advia 120 troubleshooting guide

PRELIMINARY PRODUCT INFORMATIONControl Number: 024-0005-14Revision: B

PPI #024-0005-14 Hydraulic Troubleshooting Guide March 1999 Rev B

ADVIA 120 Hydraulic Troubleshooting GuideDate of Issue: 3/24/99

Subject: Hydraulic Troubleshooting Guide

Products: ADVIA 120

ADVIA 120

HYDRAULIC TROUBLESHOOTING GUIDE

The following is a troubleshooting chart that attempts to guide Field Service through some common hydraulic problems on the ADVIA 120. This is a REVISED version which containsextensive changes to page 14 concerning troubleshooting probe clogs and conductivity problems.

Important Tips:When using this chart, the obvious has been eliminated. It assumes that on board Hydraulic Functions like System Wash and Flowcell Washes have been performed.

Remember to return devices and tubing to their original positions before proceeding to the next procedure. If using the exerciser and opening Valves, do a Valve Check to close all valves.

PRELIMINARY PRODUCT INFORMATIONControl Number: 024-0005-14Revision: A

Table of Contents

TROUBLESHOOTING: RBC PATHWAYS .......................................................................................................................................................................................................................TROUBLESHOOTING: PEROX PATHWAYS....................................................................................................................................................................................................................TROUBLESHOOTING: LEAKS at ASPIRATION MODES...............................................................................................................................................................................................TROUBLESHOOTING: CLOGS at ASPIRATION MODES................................................................................................................................................................................................TROUBLESHOOTING: CONDUCTIVITY PROBLEMS (PROBE CLOGS) ........................................................................................................................................................................TROUBLESHOOTING: IMPRECISION - SINGLE METHOD...........................................................................................................................................................................................TROUBLESHOOTING - DOME VALVES, REAGENT DIAPHRAGM.................................................................................................................................................................................CONTINUOUS IMPROVEMENT IDEAS ..........................................................................................................................................................................................................................


TROUBLESHOOTING: RBC PATHWAYS

COMPLAINT PATHWAYTESTED

STEPS TYPICALTIME

RESULT CORRECTIVE ACTION[If Pathway Tested Fails]

Rate flags inRBC/Baso/Retic Channels

No Results

1.UFCVacuum Test

1. Go to Exerciser.2. Access the Pneumatics Screen and verify

UFC and Main Vacuum readings.3. Open V5, V7, V10, and V13,4. Return to Pneumatics Screen and monitor

UFC and Main Vacuum.

Monitorfor at least1 min.

UFC Vacuum should not differfrom Main vacuum by morethan 5 in Hg.

Note: Capacity of VacuumPump should be 24"-25" Hg. Ifpump is compromised, thiscould cause vacuum problems.

1. Replace large vacushield on the waste jug. Checkfittings on waste jug including spigot and waste exitport.

2. Close all valves and allow vacuum to recover.3. Restart test.4. If test fails again, and system has an Auto-waste

configuration, bypass the in-line check valve in thewaste line to the waste jug.

5. Close all valves and allow vacuum to recover.6. Restart test.7. If test fails again, check for clogs in the waste lines

from the UFC and in the lines to and from the wastemanifold (behind power supply drawer). Clean out thewaste manifold.

8. Repeat test.

2.RBC Shuttleand Flowcell

1. Go to Exerciser.2. Dispense RBC reagent into reaction

chamber by putting Shear Valve inDispense Position and actuating V80.

3. Open V21, V9.4. Open V20 and measure time to empty the

chamber.

<11 sec. Measures pathways of V9, RBCsyringe ,CFM, V20, V21 andUFC Vacuum.

1. If UFC Vacuum Test Passes, perform RBC Shuttlepathway test to isolate.

2. If RBC shuttle pathway passes, do an F/C Wash.3. Repeat this test. If it still fails, go to RBC Shuttle

Pathway Test.4. If RBC Shuttle Pathway Passes, clean or replace the

CFM.

3.RBC ShuttlePathway

1. Go to Exerciser.2. Dispense RBC reagent into reaction

chamber by putting Shear Valve inDispense position and actuate V80.

3. Disconnect RBC flowcell sample line attop of sample syringe

4. Disconnect RBC flowcell shuttle line atthe bottom of flowcell and reconnect tonipple at the top of the sample syringe.

5. Open V21 and V9.6. Open V20 and measure time to empty the

< 8 sec. Measures pathways of V9,syringe, V20, V21, and UFCVacuum.

1. Fill a 10 ml syringe with 50% bleach and attach to theside of the sample syringe.

2. Open V20, V21 and allow vacuum to pull bleachthrough pathway. Close Valves. Go to ShuttleChamber drain if unable to complete.

3. Fill a 10 ml syringe with 50% bleach and attach toRBC/Baso sample line going into the UFC. Open V9,V10 and allow vacuum to pull bleach throughpathway. Close Valves.

4. Repeat this test.


chamber.

4.RBC ShuttleChamberDrain(V21)

1. Go to Exerciser.2. Connect 8" length of 0.081´tubing to RBC

Vacuum Shuttle chamber Vent.3. Submerge tubing in 10 ml of RBC

Sheath/Rinse.4. Open V21.5. Open V22 and measure the time to aspirate

10ml.

<10 sec. Measures pathways of V22,V21 and UFC Vacuum.

1. Attach syringe with 50% bleach to flowcell shuttle linegoing to the UFC.

2. Verify tubing is connected to shuttle vent and placedin overflow container.

3. Open V20 and V22.4. Open V21 and gently push bleach into RBC shuttle

Chamber.5. If necessary, let bleach soak with valves open and

compressor on for up to 1 hour. (Opening and closingV21 'pulsating' intermittently may also help.)


TROUBLESHOOTING: RBC PATHWAYS continued


STEPS TYPICAL TIME


5.RBC SheathDispense

1. Go to Exerciser.2. Disconnect sheath input to RBC sheath

syringe. (This is the tubing from V23 tosheath syringe.)

3. Actuate V34 to pressurize the RBC sheathdiaphragm pump.

4. Open V23.5. Open V19 and measure dispense time.

<35 sec. Measures pathways betweenUniversal Sheath/Rinse, DP5(V34), Sheath filter, V19,Flowcell, and V23

1. Verify there is no leakage of sheath filter and it is < 1month old. Change filter if suspect.

2. Do flowcell wash3. Verify connections at DP5. Verify DP5 and check

valves are working properly.4. Verify 5psi and 20” hg at DP5.5. Remove Sheath/Rinse straw and remove the filter.

Reinstall straw. Repeat this test. If the test passes,the filter is the problem.

6. If test does not pass, disconnect the tubing to theSheath/Rinse straw and insert tubing directly intoSheath/Rinse container. [Note: you will have to placestraw into container to overcome the level sensor.Prime Sheath/Rinse 5 times.

7. Repeat this test. If the test passes, the check valveinside the straw is the problem.

6.RBC/BasoSheathSyringe toWaste

1. Go to Exerciser.2. Remove the fitting going into V23.3. Attach a syringe filled with 10 ml of

Rinse/Sheath to the place you just removedthe fitting at V23.

4. Open V23.5. Open V27 and measure the time to aspirate

10 ml.

< 14 sec. Measures pathways of V23, V27,Waste Manifold, and Waste jug.

1. Verify V23 and V37 are opening.2. Verify tubing going to waste manifold behind the

power supply drawer is not constricted.3. Verify tubing from waste manifold to waste jug is not

constricted.4. Verify Waste Manifold does not have clog in it.


TROUBLESHOOTING: PEROX PATHWAYS


STEPS TYPICAL TIME


Rate flags inPerox Channel

PeroxCarryover

1.UFC VacuumTest






Note: Capacity of VacuumPump should be 24"-25" Hg.If pump is compromised, thiscould cause vacuum problems.





from the UFC and in the lines to and from the wastemanifold (behind power supply drawer). Clean outthe waste manifold.

8. Repeat test.

2.Perox Shuttleand Flowcell

1. Go to Exerciser.2. Manually fill Perox Chamber to very top

with Sheath/Rinse or DI Water.3. Open Valves 17, 5, 6.4. Open V16 and measure time to empty the

chamber.

<10 sec. Measures pathways betweenPerox Reaction chamber, V5,V6, CFM and V16 (shuttlechamber). Also UFC vacuum.

1. If UFC Vacuum Test Passes: Perform PeroxChamber Drain Test to isolate.

2. If Perox Chamber Drain Test Passes, do a PeroxShuttle Pathway Test.

3. If Perox Shuttle Pathway Test Passes, do a PeroxShuttle Chamber Test.

4. If Perox Shuttle Chamber Test passes, do a flowcellwash.

5. If necessary, clean, replace CFM.

3.PeroxChamber Drain


with Sheath/Rinse or DI Water.3. Open Valves 5.4. Open Valve 7 and measure time to empty

chamber.

< 3 sec. Measures pathway betweenPerox Reaction chamber andV7.

1. Fill Perox chamber with CN-Free Hgb Rgt.2. Open V5 and V7.3. Repeat twice.4. If necessary, remove chamber and clean drain port

according to Cleaning Perox Chamber Ports (ADV-H23).

4.Perox ShuttlePathway


with Sheath/Rinse or DI Water.

<1min. Measures pathways betweenPerox Sheath, DP1(V26),Sheath Filter, V15, flowcell

1. Go into Exerciser.2. Fill Perox chamber with CN-Free Hgb Rgt.3. Unscrew fitting from side of Perox sample syringe


3. Disconnect Perox flowcell sample line attop of sample syringe.

4. Disconnect Perox shuttle line at thebottom of flowcell and reconnect to nippleat the top of the sample syringe.

5. Open Valves 17, 5, 6.6. Open V16 and measure time to empty

chamber.

and V24. and attach to flowcell cleaning syringe.4. Open Valves 5,and 6,5. Push and pull syringe to flush CN-Free back and

forth. Open V7 to drain. Repeat flushing 2 times,allowing reagent to sit 10 minutes.

6. Flush final time and repeat with water. Repeat test.


TROUBLESHOOTING: PEROX PATHWAYS continued


STEPS TYPICALTIME


5.Perox ShuttleChamber Drain

1. Go to Exerciser.2. Connect 8 in. length of 0.081” tubing to

Perox Vacuum Shuttle Chamber VENT.3. Submerge tubing into 10 ml RBC

Sheath/Rinse or DI Water.4. Open V175. Open V18 and measure time to aspirate 10

ml.

<1 min. Measures pathway from Peroxshuttle to V17. Also UFCvacuum.

1. Go into Exerciser.2. Unscrew Perox sample tubing from side of block and

attach to flowcell cleaning syringe filled with CN-Free Hgb Rgt.

3. Open V16 and V18.4. Flush CN-Free back and forth. Open V17 to drain.

Repeat flushing 2 times, allowing reagent to sit for 10minutes.

5. Flush final time and repeat with water.

6.Perox SheathDispense

1. Go to Exerciser.2. Disconnect sheath input to Perox sheath

syringe. Place into small container.3. Actuate V26 to pressurize the Perox sheath

diaphragm pump.4. Open V24.5. Open V15 and measure dispense time.

<1 min. Measures pathways betweenUniversal Sheath/Rinse, DP1(V26), Sheath fllter, V15Flowcell and V24.

1. Verify there is no leakage of sheath filter and it is < 1month old.

2. Do flowcell wash.3. Verify connections at DP1. Verify DP1 and check

valves are working properly.4. Verify 5psi and 20” hg at DP1.

Verify that reagent filter to Perox Reagent bottle isnot clogged.

7.Perox SheathSyringe to Waste

1. Go to Exerciser.2. Remove the fitting going into V24.3. Attach a syringe filled with 10 ml of

Rinse/Sheath to the place you just removedthe fitting at V24.

4. Open V24.5. Open V25 and measure the time to aspirate

10 ml.

<17 sec Measures pathways of V24,V25, Waste Manifold, andWaste jug.

1. Verify V24 and V25 are opening.2. Verify tubing going to waste manifold behind the

power supply drawer is not constricted.3. Verify tubing from waste manifold to waste jug is not

constricted.4. Verify Waste Manifold does not have clog in it.


TROUBLESHOOTING: LEAKS at ASPIRATION MODES


STEPS TYPICALTIME


Leaks fromAutosamplerNeedle/CenteringCollar

1. CenteringCollar

2a.AutosamplerRinse VacuumLines - V44

2b.AutosamplerRinse VacuumLines - V45

1. Remove centering collar and check forclogs at centering collar nipples.

1. Go to Exerciser2. Disconnect tubing leading to V44 at the

Autosampler Centering Collar.3. Submerge open end of tubing in 10 ml of

RBC Sheath/Rinse.4. Open V44 and measure apiration time.

1. Go to Exerciser2. Disconnect tubing leading to V45 at the

Autosampler Centering Collar.3. Submerge open end of tubing in 10 ml of

RBC Sheath/Rinse.4. Open V45 and measure aspiration time.

<26 sec.

<23 sec.

Checks for clogs incentering collar.

Measures UFCVacuum, tubing toAS centering collar,pathway at UFC forV44.

Measures UFCVacuum, tubing toAS centering collar,pathway at UFC forV45.

1. Clean out ports and proceed to procedures below for checkingwaste lines.

1. If both tests fail, verify UFC Vacuum with UFC Vacuum Test.[Note: both V44 and V45 lines could be clogged or pinched.]

2. If one fails: verify tubing to specific valve is not clogged orpinched..

3. Try to aspirate 50% bleach into tubing and alternate with slugs ofair.

4. Remove coupling and verify that tubing connection at couplingis secure and that connector has no clog. Also, verify there is noclog at inlet of UFC.

5. With coupling removed, open specific valve and try to dispense50% bleach into valve area with alternate slugs of air.

6. If not successful, replace coupling and tubing and aspirate 50%bleach through specific valve. While bleach is being aspirated,close valve and allow bleach is sit to try to deteriorate clog.

7. Repeat test.

Leaks fromManual OpenTube Probe

Manual OpenTube SamplerVacuum Lineat Wash Block

1. Go to Exerciser.2. Remove sample probe.3. Remove Wash Block.4. Disconnect tubing leading to V4 from

Wash Block.5. Submerge open end of tubing in 10 ml of

RBC Sheath/Rinse.6. Open V4 and measure aspiration time.

<36 sec. Measures UFCVacuum, tubing fromWash Block to V4,pathway at UFC forV4.

1. Verify UFC Vacuum with UFC Vacuum Test.2. Verify tubing to V4 is not clogged or pinched.3. Verify nipple at wash block is not clogged.4. Aspirate 50% bleach into tubing and alternate with slugs of air.5. Remove coupling and verify that tubing connection at coupling

is secure and that connector has no clog. Also, verify there is noclog at inlet of UFC.

6. With coupling removed, V4 and try to dispense 50% bleach intovalve area with alternate slugs of air using transfer pipette..

7. If not successful, replace coupling and tubing and aspirate 50%bleach through specific valve. While bleach is being aspirated,close valve and allow bleach is sit to try to deteriorate clog.

8. Repeat test.

Leaks fromManual Closed

Manual ClosedTube Sampler

1. Go to Exerciser.2. Remove MCTS Needle Chamber.

<17 sec. Measures UFCVacuum, tubing from

1. Verify UFC Vacuum with UFC Vacuum Test.2. Verify tubing to V46 is not clogged or pinched.


Tube Needle Vacuum Lineat NeedleChamber

3. Disconnect line leading to V46 on sideport of Needle Chamber.

4. Submerge open end of line in 10 ml ofRBC Sheath/Rinse.

5. Open V46 and measure aspiration time.

the Needle Chamberto V46, pathway atUFC for V46.

3. Check side nipple on MCTS chamber is not clogged or tubingpinched.

4. Aspirate 50% bleach into tubing and alternate with slugs of air.5. Remove coupling and verify that tubing connection at coupling

is secure and that connector has no clog.6. With coupling removed, open V46 and try to dispense 50%

bleach into valve area with alternate slugs of air.7. If not successful, replace coupling and tubing and aspirate 50%

bleach through specific valve. While bleach is being aspirated,close valve and allow bleach is sit to try to deteriorate clog.

8. Repeat test.


TROUBLESHOOTING: CLOGS at ASPIRATION MODES


STEPS TYPICAL TIME


PoorAspirationfrom Any orAll Modes

PoorAspiration incertainSelectivity

Probe ClogsFailedAspiration

AspirationModes:Open

MCTS

Autosampler

Selectivity:

CBCCBC/DiffCBC/Diff/Retic

1. Go to Exerciser.2. Verify that V1 and V47 are closed.3. If testing open mode aspiration, use sample

probe and place selector valve in open mode.

If testing MCTS aspiration, use tubingattached to the bottom of the needle andplace selector valve in MCTS.

If testing AS aspiration, use tubing attachedto the bottom of the needle and placeselector valve in AS.

1. Submerge open tube probe in 10 ml of RBCSheath/Rinse.

2. Open V473. Open V72 for CBC, Open V72, V73 for CBC/Diff Open V72, V73, and V74 forCBC/Diff/Retic4. Open V1 and measure time to aspirate 10 ml.

<1min 50sec.

Measures UFC Vacuum,pathway from specificaspiration mode through shearface, clot filter, selector valve,and all associated tubing inpathway.

1. Verify UFC Vacuum with UFC Vacuum Test.2. Check the clot filter.3. By pass the selector valve by using syringe coupling to

connect the sample line from the UFC directly into thefitting containing the clot filter on the selector valve. [If significantly faster, troubleshoot selector valve forclogs or alignments.]

4. If bypassing the selector valve does not correct, cleanshear face off line and clean ports on rear shear face.

5. Repeat procedure for test using 50% bleach andalternating with slugs of air. Follow this with DIwater.

6. Repeat test.


Rinse DomeValve SealingTest

1. Go to Exerciser2. Adjust 40 psi down to 30 psi.3. Pressurize both rinse diaphragm pumps

(V28 and V29)4. Observe suspect rinse valves (V2, V8, V37,

V11, V66).5. Verify that rinse is not seeping past valve

into the chamber.


Checks for sealing of the domevalves

1. Check pilot pressure (40 psi) path from the pneumaticdrawer to the UFC.

2. Check small vacushield filter for integrity.3. Look for constrictions or leaks.4. If there is one valve in particular that is leaking (i.e.

rinse entering chamber during test) and it is not a domevalve failure, as a last resort, replace the pilot valve.

PoorAspiration orDripping Probe

UFC VacuumTest


UFC and Main Vacuum readings.





CompressorTimeout

Vacuum Out ofRange

3. Open V5, V7, V10, and V13,4. Return to Pneumatics Screen and monitor

UFC and Main Vacuum.Note: Capacity of VacuumPump should be 24"-25" Hg.If pump is compromised, thiscould cause vacuum problems.





8. Repeat test.


TROUBLESHOOTING: CONDUCTIVITY PROBLEMS (PROBE CLOGS)


STEPS TYPICAL TIME


Probe Clogs AspirationMode

1. Check for probe clogs in all aspirate modes. 2. Check for Aspiration time <20 sec. 3. Determine which detector is not functioning

properly by aspirating sample and watching greenlight and blood.

4. During the next aspiration, read voltage of

effected detector on Parallel Node brd.at the testpoints listed below before the sample reachesdetectors(dry) and when the sample contacts thesensors(wet). There are two failure modes.

Mode 1 - A sensor is conducting when the aspirationpath should be dry.

Mode 2 - A sensor is not conducting when theaspiration path has sample in it.

An alternate method to test conductance is to usethe exerciser. Go into the valves section and openvalves 1,47,72. Go into syringes pumps sectionand put the selector valve in the open position. Inthe pneumatics section move the shear valve tothe aspirate mode. You can now aspiratecontinuously from the open tube mode. Thus youcan aspirate while looking at the indicators screenin the exerciser. The conductors should bedetecting when the sample is present and notconducting when the line is dry.

Analog test points

Cond#1 = TP 9-4 &TP11-1gnd or

< 20 sec.

<20 sec.

N/A

1. Clog only in one mode. 2. > 20 SEC. 3. If light continues flashing, or

needle does not retract,Cond. #1 not detectingchange in state. If blood isaspirated straight to waste,Cond. #2 not detectingchange.

4. Mode 1A

A voltage of-0.5V to -5.0V ismeasured at the analog test pointwhen the path should be dry.

Mode 1BA voltage of less than -0.5v ismeasured at the analog test pointwhen the path is dry but the systemstill reports conductance.

Mode 2AA voltage of less than – 1v ismeasured at the analog test pointwhen the path has sample in it.Use a high control for worst case.

1. Go to CLOGS at ASPIRATION MODE 2. Go to CLOGS at ASPIRATION MODE 3. Go to Step 4. 4. Mode 1A

Determine if voltage is because Offset >0.6. Remove cable fromUFC to Scrambler brd. If voltage is >0.3, is v due to cable cross talkor grounding. If voltage <0.3 but goes back up when reconnectedrinse may be left in pathway(usually this voltage will decrease asrinse “dries”). Do UFC Vacuum test. If passes, do Rinse DomeValve Sealing test. Check for leaks in the waste system. Clean outsample pathway with bleach solution. Consult TTN before changingUFC. Mode 1BMake sure threshold of 0.6v is set at threshold TP’s. Check forsignal transition at TP9-3 or TP11-3. This is the digitalsignal(TTL) out for conductance. It should toggle when theanalog signal at TP9-4 or TP11-4 passes through –0.6v. If notthe parallel node board is suspect.

Mode 2ACheck cable continuity. Make sure pins in cable connectors aremaking good contact. Check version of parallel node bd. Re-tightenscrews going to the conductivity sensor on the back of the UFC.Note serial # and mfg of UFC. Clean out sample pathway across


U9 pin 8 & TP11-1 gndCond#2 = TP11-4 & TP11-1 gnd or U12 pin1 & TP11-1 gndThreshold test points

Cond#1 TP9-2 and gndCond#2 TP11-2 and gnd

Mode 2BA voltage greater than –1v isdetected at the analog test pointbut the system does not detectconductance.

detector with bleach solution. Consult TTN before changing UFC.

Mode 2BRepeat as in mode 1B.

UFC VacuumTest





UFC Vacuum should not differfrom Main vacuum by morethan 5 in Hg as both vacuumsdrop.

Note: Capacity of VacuumPump should be 24"-25" Hg.If pump is compromised, thiscould cause vacuum problems.






8. Repeat test.


Rinse DomeValve SealingTest

1. Adjust 40 psi down to 30 psi2. Go to Exercisor.3. Pressurize both rinse diaphragm pumps

(V28 and V29)4. Observe suspect rinse valves (V2, V8, V37,

V11, V66).5. Verify that rinse is not seeping past the

valve into the chamber.



1. Check pilot pressure (40 psi) path from the pneumaticdrawer to the UFC.

2. Check small vacushield filter for integrity.3. Look for constrictions or leaks.4. If there is one valve in particular that is leaking (i.e.

rinse entering chamber during test) and it is not a domevalve failure, as a last resort, replace the pilot valve.


TROUBLESHOOTING: IMPRECISION - SINGLE METHOD


STEPS TYPICAL TIME


Imprecision fora SingleMethod

1.ReactionChamber / Ventfor SpecificMethod

1. Go to Exerciser.2. For Perox problem use E-Z Kleen.

For RBC/Baso/Retic problem us 50%bleach.

3. Open the appropriate waste valve for thespecific method: Perox -V5, V6, V7. RBC - V-10, Baso - V13, Retic - V65.Aspirate the appropriate fluid through thevent for the method and drain line. Checkvent lines for pinches. Check Perox cap forclog.

Cleans chamber pathwayincluding vent and waste foreffected method.

1. Repeat Precision after this test.2. If not corrected go to next procedure.

2.ReagentDelivery forSpecificImpreciseMethod

1. Locate a magnifying glass or loupe.2. Locate the sample delivery and reagent

delivery line for the method in question.3. Trace the reagent delivery back to the

reagent bottle4. Prime the reagent line using the hydraulic

prime cycles.5. With the magnifying glass, examine the

reagent delivery to the chamber.

No air or bubbles should be seenin the reagent lines during primecycles.

Note: If imprecision isselectivity specific, check for airleak or clogging in specificmode.

1. If the bubbles are seen rising up from the shear face,trace the line back to the line from the reagent pumpto the shear face.

2. If no bubbles are seen in #1 then clean the shearface.

3. If bubbles are seen in # 1, then examine the reagentpump as it fills from the prime cycles. If bubbles areinjected, check the reagent line for leaks. Swap endsof the reagent line to the reagent pump and reagentbottle. Prime several times to allow bubbles to clear.

4. If this does not correct, remove reagent fitting fromstraw filter. Place fitting directly into reagent to seeif filter on straw is the problem.

5. Repeat test.

3.Noise Causedby ImproperGrounding

1. Run Saline samples in the Autosamplermode.

2. Look for significant noise in the cytogramof the method in question.

Minimum noise should be seenin any channel.

1. If noise is present, block the light path.2. If noise is still present with the light path blocked,

check that the system ground strapping is proper.

4.Leak from11-PositionManifold

1. Go to Exerciser.2. Exercise the reagent pump associated with

the method while monitoring vacuum and

Should not see a significant dropin pressure or vacuum (>2-3 psior >1-2"Hg). If a small drop is

1. Access the 11 position manifold and pinch off thepressure vacuum line that goes to the reagent pump.

2. Repeat test.


pressure readings from the pneumaticscreen.

seen, it should recover whilepump is still exercised. Largedrop in pressure or vacuumand/or failure to recoverindicates a leak associated withthe 11 position manifold,pneumatic valve, or at tubing tospecific pump or manifold.

3. If results are good, the leak is in the line to thepump.

4. If the results are not good, then swap the pneumaticvalve with another position to isolate the problem tothe valve itself.

5. Cross check by testing the valve in its new position.


TROUBLESHOOTING: IMPRECISION - SINGLE METHOD continued


STEPS TYPICALTIME


Imprecision fora SingleMethod cont.

5.Rinse DomeValve SealingTest

1. Go to Exerciser2. Adjust 40 psi down to 40 psi.3. Pressurize both rinse diaphragm pumps

(V28 and V29)4. Observe suspect rinse valves for the method

in question: (V2, V8, V37, V11, V66).5. Verify that rinse is not seeping past valve

into the chamber.

Monitor forat least 1min.


1. Check pilot pressure (40 psi) path from thepneumatic drawer to the UFC.

2. Look for constrictions or leaks.3. If there is one valve in particular that is leaking

(i.e. rinse entering chamber during test) and it isnot a dome valve failure, as a last resort, replacethe pilot valve.

HgbImprecision

1. Turn the 20 psi down to 14 psi.2. Run 10 to 20 sample precision.3. Examine only the HGB precision.

Disregard all other parameters.4. If precision is improved and "Flatness" is

good, then violent mixing in the Hgbchamber is suspect.

Perform this test if no significantbubbles are observed in thereagent line and the transmissionhas a curve at the beginning ofthe sample portion of the Hgbhistogram.

1. If failure seen at 20 psi but improved at 14 psi thenproblem could be incorrect entry angle of sample.

2. # 1 could be due to Obstruction in Sample Line -Troubleshoot this pathway

3. # 1 could be due to Air Leak in Reagent Line -Troubleshoot this pathway.

4. Raise pressure to 19.5. If sample portion of theHgb histogram is still good when running samples,re-run precision.


TROUBLESHOOTING - DOME VALVES, REAGENT DIAPHRAGM


STEPS TYPICAL TIME


Loss of Vacuumat UFC

Caused byPilot Valve

Caused byDome Valve

UFC DomeValve andPilot Valveintegrity

1. If dome valve failure is suspected, verify thatthe pilot valve associated with this domevalve is functioning.

2. Go to Exerciser.3. In the Valves Screen, open the valve for the

suspected failure. If the Valve on screenremains green while exercised, then thesystem is registering the correct electricalresponse.

4. If the Valve shows red, then the indication isthere is an electrical failure.

5. Troubleshoot by swapping the Valve DriverBoards to see if the problem moves.

6. If the Valve Driver swap does not correct,then

replace the suspect pilot valve according to the procedure in the Service Guide.

1. Check to see if Pilot valves vacuum supply(middle rear) has fluid in it.

2. Check to see if there is fluid at the back ofthe UFC coupling (pull out Pnematics drawerto view) in the tubing from port 15 to thewaste manifold.

Test to see if Pilot Valve is theproblem for Dome Valve Failure.

No fluid should be seen in thePilot valve vacuum line from theUFC to the UFC coupling

1. If this test does not fail, then go to loss of Vacuumcaused by Dome Valve.

1. If fluid is seen in the Pilot Valve vacuum line

from the rear of the UFC, then the integrity of oneof the dome valves is compromised and the UFCshould be changed.


Caused byDiaphragmPumpFailure/Leak

DiaphragmPumpIntegrity

1. Verify integrity of each diaphragm pump.2. If a pump is found faulty and fluid has

invaded the vacuum system, then the smallvacushields will be wet.

Note: these vacushield filters are designed to holdback moisture. If a large amount of fluid isexperienced, then it will penetrate these filters

No fluid should be seen in thevacuum/pressure lines from thediaphragm pump to the 11-position manifold.Note: If a leak is experienced in adiaphragm pump, then any of thevacuum lines to the 11- positionmanifold can have fluid in themsince the vacuum supply is

1. If a diaphragm pump is found to be faulty, thenreplace it with a new one.

2. The vacuum lines involved will have to be driedout and vacushields replaced.

3. Note: Additional parts may have to be replaced iffluid did invade, including the 11-positionmanifold, vacuum regulator, vacuum head andpressure node board.


universal.


Caused byReagent PumpFailure/Leak

ReagentPumpIntegrity

1. Verify integrity of each reagent diaphragmpump.

2. If a pump is found faulty and fluid hasinvaded the vacuum system, then the smallvacushields will be wet.

Note: these vacushield filters are designed to holdback moisture. If a large amount of fluid isexperienced, then it will penetrated these filters.

No fluid should be seen in thevacuum/pressure lines from thediaphragm pump to the 11-position manifold.Note: If a leak is experienced in adiaphragm pump, then any of thevacuum lines to the 11- positionmanifold can have fluid in themsince the vacuum supply isuniversal.

1. If a reagent pump is found to be faulty, thenreplace it.

2. The vacuum lines involved will have to be driedout and vacushields replaced.

3. Note: Additional parts may have to be replaced iffluid did invade, including the 11-positionmanifold, vacuum regulator, vacuum head andpressure node board.


CONTINUOUS IMPROVEMENT IDEAS

Use this page to keep track of ideas for change as you think of them.Forward your suggestions to Pat Frank: By e-mail: Patricia Frank/TRYTN/DIAG/US/BAYER,Or Fax 1-91524-2249Thank you for contributing!

advia 120 troubleshooting guide

Documents

putting shear

dispense rbc

main vacuum

dispense position

conductivity

measure time

aspiration

open v21