04 infeksi saluran napas
TRANSCRIPT
INFEKSI SALURAN NAPAS
DICKY BAGUS WIDHYATMOKO
25 – 26 MARET 2015DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERANUNIVERSITAS AIRLANGGA
DISEDIAKAN
Streptococcus : S. viridans
S. pneumoniae
S. pyogenes
Corynebacterium spp
M. tuberculosis
Gram Stain
Blood Agar
Chocolate Agar
Optochin Test
Bacitracin test
PAI medium
Neisser Stain (AB, C)
Ziehl-Neelsen Stain
prepared slide
Identifikasi Streptococcus spp.
Blood Agar
Chocolate Agar
Optochin Test
Bacitracin test
Zona hemolisis
Antibiotic Sensitivity Test for Identification
First step
Gram Stain
always do gram stain before continuing to others test
Catalase Test
please learn from previous laboratory practice
hemolysis
partial / incomplete hemolysis
reduction red blood cell hemoglobin methemoglobin in the medium surroundingthe colony.
The greenish color is caused by the presenceof biliverdin, which is a by-product of thebreakdown of hemoglobin
e.g. S. pneumoniae, S. viridans groups
hemolysis
complete or true lysis of red blood cells
clear zone, approaching the color andtransparency of the base medium, surroundsthe colony
e.g. S. pyogenes
hemolysis
how to differentiate between S. pneumoniaewith S. viridans
Using
Optochin test
Inulin Test
Bile Solubility Test
Optochin Test
Antibiotics Susceptibility Test
Optochin also known as ethyl-hydro-cupreine
To Differentiate between S. pneumoniae withS. viridans
S > 14 mm : S. pneumoniae
No zone inhibition : S. viridans
Optochin Test
taken from http://arabslab.com
S. pneumoniae Sensitive
S. viridans groups no zone inhibition
Inulin Test
To differentiate between Streptococcuspneumoniae with all others alpha-hemolyticsstreptococci
pneumoniae hydrolize inulin
Bile Solubility Test
Bile (sodium deoxycholate)
Distinguishes S. pneumoniae from all others alpha-hemolytics streptococci
Sodium deoxycholate (2% in water) will lyse the pneumococcal cell wall
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