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SWEDISH EXCELLENCE IN CHROMATOGRAPHY Kanak HPLC and UHPLC Columns

SWEDISH EXCELLENCE IN CHROMATOGRAPHYKanak HPLC Columns

PRODUCT BROCHURE

OUR COMPANYNanghavi, founded in 2017 is a joint venture between the Swedish public listed company Nanologica and The Sanghavi family in India. The two companies have joined together to manufacture high quality HPLC columns and distribute them to the Indian market.

Nanologica is a Sweden based life science company founded in 2004. It is divided into two divisions; Chromatography and Drug Delivery. It is one of the leading research companies in nanotechnology with several patents and provides exceptional high quality HPLC media and columns for analytical and preparative purposes.

The Sanghavi family is based in Mumbai, India. They have been in the manufacturing business since 1989 and moved into the chromatography business in 2006. They specialise in catering to the dynamic needs of the Indian pharmaceutical industry. The site is 25,000 square feet covered manufacturing area and is GMP certified for cosmetics.

ABOUT KANAK COLUMNSNanghavi is producing a stellar line of HPLC columns developed by Nanologica, with excellent separation power and robustness in low and high pH. Our silica technology NLAB Saga™ has unique proper-ties with sharp pore and particle size distributions, which give a low back pres-sures and high plate numbers. With an extremely strong silica backbone our Kanak columns have a very long life cycle. Our proprietary surface chemistry gives excellent selectivity across a wide range of chemistry needs, with an edge in separating basic molecules.

We take pride in delivering the best of service and support, to match our product quality and performance as the solution provider in the pharmaceutical industry.

Do not compromise in science!

OUR COMPANYNanghavi, founded in 2017 is a joint venture between the Swedish public listed company Nanologica and The Sanghavi family in India. The two companies have joined together to manufacture high quality HPLC columns and distribute them to the Indian market.

Nanologica is a Sweden based life science company founded in 2004. It is divided into two divisions; Chromatography and Drug Delivery. It is one of the leading research companies in nanotechnology with several patents and provides exceptional high quality HPLC media and columns for analytical and preparative purposes.

The Sanghavi family is based in Mumbai, India. They have been in the manufacturing business since 1989 and moved into the chromatography business in 2006. They specialise in catering to the dynamic needs of the Indian pharmaceutical industry. The site is 25,000 square feet covered manufacturing area and is GMP certified for cosmetics.

Nanghavi provides you with world class HPLC and UHPLC columns and a wide-ranging portfolio of bonded phases.

• C18• C18 Plus• C8• C4• Phenyl Hexyl• Phenyl Hexyl Plus• PFP• Cyano

Wide range of chemistries to leverage all type of analytes.

• 2.1 mm• 3.0 mm• 4.6 mm• 10 mm*

Choose column IDs for your application; from MS to analytical to semi-preparative purposes.

8 PHASES

4 COLUMN IDs

• 250 mm• 150 mm• 100 mm• 50 mm

Choose the right length for your needs; resolution power to short analytical times.

4 COLUMN LENGTHS

• 2.6 µm• 3.5 µm• 5 µm• 10 µm*• 13 µm*

Particle sizes made for UHPLC and HPLC purposes.

5 PARTICLE SIZES

* items available on request

Index

From Silica To Columns

Silica Production and Functionalisation

Selectivity Comparison (Tanaka Test)

Column Selection Guide

Kanak C18

Kanak C8

Kanak C4

Kanak Phenyl Hexyl

Kanak PFP

Kanak Cyano

Kanak C18 Plus

Kanak Core

Kanak Core C18 Plus

Kanak Core Phenyl Hexyl Plus

7

8

9

11

12

14

16

17

18

19

20

21

22

24

7Nanghavi

FROM SILICA TO COLUMNSWe have been producing, modifying and coating silica for many years. To deliver the best HPLC columns to customers we have modern equipment and demanding quality control at each of the diff erent production stages at the manufacturing and R&D sites in India and Sweden. Moreover, our long experience and knowledge in silica chemistry guarantees high quality and excellent batch to batch reproducibility.

Production of high purity silica

Excellent chromatographic performance

Controlled particle size and particle size distribution

Variety of bonded phases

7Nanghavi

FROM SILICA TO COLUMNSWe have been producing, modifying and coating silica for many years. To deliver the best HPLC columns to customers we have modern equipment and demanding quality control at each of the diff erent production stages at the manufacturing and R&D sites in India and Sweden. Moreover, our long experience and knowledge in silica chemistry guarantees high quality and excellent batch to batch reproducibility.

Production of high purity silica

Excellent chromatographic performance

Controlled particle size and particle size distribution

Variety of bonded phases

8 Nanghavi

SILICA PRODUCTION AND FUNCTIONALISATIONWe make spherical porous silica particles with controlled pore size, particle size, and par-ticle size distribution resulting in excellent chromatographic properties. The SEM (Scanning Electron Microscope) image below shows perfect spherical shapes and narrow particle size distribution with no fines or crushed particles. The magnified image shows perfectly smooth silica surfaces with no irregularities.

We offer a range of phases with different and complementary chromatographic properties. We perform the functionalisation at our production laboratory using proprietary production protocols to produce densely functionalised and end capped silica particles with low residual silanol activity/acidity. The result is particles with excellent chromatographic performance and exceptional chemical stability.

A summary of bonded phases available is shown below:

Stationary phase Chemical structure End capped USP code

C18C18 Plus

Yes L1

C8 Yes L7

C4 Yes L26

Phenyl HexylPhenyl Hexyl Plus

Yes L11

PFP (Pentafluorophenyl) Yes L43

Cyano Yes L10

9Nanghavi

SELECTIVITY COMPARISON (TANAKA TEST)To fully characterize surface properties of the chromatography media the Tanaka test is used. It is an established method to evaluate interactions between a stationary phase and different kinds of analytes. Depending on the analyte, different properties of the stationary phase, like hydrophobicity, ion exchange capacity, steric selectivity, and hydrogen bonding are evaluated.

The tests and analytes used are summarized below:

Analysis conditions: Mobile phase: 1. CH3OH/H2O = 80/20 (v/v) (HR, HS and SS) 2. CH3OH/H2O = 30/70 (v/v) (HBC) 3. CH3OH/20 mM potassium phosphate buffer = 30/70 (v/v) (IEX at pH 2.7 and 7.6); Flow rate: 1 mL/min; Column temperature: 300C; Detection: UV 210 nm; Injection volume: 5 µL

HR/5 (retention factor) – describes the hydrophobic retention of the stationary phase and is measured using the retention factor of pentylbenzene divided by five (5).

HS (hydrophobicity or hydrophobic selectivity) – is the selectivity factor between alkylbenzenes which differ by one methylene group (pentylbenzene and butylbenzene). It provides measurement of the functional groups density.

SS (steric selectivity) – is the ability of the stationary phase to dis-tinguish between molecules with similar structures and hydrophobi-city, but with different shapes like o-terphenyl and triphenylene. The former has the ability to twist and bend, whereas the latter has rigid and flat structure.

HBC (hydrogen bonding capacity) – provides a measure of the number of available silanol groups and the degree of end capping by measuring the selectivity between phenol and caffeine. A low value indicates that the stationary phase has low number of silanol groups available to hydrogen bond with caffeine, and thereby gives a measure of the HBC of the silica surface.

IEX pH 7.6 (total ion exchange capacity) – is the measure of the total silanol activity on silica surface at pH>7 when the residual silanol groups are deprotonated and the amine is protonated (pKa = 9.3). It is estimated by the selectivity factor between benzylamine and phenol.

IEX pH 2.7 (acidic ion exchange capacity) – is estimated by the selectivity factor between benzylamine and phenol, at pH 2.7. At pH

10 Nanghavi

Since the Tanaka test consist of six independent test runs, the best way to look at the results and compare them is to plot the data in Spider web charts (see below). Each axis starts in the centre of the diagram and belongs to one test.

Selectivity comparison (Tanaka test)

1,50

1,47

1,540,39

0,36

0,10

HR/5

HS

SS

HBC

IEXpH7.6

IEXpH2.7

SveaC18

0,711,37

0,950,32

0,29

0,11

HR/5

HS

SS

HBC

IEXpH7.6

IEXpH2.7

SveaC8

0,361,31

0,710,39

0,36

0,14

HR/5

HS

SS

HBC

IEXpH7.6

IEXpH2.7

SveaC4

0,63 1,33

1,21

0,82

0,37

0,13

HR/5

HS

SS

HBC

IEXpH7.6

IEXpH2.7

SveaPhenylHexyl

0,061,00

1,860,38

0,46

0,19

HR/5

HS

SS

HBC

IEXpH7.6

IEXpH2.7

SveaCyano

0,33 1,24

2,610,55

0,51

0,11

HR/5

HS

SS

HBC

IEXpH7.6

IEXpH2.7

SveaPFP

Kanak C18 Kanak C8

Kanak Phenyl HexylKanak C4

11Nanghavi

COLUMN SELECTION GUIDETo choose an appropriate column for a specific application is a difficult task. Different kinds of functionalisation offer different interaction mechanisms between stationary phase and analyte to fit a wide range of applications. The figure below suggests some guide lines in choosing the right type of bonded phase depending on the interaction between the analyte and the stationary phase.

Cyano C4

PFP

C18

C8

C4

C18

C8

C4

C18

C8

C4

Cyano

Cyano

Cyano

Phenyl Hexyl

Phenyl Hexyl

Phenyl Hexyl

Phenyl Hexyl

PFP

PFP

PFP

C8 C18

INCREASE IN HYDROPHOBICITY

INCREASE IN π-π INTERACTIONS

INCREASE IN ELECTROSTATIC/DIPOLE INTERACTIONS

INCREASE IN HYDROGEN BONDING

12 Nanghavi

Kanak C18

Order Information Kanak C18 Columns

Silica : Type B Silica

Particle Size : 2.6, 3.5, 5 µmSurface Area : 300 m2/g

Pore Size : 110 Å

Pore Volume : 0.85 mL/g

Carbon Load : 19%

Ligand Density : 3.7 µmol/m2

Bonded Phase : Dimethyloctadecylsilane

End-Capping : Yes

USP Code : L1

pH Range : 1-9

• General fi rst choice column

• High hydrophobic retention

• Wide range of analytes

• Excellent peak shape for acids and bases

Kanak C18 is the fi rst-choice LC column for a wide range of analy-tes. The high carbon load ensures high retention and selectivity for compounds with moderate to high lipophilicity. Thorough end-cap-ping together with very low acidity and homogenously distributed

with bases as well as acidic compounds.

Kanak C18 will experience reduced ligand cleavage at low pH due to extremely inert silica and high ligand density, providing excellent

Comparison of peak shapes and retention times of Nortriptyline and Amitriptyline

Column : Kanak 150x4.6 mm C18 5 µmMobile Phase : 20% 25 mM KH2PO4 pH 7.0

80% methanol

Flow Rate : 1 mL/min

Temperature : 30 C

Detection : UV 210 nm

Acetylsalicylic acid and related compounds

Column : Kanak 150x4.6 mm C18 5 µmMobile Phase : 60% 0.3% H3PO4

40% acetonitrile


Temperature : 30 C


Thorough end-capping and low polarity of the silica surface in the Kanak C18 is evi-dent in the peak shape of the anti-depressants, compared to competitor brands. The tailing factor for the salicylic acid is 0.96.

1. 4-hydroxy benzoic acid

2. Acetylsalicylic acid

3. Salicylic acid

1. Nortriptyline

2. Amitriptyline

Column Length

Particle Size Column ID (mm) 50 mm 100 mm 150 mm 250 mm

5 µm 3.0 K-0203052-3050 K-0203052-3100 K-0203052-3150 K-0203052-3250

4.6 K-0203052-5050 K-0203052-5100 K-0203052-5150 K-0203052-5250

3.5 µm 2.1 K-0203032-2050 K-0203032-2100 K-0203032-2150 -

3.0 K-0203032-3050 K-0203032-3100 K-0203032-3150 K-0203032-3250

4.6 K-0203032-5050 K-0203032-5100 K-0203032-5150 K-0203032-5250

2.6 µm 2.1 K-0203262-2050 K-0203262-2100 K-0203262-2150 -

3.0 K-0203262-3050 K-0203262-3100 K-0203262-3150 K-0203262-3250


1. Nortriptyline

2. Amitriptyline

3 4 5 6 7 8 9 10 11 12 13Minutes

Kanak

Competitor A

Competitor B




3. Salicylic acid

0 1 2 3 4 5 6Minutes

Acidic analytes are exhibiting high eciencies and symmetrical peak shapes.

residual silanol groups gives excellent peak shape and eciencies

stability with acidic buers.

12 Nanghavi

Kanak C18




Pore Size : 110 Å


Carbon Load : 19%


Bonded Phase : Dimethyloctadecylsilane

End-Capping : Yes

USP Code : L1

pH Range : 1-9

• General fi rst choice column

• High hydrophobic retention

• Wide range of analytes


Kanak C18 is the fi rst-choice LC column for a wide range of analy-tes. The high carbon load ensures high retention and selectivity for compounds with moderate to high lipophilicity. Thorough end-cap-ping together with very low acidity and homogenously distributed

with bases as well as acidic compounds.

Kanak C18 will experience reduced ligand cleavage at low pH due to extremely inert silica and high ligand density, providing excellent

Comparison of peak shapes and retention times of Nortriptyline and Amitriptyline

Column : Kanak 150x4.6 mm C18 5 µmMobile Phase : 20% 25 mM KH2PO4 pH 7.0

80% methanol


Temperature : 30 C


Acetylsalicylic acid and related compounds

Column : Kanak 150x4.6 mm C18 5 µmMobile Phase : 60% 0.3% H3PO4

40% acetonitrile


Temperature : 30 C


Thorough end-capping and low polarity of the silica surface in the Kanak C18 is evi-dent in the peak shape of the anti-depressants, compared to competitor brands. The tailing factor for the salicylic acid is 0.96.



3. Salicylic acid

1. Nortriptyline

2. Amitriptyline

Column Length


5 µm 3.0 K-0203052-3050 K-0203052-3100 K-0203052-3150 K-0203052-3250

4.6 K-0203052-5050 K-0203052-5100 K-0203052-5150 K-0203052-5250

3.5 µm 2.1 K-0203032-2050 K-0203032-2100 K-0203032-2150 -

3.0 K-0203032-3050 K-0203032-3100 K-0203032-3150 K-0203032-3250

4.6 K-0203032-5050 K-0203032-5100 K-0203032-5150 K-0203032-5250

2.6 µm 2.1 K-0203262-2050 K-0203262-2100 K-0203262-2150 -

3.0 K-0203262-3050 K-0203262-3100 K-0203262-3150 K-0203262-3250


1. Nortriptyline

2. Amitriptyline

3 4 5 6 7 8 9 10 11 12 13Minutes

Kanak

Competitor A

Competitor B




3. Salicylic acid

0 1 2 3 4 5 6Minutes

Acidic analytes are exhibiting high eciencies and symmetrical peak shapes.

residual silanol groups gives excellent peak shape and eciencies

stability with acidic buers.

13Nanghavi

Water soluble vitamins

Column : Kanak 150x4.6 mm C18 5 µmMobile Phase : A 25 mM KH2PO4 pH 3.6

B acetonitrile

Gradient : 5-30% B in 8 min

Flow Rate : 1.5 mL/min

Temperature : 25 0C

Low pH stability test using gradient cycles

Column : Kanak 150x4.6 mm C18 5 µmMobile Phase : A 1% TFA in H2O pH 0.9

B 1% TFA in acetonitrile

Gradient Cycle : 10-90% B in 5 min; 90% B in 2 min 90-10% B in 1 min; 10% B in 2 min


Temperature : 60 0C


Clozapine and related impurities

Column : Kanak 100x4.6 mm 3.5 µmMobile Phase : A 0.1% TFA in H2O

B 0.1% TFA in acetonitrile

Gradient : 10-30% B in 4 min;30-95% B in 4 min; 95% in 2 min


Temperature : 25 0C


Batch to batch reproducibility

Column : Kanak 100x4.6 mm 5 µmMobile Phase : Acetonitrile/H₂O 70/30%


Temperature : 30 0C


Excellent peak shapes and selectivities of these various vitamins.

No change in either effi ciency or retention time for ehylbenzene after running gradient cycles at pH 0.9 and 60 0C for more than 7000 column volumes.

The high effi ciency of Kanak C18 3.5 µm gives an extraordinary resolution profi le over the forced degradation sample of Clozapine

High batch to batch reproducibility for both retention factors and effi ciencies.

1. Ascorbic acid

2. Nicotinic acid

3. Pyridoxamine

4. Nicotinamide

5. Folic acid

6. Cyanocobalamine

7. Ribofl avin

1. Impurity C

2. Clozapine

3. Impurity D

4. Impurity A

5. Impurity B

Analytes:

1. Uracil

2. Toluene

3. Propylbenzene

1. Ascorbic acid

2. Nicotinic acid

3. Pyridoxamine

4. Nicotinamide

5. Folic acid

6. Cyanocobalamine

7. Ribofl avin

0 1 2 3 4 5 6 7 8Minutes


0 1 2 3 4 5 6 7 8 9 10Minutes

1

23

45

4,00

5,00

6,00

7,00

8,00

9,00

10,00

11,00

12,00

0

20

40

60

80

100

120

0 1000 2000 3000 4000 5000 6000 7000

Rete

ntio

ntim

e

%In

itial

effi

cienc

y

Column Volumes

%Initialefficiency

Retentiontime

2. Toluene

3. Propylbenzene

Batch

0 1 2 3 4 5 6 7 8 9 10 11 12 13

Minutes

2 34

67

89

14 Nanghavi

Kanak C8



Pore Size : 110 Å


Carbon Load : 11%


Bonded Phase : Dimethyloctylsilane

End-Capping : Yes

USP Code : L7

pH Range : 1-9

• Similar selectivity for lipophilic compounds as C18

• Lower retention than C18

compared to Kanak C18


An alternative media to Kanak C18 that will give lower retention. Due to the more hydrophilic nature of the bonded phase, ionized acids and especially bases can have better peak shapes and slight-

mixture containing both moderately polar and very hydrophobic compounds.

Comparison of peak shapes and retentiontimes between C8 and C18 for two anti-depressants

Column : Kanak 150x4.6 mm C8 and C18 5 µmMobile Phase

80% methanol


Temperature : 30 C


Water soluble organic acids

Column : Kanak 250x4.6 mm C8 5 µmMobile Phase

3% methanol


Temperature : 25 C


The excellent peak shapes for ionized compounds with Kanak C8 is revealed by analysing anti-depressants.

Even at very hydrophilic elution conditions, excellent peak shapes and selectivities of the water soluble organic acids are obtained.

1. Tartaric acid

2. Malic acid

3. Lactic acid

4. Maleic acid

5. Fumaric acid

1. Nortriptyline

2. Amitriptyline


Column Length


5 µm 3.0 K-0202052-3050 K-0202052-3100 K-0202052-3150 K-0202052-3250

4.6 K-0202052-5050 K-0202052-5100 K-0202052-5150 K-0202052-5250

3.5 µm 2.1 K-0202032-2050 K-0202032-2100 K-0202032-2150 -

3.0 K-0202032-3050 K-0202032-3100 K-0202032-3150 K-0202032-3250

4.6 K-0202032-5050 K-0202032-5100 K-0202032-5150 K-0202032-5250

2.6 µm 2.1 K-0202262-2050 K-0202262-2100 K-0202262-2150 -

3.0 K-0202262-3050 K-0202262-3100 K-0202262-3150 K-0202262-3250


1. Nortriptyline

2. Amitriptyline

3 4 5 6 7 8 9 10 11Minutes

C8 5 µm

C18 5 µm

1 2 3 4 5 6 7 8 9 10

Minutes

12

3

4

5

• Slightly dierent selectivity for ionized acids and bases ly dierent selectivity compared to Kanak C18. Good choice for

14 Nanghavi

Kanak C8



Pore Size : 110 Å


Carbon Load : 11%


Bonded Phase : Dimethyloctylsilane

End-Capping : Yes

USP Code : L7

pH Range : 1-9

• Similar selectivity for lipophilic compounds as C18

• Lower retention than C18

compared to Kanak C18


An alternative media to Kanak C18 that will give lower retention. Due to the more hydrophilic nature of the bonded phase, ionized acids and especially bases can have better peak shapes and slight-

mixture containing both moderately polar and very hydrophobic compounds.

Comparison of peak shapes and retentiontimes between C8 and C18 for two anti-depressants

Column : Kanak 150x4.6 mm C8 and C18 5 µmMobile Phase

80% methanol


Temperature : 30 C


Water soluble organic acids

Column : Kanak 250x4.6 mm C8 5 µmMobile Phase

3% methanol


Temperature : 25 C


The excellent peak shapes for ionized compounds with Kanak C8 is revealed by analysing anti-depressants.

Even at very hydrophilic elution conditions, excellent peak shapes and selectivities of the water soluble organic acids are obtained.

1. Tartaric acid

2. Malic acid

3. Lactic acid

4. Maleic acid

5. Fumaric acid

1. Nortriptyline

2. Amitriptyline


Column Length


5 µm 3.0 K-0202052-3050 K-0202052-3100 K-0202052-3150 K-0202052-3250

4.6 K-0202052-5050 K-0202052-5100 K-0202052-5150 K-0202052-5250

3.5 µm 2.1 K-0202032-2050 K-0202032-2100 K-0202032-2150 -

3.0 K-0202032-3050 K-0202032-3100 K-0202032-3150 K-0202032-3250

4.6 K-0202032-5050 K-0202032-5100 K-0202032-5150 K-0202032-5250

2.6 µm 2.1 K-0202262-2050 K-0202262-2100 K-0202262-2150 -

3.0 K-0202262-3050 K-0202262-3100 K-0202262-3150 K-0202262-3250


1. Nortriptyline

2. Amitriptyline

3 4 5 6 7 8 9 10 11Minutes

C8 5 µm

C18 5 µm

1 2 3 4 5 6 7 8 9 10

Minutes

12

3

4

5

• Slightly dierent selectivity for ionized acids and bases ly dierent selectivity compared to Kanak C18. Good choice for

15Nanghavi

Column : Kanak 150x4.6 mm C8 and Kanak 250x4.6 mm C18 5 µmMobile Phase : A 1% AcOH in H₂O

B 1% AcOH in methanol

Gradient : C8: 45% B in 10 min C18: 60% B in 6 min45-90% B in 10 min 60-80% B in 14 min


Temperature : 30 0C


Clozapine system suitability test

Column : Kanak 150x4.6 mm C8 5 µmMobile Phase : 20% 0.38% Et₃N in H₂O

80% methanol


Temperature : 30 0C


Atorvastatin (Lipitor) system suitability test

Column : Kanak 100x4.6 mm C8 3.5 µmMobile Phase : 58% 50 mM NH₄OAc pH 4.6

30% acetonitrile, 12% THF


Temperature : 30 0C


The reversal of the elution order of 2-nitrophenol and 2-chlorophenol reveals the more hydrophilic nature of the stationary phase of C8 compared to C18.

Nice peak shapes and resolution that exceed the USP specifi cations. The very demanding separation between Atorvastatin and its diastereomeric Impurity B is achieved with Kanak C8 3.5 µm in accordance with USP.

1. Phenol

2. 4-Nitrophenol

3. 2-Nitrophenol

4. 2-Chlorophenol

5. 2,4-Dichlorophenol

1. Impurity C

2. Impurity D

3. Impurity A

4. Clozapine

5. Unknown

1. Impurity A

2. Impurity B

3. Atorvastatin

4. Impurity C

Comparison of the elution order of phenols between C8 and C18 bonded silica


3 5 7 9 11 13 15 17 19 21

Minutes

1

2

3

4

54. 2-Chlorophenol


3 5 7 9 11 13 15 17 19 21Minutes

1

2

3

4 5

1 2 3 4 5 6 7 8Minutes

2 3 4 5Minutes

1

2

3

4

5

Rs3,4=6.3

4 5 6 7 8 9 10 11 12 13 14Minutes

12

3

4

Rs2,3=1.5

16 Nanghavi

Kanak C4



Pore Size : 110 Å


Carbon Load : 7%


Bonded Phase : Dimethylbutylsilane

End-Capping : Yes

USP Code : L26

pH Range : 1-8

• Good media for large peptides and proteins

• Very low retention for lipophilic compounds

• Can be run in HILIC-modeGood choice for extremely lipophilic compounds to reduce analyti-cal time. Excellent starting point for analysing peptide and protein mixtures. For intermediately polar analytes, such as amino acids, Kanak C4 can be run in HILIC-mode.

Excellent peak shapes for Nortriptyline and Amitriptyline

Column : Kanak 150x4.6 mm C8 and C18 5 µmMobile Phase : 20% 25 mM KH₂PO₄ pH 7.0

80% methanol


Temperature : 30 0C


Analysis with a peptide mix on Kanak C4 column

Column : Kanak 150x4.6 mm C4 5 µmMobile Phase : A 0.1% TFA in H₂O

B 0.085% TFA in acetonitrile



Temperature : 30 0C


Short retention times and very nice peak shapes of the basic anti-depressants are obtained on Kanak C4.

Very nice peak shapes a retention times for three diff erent peptides on Kanak C4 bonded phase.

1. 14 AA, pI 4.5

2. 9 AA, pI 11.7

3. 32 AA, pI 11.1

1. Nortriptyline

2. Amitriptyline


Column Length


5 µm 3.0 K-0201052-3050 K-0201052-3100 K-0201052-3150 K-0201052-3250

4.6 K-0201052-5050 K-0201052-5100 K-0201052-5150 K-0201052-5250

3.5 µm 2.1 K-0201032-2050 K-0201032-2100 K-0201032-2150 -

3.0 K-0201032-3050 K-0201032-3100 K-0201032-3150 K-0201032-3250

4.6 K-0201032-5050 K-0201032-5100 K-0201032-5150 K-0201032-5250

2.6 µm 2.1 K-0201262-2050 K-0201262-2100 K-0201262-2150 -

3.0 K-0201262-3050 K-0201262-3100 K-0201262-3150 K-0201262-3250

(AA=# amino acid residues in peptide, pI=isoelectric point)

Short retention times and very nice peak shapes of the basic anti-depressants are

2. Amitriptyline

0 1 2 3 4 5

Minutes

1

2


0 5 10 15 20 25 30 35 40 45 50 55 60

Minutes

17Nanghavi

Kanak Phenyl Hexyl



Pore Size : 110 Å


Carbon Load : 16%


Bonded Phase : Dimethylphenylhexylsilane

End-Capping : Yes

USP Code : L11

pH Range : 2-8

• Slightly lower retention compared to Kanak C8

• Diff erent selectivity compared to Kanak C18 and C8

• Electron rich phenyl ring

• Good choice for mixture of aromatics and/or polar protic analytes

The electron rich phenyl ring and the alkyl chain will give a mixed interaction; π-π and hydrophobic interaction, respectively. The π-π interaction is most pronounced with electron defi cient aromatics and protic moieties, such as hydroxyl groups. Good choice as an orthogonal column compared to Kanak C18/C8 in method deve-lopment, where the traditional alkyl-based stationary phases fail to provide adequate separation.

This media can successfully be used in highly aqueous conditions (100 % wettability), providing an excellent media for very polar compounds.

Column : Kanak 150x4.6 mm Phenyl Hexyl and 250x4.6 mm C18 5 µmMobile Phase : A 1% AcOH in H₂O

B 1% AcOH in methanol

Gradients : PhHex: 45% B in 10 min C18: 60% B in 6 min45-90% B in 10 min 60-80% B in 14 min


Temperature : 30 0C


The selectivity diff erence between the Phenyl Hexyl and C18 column can be seen in the exemplifi ed analyses of the phenols above.

1. Phenol

2. 4-Nitrophenol

3. 2-Chlorophenol

4. 2-Nitrophenol


Order Information Kanak Phenyl Hexyl Columns

Column Length


5 µm 3.0 K-0204052-3050 K-0204052-3100 K-0204052-3150 K-0204052-3250

4.6 K-0204052-5050 K-0204052-5100 K-0204052-5150 K-0204052-5250

3.5 µm 2.1 K-0204032-2050 K-0204032-2100 K-0204032-2150 -

3.0 K-0204032-3050 K-0204032-3100 K-0204032-3150 K-0204032-3250

4.6 K-0204032-5050 K-0204032-5100 K-0204032-5150 K-0204032-5250

2.6 µm 2.1 K-0204262-2050 K-0204262-2100 K-0204262-2150 -

3.0 K-0204262-3050 K-0204262-3100 K-0204262-3150 K-0204262-3250

Analyses of various phenols on Phenyl Hexyl and C18 bonded silica

Temperature : 30 0C


0 2 4 6 8 10 12 14 16 18 20Minutes

13

2

4

5

0 2 4 6 8 10 12 14 16 18 20Minutes

1 3

4

5

2

18 Nanghavi

Kanak PFP



Pore Size : 110 Å


Carbon Load : 7%


Bonded Phase : Dimethylpentafl uorophenylpropylsilane

End-Capping : Yes

USP Code : L43

pH Range : 2-8

• Strong retention of protic compounds and analytes with high dipole moments

• Strong π-interaction with electron rich phenyl rings• Outstanding shape selectivity

Due to the highly electron defi cient nature of the aromatic rings of Kanak PFP, the stationary phase will interact strongly with analytes containing polar aprotic and electron rich aromatic moieties. This contrasts with the Kanak Phenyl Hexyl stationary phase.

Additionally, the electronegative fl uorines of the pentafl uorophenyl group will provide strong hydrogen bonding with analytes with protic moieties, such as hydroxyl groups and carboxylic acids. Furthermore, the delocalized charge over the fl uorine-carbon bond will interact with analytes containing dipole moments. Thus, Kanak PFP will give diff erent selectivity compared to Kanak Phenyl Hexyl.

The polar nature of Kanak PFP ensures a fully wettable stationary phase, enabling Kanak PFP to analyse very polar compounds.

Comparison between PFP and C18 bonded phases

Column : Kanak 150x4.6 mm PFP and C18 5 µmMobile Phase : 70% H₂O

30% methanol


Temperature : 30 0C


Comparison between PFP and C18 bonded phases

Column : Kanak 150x4.6 mm PFP and C18 5 µmMobile Phase : 60% H₂O

40% methanol


Temperature : 20 0C


The strong hydrogen bonding of the PFP column is clear from the above comparison with C18. The hydrogen bonding acceptor, caff eine, and the donor, phenol, has much higher retention than for the C18.

The Kanak PFP bonded phase can separate the three cresol isomers, while the corresponding C18 cannot.

1. p-Cresol

2. m-Cresol

3. o-Cresol

1. Caff eine

2. Phenol

Order Information Kanak PFP Columns

Column Length


5 µm 3.0 K-0206052-3050 K-0206052-3100 K-0206052-3150 K-0206052-3250

4.6 K-0206052-5050 K-0206052-5100 K-0206052-5150 K-0206052-5250

3.5 µm 2.1 K-0206032-2050 K-0206032-2100 K-0206032-2150 -

3.0 K-0206032-3050 K-0206032-3100 K-0206032-3150 K-0206032-3250

4.6 K-0206032-5050 K-0206032-5100 K-0206032-5150 K-0206032-5250

2.6 µm 2.1 K-0206262-2050 K-0206262-2100 K-0206262-2150 -

3.0 K-0206262-3050 K-0206262-3100 K-0206262-3150 K-0206262-3250

0 1 2 3 4 5 6 7 8 9 10 11 12Minutes

PFP

C18

1

1

2

2

Detection : UV 210 nm 2. m-Cresol

3. o-Cresol

0 2 4 6 8 10 12 14 16 18 20Minutes

PFPC18

19Nanghavi

Kanak Cyano



Pore Size : 110 Å


Carbon Load : 6%


Bonded Phase : 3-Cyanopropyldimethylsilane

End-Capping : Yes

USP Code : L10

pH Range : 2-7.5

• Very polar stationary phase

• Strong dipole-dipole interactions

• Orthogonal phase in RPLC method development

• Good choice for HILIC and NP

A good choice for analytes having too high retention on an alkyl-based stationary phase, as well as mixtures of very polar and lipophilic analytes. The nitrile group of the stationary phase interacts favourably with analytes containing double and/or triple bonds, thereby making Kanak Cyano optimal for unsaturated compounds.

Due to the very polar nature of the Kanak Cyano bonded phase, it can be used in both HILIC-mode as well as in normal phase chromatography.

Separation of two isomeric polar organic acids

Column : Kanak 150x4.6 mm CN 5 µmMobile Phase : 97% 20 mM NH₄OAc pH 3.9

3% methanol


Temperature : 40 0C


Benzylamine analysed on Cyano bonded silica

Column : Kanak 150x4.6 mm CN 5 µmMobile Phase : 70% 20 mM KH₂PO₄ pH 7.6

30% methanol


Temperature : 30 0C


Very short analytical time and base line separation between the two water-soluble cis/trans-isomeric acids.

Kanak Cyano bonded silica shows very nice peak shape of the basic and very polar benzylamine.

1. Benzylamine

2. Phenol1. Maleic acid

2. Fumaric acid

Order Information Kanak Cyano Columns

Column Length


5 µm 3.0 K-0207052-3050 K-0207052-3100 K-0207052-3150 K-0207052-3250

4.6 K-0207052-5050 K-0207052-5100 K-0207052-5150 K-0207052-5250

3.5 µm 2.1 K-0207032-2050 K-0207032-2100 K-0207032-2150 -

3.0 K-0207032-3050 K-0207032-3100 K-0207032-3150 K-0207032-3250

4.6 K-0207032-5050 K-0207032-5100 K-0207032-5150 K-0207032-5250

2.6 µm 2.1 K-0207262-2050 K-0207262-2100 K-0207262-2150 -

3.0 K-0207262-3050 K-0207262-3100 K-0207262-3150 K-0207262-3250

2. Fumaric acid

0 1 2 3 4 5 6

Minutes

2. Phenol

0 1 2 3 4 5 6 7 8 9 10Minutes

20 Nanghavi

Kanak C18 Plus



Pore Size : 110 Å


Carbon Load : 19%


Bonded Phase : Octadecyl silane

End-Capping : Yes

USP Code : L1

pH Range : 1-10

• Excellent stability

• Proprietary coating ensures solely hydrophobic interaction

• Unparalleled peak shape for ionisable compound

• Low bleeding

Kanak C18 Plus is coated with a proprietary bonding technology, which ensures a fully covered silica surface. The column material is thereby protected against hydrolysis of the ligands at low pH and silica dissolution at high pH. Furthermore, the complete coating will give only hydrophobic interactions, which will give unparalle-led peak shape for all types of analytes.

The proprietary bonding technology binds the ligands strongly, providing exceptionally low bleeding.

Excellent peak shapes for Nortriptyline and Amitriptyline

Column : Kanak 250x4.6 mm C18 Plus 5 µmMobile Phase : 20% 25 mM KH₂PO₄ pH 7.0

80% methanol


Temperature : 30 0C


High pH stability test using gradient cycles

Column : Kanak 250x4.6 mm C18 Plus 5 µmMobile Phase : A 10 mM NH₄HCO₄ pH 10.5

B acetonitrile


Gradient Cycle : 10-90% B in 13 min; 90% B in 5 min;90-10% B in 2 min; 10% B in 5 min

Very high effi ciencies and symmetric peak shapes for Nortriptyline and Amitriptyline. Tailing factor for Nortriptyline is 1.08, the one for Amitriptyline is 1.02

The effi ciencies of the neutral (Progesterone) and basic (Amitriptyline) compound are almost unaff ected during the exemplifi ed stability test after more than 7000 column volumes.

1. Nortriptyline

2. Amitriptyline

Order Information Kanak C18 Plus Columns

Column Length


5 µm 3.0 K-0403052-3050 K-0403052-3100 K-0403052-3150 K-0403052-3250

4.6 K-0403052-5050 K-0403052-5100 K-0403052-5150 K-0403052-5250

3.5 µm 2.1 K-0403032-2050 K-0403032-2100 K-0403032-2150 -

3.0 K-0403032-3050 K-0403032-3100 K-0403032-3150 K-0403032-3250

4.6 K-0403032-5050 K-0403032-5100 K-0403032-5150 K-0403032-5250

2.6 µm 2.1 K-0403262-2050 K-0403262-2100 K-0403262-2150 -

3.0 K-0403262-3050 K-0403262-3100 K-0403262-3150 K-0403262-3250


Temperature : 60 0C


4 6 8 10 12 14 16 18 20 22 24

Minutes

1

2

The effi ciencies of the neutral (Progesterone) and basic (Amitriptyline) compound are

0

20

40

60

80

100

0 1000 2000 3000 4000 5000 6000 7000

%ofinitiale

fficie

ncy

Column Volumes

Amitriptyline

Progesterone

21Nanghavi

Kanak Core

KANAK CORE Kanak Core is a core shell product which consist of a solid core particle coated with a layer of porous silica, as illustrated below. A key feature of core shell columns is the much narrower particle size distribution compared to the fully porous materials for a comparable particle size so that the space among particles in the column reduces and efficiency increases. Usually, columns packed with 3 μm core shell particles produce efficiencies approaching those packed with 2 μm fully porous particles, but at significantly lower back pressures.

Kanak Core offers:

• Exceptional peak shape for basic, acidic and chelating compounds

• High stability at low and high pH (1-10)

• Excellent back pressure profile

2.6 µm

1.6 µm

0.5 µm

Porous layer

Core

22 Nanghavi

Kanak Core C18 Plus



Pore Size : 110 Å


Carbon Load : 7%



End-Capping : Proprietary

USP Code : L1

pH Range : 1.5-10

and low back pressures

• Same bonding as Kanak C18 Plus


The superfi cially porous silica layer is bonded with the same proprietary chemistry as Kanak C18 Plus, providing outstanding peak shapes for ionisable compounds and similar selectivity for all types of analytes. The core shell technology of Kanak Core C18 2.6

ning the back pressure of a 3 µm particle, making it possible to run shorter columns on an ordinary HPLC (30-100 mm length).

Comparison between Kanak Core C18 Plus 2.6 µm and a competitor core shell C18 2.6 µm column

Column : 150x4.6 mm

Mobile PhaseB methanol

Gradient : 10% B in 2 min; 10-90% B in 4 min; 90% B in 3 min


Temperature : Ambient

Detection : MS/MS


Column : 150x4.6 mm





Detection : MS/MS

The nice peak shapes from the Kanak Core column give better quantifi cations of the compounds of interest.

Data kindly provided by Animal, Plant and Food Inspection Center – APFICJiangsu Entry-Exit Inspection and Quarantine Bureau of People’s Republic of China

The Kanak Core column is exhibiting superior chromatographical behaviour compared to the competitor core shell column.


1. Tetramycin

2. Doxycycline Nalidixic acidFlow Rate : 0.35 mL/min


Detection : MS/MS

2. Doxycycline

0 1 2 3 4 5 6 7 8 9

Minutes

Kanak

Competitor 1

1

2

2



Detection : MS/MS

Nalidixic acid

0 1 2 3 4 5 6 7 8 9 10 11 12

Minutes

KanakCompetitor

µm provide similar e¢ciencies as sub 2 µm particles while retai-

• Core shell technology leads to high e¢ciencies

22 Nanghavi

Kanak Core C18 Plus



Pore Size : 110 Å


Carbon Load : 7%




USP Code : L1

pH Range : 1.5-10

and low back pressures

• Same bonding as Kanak C18 Plus


The superfi cially porous silica layer is bonded with the same proprietary chemistry as Kanak C18 Plus, providing outstanding peak shapes for ionisable compounds and similar selectivity for all types of analytes. The core shell technology of Kanak Core C18 2.6

ning the back pressure of a 3 µm particle, making it possible to run shorter columns on an ordinary HPLC (30-100 mm length).


Column : 150x4.6 mm





Detection : MS/MS


Column : 150x4.6 mm





Detection : MS/MS

The nice peak shapes from the Kanak Core column give better quantifi cations of the compounds of interest.


The Kanak Core column is exhibiting superior chromatographical behaviour compared to the competitor core shell column.


1. Tetramycin

2. Doxycycline Nalidixic acidFlow Rate : 0.35 mL/min


Detection : MS/MS

2. Doxycycline

0 1 2 3 4 5 6 7 8 9

Minutes

Kanak

Competitor 1

1

2

2



Detection : MS/MS

Nalidixic acid

0 1 2 3 4 5 6 7 8 9 10 11 12

Minutes

KanakCompetitor

µm provide similar e¢ciencies as sub 2 µm particles while retai-

• Core shell technology leads to high e¢ciencies

23Nanghavi

β-Blockers

Column : Kanak Core 100x4.6 mm C18 Plus

Mobile Phase : 50% 25 mM KH₂PO₄ pH 7.0 50% methanol


Temperature : 40 0C


Cis/trans-isomers of 9-octadecenoic acid

Column : Kanak Core 150x4.6 mm C18 Plus

Mobile Phase : 10% 0.5% Formic acid in H₂O 90% acetonitrile


Temperature : 40 0C


The complete coating of the Kanak Core column is clearly revealed by the excellent peak shapes from these highly basic compounds.

Base-line separation between the cis/trans-isomeric acids.

Order Information Kanak Core C18 Plus Columns

Column Length


2.6 µm 2.1 K-0303262-2050 K-0303262-2100 K-0303262-2150 -

3.0 K-0303262-3050 K-0303262-3100 K-0303262-3150 K-0303262-3250

4.6 K-0303262-5050 K-0303262-5100 K-0303262-5150 K-0303262-5250

1. Oleic acid

2. Elaidic acid

1. Atenolol

2. Metoprolol

3. Timolol

4. Pronanolol

2. Elaidic acid

0 1 2 3 4 5 6 7Minutes

The complete coating of the Kanak Core column is clearly revealed by the excellent

0 1 2 3 4 5 6 7 8Minutes

1 2

3

4

24 Nanghavi

Kanak Core Phenyl Hexyl Plus



Pore Size : 110 Å


Carbon Load : 5%


Bonded Phase : Phenyl hexyl silane


USP Code : L11

pH Range : 1.5-9

• Same proprietary bonding technology as Kanak Core C18 Plus

• An orthogonal column in terms of selectivity as Kanak Core C18 Plus

• Lower hydrophobicity than Kanak Core C18 Plus

Similar chromatographical performance as Kanak Phenyl Hexyl. The electron rich phenyl ring will interact with aromatics and protic moieties, such as hydroxyl groups, through its π-system. Good choice as an orthogonal column compared to Kanak Core C18 Plus in method development, where C18 based stationary phases fail to provide an adequate separation.

Components of explosives

Column : Kanak Core 100x4.6 mm Phenyl Hexyl Plus

Mobile Phase : 60% water 40% acetonitrile


Temperature : 40 0C


Calcium antagonists

Column : Kanak Core 100x4.6 mm Phenyl Hexyl Plus

Mobile Phase : 30% 10 mM KH₂PO₄ pH 6.8 70% methanol


Temperature : 40 0C


The Kanak Core Phenyl Hexyl gives very nice separations between these structurally similar aromatic compounds.

Excellent chromatografi cal performance is exhibited by the Kanak Core Phenyl Hexyl column.

Order Information Kanak Core Phenyl Hexyl Plus Columns

Column Length


2.6 µm 2.1 K-0304262-2050 K-0304262-2100 K-0304262-2150 -

3.0 K-0304262-3050 K-0304262-3100 K-0304262-3150 K-0303262-3250

4.6 K-0304262-5050 K-0304262-5100 K-0304262-5150 K-0303262-5250

1. 1,3-Dinitrobenzene

2. 1,3,5-Trinitrobenzene

3. 2,4-Dinitro toluene

4. 2,4,6-Trinitrotoluene

1. Nifedipine

2. Nitrendipine

3. Diltiazem

4. Verapamil

5. Nicardipine

The Kanak Core Phenyl Hexyl gives very nice separations between these structurally similar aromatic compounds.

1. 1,3-Dinitrobenzene

2. 1,3,5-Trinitrobenzene

3. 2,4-Dinitro toluene

4. 2,4,6-Trinitrotoluene

0 1 2 3 4 5 6 7 8Minutes

1

2 3

4

Detection : UV 230 nm2. Nitrendipine

3. Diltiazem

4. Verapamil

5. Nicardipine

0 2 4 6 8 10 12 14Minutes

1

2

3

4

5

25Nanghavi

CONTACT DETAILS AND ORDER INFORMATIONFor company information, technical support, and order inquires:

Mr. Kshitij Shah

+91-9833772296 | [email protected] | www.nanghavi.com

M1 Kalpita Enclave,

Sahar Road,

Andheri (East),

Mumbai-400069.

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