NANO-FORENSIC ANALYSIS TO IDENTIFY,

INDIVIDUALIZE, AND EVALUATE EVIDENCE

USING NANOPHASE MATERIALS

AIM

• To prepare Fe2O3 nanoparticles by using simple synthesis

process.

• To study the effectiveness of this synthesis process in

application field.

• To characterize the sample using XRD and UV technique.

INTRODUCTION-Iron oxide

• Iron oxides are chemical compounds composed of iron

and oxygen.

• Iron oxides are widely used as inexpensive, durable

pigments in paints, coatings and colored concretes.

• Colors commonly available are in the “earthy” end of the

yellow/orange/red/brown/black range.

SYNTHESIS METHOD

SYNTHESIS METHOD

• Prepared sodium borohydride solution was added drop wise into prepared

ferrous chloride solution until the black precipitate is obtained.

• Iron nanoparticles gets attracted to the magnetic stirrer and centrifuged to

get the extract.

• And repeatedly washed with acetone to bring the pH to 7.

centrifuge

• Bi-phase separation

• Centrifuged repeatedly with acetone to bring the pH to 7.

• At 2500 rpm.

heating

• Heated to remove moisture completely.

• Heated at 90˚C

• Duration 15-20 min.

extract

Extracted ferrous oxide

nanoparticles

X-ray Diffraction (XRD)

• XRD is a rapid analytical technique primarily used for phase

identification of a crystalline material and can provide

information on unit cell dimensions.

• XRD is a versatile and non-destructive technique.

Bragg's Law: 2dsinθ = nλ

CHARACTERIZATION TECHNIQUE USED

Ultraviolet–visiblespectroscopy

It refers to absorption spectroscopy in the ultraviolet-

visible spectral region.

The absorption visible range directly affects the

perceived color of the

chemicals involved. In this region of the electromagnetic

spectrum, molecules undergo electronic transitions.This technique is complementary to fluorescence

spectroscopyIt is used in analytical chemistry for

the quantitative determination of different analyses, such

as transition metal ions, highly conjugated organic

compounds, and biological macromolecules.

Spectroscopic analysis is commonly carried out in

solutions but solids and gases may also be studied.

reSULTS

ReSULT

Observed

value

Standard

value

Peak

position(θ)

2ɵ structure Crystalline

size (Ǻ)

0.535 0.017 26.973 0.518 15.8

0.669 0.017 30.481 0.652 12.6

0.334 0.017 35.833 0.318 26.3

0.802 0.017 43.341 0.786 10.9

0.535 0.017 56.112 0.518 17.4

0.535 0.017 57.480 0.518 17.5

0.669 0.017 63.133 0.652 14.3

ReSULT

The synthesized ferrous oxide nanoparticle diameter wascalculated using Scherrer calculator.

The average particle size of the sample was found to be 16.4 nmwhich is at 46.27◦ using Scherrer’S formula.

ReSULTObserved

value(β)

Standard

ValUe(π/180)

Peak

position(θ) K λCrystalline

size (Ǻ)

0.535 0.017 26.973 0.9 1.54 x 10-10 17.08

0.669 0.017 30.481 0.9 1.54 x 10-10 14.13

0.334 0.017 35.833 0.9 1.54 x 10-10 30.05

0.802 0.017 43.341 0.9 1.54 x 10-10 13.96

0.535 0.017 56.112 0.9 1.54 x 10-10 27.31

0.535 0.017 57.480 0.9 1.54 x 10-10 28.33

0.669 0.017 63.133 0.9 1.54 x 10-10 26.96

DISCUSSION OF RESULT

Debye-Scherrer formula: D= [(K λ)/ (β*(π/180)* Cos(θ))]

The average particle size of the sample was found to be22.54 nm which is at 46.27◦ using DEBYE-Scherrer’S formula

ReSULt

From this UV-visible spectroscopy patterns analysis, we determined peak intensity, position and crystalline data.

Future work

To continue our process in the field of forensics field.

The advantage of our technique is that it can simultaneously

detect blood and saliva and can spatially locate and differentiate

these body fluid types.

conclusion

•The ferrous oxide nanoparticles was prepared

•After synthesis the pH value of sample was observed as 7.

•The crystal size of the ferrous oxide present in sample

was found be 49.7 nm respectively by using XRD.

•The particle size which was done by XRD was supported

by the XRD Scherer’s formula.

reference

1. Iron Oxides: Structure, Properties and Applications (Editors: Arturo I. Martinez

(Center for Research and Advanced Studies, National Polytechnic

InstituteatSaltillo,Mexico))(link:https://www.novapublishers.com/catalog/product_info.ph

p?products_id=35731)

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reference

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DOI:10.1039;Source: PubMed

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Thank yoU…………!!!