€¦ · web view30/04/2018 · able rwltig proposal 2018 s. catherine s. key, at nccu (hbcu)...

Summary:The rapid development of CRISPR technology as a tool for gene editing and scientific discovery has revolutionized the tools available for scientists in the research laboratory. However, there is a lack of laboratory modules available for undergraduate students to perform experiments employing CRIPR technology in the classroom. The laboratory module proposed here allows students to not only learn more about the theory behind CRISPR but also implement them in a hands-on manner. This lab module includes a theoretical section where students perform bioinformatics research to design the single-guide RNA they will later employ for the experimental section of the module. This laboratory requires several steps to be completed by the professor outside of the classroom in order to have students in the laboratory perform the key steps in the protocol. Bacterial gene knockdown using CRISPRi is a simple way to introduce students to CRISPR technology as well as basic microbiology techniques and protocols.

Detailed Outline of the Proposed ProjectTopic:Implementation of CRISPRi in the classroom: Lab module for bacterial gene knockdown using CRISPRi technology

Purpose:Introduce students to CRISPR and CRISPRi technology. They will learn how to use bioinformatic tools to generate the materials necessary for the experiments to implement CRISPRi and will be able to perform several steps of the protocols in-lab.

Description:In the theoretical component of this lab students will learn the basics of CRISPR technology and its applications in bacterial systems. They also will implement bioinformatic tools to design their own single-guide RNA (sg-RNA) for the lacZ gene in bacteria as well as the primers necessary for inverse PCR (iPCR). In the hands-on component of this lab, students will perform the transformation steps necessary to effectively perform CRISPRi and knockdown the expression of lacZ in a laboratory strain of E. coli.

Theoretical component of laboratory module: *Pre-lab handout Introduction to Bacterial Gene Editing with CRISPR technology Introduction to CRSPRi technology and sgRNA design Design of sgRNA and completion of in-lab handout Complete components of the Wet-lab

o Inquiry-based approach: students will not be told what to expect; rather, the possible ways in which CRISPRi can be used will be discussed.

o sgRNA-containing reagents and water will be labeled 'Reagent-A' and 'Reagent-B'o Students will need to analyze data and discuss with peers the results to come to a

conclusion about which sample contained the sgRNAs and explain how they arrived at that conclusion.

o Easily observable colorimetric results on petri dish: How will they know: in this exercise, the if CRISPRi activity targets the lacZ gene effectively, then the mutated gene will make a non-functional beta-galactosidase enzyme. Thus, X-Gal will not be converted to blue product.

Turn in laboratory report which will include application of the learned procedure to a Genetic's topic of interest: deletion of overactive gene, repair of defective gene, etc related to some human disease: preferably one associated with Health Disparity.

*Post-test and post-module survey collected* once funding is approved, IRB will be submitted at NCCU; it is expected that the analysis of pre-/post-quiz and post-module survey will be exempted. Dr. Key is currently CITI Certified for working with Human Subjects.

Table 1. Experimental components of laboratory module:

ABLE 2018 RWLTIG: CRISPRi-Targeting the E. coli lacZ Gene S.C.S. Key, at NC Central University (an HBCU) is P.I., N. Orench-Rivera, grad student at Duke

in Preparing Future Faculty program is co-P.I.

ABLE RWLTIG Proposal 2018 S. Catherine S. Key, at NCCU (HBCU) P.I.

Protocol To be performed…A. Design of sgRNA primers using online Bioinformatic tools:

Week prior to this module, student will complete Pre-lab CRISPR & CRISPRi assessment quiz

Pre-lab: ncbi.nlm.nih.gov ecogene.org

In-lab: chopchop.cbu.uib.no rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi

Module Day 1: sgRNA designBY STUDENTS in lab

B. Inverse PCR Phosphorylation of primers Amplification of sgRNA vectors

o Perform iPCRo Purify PCR productso Dpn treatmento Run gel and staino Purify from gelo Circularize

Miniprep DNA from cells with dCas9 vector

OUTSIDE OF LAB PERIOD

C. Transformation Transformation and incubation overnight

Module Day 2: Analyze and Discuss iPCR Results &

TransformationBY STUDENTS in lab

D. Grow transformed cells Inoculate colonies into media and incubate overnight


E. Preparation of plasmids Pellet cells and miniprep DNA Co-transform with vector from dCas9-expressing

vector

Module Day 3: Plasmid Prep & Co-transformation with sgRNA

and Cas9 enzymeBY STUDENTS in lab

F. Re-inoculation in LB with Amp and Cm, no inducers Grow overnight


G. Dilute colonies and grow them for 6 hours with inducer Plate on selective agar to test for phenotype Parafilm them the next day


H. Check plates for phenotype. Complete post-lab handout Complete post-lab assessments: quiz & survey

Module Day 4: Analyze & Discuss RNAi Results

BY STUDENTS OUTSIDE OF LAB PERIOD

Additional materials that need to be prepared: Selective Agar Media with antibiotics

The following are already available and/or will be provided by the Department of Biological & Biomedical Sciences at NCCU:

Incubator with shaker Centrifuge Pipettes and tips Heat block

Student work assessment criteria:

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Students will complete a pre-assessment quiz and then a pre-lab handout, answering questions regarding the theory behind CRISPR and CRISPRi and the generation of the single-guide RNA prior to class. Subsequently, students will complete the 1 in silico lab and 3 wet-lab components indicated in Table 1., and then complete post-quiz and survey. Questions will be Multiple Choice (MC).

Example pre-/post-assessment questions:1. MC. The CRISPR-Cas9 occurs naturally in which of the following organisms:

a. Eubacteriab. Yeastc. Protistsd. Multicellular organismse. All of the above

2. MC. Which of the following RNA molecules is used to target DNA in CRISPR-Cas9 system?a. sgRNAb. microRNAc. siRNAd. tRNAe. rRNA

3. Which part of the below picture is considered the Cas9 handle?a. Structure Ab. Structure Bc. Structure Cd. Structure D

In class, students will design the sg-RNA and primers needed for iPCR with the use of bioinformatic tools such as NCBI databases and sg-RNA and RNA design websites. They will complete an in-lab handout documenting their progress designing the sg-RNA for the target gene.Students will perform two bacterial transformations as part of the CRISPRi protocol. They will be evaluated on sterile lab techniques. After completion of the experimental component of this lab, students will be asked to complete a post-lab handout documenting and discussing critically the results of the experiment.

Reference: Hawkins JS, Wong S, Peters JM, Almeida R, Qi LS. Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi). Methods Mol Biol. 2015;1311:349-62.

BUDGET:

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Table 2. Materials with estimated costs for budget:

Product Details/Vendor PriceE. coli sgRNA expressin plasmid with ampicillin resistance

sgRNA is expressed from a strong constitutive promoter J23119. Addgene

$65.00

Designed forward sgRNA primers

Eurofins genomics $15.00

Universal reverse primer Eurofins genomics $15.0010 mM ATP New England BioLabs $33.00T4 Polynucleotide Kinase Kit

New England BioLabs $56.00

2x Phusion PCR Master Mix New England BioLabs $175.00QIAQuick PCR Purification Kit

Qiagen $115.00

DpnI Kit Qiagen $63.00QIAQuick Gel Extraction Kit Qiagen $116.00Quick Ligase Kit New England BioLabs $97.00One Shot TOP10 chemically competent E. coli cells

Thermofisher $248.00

LB Agar (powder, Lennox L agar)

Thermofisher scientific $112

Petri dishes Fisher scientific $217.80Ampicillin Sigma $68.30TYGPN broth Amresco $76.50Breathable Sealing Films Sigma $51.20MiniPrep Kit Qiagen $ 92.80X-gal solution ready-to-use $ 120Chloramphenicol Sigma $ 33.70Anhydrotetracycline Cayman Chemical $25.00E. coli dCas9 expression plasmid with chloramphenicol resistance

Addgene ID no. 44249 $65.00

Eppendorf tubes Thermofisher scientific $73.00Plastic culture tubes Fisher scientific $223.8796-well, 2mL deep culture plates

USA scientific $87.55

Parafilm Sigma Aldrich $100.70Shipping and Handling costs

$200.00

REAGENT TOTAL: $2,548.00

Budget Justification: The requested supplies are necessary to develop the laboratory for one section a Biology Major's required Genetics course of 24 students.

Sustainability of Project post-ABLE funding: Since the CRISPRi lab is being developed for a required Biology majors course, Genetics (BIOL3100), Departmental funds are available to support the CRISPRi module once it has been developed. Per semester, Genetics receives approximately $2000 to purchase items for the teaching laboratory (please see chairperson, Greg Cole’s, Letter of Support). Once the plasmids, primers, antibiotics and kits are purchased and the system is up and going, we can move away from expensive kits to execute the wet-lab. The aim is for the students to have a ‘hands-on’ experience,

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but to have a T.A. (also provided by the Department) to perform a number of steps outside of the classroom; thus, reducing expense while still providing a ‘hands-on’ component (2 of the 3 planned CRISPRi modules) to students enrolled in Genetics. Thus, once the process of discovering undergraduate student deliverables has been worked out using funds from the Roberta Williams Laboratory Teaching Initiative Grant, purchasing reagents to sustain the deliverables to future students can be accomplished out of the Departmental budget.

Safety Standards and Animal Use Protocols:

No animals will be used in this study and therefore, the animal use protocols are not applicable. Safety standards: Students will wear protective eye-wear, gloves and lab coat to perform wet-lab

steps and dispose of tips and other materials in the Biohazard waste receptacles that will be provided. All biohazard material will be autoclaved as per NCCU Health & Safety standards.

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Curriculum VitaeS. Catherine Silver Key

Contact: Department of Biological and Biomedical SciencesNorth Carolina Central University,2246 Mary Townes New Science Center, Durham, NC 27707Email: [email protected] Phone: 919/530-5365 Fax: 919/530-7773

Education:

2012-current Associate Professor of Biology at North Carolina Central University (NCCU

2005-2012 Assistant Professor of Biology at North Carolina Central University (NCCU)

2001-2005 SPIRE (Seeding Postdoctoral Innovators in Research and Education; http://spire.isl.unc.edu/ ) Postdoctoral Fellow, Biology: Molecular and Developmental Genetics, University of North Carolina at Chapel Hill

1998 Postdoctoral Research Assistant, Biochemistry, Duke University

1997 Ph.D., Microbiology and Immunology, University of North Carolina at Chapel Hill.

1988 B.S. “Magna Cum Laude”, Biology, University of Missouri at St. Louis.

Professional Organization Memberships:

2016-current NC State KECK Center Neuroscience visiting member

2010-2014 North Carolina Association for Science (NCAS)

2010-current Genetics Society of America (GSA)

2009-2012 Society for Developmental Biology (SDB)

2009-current Genome Education Partnership (GEP) [Dr. Elgin, Washington Univ.]

2007-current Association for Biological Laboratory Education (ABLE).

2007 Council for Undergraduate Research (CUR)

August, 2006 Inducted into local Chapter of Sigma Xi Research Society

Teaching Experience:

2012-current Associate Professor of Biological and Biomedical Sciences at NCCU courses:Beginning F2014 Advanced Integrated Genetics (BIOD8020)Genetics (BIOL3100) each semester plus some summersDevelopmental Biology BIOL4100Introduction to Research (BIOL4400), Research in Biology (BIOG5800)Occasionally teaching Molecular Biology of the Cell (BIOL2200)Beginning S2015 Assisting with Senior Seminar BIOL4040 Capstone Experience

2005-2012 Assistant Professor of Biology at NCCU teaching:F2013-current Graduate Genetics (BIOG5120)F20011 & F2012-current Molecular Biology of the Cell (BIOL2200)F2010, F2012, S2013 Thesis (BIOG5900)

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http://spire.isl.unc.edu/

mailto:[email protected]


F2009-2011 Graduate Research Rotations (BIOG5820)S2008-current Research in Biology (BIOG5800)2007, 2010-current Spring Inquiries in Developmental Biology (BIOL4100)2005-2006 General Biology II for majors (BIOL1201)2005-2009 Fall only; Techniques in Biochemistry and Molecular Biology (BIOL4550)

2005-current Genetics (BIOL3100) each semester2006-current Introduction to Research (BIOL4400)

2004-2005 SPIRE fellow guest lecturer at: Johnson C. Smith University/Fayetteville State University (videoteleconference), Durham School of Science and Math, North Carolina State University Biotechnology Education Facility

(www.ncsu.edu/biotechnology), and DESTINY (http://moreheadplanetarium.org) traveling high school science laboratory.

2003-2004 Visiting Assistant Professor of Biology, North Carolina A&T State University Teaching Molecular Genetics (F2003) and Cancer Biology (S2004)

1999-2001 Adjunct Assistant Professor of Biology, Elon University teaching: Cell Biology, Molecular Cell Biology, and General Biology for non-majors.

1990-1991 Graduate Teaching Assistant of Microbiology, UNC at Chapel Hill

Research Positions:

2012-current Associate Professor of Biological and Biomedical Sciences at NCCU

2005-2012 Assistant Professor of Biology at NCCU, collaborating with Dr. Robert Duronio on l(2)dtl/cdt2 role during Drosophila development at UNC-CH SCORE SC2 NIH/NIGMS grant awarded July 1, 2008-extended 2012.Separate project is the Genomics Education Partnership, P.I. Sarah Elgin – collaborative science education and scientific research focused on annotation of Drosophila genes in multiple species. Third project is collaboration with Dr. John Bang at NCCU on the effect of silver nanoparticles on Drosophila development. Other scientific education projects: ABLE 2012-2013 grant for introducing a wet-lab to study regulation of Drosophila gene regulation and inheritance research experience for non-majors in English writing course.

2002-2005 Postdoctoral Research Assistant in Seeding Postdoctoral Innovators in Research and Education (SPIRE) Program, Department of Biology, University of North Carolina at Chapel Hill with Drs. Robert J. Duronio and Leslie Lerea.

1997- 1998 Postdoctoral Research Assistant, Department of Biochemistry, Duke University with Dr. Arno Greenleaf.

1993-1997 Graduate & Postdoctoral Research Assistant, Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, University of North

Carolina at Chapel Hill with Dr. Joseph S. Pagano (Epstein-Barr Virus)

1989-1993 Graduate Research Assistant, Department of Rheumatology and Immunology, University of North Carolina at Chapel Hill with Dr. S. Hunt (Stem Cell Leukemia gene)

1988-1989 Research Assistant, Center for Genetics and Medicine, Washington University, St. Louis with B. Brownstein (Human Genome Project)

1987-1988 Undergraduate Research Assistant, University of Missouri at St. Louis

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http://moreheadplanetarium.org/


Course Redesigns:2005-Current Redesigned Inquiries in Developmental Biology (BIOL4100) S2017 Molecular Biology of the Cell (BIOL200) Course Research Experience re-design [2013-2014] Genetics (BIOL3100) lecture & lab – Lecture updated at least every 2 years with new editions of

the textbook. Lab updated with Genomics Research in classroom (2009), Drosophila lacZ modules (2011), “Learning the Genes” Bioinformatics modules (2016), Alcohol Behavior Assays on Drosophila (2018)

General Biology II (BIOL1201) lecture & lab [2005-2006] Techniques in Biochemistry and Molecular Biology (BIOL/CHEM4550) lab [2005-2008] Reactivated ‘Inquires in Developmental Biology” (BIOL4100) S2007-current.

Trained and continued to mentor research students in non-didactic courses: Introduction to Research (BIOL4400) – 1-5 undergraduates per semester research S2006-current Graduate Research Rotations course (BIOG5820) 1-2 grad/sem. Research in Biology (BIOG5800) – 1-2 grad/semester S2008-current Thesis (BIOG5900) – Roketa Sloan, M.S. Fall 2010

Denise Reaves, M.S. Fall 2012Kristin Smith Kemp, M.S. S2013Maryonne Snow-Smith, M.S. S2013Chelsea Fulmore, M.S. F2016

S2017 Redesign BIOL4100 Inquiries in Developmental Biology. Seven Bioethical discussions and completely rearranged 11th Edition of Developmental Biology textbook by Scott Gilbert and new author Michael Barrisi prompted complete revamping of all lecture material.

2003-2004 Designed and taught upper level undergraduate/Graduate courses: ‘BIOL630: Molecular Genetics’ with laboratory and ‘BIOL642: Special Topics in Biology: Cancer Biology’ at North Carolina A&T State University

F1999-F2001 Designed and taught the following: ‘BIOL101: Topics in ‘General Biology’, Laboratory, ‘Current Issues in Biology’, ‘Introductory Cell Biology’, and ‘Cell and Molecular Biology’. Also taught the following: ‘BIOL102: General Bi-ology and ‘Cell Biology Laboratory’ at Elon University.

1990-1991 Taught ‘Microbiology Laboratory’ at UNC at Chapel Hill

Peer-reviewed Research Journal Publications:

Leung, W, … [S.C. Silver Key, B.D. McRae] …S.C.R. Elgin [239 student co-authors, 40 faculty co-authors]. 2017, G3: Gene, Genomes, Genetics. 7(8): 2439-2460. Retrotransposons Are the Major Contributors to the Expansion of the Drosophila ananassae Muller F Element. Note: I mentored Briana McRae. She was an undergrad who annotated two 40 kB contigs during her 1-year BIOL4400 research experience. She is now in Howard University Dental School

Leung, W, ...[ Tyneshia C. P. Henry, Ashlee G. Johnson, Jackie X. White, S. Catherine Silver Key], ...Elgin, SCR (2015) "The Drosophila Muller F elements maintain a distinct set of genomic propertiesover 40 million years of evolution." G3: GENES, GENOMEs,GENETICS. 5(5): 719-40. [Total 940 student c0-authors, 74 faculty co-authors; TCPH, AGJ and JXW were NCCU undergraduates when they completed their work for the publication.]

Roketa S. Sloan, Christina I. Swanson, Lily Gavilano, Kristen N. Smith, Pamela Y. Malek, Maryonne

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Snow-Smith, Robert J. Duronio, and S. Catherine Silver Key. 2012. Characterization of null and hypomorphic alleles of the Drosophila l(2)dtl/cdt2 gene: larval lethality and male fertility, Fly 6 (3): 173-183.

Key, S.C.S., Reaves, D.K., Turner, F., and J.J. Bang. 2011. Impacts of Silver Nanoparticle Ingestion on Pigmentation and Developmental Progresseion in Drosophila. Atlas Journal of Biology, 1(3): 52-61.

Cayirlioglu, P., W. O. Ward, S.C.S. Key, and R. J. Duronio. 2003. Transcriptional repressor functionsof Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. Mol. Cell. Biol. 23(6): 2123-2134.

Key, S.C.S., Yoshizaki, T. and J.S. Pagano. 1998. The Epstein-Barr Virus (EBV) SM Protein Enhances Pre-mRNA Processing of the EBV DNA Polymerase Transcript. J. Virol. 72(11): 8485-

8492.

Key, S.C.S. and J.S. Pagano. 1997. A Noncanonical Poly(A) Signal, UAUAAA, and Flanking Elements in Epstein-Barr Virus DNA Polymerase mRNA Function In Vitro. Virology. 234: 147-159.

Silver, S.C. and S.W. Hunt, III. 1993. Techniques for Cloning cDNAs Encoding Interactive Transcriptional Regulatory Proteins. Mol. Biol. Rep . 17: 155-165.

Peer-reviewed Scientific Education Journal Publication:

Laakso, MM, LV Paliulis, P Croonquist, B Derr, E Gracheva, C Hauser, C Howwel, CJ Jones, JD Kagey, J Kennell, SC Silver Key, H Mistry, S Robic, JSanford, M Sanisteban, C Small, R Spokony, J Stamm, M Van Stry, W Leung, SCR Elgin. 2017, CourseSource. An undergraduate bioinformatics curriculum that teaches eukaryotic gene structure. https://doi.org/10.24918/cs.2017.13

*Notes: I wrote Module 3 (of 6 modules); participated in designing pre-/post- assessments, gathered student data and collaborated with 2 co-authors to design the Power Point mini-lesson.

David A. McDonald*1, Sarah E. Council*1, Stephanie C. Schroeder2, Ruth S. Phillips* 3, Gail P. Hollowell#1,3, and S. Catherine Silver Key#*3 * These authors contributed equally and should be considered *co-first authors; # co-senior authors. 2016 “Identifying Promoter Activators and Repressors Using lacZ Transgene Expression in Saccharomyces cerevisiae”. Tested Studies for Laboratory Teaching: Proceedings of the Association for Biology Laboratory Education, Volume 37, Article 13, 2016.

Lopatto D, Hauser C, Jones CJ, Paetkau D, Chandrasekaran V, Dunbar D, MacKinnon C, Stamm J, Alvarez C, Barnard D, Bedard JE, Bednarski AE, Bhalla S, Braverman JM, Burg M, Chung HM, DeJong RJ, DiAngelo JR, Du C, Eckdahl TT, Emerson J, Frary A, Frohlich D, Goodman AL, Gosser Y, Govind S, Haberman A, Hark AT, Hoogewerf A, Johnson D, Kadlec L, Kaehler M, Key SC, Kokan NP, Kopp OR, Kuleck GA, Lopilato J, Martinez-Cruzado JC, McNeil G, Mel S, Nagengast A, Overvoorde PJ, Parrish S, Preuss ML, Reed LD, Regisford EG, Revie D, Robic S, Roecklien-Canfield JA, Rosenwald AG, Rubin MR, Saville K, Schroeder S, Sharif KA, Shaw M, Skuse G, Smith CD, Smith M, Smith ST, Spana EP, Spratt M, Sreenivasan A, Thompson JS, Wawersik M, Wolyniak MJ, Youngblom J, Zhou L, Buhler J, Mardis E, Leung W, Shaffer CD, Threlfall J, Elgin SC. “A Central Support System Can Facilitate Implementation and Sustainability of a Classroom-Based Undergraduate Research Experience (CURE) in Genomics.” CBE Life Sci Educ. 2014 Winter; 13(4):711-23. doi: 10.1187/cbe.13-10-0200.bPMID: 25452493

S. Catherine Silver Key1, Julie Gates2, Jessica K. Sawyer3 and Kirsten A. Guss4 1 North Carolina Central University, 2 Bucknell University, 3 Duke University and 4 Dickinson College.

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https://doi.org/10.24918/cs.2017.13


2015. “Gene Expression Patterns in Drosophila Embryos Using lacZ Transgenes”. Tested Studies for Laboratory Teaching: Proceedings of the Association for Biology Laboratory Education, 36: 15-35, 2015.

Shaffer, C.D., C.J. Alverez, A.E. Bednarksi, D. Dunbar, A.L. Goodman, C. Reindke, A.G. Rosenwald, M.J. Woyniak, C. Bailey, D. Barnard, C. Bazinet, D.L. Beach, J.E.J. Bedard, S. Bhalla, J. Braverman, M. Burg, V. Chandrasekran, H.-M. Chung, K. Clase, R.J. DeJong, J.R. DiAngelo, C. Du, T.T. Eckdahl, H. Eisler, J.A. Emerson, A. Frary, D. Frohlick, Y. Gosser, S. Govind, A. Haverman, A.T.Hark, C. Hauser, A. Hoogewerf, L.L. M. Hoopes, C.E. Howell, D. Johnson, C. J. Jones, L. Kadlec, M. Kaehler, S. Catherine Silver Key, A. Klenschmit, N.P. Kokan, O. Kopp, G. Kuleck, J. Leatherman, J. Lopilato, C. MacKinnon, J. C. Martinez-Cruzado, G. McNeil, S. Mel, H. Mistry, A. Nagengast, P. Overvoorde, D. W. Paetkau, S. Parrish, C. N. Peterson, M. Preuss, L. K. Reed, D. Revie, S. Robic, J.Roecklein-Canfield, M.R. Rubin, K. Saville, S. Schroeder, K. Sharif, M. Shaw, G. Skuse, C.D. Smith, M.A. Smith, S. T. Smith, E. Spana, M. Spratt, A. Sreenivasan, J. Stamm, P. Szauter, J.S. Thompson, M. Wawersik, J. Youngblom, L. Zhou, E. R. Mardis, J. Buhler, W. Leung, D. Lopatto, and S.C.R. Elgin. 2014. “A Course-Based Research Experience: How Benefits Change with Increased Investment in Instructional Time.” CBE Life Sci Educ. 13(1): 111-30.

Julia A. Emerson, S. Catherine Silver Key, Consuelo Alvarez, Satish C. Bhalla, Stephanie Mel, Mary A. Smith, Gerard McNeil and Ken Saville. 2012. “ Introduction to the Genomics Education Partnership and Collaborative Genomics Research in Drosophila” ABLE Proceedings 34:135-165.

Hutchins, G., and S. C. Silver Key. 2008. “Gene Knockout/Gene Therapy in Yeast Using Homologous Recombination”. Peer-reviewed journal, ABLE Proceedings, 30: 49-62.http://www.ableweb.org/proc/contents.htm

Other Peer-reviewed Scholarly Science Education Submissions:

S.C.S. Key. December 2006. "Tazswana’s Story: A Case of How Alternative pre-mRNA Splicing Leads to Genetic Disease and Cure." National Center for Case Study Teaching in Science NSF, National Digital Library (NSDL) publication: http://www.sciencecases.org/tazswana/tazswana.asp University at Buffalo, State University of New York.

SCS Key 2011 ‘Don’t Drink and Fly” for prestigious AAAS Science Magazine website http://modencode.scimagdev.org/drosophila/alcoholism/ to introduce modENCODE interactive bioinformatics database . The vignette is one page of a 20 page national collaboration effort with Dr. Sarah Elgin and the international Genomics Education Partnership (GEP) consortium.

Guest Lectures:July 20 & 27, Two 2-hour workshops on “Intoxicated Flies and Statistics” at NC Museum of Natural 2017 Sciences. Audience: Middle-school aged Home schooled students.

Dec 3, 2012 Southern Alamance High School, Graham, NC “Model Organisms in Research and the pathway to Professor-hood” Host: Brenda Underhill. Spoke to 60 students in two, 1 hour sessions.

April 8, 2011 Southern Middle School in Alamance County: “Developmental Genetics Research Lab” presented to ~174 students in 20-40 min intervals for 4 hrs. Host: Katherine Gasper

Oct. 20, 2010 North Carolina School of Science and Math Research Presentation “Two research projects: 1. Nanoparticle effects on whole organisms 2. Gene called L2DTL important for cell cycle”. Host: Steve Warshaw

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http://modencode.scimagdev.org/drosophila/alcoholism/

http://www.sciencecases.org/tazswana/tazswana.asp

http://www.ableweb.org/proc/contents.htm


March 3, 2008 Jackie Swanik’s Inquiries in Developmental Biology course at NCCU: “Genetics of Axis Specification in Drosophila”

April 11, 2007 Guest Researcher Presentation to NCCU Senior Seminar Course (BIOL5750) taught by Dr. Sandra White.“The Role of L2DTL in Cell Cycle Control During Drosophila Development.”

April 6, 2006 Cancer Researcher Guest Lecture to NCCU Dr. Gail Hollowell’s Biology: “Retinoblastoma and the Role of the E2F Transcription Family in Cell Cycle Control During Drosophila Development”

Feb 10, 2006 Visiting Scientists Lecture at Durham NC School of Science and Math, Christine Muth’s Molecular Genetics Course: “Retinoblastoma”

Nov. 11, 2006 Guest Researcher Presentation to NCCU Dr. Sandra White’s Senior Seminar class: “The Role of the E2F Transcription Family in Cell Cycle Control during Drosophila Development”

Nov. 2005 Guest Researcher Presentation to NCCU Senior Seminar Course (BIOL5750) taught by Dr. Sandra White.“The Role of L2DTL in Cell Cycle Control During Drosophila Development.”

March 15, Video-teleconference Guest Lecture between Johnson C. Smith University and2005Fayetteville State University. Dr. Dinitra White’s Microbial Pathogenesis course: “Epstein-

Barr Virus: Causative Agent of Mononucleosis and Burkitt’s Lymphoma”2005

March 1, Host undergraduates from Historically Minority Universities on Research 2005Lab Tours, UNC at Chapel Hill. Introduced students to fly genetics in

laboratory setting and lectured on how we use flies as a model system to study human diseases.

January Visiting Scientists Lecture at Durham NC School of Science and Math, 18, 2005 Christine Muth’s Molecular Genetics Course: “How Animal Models

Help Us Understand Cancer”.

April 8, 2011 Cathy Silver Key delivered oral presentation of research to ~174 middle school students at Southern Alamance Middle School. Title: Developmental Genetics Research Lab

December Outreach lecture and lab tour “Cell Cycle Regulation During Drosophila 14, 2004 Development” DESTINY Caswell County High School Students at UNC-CH.

November 8 Guest lectures at North Carolina State University Biotechnology & 15, 2004 Education Facility. ‘Yeast Gene Knockout’ Lecture and Lab to undergraduates and

graduates enrolled in Mutagenesis course. Course instructor: Dr. Lisa K. Lyford.

April 1, 2003 Guest lecture on ‘Alternative Herbal Medicine’ Molecular Mechanisms of Drug Therapy at North Carolina Central University and Video- teleconferenced to UNC at Pembroke.

January 2003 Guest lecture on “Francis Collins and the Human Genome Project” in Dr. B. Knight’s Microbiology Nursing course at North Carolina A&T State University.

Oral Presentations International or Local Conferences by Invitation:

2017 SPIRE Grant-writing panel speaker, UNC-Chapel Hill, Fall 2017.

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2017 “Learning the Gene: Implementation of Bioinformatic Modules at NCCU” July 2017 at Genomics Education Partnership, Washington University at St. Louis.

2015 Sarah Council and S. Catherine Silver Key: two workshops on promoter-lacZ in yeast at 37th ABLE Conference, University of Boston, Boston, MA. Co-presenters.

2014 S. Catherine Silver Key: two workshops on enhancer-lacZ expression in Drosophila embryos at 36th ABLE Conference, University of Oregon, Eugene, OR.

June 20, Emerson, J.A.; Silver Key, S.C.S.; Alvarez, C.J., Mel, S.; McNeil, G.; Saville, K.J.; 2012 Leung, W., Shaffer, C.D., and S.C.R. Elgin 34th Annual Association for Biology Laboratory Education (ABLE) Major Laboratory Workshop on “The Genomics Education Partnership (GEP): undergraduate Student Analysis of Drosophila

Genomes”, Chapel Hill, NC. Dr. Key co-presented one of two sessions.

March 26, 2011. S. Catherine Silver Key 1 and Roketa Sloan2 The role of Cdt2 in Drosophila development Central University1 and Duke University2, Faculty

Oral Presentation at North Carolina Academy of Sciences, Elon University, NC

June 5, 2008 G. Hutchins and S.C. Silver Key Association for Biology Laboratory Education (ABLE) Major Laboratory Workshop on “Yeast Gene Deletion and Gene Therapy”, University of Toronto at Missassauga, Canada. Dr. Key co-presented both 3 hr sessions.

October Research Seminar at Meredith College “What microarrays on E2F mutant fly 28, 2004 embryos tell us about genes that regulate the cell cycle and genes that don’t!” and “Undergraduate Summer Research Opportunities” to Cell Biology students.

January Key, S.C.S. ‘SPIRE Fellow Meets the Fly: The Role of E2F2 During Drosophila2002 Embryogenesis.’ IRACDA (Institutional Research and Academic Career Development Award) National Conference. University of North Carolina at Chapel Hill.

September Key, C.S., Furnari, G. and J.S. Pagano. ‘Novel 3’ Processing of EBV DNA Polymerase1994 mRNA.’ Sixth International Symposium on Epstein-Barr Virus and Associated Diseases: Cancer Cells. Cold Spring Harbor, NY.

National Poster Presentations:

L. Pauliulis, Laakso, M., Santistban, Silver Key, S.C., M., Burgess, R., Sanford, J., Rosenwald, A., Bazinet, C. Van Stry, M., Leung, W. and SRC Elgin. A bioinformatics curriculum that teaches gene structure is associated with learning gains in beginning college students. American Society for Cell Biology (ASCB), Philadelphia, PA, December 3, 2017, Poster P1028 on Sunday 1-3 p.m.

SCS Key, R Sloan, C Swanson, M Snow-Smith, K Smith.’ Mutation of the lethal(2)denticleless gene results in larval lethality and sterility’. Presented at 54th Annual Drosophila Research Conference: Drosophila Genetics, March 7-11, 2012 Poster 293B, Dr. Key presented all three sessions: THU 3:00PM-4:00PM; FRI 9:00PM-10:00PM, and SAT 1:30PM-2:30PM in Chicago, IL.

SRC Elgin, W. Barshop, H. Yuan, M. Burg, C Coyle-Thompson, J DiAngelo, D. Johnson, C. Jones, L. Kadlec, SC Silver Key, NP Kokan, G McNeil, A Nagengast, DW Paetkau, KK Saville, S Smith, J Stamm, M Wawersik, L Zhou, D Lopatto. The Genomics Education Partnership (GEP): Comparative Analysis of the Drosophila Dot Chromosome by Undergraduate Students at 54th Annual Drosophila Research Conference: Drosophila Genetics, March 7-11, 2012 Poster 889A, Dr. Key presented one of the three sessions: FRI 10:00PM-11:00PM, in Chicago, IL.

DK Reaves, JJ Bang, SC Silver Key.’ In Vivo Exposure Impacts of Nano Silver on Drosophila melanogaster’ at 54th

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Annual Drosophila Research Conference: Drosophila Genetics, March 7-11, 2012 Poster 440B, Denise presented two of the three sessions: THU 2:00PM-3:00PM, FRI 9:00PM-10:00PM, in Chicago, IL.

SCR Elgin1, M Burg2, V Chandrasekaran3, HM Chung4, D Dorer5, D Johnson6, CJ Jones7, L Kadlec8, SC Silver Key 9 , G McNeil10, A Nagengast11, DW Paetkau12, S Parrish13, S Smith14, J Stamm15, M Wawersik16, L Zhou17, W Leung1, C Shaffer1, D Lopatto18 1Washington Univ-St. Louis MO, 2Grand Valley St Univ MI, 3St Mary’s Coll CA, 4Univ West Florida FL, 5Hartwick College NY, 6George Washington Univ, Washington DC, 7Moravian Coll PA, 8Wilkes Univ PA, 9NC Central Univ, NC, 10York Coll CUNY, NY, 11Widener Univ PA, 12St. Mary’s Coll IN, 13McDaniel Coll MD, 14Arcadia Univ PA, 15Univ Evansville IN, 16Coll William & Mary VA, 17Univ Pittsburgh PA, 18Grinnell Coll IA “The Genomics Education Partnership: Bringing Genomics into the Undergraduate Curriculum in Diverse Set” Poster 843C, 51st Drosophila Conference, Washington, D.C, April 7-11, 2010. Dr. Key presented one of 3 sessions.

Sloan, R. and S.C.S. Key. ‘The Function of l(2)dtl in Drosophila melanogaster’, 2008 Annual Biomedical Research Conference for Minority Students (ABRMCS), Orlando, FL. November 8, 2008.

Mollison, L.* and S.C.S. Key. ‘Understanding the role of the E2F responsive gene, CG11295 [l(2)dtl], in the G1/S checkpoint during Drosophila melanogaster development.’ 2006 FASEB Summer Research Conferences: Transcriptional Regulation during Cell Growth, Differentiation, and Development. Saxtons River, VT. August 12-17, 2006 also presented at Annual Biomedical Research Conference for Undergraduates, San Diego, CA Nov 8-11, 2006 (as MARC scholar).* Undergraduate in Partnership for Minority Advancement in Biomedical Sciences (PMABS) program at NCCU

Key, S.C.S., Swanhart, L.M., and R. J. Duronio. ‘Cellular localization of E2F and RB family Members during Drosophila development.’ 4th Annual IRACDA Meeting, Emory University, Atlanta, GA, June 2004.

Key, S.C.S. and G. G. Hutchins. Down for the Ade2 Count and …KNOCKOUT! A Laboratory Learning Module to Visualize Homologous Recombination and Gene Knockouts.’ 4th Annual IRACDA Meeting, Emory University, Atlanta, GA, June 2004.

Key, S.C.S. Cayirlioglu, P., W. O. Ward, and R. J. Duronio. ‘Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells.’ 44th Annual Drosophila Conference, March 5-9, 2003.

Lerea, L. S. , A. T. Baines, G. G. Hutchins, M. McVey, S. C. S. Key, C. D. White, K. L. Williams, and W. E. Bollenbacher. ‘SPIRE – An Innovative Model of Postdoctoral Training Enhances Science Education at Historically Minority Universities. ‘AAAS Annual Meeting, February 13-18, 2003.

Student Oral Presentation, Local Meeting:

Chelsea Fulmore (Silver Key lab) “Investigation of Cdt2 and male sterility in Drosophila” 8th Annual NCCU College of Science and Technology Research Symposium April, 2016. Note: Ms. Fulmore won 3rd place award ($50) for oral presentation on graduate research.

Karol Serafin-Molina (Silver Key lab) “Examining the Effect of Microbiota Removal in Drosophila Development and Alcohol Sensitivity” 8th Annual NCCU College of Science and Technology Research Symposium April, 2016

Misha Raza (Silver Key lab)” Comparing the Dot chromosome of Drosophila elegans to Drosophila melanogaster based on similarities in the amino acid sequences of the exons’ 8th Annual NCCU College of Science and Technology Research Symposium April, 2016. Note: Ms. Raza won 2nd place award ($75) for oral presentation on our research

Brianna McRae (SC Silver Key lab) “Comparing the Dot chromosome of Drosophila biarmipes to Drosophila melanogaster based on similarities in the amino acid sequences of the exons.” 6th Annual NCCU College

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of Science and Technology Research Symposium April, 2014. Note: Ms. McRae won 2nd place award ($100) for oral presentation on our research.

Denise Reaves (Silver Key lab) presented research entitled: Drosophila melanogaster study of non-pigmented adults due to Silver Nano-particle exposure at 4th Annual NCCU College of Science and Technology Research Symposium, April 13, 2012, Durham, NC.

Denise Reaves 1 , Tierra Poteat2, John Bang2, S. Catherine Silver Key1 Investigation of De-pigmentation Phenotype in Drosophila melanogaster Due to Silver Nanoparticle Exposure” 1Department of Biology & 2Environmental, Earth, and Geospatial Science, North Carolina Central University, Durham, NC 27707, April 1, 2011, CST NCCU Research Symposium, Student oral presentation. Note: Ms. Reaves won 2nd place award ($100) for oral presentation on our research.

Regional & Local Poster Presentations:

Kristen Smith, C. Swanson, L. Gavilano, M Snow-Smith, P Malek, RS Duronio, SC Silver Key presented research entitled: ‘Mutation of the lethal(2)denticless gene results in larval lethality and sterility’ at the 4th Annual NCCU College of Science and Technology Research Symposium, April 13, 2012, Durham, NC.

Jackie White 1 , Oladoyin Iranloye 1 , Roketa Sloan2, and S. Catherine Silver Key1, Analysis of Complementation Assay stocks for pHoP excitation in Drosophila melanogaster 1Department of Biology at North Carolina Central University, Durham, NC 2 Department of Genetics and Genomics, Duke University, Durham, NC, CST NCCU Research Symposium,April 1, 2011.

1 Maryonne Snow-Smith , 2Roketa Sloan, 1Lilly Gavilano, and S. 1Catherine Silver Key, Investigating the Male Sterility Phenotype caused by Disruption of the Drosophila Cdt2/l(2)dtl Gene 1Department of Biology, North Carolina Central University, Durham, NC 27707 2 Department of Genetics and Genomics, Duke University, Durham, NC CST NCCU Research Symposium, Student poster presentation April 1, 2011

Jackie White 1 and S. Catherine Silver Key1, Genomic Annotation of Drosophila mojavensis Contigs 26 and 27 using the D. melanogaster Genome as Reference Species 1Department of Biology at North Carolina Central University, Durham, NC, CST NCCU Research Symposium, April 1, 2011.

Ashlee G. Johnson, Maryanne A. Snow-Smith, Sheena D. Nichols,Tyneshia C. Powell, S. C.S Key.Biology Department, Mary Townes Science Complex, North Carolina Central University, Durham, NC 27707 “Gene Annotation of Contigs 7 and 10 of the Dot Chromosome (Chromosome 4) in Drosophila mojavensis”, NCCU Research Symposium, March 19, 2010Jackie White, Franziska Turner, and Dr. Catherine Silver Key, Department of Biology, North Carolina Central University, Durham, North Carolina 27707, “Genomic Annotation of Contig 21 in Drosophila Mojavensis, NCCU Research Symposium”, March 19, 2010

S. Catherine Silver Key (faculty) and Roketa Sloan (graduate student). “The Function of l(2)dtl in Drosophila melanogaster.” Annual Scientific Retreat for UNC Lineberger Compprehensive Cancer Center and NC Cancer Hospital, William and Ida Friday Center, Chapel Hill, NC May 20, 2009.

S. Catherine Silver Key (faculty), Roketa Sloan (graduate) and Maryonne Snow-Smith (ITSSTEM undergraduate). Mid-Atlantic Regional Society for Developmental Biology (SDB). University of Maryland at College Park, MD., May 15-16, 2009. Posters presented at Society for Developmental Biology meeting: 1. R.S. Sloan and S.C. Silver Key “The Function of l(2)dtl in Drosophila melanogaster.” (data was updated from ABRCMS meeting below).2. Snow-Smith and S.C. Silver Key “Detection of L2DTL mRNA in Drosophila Salivary Glands and Ovarioles.”

Roketa Sloan and S.C.S. Key, 4th Annual NCCU Graduate Research Day, poster presented: “The Function of

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l(2)dtl in Drosophila melanogaster.” NCCU, Durham, NC, April 3, 2009.

Sloan, R. and S. Catherine Silver Key, “The function of piggyBac l(2)dtl insert in Drosophila melagaster” NCCU Graduate Research Day, March 26, 2008.

Mollison, L. and S. Catherine Silver Key: “Loss of L2DTL Possible Contributes to Genomic Instability”, at ‘NCCU Graduate Research Day: Celebrating Research’, H.M. Michaux School of Education Building, March 26, 2008

Sloan, R. and S. Catherine Silver Key, The Function of piggyBac insert f04209 in Drosophila melanogaster” at ‘NCCU Graduate Research Day: Celebrating Research’, H.M. Michaux School of Education Building, March 26, 2008.

Mollison, L., Rowell, J., and S. Catherine Silver Key "Understanding the role of the L2DTL, in the G1/S checkpoint during Drosophila melanogaster development", March 20, 2007. NCCU/EPA Day, Mary Townes Science Building, Durham, NC

Mollison, L.* and S.C.S. Key. ‘Understanding the role of the E2F responsive gene, CG11295 [l(2)dtl], in the G1/S checkpoint during Drosophila melanogaster development.’ PMABS Summer Research Poster Session during the Undergraduate Research Symposium, Lineberger Comprehensive Cancer Center Atrium, University of North Carolina at Chapel Hill, NC. July 27, 2006.* Undergraduate in Partnership for Minority Advancement in Biomedical Sciences (PMABS) program

Taylor, S. *, S. C. Silver Key, R. J. Duronio ‘The Expression Pattern of the E2f1 Transcription Factor During Drosophila Development’ Ninth Annual Life and Physical Science Research Symposium February 26, 2004.

* note: an undergraduate in MARC or Partnership for Minority Advancement in Biomedical Sciences (PMABS) program at NCCU or NCA&T.

Grants, Awards, & Fellowships:

July 31, 2012 Awarded ABLE Laboratory Teaching Initiative Grant (LTIG) for application titled: “Gene Expression Patterns in Drosophila Embryos”, $1,055.

June 2012 Honorarium received for 60 questions submitted to National Science Bowl, $300.

Nov 2011 Honorarium received for 60 questions submitted to National Science Bowl, $300.

June 2011 Awarded extension of NIH/NIGMS SCORE SC2 grant until 2012.

Oct. 2008 ABRCMS Judges Subsidy Award: registration, food and hotel, Orlando, FL.

July 1, 2008 Awarded NIH/NIGMS SCORE SC2 grant: “L2DTL’s Function in Maintaining Genomic Stability during Drosophila Development”. $100,000 for first year: $225,000 total.

Sept. 5, 2007 Awarded one-year membership into the Council on Undergraduate Research.

May 18, 2007 Submitted: NIH/NIGMS SCORE SC2 pilot grant application entitled: L2DTL Function in Maintaining Genomic Stability During Drosophila Development” –

assigned a study section to be reviewed September or October 2007 – was funded.

April 4, 2007 Scholarly Achievement Award presented by NCCU Faculty Senate.

January, 2007 NCCU College of Science and Technology Outstanding Teacher Award.

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August, 2006 Inducted into local Chapter of Sigma Xi Research Society. (Membership current)

May 15, 2006 Awarded 1 year pilot from Extramural Associates Research Development Award (EARDA) proposal, Title: ‘Drosophila lethal(2)denticleless: Pursuing A Member of a New Family of DNA Replication Factors.’ EARDA Director at NCCU: Dr. Veronica Nwosu.

2002-2005 Seeding Postdoctoral Innovators in Research and Education (SPIRE) NIHFellowship under direction of Dr. Skip Bollenbacher and Dr. Leslie Lerea.

June 2004 Best Teaching Poster Award and Certificate at 4th Annual IRACDA Meeting, Emory University, Atlanta, GA, June 2004. Cash Award.

1990-1993 Graduate research funded by NIH training grant awarded to the Rheumatology and Immunology Division at UNC-Chapel Hill,Advisor: Dr. S.W. Hunt, III.

1988 Muriel E. Babcock Award for Excellence in Science, University of Missouri at St. Louis.

1985-1987 University of Missouri academic scholarships

1985 Pierre Laclede Honors Scholar, University of Missouri – St. Louis

Faculty Development Workshops:

Annual attendance at Workshops for Genomics Education Partnership since 2009-current.

NSF Day at UNC-Chapel Hill, April 19, 2017

Grant Writing workshop at NCA&T, Greensboro, NC, F2016

Quality Service Initiative Training Certification. NCCU, Fall 2012.

NSF SEA Grant Writing Workshop video teleconference with D.C., September 2012 St. Augustine’s College, Raleigh NC

August 9-12, 2012 Genome Education Partnership (GEP) 2012 Alumni Workshop.

June 19-22, 2012 Association for Biological Laboratory Education (ABLE) – attended 2 major laboratory workshops and 2 minor workshops.

April 13, 2012, 4th Annual NCCU CST Research Symposium, NCCU, Durham, NC

March 7-11, 2012, 54th Annual Drosophila Conference: Drosophila Genetics, Chicago, IL.

June 5-7, 2011 Genome Education Partnership (GEP) 2011 Alumni Workshop. Washington University, St. Louis, MO Learned about the model organism DNA encyclopedia (modENCODE) project – presentation by Dr. Sarah Elgin Learned about progress toward out collaborative GEP Science Education Research paper from Dr. David Lopatto – Dr. Key will be an author on the papero Worked on GEP Faculty Survey and Student Author Survey and discussed other tools for collecting data in committee format. Learned of progress toward Dr. Key and NCCU genomics collaborative Genomics Research paper comparing annotation of Drosophila mojavensis and D. grimshawi dot chromosome in comparison to D. melanogaster – stu-dents will be an author on the publication – aiming for 2012.

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o Dr. Key and several NCCU students have submitted annotation reports on Drosophila mojavensis.

March 25-26, 2011 North Carolina Academy of Sciences (NCAS) Annual Conference, Heard latest on transplantation applications Dr. Atlata lecture, one student poster,

1 oral presentation, networked and learned about ogoeing research throughout North Carolina, Elon University, Elon, NC

S2011 EAGLES Purchase Training Learned how to used new system to create requisitions D. Hearst

March 18, 2011 Quantitative Real-time PCR Bio-Rad Workshop Learned the value of multiple control templates per run: include 2-3 such as actin, rp49, and tubulin.from Jo Vandesompele(European) and Francisco Bizouarn

August 2010 Genome Education Partnership (GEP) Alumni Workshop – I worked to revamp the GEP quiz and survey that we administer to students using GEP materials to learn genomics/bioinformatics in the classroom and learned new

features of the websites and future plans for the groups to implement ChiP research after sequencing and annotation research is completed in this Nation-wide collaborative. August 13-15, Washington University in St. Louis

March 18, 2011 Quantitative Real-time PCR Bio-Rad Workshop at UNC-Chapel Hill with Francisco Bizouarn and Jo Vandesompele. Learned the value of multiple control templates per run: include 2-3 such as actin, rp49, and tubulin.

March 25-26, 2011, attended and presented at 108th North Carolina Academy of Sciences meeting.

April 7-10, 2010 51st Annual Drosophila Conference Washington University in St. Louis

June 6-11, 2009, Genomic Education Partnership (GEP) Workshop One week of intensive training to learn Consed, BLAST, and Genome Browser and other programs needed for comparative genomics projects. NCCU students have been introduced to these bioinformatic research techniques in BIOL4550, BIOL3100 and BIOL4400, Washington University in St. Louis.

May 15-16, 2009 70th Mid-Atlantic Society for Developmental Biology (SDB), College Park, MD

Nov. 5-8, 2008 ABRCMS Sessions on ‘The Key Practices of Exemplary Leaders: Managing Self to Lead Others” by Delorese Ambrose, Ed.D., Judges Orientation for Molecular Biology Judges, ‘NIH Grants Management Workshop’, Networking meals and reception, plenary sessions on Nanomaterials and Health, Evolution of Sex, How Bacteria Communicate, and Synthetic Biology is our future.

June 3-7, 2008 30th Association for Biology Laboratory Education Conference (ABLE), University of Toronto at Mississauga, Canada. Bioinformatics and Genetics lab workshops

Feb 5, 2008 ‘Assessing Your Service Skills” NCCU Chancellor’s Quality Service Initiative (QSI) workshop, 2-4 p.m. School of Law – assessment taken.

July 19-23, Council for Undergraduate Research (CUR) Proposal Writing Institute at University2007 of Nebraska, Kearney. Facilitator: Paula Dehn of Canicius College, New York. Proposal:

NSF URM for ‘Mentoring Molecular Biology Students in Research’ – not submitted.

July 9-13, Genome Consortium for Active Teaching (GCAT) Summer Microarray 2007 NSF Funded Workshop at Morehouse College, Atlanta, GA. Trained in wet lab and dry lab

microarray procedures that can be used in the classroom or research with undergraduates.

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June 5-9, Attended the 29th Annual Association for Biology Laboratory Education2007 (ABLE) at University of Kentucky, Lexington KY. ABLE is an international organization. Participant in

four major hands-on laboratory workshops in Genetics. Reviewed two labs for publication in ‘Proceedings: Tested Studies for Laboratory Teaching”.

Spring 2007 Student Learning Outcomes (S.L.O.s) Workshop, Dr. Janice Harper in preparation for SACS accreditation, Mary Townes Science Building Rm1111, NCCU, Durham, NC SLOs are now incorporated into BIOL3100 syllabus.

Jan 2007 NCCU Faculty Development Workshop, New School Ed.

Sept, 15-16, Quality Education for Minorities (QEM)/BIO NSF Grant Writing Workshop:2006 Nomination required and summary grant proposal submitted to be invited.

August, 12-17 FASEB Summer Research Conference: Transcriptional Regulation during2006 Cell Growth, Differentiation, and Development. Saxtons River, VT.

Aug. 9, 2006 BANNER version 7.2 Training, Mary Townes Science Complex

May 17, 2006 BANNER Finance Training to manage grant funds, led by: Sheila Stone.

May 11, 2006 TELS (Technology Enhanced Learning in Science) workshop with Drs. Gail Hollowell and Cassandra Palmer, Tun Nyein and Sandra White

March 13-14, NIGMS Internet Grant Writing workshop, University of Kentucky at 2006 Lexington, contact person: Margaret McConnell for Hinda Zlotnik

Jan. 4, 2006 Three workshops: ‘Electronic Portfolios for Faculty Evaluation’, ‘Diversity in the Classroom’ and ‘The Purposes of Learning, Assessment, and Test-Item Development’

from Center for University Teaching and Learning’s Professional Development Day: Enhancing Teaching and Learning through Collaboration - School of Education Faculty

Dec. 3, 2004 ‘Bringing Technolgy into the Classroom – Pros and Cons’ workshop by Dr. Jory Weintraub, Dr. Brian Rybarczyk, and Mr. Michael Cato of the Partnership for Minority Advancement in the Biomedical Sciences (PMABS), UNC at Chapel Hill.

Sept. 2004 ‘Science Education Research’ Workshop by Dr. Richard Felder of NCSU and Rebecca Brent, UNC at Chapel Hill: Carolina Inn.

Nov. 15, 2003 Effective College Teaching Workshop by Richard Felder (North Carolina State University) and Rebecca Brent at UNC at Chapel Hill

Nov. 14, 2003 NSF Science Technology, Engineering and Mathematics (S.T.E.M) workshop at North Carolina A&T State University led by Ruth (MIMES,CO) and Leon Richards.

Fall 2002 SPIRE Teaching Workshop Series, Center for Teaching and Learning (CTL)UNC at Chapel Hill. Instructor: Ed Neal, director of CTL.

Jan. 18-19, Effective College Teaching Workshop by Richard Felder and Rebecca Brent at IRACDA2002 National Meeting, University of North Carolina at Chapel Hill

July 23-27, Elon Univerity A.C.S. Summer Workshop: Web-based Learning (Blackboard)2001

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June 11-21, Elon University Academic Computing Services (A.C.S) Summer 2001 Workshop: Web-page Design (FrontPage)

International, National, University, College, and Departmental Service:

F2012-current. Duke University Preparing Future Faculty (PFF) mentees: Dr. Laura Williams, 2012-2013 Dr. Jessica Sawyer, 2013-2014 Brenna Forester, 2014-2015 Dr. Barbara Sixt, 2015-2016 Nichole Orench-Rivera 2017-2018

2012-2014 co-chair with Dr. R. M. Richardson for D.O.B. Peer Evaluation Committee

2012-current chair CA&S Strategic Planning Committee

2012-2015 Faculty Senator Alternate to Dr. Amal Abu-Shakra

Sum’11& 12 FUTURES research mentor, Bianca Harris, Jackie White and Tashonda Smith

Spring2011 RISE research mentor, Jackie White

F2010-current S-STEM research mentor, Jackie White (F2010), Kristen Smith (F2011-current) & Denise Reaves (F2010-F2012)

2008-2010 co-chair with Dr. J Clamp for D.O.B. Peer Evaluation Committee

2006-current Chair of NCCU/UNC-Chapel Hill SPIRE Program Committee. Recruiting and mentoring Post-doctoral fellows in teaching at NCCU: Seeding Post-doctoral Innovators in Research and Education (SPIRE) liaison. Biology, Chemistry and Physics Department involvement. Managing SPIRE subcontract.

Sept. 2009 Abstract Reviewer for 2010 ABRCMS

Jan-Aug2008 ITTSTEM Research Mentor – undergraduate students Brian Shackleford and Maryonne Snow-Smith. Director: Dr. Tokuta and Crystal Gathers, at NCCU, Durham, NC. Students

2006-2009 MARC Research Mentor for Lonna Mollison (now at UNC-Chapel Hill Ph.D. program F2008-current)) and Janelle Rowell, (now Johns Hopkins Ph.D.program)

July 2007 ITSSTEM Research Mentor – undergraduate student La’Donte Garrett Director: Crystal Gathers, at NCCU, Durham, NC Rotation report was submitted.

Su2006 Research mentor at UNC-Chapel Hill PMABS Summer Research Program. Dr. Brian

Rybarczyk, director and Ms. Lonna Mollison NCCU

Nov. 5-8 Molecular Biology Judge, ABRCMS, Orlando, FL: 5 poster sessions and 2008 one oral session, with three students in each session.

June 2008 Taught professional development sessions for college professors at 30th ABLE Conference, Toronto, ON: “Gene Knockout/Gene Therapy in Yeast Using Homologous Recombination”.

June 2008 Peer-reviewer for ABLE Proceedings: Tested Laboratory Studies for Teaching.

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Spring 2008 Appointed as Nominations Committee chair person for Faculty Senate.

April 2008 Peer-evaluation committee member for Biology Department.

Spring- Mentor for SPIRE post-doc, Dr. Jackie Swanik. Duties include guide and Fall 2008 evaluating her teaching in BIOL4100, Inquiries in Developmental Biology, and training in other faculty responsibilities.

June 2007 Peer-reviewer for ABLE Proceedings: Tested Laboratory Studies for Teaching.

Dec 4, Scientific Judge for Ms. Rittman’s course Heredity and Society, BIOL1000. 2007 Debate Topics included: Ethics of using Stem Cells to Cure Disease and the ‘What Effects Intelligence more: Genetics or Environment?”

Oct. 2006- Served as co-chair with Dr. Myers (chemistry) College of Science and April 2007 Technology (CST) Strategic Planning Team as formed by Dean Caesar Jackson. Mission of CST generated and approved by faculty April 2006.

Proposed Vision, Goals and Objectives for CST provided to Dean.

F2006-S2009 Faculty Senator, one of three Department of Biology representative.

Spring- Partnership for Minority Advancement in Biomedical SciencesSum 2006 (PMABS) co-director with Dr. Amal Abu-Shakra.

2005-current Undergraduate Curriculum Development Committee at NCCU

2005-current Point Person for Biology Teaching Labs at NCCU

Fall 2005 Seeding Post-doctoral Innovators in Research and Education (SPIRE) mentor to Dr. Traci Powell in Genetics BIOL3100.

Spring 2005 5th Annual Distinguished Scholar Seminar committee member, hosting Dr. Shirley Malcolm at UNC-Chapel Hill

2005-2006 Seminar Committee Member at North Carolina A&T State University

Jan. 2005 Committee Member for 9th Annual Life and Physical Science Research Symposium, featuring Dr. Francis Collins and Human Genome at North Carolina A&T State University

2004-2005 Curriculum Developmnt Committee at North Carolina A&T State University

Spring 2004 4th Annual Distinguished Scholar Seminar committee member, undergraduate recruiter at North Carolina A&T State University

20023rd Annual Distinguished Scholar Seminar SPIRE committee member

Community Service:

June 2017 Provided peer-reviews for 4 major workshops for publication at Association for Biology Laboratory Education (ABLE), University of Wisconsin, Madison.

June, 2012 Genetics Question Writer for National Science Bowl competition: 60 questions (8 hours) submitted to Oak Ridge Associated Universities

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April 13, 2012 Scientific Judge for 9 posters at 4th Annual NCCU CST Research Symposium (1 hour), January 28, 2012Scientific Judge for NC Regional Science Bowl (8 hours), Durham, NC

February, 2012. Hosted five SPIRE Scholar seminars

November 23, 2011. Genetics Question Writer for National Science Bowl competition: 60 questions (8 hours) submitted to Oak Ridge Associated Universities.

June-July 2012 Authored a vignette ‘Don’t Drink and Fly’ for Science/AAAS Magazine’s educational website to introduce A.P. High School students and freshman college students to the modENCODE project chromatin modification to a public website. Scientists us the site,

but the goal of the service project is to introduce students to the site and the power of studying model organism so they can use the data to conduct their own research. Beta site up (confidential). Completed site due to be available September 2012.

April 8, 2011 Southern Middle School Drosophila Research presentations to 174 students

Oct., 20, 2010 Research Project in Drosophila at NCCU presented to NCSSM High School.

Spring 2008 Visiting Scientists Lecture NCSSM, Durham, Dr.Christine Muth’s Molecular Genetics Course: “Retinoblastoma and Ubiquitin Ligases In Flies and Humans”

2007-current First Aider for Mary Townes and Girl Scouts: CPR and First Aid Red Cross training.

2007-current Junior and Cadette Girl Scout leader of Troop 40668. Activities involve weekly badge workshops: Eco-Action, Making it Matter (invited physicist Dr. Diane Markoff of NCCU to teach girls), Math Whiz, Girl Scouting Around the World (global awareness), Thinking Day badge (where we learned about water conservation in Haiti, Morocco, and Sierra Leone from a returned Peace Corp Volunteer), and a Junior Aid Badge/Cadette Interest Projects and Journeys in which Scouts taught younger girls about water conservation. Cadettes earned service project to aid Carolina Tiger Rescue.

Jan (one Sat./year)2006-2012 Biology Judge for NC Regional High School Science Bowl, NCCU High School student

competition, Mary Townes Science Complex coordinated by Dr. Fredrick Johnson.

September Oral and hands-on presentation “Protein Nutrition in Kids and Fruit Flies” at South2007 Graham Elementary, Mrs. Wall and Mrs. Hall’s 4th grade classes in the Alamance.

June 2006 Summer Camp Unit Leader for Girl Scout, Tarheel-Triad Council: Alamance Twilight Camp, Theme: Exploring New Directions Trained 14 Brownies, 2 Junior Leaders and 1 program aid on following: 1.)Use of compass; 2.)Tree and plant identification (leaves); 3.) Microscopic examination of algae and protozoa living in water; 4.) Mathematical measurement and construction of aerodynamic crafts: rocket, glider, and pinwheel; 4.) Beginner swim-ming activities; 5.) Agriculture: seed growth race

2007-2012 Junior and Cadette Girl Scout Leader of Troop 40668. Girls completed Bronze and Silver service Awards at 30 hours and 60 hours respectively.

2005-2007 Brownie Girl Scout (GS) Leader of Troop 668, Girl Scout Tarheel Triad Council, Alamance County: Graham United Methodist Church Weekly meetings plus weekend fieldtrips Activities: Try-It Activities include:

Science & Nature badge work Business, music, good citizenship badges. GS Law: promoting confidence in girls in 1st-3rd grade;

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Field trips include: camping, science wonders, hiking, etc.

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€¦ · web view30/04/2018 · able rwltig proposal 2018 s. catherine s. key, at nccu (hbcu)...

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