covers objectives 2 & 3)
Objective ThreeEuropean Territorial Co-operation.
! This objective is intended to support co-operation between regions at cross-border, transnational, and inter-regional level in orderto further develop the harmonious and balanced integration of the Union’s territory.
! Funding agreed of €7.5bn
Objective 3The ‘new’ INTERREG
! INTERREG is to be an Objective in its own right.
! It will have slightly increased funding as theagreed allocation between 2007 - 2013 is €7.5bn(2.4% ).
! This compares to 2.5% or €5.8 billion of Structural Funds in the current period.
Objective 3 - continued
! Co-financing of 50% likely in most programmes
! Decentralisation : there will be a stronger role for the regions and local players
! Legal Basis : there will be a new regulation –the ‘European Grouping of Cross-Border Co-operation’ ( EGCC)
Objective 3 – Eligibility & Priorities
! Eligibility will not be based on MS levels of GDP
! The whole of the European Union territory will be eligible for financing of European co-operation and exchange networks
! Priorities will be:- Innovation, R & D, Technology Transfer- Environment / risk prevention/ Maritime safety/coastal mgt - Accessibility, ICT, Transport systems- Culture, education
Objective 3 – Three Elements
! Cross-border co-operation
! Transnational co-operation
! Programmes for networks and exchange of experience
Objective 3 – Financial Allocations (current)
! Cross Border €5.8bn
! Transnational €1.4bn
! Interregional €300m
CROSS BORDER area 2007-2013
Irish participation in Interreg2007-2013
! Continuation of Ireland/Wales cross border programme
! Possible extension of Ireland/N. Ireland cross border programme to include western Scotland
! Atlantic Area Transnational continuation ! NWE transnational continuation! Potential involvement in new Northern Periphery
programme ! Interreg Interregional programme continuation
Contact Details
BMW Regional Assembly, The Square, Ballaghaderreen, Co. Roscommon. Phone: 094-9862970Fax: 094-9862973 e-mail: [email protected]