understanding the nu-430 / 435 - nuaire · class ii, type b2 biological safety cabinet / fume hood...

Class II, Type B2 Biological Safety Cabinet / Fume Hood

Understanding the NU-430 / 435

A Guide For

Planning

Installation

Operation

21HEPA [High Efficiency Particulate Air]

filters capture only particulate.

Chemicals, fumes, and vapors will pass

through a HEPA filter and enter the

exhaust system.

100% of the cabinet’s contaminated

airflow passes through the Exhaust

HEPA filter.

60% of the cabinet’s total airflow passes

through the Supply HEPA filter creating

a unidirectional downflow.

40% of the total cabinet’s airflow pass

through the front access opening creating

an air barrier.

NU-430 • UL listed, UL-C listed (115 VAC)

• CE (230 VAC)

• SupplyandExhaustHEPAfilter standardULClassII

• Canusestandard,internal servicevalves

• Canuseultravioletlight

• Mustuselabel“DoNotUseToxic orExplosivesinCabinet”

• NSFListed(115VAC)

NU-435 • UL Classified (115 VAC)

• SupplyHEPAfilterULClassI

• ExhaustHEPAfiltertoUL586

• Noultravioletgermicidallightavailable

• Onlyuseremoteservicevalves

• Nolabelrestrictions

• Availablein115V,60Hz.only

Personnel, Product, and Environmental Protection

Description of Class II, Type B2 Safety Cabinet

TheLabGard NU-430biologicalsafetycabinetisaClassII,TypeB2

TotalExhaustcabinetfeaturingflow-throughconstructionwithno

internalrecirculationofairwithinthecabinet.Requiredairtooperate

thecabinetistakeneitherfromtheroomthroughtheworkaccess

openingorthroughthesupplyairintakeductdirectlyintothesupply

airblower.

TheLabGard NU-435biological/chemicalfumehoodisaClassII,

TypeB2TotalExhaustcabinetfeaturingthesamebasicdesign

parametersastheNU-430.

USE: Type B2 cabinets may be used for

work with volatile toxic chemicals and

radionuclides required as an adjunct to

microbiological studies.*

* Definition from NSF / ANSI 49

Understanding the NU-430 airflow system is critical to the safe operation of the cabinet

TheNU-430biologicalsafetycabinetprovidespersonnel,product,

andenvironmentalprotection.Personnelprotectionisprovidedfor

byhaving40%ofthetotalcabinet’sairflowpassthroughthefront

accessopeningcreatinganairbarrier.Theremaining60%ofthe

cabinet’stotalairflowpassesthroughtheSupplyHEPAfilterand

intotheworkareacreatingunidirectional,turbulence-freedownflow

thatminimizesthepossibilityofcross-contaminationwithinthe

workzone.Thetotalcabinet’sairflowisthenpulledthroughthe

ExhaustHEPAfilterandintotheexhaustsystemprovidingexcellent

environmentalprotection.

21HEPA [High Efficiency Particulate Air]

filters capture only particulate.

Chemicals, fumes, and vapors will pass

through a HEPA filter and enter the

exhaust system.

100% of the cabinet’s contaminated

airflow passes through the Exhaust

HEPA filter.

60% of the cabinet’s total airflow passes

through the Supply HEPA filter creating

a unidirectional downflow.

40% of the total cabinet’s airflow pass

through the front access opening creating

an air barrier.

NU-430 • UL listed, UL-C listed (115 VAC)

• CE (230 VAC)

• SupplyandExhaustHEPAfilter standardULClassII

• Canusestandard,internal servicevalves

• Canuseultravioletlight

• Mustuselabel“DoNotUseToxic orExplosivesinCabinet”

NU-435 • UL Classified (115 VAC)

• SupplyHEPAfilterULClassI

• ExhaustHEPAfiltertoUL586

• Noultravioletgermicidallightavailable

• Onlyuseremoteservicevalves

• Nolabelrestrictions

• Availablein115V,60Hz.only

Personnel, Product, and Environmental Protection

Description of Class II, Type B2 Safety Cabinet

TheLabGard NU-430biologicalsafetycabinetisaClassII,TypeB2

TotalExhaustcabinetfeaturingflow-throughconstructionwithno

internalrecirculationofairwithinthecabinet.Requiredairtooperate

thecabinetistakeneitherfromtheroomthroughtheworkaccess

openingorthroughthesupplyairintakeductdirectlyintothesupply

airblower.

TheLabGard NU-435biological/chemicalfumehoodisaClassII,

TypeB2TotalExhaustcabinetfeaturingthesamebasicdesign

parametersastheNU-430.

USE: Type B2 cabinets may be used for

work with volatile toxic chemicals and

radionuclides required as an adjunct to

microbiological studies.*

* Definition from NSF / ANSI 49

Understanding the NU-430 airflow system is critical to the safe operation of the cabinet

TheNU-430biologicalsafetycabinetprovidespersonnel,product,

andenvironmentalprotection.Personnelprotectionisprovidedfor

byhaving40%ofthetotalcabinet’sairflowpassthroughthefront

accessopeningcreatinganairbarrier.Theremaining60%ofthe

cabinet’stotalairflowpassesthroughtheSupplyHEPAfilterand

intotheworkareacreatingunidirectional,turbulence-freedownflow

thatminimizesthepossibilityofcross-contaminationwithinthe

workzone.Thetotalcabinet’sairflowisthenpulledthroughthe

ExhaustHEPAfilterandintotheexhaustsystemprovidingexcellent

environmentalprotection.

LOCATION: Thebiologicalsafetycabinet

shouldbelocatedoutofthetrafficpattern,

andawayfromroomaircurrentsthat

coulddisruptthecontainmentprovided

bytheworkaccessopeningairbarrier.

Windowsshouldremainedclosedatall

timesandroomventilationairinletsshould

notbedirectedacrossthefrontopening.

Clearanceshouldbeprovidedbehindand

oneachsideofthecabinet.

Both supply air and exhaust air have to be controlled for proper operation of the NU-430

Supplyair,typicallytakendirectlyfromtheroomorcanbetakenfrom

aremotesourceifconditioned(i.e.70°F(21°C)30to50%RH).Ifitis

desirabletotakesupplyairfromaremotesource(i.e.adjacenthallway)

morethan5feet(1.5m)fromthecabinet,itwillbenecessarytoaddan

auxiliaryblowertogainadequateairfow.Itwouldalsobenecessaryto

interlocktheauxiliaryblowertotheinternalsupplyblowerofthecabinet

toavoidpressurizationofthecabinetwhennotinuse.Asupplydamper

mayalsoberequireddependinguponsystemdesignasameansto

interlocktheremotesourcetoagainavoidcabinetpressurization.

MAKE UP AIR: Propersizingoftheexhaust

andmakeupsupplysystemsarecritical

tothesuccessfulinstallationofthecabinet.

Adequateroommakeupairinflowmust

replaceexhaustedair.Airdiffusionrate

nottoexceedvelocityof100LFPM[.51m/s]

tominimizedisruptiveaircurrents.

Supply air is typically taken from the room, but can be supplied from a remote source

Theroomorlaboratorywherethecabinetresidesshouldmeetnecessary

ventilationrequirementsforitsintendeduseasspecifiedinguideline

documents(i.e.ANSI/AIHIZ9.5,USP797).UsetheConcurrentBalance

Value(CBV)forcalculatingHVACrequirements.Allductlosses(i.e.duct

diameter,length,numberofelbows,reductions,etc.)mustbeconsidered

andaddedtothecabinetstaticlossrequirementfortheselectionofthe

exhaustblower.Thecabinetshouldneverbethededicatedexhaustfor

theroomorlaboratory.Thereshouldalwaysbeasecondaryroomexhaust

(couldbetiedtothecabinetexhaust)withitsownVariableAirVolume

(VAV)valvetohandlesystemfluctuationsandbetheprimarydynamic

controlleroftheroomorlaboratory.Thecabinetshouldalwaysremainas

aconstantairvolumedevice.ThecabinetcanbeinterfacedtotheBASfor

blowerfanorrelaycontacts,nightsetbackcontacts,andremoteoverride

cabinetshutdowncontacts.

The exhaust system must provide conditions under which the cabinet was certified to meet its stated performance

The biological safety cabinet will not function properly until the exhaust requirements are met.

NU-430 / 435-400 NU-430 / 435-600

ConcurrentBalanceValue

(CFM/CMH): 829/1409 1221/2075

CertificationExhaustValue

(CFM/CMH): 754/1281 1100/1869

PlantDuctStatic

Pressure(Eng/Metric): 1.7”w.g./43mmw.g. 2.0”w.g./51mmw.g.

[A] Measurestatic

pressureand

requiredexhaust.

To External Exhaust System

Foratypicalconfiguration,supplyairwouldbetakenfromtheroom.The

NuAireTouchLinkcontrolsystemwouldautomaticallyregulatetheinternal

supplyblowerviaadigitaldualthermistorairflowsensorlocatedinthe

downflowairstreamtomonitorandcontroltosetpoint.TheTouchLink

controlsystemautomaticallycompensatesforfilterloading,voltage

variances,andotherenvironmentaleffects.

AConstantAirVolume(CAV)valveordampershouldbeplaceddownstream

ofthecabinettoassureasteadyrateofexhaustflow.Theworkaccess

barrier105±5fpm(.53±.025m/s)ismaintainedbytheCAVvalve.The

CAVvalveinmostcasesiscontrolledbytheBuildingAutomationSystem

(BAS),butalsomaybecontrolledbytheTouchLinkcontrolsystemvia

thedigitaldifferentialvelocitypressureflowgridthatmonitorsexhaust

airvolumeandoptionallycontrolsutilizinganactuatedgas-tightdamper

(i.e.NU-951-012).NOTE:Itisalsorecommendedtouseamanualgas-tight

damper(i.e.NU-940-012)mountedjustabovethecabinetforsealingofthe

exhaustductduringthecabinetdecontaminationprocessifastandard

BAScontrolledCAVisused.

Supplyairistypicallytaken

fromtheroom,butcanbe

remotelysuppliedasshown

ifinterlockedproperly

Note:TheExhaustsystemmustbedesignedtoprovidethestaticpressureand

requiredconcurrentbalancevalueatthelocationindicated[A]above.

LOCATION: Thebiologicalsafetycabinet

shouldbelocatedoutofthetrafficpattern,

andawayfromroomaircurrentsthat

coulddisruptthecontainmentprovided

bytheworkaccessopeningairbarrier.

Windowsshouldremainedclosedatall

timesandroomventilationairinletsshould

notbedirectedacrossthefrontopening.

Clearanceshouldbeprovidedbehindand

oneachsideofthecabinet.

Both supply air and exhaust air have to be controlled for proper operation of the NU-430

Supplyair,typicallytakendirectlyfromtheroomorcanbetakenfrom

aremotesourceifconditioned(i.e.70°F(21°C)30to50%RH).Ifitis

desirabletotakesupplyairfromaremotesource(i.e.adjacenthallway)

morethan5feet(1.5m)fromthecabinet,itwillbenecessarytoaddan

auxiliaryblowertogainadequateairfow.Itwouldalsobenecessaryto

interlocktheauxiliaryblowertotheinternalsupplyblowerofthecabinet

toavoidpressurizationofthecabinetwhennotinuse.Asupplydamper

mayalsoberequireddependinguponsystemdesignasameansto

interlocktheremotesourcetoagainavoidcabinetpressurization.

MAKE UP AIR: Propersizingoftheexhaust

andmakeupsupplysystemsarecritical

tothesuccessfulinstallationofthecabinet.

Adequateroommakeupairinflowmust

replaceexhaustedair.Airdiffusionrate

nottoexceedvelocityof100LFPM[.51m/s]

tominimizedisruptiveaircurrents.

Supply air is typically taken from the room, but can be supplied from a remote source

Theroomorlaboratorywherethecabinetresidesshouldmeetnecessary

ventilationrequirementsforitsintendeduseasspecifiedinguideline

documents(i.e.ANSI/AIHIZ9.5,USP797).UsetheConcurrentBalance

Value(CBV)forcalculatingHVACrequirements.Allductlosses(i.e.duct

diameter,length,numberofelbows,reductions,etc.)mustbeconsidered

andaddedtothecabinetstaticlossrequirementfortheselectionofthe

exhaustblower.Thecabinetshouldneverbethededicatedexhaustfor

theroomorlaboratory.Thereshouldalwaysbeasecondaryroomexhaust

(couldbetiedtothecabinetexhaust)withitsownVariableAirVolume

(VAV)valvetohandlesystemfluctuationsandbetheprimarydynamic

controlleroftheroomorlaboratory.Thecabinetshouldalwaysremainas

aconstantairvolumedevice.ThecabinetcanbeinterfacedtotheBASfor

blowerfanorrelaycontacts,nightsetbackcontacts,andremoteoverride

cabinetshutdowncontacts.

The exhaust system must provide conditions under which the cabinet was certified to meet its stated performance

The biological safety cabinet will not function properly until the exhaust requirements are met.

NU-430 / 435-400 NU-430 / 435-600

ConcurrentBalanceValue

(CFM/CMH): 829/1409 1221/2075

CertificationExhaustValue

(CFM/CMH): 754/1281 1100/1869

PlantDuctStatic

Pressure(Eng/Metric): 1.7”w.g./43mmw.g. 2.0”w.g./51mmw.g.

[A] Measurestatic

pressureand

requiredexhaust.

To External Exhaust System

Foratypicalconfiguration,supplyairwouldbetakenfromtheroom.The

NuAireTouchLinkcontrolsystemwouldautomaticallyregulatetheinternal

supplyblowerviaadigitaldualthermistorairflowsensorlocatedinthe

downflowairstreamtomonitorandcontroltosetpoint.TheTouchLink

controlsystemautomaticallycompensatesforfilterloading,voltage

variances,andotherenvironmentaleffects.

AConstantAirVolume(CAV)valveordampershouldbeplaceddownstream

ofthecabinettoassureasteadyrateofexhaustflow.Theworkaccess

barrier105±5fpm(.53±.025m/s)ismaintainedbytheCAVvalve.The

CAVvalveinmostcasesiscontrolledbytheBuildingAutomationSystem

(BAS),butalsomaybecontrolledbytheTouchLinkcontrolsystemvia

thedigitaldifferentialvelocitypressureflowgridthatmonitorsexhaust

airvolumeandoptionallycontrolsutilizinganactuatedgas-tightdamper

(i.e.NU-951-012).NOTE:Itisalsorecommendedtouseamanualgas-tight

damper(i.e.NU-940-012)mountedjustabovethecabinetforsealingofthe

exhaustductduringthecabinetdecontaminationprocessifastandard

BAScontrolledCAVisused.

Supplyairistypicallytaken

fromtheroom,butcanbe

remotelysuppliedasshown

ifinterlockedproperly

Note:TheExhaustsystemmustbedesignedtoprovidethestaticpressureand

requiredconcurrentbalancevalueatthelocationindicated[A]above.

The TOUCHLINK Electronic Airflow Control System

isamicroprocessormodulethatestablishesandmaintains

anoptimumbalancebetweenthesupplyairsystemand

exhaustairsystemthroughconstantmonitoringofairflow

usingadigitaldualthermistorairflowsensorandadigital

differentialvelocitypressureflowgrid.Thefirstsensor

monitorsdownflowairwithintheworkzone,thepressure

flowgridmonitorsexhaustflow.Theinformationisreceivedbythe

ElectronicControlSystemtocontrolbothdownflowandexhaustflow

[ifoptionalactuatedvalveisinstalled]accordingtocalibratedsetpoints

ascalibratedbytheservicetechnician.Thesystemmaintainsa

completediagnosticfunctionandhasremotecontactsforremote

sensingofvariousfunctions.

Plumbing Services:Serviceballvalveswiththetypeofservice

specifiedbytheremovablebuttononthehandle,arelocatedinthe

workzone.Theserviceballvalvesarenotrecommendedforpressure

over75p.s.i.[5.2BAR].Reducingvalvesshouldbeinstalledexternalto

thecabinetifnecessary.Servicevalvesshouldneverbeusedforoxygen

service.Aspecialneedlevalveforoxygenserviceisrequiredand

available.Externalon/offvalvesarerecommendedforallinstallations.

TOUCHLINK Electronic Airflow Control System

NU-430-400 / 435-400 NU-430-600/ 435-600

ConcurrentBalanceValueCFM/CMH: 829/1409 1221/2075

CertificationExhaustValueCFM/CMH: 754/1281 1100/1869

PlantDuctStaticPressureEng/Metric 1.7”w.g./43mmw.g. 2.0”w.g./51mmw.g.

HeatRejected,BTU,PerHour 474 584

Electrical:Volts,AC,Hz 115,60(230,50/60) 115,60(230,50/60)

Amps:Blower/Lights 5/(3) 7/(4)

Amps:Duplex 3/(3) 3/(3)

Amps:Total 8/(6) 10/(7)

12ft.PowerCord(one) 14GA-3Wire,15A 14GA-3Wire,15A

CratedShippingWeight: 570lbs./259kg. 760lbs./345kg.

NetWeight: 520lbs./236kg. 710lbs./322kg.

Specifications

Note:The NU-430 / 435 must be balanced and certified to meet the performance requirements.

Common Errors

Error Indicator

Cabinet turns on but internal

blower does not work.

High level of noise - over 65 dbA.

Air flows out front of cabinet.

High level of vibration.

Possible Cause

Inadequateexhaust.Makesure

exhaustsystemcanhandleairflow

requirements.Staticpressureand

requiredexhaustmustbemeasured

atconnectionbetweentheNU-430/

435andfacilityexhaustsystem.[A]

Ductdiametertoosmall/

lessthan12”(305mm).

Ductsystemnotsecurelyanchored

tobuilding.

Ductsheetmetalgaugesandseams

arelessthan20gaugeforround

duct,thisisrequiredtopreventduct

collapseduetohighstaticpressure

conditions.

TheSupplyAir,ifintegrally

designed,mustbeinterlocked

withtheinternalfanrelayto

assureproperoperations.

Ductsystemisdirectlyattached

tocabinet.Useaflexiblesoft

connectionbetweenductand

cabinet.[i.e.NU-940-001]

For additional informationconcerningtheplanning,installation,

availableoptions,orcorrectoperationalprocedures,pleasecontact

NuAire.1.800.328.3352 or www.nuaire.com