the expected molecular weight for gst-nup42 aa 1-382 is 67,144 da
DESCRIPTION
Purification of GST-Nups from E. coli via Ligand-Affinity Chromatography. The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da. WCE. MSS. BSA. MW. MSS. kDa. +IPTG. 5 ul. 1ug. 2ug. 4ug. 50ul. 200. 116. 97. 68. BSA. GST-Nup42 AA 1-382. 44. 31. - PowerPoint PPT PresentationTRANSCRIPT
The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da
200
BSA
116
97
68
44
31
kDa MW
Purification of GST-Nups from E. coli via Ligand-Affinity Chromatography
MSS
WCE
+IPTG 5 ul
MSS
50ul 2ug
BSA
4ug1ug
GST-Nup42 AA 1-382
The expected molecular weight of GFP-Mex67 is 96 kDa
Affinity-Capture of Karyopherins from Yeast Lysates Using GST-Nups as Bait
200
116
9768
44
31
kDa MW
MgCl2 eluates
GST-Nup116 FG domain
GST-Nsp1 K>R
GFP
-Kap
GFP
-Kap
GFP
GFP
-Kap
GFP
-Kap
GFP
SDS eluates
yeast HSS yeast HSS
Kaps
The concentration of protein in the GFP-only HSS cytosol was [ ]
The concentration of protein in the GFP-Kap HSS cytosol was [ ]
[Turn the box green as shown, if your GFP-Kap bound to the FG domain in the BEAD HALO assay]
Nucleoplasm
Cytoplasm
Charting the path that karyopherins take across the nuclear pore complex
ER lumen
Nuclear Pore Complex