utilizing powerpoint effectively
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Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Admitting that there is a problem …
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with “compelling material”.
“Substance” vs. “Style”
Putting PowerPoint to Work for You
“Substance” vs. “Style”
Putting PowerPoint to Work for You
HRC PH
X
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Putting PowerPoint to Work for You
Ooooouuuuu….
Aaaaaahhhhhhhhhhhhhhh…
LOOK @ ME…
I am Special… with a capital S…
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
If you utilize slide transition animations:
Be consistent from start to finish
Shy away from sounds
And…
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides & text.
Putting PowerPoint to Work for You
The competition is rough...
Putting PowerPoint to Work for You
Then…
Constructing a PowerPoint presentation…is like preparing for a backpacking expedition.
•Plan ahead. •Review your goals.•Plan for emergencies.
Putting PowerPoint to Work for You
TEXT: Less > More...
Sentences :o(
Bullet Points :o)
Consistent Style
Key Idea Only
Putting PowerPoint to Work for You
6 X 6
Putting PowerPoint to Work for You
Where are we going and why?
Outline the itinerary.
Give some background info.
Get the tour bus moving.
Don’t be afraid to pull the bus over.
Bring it back to the beginning.
Think APA writing format…
Think tour bus…
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Putting PowerPoint to Work for You
Step 4: Don’t parrot PowerPoint.
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Step 5: Time your remarks.
Putting PowerPoint to Work for You
About 100 words per minute…
Repeat questions posed to you…
It is OK to pause before you answer a question…
Keep a healthy pace but keep in mind that if the audience senses that you are rushing...
Time your remarks…
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Step 5: Time your remarks.
Step 6: Give it a rest.
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Step 5: Time your remarks.
Step 6: Give it a rest.
Step 7: Careful with fonts and backgrounds.
Some fonts are:
– really ugly– hard to read and annoying– suitable for comics– More science fiction than
scientific
Careful with fonts styles…
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
Arial
Sans Serif
Times New Roman
The Big 3
Putting PowerPoint to Work for You
Oh so temping…
You don’t design ads for
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
Backgrounds
Ask yourself:•Does it help the presentation?
•Work with my text & graphics?
•Does it seem out of place?
•Will they wonder if I forgot to take my meds?
Making PowerPoint work for you.
A 10 Step Program of sorts
Putting PowerPoint to Work for You
The competition for the audience’s attention is rough.
Don’t give them another excuse to tune you out!
Are You Kidding Me?
Putting PowerPoint to Work for You
The beauty of “white space”…
Clean & crisp
Text pops off the page
Keeps the focus on your message
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
Putting PowerPoint to Work for You
Final word on backgrounds …
Ok, white space is not always best
Think contrasting colors
Think readability & clarity
Then… Try it out
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Step 5: Time your remarks.
Step 6: Give it a rest.
Step 7: Careful with backgrounds and fonts.
Step 8: Import images, graphics and other media
Putting PowerPoint to Work for You
Thank you, Al Gore …
Putting PowerPoint to Work for You
Got images?
Putting PowerPoint to Work for You
RE: Graphs...
2-D vs. 3-DAdios grid-linesContrast with colorWider is betterProvide the punch-line
Note the increase in mean GRE scores
1050
1100
1150
1200
1250
1300
1350
2006 2007
Biochemistry
Microbiology
MolecularMedicine
Neuroscience
For entering studentsN = 36 in 2006N = 33 in 2007
Putting PowerPoint to Work for You
It took centuries to decipher the Rosetta stone…
Your audience has 1 minute…
Putting PowerPoint to Work for You
Good slide vs. poor slide…
Sometimes…
the difference is subtle…
• Fatty acid-binding protein (FABPs) belong to a family of intracellular lipid binding proteins of low molecular mass (14–15 kDa) with the putative general function of lipid trafficking (1).
• The precise physiological functions of these proteins are as yet unclear, but it is hypothesized that they are important in intracellular transport and targeting of FA to specific membranous organelles and metabolic pathways.
• Intestinal fatty acid-binding protein is abundantly produced in the enterocyte, where a second fatty acid-binding protein (FABP), liver FABP (LFABP), is also highly expressed (2)
Fatty Acid binding proteins
• Fatty acid-binding protein (FABPs) belong to a family of intracellular lipid binding proteins of low molecular mass (14–15 kDa) with the putative general function of lipid trafficking (1).
• The precise physiological functions of these proteins are as yet unclear, but it is hypothesized that they are important in intracellular transport and targeting of FA to specific membranous organelles and metabolic pathways.
• Intestinal fatty acid-binding protein is abundantly produced in the enterocyte, where a second fatty acid-binding protein (FABP), liver FABP (LFABP), is also highly expressed (2)
Fatty Acid binding proteins
• family of intracellular lipid binding proteins
• low molecular mass (14–15 kDa)
• putative function in intracellular lipid trafficking
• tissue specific expression of different FABPs• intestine expression both L-FABP and I-FABP
• the functional roles of these FABPs in lipid absorption are as yet unclear
• may regulate FA traffic among organelles
Fatty Acid Binding Proteins (FABPs)
• family of intracellular lipid binding proteins
• low molecular mass (14–15 kDa)
• function in intracellular lipid trafficking
Fatty Acid Binding Proteins (FABPs)
Putting PowerPoint to Work for You
It a scary world out there…
In our lecture halls…
Neuromuscular Junction(Motor Neuron to Muscle Fiber Synapse)
Freeze fracture cleaves membranes in hydrophobic plane of bilayer
Electron micrograph
Vesicle Trafficking and ExocytosisLearning Objectives:1. Systems used to study vesicle fusion & exocytosis
a. Yeast secretory mutantsb.* C. elegans movement mutants (uncoordinated)c.* Drosophila mutantsd. Cell free Golgi vesicle trafficking assaye. Protein purification, antibodies, interacting proteins
[synaptic preps: Torpedo, brain]f. Synaptic preparations for electrophysiology
- squid giant synapse• SNARE hypothesis
a. NSF, SNAPs and SNAP Receptors• Identity of t- and v- SNARES• SNARE zipper mechanism (postdocking)• Role of NSF & SNAPs
• Very rapid, Ca-dependent exocytosis at synapses
• Ca sensor: Synaptotagmin
Unsaturated fatty acids inhibit 2nd phase.
Sphingolipid and cholesterol cause distinct phase to form in pure lipid mixtures containing
phospholipid with saturated fatty acid chains
Mix DOPC (unsat’d) or DPPC (sat’d) at different ratios, with sphingolipid +/- cholesterol, at 23oC or 37oC. Assay by fluorescence quenching of marker that only sees sol phase lipid. Phase separation indicated by break in slope of quenching curves.
See 2nd phase at 23oC with increasing DPPC .
Need cholesterol to obtain 2nd phase at 37oC.
Cholesterol promotes 2nd phase formation
Human Genome Project1990-2003
HAPMAP2002 –
http://www.hapmap.org/
ENCODE Project 2003 -
ENCyclopedia of DNA Elements:identify all functional elements in the human genome sequence
compare the genetic sequences of different individuals to identify chromosomal regions where genetic variants are shared.
3: Examine Region
Chromosome-wide summary data is
shown in overview
Default tracks show HapMap genotyped SNPs, named genes
from Entrez, and alternative mRNA splicing patterns
Electricalactivationof skeletal
muscle forceproduction
Single stimulus, single twitch
Multiple stimuli, unfused or fused tetanus
Prolonged depolarization,long contracture then relax( Not physiological )
Force
Force
Force
AP
Time
Putting PowerPoint to Work for You
There is hope.
Overview
1. ESI mass spectrometry
2. Protein-Protein Interaction and Pathways
high voltagehigh voltage
samplesample
vv11
m1
vv22
m2
vv33
m3
m1 m2 m3
detectordetector
drift regionlaserlaser
Matrix-assisted Laser Matrix-assisted Laser Desorption/Ionization (MALDI)Desorption/Ionization (MALDI)
Time-of-Flight (TOF) AnalyzerTime-of-Flight (TOF) Analyzer
MALDIMALDI
•Differentiation - need to generate multiple cell types•Morphogenesis - cells need to be spatially organized
How do you generate an organism from a single cell?
in situimmuno
How do we experimentally identify a tissue or cell type?
1. Histology
smooth muscle nerve cells
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Step 5: Time your remarks.
Step 6: Give it a rest.
Step 7: Careful with backgrounds and fonts.
Step 8: Import other images, graphics and other media.
Step 9: Distribute handouts at the end.
Putting PowerPoint to Work for You
A 10 Step Program of sorts…
Step 1: Hold up your end with compelling material.
Step 2: Keep it simple.
Step 3: Minimize the number of slides and text.
Step 4: Don’t parrot PowerPoint.
Step 5: Time your remarks.
Step 6: Give it a rest.
Step 7: Careful with backgrounds and fonts.
Step 8: Import other images, graphics and other media.
Step 9: Distribute handouts at the end.
Step 10: Edit ruthlessly before presenting.
Putting PowerPoint to Work for You
Get acquainted with the F7 key.
Employ the eyes of others.
If your not sure… cut it out.
Don't speak without rehearsing.
Even Seinfeld rehearses and…
He has nothing to say…
Promoting Excellence in Biomedical Education
lifesciences.umaryland.edu