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4559120

PATENT ABSTRACTS

A G A R O S E G E L E L E C T R O P H O R E S I S T E C H N I Q U E

FOR T H E D E T E R M I N A T I O N OF A M Y L A S E I S O E N Z Y M E S

Vicki L Royse, Donald M Jensen assigned to Rush-Presbyterian-St Luke's Medical Center

Separation of all clinically relevant isoamylase bands including PI PIb, P2, P3, P4. SI, $2, $3, $4 and $5 is achieved in a test in which a bio- logical sample is electrophoresed on an agarose gel supporting a tris-sodium barbital-barbital buffer at a pH of between 8.4 and 9.2. The bar- bital anion concentration is between about 0.03 and about 0.08 M. the tris cation concentration is between about 0.03 M and about 0.07 M; and the sodium cation concentration is between ab- out 0.03 M and about 0.07 M. Agarose is present in the gel at concentrations of between about 0.4 and about 1.5 weight percent.

mits potential anti-allergy agents to be assayed in a number of ways. For example, the binding and dissociation rates of lgE to the mast cells in the presence and the absence of the substance being tested may be measured thereby giving a direct indication of that substance's ability to interfere with the IgE binding reaction. Another measure ofa substance's potential as an anti-allergy agent is based upon the release of mediators or other compounds from the mast cells after sensitiza- tion by the allergen and exposure of the sensitized cells to the allergen. The present inven- tion generally involves the following steps: (a) sensitizing cloned mast cells to an allergen: (b) exposing sensitized mast cells to the allergen in the presence of a test anti-allergy agent: and (c) measuring the reaction products of step (b) for an indication of test compound effect.

4560504

CARBOXYL ANCHORED IMMOBILIZED ANTIBODIES

4559299

C Y T O T O X I C I T Y A S S A Y S IN C E L L C U L T U R I N G D E V I C E S

M Boris Rotman assigned to Brown University Research Foundation Inc

Methods and devices for assaying the sensitivity of of biopsied cells to therapeutic agents are dis- closed. Cells are cultured in artificial organs and then contacted with a fluorogenic substrate such that living cells accumulate a characteristic amount of fluorescence. The agent is then in- troduced into the organ and changes in the fluorescence released by the cells serve as an in- dicator of the sensitivity of the cells to the agent.

Edward C Arnold assigned to UOP lnc

An immobilized antibody system can be made by reacting an aminated core support with an antibody in the presence of a condensing agent which promotes the formation of the amide linkage. The immobilized antibody system is highly resistant to leaching, may be made in- compressible, sterilizable, and pyrogen-free. Such an immobilized antibody system is well suited for repeated use with minimal change in its physical and biochemical properties.

4560648

HOMOGENEOUS ENZYME IMMUNOASSAY FOR FERRITIN

4559310

A S S A Y M E T H O D S A N D S Y S T E M S U T I L I Z I N G M A S T C E L L C L O N E S

Harvey I Cantor, Gary Nabel assigned to Dana Farber Cancer Institute

The present invention is directed to an in vitro assay, useful in determining the effectiveness of anti-allergy compounds and'or useful in measuring the degree of sensitivity of a patient to particular allergens. The present invention per-

Richar Armenta assigned to Syntex (U S A ) Inc

Composition and method are provided for en- zyme immunoassays for high molecular weight proteins, such as ferritin. An enzyme is con- jugated to the high molecular weight protein through a specific linking group. Usually, on the average, about 2 to 10 enzyme molecules are bonded to each protein molecule. In an assay, the conjugate competes with an unknown sam- ple for receptor and the resulting enzymatic ac- tivity is compared to a standard for a determination of the presence and amount of protein in the unknown.