test name – hepatitis c virus rna pcr- quantitative · test name – hepatitis b virus dna pcr-...

Test Name – Hepatitis C virus RNA PCR- Quantitative Cerner code: Hepatitis C Virus PCR (VL)

ITEM

Process

Specimen requirement

• Whole blood in serum separator tube (5 ml) • Whole blood in EDTA tube (4 ml)

Transport temperature

• Transport immediately to CP (Central Processing)in Hamad General Hospital, in cool box with ice pack.

• If blood specimen(s) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 6 hours. Beyond that, separate serum or plasma (centrifugation) and store at -70ºC.

Days test performed 5 times / week Turnaround time 1 week Method Real time polymerase chain reaction (PCR)

Reference values

The quantification range: From15 to 100,000,000 IU/ml < 15 IU/ml below the limit of detection of the assay > 100,000,000 IU/ml above the limit of detection of the assay

Interpretation

Positive: HCV RNA detected

• 15 - 100,000,000 IU/ml • < 15 IU/ml (i.e. viral load below the limit of

detection) Negative: HCV RNA not detected

Rejection criteria

• Patient information on request form does not match with information on the sample tube/container.

• There is no doctor’s stamp. • The request form contains no patient’s name and

Hospital or Qatar ID number. • An inadequate volume of specimen for testing is

received. • The specimen has leaked • Heparinized blood • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory. • Clots detected by receiving bench or by instruments.

Performing Lab Location

Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City(ext#5125).

References 1. Kit inserts: COBAS® AmpliPrep/COBAS® TaqMan® HCV

Quantitative Test, version2.0 (Roche)

Test Name - Hepatitis E Virus (HEV) (Cerner code: HEV PCR) (Lab order only)

ITEM Process Specimen

requirement • Whole blood in serum separator tube (5 ml), or


• Transport immediately to Central Processing in Hamad General Hospital, in cool box with ice pack.


Days test performed Once per week

Turnaround time 1 week Method Quantitative real time polymerase chain reaction

Reference value The quantification range: From188 IU/mL & above. < 188 IU/ml below the limit of detection of the assay

Interpretation

Positive: Hepatitis E Virus (HEV) RNA Detected:

• From 188 IU/ml & above. • < 188 IU/mL (i.e. viral load below the limit of

detection) Negative: HEV Virus RNA Not detected

Rejection criteria

• Patient information on request form does not match

with information on the sample tube/container. • There is no doctor’s stamp. • The request form contains no patient’s name and


received. • Heparinized specimens • The specimen has leaked. • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory



References 1. FTD 67.1 - 32_64 – MANUAL – v4 – 2018_06 EN: Fast Track Diagnostics-HEV RNA PCR

Test Name – Influenza PCR (influenza A & B, and Respiratory syncytial viruses A and B. (Cerner code: Influenza Panel PCR)

ITEM

Process


• Nasopharyngeal swab, nasopharyngeal aspirate, nasal swab, nasal wash, sputum, endotracheal aspirate, Throat swab, Oropharyngeal swab, Broncho alveolar lavage, Bronchial wash: (2ml)

• Place swab in tube containing Virus Transport Medium (UTM)


• Transport immediately to CP (Central Processing) in Hamad General Hospital, in a cool box with ice pack.

• If specimen(s) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.

Days test performed Daily

Turnaround time 2 days Method Multiplex, real time polymerase chain reaction (PCR)

Reference value No quantitative value

Interpretation

• Positive Influenza A : Detected Negative: Influenza A not detected

• Positive: Influenza B detected Negative: Influenza B not detected.

• Positive Respiratory syncytial viruses A and B: Detected

• Positive Respiratory syncytial viruses A and B: Detected Negative Respiratory syncytial viruses A and B: Not Detected

Rejection criteria

• Patient information on request form does not match with

information on the sample tube/container. • There is no doctor’s stamp. • The request form contains no patient’s name and Hospital

or Qatar ID number. • An inadequate volume of specimen for testing is received. • The specimen has leaked. • Using wrong swab e.g.; bacteriological swab with cotton

tip and wooden shaft which is unsuitable for viruses. (The recommended swab is with a synthetic tip such as polyester or Dacron® and an aluminum or plastic shaft).

• Improper storage of specimen before submission to virology laboratory

Performing Lab Virology Laboratory, Dept. of Lab Medicine & Pathology, located

Location on 4th floor of building #21, Hamad Medical City(ext#5125).

Reference

- Cepheid® The Xpert Xpress Flu/RSV Assay is an automated in vitro diagnostic test for the qualitative detection of influenza A, influenza B, and Respiratory syncytial viruses A and B. Kit insert Xpert® Xpress Flu/RSV 5 301-6580, Rev. C June 2018. - FTD 48.1 – 32_64 – MANUAL- v2 – 2018_01 EN: Fast Track Diagnostics.

Test Name: FTD multiplex PCR, Dengue Virus & Chikungunya Virus PCR (Cerner code: Chikungunya Virus PCR)

ITEM

Process


• Whole blood in serum separator tube (5 ml), or • Whole blood in EDTA tube (4 ml)



• If specimen cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours.

• Beyond that, separate serum or plasma (centrifugation) and store at -70ºC.

Days test performed Once weekly.

Turnaround time 1Week.

Method Multiplex, real time polymerase chain reaction (PCR)

Reference value No quantitative value.

Interpretation Positive: Chikungunya Virus RNA detected Negative: Chikungunya Virus RNA not detected

Rejection criteria




received. • Heparinized specimens • The specimen has leaked • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory



References • Package insert: “FTD Dengue/Chik test”, Fast-track

Diagnostics FTD 43 – 32_64 – MANUAL- v5 – 2018_01 EN.

Test Name: FTD multiplex PCR, Dengue Virus & Chikungunya PCR (Cerner code: Dengue Virus PCR)

ITEM

Process





• If specimen cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours.

• Beyond that, separate serum or plasma (centrifugation) and store at -70ºC.

Days test performed Once weekly.

Turnaround time 1Week.


Reference value No quantitative value.

Interpretation Positive: Dengue Virus RNA detected Negative: Dengue Virus RNA not detected

Rejection criteria




received. • Heparinized specimens • The specimen has leaked • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory



References • Package insert: “FTD Dengue/Chik test”, Fast-track

Diagnostics FTD 43 – 32_64 – MANUAL- v5 – 2018_01 EN

Test Name – MERS Coronavirus PCR) (Cerner code: MERS Coronavirus PCR)

ITEM Process


• Preferred specimens are from lower respiratory tract

(sputum, endotracheal aspirate, Broncho alveolar lavage): 2ml

• If lower respiratory tract specimen(s) cannot be collected, send oropharyngeal swab, nasopharyngeal swab, or nasopharyngeal aspirate. Place swab in tube containing Universal Transport Medium (UTM)

• Repeated sampling is recommended if patient fits case definition of MERS-CoV infection





Turnaround time 24 hours Method Real time polymerase chain reaction (PCR)


Interpretation Positive: MERS-CoV RNA detected Negative: MERS-CoV RNA not detected

Rejection criteria








References 1. Kit insert: FTD 50.1-32_64 – MANUAL – v6 – 2018_01 EN. Fast Track Diagnostics, 03/2015 V1.

Test Name – Respiratory Virus PCR (Cerner Code: Respiratory Virus PCR)

ITEM

Process


• Nasopharyngeal swab, nasopharyngeal aspirate, nasal swab, nasal wash, sputum(2ml) , endotracheal aspirate(2ml) , Throat swab, Oropharyngeal swab, Broncho alveolar lavage(2ml) , Bronchial wash: (2ml)

• Place swab in tube containing Universal Transport Medium (UTM)







Interpretation

The panel includes: 1- Viruses: Influenza A, Influenza A (H12009, H3& H1)

Influenza B, Para 1,2,3 & 4, Rhinovirus/ Enterovirus, Coronaviruses types 229E, NL63, OC43, and HKU1, RSV A & B, Human metapneumovirus A/ B, Adenovirus, Human Boca virus.

2- Bacteria: Bordetella pertussis, Chlamydia pneumoniae,

Legionella pneumophila, and Mycoplasma pneumoniae.

Positive: Specific respiratory virus/ (es)/ Bacteria: Detected Negative: Specific respiratory virus(es) / Bacteria Not detected

Rejection criteria








References - Kit insert ePlex Respiratory Pathogen Panel- PI1056 – E -Kit insert FTD 2 – 32_64 – MANUAL- v6 – 2018_01 EN: Fast Track Diagnostics- Respiratory 21

Test Name –Influenza PCR (Cerner code: Influenza Panel PCR)

ITEM

Process


Nasopharyngeal swab, nasopharyngeal aspirate, nasal swab, nasal wash, sputum, endotracheal aspirate, Throat swab, Oropharyngeal swab, Broncho alveolar lavage, Bronchial wash: (2ml)

Place swab in tube containing Universal Transport Medium (UTM)


Transport immediately to CP (Central Processing) in Hamad General Hospital, in a cool box with ice pack.

If specimen(s) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.




Interpretation

Influenza A positive / pH1N1 Not detected: Seasonal influenza A: Positive

Influenza A positive / pH1N1 Detected: Pandemic 2009 H1N1: Positive

Influenza A negative/ pH1N1 Not detected: No influenza A infection

Positive: Influenza B detected Negative: Influenza B not detected

Rejection criteria

Patient information on request form does not match with

information on the sample tube/container. There is no doctor’s stamp. The request form contains no patient’s name and Hospital

or Qatar ID number. An inadequate volume of specimen for testing is received. The specimen has leaked. Using wrong swab e.g.; bacteriological swab with cotton


Improper storage of specimen before submission to virology laboratory



Reference - Cepheid® Xpert Flu Assay, multiplex Real-time PCR assay qualitative detection of Influenza A, Influenza B and 2009 H1N1.- Kit insert 301-2138, Rev. D July 2017.

Test Name – FTD Multiplex PCR Fever & Rash: Parvovirus B19 PCR (Cerner code: Fever and Rach PCR)

ITEM

Process


Whole blood in serum separator tube (5 ml), or Whole blood in EDTA tube (4 ml)Throat swab (in UTM)

or CSF (in plain sterile screw capped container, 2 ml) can also be useful


Transport immediately to CP (Central Processing) in Hamad General Hospital, in cool box with ice pack. If specimen (CSF) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.

If blood specimen(s) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 6 hours. Beyond that, separate serum or plasma (centrifugation) and store at -70ºC.

Days test performed Twice per week, depending on number of specimens received. (For CSF within 24 hours)

Turnaround time CSF: Routinely 24 hours including where submitted as a STAT during weekend & 1 week for other specimens.

Method Multiplex, Real time polymerase chain reaction (PCR), (including Enteroviruses, Human herpesvirus6, Human herpesvirus7, Parvovirus B19, Measles virus).

Reference value No quantification value.

Interpretation Positive: Parvovirus B19 DNA detected Negative: Parvovirus B19 DNA not detected

Rejection criteria



or Qatar ID number. There is gross hemolysis in plasma sample. An inadequate volume of specimen for testing is received. The specimen has leaked (All attempts will be made to

salvage CSF). The specimen is in a wrong container Using wrong swab e.g.; bacteriological swabs with cotton

tips and wooden shafts which are unsuitable for viruses. (The recommended swab is with a synthetic tip such as polyester or Dacron® and an aluminum or plastic shaft).


Bloody clotted CSF Performing Lab

Location Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City (ext#5125).

References -Kit inserts FTD 1.1 - 32_64 - MANUAL – v4 - 2018_01

EN: Fast Track Diagnostics- Fever and Rash test.

Test Name – FTD Multiplex PCR Fever & Rash: Measles PCR (Cerner code: Fever and Rach PCR)

ITEM

Process











Interpretation Positive: Measles RNA detected Negative: Measles RNA not detected

Rejection criteria








Location Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City(ext:5125).

References -Kit inserts FTD 1.1 - 32_64 - MANUAL – v4 - 2018_01

EN: Fast Track Diagnostics- Fever and Rash test.

Test Name – FTD Multiplex PCR Fever & Rash: Human herpesvirus7 (HHV7) (Cerner code: Fever and Rach PCR)

ITEM

Process











Interpretation Positive: HHV7 DNA detected Negative: HHV7 DNA not detected

Rejection criteria









References -Kit inserts FTD 1.1 - 32_64 - MANUAL – v4 - 2018_01 EN: Fast Track Diagnostics- Fever and Rash test.

Test Name – FTD Multiplex PCR Fever & Rash: Human herpesvirus6 (HHV6) (Cerner code: Fever and Rach PCR)

ITEM

Process











Interpretation Positive: HHV6 DNA detected Negative: HHV6 DNA not detected

Rejection criteria









Test Name – FTD Multiplex PCR Fever & Rash; Enterovirus (Cerner code: Fever and Rach PCR)

ITEM

Process


Whole blood in serum separator tube (5 ml), or

Whole blood in EDTA tube (4 ml) Body fluids: CSF (plain sterile screw) Respiratory samples (Swabs, Sputum)


Transport immediately to CP (Central Processing) in Hamad General Hospital, in cool box with ice pack.

If specimen (CSF) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.






Interpretation Positive: Enterovirus RNA detected Negative: Enterovirus RNA not detected

Rejection criteria

Patient information on request form does not match with information on the sample tube/container.

There is no doctor’s stamp. The request form contains no patient’s name and Hospital

or Qatar ID number. An inadequate volume of specimen for testing is received. The specimen has leaked. The specimen is in a wrong container Improper storage of specimen before submission to

virology laboratory Performing Lab

Location Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City(ext#5125).


Test Name - FTD multiplex PCR Viral Meningitis: Human parechoviruses. (Cerner Code: Viral Meningitis PCR)

ITEM Process


CSF (Plain sterile screw capped container, 2 ml). Whole blood in serum separator tube (2 ml), or Whole blood in EDTA tube (2 ml)





Days test performed Daily for CSF, other specimens 5 times/ week.


Method Multiplex real time polymerase chain reaction (PCR) (including HSV1, HSV2, Enteroviruses, Varicella-zoster virus, Human parechoviruses & Mumps virus)

Reference value No quantification value

Interpretation Positive: Human parechoviruses RNA detected Negative Human parechoviruses RNA not detected

Rejection criteria



or Qatar ID number. An inadequate volume of specimen for testing is received. The specimen has leaked (all attempts will be made to

salvage CSF). The specimen is in a wrong container Improper storage of specimen before submission to

virology laboratory Bloody clotted CSF Heparinized specimen



References 1. Kit insert FTD 13 – 32_64 – MANUAL- v7 – 2018_03 EN: Fast Track Diagnostics- Viral Meningitis

Test Name - FTD multiplex PCR Viral Meningitis: Mumps PCR (MEDICOM code: Viral Meningitis)

ITEM Process


CSF (Plain sterile screw capped container, 2 ml). Throat swab is acceptable (in VTM) Whole blood in serum separator tube (2 ml), or Whole blood in EDTA tube (2 ml)



If specimen (CSF) or respiratory samples cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.

If blood specimen(s) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 6 hours. Beyond that, separate serum or plasma (centrifugation) and store at -70ºC





Interpretation Positive: Mumps RNA detected Negative: Mumps RNA not detected

Rejection criteria


There is no doctor’s stamp. The request form contains no patient’s name and

Hospital or Qatar ID number. An inadequate volume of specimen for testing is

received. The specimen has leaked (all attempts will be made to




Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical

City(ext#5125).


Test Name - FTD multiplex PCR Viral Meningitis: Varicella-zoster virus (VZV) PCR (Cerner Code: Viral Meningitis PCR)

ITEM Process









Method Multiplex real time polymerase chain reaction (PCR) (including HSV1, HSV2, Enterovirus, Varicella-zoster virus, Human parechoviruses & Mumps virus)


Interpretation Positive: VZV DNA Detected Negative: VZV DNA Not detected

Rejection criteria



or Qatar ID number. An inadequate volume of specimen for testing is received. The specimen has leaked (all attempts will be made to





References 1. Kit insert FTD 13 – 32_64 – MANUAL- v7 – 2018_03 EN: Fast Track Diagnostics- Viral Meningitis.

Test Name - FTD multiplex PCR Viral Meningitis: Herpes simplex virus2 (HSV2) (Cerner Code: Viral Meningitis PCR)

ITEM Process




Transport immediately to Central Processing in Hamad General Hospital, in cool box with ice pack.







Interpretation Positive: HSV2 DNA detected Negative: HSV2 DNA not detected

Rejection criteria


There is no doctor’s stamp. The request form contains no patient’s name and

Hospital or Qatar ID number. An inadequate volume of specimen for testing is

received. The specimen has leaked (all attempts will be made to





Test Name – FTD multiplex PCR Viral Meningitis: Herpes simplex virus1 (HSV1) (Cerner Code: Viral Meningitis PCR)

ITEM Process


• CSF (Plain sterile screw capped container, 2 ml). • Whole blood in serum separator tube (2 ml), or • Whole blood in EDTA tube (2 ml)


• Transport immediately to CP (Central Processing) in Hamad General Hospital, in cool box with ice pack.

• If specimen (CSF) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.






Interpretation Positive: HSV1 DNA detected Negative: HSV1 DNA not detected

Rejection criteria


• There is no doctor’s stamp. • The request form contains no patient’s name and Hospital

or Qatar ID number. • An inadequate volume of specimen for testing is received. • The specimen has leaked (all attempts will be made to

salvage CSF). • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory • Bloody clotted CSF • Heparinized specimen


Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City (ext#5125).

References 1. Kit insert FTD 13 – 32_64 – MANUAL- v7 – 2018_03 EN, V1: Fast Track Diagnostics- Viral Meningitis.

Test Name – FTD multiplex PCR Viral Meningitis; Enterovirus PCR (Cerner code: Viral Meningitis PCR)

ITEM

Process


• CSF (Plain sterile screw capped container, 2 ml). • Whole blood in serum separator tube (4 ml), or • Whole blood in EDTA tube (4 ml)









Interpretation Positive: Enterovirus RNA detected Negative: Enterovirus RNA not detected

Rejection criteria



or Qatar ID number. • An inadequate volume of specimen for testing is received. • The specimen has leaked (all attempts will be made to

salvage CSF). • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory • Bloody clotted CSF • Heparinized specimen



Reference - Kit insert FTD 13 – 32_64 – MANUAL- v7 – 2018_03 EN: Fast Track Diagnostics- Viral Meningitis.

Test Name - BK Virus PCR (Cerner code: BKV PCR)

ITEM Process


• Whole blood in serum separator tube (5 ml), or • Whole blood in EDTA tube (4 ml) • Urine (plain sterile screw capped container, ≥5ml)



• If specimens (urine) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 24 hours. Beyond that, store at -70ºC.


Days test performed Twice per week

Turnaround time 1 week Method Quantitative real time polymerase chain reaction

Reference value The quantification range: From520 IU/mL & above. < 520 IU/ml below the limit of detection of the assay

Interpretation

Positive: BK Virus DNA Detected:

• From 520 IU/ml & above. • < 520 IU/mL (i.e. viral load below the limit of

detection) Negative: BK Virus DNA Not detected

Rejection criteria




received. • Heparinized specimens • The specimen has leaked. • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory Performing Lab

Location Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical

City(ext#5125).

References 1. FTD 55.1 - 32_64 – MANUAL – v4 – 2018_01 EN: Fast Track Diagnostics-BKV

Test Name: FTD multiplex PCR ACE: Epstein-Barr virus (EBV) PCR (Cerner code: Adeno-CMV-EBV PCR)

ITEM

Process


• EDTA whole blood (4 ml) • Body fluids: CSF (plain sterile screw capped

container 2 ml), Broncho alveolar Lavage (BAL), urine (plain sterile screw capped container ≥5ml), Viral Swab 3 ml.


• Transport immediately to CP (Central Processing in Hamad General Hospital, in cool box with ice pack.






Reference value The quantification range: From500 IU/mL to 5000000 IU/mL < 500 IU/mL below the limit of detection of the assay > 5000000 IU/mL above the limit of detection of the assay

Interpretation

Positive: EBV DNA detected:

• 500-5000000 IU/ml • < 500 IU/ml (i.e. viral load below the limit of

detection). • > 5000000 IU/mL above the limit of detection.

Negative: EBV DNA: Not detected

Rejection criteria




received. • Heparinized specimens • The specimen has leaked (except CSF; all attempts

will be made to salvage it).

• The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory • Bloody clotted CSF


Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City (ext#5125).

References FTD 1.1 - 32_64 - MANUAL – v4 - 2018_01 EN: Fast Track Diagnostics-ACE test Adeno-CMV-EBV

Test Name: FTD multiplex PCR ACE: CMV (Cytomegalovirus) PCR (Cerner code: Adeno-CMV-EBV PCR)

ITEM

Process


• Whole blood in serum separator tube (5 ml), or • Whole blood in EDTA tube (4 ml) • Body fluids: CSF (plain sterile screw capped container 2

ml), Broncho alveolar Lavage (BAL), Bronchial wash and urine (plain sterile screw capped container ≥5ml), Viral Swab 3 ml.








Reference value

The quantification range: From1000 to 5000000 IU/ml < 1000 IU/ml below the limit of detection of the assay > 5000000 IU/mL above the limit of detection of the assay

Interpretation

Positive: CMVDNA detected:

• 1000-5000000 IU/ml • < 1000 IU/ml (i.e. viral load below the limit of

detection) • > 5000000 IU/mL above the limit of detection.

Negative: CMVDNA not detected

Rejection criteria




received. • Heparinized specimens

• The specimen has leaked (except CSF; all attempts will be made to salvage it).

• The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory • Bloody clotted CSF.



References FTD 1.1 - 32_64 - MANUAL – v4 - 2018_01 EN: Fast Track Diagnostics-ACE test Adeno-CMV-EBV

Test Name: FTD multiplex PCR ACE: Adenovirus PCR (Cerner code: Adeno-CMV-EBV PCR)

ITEM

Process


• Whole blood in serum separator tube (5 ml), or • Whole blood in EDTA tube (4 ml) • Body fluids: CSF (plain sterile screw capped container 2

ml), Broncho alveolar Lavage (BAL), Bronchial wash and urine (plain sterile screw capped container ≥5ml), Viral Swab 3 ml.






Turnaround time

CSF: Routinely 24 hours including where submitted as a STAT during weekend & 1 week for other specimens.


Reference value

The quantification range: From 1000 to 10,000,000 Copies/mL < 1000 Copies/ml below the limit of detection of the assay > 10,000,000 IU/mL above the limit of detection of the assay

Interpretation

Positive: Adeno Virus DNA: Detected

• 1000 - 10,000,000 Copies/ml • < 1000 IU/mL (i.e. viral load below the limit of

detection) • > 10,000,000 IU/mL above the limit of detection.

Negative: Adeno Virus DNA :Not detected

Rejection criteria




received.

• Heparinized specimens • The specimen has leaked (except CSF; all attempts

will be made to salvage it). • The specimen is in a wrong container • Improper storage of specimen before submission to

virology laboratory • Bloody clotted CSF.



Reference - FTD 1.1 - 32_64 - MANUAL – v4 - 2018_01 EN: Fast Track Diagnostics-ACE test Adeno-CMV-EBV

Test Name – Test Name – Human immunodeficiency virus-1 RNA PCR Cerner code: HIV PCR (VL)

ITEM

Process


Whole blood in EDTA tube (4 ml)


• Transport immediately to CP (Central Processing)in Hamad General Hospital, in cool box with ice pack.

• If blood specimen(s) cannot be delivered immediately to lab, store in a refrigerator (4 - 8ºC) for no more than 6 hours. Beyond that, separate plasma (centrifugation) and store at -70ºC.

Days test performed 3-4 times /week

Turnaround time 1 week Method Real time polymerase chain reaction (PCR)

Reference values The quantification range: From 20 to 10,000,000 Copies/ml < 20 Copies/ml below the limit of detection of the assay > 10,000,000 IU/ml above the limit of detection of the assay

Interpretation

Positive: HIV-1 RNA detected

• 20 - 10,000,000 Copies/ml < 20 IU/ml (i.e. viral load below the limit of detection)

Negative: HIV-1 RNA not detected

Rejection criteria


• There is no doctor’s stamp. • The request form contains no patient’s name and



virology laboratory • Clots detected by receiving bench or by instruments.



Reference 1. Kit inserts: COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test V2.0 (Roche)

Test Name – Hepatitis B virus DNA PCR- Quantitative (Cerner: Hepatitis B Virus PCR (VL)

ITEM

Process






Days test performed 3-4 times per week, depending on number of specimens received

Turnaround time 1 week Method Real time polymerase chain reaction (PCR)

Reference value

The quantification value: From 20 to 170,000,000 IU/ml < 20 IU/ml below the limit of detection of the assay > 170,000,000 IU/ml above the limit of detection of the assay

Interpretation

Positive: HBV DNA detected

• 20 - 170,000,000 IU/ml • < 20 IU/ml (i.e. viral load below the limit of

detection) Negative: HBV DNA not detected

Rejection criteria





virology laboratory • Clots detected by receiving bench or by instruments.


Virology Laboratory, Dept. of Lab Medicine & Pathology, located on 4th floor of building #21, Hamad Medical City(ext:#5125).

References 1. Kit inserts: COBASP

® PAmpliPrep/ COBASP

® PTaqManP

®P

HBV Test; v2.0 (Roche)

Test Name – Hepatitis C RNA Genotyping (Cerner code: HCV Genotyping PCR)

ITEM

Process


• Whole blood in serum separator tube (4-5 ml), or whole blood in EDTA tube (4 ml)




Days test performed Once/week Turnaround time 2 weeks

Method 1. HCV genotyping by real time PCR assay 2. HCV genotype 2.0 assay (Line probe assay; LiPA)

Reference value None

Interpretation • Identify HCV Genotype 1-6 and subtype a and b of

genotype 1 for samples known to be positive for HCV RNA.

Rejection criteria


with information on the sample tube/container. • There is no doctor’s stamp. • Request form contains no patient’s name and Hospital

or Qatar ID number. • There is gross hemolysis in serum or plasma sample. • Heparinized specimens • An inadequate volume of specimen for testing is

received. • The specimen has leaked. • The specimen is in a wrong container



References 1. Package insert Abbott Real time HCV Genotype II assay kit(2008-2014) 2. Kit inserts 11/2017: The VERSANT HCV Genotype 2.0 Assay (LiPA), SIEMENS.

test name – hepatitis c virus rna pcr- quantitative · test name – hepatitis b virus dna pcr-...

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