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USGS science for a changing world Long-Term Patterns of Vegetation and Salinity Change in the Lake Pontchartrain Region, Louisianc U.S. GEOLOGICAL SURVEY OPEN-FILE REPORT 00-520 Debra A. Willard, Thomas M. Cronin, Scott E. Ishman, Marci Marot U.S. Department of the Interior U.S. Geological Survey

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Page 1: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

USGSscience for a changing world

Long-Term Patterns of Vegetation and Salinity

Change in the Lake Pontchartrain Region, Louisianc

U.S. GEOLOGICAL SURVEY OPEN-FILE REPORT 00-520

Debra A. Willard, Thomas M. Cronin, Scott E. Ishman, Marci Marot

U.S. Department of the Interior U.S. Geological Survey

Page 2: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

LONG-TERM PATTERNS OF VEGETATION AND SALINITY CHANGE IN THE

LAKE PONTCHARTRAIN REGION, LOUISIANA

Debra A. Willard 1 , Thomas M. Cronin 1 , Scott E. Ishman2, Marci Marot3

'U.S. Geological Survey, 926A National Center, Reston, VA 20192

2Dept. of Geology, Southern Illinois University, Carbondale, IL

3U.S. Geological Survey Center for Coastal and Marine Geology, 600 4th St. South,

St. Petersburg, FL 33701

Abstract

Palynomorph, ostracode, and benthic foraminiferal assemblages from Lake Pontchartrain sediment cores were analyzed to reconstruct long-term trends in regional vegetation and salinity in the lake. Pollen assemblages from two short cores (9-7-96-7 and 9-7-96-9), mostly deposited after 1950 AD, indicate dominance ofPinus pollen and the common occurrence of Pediastrum, a colonial green alga typically indicative of eutrophication, throughout this time. A longer core (3.47 m), LP 97-la, includes middle Holocene sediments (5.5-4.2 ka), characterized by higher salinities and greater abundance of Quercus pollen. Younger sediments in this core record a transition to meso-to oligohaline conditions and wetter conditions later in the Holocene. The upper 30-40 cm of LP 97-la represents sediment deposition after 1950 AD. These sediments are characterized by oligohaline to limnic ostracode faunas and unprecedented abundance of colonial green algae indicative of eutrophication. This may represent the response of phytoplankton and benthic communities to the introduction of Mississippi River water and sediment resuspension resulting from openings of the Bonnet Carre' spillway during the last half of the 20th century.

Introduction

As part of a project documenting the impact of altered land management on Lake Pontchartrain, Louisiana, a series of cores was collected for analysis of trace metals and reconstruction of the vegetation and salinity histories of the lake and surrounding region. This report describes pollen records from three cores collected in Lake Pontchartrain and data from ostracode and benthic foraminiferal assemblages from one core to determine the feasibility of documenting both the climatic variability of the system and its response to anthropogenic changes of the last few decades and centuries.

Methodology

Palynomorph assemblages were examined from three cores from Lake Pontchartrain (Figure 1, Table 1), and ostracodes and benthic foraminifers were studied from one core,

1

Page 3: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

TABLE 1. Locations of cores analyzed for palynomorphs, Lake Pontchartrain, Louisiana.

CORE ID

9-7-96-7

9-7-96-9

LP-97-la

LATITUDE

30.0775° N

30.1 105° N

30.253 1°N

LONGITUDE WATER DEPTH (m)

90.0363° W

90.1051° W

90.1 597° W

5.03

4.88

4.88

CORE LENGTH (cm)

62

59

347

LP-97-la. Sediment samples weighing from 5 to 15 grams were used for pollen preparation. Samples were dried and weighed before being spiked with Lycopodium marker tablets for calculation of absolute pollen concentrations (Stockman, 1971). Samples were treated with HC1 to remove carbonates, neutralized with deionized water, and treated with HF to remove silicates. After neutralization, the samples were sieved through 200 pm and 10 |0,m nylon mesh to remove extraneous plant material and clay- sized particles. Some samples were run through a heavy-liquid separation with ZnCl2 (S.G. = 1.8) to further clean samples. The pollen residue was mixed with warm glycerine jelly and mounted on microscope slides for examination.

To calculate percent abundance of pollen, at least 300 grains were counted from each sample; these results are provided in Appendices 1-6. Absolute pollen concentrations were calculated using the marker-grain method described by Benninghoff (1962). The

90°30' 90°20' 90°10' 90°OCT 89°50' 89°40'

29°501

Figure 1. Location of core sites, Lake Pontchartrain, Louisiana.

Page 4: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

quantity of Lycopodium spores in the marker tablets was determined by the manufacturer with a Coulter Counter following the procedures of Stockmarr (1973) to be 12,542 +/- 416. Absolute pollen concentration was calculated using the formula (Maher, 1981):

pollen per gram dry sediment = ((pollen grains counted/markers counted) x 12,542)7weight of sediment.

Fossil pollen assemblages were compared statistically to modern assemblages from shallow marine sediments collected off the Gulf Coast of Mississippi and Alabama (Edwards and Willard, in press) and shelf assemblages collected along the east coast of North America (Litwin and Andrle, 1992; Willard, unpub. data) using the modern analog technique (MAT) (Overpeck, Webb, and Prentice, 1985).

Ostracode and foraminiferal assemblages were studied after washing sediment through a 63 jim sieve and qualitatively examining the assemblages under a binocular microscope. Ecological inferences were made based on published studies of foraminifers and ostracodes from Gulf and Atlantic coastal zones (Poag, 1978; Poag, 1981).

Three foraminifer samples for radiocarbon dating were obtained from LP 97-la, and results are listed in Table 2. Samples from 9-7-96-7 and 9-7-96-9 were analyzed for 210Pb and 137Cs activity. In 9-7-96-9, 137Cs is present throughout the core, indicating deposition of the entire core since 1950 AD (Flocks, et al, in press). In 9-7-96-7, 137Cs is present only in the upper 40 cm (Appendix 7), indicating that the lower part of the core (40-56 cm) was deposited before 1950.

TABLE 2. Radiocarbon dates from core LP 97-1. WW indicates laboratory numbers assigned by the USGS 14C Laboratory. Samples were processed at the 14C laboratory of the U.S. Geological Survey in Reston, Virginia. 14C ages were determined at the Center for Accelerator Mass Spectrometry (CAMS), Lawrence Livermore National Laboratory, Livermore, California. 513C values were estimated. 14C ages are reported with one sigma error.WW

1891

1892

1893

Depth (cm)

140-144

222-224

320-324

Material

Forams

Forams

Forams

613C°/

00

0

0

0

14CAge

yrBP

4,220

4,550

5,070

+/-

50

50

50

Calibrated age Cal yr BP*

4,426-4,152

4,846-4,602

5,562-5,299

Calibrated using CALIB 4.0 Radiocarbon Calibration software, after Stuiver and Reimer (1993), in conjunction with The Marine Calibration Data Set (Stuiver, and others, 1998). Calibrated age ranges are reported with a two-sigma error.

RESULTS

CORE 9- 7-96-7

Palynomorph assemblages were examined from every 2-cm interval in the upper 12 cm of Core 9-7-96-7 and every 5 cm below that. Pinus (see Table 3 for common names

Page 5: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

of plant taxa) pollen dominates most assemblages, typically comprising 60% to 90% of the assemblages (Fig. 2). Other tree pollen commonly present includes Quercus, Taxodiaceae/Cupressaceae/Taxaceae (TCT), Carya , and Liquidambar. Pollen of the herbaceous Chenopodiaceae/Amaranthaceae usually ranks second in abundance, comprising up to 40% of the assemblages, and Asteraceae pollen also is common. Above 40 cm depth, pollen concentration increases fivefold, and Pediastrum, a colonial green alga, is present in concentrations of up to 18,000 grains/gram dry sediment; below 40 cm, it is absent.

0204060800 0 0 0 020

Percent Abundance of Pollen

0 10 20 30 0 5 1015

Total pollen/gram Pediastrum/gram dry sediment (x1 o3) dry sediment (x1 o3 )

Figure 2. Percent abundance of pollen of major plant groups, core 9-7-96-7, Lake Pontchartrain, Louisiana. TCT - Taxodiaceae/Cupressaceae/Taxaceae.

CORE 9- 7-96-9

Palynomorph assemblages were examined from every 2-cm interval of the 48 cm long core, and assemblages vary little. Pinus pollen dominates most assemblages, com­ prising 33-69% of the assemblages (Fig. 3). Other common pollen types include Quercus, TCT, and Chenopodiaceae/Amaranthaceae, each of which typically comprise about 10% of the assemblage. Pediastrum is common throughout the core, with peak abundances at 30-35 cm and 6-12 cm.

Page 6: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

s

0 10 20 30 40 50 60 010 0 10 0

Percent Abundance

0 10 20 30 40 50 60 0 5 10 15 20 25

Total Pollen/gram PedJasfrurtVgram dry sediment (x103) dry sediment (x103)

Figure 3. Percent abundance of pollen of major plant groups, core 9-7-96-9, Lake Pontchartrain, Louisiana. TCT - Taxodiaceae/Cupressaceae/Taxaceae.

COREL? 97-la

Palynomorphs, ostracodes, and benthic foraminifers were examined every 5 cm in the upper meter of core and every 20 cm for the lower 2.5 meters. The basal 10 cm of core consists of a burrowed, Pleistocene clay with low concentrations of pollen. The upper 337 cm of core consists of Holocene sediments and is divided into four assemblage zones based on pollen, dinocysts, green algae, ostracodes, and benthic foraminifers. Zone I, between 340 cm and 90 cm depth (Fig. 4), is characterized by dominance of Pinus pollen (53-81%) and the highest abundances of Quercus in the core (9-21%). Dinocysts are common, comprising up to 2,600 cysts per gram; the dominant species is Polysphaeridium zoharyi, with Spiniferites spp. also common (Fig. 5). Ostracode and benthic foraminiferal faunas in this interval are comprised of polyhaline taxa (Fig. 6). Benthic foraminifer faunas have affinities to the Elphidium assemblage of Poag (1981), described from bays and lagoons of the Gulf Coast region. Common ostracodes in the assemblage include Paratocytheroma stephensi, Cytherura radialiratay Megacythere repexay and Cytheromorpha paracastanea.

Zone II, ranging from 90 to 50 cm depth, is characterized by faunas associated with mesohaline conditions (5-18 ppt). Foraminiferal assemblages are dominated by Ammonia parkinsonianay with abundant Elphidium gunteri. Ostracode assemblages are dominated by Perissocytheridea brachyforma, Candona spp., and, in the upper part of this interval, non-marine cyprids that indicate periodic fresh-water events. Pollen assem­ blages in this interval are characterized by much higher abundances of Chenopodiaceae

Page 7: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

Table 3. Scientific and common names of plant taxa preserved in the pollen record, Lake Pontchartrain, Louisiana.

TREES AND SHRUBS FamilyAceraceaeBetulaceaeBetulaceaeFagaceaeUlmaceaeRubiaceaeCornaceaeCorylaceaeFacaceaeOleaceaeAquilifoliaceaeJuglandaceaeHamamelidaceaeMagnoliaceaeMagnoliaceaeMyricaceaeNyssaceaeBetulaceaePinaceaePinaceaeFagaceaeSalicaceaeTaxodiaceae/Cupressaceae/TaxaceaeUlmaceae

HERBACEOUS TAXAAsteraceaeApiaceaeAsteraceaeChenopodiaceae/AmaranthaceaeCyperaceaeCyperaceaeEphedraceaeEricaceaeEuphorbiaceaeFabaceaeCelastraceaeLamiaceaeHaloragidaceaeNymphaeaceaePlantaginaceaePoaceaePolygonaceaeAlismataceaeTyphaceaeLentibulariaceae

GenusAcerAlnusBetulaCaryaCeltisCephalanthusCornusCorylusFagusFraxinusIlexJuglansLiquidambarLiriodendronMagnoliaMyrlcaNyssaOstrya/ CarpinusPiceaPinusQuercusSalix

Ulmus

Ambrosia

Cladium

Ephedra

Hippocratea

MyriophyllumNymphaeaPlantago

SagittariaTyphaUtricularia

Common NameMapleAlderBirchHickoryHackberryButtonbushDogwoodHazlenutBeechAshHollyWalnutSweet gumTulip treeMagnoliaWax myrtleTupelo/Black gumHophornbeamSprucePineOakWillowCypress/Cedar/Yew families

Elm

RagweedUmbel/parsley familyAster/daisy familyPigweed/Goosefoot familiesSawgrassSedge familyMormon teaHeath familySpurge familyBean/legume familyn/aMint familyWater milfoilWaterlilyPlantainGrass familyKnotweed familyArrowheadCattailBladderwort

Page 8: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

IV

4846-4602 I

5562-5299 I

CLAY

LJ

^ tf *r

0 10 20 30 40 50 60 70 0 10 0

Percent Abundance

Figure 4. Percent abundance of pollen of major plant groups, core LP 97-la, Lake Pontchartrain, Louisiana. TCT - Taxodiaceae/Cupressaceae/Taxaceae.

pollen, with increased percentages of other herbaceous taxa, including the Poaceae, Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem­ blage in this interval, although its concentrations are low (<300 per gram).

In zone III (50-30 cm depth), the transition from mesohaline to oligohaline (1-5 ppt) conditions is documented, with salinity maximums not exceeding 15 ppt and possibly reaching fresh-water conditions. Between 40 and 52 cm, Ammonia parkinsoniana and Elphidium gunteri are dominant components, indicating salinities as high as 10-15 ppt; Ammotium salsum and Trochammina spp. also are present. In the sample from 30-32 cm, the latter two taxa are dominant, indicating a salinity decrease to <5 ppt. Ostracodes present include cyprids, two non-marine taxa, Candona, and Darwinula, and Perissocytheridea (a genus characteristic of brackish water). This assemblage indicates salinities ranging from <5-10 ppt; the presence of charophytic algae and non-marine ostracodes indicates some fresh-water influence on the site. Pollen assemblages are similar to those in zone II, and an increased concentration of Pediastrum occurs at 30-32 cm.

In zone IV (30-0 cm depth), the agglutinated foraminifers Ammotium salsum and Trochammina spp. dominate the foraminiferal fauna. Ostracodes present include oligohaline-limnic taxa of cyprids, Cyprideis, and Perissocytheridea. These taxa com­ monly are associated with brackish environments with salinities usually exceeding 5 ppt, although further species-level work on ostracode assemblages is warranted. Charophytes

Page 9: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

(fresh-water algae also referred to as stoneworts) are also present in this zone. Pediastrum concentration increased from < 1,000 per gram to >25,000 per gram in this zone, and Botryococcus, another colonial green alga, also occurs for the first time (Fig. 5). Although little is known about the ecology of Pediastrum, increased concentrations have been documented in polluted and nutrient-enriched lakes surrounded by rich macro- phyte vegetation (Digerfeldt, 1977; Fredskild, 1973; Korde, 1961; Parra Barrientos, 1979), and it has been used as an indicator of the trophic status and histories of lakes in Europe (Cronberg, 1982). Less is known about the ecology of Botryococcus', it typically is found in fresh-water bogs, pools, and lakes and may form thick surface scums (Baldwin and Hawker, 1915). Botryococcus proliferates in areas surrounded by scrub plants with nutrient enrichment from humic matter (Dulhunty, 1944). Dinocyst abun­ dance decreased in zone IV, with Polysphaeridium zoharyi becoming nearly absent and Spiniferites sp. the primary taxon present. Pollen concentrations increased up to three­ fold in this interval, but the only notable change in assemblage composition was the slight increase in Liquidambar.

0 10 20 30 0 0 0 0 0 0 0 0 0 0102030405060010203040500 0

Palynomorphs/grarn dry sediment (x103)

Figure 5. Concentration (pollen/gram dry sediment) of pollen of major plant groups, core LP 97-la, Lake Pontchartrain, Louisiana. TCT - Taxodiaceae/Cupressaceae/Taxaceae.

DISCUSSION

Although mixing of the upper meter of sediment by 20th century shell mining pre­ cludes development of an age model for the past few centuries, both the past 50 years and middle Holocene sediments were identified in these cores. These correlations are based on radiocarbon dating, short-lived radioisotopes (137Cs), and biostratigraphic correlation between cores.

Page 10: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

Assemblage Depth Zone (cm)

Salinity (parts per thousand)5 10 15 20

IV

0-2

10-1224-2630-3240-4250-5260-6274-7684-8690-9294-96100-102120-122140-142160-162180-182200-202220-222240-242260-262280-282300-302320-322344-347

Ostracodes Benthic Foraminifera

Cyprids jr AmmotiumCyprideis \ TrochamminaPerissocytheridea 11

Darwinula, Candona, Cyprids

PerissocytherideaCandonaCyprids

Ammotium, Trochammina Ammonia, Elphidium

Ammonia Elphidium

Paracytheroma Cytherura Megacythere Cytheromorpha

Elphidium Ammonia

V

Figure 6. Salinity estimates based on benthic foraminifera and ostracode assemblages, core LP 97-la, Lake Pontchartrain, Louisiana.

Middle Holocene sediments (-5,100 to 4,200 yrBP) are preserved in the interval between 340 and 90 cm in core LP 97-la; these were deposited in a polyhaline (18-25 ppt) bay environment, similar to many bays and lagoons of the modern Gulf coast. During that interval, pollen assemblages indicate few changes in terrestrial vegetation; close modern analogs for down-core assemblages occur along the Mississippi and Ala­ bama portions of the Gulf Coast and along the east coast of the U.S. between northern North Carolina and Key Largo. Summer (July) temperatures in these regions have ranged from 27.1-29.4° C between 1989 and 1998, winter (January) temperatures ranged from 10.0-22° C, and mean annual precipitation ranges from 98-203 cm (NOAA, 1989- 1997a; NOAA, 1989-1997b; NOAA, 1989-1997c; NOAA, 1989-1997d; NOAA, 1989- 1997e).

Faunal changes between 86 cm and 90 cm depth indicate a salinity shift to mesohaline conditions, and herbaceous taxa were more abundant in the pollen flora. In the interval above 120 cm, close modern analogs for pollen assemblages are found only along the Gulf Coast of Mississippi and Alabama and the east coast of southern Florida. Summer temperatures in these areas range from 26.9-28.7° F, winter temperatures range from 10.1 - 22.0° C, similar to those indicated for earlier assemblages. Mean annual precipitation, however, was higher, ranging from 153-203 cm. This is suggestive of greater precipitation during this interval of reduced salinity and is consistent with onset of wetter conditions beginning in the middle Holocene.

The high concentrations of pollen and Pediastrum in the upper 30 cm of LP 97- la are mirrored in core 9-7-96-7; in the latter core, sediments above that interval were deposited

Page 11: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

Table 4. Openings of the Bonnet Carre' spillway (modified from U.S. Army Corps of Engineers, 2001).

Date Duration Max. Flow (cfs)

1937 (1/30-3/7) 211,0001945 (3/23-5/18) 318,0001950 (2/10-3/19) 223,0001973 (4/8-6/21) 195,0001975 (4/14-4/26) 110,0001979 (4/18-5/21) 191,0001983 (5/20-6/23) 268,0001997 (3/17-4/18) 243,000

after 1950, and we suggest that these events are correlative with those in LP 97-la. The increased abundance of both Pediastrum and Botryococcus is consistent with greater influx of fresh water and may indicate increased eutrophication during this interval. This may reflect phytoplankton response to numerous openings of the Bonnet Carre' spillway after 1950 (Table 4). Such openings occurred during flood events of a great enough magnitude that Mississippi River water levels threatened the stability of the main levees in New Orleans and other downstream communities (U.S. Army Corps of Engineers, 2001). Our data indicate that the impacts of spillway opening, including increased sediment and nutrient load, had an impact on phytoplankton assemblages that was un­ precedented during the last 5,500 years. More detailed study of benthic faunas would determine their response to such abrupt changes and the length of time needed to recover to their previous state.

CONCLUSIONS

Phytoplankton assemblages and benthic faunas from three cores collected in Lake Pontchaitrain indicate lower salinity and, possibly, increased eutrophication after 1950 AD. During this time, the Bonnet Carre' spillway was opened six times, releasing Mis­ sissippi River flood waters into the lake, and the floral and faunal changes appear to reflect the biotic response to changes in circulation, sedimentation, nutrient load associ­ ated with these releases. These recent assemblages contrast with those from middle to late Holocene sediments, preserved in core LP 97-la. Older benthic faunas document a gradual change from a polyhaline bay environment to a mesohaline environment. Pollen assemblages indicate a concommitant shift to greater precipitation. It is possible that analysis of faunas and phytoplankton assemblages from less-disturbed nearshore sites or marshes fringing the lake may clarify both how spillway openings affected these commu­ nities and their subsequent recovery from such events.

ACKNOWLEDGEMENTS

We thank Patrick Buchanan, Andrew Fagenholz, Neil Waibel, and Lisa Weimer for

10

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technical support to process samples for analysis of pollen and dinocysts. Neil Waibel also assisted in pollen counts. Sampling assistance was provided by Mike Brown, Jim Flocks, and Phil McCarty. We are grateful to Julie Damon for editorial assistance in assembling the report. We thank Jack Kindinger, Shea Penland, and Paul Conner for assistance in selection of cores for analysis. Jack McGeehin provided sample preparation for AMS dating of samples and calibration of radiocarbon dates. The research was supported by U.S. Geological Survey Coastal and Marine Geology Program.

REFERENCES

Baldwin, M., and Hawker, H.W., 1915, Soil Survey of the Fort Lauderdale Area, Florida:Field Operations of the Bureau of Soils, U.S. Dept. Agric., v. 1915, p. 751-798.

Benninghoff, W.S., 1962, Calculation of pollen and spore density in sediments by addi­ tion of exotic pollen in known quantities: Pollen et Spores, v. 4, p. 332-333.

Cronberg, G., 1982, Pediastrum and Scenedesmus (Chlorococcales) in sediments fromLake Vaxjosjon, Sweden: Arch, fur Hydrobiologie, Suppl., v. 60, no. 4, p. 500-507.

Digerfeldt, G., 1977, Palaeoecological studies of recent development of Lake Vaxjoson.II. Settlement and landscape development: Archives Hydrobiologie, v. 27, p. 131-161.

Dulhunty, J.A., 1944, Origin of New South Wales torbanites: Proceedings of the LinneanSociety of New Southwales, v. 69, p. 26-48.

Edwards, L.E., and Willard, D.A., in press. Dinoflagellate cysts and pollen from sedi­ ment samples, Mississippi Sound and Gulf of Mexico: U.S. Geological SurveyBulletin.

Fredskild, B., 1973, Studies in the vegetational history of Greenland. Palaeobotanicalinvestigations of some Holocene lake and bog deposits: Meddedelser om Gronland, v.198, no. 4, p. 1-245.

Korde, N.W, 1961, Charakteristishce Merkmale der Stratification derBodenablagerungen in Seen rnit verschiedenartigem Zufluss: Verh. Internal. Verein.Limnol., v. 14, no. 524-532.

Litwin, R.J., and Andrle, V.A.S., 1992, Modern palynomorph and weather census datafrom the U.S. Atlantic Coast (Continental Margin Program samples and selectedNOAA weather stations): U.S. Geological Survey Open-file Report, v. 92-263, p. 1-31.

Maher, L.J., Jr., 1981, Statistics for microfossil concentration measurements employingsamples spiked with marker grains: Review of Palaeobotany and Palynology, v. 32, p.153-191.

NOAA, 1989-1997a, Florida: Climatological Data Annual Summary, v. 93-102, no. 13. NOAA, 1989-1997b, Georgia: Climatological Data Annual Summary, v. 93-102, no. 13. NOAA, 1989-1997c, Louisiana: Climatological Data Annual Summary, v. 94-103, no. 13. NOAA, 1989-1997d, Mississippi: Climatological Data Annual Summary, v. 93-102, no.

13. NOAA, 1989-1997e, South Carolina: Climatological Data Annual Summary, v. 92-101,

no. 13. Overpeck, J.T., Webb, T., Ill, and Prentice, I.C., 1985, Quantitative interpretation of fossil

11

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pollen spectra: dissimilarity coefficients and the method of modern analogs: Quater­ nary Research, v. 23, p. 87-108.

Parra Barrientos, O.O., 1979, Revision der Gattung Pediastrum (Meyen) Chlorophyta:Bibliotheca phycologica, v. 48, p. 1-242.

Poag, C.W., 1978, Paired foraminiferal ecophenotypes in Gulf Coast estuaries: ecologi­ cal and paleoecological implications: Transactions of the Gulf Coast Association ofGeological Societies, v. 28, p. 395-421.

Poag, C.W., 1981, Ecological Atlas of Bentic Foraminifera of the gulf of Mexico:Stroudsburg, PA, Hutchinson and Ross, 174 p.

Stockmarr, J., 1971, Tablets with spores used in absolute pollen analysis: Pollen etSpores, v. 8, p. 615-621.

Stockmarr, J., 1973, Determination of spore concentration with an electronic particlecounter: Danmarks Geologiske Unders0gelse, Arbog, v. 1972, p. 87-89.

Stuiver, Minze and Reimer, P.J., 1993, Extended 14C Database and RevisedCALIB Radiocarbon Calibration Program: Radiocarbon, v. 35, p.215-230.

Stuiver, M., Reimer, P.J., Bard, E., Beck, J.W., Burr, G.S., Hughen, K.A.,Kromer, B., McCormac, F.G., v. d. Plicht, J., and Spurk, M., 1998a.INTCAL98 Radiocarbon age calibration 24,000 - 0 cal BP Radiocarbon 40:1041-1083.

U.S. Army Corps of Engineers, 2001, The Bonnet Carre' Spillway, http://www.mvn.usace.army.mil/pao/bcarre/.

12

Page 14: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

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Page 15: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 1.

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ontin

ued

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0)

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2.67

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2.37

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Page 16: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 2.

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Page 17: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 2.

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Page 18: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

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endi

x 3.

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0.51

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Page 19: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 3.

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tinue

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0.26

2.13

1.25

0.63

0.30

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0.32

0.54

0.90

0.34

0.67

0.53

1.00

0.29

1.22

0.67

0.55

0.77

0.61

0.84

1.03

0.66

0.87

Ambrosia

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1.79

0.61

0.93

0.63

0.30

0.00

0.00

2.43

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1.34

0.34

1.06

0.33

0.57

1.52

0.00

1.66

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0.00

0.56

0.00

0.00

0.58

Asteraceae

3.36

3.07

3.65

2.80

1.88

0.89

1.21

0.32

4.59

1.80

2.01

1.01

1.85

1.99

6.32

2.13

4.04

4.14

4.87

4.91

2.53

3.79

2.65

2.03

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9.97

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587.

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12.7

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3.34

11.4

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12.5

615

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5.23

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0.84

3.84

2.74

4.05

2.19

4.75

2.73

5.45

0.54

5.41

4.03

3.37

5.03

1.33

2.01

3.95

6.40

5.80

4.36

5.21

4.21

5.52

4.97

2.33

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0.00

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Page 20: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 3.

Con

tinue

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0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.33

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0.00

0.00

0.00

0.00

0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.26

0.00

0.00

0.00

0.00

0.00

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0.00

0.51

0.00

0.31

0.31

0.00

0.00

0.00

0.27

0.00

0.34

0.00

0.00

0.00

0.00

0.30

0.67

0.28

0.26

0.00

0.56

0.00

0.33

0.00

Myriophyllum

0.00

0.00

0.00

0.00

0.00

0.00

0.30

0.00

0.00

0.00

0.00

0.34

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.34

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0.26

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0.00

0.00

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0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

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3.64

4.09

5.17

3.43

5.64

1.78

3.33

7.37

3.78

6.31

3.69

6.73

4.23

1.99

7.47

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8.84

8.72

6.75

4.78

8.62

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0.28

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0.30

0.00

0.31

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0.00

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0.27

0.60

0.67

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0.26

0.66

0.00

0.00

0.34

0.55

0.26

0.00

0.00

0.00

0.00

0.00

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0.28

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

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0.56

0.77

0.00

0.62

0.31

0.00

0.61

0.32

0.00

0.00

0.34

0.00

0.53

0.33

0.00

0.00

0.00

0.28

0.00

0.61

0.00

0.69

0.33

0.29

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3.34

3.43

5.64

1.78

1.82

1.60

3.51

3.30

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1.35

2.38

4.32

4.89

2.74

1.01

5.25

3.85

2.45

2.53

2.76

4.30

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0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.34

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

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Page 21: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 4.

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Page 22: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 4.

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tinue

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Page 23: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 4.

Con

tinue

d

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236

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Page 24: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 4.

Con

tinue

d

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Page 25: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 5.

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0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.30

0.00

0.32

0.00

0.00

0.00

Page 26: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 5.

Con

tinue

d

.c Q.

<D

Q 0-2

cm4-

6

10-1

2

14-1

6

20-2

2

24-2

6

30-3

2

40-4

2

60-6

2

80-5

2

90-5

2

100-

102

120-

122

140-

142

160-

162

180-

182

200-

202

220-

222

240-

242

280-

282

320-

322

344-

346

^ 0.36

0.61

0.32

1.53

1.22

0.30

0.31

0.00

0.00

0.00

0.26

0.00

0.27

0.51

0.40

0.00

0.00

1.26

0.97

0.31

0.37

0.00

Ostrya/Carpinus

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.31

0.00

0.00

SP 8 a: 0.00

0.00

0.00

0.00

0.00

0.00

0.31

0.00

0.32

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

1 5:67

.38

45.8

749

.20

60.2

4

57.1

4

51.5

1

59.2

6

58.3

8

49.0

4

45.5

7

63.5

2

58.7

0

61.1

9

80.5

6

79.3

5

78.6

6

67.7

5

61.3

2

57.7

9

52.5

0

73.9

0

67.9

1

Quercus

6.81

12.5

4

18.6

510

.70

10.9

4

10.5

4

10.4

9

11.9

8

13.7

8

12.1

3

7.87

12.9

9

12.1

3

6.39

10.9

3

8.28

17.7

5

17.9

2

19.4

8

21.2

5

8.82

10.4

5

.* 3 0.36

0.31

0.96

0.31

0.30

0.60

0.00

0.30

0.32

2.30

0.26

0.26

0.00

0.00

0.00

0.00

0.00

0.31

0.00

0.31

0.00

0.00

e 5.73

3.67

0.96

1.22

1.22

6.33

1.85

2.99

3.85

3.93

3.67

5.19

2.70

2.30

1.62

1.27

2.96

1.57

4.55

4.38

0.37

2.24

:§ is 0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.26

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

1 0.00

1.53

0.64

1.22

0.00

0.00

1.85

0.00

0.64

0.66

0.00

0.26

0.00

0.00

0.00

0.00

0.00

0.31

0.65

0.94

1.10

0.75

Ambrosia

0.00

0.61

0.64

0.92

0.30

1.51

0.93

0.90

0.64

0.98

0.26

0.00

0.00

0.26

0.00

0.64

0.00

0.00

0.00

0.63

0.00

1.49

Asteraceae

2.15

2.75

1.93

1.83

0.91

3.92

1.85

3.59

4.81

3.28

1.84

2.34

1.89

0.26

0.40

0.64

0.89

0.94

2.27

2.50

1.47

3.73

Chenopodiaceae

2.87

12.5

48.

36

4.89

10.9

4

8.13

4.32

8.98

11.2

2

14.4

3

4.99

9.09

7.28

2.05

1.62

1.59

1.18

5.35

3.25

5.31

2.94

2.24

Cladium 0.00

0.00

0.00

0.31

0.00

0.00

0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

Cyperaceae

4.66

4.89

3.54

5.20

5.17

3.61

3.70

1.80

4.17

4.26

3.94

0.78

1.08

1.28

0.81

1.91

1.48

1.89

1.30

1.56

2.57

2.99

Hippocratea

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.26

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.37

0.00

Labiatae

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.27

0.00

0.00

0.32

0.00

0.00

0.00

0.00

0.00

0.00

Leguminosae

0.00

0.00

0.00

0.00

0.30

0.00

0.00

0.30

0.32

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

1 1 0.

0.00

0.00

0.00

0.00

0.00

0.00

0.31

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.31

0.00

0.00

0.00

0.00

Page 27: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 5.

Con

tinue

d

£ Q.

& 0-2

cm4-

6

10-1

2

14-1

6

20-2

224

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30-3

2

40-4

2

60-6

2

80-5

2

90-5

2

100-

102

120-

122

140-

142

160-

162

180-

182

200-

202

220-

222

240-

242

280-

282

320-

322

344-

346

Poaceae

3.23

3.98

3.86

4.59

2.13

5.72

3.09

2.99

3.85

4.59

6.30

3.90

4.04

1.28

2.43

0.96

2.66

3.14

2.92

3.44

1.10

3.73

Polygonaceae

0.00

0.31

0.00

0.00

0.00

0.00

0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

Sagittaria

0.00

0.31

0.64

0.00

0.30

0.00

0.00

0.00

0.00

0.00

0.00

0.26

0.00

0.00

0.00

0.00

0.30

0.00

0.00

0.31

0.00

0.00

1 1.43

3.98

3.54

2.14

2.43

4.22

2.47

1.50

0.96

1.31

0.26

1.30

2.16

0.00

0.00

0.00

0.00

0.31

0.32

0.63

0.37

0.00

Umbelliferae

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.32

0.00

0.00

0.00

Page 28: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

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Depth

TREES AND SHRUBS

Acer

Alnus

Betula

Carya

Celtis

Cephalanthus

Cornus

Corylus

Fagus

Fraxinus

Ilex

Juglans

Liquidambar

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Page 29: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 6.

Con

tinue

d

jr s O 0-2

4-6

10-1

2

14-16

20-2

224

-26

30-3

240

-42

60-6

280

-82

90-9

210

0-10

212

0-12

216

0-16

218

0-18

2

200-202

220-222

240-

242

280-282

320-322

344-346

I 0 0 116

118 0 0 48 0 39 0 0 0 28 0 41 0 0 0 88 0 6

^ 82 101

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197

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1 s:15

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7,574

17,768

23,2

2222,456

13,4

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9,262

5,508

5,894

11,843

7,711

8,897

6,344

17,559

10,124

13,3

7739

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1 1 ,68

2

14,797

7,32

0501

Quercus

1,55

12,070

6,736

4,12

64,

300

2,74

5

1,640

1,13

01,

656

3,153

956

1,96

81,258

2,419

1,066

3,505

11,6

813,938

5,989

874

77

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3 82 50 348

118

119

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606

348

472

478

1,647

289

282

462

1,02

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787

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232

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131

264

109 6

HERBACEOUS TAXA

Ambrosia 0 101

232

354

119

392

145

85 77 256

32 0 0 0 82 0 0 0 176 0 11

Asteraceae

490

454

697

707

358

1,020

289

339

578

852

223

354

196

90 82 175

615

459

705

146

28

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653

2,070

3,019

1,886

4,30

02,

118

675

847

1,34

83,749

605

1,378

755

358

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234

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1,497

291

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Cyperaceae

1,061

808

1,277

2,004

2,031

941

579

169

501

1,108

478

118

112

179

246

292

1,23

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3440

255

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Labiatae 0 0 0 0 0 0 0 0 0 0 0 0 28 0 41 0 0 0 0 0 0

00

Page 30: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 6.

Con

tinue

d

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Q 0-2

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100-

102

120-

122

160-

162

180-

182

200-

202

220-222

240-242

280-282

320-

322

344-

346

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656

1,39

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836

1,49

0482

282

462

1,19

3765

590

419

538

123

526

2,049

591

969

109

28

Polygonaceae 0 50 0 0 0 0 0 28 0 0 0 0 0 0 0 0 0 0 0 0 0

Sagittaria 0 50 232 0 119 0 0 0 0 0 0 39 0 0 0 58 0 0 88 0 0

1 § 327

656

1,27

7825

956

1,09

8386

141

116

341

32 197

224 0 0 0 205

66 176

36 0

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£ o CL I24,169

17,217

37,161

40,314

41 ,09

027,610

15,9

679,943

12,4

80

27,691

12,5

5515,983

10,8

9922,576

13,0

34

20,095

65,5

7920,345

28,625

10,3

06793

DINOCYSTS

Lingulodinium 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 73 0

Spiniferites

245

50 116

118

478

706

289

141

77 341

287

118

168

627

205

701 0 919

352

291

11

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531

226

154

170

414

315

140

985

615

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50

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6

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1,34

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Nematospheropsis 0 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 197 0 73 0

Operculodinium 0 0 0 0 0 0 0 0 0 0 0 0 0 90 0 0 0 0 0 0 0

| ' 5 % n 82 50 0 118 0 0 241 0 154

85 32 79 112

90 41 117 0 131 0 73 0

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grains

82 50 232

354

239 0 820

876

963

682

414

551

168 0 205 0 0 66 88 36 0

Total

Dincysts

408

101

116

354

478

1,02

0

1,06

1367

385

596

765

512

419

1,792

861

2,10

30

2,36

32,642

1,85

739

Page 31: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 6.

Con

tinue

d

1 o 0-2

4-6

10-1

214-16

20-2

224-2

6

30-3

240-4

260-6

280-8

290

-92

100-

102

120-

122

160-

162

180-

182

200-202

220-222

240-

242

280-282

320-322

344-346

GREEN

ALGAE

Botryococcus

1,38

8959

1,161

2,240

3,066

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Pediastrum

17,6

3720,752

29,9

61

35,599

54,229

28,7

081,

785

169

116

256

64 79 0 0 0 58 0 66 0 0 99

Pseudoschizaea 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 237

Page 32: USGS · Cyperaceae, Asteraceae, and Typha (Fig. 4). Pediastrum is a consistent part of the assem blage in this interval, although its concentrations are low (

App

endi

x 7.

Lea

d-21

0 an

d ce

sium

-137

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ivity

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9-7-

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9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

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9-7-

96-7

9-7-

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9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

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9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

9-7-

96-7

Dep

th

(cm

)

0-2

2-4

4-6

6-8

8-10

10-1

2

12-1

4

14-1

6

16-1

8

18-2

0

20-2

2

22-2

4

24-2

6

26-2

8

28-3

0

30-3

2

32-3

4

34-3

6

36-3

8

38-4

0

40-4

2

42-4

4

4446

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48-5

0

50-5

2

52-5

4

54-5

6

Mea

nD

epth

(cm

)

1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43 45 47 49 51 53 55

Dry

Bul

k

Den

sity

(g/c

m3)

0.49

0.54

0.58

0.63

0.71

0.65

0.58

0.54

0.51

0.53

0.56

0.59

0.64

0.58

0.52

0.55

0.65

0.70

0.58

0.77

0.73

0.83

0.88

0.88

0.83

0.82

0.79

0.80

% W

ater

Con

tent

65.0

0

61.6

0

5923

58.4

2

54.7

0

57.8

5

59.8

7

61.8

9

63.7

1

6320

60.4

6

58.8

1

5726

59.8

4

64.1

3

61.5

5

56.6

6

55.6

0

5923

50.7

4

52.1

6

48.9

5

47.7

1

45.6

8

47.3

9

49.4

1

51.3

5

51.2

0

Loss

On

Igni

tion

(% D

ry W

t.)

10.4

6

9.18

8.64

8.66

7.92

9.16

9.70

9.40

1026

9.54

8.73

8.53

8.68

9.52

11.1

1

10.4

3

8.88

827

9.07

7.92

9.18

8.10

7.74

7.48

7.86

8.53

8.35

8.98

Tota

l Pb-

210

Act

ivity

(dpm

/g)

7.47

5.05

3.12

2.68

22

9

2.99

3.18

3.31

3.15

2.97

2.50

2.62

2.64

2.90

3.48

3.19

2.80

2.58

2.72

2.08

2.34

2.12

2.09

2.04

2.17

2.41

22

8

2.36

Tota

l Pb-

210

Err

or

+/-

(dpm

/g)

0.09

0.07

0.05

0.05

0.04

0.05

0.05

0.04

0.04

0.04

0.04

0.04

0.04

0.04

0.05

0.04

0.04

0.03

0.04

0.03

0.04

0.04

0.04

0.03

0.04

0.04

0.04

0.04

Ra-

226

Act

ivity

(dpm

/g)

2.37

2.24

2.41

2.00

2.37

1.95

1.98

1.93

1.92

1.90

2.12

2.05

1.99

2.14

1.92

2.06

2.28

2.19

2.29

2.20

1.85

1.85

2.01

2.04

2.17

2.19

2.07

2.18

Ra-

226

Err

or

+/-

(dpm

/g)

0.31

0.30

027

0.30

0.30

0.14

020

0.17

0.16

0.18

020

0.17

0.17

022

0.16

0.18

0.15

0.18

0.19

0.18

0.12

0.07

0.07

0.07

0.13

0.12

0.08

0.12

Exc

ess

Pb-

210

Act

ivity

(dpm

/g)

4.80

2.54

0.65

0.68

-0.0

8

1.04

120

1.38

123

1.07

0.38

0.57

0.64

0.77

1.56

1.11

0.53

0.39

0.42

-0.1

3

0.49

027

0.06

0.00

0.00

022

021

0.18

Exc

ess

Pb-

210

Err

or

+/-

(dpm

/g)

0.32

0.31

027

0.30

0.30

0.15

020

0.17

0.17

0.18

020

0.17

0.18

022

0.17

0.18

0.16

0.18

0.19

0.19

0.13

0.08

0.06

0.08

0.14

0.13

0.08

0.13

Cs-

137

Act

ivity

(dpm

/g)

0.40

0.34

0.47

0.59

0.53

0.47

0.55

0.58

0.56

0.55

0.50

0.54

0.59

0.66

0.85

0.79

0.65

0.63

0.56

0.54 ** ** ** ** ** ** ** **

Cs-

137

Err

or

+/-

(dpm

/g)

0.12

0.14

0.14

0.16

0.15

0.05

0.09

0.06

0.06

0.06

0.07

0.06

0.06

0.11

0.07

0.07

0.05

0.06

0.07

0.06 -- -- -- -- -- -- -- --