year: second year semester: i ii course code: xmi301 ......ii. riboflavin (vitamin b2) and the...

14
Year: Second Year Course: Bacterial Physiology a Teaching Scheme (Hrs/Week) Continuous In L T P C CIA-1 CIA-2 4 0 - 4 10 20 Max. Time, End Semester Exam Prerequisite Introduct Objectives 1 To understand the structu cells 2 To understand the modes 3 To learn about biochemic bacterial bioenergetics 4 To impart the knowledge 5 To know the principles o Unit Number 1 Fundamentals of Ca 1. Carbohydrate: Monosaccharides, Mutarotation, stru 2. Lipid: importance acids, storage lipid 3. Proteins: importan zwitterions natur Introduction to sec 4. Role of cofactors Occurrence, Struc i. Nicotinic A ii. Riboflavin iii. Thiamine iv. Pantotheni v. Pyridoxal vi. Metal ion 2 Bacterial Physiology 1. Metabolism, catabo 2. Glucose degradi phosphoketolase pathw 3. Pyruvate oxidizing 4. Interconnectivity of School of Science B.Sc. Microbiology Semeste and Biochemistry Course C nternal Assessment (CIA) End Semester Examination 2 CIA-3 CIA-4 Lab Theory Lab 10 10 - 50 - m (Theory) - 3Hrs. tory concepts of microbiology ure and function of building block chemical co s of microbial metabolism cal mechanisms of ATP synthesis and electron e of enzymes and its role in biological system f instruments used in study of microbial physiol Details arbohydrates, Lipids, Proteins, Vitamins and importance and classification. Struct , reducing and oxidizing properties of Monosa ucture of Disaccharides and polysaccharides e and classification. Structures and propertie ds and membrane lipids nce of proteins and classification. Structures, tit re of amino acids, structural organization of condary metabolites in metabolism: cture and Biochemical functions of the following Acid (Niacin) and the Pyrimidine nucleotides. n (Vitamin B2) and the Flavin nucleotides (Vitamin B1) and Thiamine Pyrophosphate ic acid and coenzyme A phosphate (Vitamin B6) ns y olism, anabolism, respiration, fermentation ing pathways Glycolysis, HMP, ED way. pathway – TCA (with emphasis on amphibolism f pathways carbohydrates, proteins, lipids, nuc Page1 er: III Code: XMI301 Total 100 ompounds of living transfer process in logy Hours minerals tures of accharides, es of fatty tration and f proteins. g: 12 pathway, m) cleic acids 12

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Year: Second Year

Course: Bacterial Physiology and Biochemistry

Teaching

Scheme

(Hrs/Week)

Continuous Internal

L T P C CIA-1 CIA-2

4 0 - 4 10 20

Max. Time, End Semester Exam (Theory)

Prerequisite • Introduct

Objectives

1 To understand the structure and function of building block

cells

2 To understand the modes of microbial metabolism

3 To learn about biochemical mechanisms of ATP synthesis

bacterial bioenergetics

4 To impart the knowledge of enzymes and its role in biological system

5 To know the principles of

Unit

Number

1 Fundamentals of Carbohydrates, Lipids, Proteins, Vitamins and minerals

1. Carbohydrate: importance and classification. Structures of

Monosaccharides, reducing and oxidizing properties of Monosaccharides,

Mutarotation, structure of Disaccharides and polysaccharides

2. Lipid: importance and classification. Structures and properties of fatty

acids, storage lipids and membrane lipids

3. Proteins: importance of

zwitterions nature of amino acids, structural organization of proteins.

Introduction to secondary metabolites

4. Role of cofactors in metabolism:

Occurrence, Structure and Biochemical functions of the following:

i. Nicotinic Acid (Niacin) and the Pyrimidine nucleotides.

ii. Riboflavin (Vitamin B2) and the Flavin nucleotides

iii. Thiamine (Vitamin B1) and Thiamine Pyrophosphate

iv. Pantothenic acid and co

v. Pyridoxal phosphate (Vitamin B6)

vi. Metal ions

2 Bacterial Physiology

1. Metabolism, catabolism, anabolism, respiration, fermentation

2. Glucose degrading pathways

phosphoketolase pathway.

3. Pyruvate oxidizing pathway

4. Interconnectivity of pathways

School of Science

B.Sc. Microbiology

Semester: I

Physiology and Biochemistry Course Code:

Continuous Internal Assessment (CIA) End Semester

Examination

2 CIA-3 CIA-4 Lab Theory Lab

10 10 - 50 -

End Semester Exam (Theory) - 3Hrs.

Introductory concepts of microbiology

To understand the structure and function of building block chemical compounds

modes of microbial metabolism

biochemical mechanisms of ATP synthesis and electron transfer process in

To impart the knowledge of enzymes and its role in biological system

rinciples of instruments used in study of microbial physiology

Details

Fundamentals of Carbohydrates, Lipids, Proteins, Vitamins and minerals

Carbohydrate: importance and classification. Structures of

, reducing and oxidizing properties of Monosaccharides,

Mutarotation, structure of Disaccharides and polysaccharides

Lipid: importance and classification. Structures and properties of fatty

acids, storage lipids and membrane lipids

Proteins: importance of proteins and classification. Structures, titration and

zwitterions nature of amino acids, structural organization of proteins.

Introduction to secondary metabolites

cofactors in metabolism:

Occurrence, Structure and Biochemical functions of the following:

Nicotinic Acid (Niacin) and the Pyrimidine nucleotides.

Riboflavin (Vitamin B2) and the Flavin nucleotides

Thiamine (Vitamin B1) and Thiamine Pyrophosphate

Pantothenic acid and coenzyme A

Pyridoxal phosphate (Vitamin B6)

Metal ions

Bacterial Physiology

Metabolism, catabolism, anabolism, respiration, fermentation

Glucose degrading pathways – Glycolysis, HMP, ED pathway,

phosphoketolase pathway.

oxidizing pathway – TCA (with emphasis on amphibolism)

. Interconnectivity of pathways – carbohydrates, proteins, lipids, nucleic acids Pa

ge1

Semester: III

Course Code: XMI301

End Semester

Total

100

compounds of living

lectron transfer process in

n study of microbial physiology

Hours

Fundamentals of Carbohydrates, Lipids, Proteins, Vitamins and minerals

Carbohydrate: importance and classification. Structures of

, reducing and oxidizing properties of Monosaccharides,

Lipid: importance and classification. Structures and properties of fatty

proteins and classification. Structures, titration and

zwitterions nature of amino acids, structural organization of proteins.

Occurrence, Structure and Biochemical functions of the following:

12

Glycolysis, HMP, ED pathway,

amphibolism)

carbohydrates, proteins, lipids, nucleic acids

12

5. Concept of central and peripheral energy yielding pathways

6.Oxidative phosphorylation and substrate level

chemiosmotic hypothesis of ATP formation

7. Pathways for fermentation of ethanol and lactic acid

heterofermentative

3

Bioenergetics:

1. Fundamental principles

concept, high energy compounds.

2. ATP generation by :

i) Substrate level phosphorylation.

ii) Oxidative phosphorylation

anaerobic respiration.

iii) Bacterial Photophosporylation

Photophosporylation in purple bacteria and Non

in cyanobacteria.

4 Enzymes :

1. Classification

2. Factors influencing enzyme activity (

pH, metal ions)

3. Regulation of enzyme activity

feedback inhibition.

4. Applications of enzymes : Amylases, proteases and lipases

5 Principles of instruments used i

1. Centrifugation techniques

ultracentrifugation and applications

2. Chromatography techniques

and affinity chromatography with applications

3. Spectrophotometric techniques

4. Electrophoretic techniques

5. Radiobiological techniques

radioisotope dilution techniq

6. Manometric techniques

Course Outcome

Students should able to

CO1 Student will be able to d

compounds of biological

CO2 Student will be able to demonstrate the basic

microbes

CO3 Student will be able to demonstrate the basic idea

production purpose

CO4 Student will be able to understand the

microbial physiology

Resources

Recommended

Books 1. General Microbiology

2. A Text book of microbiology

Ltd. Ramnagar New

Concept of central and peripheral energy yielding pathways

Oxidative phosphorylation and substrate level phosphorylation ,

chemiosmotic hypothesis of ATP formation

Pathways for fermentation of ethanol and lactic acid - homofermentative and

Fundamental principles of energetics, importance of Gibbs free energy

energy compounds.

. ATP generation by :

Substrate level phosphorylation.

phosphorylation - Respiration electron transport chain, aerobic and

anaerobic respiration.

Photophosporylation – Photosynthetic apparatus, Cyclic

Photophosporylation in purple bacteria and Non-cyclic Photophosporylation

influencing enzyme activity (Substrate concentration, temperature,

. Regulation of enzyme activity: Concept of allosteric enzymes and pattern of

inhibition.

4. Applications of enzymes : Amylases, proteases and lipases

Principles of instruments used in study of microbial physiology:

Centrifugation techniques –Angular velocity, RPM Vs ‘g’ relations and

ultracentrifugation and applications

Chromatography techniques – Partition, adsorption, ion exchange, exclusion

hromatography with applications

Spectrophotometric techniques – UV and visible

Electrophoretic techniques – agarose and polyacrylamide gel

Radiobiological techniques –characters of radioisotopes, Autoradiography,

radioisotope dilution technique and pulse chase experiments

Manometric techniques – Warburg’s respirometer

Student will be able to demonstrate an understanding of major building block chemical

compounds of biological system

Student will be able to demonstrate the basic understanding of metabolic pathways of

Student will be able to demonstrate the basic idea of biochemical pathways for

Student will be able to understand the principles of instruments used in study of

1. General Microbiology – Vol. I and Vol. II – Pawar and Daginawala

2. A Text book of microbiology – R. Dubey, D. K. Maneshwari, S. Chand Co.

Ltd. Ramnagar New Delhi 110055

Pa

ge2

phosphorylation ,

homofermentative and

of energetics, importance of Gibbs free energy

Respiration electron transport chain, aerobic and

aratus, Cyclic

cyclic Photophosporylation

12

Substrate concentration, temperature,

losteric enzymes and pattern of

12

Angular velocity, RPM Vs ‘g’ relations and

Partition, adsorption, ion exchange, exclusion

characters of radioisotopes, Autoradiography,

12

Total 60

emonstrate an understanding of major building block chemical

metabolic pathways of

of biochemical pathways for

principles of instruments used in study of

Pawar and Daginawala

R. Dubey, D. K. Maneshwari, S. Chand Co.

3. Microbiology

4. Fundamentals of Microbiology

Reference Books 1. Biochemistry

2. Outlines of Biochemistry

3. Microbiology – Pelczar, Reid and Chan

4. Fundamentals of Microbiology – Frobisher et al.

1. Biochemistry – Lehninger.

2. Outlines of Biochemistry – Cohn and Stumph

Pa

ge3

Year: Second Year

Course: Bacterial Physiology and Biochemistry

Teaching

Scheme

(Hrs/Week)

Continuous Internal Assessment (CIA)

L T P C CIA-1 CIA-

2

0 0 4 2 - -

Max. Time, End Semester Exam (Theory)

Objectives

1 To impart the knowledge of biochemical pathways of microorganisms

2 To train the students in biochemical characterization of microorganisms for identification

3 To understand physiological parameters of microorganisms

Sr.

No.

1 Growth curve - Use absorbance measurement for bacterial culture and (Calculation of

growth rate, specific growth rate and generation time)

2 Biochemical characterization of bacteria

Sugar utilization test (minimal medium + sugar)

3 Sugar fermentation test

4 IMViC

5 Enzyme detection – Amylase

6 Gelatinase test

7 Catalase test

8 Oxidase test

9 Oxidative-fermentative test

10 Identification of any two bacterial isolates

11 Paper chromatography

12 Industrial visit

School of Science

B.Sc. Microbiology

Semester: I

Physiology and Biochemistry Laboratory Course Code:

Continuous Internal Assessment (CIA) End Semester

Examination

CIA-

3

CIA-

4 Lab Theory Lab

- - 50 - 50

End Semester Exam (Theory) - 2Hrs.

To impart the knowledge of biochemical pathways of microorganisms

To train the students in biochemical characterization of microorganisms for identification

understand physiological parameters of microorganisms

Description

Use absorbance measurement for bacterial culture and (Calculation of

growth rate, specific growth rate and generation time)

Biochemical characterization of bacteria

(minimal medium + sugar)

Sugar fermentation test

Amylase production test

fermentative test

of any two bacterial isolates at least up to genus level.

Semester: III

Course Code: XMI311

Total

100

To train the students in biochemical characterization of microorganisms for identification

Use absorbance measurement for bacterial culture and (Calculation of

Term Work:

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

continuous assessment based on

journal/record book, oral/viva, respectively

the end of the semester, the final grade for a Term Work shall be assigned based on the performance of

the student and is to be submitted to the University.

Notes

1 The regular attendance of the

marks will be given accordingly (10

2 Good Laboratory Practices (

3 Timely Completion (10 Marks)

4 Journal / Record Book (10 Marks)

5 Oral / Viva (10 Marks)

Practical/Oral/Presentation:

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

appointed as internal and external

mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope

shall be submitted to the head of the department or authorized person.

Notes

1 One experiment from the regular practical syllabus will be conducted (40 Marks).

2 Oral/Viva-voce (10 Marks).

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

continuous assessment based on attendance, good laboratory practice (GPL), timely completion,

journal/record book, oral/viva, respectively. It should be assessed by course teacher of the institute. At

the end of the semester, the final grade for a Term Work shall be assigned based on the performance of

the student and is to be submitted to the University.

the students during semester for practical course will be monitored and

rks will be given accordingly (10 Marks).

Good Laboratory Practices (10 Marks)

Timely Completion (10 Marks)

Journal / Record Book (10 Marks)

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

internal and external examiners by the University. The examiners will prepare the

as specified by the University, authenticate and seal it. Sealed envelope

shall be submitted to the head of the department or authorized person.

One experiment from the regular practical syllabus will be conducted (40 Marks).

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

attendance, good laboratory practice (GPL), timely completion,

teacher of the institute. At

the end of the semester, the final grade for a Term Work shall be assigned based on the performance of

practical course will be monitored and

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

The examiners will prepare the

as specified by the University, authenticate and seal it. Sealed envelope

One experiment from the regular practical syllabus will be conducted (40 Marks).

Year: Second

Course: Biotechnology

Teaching

Scheme

(Hrs/Week)

Continuous Internal Assessment (CIA)

L T P C CIA-1 CIA-2

4 0 - 4 10 20

Max. Time, End Semester Exam (Theory)

Prerequisite Students should have the fundamental knowledge of applied Biology.

.Objectives

1 To understand the basic Biotechnology and its scope

2 To understand Plant Tissue

3 To understand Fermentation technology and its application.

4 To understand bio fuels, their production and uses.

Unit

Number

1

Introduction: Introduction to Biotechnology

Definition, scope and multidisciplinary nature.

Biotechnology in India

Social, Moral and Ethical issues

Intellectual Property Rights: Patents and Copyright

2

Plant Tissue Culture:

Totipotency. Nutrient Media Composition, Designing of tissue culture laboratory

Callus Culture, Embryo Culture, Suspension culture, r

hardening. Somatic hybridisation: Protoplas

Seeds. Concept of Transgenic plants and GMOs. Application of Tissue culture

techniques in Agriculture. Introduction to Nanotechnology.

3

Fermentation Technology

Organisms involved, substrates used, products and by products, Concept of

scaling up. Cultures: Batch and continuous. Types of bioreactors: Tubular tower

bioreactor, digestive bioreactor, stirred tank bioreactor. Manufacture of citric acid

and pencillium.

4

Biofuels: Introduction to biofuels, advantages and disadvantages. Concept of

non-conservational energy resources.

Bioenergy resources: petrocrops, wood, sugar and sugar crops.

Biogas production: substrate used, digester, microorganisms involved, process

characters, factors effecting gas yield, precautions, advantages and disadvantages.

Production of bioethanol and biodiesel.

5

Environmental Biotechnology:

Principle of water waste treatment: physic

anaerobic methods.

School of Science B.Sc. Microbiology

Semester: I

Course Code:

Continuous Internal Assessment (CIA) End Semester

Examination

2 CIA-3 CIA-4 Lab Theory Lab

10 10 - 50 -

End Semester Exam (Theory) - 3Hrs.

Students should have the fundamental knowledge of applied Biology.

To understand the basic Biotechnology and its scope

To understand Plant Tissue Culture techniques

To understand Fermentation technology and its application.

To understand bio fuels, their production and uses.

Details

Introduction to Biotechnology

and multidisciplinary nature.

Biotechnology in India.

ocial, Moral and Ethical issues

Intellectual Property Rights: Patents and Copyright

Plant Tissue Culture: History, Introduction and importance, Concept of

Nutrient Media Composition, Designing of tissue culture laboratory

Callus Culture, Embryo Culture, Suspension culture, regeneration and plant

Somatic hybridisation: Protoplast Isolation and Fusion.

. Concept of Transgenic plants and GMOs. Application of Tissue culture

techniques in Agriculture. Introduction to Nanotechnology.

Fermentation Technology: Definition with an example and importance

Organisms involved, substrates used, products and by products, Concept of

scaling up. Cultures: Batch and continuous. Types of bioreactors: Tubular tower

bioreactor, digestive bioreactor, stirred tank bioreactor. Manufacture of citric acid

Introduction to biofuels, advantages and disadvantages. Concept of

conservational energy resources.

Bioenergy resources: petrocrops, wood, sugar and sugar crops.

Biogas production: substrate used, digester, microorganisms involved, process

characters, factors effecting gas yield, precautions, advantages and disadvantages.

Production of bioethanol and biodiesel.

Environmental Biotechnology: Introduction: type of wastes and their resources.

Principle of water waste treatment: physic-chemical characteristics, aerobic and

Pa

ge1

Semester: III

Course Code: XBO301

Total

100

Students should have the fundamental knowledge of applied Biology.

Hours

12 L

History, Introduction and importance, Concept of

Nutrient Media Composition, Designing of tissue culture laboratory

egeneration and plant

on and Fusion. Synthetic

. Concept of Transgenic plants and GMOs. Application of Tissue culture

12 L

Definition with an example and importance.

Organisms involved, substrates used, products and by products, Concept of

scaling up. Cultures: Batch and continuous. Types of bioreactors: Tubular tower

bioreactor, digestive bioreactor, stirred tank bioreactor. Manufacture of citric acid

12 L

Introduction to biofuels, advantages and disadvantages. Concept of

Biogas production: substrate used, digester, microorganisms involved, process

characters, factors effecting gas yield, precautions, advantages and disadvantages.

12 L

Introduction: type of wastes and their resources.

chemical characteristics, aerobic and 12 L

Course Outcome

Students should be able to

CO1 Students will understand the basic Biotechnology and its scope

CO2 Students will understand Plant Tissue Culture techniques and their applications

CO3 Students will understand Fermentation technology and its application.

CO4 Students will understand bio fuels, their production and uses.

Solid waste management, physic

nonhazardous wastes, biodegradable and nonbiodegradable wastes, collection and

transport of solid wastes.

Disposal methods of solid wastes with special references to landfill, secured

landfill, biological compositing.

Concept of reducing, reusing and recycling (for minimizing pollution load) with

special reference to wastewater and solid wastes.

Resources

Recommended

Books

1. Bhojwani, S.S. and Razdan, M.K., (1996). Plant Tissue Culture: Theory

and Practice. Elsevier Science Amsterdam. The Netherlands.

2. Text Book of Biotechnology. 2004. H.K. Das (ed

New Delhi.

3. H. S. Chawla (2002). Introduction to plant biotechnology; Science

Publishers

4. Dubey, R. C. (1993

Reference

Books

1. Palmer, T. and Bonner, P. 2008: Enzymes

Clinical Chemistry (2nd Edition). East West Press Pvt. Ltd., New Delhi.

2. Johansson, T. B., & Burnham, L. (Eds.). (1993).

sources for fuels and electricity

understand the basic Biotechnology and its scope

understand Plant Tissue Culture techniques and their applications

understand Fermentation technology and its application.

understand bio fuels, their production and uses.

Solid waste management, physic-chemical characteristics, hazardous and

nonhazardous wastes, biodegradable and nonbiodegradable wastes, collection and

transport of solid wastes.

Disposal methods of solid wastes with special references to landfill, secured

landfill, biological compositing.

Concept of reducing, reusing and recycling (for minimizing pollution load) with

special reference to wastewater and solid wastes.

Bhojwani, S.S. and Razdan, M.K., (1996). Plant Tissue Culture: Theory

and Practice. Elsevier Science Amsterdam. The Netherlands.

Text Book of Biotechnology. 2004. H.K. Das (ed). Wiley India Pvt. Ltd.,

New Delhi.

H. S. Chawla (2002). Introduction to plant biotechnology; Science

Publishers

Dubey, R. C. (1993). A textbook of Biotechnology. S. Chand Publishing.

Palmer, T. and Bonner, P. 2008: Enzymes-Biochemistry,

Clinical Chemistry (2nd Edition). East West Press Pvt. Ltd., New Delhi.

Johansson, T. B., & Burnham, L. (Eds.). (1993). Renewable energy:

sources for fuels and electricity. Island press.

Pa

ge2

understand Plant Tissue Culture techniques and their applications

understand Fermentation technology and its application.

chemical characteristics, hazardous and

nonhazardous wastes, biodegradable and nonbiodegradable wastes, collection and

Disposal methods of solid wastes with special references to landfill, secured

Concept of reducing, reusing and recycling (for minimizing pollution load) with

Total 60 L

Bhojwani, S.S. and Razdan, M.K., (1996). Plant Tissue Culture: Theory

and Practice. Elsevier Science Amsterdam. The Netherlands.

). Wiley India Pvt. Ltd.,

H. S. Chawla (2002). Introduction to plant biotechnology; Science

. S. Chand Publishing.

Biochemistry, Biotechnology,

Clinical Chemistry (2nd Edition). East West Press Pvt. Ltd., New Delhi.

Renewable energy:

Year: Second

Course: Biotechnology Laboratory

Teaching

Scheme

(Hrs/Week)

Continuous Internal Assessment (CIA)

L T P C CIA-1 CIA-2

0 0 2 1 - -

Max. Time, End Semester Exam (

Objectives

1 To learn the Plant Tissue Culture Techniques

2 To understand fermentation process

3 To understand parameters of waste water.

Sr. No.

1 Demonstration of biotechnology laboratory and safety measures

2 MS medium composition, medium preparation and growth regulators

3 Callus induction from different explants

4 Preparation of synthetic seeds

5 Fermentation of citric acid and assay by titration

6 Visit to at least one fermentation industry/sewage water treatment plant.

7 Visit to a Plant Tissue Culture industry

Term Work:

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

continuous assessment based on

journal/record book, oral/viva, respectively

the end of the semester, the final grade for a Term Work shall be assigned based on the performance of

the student and is to be submitted to the University.

Notes

1 The regular attendance of the

marks will be given accordingly (

2 Good Laboratory Practices (

3 Timely Completion (05 Marks)

4 Journal / Record Book (05 Marks)

5 Oral / Viva (05 Marks)

Practical/Oral/Presentation:

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

appointed as internal and external examiners by the University. The examiners will prepare the

mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope

shall be submitted to the head of the department or authorized person.

Notes

1 One experiment from the regular practical syllabus will be conducted (

2 Oral/Viva-voce (05 Marks).

School of Science B.Sc. Microbiology

Semester: I

Laboratory Course Code:

Continuous Internal Assessment (CIA) End Semester

Examination

2 CIA-3 CIA-4 Lab Theory Lab

- - 25 - 25

End Semester Exam (Practical) – 2 Hrs.

To learn the Plant Tissue Culture Techniques

To understand fermentation process

To understand parameters of waste water.

Description

of biotechnology laboratory and safety measures.

MS medium composition, medium preparation and growth regulators

Callus induction from different explants

Preparation of synthetic seeds.

Fermentation of citric acid and assay by titration method.

Visit to at least one fermentation industry/sewage water treatment plant.

Visit to a Plant Tissue Culture industry.

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

continuous assessment based on attendance, good laboratory practice (GPL), timely completion,

journal/record book, oral/viva, respectively. It should be assessed by course teacher of the institute. At

the end of the semester, the final grade for a Term Work shall be assigned based on the performance of

the student and is to be submitted to the University.

the students during semester for practical course will be monitored and

rks will be given accordingly (05 Marks).

Good Laboratory Practices (05 Marks)

Marks)

Marks)

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

appointed as internal and external examiners by the University. The examiners will prepare the

as specified by the University, authenticate and seal it. Sealed envelope

shall be submitted to the head of the department or authorized person.

One experiment from the regular practical syllabus will be conducted (20 Marks).

Pa

ge1

Semester: III

Course Code: XBO311

Total

50

MS medium composition, medium preparation and growth regulators.

Visit to at least one fermentation industry/sewage water treatment plant.

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

attendance, good laboratory practice (GPL), timely completion,

teacher of the institute. At

the end of the semester, the final grade for a Term Work shall be assigned based on the performance of

practical course will be monitored and

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

appointed as internal and external examiners by the University. The examiners will prepare the

as specified by the University, authenticate and seal it. Sealed envelope

0 Marks).

Pa

ge2

School: Science Programme: Bachelor of Science (B.Sc.)

Year : Second Year Semester- III

Course: Organic and Inorganic Chemistry

II

Course Code: XCH301

Theory: 4 Hrs/Week Max. University Theory Examination:50

Marks

Max. Time for Theory Exam: 3 Hrs Continuous Internal Assessment: 50

Marks

Course Objectives

1 To learn a structure, properties, preparations and reactions of important organic

functional groups such as alkyl halide, alcohols, ethers, aldehydes and ketones

2 To learn a chemistry of s-block elements

3 To study a metallurgy and related terms.

Unit

Number Details Hours

1 Alkyl Halides, Alcohols and Ethers

Alkyl Halides: Introduction and classification of halogen derivatives,

structure of alkyl halides, physical properties, preparation, reactions,

analysis of alkyl halides.

Alcohols: Introduction, physical properties, reactions of alcohols,

industrial sources of ethyl alcohol, proof, spirit, denatured spirit, absolute

alcohol, analysis of alcohols.

Ethers: Introduction, physical properties, industrial sources, preparations,

Williamson’s synthesis, diazomethane method, reactions of ethers.

10

2 Aldehydes and Ketone, Carboxylic Acid and its Derivatives

Introduction and structure of carbonyl group,

Aldehydes- Preparation by reduction of acid chlorides, from Grignard’s

reagent and HCN, from terminal geminaldihalides, from calcium salt of

acids.

Ketones- Preparation from Grignard’s reagent and R-CN, from non

terminal germinal dihalides, from calcium salt of acids.

Reaction of aldehydes & Ketones- Reducing properties of aldehydes,

reaction with Tollen’s reagent and Fehling’s solution, Clemmenson

reduction, Wolff Kishner reduction, Aldol condensation, Cannizzaro

reaction, addition of HCN, NaHSO3, addition of derivatives of ammonia

10

(hydroxyl amine, phenyl hydrazine, 2,4 DNP, semicarbazide), haloform

reaction.

Carboxylic Acid and its Derivatives : Introduction and only structures

of Carboxylic Acid and its Derivatives

3 I. Benzene & its Reactions

Structure of benzene, Kekule structure, stability of benzene, aromaticity,

Huckel rule, reactions of benzene: sulphonation, halogenation, Friedal–

Crafts reactions of benzene.

II. Phenols

Structure, classification, physical properties, preparation of phenols,

industrial source, reactions of phenols: nitration, sulphonation,

halogenation, nitrosation, carbonation (Kolbe synthesis), Reimer–

Tiemann reaction & analysis of phenols.

10

4 I. Chemical Bonding and Structure

Attainment of stable configuration.

Types of bonds a) ionic; b) covalent; c) coordinate; d) metallic.

Types of overlap, formulation of σ and π bonds s–s overlap, p-p overlap,

p-d overlap with suitable examples, theories of bonding, valence bond

theory a) Hitler London theory and b) Pauling Slater theory.

II. Concept of hybridization

i) Definition, need of hybridization, steps involved in hybridization,

explanation of covalency of atoms in the molecules on the basis of

hybridization, types of hybridization involving s, p orbitals and s, p and d

orbitals, applications of hybridization concept to explain geometries of

molecules like BeF2, CH4, BF3, SiCl4, PCI5, IF7, SF6, [Ni (CN)4 ]2-

ii) VSEPR theory: Assumptions, need of theory, application of the theory

to explain geometry of irregular molecules like H2O, NH3, TiCl4, ClF3,

ICl2, BrF3, BrF5, OF2.

15

5 I. Chemistry of “s” Block Elements

Position of elements in periodic table, electronic configuration, periodic

trends in properties viz. size of atom, ion, oxidation state, ionization

potential, & reactivity, anomalous behavior of Li & Be, diagonal

relationship between Li & Mg, industrial, biological and Agricultural

applications of these elements & their compounds, crown ethers,

separation of these elements using crown ethers, solution of these metals

in liquor NH3

15

II. General Principles of Metallurgy:

Introduction, occurrence of metals, ores and minerals, types of ores,

operations involved in metallurgy, crushing, connotation, various methods

of concentration such as hand picking, gravity separation, magnetic

separation. Froth flotation, Calcinations, Roasting etc. reduction, various

methods of reduction such as smelting, Alumino thermic process and

electrolytic reduction, refining of metals, various methods of refining such

as poling, liquation, electrolytic and vapor phase refining (Van Arkel

Process).

Total 60

Course Outcomes

1 Student able to explain structure, properties, preparations and reactions of

important organic functional groups such as alkyl halide, alcohols, ethers,

aldehyde and ketones

2 Student able to understand and explain a chemistry of s-block elements

3 Student able to understand and explain principle, and process of metallurgy

Resources

Reference Books 1. Organic Chemistry by Clayden, Oxford uni.press.

2. Organic Chemistry by Morrison & Boyd, 6th Edition.

3. A guide book to Mechanism in Organic Chemistry by Peter

Sykes, 6th Edition.

4. Concise Inorganic Chemistry by J.D. Lee, Chapman & Hall 5th

Edn. (1996).

5. Concepts and Models of Inorganic Chemistry by B. Douglas &

D. Mc. Daniels Alexander Mohan Wiley & sons 3rd Edn. (2007).

6. Inorganic Chemistry Principles of structure & reactivity by

James Huheey, Keiter, Medhi (Pearson Education).

School: Science Programme: Bachelor of Science (B.Sc.)

Year: Second Year Semester - III

Course: Organic and Inorganic Chemistry

Laboratory II

Course Code: XCH311

Practical: UG - 2 Hrs./Batch (20 Students) Practical Examination: 25 Marks

Term Work: 25 Marks

Course Objectives

1 To learn an organic qualitative analysis

2 To learn an inorganic qualitative analysis

Sr.

No. Description

1 Organic Chemistry Practical

Organic qualitative analysis of Binary Mixtures (Minimum Four)

(Type determination, separation and physical constant)

Solid-solid, Solid-liquid, Liquid-liquid

2 Inorganic Chemistry Practical (Minimum Four)

i. One simple mixture (without phosphate or borate)

ii. Two Mixtures containing PO43- (With PO4

3- removal)

iii. Two Mixtures containing BO33- (With BO3

3- removal)

Term Work:

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work

is continuous assessment based on work done, submission of work in the form of

report/journal, timely completion, attendance, and understanding. It should be assessed by

subject teacher of the institute. At the end of the semester, the final grade for a Term Work

shall be assigned based on the performance of the student and is to be submitted to the

University.

Notes

1 The experiments from the regular practical syllabus will be performed (15 Marks).

2 The regular attendance of students during the syllabus practical course will be

monitored and marks will be given accordingly (5 Marks).

3 Good Laboratory Practices (5 Marks)

Practical/Oral/Presentation:

Practical/Oral/Presentation shall be conducted and assessed jointly by internal and external

examiners. The performance in the Practical/Oral/Presentation examination shall be assessed

by at least a pair of examiners appointed as examiners by the University. The examiners will

prepare the mark/grade sheet in the format as specified by the University, authenticate and

seal it. Sealed envelope shall be submitted to the head of the department or authorized person.

Course Outcomes

1 Student able to perform organic qualitative analysis

2 Student able to perform inorganic qualitative analysis

Notes

1 One experiment from the regular practical syllabus will be conducted. (Total 15

Marks).

2 Complete laboratory journal/records (05 Marks).

3 Viva-voce (05 Marks).