use of agricultural wastes as substrates for bioremediation with trametes versicolor

USE OF AGRICULTURAL WASTES AS SUBSTRATES FOR BIOREMEDIATION

WITH TRAMETES VERSICOLOR

Guillem Llorens-‐Blanch

BioremUAB The main objecCve of the research group is the development of novel biological process to degrade contaminants that are not degraded or very l iKle. LigninolyCc fungi are used in our experiments due to his unspecific enzymaCc system, which allows the degradaCon of a wide range of pollutants.

BioremUAB The main objecCve of the research group is the development of novel biological process to degrade contaminants that are not degraded or very l iKle. LigninolyCc fungi are used in our experiments due to his unspecific enzymaCc system, which allows the degradaCon of a wide range of pollutants.

•  White-‐rot basidiomycetes •  Widely distributed in the environment (lignin

degradaCon) •  Able to growth in submerged cultures. •  Extracellular enzymaCc system with potent red-‐ox

capacity: MnP, LiP and Laccase. •  Intracellular enzymaCc system: Cytochrome P450

T.versicolor in the environment (up) and growth in pellet form (down)

Main characteris3cs of Trametes versicolor

Bioreactor treatment

Liquid matrix

Effluents treatment

Solid matrix

Sludge and soil

treatment

•  SyntheCc dyes • PharmaceuCcals and Personal Care Products (PPCPs) • Endocrine-‐DisrupCng Chemicals (EDCs) • Polycyclic AromaCc Hydrocarbons (PAHs) • Brominated flame retardants • Chlorinated solvents (PCE, TCE)

Bioremedia3on of organic pollutants

Air pulsed fluidized bed

Biopile (up) and bioslurry (down)

Liquid waste

Solid waste

•  Wastewater form the University Vila UAB • Wastewater from the veterinary hospital UAB • Wastewater from a hospital • RejecCon stream from the reverse osmosis process in a sewage treatment plant

•  Sludge from the acCvated sludge of a WWTP • Sludge from the MBR of a WWTP

Project DEGRAPHARMAC

Solid Matrices

Matrices Soil

Sludge

Solid Matrices

Matrices Soil

Sludge

Pollutants

PAH

PPCP

Spiked (Creosote) Spiked Real concentraCons

Biopiles Contaminated sludges or soils are mixed with lignocellulosic materials

Tube Biopiles

Inoculum Grown fungus on the support Sludge/Soil

addiCon Pollutant extracCon

Tray Biopiles




Tube Biopiles



Tray Biopiles



7 days


Tube Biopiles



Tray Biopiles



Addition & Mixing


Tube Biopiles



Tray Biopiles



15 - 40 days


Parameters

Fixed Study

Humidity Humidity

Mixture Mixture

Temperature -‐-‐-‐

pH -‐-‐-‐

Methods

Laccase AcCvity

Ergosterol QuanCficaCon

Naproxen test

CO2 ProducCon

Time (d)

Soil bioremedia3on. Searching supports

7 different materials were tested as supports for soil bioremedia3on

rabbit feedstock

(C2)

wheat straw

pellets (C1)

rabbit feedstock

(C3)

maize stalks (Pn)

wheat straw (Pa)

rice husks (Ar)

pine stardust (Pa)

Time (d)

[U·g

-1 D

W]

Time (d)

[mg·

g-1 D

W]

Ergosterol QuanCficaCon

CO2 producCon Laccase AcCvity Screning based on:

Soil bioremedia3on. Tes3ng the supports Sterile soil Non-‐sterile soil

Soil bioremedia3on. Tes3ng the supports Sterile soil Non-‐sterile soil

ND24 [%]

Ergosterol [mg·∙g-‐1 DW]

Laccase Ac3vity [U·∙g-‐1 DW]

Soil bioremedia3on. Tes3ng the supports

rabbit feedstock (C2)

wheat straw pellets (C1)

rabbit feedstock (C3)

Soil bioremedia3on. Tes3ng the supports

wheat straw pellets (C1)

Soil bioremedia3on. PAH removal

S: Sterile oil S+bac: Sterile soil with bacterial inoculum NS: Non-‐sterile soil

Sludge bioremedia3on. Searching supports

Pallets chips

Olive wood chips

Strain wood chips

3 different materials were tested as supports for sludge bioremedia3on

Screning based on: •  CO2 producCon •  Laccase AcCvity •  Ergosterol QuanCficaciton

DegradaCon (%)

Sludge bioremedia3on. PPCP degrada3on

Cultures Characteris3cs

AbioCc 3g Dry Weight + 1g support

Experimental 3g DW+ 1g support + 0.5mL mycelium

Killed Control 3g DW+ 1g support + 0.5mL mycelium (Heat killed)

•  Dried MBR sludge •  11 days growth cultures were used

to colonize the sludge •  The ability of the fungus to

degrade Hydrochlorothiazide (HZT) was studied

Laccase AcCvity

Sludge bioremedia3on. Op3mizing the bioremedia3on

Group Amount of

support (g)

Amount of

sludge (g)

Moisture (%)

1 3.0 6.0 40

2 2.5 6.5 54

3 2.0 7.0 60

4 1.3 7.7 54

5 0.8 8.2 40

6 1.3 7.7 26

7 2.0 7.0 20

8 2.5 6.5 26

9 2.0 6.0 40

Characteris3cs of the cultures divided into groups

Sludge bioremedia3on. PPCP removal

DegradaCon of PPCP at real concentraCons in sterile sludge with Olive wood chips

THANKS FOR YOUR ATTENTION

use of agricultural wastes as substrates for bioremediation with trametes versicolor

Spiritual