Circle the correct answer

This quiz is an educational tool intended to assist facilities in evaluating their operators’ understanding ofthe AmpliVue Trichomonas Assay procedure. This quiz is not intended to be used as sole evidence of operatortraining or competency. Facilities are responsible for ensuring the quality of the testing performed by theiroperators. When testing controls or patient specimens, follow the current Package Insert instructions and/orProcedure Card provided on the Quidel website._____________________________________________________________________________________________1. What specimen types have been cleared for use with the assay?

a) Bloodb) Clinician-collected vaginal specimen

_____________________________________________________________________________________________2. Which of the following would be the appropriate way to release the specimen into the Lysis

Buffer tube?a) Swirl the swab rapidly for 5 seconds b) Swirl the swab rapidly for at least 10 seconds

_____________________________________________________________________________________________3. What is the time frame and storage conditions for specimens prior to testing?

a) Up to 2 days at room temperatureb) Up to 72 hours at room temperature

_____________________________________________________________________________________________4. Which of the following would be the proper way of mixing the Dilution Tube after adding the

lysed sample?a) Inversionb) Centrifugation

_____________________________________________________________________________________________5. At what temperature do the Dilution and Reaction Tubes need to be stored?

a) 20°C to 28°Cb) 2°C to 8°C

_____________________________________________________________________________________________6. What is the volume of diluted specimen that needs to be added to the Reaction Tube?

a) 15 µLb) 145 µL

_____________________________________________________________________________________________ 7. Processed clinical samples are stable in Lysis Buffer for how long?

a) 24 hoursb) 72 hours

_____________________________________________________________________________________________ 8. How long should you wait until reading results?

a) 25 minutesb) 10 minutes

_____________________________________________________________________________________________9. How long and at what temperature should the Reaction Tubes be amplified?

a) 64˚C for 25 minutesb) 95˚C for 25 minutes

_____________________________________________________________________________________________10. When do you need to detect the process control (C line)?

a) Only when calling a positive resultb) To call a negative result

c) Urined) Throat

c) Swirl the swab rapidly for 3 secondsd) Dip the swab into the buffer tube once

c) Up to 14 days at 2°C to 8°Cd) Must be tested immediately

c) Vortexd) Does not need to be mixed

c) –20°Cd) –70°C

c) 95˚C for 10 minutesd) 64˚C for 10 minutes

c) To call a positive or negative resultd) To call an invalid result

c) 50 µLd) 125 µL

c) 8 hoursd) 7 days

c) 1 hourd) 2 hours

Trichomonas Quiz

QZM211000EN00.qxp_Layout 1 4/16/15 10:52 AM Page 1

Circle the correct answer

This quiz is an educational tool intended to assist facilities in evaluating their operators’ understanding ofthe AmpliVue Trichomonas Assay procedure. This quiz is not intended to be used as sole evidence of operatortraining or competency. Facilities are responsible for ensuring the quality of the testing performed by theiroperators. When testing controls or patient specimens, follow the current Package Insert instructions and/orProcedure Card provided on the Quidel website._____________________________________________________________________________________________1. What specimen types have been cleared for use with the assay?

a) Bloodb) Clinician-collected vaginal specimen

_____________________________________________________________________________________________2. Which of the following would be the appropriate way to release the specimen into the Lysis

Buffer tube?a) Swirl the swab rapidly for 5 seconds b) Swirl the swab rapidly for at least 10 seconds

_____________________________________________________________________________________________3. What is the time frame and storage conditions for specimens prior to testing?

a) Up to 2 days at room temperatureb) Up to 72 hours at room temperature

_____________________________________________________________________________________________4. Which of the following would be the proper way of mixing the Dilution Tube after adding the

lysed sample?a) Inversionb) Centrifugation

_____________________________________________________________________________________________5. At what temperature do the Dilution and Reaction Tubes need to be stored?

a) 20°C to 28°Cb) 2°C to 8°C

_____________________________________________________________________________________________6. What is the volume of diluted specimen that needs to be added to the Reaction Tube?

a) 15 µLb) 145 µL

_____________________________________________________________________________________________ 7. Processed clinical samples are stable in Lysis Buffer for how long?

a) 24 hoursb) 72 hours

_____________________________________________________________________________________________ 8. How long should you wait until reading results?

a) 25 minutesb) 10 minutes

_____________________________________________________________________________________________9. How long and at what temperature should the Reaction Tubes be amplified?

a) 64˚C for 25 minutesb) 95˚C for 25 minutes

_____________________________________________________________________________________________10. When do you need to detect the process control (C line)?

a) Only when calling a positive resultb) To call a negative result

c) Urined) Throat

c) Swirl the swab rapidly for 3 secondsd) Dip the swab into the buffer tube once

c) Up to 14 days at 2°C to 8°Cd) Must be tested immediately

c) Vortexd) Does not need to be mixed

c) –20°Cd) –70°C

c) 95˚C for 10 minutesd) 64˚C for 10 minutes

c) To call a positive or negative resultd) To call an invalid result

c) 50 µLd) 125 µL

c) 8 hoursd) 7 days

c) 1 hourd) 2 hours

Trichomonas Quiz

QZM211000EN00 (04/15)

QZM211000EN00.qxp_Layout 1 4/16/15 10:52 AM Page 2