Supporting Information

for Proteomics

DOI 10.1002/pmic.200700561

Stuart J. Cordwell, Alice C. L. Len, Rachel G. Touma,

Nichollas E. Scott, Linda Falconer, David Jones, Angela Connolly,

Ben Crossett and Steven P. Djordjevic

Identification of membrane-associated proteins from

Campylobacter jejuni strains using complementary

proteomics technologies

ª 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.com

C. jejuni CELLS

MEMBRANE PROTEIN-ENRICHED FRACTION

Sodium carbonate precipitation and ultracentrifugation

C. jejuni MEMBRANE PROTEIN / PEPTIDE MIXTURE

Trypsin digestion

82 PEPTIDE FRACTIONS

Strong Cation Exchange (SCX) + offline fraction collection

~ 32,000 MS/MS SPECTRA GENERATED

Automated Reverse-Phase (RP) chromatography followed by tandem MS (Q-STAR)

IDENTIFICATION OF 2301 UNIQUE PEPTIDES

Automated data processing and database interrogation

432 IDENTIFIED PROTEINS

Supplementary Figure 1

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Supplementary Figure 2

376 proteins56 proteins

21 proteins

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Supplementary Figure 3

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Supplementary Figure 4

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Supplementary Figure 5

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Supplementary Figure 6

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Outer Membrane12%

Inner Membrane8%

Integral Membrane1%

Lipoproteins8%

Periplasmic20%

Flagellum8%

Ribosomal 7%

Cytoplasmic29%

Unknown (with TMR)3%

Unknown (without TMR)4% Outer Membrane

2% Inner Membrane8%

Integral Membrane16%

Lipoproteins4%

Periplasmic7%

Flagellum3%

Ribosomal 8%

Cytoplasmic41%

Unknown (with TMR)8%

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Legends to Supplementary Figures

Supplementary Figure 1. Analysis of the C. jejuni JHH1 membrane protein-enriched

fraction using 2-DLC/MS-MS following tryptic digest.

Supplementary Figure 2. Number of peptides per identified protein following 2-

DLC/MS-MS analysis of the C. jejuni JHH1 membrane protein-enriched fraction.

Manual interpretation of low scoring and single peptide ‘hits’ reduced the number of

confidently identified proteins with only one peptide match.

Supplementary Figure 3. Analysis of identified C. jejuni JHH1 proteins from 2-DE/MS

and 2-DLC/MS-MS datasets.

Supplementary Figure 4. Comparison of identified C. jejuni JHH1 proteins from (A) 2-

DE/MS and (B) 2-DLC/MS-MS based on theoretical mass and pI. 2-DLC/MS-MS is

capable of identifying proportionally more basic and high mass proteins than 2-DE/MS.

Supplementary Figure 5. Comparison of identified C. jejuni JHH1 proteins from (A) 2-

DE/MS and (B) 2-DLC/MS-MS based on theoretical GRAVY value and number of

predicted TMR.

Supplementary Figure 6. Classification of sub-cellular location for proteins identified

from C. jejuni JHH1 membrane protein-enriched fractions using (A) 2-DE/MS and (B) 2-

DLC/MS-MS. Location was predicted using the program PSORTb v2.0.

Supplementary Figure 7. Immunoreactive proteins in C. jejuni JHH1 separated on (a)

pH 4-7, and (b) pH 6-11 2-DE gels. Spot numbers refer to Table 3.

Cj No.

Spot No. Identification MASC Pept. / % TpI / Tmass GRAVY LipoP SP TMR Location

Cj0029 74 Cytoplasmic L-Asparaginase [AnsA] Q9PJ82_CAMJE 181 7/26% 9.01 / 35393 0.101 N Y 1 Outer membrane Cj0059c 54 Putative Flagellar Motor Switch Protein FliY Q9PJ60_CAMJE 161 9/33% 4.58 / 31023 -0.119 N N 1 Flagellum Cj0069 52 Hypothetical Protein Q9PJ50_CAMJE 151 15/54% 5.23 / 38792 -0.447 N N 0 Unknown

Cj0073c 61 Conserved Hypothetical Protein Q9PJ48_CAMJE 358 13/61% 7.06 / 24596 -0.177 N N 0 Unknown Cj0113 1 Peptidoglycan-Associated Lipoprotein (Omp18) Q46123_CAMJE 140 4/23% 6.31 / 17828 -0.393 Y Y 1 Lipoprotein

Cj0129c 2 Outer Membrane Protein [Omp85 Family] Q9PIZ8_CAMJE 134 18/25% 5.50 / 83198 -0.365 N Y 2 Outer membrane Cj0144 3 Methyl-Accepting Chemotaxis Signal Transduction Protein Q9PIY3_CAMJE 149 5/10% 4.97 / 72362 -0.075 N Y 3 Inner membrane

Cj0262c 5 Putative Methyl-Accepting Chemotaxis Signal Transduction Protein Q9PIN3_CAMJE 153 12/19% 4.94 / 72831 -0.154 N Y 3 Inner membrane Cj0275 6 ATP-Dependant Clp Protease ATP-Binding Subunit ClpX CLPX_CAMJE 141 8/23% 5.16 / 47049 -0.227 N N 1 Cytoplasmic Cj0276 7 Cell Shape-Determining Protein [MreB] Q9PIL9_CAMJE 141 14/41% 5.22 / 37695 0.072 N N 2 Cytoplasmic

Cj0283c 8 Chemotaxis Protein CheW Q9PIL3_CAMJE 89 5/41% 5.48 / 19516 -0.245 N N 0 Cytoplasmic Cj0285c 9 Chemotaxis Protein CheV Q9PIL1_CAMJE 176 6/26% 4.92 / 35858 -0.181 N N 0 Cytoplasmic Cj0319 10 Flagellar Motor Switch Protein FliG Q9PII0_CAMJE 138 7/21% 4.95 / 38429 -0.130 N N 0 Flagellum

Cj0355c 11 Two-Component Response Regulator Q9PIE6_CAMJE 185 5/25% 5.28 / 25542 -0.401 N N 0 Cytoplasmic Cj0365c 12 Outer Membrane Channel Protein CmeC Q9PID7_CAMJE 175 14/30% 5.27 / 55418 -0.410 N Y 2 Outer membrane Cj0376 55 Putative Periplasmic Protein Q9PIC6_CAMJE 98 5/21% 5.04 / 32623 -0.447 N N 1 Periplasmic

Cj0406c 13 Putative Lipoprotein Q9PI99_CAMJE 166 8/34% 5.14 / 33152 -0.521 Y Y 1 Lipoprotein Cj0420 63 Putative Periplasmic Protein Q9PI85_CAMJE 334 10/38% 8.83 / 21038 -0.389 N Y 1 Periplasmic Cj0427 14 Hypothetical Protein Q9PI78_CAMJE 118 4/58% 5.31 / 13378 -0.973 N N 0 Cytoplasmic Cj0470 15 Elongation Factor Tu EFTU_CAMJE 318 10/31% 5.11 / 43594 -0.233 N N 0 Cytoplasmic Cj0475 68 50S Ribosomal Protein L1 [RplA] RL1_CAMJE 536 14/66% 9.61 / 25047 -0.260 N N 0 Ribosomal Cj0477 17 50S Ribosomal Protein L7 / L12 [RplL] RL7_CAMJE 186 3/35% 4.70 / 13073 0.039 N N 0 Ribosomal Cj0493 18 Elongation Factor G [EF-G] [FusA] EFG_CAMJE 136 8/14% 5.07 / 76719 -0.374 N N 0 Cytoplasmic Cj0511 70 Putative Secreted Carboxyl-Terminal Protease [CtpA] Q9PI00_CAMJE 199 17/35% 8.51 / 48876 -0.289 N Y 3 Secreted Cj0534 65 Succinyl-CoA Synthetase Alpha Chain [SucD] Q9PHY0_CAMJE 369 14/54% 7.65 / 30020 0.199 N N 2 Unknown Cj0536 78 OorA Subunit 2-Oxoglutarate:Acceptor Oxidoreductase QOPAY0_CAMJE 156 10/33% 6.12 / 41052 -0.109 N N 1 Cytoplasmic Cj0537 77 OorB Subunit 2-Oxoglutarate:Acceptor Oxidoreductase Q9PHX7_CAMJE 98 7/23% 7.99 / 31207 -0.247 N N 0 Cytoplasmic Cj0538 19 OorC Subunit 2-Oxoglutarate:Acceptor Oxidoreductase Q9PHX6_CAMJE 188 5/51% 5.68 / 20097 -0.092 N N 1 Unknown Cj0551 20 Elongation Factor P EFP_CAMJE 171 5/28% 5.18 / 21098 -0.359 N N 0 Cytoplasmic

Cj0561c 21 Putative Periplasmic Protein Q9PHV4_CAMJE 256 7/24% 5.53 / 34963 -0.399 N Y 1 Periplasmic Cj0596 69 Cell-Binding Factor 2 Major Antigen Peb4A CBF2_CAMJE 348 13/39% 9.23 / 30518 -0.462 N Y 1 Periplasmic Cj0599 22 Putative OmpA Family Membrane Protein Q9PHR7_CAMJE 47 3/21% 5.12 / 36435 -0.476 N N 1 Periplasmic

Cj0639c 23 Adenylate Kinase [Adk] KAD_CAMJE 58 4/21% 5.11 / 21407 -0.122 N N 0 Cytoplasmic

Cj0695 51 Cell Division Protein FtsA Q9PPL2_CAMJE 183 12/28% 5.12 / 50789 -0.141 N N 2 Cytoplasmic Cj0698 24 Flagellar Basal-Body Rod Protein FlgG Q9PPK9_CAMJE 87 5/29% 4.65 / 27731 -0.122 N N 0 Flagellum Cj0706 25 Conserved Hypothetical Protein Q9PPK1_CAMJE 99 5/16% 5.47 / 27811 -0.779 N N 0 Cytoplasmic

Cj0734c 26 Histidine-Binding Protein HisJ (CjaC) HISJ_CAMJE 315 8/40% 5.35 / 28531 -0.429 Y Y 1 Lipoprotein Cj0755 56 Ferric Enterobactin Uptake Receptor CfrA Q9PPG3_CAMJE 165 16/21% 5.29 / 77506 -0.445 N Y 1 Outer membrane Cj0778 67 Major Antigenic Peptide PEB2 Q9PPE1_CAMJE 252 10/31% 9.46 / 27320 -0.247 N Y 1 Periplasmic

Cj0893c 27 30S Ribosomal Protein S1 Q9PP38_CAMJE 238 13/28% 5.34 / 62795 -0.440 N N 0 Ribosomal Cj0921c 64 Major Cell-Binding Factor PEB1 [Peb1a] PEB1_CAMJE 472 14/62% 8.94 / 28177 -0.104 N Y 1 Periplasmic Cj0922c 76 ABC Transporter ATP-Binding Protein PEB1C [Peb1C] PEB1C_CAMJE 185 7/31% 8.74 / 27228 -0.199 N N 0 Inner membrane Cj0950c 28 Putative Lipoprotein Q9PNY3_CAMJE 160 5/36% 7.16 / 16162 -0.358 Y Y 1 Lipoprotein Cj0982c 29 Putative Amino Acid Transporter Periplasmic [Surface Antigen CjaA] Q9PNV7_CAMJE 248 8/32% 6.14 / 30910 -0.264 N Y 1 Periplasmic Cj1026c 30 Putative Lipoprotein Q9PNR4_CAMJE 168 6/30% 7.74 / 18524 -0.108 Y Y 1 Lipoprotein Cj1031 31 Putative Outer Membrane Component of Efflux System Q9PNR0_CAMJE 113 13/35% 6.04 / 48970 -0.420 N N 1 Outer membrane

Cj1058c 73 Inosine-5'-Monophosphate Dehydrogenase Q9PNN3_CAMJE 291 21/48% 7.15 / 52151 -0.060 N N 0 Cytoplasmic Cj1106 32 Putative Periplasmic Thioredoxin Q9PN18_CAMJE 71 2/15% 5.33 / 22965 -0.311 N Y 1 Periplasmic

Cj1131c 33 UDP-Glucose-4-Epimerase [GalE] Q9PNG3_CAMJE 64 4/14% 5.72 / 36733 -0.215 N N 0 Cytoplasmic Cj1170c 34 50kDa Outer Membrane Protein [Omp50] Q9PNC5_CAMJE 52 8/19% 6.14 / 53339 -0.499 N Y 2 Outer membrane Cj1182c 35 30S Ribosomal Protein S2 [RpsB] RS2_CAMJE 121 7/20% 5.69 / 30225 -0.493 N N 0 Ribosomal Cj1186c 60 Putative Ubiquinol-Cytochrome C Reductase Iron-Sulfur Subunit Q9PNA9_CAMJE 73 3/21% 7.63 / 18116 -0.114 N Y 2 Inner membrane Cj1227c 53 DNA-Binding Response Regulator OmpR Q9PN70_CAMJE 216 8/45% 5.63 / 25477 -0.329 N N 0 Cytoplasmic Cj1228c 72 Serine Protease DO Q9PN69_CAMJE 254 14/33% 8.86 / 51006 -0.282 N Y 1 Periplasmic Cj1259 36 Major Outer Membrane Protein (PorA) PORA_CAMJE 157 14/47% 4.71 / 45687 -0.179 N Y 2 Outer membrane Cj1261 37 Two-Component Response Regulator RacR O68795_CAMJE 65 4/16% 6.45 / 25398 -0.311 N N 0 Cytoplasmic

Cj1279c 38 Putative Fibronectin-Domain Containing Lipoprotein Q9PN19_CAMJE 200 10/34% 6.12 / 46124 -0.489 Y Y 1 Lipoprotein Cj1339c 57 Flagellin (FlaA) FLA1_CAMJE 217 15/37% 5.60 / 58907 -0.123 N N 3 Flagellum Cj1370 39 Putative Nucleotide Phosphoribosyltransferase Q9PMT0_CAMJE 358 8/55% 5.26 / 17034 -0.098 N N 0 Cytoplasmic Cj1380 66 Putative Periplasmic Protein Q9PMS0_CAMJE 442 14/58% 8.84 / 26596 -0.391 N Y 1 Periplasmic Cj1408 40 Putative Flagellar Protein [FliL] Q9PMP9_CAMJE 70 2/16% 4.93 / 19848 -0.137 N N 1 Flagellum

Cj1419c 41 Putative Methyltransferase Q9PMN8_CAMJE 200 7/30% 5.92 / 29704 -0.646 N N 0 Cytoplasmic Cj1420c 42 Putative Methyltransferase Q9PMN7_CAMJE 50 3/12% 6.26 / 29570 -0.529 N N 0 Cytoplasmic Cj1425c 79 Putative D-Glycero-D-Manno-Heptose 7-Phosphate Kinase HddA 176 12 / 32% 6.27 / 38100 -0.295 N N 2 Cytoplasmic Cj1462 43 Flagellar P-Ring Protein [FlgI] FLGI_CAMJE 76 8/28% 5.59 / 37040 -0.026 N Y 1 Periplasmic

Cj1478c 44 / 44F Outer Membrane Fibronectin-Binding Protein CadF Q9PMI4_CAMJE 192 10/34% 5.89 / 35997 -0.675 N Y 1 Outer membrane

Cj1506c 45 Putative MCP-Type Signal Transduction Protein Q9PMF7_CAMJE 136 10/19% 5.02 / 77268 -0.125 N Y 3 Inner membrane Cj1564 46 Methyl-Accepting Chemotaxis Protein 72 9/14% 5.47 / 73121 -0.105 N Y 3 Integral membrane Cj1595 47 DNA-Directed RNA Polymerase Alpha Chain [RpoA] RPOA_CAMJE 83 6/20% 4.97 / 37686 -0.195 N N 0 Cytoplasmic Cj1643 71 Putative Periplasmic Protein Q9PM33_CAMJE 257 13/43% 9.04 / 41375 -0.231 N N 1 Periplasmic

Cj1659 58 Periplasmic Protein P19 Q9PM18_CAMJE 139 5/51% 5.23 / 19642 -0.228 N Y 1 Periplasmic Cj1663 48 Putative ABC Transport System ATP-Binding Protein Q9PM14_CAMJE 53 3/24% 5.42 / 24459 -0.278 N N 0 Inner membrane

Cj1681c 49 CysQ Protein Homolog Q9PLZ6_CAMJE 64 4/17% 5.54 / 28977 -0.364 N N 0 Cytoplasmic Cj1695c 62 50S Ribosomal Protein L5 [RplE] RL5_CAMJE 535 17/78% 9.67 / 20213 -0.113 N N 0 Ribosomal Cj1721c 75 Possible Outer Memebrane Protein 402 11/61% 8.99 / 24029 -0.161 N Y 1 Outer membrane Cj1725 59 Putative Periplasmic Protein Q9PLV3_CAMJE 387 10/57% 5.73 / 21235 0.111 N Y 2 Periplasmic

Cj1729c 50 Flagellar Hook Subunit Protein FlgE Q9PLU9_CAMJE 75 3 4.86 / 91918 -0.422 N N 0 Flagellum

Supplementary Table 1. Identification of proteins from C. jejuni JHH1 membrane protein-enriched fractions separated by 2-DE and identified by MALDI TOF/TOF MS Shaded boxes represent proteins also identified in the 2-DLC/MS-MS data set and shown in Supplementary Table 2. Spot No. refers to Fig.2a,b. 44F signifies a lower mass variant of spot 44.

Cj No. Identification MASC Pept.

TpI / Tmass GRAVY LipoP SP TMR Location

Cj0003 DNA Gyrase Subunit B [GyrB] GYRB_CAMJE 240 7 5.39 / 86648 -0.359 N N 0 Cytoplasmic Cj0017c Putative ATP/GTP Binding Protein (Putative Disulfide Bond Formation Protein DsbI) [CjaE] Q9PJ94_CAMJE 1028 14 8.57 / 56769 0.049 N Y 5 Integral membrane Cj0019c MCP-Domain Signal Transduction Protein Q9PJ92_CAMJE 815 15 5.46 / 66845 -0.263 N Y 2 Inner membrane Cj0026c Thymidylate Synthase ThyX THYX_CAMJE 58 3 7.65 / 23809 -0.395 N N 0 Cytoplasmic Cj0027 CTP Synthase [PyrG] PYRG_CAMJE 199 10 6.32 / 60400 -0.174 N Y 2 Cytoplasmic

Cj0034c Putative Periplasmic Protein Q9PJ77_CAMJE 671 8 7.73 / 26456 -0.333 N Y 1 Periplasmic Cj0053c Probable tRNA (5-Methylaminomethyl-2-Thiouridylate)-Methyltransferase [TrmU] TRMU_CAMJE 58 2 8.26 / 38419 -0.322 N N 0 Cytoplasmic Cj0061c RNA Polymerase Sigma Factor For Flagellar Operon [FliA] Q9PJ58_CAMJE 47 1 5.04 / 28416 -0.658 N N 0 Flagellum Cj0063c Putative ATP-Binding Protein [ATPase ParA Family] Q9PJ56_CAMJE 92 5 6.86 / 31861 -0.125 N N 1 Outer membrane Cj0064c Flagellar Biosynthesis Protein FlhF FLHF_CAMJE 480 11 5.71 / 53356 -0.535 N N 0 Flagellum Cj0068 Protease PspA Q9PJ51_CAMJE 66 3 9.04 / 32583 0.050 N Y 1 Outer membrane Cj0069 Hypothetical Protein Q9PJ50_CAMJE 1174 11 5.23 / 38792 -0.447 N N 0 Unknown

Cj0073c Conserved Hypothetical Protein Q9PJ48_CAMJE 382 9 7.06 / 24596 -0.177 N N 0 Unknown Cj0074c Putative Iron-Sulfur Protein Q9PJ47_CAMJE 128 8 6.51 / 54220 -0.372 N N 1 Inner membrane Cj0076c L-Lactate Permease [LctP] Q9PJ45_CAMJE 138 4 8.48 / 60119 0.958 N N 15 Integral membrane Cj0088 Anaerobic C4-Dicarboxylate Transporter DcuA Q9PJ38_CAMJE 65 3 8.14 / 46709 1.033 N N 12 Integral membrane Cj0091 Putative Lipoprotein Q9PJ35_CAMJE 164 7 9.08 / 22446 -0.281 Y Y 1 Lipoprotein Cj0094 50S Ribosomal Protein L21 [RplU] Q9PJ32_CAMJE 160 3 10.04 / 11608 -0.427 N N 0 Ribosomal Cj0098 Methionyl-tRNA Formyltransferase [Fmt] FMT_CAMJE 157 4 8.65 / 34069 -0.124 N N 0 Cytoplasmic Cj0100 ParA Family Protein Q9PJ26_CAMJE 253 2 6.75 / 28731 0.012 N N 1 Unknown Cj0101 Chromosome Partitioning Protein ParB PARB_CAMJE 518 10 8.89 / 32028 -0.468 N N 0 Cytoplasmic Cj0102 ATP Synthase F0 B' Subunit [AtpF'] Q9PJ24_CAMJE 85 2 5.63 / 16352 -0.423 N N 1 Inner membrane Cj0103 ATP Synthase F0 B Subunit [AtpF] Q9PJ23_CAMJE 66 4 8.86 / 19732 -0.181 N Y 2 Inner membrane Cj0109 Putative MotA/ ExbB / TolQ Family Transport Protein [ExbB3] Q9PJ17_CAMJE 56 3 8.91 / 20445 0.513 N N 3 Integral membrane Cj0110 Putative ExbD / TolR Family Transport Protein Q9PJ16_CAMJE 59 1 5.21 / 14591 -0.126 N N 1 Integral membrane Cj0112 Putative TolB Precursor Protein TOLB_CAMJE 547 12 8.60 / 44778 -0.162 N Y 2 Periplasmic Cj0113 Peptidoglycan-Associated Lipoprotein (Omp18) Q46123_CAMJE 1285 7 6.31 / 17828 -0.393 Y Y 1 Lipoprotein Cj0114 Putative Periplasmic Protein Q9PJ13_CAMJE 85 2 8.92 / 35417 -0.804 N Y 1 Periplasmic Cj0115 FKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase [SlyD] Q9PJ12_CAMJE 92 2 4.75 / 20132 -0.303 N N 0 Cytoplasmic Cj0118 Conserved Hypothetical Protein Q9PJ09_CAMJE 67 2 9.04 / 28388 -0.296 N N 0 Unknown

Cj0124c Putative Membrane Protein Q9PJ03_CAMJE 157 1 8.59 / 38628 -0.122 N Y 2 Integral membrane Cj0127c Acetyl-CoA Carboxylase Carboxyl Transferase Beta Subunit [AccD] Q9PJ00_CAMJE 166 4 7.57 / 30951 -0.125 N N 2 Unknown Cj0129c Outer Membrane Protein [Omp85 Family] Q9PIZ8_CAMJE 532 12 5.50 / 83198 -0.365 N Y 2 Outer membrane Cj0131 Putative Peptidase M23 Family Protein Q9PIZ6_CAMJE 393 11 8.63 / 51724 -0.234 N Y 2 Periplasmic

Cj0134 Putative Homoserine Kinase (ThrB) KHSE_CAMJE 63 2 6.84 / 32501 0.013 N N 3 Unknown Cj0136 Translation Initiation Factor IF-2 [InfB] IF2_CAMJE 198 12 5.57 / 96125 -0.461 N N 1 Cytoplasmic Cj0139 Putative Endonuclease Q9PIY8_CAMJE 87 4 5.67 / 92496 -0.605 N N 0 Unknown Cj0144 Methyl-Accepting Chemotaxis Signal Transduction Protein Q9PIY3_CAMJE 4222 25 4.97 / 72362 -0.075 N Y 3 Inner membrane

Cj0147c Thioredoxin [Trx] Q9PIY0_CAMJE 113 3 4.54 / 11352 -0.025 N N 0 Cytoplasmic Cj0149c Homoserine Dehydrogenase [Hom] Q9PIX8_CAMJE 53 2 6.99 / 45918 0.032 N N 1 Unknown Cj0152c Putative Membrane Protein Q9PIX5_CAMJE 125 5 5.21 / 35557 -0.457 N N 1 Integral membrane Cj0161c Putative Molybdenum Cofactor Biosynthesis Protein A MOAA_CAMJE 67 4 7.54 / 36637 -0.251 N N 0 Cytoplasmic Cj0172c Putative Saccharopine Dehydrogenase Q9PIV7_CAMJE 45 3 5.32 / 44943 -0.229 N N 3 Unknown Cj0183 Putative Integral Membrane Protein Haemolysin Domain Q9PIU6_CAMJE 201 4 5.03 / 51010 0.113 N N 4 Integral membrane

Cj0186c Putative TerC-Family Integral Membrane Protein Q9PIU3_CAMJE 46 1 5.57 / 26442 0.998 N N 6 Integral membrane Cj0192c ATP-Dependent Clp Protease Proteolytic Subunit ClpP CLPP_CAMJE 65 2 6.74 / 21650 -0.061 N N 2 Cytoplasmic Cj0208 Putative DNA Modification Methylase Q9PIS1_CAMJE 48 3 9.26 / 42324 -0.465 N N 0 Cytoplasmic

Cj0230c Hypothetical Protein Putative Phosphoribosyltransferase Q9PIR4_CAMJE 184 4 5.78 / 52263 -0.202 N N 1 Unknown Cj0233c Putative Orotate Phosphoribosyltransferase [PyrE] PYRE_CAMJE 71 2 8.35 / 22212 -0.117 N N 2 Cytoplasmic Cj0238 Putative Mechanosensitive Ion Channel Family Protein Q9PIQ6_CAMJE 58 2 6.40 / 71371 0.180 N N 7 Integral membrane

Cj0239c NifU Protein Homolog Q9PIQ5_CAMJE 273 2 4.76 / 35490 -0.193 N N 1 Cytoplasmic Cj0240c Cysteine Desulfurase [NifS Homolog] Q9PIQ4_CAMJE 159 7 5.79 / 43053 -0.119 N N 0 Unknown Cj0245 50S Ribosomal Protein L20 [RplT] RL20_CAMJE 136 4 11.55 / 13801 -0.643 N N 0 Ribosomal Cj0248 Hypothetical Protein Q9PIP7_CAMJE 408 11 5.84 / 31960 -0.016 N N 2 Integral membrane

Cj0255c Exodeoxyribonuclease Q9PIP0_CAMJE 239 4 6.11 / 29750 -0.425 N N 0 Cytoplasmic Cj0256 Putative Sulfatase Family Integral Membrane Protein Q9PIN9_CAMJE 81 2 8.34 / 59335 0.077 N N 6 Integral membrane Cj0262c Putative Methyl-Accepting Chemotaxis Signal Transduction Protein Q9PIN3_CAMJE 3941 22 4.94 / 72831 -0.154 N Y 3 Inner membrane Cj0266c Putative Integral Membrane Protein Q9PIM9_CAMJE 83 2 9.05 / 19512 0.375 N Y 2 Integral membrane Cj0268c Putative Transmembrane Protein Q9PIM7_CAMJE 889 16 8.93 / 40259 -0.373 N N 1 Integral membrane Cj0271 Bacterioferritin Comigratory Protein Homolog Q9PIM4_CAMJE 334 3 7.70 / 16711 -0.210 N N 0 Cytoplasmic Cj0274 Acyl-[Acyl-Carrier-Protein]-UDP-N-Acetylglucosamine O-Acyltransferase LPXA_CAMJE 421 6 7.67 / 28650 -0.142 N N 0 Cytoplasmic Cj0275 ATP-Dependant Clp Protease ATP-Binding Subunit ClpX CLPX_CAMJE 518 14 5.16 / 47049 -0.227 N N 1 Cytoplasmic Cj0276 Cell Shape-Determining Protein [MreB] Q9PIL9_CAMJE 777 16 5.22 / 37695 0.072 N N 2 Cytoplasmic Cj0277 Rod-Shape Determining Protein MreC Homolog Q9PIL8_CAMJE 97 3 9.03 / 28381 -0.059 N N 1 Periplasmic Cj0279 Carbamoyl-Phosphate Synthase Large Chain CARB_CAMJE 53 8 5.59 / 120940 -0.125 N N 2 Unknown

Cj0281c Putative Transaldolase [Tal] TAL_CAMJE 50 1 6.38 / 36917 -0.267 N N 0 Cytoplasmic Cj0282c Putative Phosphoserine Phosphatase SerB Q9PIL4_CAMJE 67 1 6.59 / 23009 0.085 N N 0 Cytoplasmic Cj0283c Chemotaxis Protein CheW Q9PIL3_CAMJE 78 3 5.48 / 19516 -0.245 N N 0 Cytoplasmic Cj0284c Chemotaxis Histidine Kinase CheA Q9PIL2_CAMJE 134 8 4.89 / 85299 -0.272 N N 0 Cytoplasmic Cj0285c Chemotaxis Protein CheV Q9PIL1_CAMJE 192 5 4.92 / 35858 -0.181 N N 0 Cytoplasmic Cj0289c Major Antigenic Peptide PEB3 Q9PIK7_CAMJE 646 7 9.42 / 27536 -0.276 N Y 1 Periplasmic Cj0293 Multifunctional Protein SurE Homolog SURE_CAMJE 62 1 9.35 / 29012 -0.301 N N 0 Cytoplasmic

Cj0307 Adenosylmethionine-8-Amino-7-Oxononanoate Aminotransferase [BioA] Q9PIJ2_CAMJE 59 4 6.70 / 48668 -0.009 N N 0 Unknown Cj0311 Putative 50S Ribosomal Protein L25 [RplY] RL25_CAMJE 138 5 9.73 / 19461 0.108 N N 0 Ribosomal Cj0318 Flagellar M-Ring Protein [FliF] Q9PII1_CAMJE 254 8 5.05 / 62602 -0.358 N Y 2 Flagellum Cj0319 Flagellar Motor Switch Protein FliG Q9PII0_CAMJE 113 2 4.95 / 38429 -0.130 N N 0 Flagellum Cj0324 Ubiquinone / Menaquinone Biosynthesis Methyltransferase [UbiE] UBIE_CAMJE 121 3 9.24 / 26516 -0.091 N N 0 Unknown

Cj0329c Putative Fatty Acid / Phospholipid Synthesis Protein PlsX PLSX_CAMJE 77 3 8.76 / 35884 -0.084 N N 1 Unknown Cj0334 Alkyl Hydroperoxide Reductase Subunit C [AhpC] Q9ZI13_CAMJE 473 4 5.66 / 21947 -0.148 N N 0 Cytoplasmic

Cj0336c Putative Flagellar Motor Protein MotB [MotB] Q9PIG4_CAMJE 282 3 7.62 / 27813 -0.500 N N 1 Flagellum Cj0337c Putative Flagellar Motor Protein MotA [MotA] Q9PIG3_CAMJE 107 4 5.21 / 28125 0.258 N N 4 Flagellum Cj0339 Putative Major Facilitator Superfamily Transport Protein Q9PIG1_CAMJE 47 1 9.37 / 49918 0.526 N N 11 Integral membrane Cj0342c Excinuclease ABC Subunit A [UvrA] Q9PIF8_CAMJE 53 5 8.76 / 105618 -0.293 N N 1 Cytoplasmic Cj0348 Tryptophan Synthase Beta Chain [TrpB] TRPB_CAMJE 310 4 9.22 / 42947 -0.079 N N 0 Cytoplasmic Cj0349 Tryptophan Synthase Alpha Chain [TrpA] TRPA_CAMJE 114 4 8.25 / 27966 0.129 N N 1 Cytoplasmic Cj0350 Hypothetical Protein Q9PIF0_CAMJE 95 4 4.75 / 16278 -0.209 N N 0 Cytoplasmic Cj0355c Two-Component Response Regulator Q9PIE6_CAMJE 107 4 5.28 / 25542 -0.401 N N 0 Cytoplasmic Cj0365c Outer Membrane Channel Protein CmeC Q9PID7_CAMJE 256 6 5.27 / 55418 -0.410 N Y 2 Outer membrane Cj0366c Transmembrane Efflux Protein CmeB Q9PID6_CAMJE 656 9 6.48 / 113967 0.259 N Y 12 Integral membrane Cj0367c Membrane Fusion Component of Efflux System [CmeA] Q9PID5_CAMJE 301 5 8.29 / 40118 -0.218 N Y 1 Inner membrane Cj0371 Hypothetical UPF0323 Lipoprotein Precursor Y371_CAMJE 201 3 8.95 / 20697 -0.335 Y Y 2 Lipoprotein Cj0386 Putative GTP-Binding Protein ENGA_CAMJE 132 8 8.28 / 52640 -0.348 N N 0 Unknown Cj0387 Shikimate Kinase [AroK] Q9PIB5_CAMJE 60 3 8.82 / 19243 -0.403 N N 0 Cytoplasmic Cj0389 Seryl-tRNA Synthetase [SerS] SYS_CAMJE 78 3 5.73 / 46583 -0.428 N N 0 Cytoplasmic Cj0390 Putative Transmembrane Protein Q9PIB2_CAMJE 141 4 5.13 / 93529 -0.361 N N 2 Integral membrane

Cj0392c Pyruvate Kinase Q9PIB0_CAMJE 80 2 5.89 / 53857 -0.265 N N 0 Cytoplasmic Cj0393c Probable Malate:Quinone Oxidoreductase MQO_CAMJE 1253 15 8.23 / 50586 -0.235 N N 1 Inner membrane Cj0396c Putative Lipoprotein Q9PIA7_CAMJE 205 5 8.18 / 37384 0.037 Y Y 1 Lipoprotein Cj0397c Hypothetical Protein Q9PIA6_CAMJE 97 3 9.02 / 22312 -0.402 N N 1 Integral membrane Cj0399 Colicin V Production Protein Homolog Q9PIA4_CAMJE 65 2 5.83 / 20659 0.650 N N 4 Integral membrane Cj0402 Serine Hydroxymethyltransferase [GlyA] GLYA_CAMJE 91 5 5.93 / 45777 -0.187 N N 0 Cytoplasmic Cj0403 Hypothetical Protein Q9PIA2_CAMJE 262 6 9.50 / 21348 -0.349 N N 0 Unknown Cj0404 Putative Transmembrane Protein Q9PIA1_CAMJE 649 6 5.74 / 30773 -0.727 N N 1 Integral membrane Cj0406c Putative Lipoprotein Q9PI99_CAMJE 325 7 5.14 / 33152 -0.521 Y Y 1 Lipoprotein Cj0408 Fumarate Reductase Cytochrome B Subunit FrdC Q9PI97_CAMJE 62 4 9.37 / 30028 0.372 N N 5 Integral membrane Cj0409 Fumarate Reductase Flavoprotein Subunit Q9PI96_CAMJE 706 16 6.36 / 73788 -0.398 N N 1 Cytoplasmic Cj0410 Fumarate Reductase Iron-Sulfur Protein FrdB Q9PI95_CAMJE 121 3 5.37 / 27558 -0.204 N N 1 Cytoplasmic Cj0412 Putative ATP/GTP Binding Protein Q9PI93_CAMJE 89 4 5.25 / 71303 -0.313 N N 0 Cytoplasmic Cj0420 Putative Periplasmic Protein Q9PI85_CAMJE 148 5 8.83 / 21038 -0.389 N Y 1 Periplasmic Cj0426 Putative ABC Transporter ATP-Binding Protein Q9PI79_CAMJE 154 8 5.20 / 59612 -0.286 N N 1 Inner membrane

Cj0429c Conserved Hypothetical Protein Q9PI76_CAMJE 143 2 7.68 / 22342 -0.208 N N 0 Cytoplasmic Cj0433c Phospho-N-Acetylmuramoyl-Pentapeptide Transferase MraY MRAY_CAMJE 64 1 8.53 / 39240 0.890 N N 10 Integral membrane Cj0436 Putative Pyridoxamine 5'-Phosphate Oxidase Q9PI69_CAMJE 167 2 8.87 / 15750 -0.196 N N 0 Unknown Cj0441 Acyl Carrier Protein ACP [AcpP] ACP_CAMJE 154 1 4.04 / 8597 0.079 N N 0 Cytoplasmic Cj0443 Acetyl-CoA Carboxylase Carboxyl Transferase Alpha Subunit [AccA] Q9PI62_CAMJE 181 5 5.44 / 34253 -0.267 N N 2 Cytoplasmic Cj0450 50S Ribosomal Protein L28 RL28_CAMJE 60 3 12.01 / 7195 -0.736 N N 0 Ribosomal Cj0453 Thiamine Biosynthesis Protein ThiC THIC_CAMJE 46 3 5.64 / 47524 -0.180 N N 1 Cytoplasmic

Cj0455c Putative Membrane Protein Q9PI53_CAMJE 45 2 5.41 / 13461 0.102 N N 1 Integral membrane Cj0458c Putative tRNA 2-Methylthioadenosine Synthase Q9PI50_CAMJE 87 7 7.95 / 49473 -0.374 N N 0 Cytoplasmic Cj0460 Transcription Termination Factor NusA Q9PI48_CAMJE 152 5 5.51 / 40671 -0.304 N N 0 Cytoplasmic Cj0462 Putative Radical SAM Domain-Containing Protein Q9PI46_CAMJE 237 6 6.37 / 39758 -0.268 N N 0 Cytoplasmic Cj0469 Amino Acid ABC Transporter ATP-Binding Protein Q9PI39_CAMJE 52 2 7.06 / 29043 -0.247 N N 0 Inner membrane Cj0470 Elongation Factor Tu EFTU_CAMJE 946 16 5.11 / 43594 -0.233 N N 0 Cytoplasmic Cj0473 Transcription Antitermination Protein NusG NUSG_CAMJE 69 4 6.00 / 20195 -0.295 N N 0 Cytoplasmic Cj0474 50S Ribosomal Protein L11 [RplK] RL11_CAMJE 120 2 9.68 / 15079 -0.148 N N 0 Ribosomal Cj0475 50S Ribosomal Protein L1 [RplA] RL1_CAMJE 329 4 9.61 / 25047 -0.260 N N 0 Ribosomal Cj0476 50S Ribosomal Protein L10 [RplJ] RL10_CAMJE 229 3 8.91 / 17715 -0.335 N N 0 Ribosomal Cj0477 50S Ribosomal Protein L7 / L12 [RplL] RL7_CAMJE 280 5 4.70 / 13073 0.039 N N 0 Ribosomal Cj0479 DNA-Directed RNA Polymerase Beta Subunit RPOC_CAMJE 1908 30 8.57 / 168822 -0.285 N N 0 Cytoplasmic Cj0491 30S Ribosomal Protein S12 [RpsL] RS12_CAMJE 156 4 10.96 / 14045 -0.573 N N 0 Ribosomal Cj0492 30S Ribosomal Protein S7 [RpsG] RS7_CAMJE 227 6 10.03 / 17691 -0.510 N N 0 Ribosomal Cj0493 Elongation Factor G [EF-G] [FusA] EFG_CAMJE 177 6 5.07 / 76719 -0.374 N N 0 Cytoplasmic Cj0506 Alanyl-tRNA Synthetase SYA_CAMJE 1021 29 6.04 / 94906 -0.394 N N 0 Cytoplasmic Cj0508 Penicillin-Binding Protein 1A [PbpA] Q9PI03_CAMJE 162 8 8.36 / 72628 -0.273 N Y 2 Outer membrane Cj0511 Putative Secreted Carboxyl-Terminal Protease [CtpA] Q9PI00_CAMJE 263 9 8.51 / 48876 -0.289 N Y 3 Secreted Cj0512 Phosphoribosylaminoimidazole-Succinocarboxamide Synthase (PurC) PUR7_CAMJE 1040 10 5.80 / 26938 -0.320 N N 0 Cytoplasmic Cj0514 Phosphoribosylformylglycinamidine Synthetase I PurQ PURQ_CAMJE 43 3 6.22 / 24931 -0.122 N N 0 Cytoplasmic Cj0515 Putative Periplasmic Protein Q9PHZ6_CAMJE 94 4 5.24 / 45603 -0.224 N Y 3 Periplasmic Cj0518 Chaperone Protein HtpG HTPG_CAMJE 58 2 5.09 / 69654 -0.500 N N 1 Cytoplasmic Cj0530 Putative Periplasmic Protein Q9PHY4_CAMJE 57 7 6.83 / 97720 -0.268 N Y 1 Periplasmic Cj0533 Succinyl-CoA Synthetase Beta Chain [SucC] SUCC_CAMJE 563 12 5.61 / 41752 -0.019 N N 1 Cytoplasmic Cj0534 Succinyl-CoA Synthetase Alpha Chain [SucD] Q9PHY0_CAMJE 667 9 7.65 / 30020 0.199 N N 2 Unknown Cj0535 2-Oxoglutarate:Acceptor Oxidoreducatse OorD Q9PHX9_CAMJE 68 1 6.87 / 11443 -0.053 N N 0 Cytoplasmic Cj0536 OorA Subunit 2-Oxoglutarate:Acceptor Oxidoreductase 1661 14 6.12 / 41052 -0.109 N N 2 Unknown Cj0537 OorB Subunit 2-Oxoglutarate:Acceptor Oxidoreductase Q9PHX7_CAMJE 539 7 7.99 / 31207 -0.247 N N 0 Cytoplasmic Cj0538 OorC Subunit 2-Oxoglutarate:Acceptor Oxidoreductase Q9PHX6_CAMJE 441 5 5.68 / 20097 -0.092 N N 1 Unknown Cj0542 Glutamyl-tRNA Reductase [HemA] HEM1_CAMJE 45 3 6.00 / 48697 -0.070 N N 0 Cytoplasmic Cj0546 Putative 3-Octaprenyl-4-Hydroxybenzoate Carboxy-Lyase Q9PHW8_CAMJE 174 9 5.92 / 69159 -0.241 N N 1 Cytoplasmic

Cj0554 Hypothetical Protein Q9PHW0_CAMJE 54 3 5.97 / 38850 -0.193 N N 2 Unknown Cj0561c Putative Periplasmic Protein Q9PHV4_CAMJE 48 1 5.53 / 34963 -0.399 N Y 1 Periplasmic Cj0562 Replicative DNA Helicase [DnaB] Q9PHV3_CAMJE 253 4 5.51 / 51867 -0.336 N N 0 Cytoplasmic Cj0572 GTP Cyclohydrolase II GCH2_CAMJE 48 2 5.61 / 37924 -0.198 N N 0 Cytoplasmic Cj0574 Acetolactate Synthase Large Subunit [IlvI] Q9PHU2_CAMJE 86 3 5.83 / 62928 -0.101 N N 2 Unknown Cj0576 UDP-3-O-[3-Hydroxymyristoyl] Glucosamine N-Acyltransferase [LpxD] LPXD_CAMJE 226 8 6.51 / 34678 -0.061 N N 0 Cytoplasmic

Cj0579c Sec-Independent Protein Translocase Protein TatB Homolog [TatB] TATB_CAMJE 175 4 5.68 / 15746 -0.620 N N 1 Inner membrane Cj0581 Putative NUDIX Hydrolase Family Protein NUDH_CAMJE 44 2 6.32 / 18584 -0.497 N N 0 Cytoplasmic Cj0582 Aspartokinase [LysC] Q9PHT4_CAMJE 46 2 5.47 / 42675 -0.007 N N 1 Unknown

Cj0591c Putative Lipoprotein Q9PHS5_CAMJE 49 1 5.60 / 8499 -0.488 Y Y 1 Lipoprotein Cj0592c Putative Periplasmic Protein Q9PHS4_CAMJE 77 2 5.18 / 16095 -0.838 N N 1 Periplasmic Cj0595c Endonuclease III Q9PHS1_CAMJE 81 5 8.98 / 23755 -0.236 N N 0 Cytoplasmic Cj0596 Cell-Binding Factor 2 Major Antigen Peb4A CBF2_CAMJE 66 4 9.23 / 30518 -0.462 N Y 1 Periplasmic Cj0598 Putative Membrane Protein Q9PHR8_CAMJE 220 9 5.06 / 55669 -0.444 N Y 4 Integral membrane Cj0599 Putative OmpA Family Membrane Protein Q9PHR7_CAMJE 57 4 5.12 / 36435 -0.476 N N 1 Periplasmic Cj0604 Putative Polyphosphate Kinase Q9PHR2_CAMJE 86 3 9.52 / 34096 -0.671 N N 0 Cytoplasmic Cj0605 Putative Amidohydrolase [Carboxypeptidase] Q9PHR1_CAMJE 70 1 5.80 / 43719 -0.108 N N 1 Unknown Cj0606 Putative Secretiob Protein HlyD Q9PHR0_CAMJE 62 3 5.41 / 42820 -0.241 N Y 1 Periplasmic Cj0607 ABC-Type Transmembrane Transport Macrolide-Specific Efflux Protein [MacB] Q9PHQ9_CAMJE 202 8 9.14 / 70272 0.114 N N 4 Integral membrane Cj0610c Putative Periplasmic Protein Q9PHQ6_CAMJE 85 4 6.68 / 38791 -0.420 N Y 1 Periplasmic Cj0623 Hydrogenase Isoenzymes Formation Protein [HypB] Q9PHP4_CAMJE 44 2 6.08 / 27544 -0.293 N N 0 Cytoplasmic Cj0625 Hydrogenase Isoenzymes Formation Protein [HypD] Q9PHP2_CAMJE 79 1 8.59 / 40205 0.017 N N 1 Cytoplasmic Cj0633 Putative Periplasmic Protein Q9PHN4_CAMJE 176 3 8.24 / 41445 -0.471 N Y 1 Periplasmic Cj0639c Adenylate Kinase [Adk] KAD_CAMJE 85 2 5.11 / 21407 -0.122 N N 0 Cytoplasmic Cj0642 Putative DNA Repair Protein RecN RECN_CAMJE 55 4 5.27 / 58522 -0.424 N N 0 Cytoplasmic Cj0643 Two-Component Response Regulator CbrR GGDEF-Domain Protein Q9PHM4_CAMJE 469 7 4.85 / 46666 -0.150 N N 1 Cytoplasmic Cj0646 RlpA Rare Lipoprotein Putative [RlpA] RLPA_CAMJE 480 4 9.52 / 29357 -0.241 Y Y 1 Lipoprotein

Cj0661c GTP-Binding Protein Era Homolog [Era] ERA_CAMJE 102 2 8.92 / 33171 -0.304 N N 0 Unknown Cj0662c ATP-Dependent Hsl Protease ATP-Binding Subunit HslU HSLU_CAMJE 139 4 5.71 / 49779 -0.496 N N 0 Cytoplasmic Cj0665c Arginosuccinate Synthase [ArgG] ASSY_CAMJE 125 1 5.76 / 45578 -0.338 N N 0 Cytoplasmic Cj0669 ABC-Transporter ATP-Binding Protein Q9PPN2_CAMJE 173 3 8.24 / 26651 0.028 N N 1 Inner membrane Cj0686 4-Hydroxy-3-Methylbut-2-En-1-Yl Diphosphate Synthase [IspG] ISPG_CAMJE 113 9 7.58 / 39365 -0.058 N N 0 Cytoplasmic Cj0688 Putative Phosphate Acetyltransferase [Pta] Q9PPL9_CAMJE 310 9 5.75 / 56177 -0.137 N N 0 Cytoplasmic Cj0689 Acetate Kinase ACKA_CAMJE 2053 18 6.18 / 44057 -0.152 N N 0 Cytoplasmic Cj0694 Putative Periplasmic Protein Q9PPL3_CAMJE 49 4 5.66 / 57507 -0.635 N N 1 Periplasmic Cj0695 Cell Division Protein FtsA Q9PPL2_CAMJE 391 8 5.12 / 50789 -0.141 N N 2 Cytoplasmic Cj0696 Cell Division Protein FtsZ Q9PPL1_CAMJE 180 6 5.05 / 39277 -0.042 N N 2 Cytoplasmic Cj0700 Hypothetical Protein Q9PPK7_CAMJE 43 1 4.44 / 26023 -0.626 N N 0 Cytoplasmic

Cj0709 Signal Recognition Particle Protein [Ffh] Q9PPJ8_CAMJE 147 8 9.18 / 48896 -0.144 N N 1 Inner membrane Cj0710 30S Ribosomal Protein S16 [RpsP] RS16_CAMJE 97 2 10.22 / 8678 -0.449 N N 0 Ribosomal Cj0712 Putative 16S rRNA Processing Protein RimM RIMM_CAMJE 51 3 6.20 / 21209 -0.401 N N 0 Cytoplasmic Cj0713 tRNA (Guanine-N(1)-)-Methyltransferase TRMD_CAMJE 44 1 8.92 / 27110 -0.365 N N 0 Cytoplasmic Cj0714 Ribosomal Protein L19 RL19_CAMJE 605 9 10.66 / 13681 -0.600 N N 0 Ribosomal Cj0726c Magnesium And Cobalt Transport Protein CorA Q9PPI1_CAMJE 42 3 5.02 / 38161 -0.093 N N 2 Integral membrane Cj0734c Histidine-Binding Protein HisJ (CjaC) HISJ_CAMJE 1003 11 5.35 / 28531 -0.429 Y Y 1 Lipoprotein Cj0762c Aspartate Aminotransferase [AspB] Q9PPF7_CAMJE 387 4 8.26 / 43184 -0.185 N N 0 Cytoplasmic Cj0763c Serine Acetyltransferase [CysE] Q9PPF6_CAMJE 86 2 8.98 / 23483 -0.053 N N 2 Cytoplasmic Cj0774c ABC Transport ATP-Binding Protein Q9PPE5_CAMJE 80 2 8.96 / 37997 -0.232 N N 0 Inner membrane Cj0776c Putative Periplasmic Protein Q9PPE3_CAMJE 142 7 9.14 / 39510 -0.757 N N 1 Periplasmic Cj0779 Thiol Peroxidase [Tpx] TPX_CAMJE 293 5 5.13 / 18440 0.217 N N 0 Cytoplasmic Cj0780 Periplasmic Nitrate Reductase Large Subunit [NapA] Q9PPD9_CAMJE 161 6 8.76 / 104943 -0.515 N Y 2 Periplasmic Cj0783 Periplasmic Nitrate Reductase Small Subunit [NapB] Q9PPD6_CAMJE 412 4 6.51 / 19113 -0.360 N Y 1 Periplasmic Cj0793 Sensor Transduction Histidine Kinase FlgS Q9PPC6_CAMJE 68 4 5.18 / 38443 -0.153 N N 1 Inner membrane

Cj0796c Putative Hydrolase Q9PPC4_CAMJE 47 1 9.33 / 27485 -0.067 N N 0 Unknown Cj0802 Cysteinyl-tRNA Synthetase [CysS] SYC_CAMJE 118 7 5.83 / 53513 -0.474 N N 0 Cytoplasmic Cj0803 Lipid Export ABC Transport Protein MsbA Q9PPB7_CAMJE 65 4 8.98 / 65327 0.096 N Y 6 Integral membrane Cj0804 Dihydroorotate Dehydrogenase [PyrD] Q9PPB6_CAMJE 367 8 8.29 / 39024 -0.037 N N 2 Unknown Cj0806 Dihydrodipicolinate Synthase DAPA_CAMJE 565 5 6.02 / 32670 -0.108 N N 0 Cytoplasmic Cj0810 NH3-Dependent NAD+ Synthetase NADE_CAMJE 67 2 5.42 / 27462 -0.089 N N 2 Unknown Cj0812 Threonine Synthase [ThrC] Q9PPA8_CAMJE 393 8 5.97 / 53841 -0.344 N N 0 Cytoplasmic Cj0831c tRNA (Uracil-5-)-Methyltransferase TRMA_CAMJE 65 3 8.47 / 42276 -0.297 N N 1 Cytoplasmic Cj0833c Putative Oxidoreductase Q9PP90_CAMJE 182 3 6.04 / 27975 -0.126 N N 0 Cytoplasmic Cj0835c Aconitate Hydratase 2 [AcnB] Q9PP88_CAMJE 346 10 6.04 / 92793 -0.184 N N 0 Cytoplasmic Cj0856 Signal Peptidase I [LepP] Q9PP67_CAMJE 70 3 6.15 / 32951 -0.367 N Y 1 Inner membrane

Cj0858c UDP-N-Acetylglucosamine 1-Carboxyvinyltransferase [MurA] MURA_CAMJE 282 10 5.93 / 45186 0.090 N N 0 Cytoplasmic Cj0882c Flagellar Biosynthesis Protein FlhA Q9PP48_CAMJE 180 4 5.12 / 78836 0.442 N Y 9 Flagellum Cj0886c Putative Cell Division Protein FtsK FTSK_CAMJE 72 6 5.35 / 107863 -0.310 N N 3 Integral membrane Cj0890c Putative Sensory Transduction Trancriptional Regulator Q9PP41_CAMJE 45 2 4.93 / 25642 -0.175 N N 0 Cytoplasmic Cj0893c 30S Ribosomal Protein S1 Q9PP38_CAMJE 1861 20 5.34 / 62795 -0.440 N N 0 Ribosomal Cj0894c 4-Hydroxy-3-Methylbut-2-Enyl Diphosphate Reductase [IspH] ISPH_CAMJE 145 4 8.70 / 31550 -0.338 N N 0 Cytoplasmic Cj0895c 3-Phosphoshikimate 1-Carboxyvinyltransferase [AroA] AROA_CAMJE 145 4 8.57 / 47288 0.023 N N 3 Cytoplasmic Cj0898 Putative HIT-Family Protein Q9PP33_CAMJE 291 3 6.19 / 13881 -0.166 N N 0 Cytoplasmic

Cj0899c 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate Synthesis Protein [ThiJ] Q9PP32_CAMJE 254 4 5.02 / 20154 0.325 N N 1 Cytoplasmic Cj0903c Putative Amino Acid-Transport Protein Q9PP28_CAMJE 874 7 9.13 / 53008 0.631 N N 10 Integral membrane Cj0910 Putative Periplasmic Protein Q9PP22_CAMJE 64 1 9.18 / 17445 0.120 N Y 1 Periplasmic

Cj0913c DNA-Binding Protein HU [Hup] DBH_CAMJE 406 2 10.29 / 10273 -0.173 N N 0 Cytoplasmic

Cj0917c Carbon Starvation Protein A Homolog Q9PP16_CAMJE 770 5 8.38 / 75792 0.805 N Y 17 Integral membrane Cj0920c Putative ABC-Type Amino Acid Transporter Protein Q9PP13_CAMJE 72 2 8.51 / 28013 0.560 N N 3 Integral membrane Cj0921c Major Cell-Binding Factor PEB1 [Peb1a] PEB1_CAMJE 242 6 8.94 / 28177 -0.104 N Y 1 Periplasmic Cj0922c ABC Transporter ATP-Binding Protein PEB1C [Peb1C] PEB1C_CAMJE 476 2 8.74 / 27228 -0.199 N N 0 Inner membrane Cj0923c Putative Chemotaxis Protein Methyltransferase CheR CHER_CAMJE 61 2 8.92 / 30654 -0.330 N N 0 Cytoplasmic Cj0925 Ribose 5-Phosphate Isomerase [RpiB] Q9PP08_CAMJE 115 3 8.22 / 16293 -0.027 N N 0 Cytoplasmic Cj0926 Putative Membrane Protein Q9PP07_CAMJE 114 3 9.27 / 13075 -0.145 N N 1 Integral membrane Cj0930 Putative GTP-Binding Protein YchF Q9PP03_CAMJE 263 5 5.28 / 40655 -0.233 N N 1 Cytoplasmic

Cj0932c Phosphoenolpyruvate Carboxykinase [PckA] PPCK_CAMJE 79 1 5.76 / 59054 -0.360 N N 0 Cytoplasmic Cj0942c Preprotein Translocase SecA Subunit [SecA] Q9PNZ1_CAMJE 395 12 5.59 / 97976 -0.514 N N 1 Inner membrane Cj0950c Putative Lipoprotein Q9PNY3_CAMJE 872 8 7.16 / 16162 -0.358 Y Y 1 Lipoprotein Cj0953c Bifunctional Purine Biosynthesis Protein PurH PUR9_CAMJE 52 2 5.49 / 56402 -0.171 N N 0 Cytoplasmic Cj0955 Phosphoribosylformylglycinamidine Synthase II (EC 6.3.5.3) [PurL] PURL_CAMJE 491 7 5.14 / 79309 -0.127 N N 2 Cytoplasmic

Cj0956c Putative tRNA Modification GTPase TrmE TRME_CAMJE 47 2 5.08 / 49144 -0.026 N N 1 Cytoplasmic Cj0957c Hypothetical Protein Q9PNX8_CAMJE 257 5 9.01 / 31893 -0.638 N N 0 Cytoplasmic Cj0958c Inner Membrane Protein OxaA OXAA_CAMJE 257 5 8.83 / 60827 -0.080 N N 5 Integral membrane Cj0982c Putative Amino Acid Transporter Solute-Binding Protein [Surface Antigen CjaA] Q9PNV7_CAMJE 597 11 6.14 / 30910 -0.264 N Y 1 Periplasmic Cj0983 Surface-Exposed Lipoprotein JlpA Y983_CAMJE 169 4 5.01 / 42164 -0.377 Y Y 2 Lipoprotein Cj0989 Putative Membrane Protein Q46118_CAMJE 65 2 9.45 / 8188 0.135 N N 1 Integral membrane

Cj0990c Hypothetical Protein Y990_CAMJE 69 3 5.29 / 29783 -0.481 N N 0 Cytoplasmic Cj0995c Delta-Aminolevulinic Acid Dehydratase (Porphobilinogen Synthase) [HemB] Q9PNU5_CAMJE 73 3 5.45 / 36422 -0.103 N N 0 Cytoplasmic Cj1001 RNA Polymerase Sigma Factor Sigma 70 [RpoD] Q9PNT9_CAMJE 46 3 5.54 / 72606 -0.787 N N 0 Cytoplasmic

Cj1002c Hypothetical Protein Phosphohistidine Phosphatase SixA Q9PNT8_CAMJE 58 1 8.57 / 19723 -0.333 N N 1 Cytoplasmic Cj1005c Putative Membrane-Bound ATPase [Cell Division Protein FtsH] Q9PNT5_CAMJE 89 4 8.38 / 61282 -0.140 N Y 2 Integral membrane Cj1007c Putative Mechanosensitive Ion Channel Family Protein Q9PNT3_CAMJE 306 13 8.74 / 60860 -0.095 N Y 5 Integral membrane Cj1013c Putative Membrane Protein / Cytochrome C Biogenesis Protein CcmF/CycK/CcsA Family Q9PNS7_CAMJE 435 11 8.63 / 123469 0.125 N Y 14 Integral membrane Cj1015c Branched-Chain Amino Acid ABC Transport ATP-Binding Protein [LivG] Q9PNS5_CAMJE 123 3 7.74 / 28718 -0.029 N N 0 Inner membrane Cj1024c Sigma-54-Associated Transcriptional Activator Q9PNR6_CAMJE 168 3 5.30 / 49232 -0.395 N N 0 Cytoplasmic Cj1026c Putative Lipoprotein Q9PNR4_CAMJE 600 6 7.74 / 18524 -0.108 Y Y 1 Lipoprotein Cj1029c Outer Membrane Lipoprotein MapA [MapA] MAPA_CAMJE 418 7 9.06 / 24040 -0.084 Y Y 1 Lipoprotein Cj1031 Putative Outer Membrane Component of Efflux System Q9PNR0_CAMJE 89 5 6.04 / 48970 -0.420 N N 1 Outer membrane Cj1033 Putative Integral Membrane Component of Efflux System Q9PNQ8_CAMJE 69 9 8.46 / 111685 0.318 N Y 11 Integral membrane

Cj1046c Putative Molybdopterin Biosynthesis Protein ThiF Q9PNP5_CAMJE 61 1 5.82 / 29652 -0.165 N N 1 Cytoplasmic Cj1054c UDP-N-Acetylmuramate--L-Alanine Ligase [MurC] MURC_CAMJE 100 5 6.06 / 48371 -0.155 N N 3 Integral membrane Cj1062 Putative CinA-Like Protein Q9PNM9_CAMJE 52 2 5.72 / 41047 -0.005 N N 0 Unknown Cj1069 Conserved Hypothetical Protein Q9PNM5_CAMJE 49 3 9.48 / 34645 -0.522 N N 0 Cytoplasmic

Cj1073c ATP-Dependant Protease La [Lon] LON_CAMJE 498 14 8.32 / 90099 -0.341 N N 0 Cytoplasmic Cj1082c Phosphomethylpyrimidine Kinase [ThiD] Q9PNL2_CAMJE 92 3 6.18 / 29248 0.047 N N 1 Cytoplasmic

Cj1084c Putative ATP/GTP-Binding Protein Q9PNL0_CAMJE 52 1 5.83 / 29396 -0.409 N N 0 Unknown Cj1087c Putative Peptidase Q9PNK7_CAMJE 120 2 9.11 / 34352 -0.305 N N 1 Periplasmic Cj1090c Putative Lipoprotein Q9PNK4_CAMJE 94 3 7.66 / 19004 -0.086 Y Y 1 Lipoprotein Cj1092c Protein-Export Membrane Protein SecF Q9PNK2_CAMJE 320 4 6.78 / 36112 0.311 N Y 6 Integral membrane Cj1093c Protein-Export Membrane Protein SecD Q9PNK1_CAMJE 480 7 7.72 / 57251 0.195 N Y 4 Integral membrane Cj1094c Putative Membrane Protein / Preprotein Translocase YajC Subunit Q9PNK0_CAMJE 304 3 8.76 / 10172 0.084 N Y 1 Inner membrane Cj1096c S-Adenosylmethionine Synthetase [MetK] Q9PNJ8_CAMJE 581 13 5.45 / 43654 -0.237 N N 0 Cytoplasmic Cj1101 ATP-Dependent DNA Helicase Q9PNJ3_CAMJE 238 4 6.15 / 79789 -0.417 N N 0 Cytoplasmic Cj1108 ATP-Dependant Clp Protease ATP-Binding Subunit ClpA Q9PNI6_CAMJE 78 5 6.80 / 80414 -0.329 N N 0 Cytoplasmic

Cj1110c Putative MCP-Type Signal Transduction Protein Q9PNI4_CAMJE 699 9 6.00 / 48350 -0.490 N N 0 Inner membrane Cj1115c Putative Phosphatidylserine Decarboxylase-Related Protein Q9PNH9_CAMJE 51 2 9.00 / 23235 0.363 N N 1 Integral membrane Cj1116c Membrane-Bound Zinc Metallopeptidase [FtsH Cell Division Protein] Q9PNH8_CAMJE 782 15 5.63 / 71048 -0.197 N N 3 Integral membrane Cj1119c Putative Integral Membrane Protein WlaM Q9PNH5_CAMJE 67 2 6.90 / 33943 0.410 N N 4 Integral membrane Cj1120c UDP-GlcNAc C4,6 Dehydratase [PglF] Q9PNH4_CAMJE 624 13 7.83 / 66765 0.211 N Y 4 Integral membrane Cj1125c GalNac Transferase PglA / WlaG Q9PNG9_CAMJE 73 2 9.20 / 42816 0.021 N N 0 Cytoplasmic Cj1129c GalNAc Transferase / Polymerase PglH [WlaC] Q9PNG5_CAMJE 70 2 8.66 / 41162 -0.085 N N 1 Cytoplasmic Cj1130c N-Linked Flippase PglK [WlaB] Q9PNG4_CAMJE 58 2 8.99 / 64739 0.196 N Y 6 Integral membrane Cj1135 Putative Two-Domain Glucosyltransferase Q7BC56_CAMJE 169 4 9.32 / 60490 -0.399 N N 2 Cytoplasmic

Cj1144c Hypothetical Protein * Q9PNE9_CAMJE 71 2 8.71 / 12367 -0.457 N N 0 Unknown Cj1155c Putative Cation-Transporting ATPase Q9PNE0_CAMJE 57 2 7.68 / 88480 0.174 N N 7 Integral membrane Cj1157 DNA Polymerase III Gamma / Tau Subunits [DnaX] Q9PND8_CAMJE 267 4 5.68 / 57960 -0.295 N N 0 Cytoplasmic Cj1170c 50kDa Outer Membrane Protein [Omp50] Q9PNC5_CAMJE 558 7 6.14 / 53339 -0.499 N Y 2 Outer membrane Cj1176c Sec-Independent Protein Translocase Protein TatA/E Homolog [TatA] TATA_CAMJE 223 3 6.56 / 8684 -0.506 N N 1 Inner membrane Cj1180c Putative ABC Transporter ATP-Binding Protein Q9PNB5_CAMJE 59 2 7.63 / 23642 -0.067 N N 0 Inner membrane Cj1182c 30S Ribosomal Protein S2 [RpsB] RS2_CAMJE 291 11 5.69 / 30225 -0.493 N N 0 Ribosomal Cj1184c Putative Ubiquinol Cytochrome C Reductase Cytochrome C Subunit (PetC) Q9PNB1_CAMJE 1153 12 5.85 / 41443 -0.166 N Y 2 Integral membrane Cj1185c Putative Ubiquinol Cytochrome C Reductase Cytochrome B Subunit (PetB) Q9PNB0_CAMJE 53 5 6.31 / 48096 0.696 N N 9 Integral membrane Cj1188c tRNA Uridine 5-Carboxymethylaminomethyl Modification Enzyme GIDA_CAMJE 61 2 6.64 / 69172 -0.227 N N 2 Cytoplasmic Cj1189c Bipartate Energy Taxis Response Protein CetB [Signal Tranduction Sensor Protein] Q9PNA6_CAMJE 210 6 7.72 / 19313 -0.570 N N 0 Cytoplasmic Cj1190c Bipartate Energy Taxis Response Protein CetA [Signal Tranduction Sensor Protein] Q9PNA5_CAMJE 1952 20 5.27 / 51016 -0.068 N Y 1 Inner membrane Cj1192 Putative C4-Dicarboxylate Transport Protein [DctA] Q9PNA3_CAMJE 99 1 8.49 / 49642 0.804 N N 10 Integral membrane

Cj1205c Putative DNA Repair Protein RadA Homolog [RadA] RADA_CAMJE 53 1 6.59 / 49024 0.025 N N 1 Unknown Cj1206c Putative Signal Recognition Particle Protein FtsY Q9PN89_CAMJE 45 4 5.64 / 32052 -0.135 N N 0 Cytoplasmic Cj1207c Putative Lipoprotein Thioredoxin Q9PN88_CAMJE 219 3 5.24 / 20959 -0.136 Y Y 3 Lipoprotein Cj1209 HD/KH-Domain Containing Protein UPF0144 Y1209_CAMJE 316 12 6.02 / 58025 -0.352 N N 1 Inner membrane

Cj1213c Putative Glycolate Oxidase Subunit D [GlcD] Q9PN82_CAMJE 305 7 6.50 / 50651 -0.140 N N 2 Unknown Cj1219c Putative Periplasmic Protein Q9PN76_CAMJE 296 10 9.17 / 92232 -0.124 N Y 2 Periplasmic Cj1221 60kDa Chaperonin GroEL CH60_CAMJE 310 5 5.02 / 57970 -0.117 N N 1 Cytoplasmic

Cj1226c Putative Two-Component Sensor Histidine Kinase Q9PN71_CAMJE 164 3 6.88 / 48526 -0.095 N N 2 Inner membrane Cj1227c DNA-Binding Response Regulator OmpR Q9PN70_CAMJE 272 4 5.63 / 25477 -0.329 N N 0 Cytoplasmic Cj1237c Putative Phosphatase Ppx / GppA Family Q9PN60_CAMJE 102 2 9.63 / 36799 -0.177 N N 0 Cytoplasmic Cj1243 Uroporphyrinogen Decarboxylase [HemE] DCUP_CAMJE 168 5 7.56 / 38922 -0.224 N N 2 Cytoplasmic Cj1244 Putative Radical SAM Domain-Containing Protein Q9PN53_CAMJE 64 3 5.97 / 34423 -0.218 N N 0 Cytoplasmic Cj1245c Putative Membrane Protein Q9PN52_CAMJE 65 4 7.59 / 46857 -0.161 N N 2 Integral membrane Cj1248 GMP Synthase GUAA_CAMJE 519 12 6.08 / 56976 -0.105 N N 2 Cytoplasmic Cj1249 Hypothetical Protein Q9PN48_CAMJE 67 5 8.92 / 56833 -0.479 N N 0 Unknown Cj1250 Phosphoribosylamine-Glycine Ligase PUR2_CAMJE 68 4 7.50 / 45315 -0.051 N N 1 Unknown Cj1252 Putative Periplasmic Protein Q9PN45_CAMJE 196 5 5.27 / 80440 -0.591 N Y 1 Periplasmic

Cj1256c Putative Membrane Protein Q9PN41_CAMJE 80 2 5.85 / 25488 -0.079 N Y 1 Integral membrane Cj1258 Putative Phosphotyrosine Protein Phosphatase Q9PN39_CAMJE 42 2 8.28 / 17289 -0.392 N N 0 Cytoplasmic Cj1259 Major Outer Membrane Protein (PorA) PORA_CAMJE 3532 13 4.71 / 45687 -0.179 N Y 2 Outer membrane

Cj1260c Chaperone Protein DnaJ DNAJ_CAMJE 49 2 6.12 / 41522 -0.619 N N 0 Cytoplasmic Cj1261 Two-Component Response Regulator RacR O68795_CAMJE 232 8 6.45 / 25398 -0.311 N N 0 Cytoplasmic

Cj1265c Ni/Fe Hydrogenase B-Type Cytochrome Subunit [HydC] Q9PN33_CAMJE 83 2 9.34 / 26797 0.398 N N 4 Integral membrane Cj1266c Ni/Fe Hydrogenase Large Subunit Q9PN32_CAMJE 937 14 6.26 / 63570 -0.234 N N 1 Unknown Cj1267c Ni/Fe Hydrogenase Small Subunit [HydA] Q9PN31_CAMJE 116 2 5.92 / 41438 -0.146 N N 3 Periplasmic Cj1270c Hypothetical Protein [Oxidoreductase 2-Nitropropane Dioxygenase Family] Q9PN28_CAMJE 525 10 8.38 / 39393 -0.134 N N 1 Cytoplasmic Cj1271c Tyrosyl-tRNA Synthetase [TyrS] Q9PN27_CAMJE 45 2 7.03 / 45420 -0.396 N N 0 Cytoplasmic Cj1272c Putative Guanosine-3'-5-Bis(Diphosphate) 3'-Pyrophosphohydrolase Q9PN26_CAMJE 53 2 6.45 / 83793 -0.171 N N 1 Cytoplasmic Cj1274c Uridylate Kinase [PyrH] PYRH_CAMJE 248 3 5.38 / 26019 0.022 N N 0 Cytoplasmic Cj1276c Putative Integral Membrane Protein Q9PN22_CAMJE 93 2 8.91 / 31507 0.685 N Y 4 Integral membrane Cj1277c Putative ABC Transporter ATP-Binding Protein Q9PN21_CAMJE 62 4 8.93 / 24954 -0.165 N N 0 Inner membrane Cj1278c Putative tRNA-Guanine-N(7)-Methyltransferase [TrmB] TRMB_CAMJE 110 2 8.98 / 46184 -0.418 N N 0 Cytoplasmic Cj1279c Putative Fibronectin-Domain Containing Lipoprotein Q9PN19_CAMJE 819 7 6.12 / 46124 -0.489 Y Y 1 Lipoprotein Cj1290c Biotin Carboxylase [AccC] Q9PN09_CAMJE 381 4 6.01 / 49091 -0.216 N N 0 Cytoplasmic Cj1292 Putative Deoxycytidine Triphosphate Deaminase [Dcd] DCD_CAMJE 183 4 6.15 / 20698 -0.087 N N 0 Cytoplasmic Cj1293 UDP-GlcNAc-Specific C4,6 Dehydratase / C5 Epimerase Q9PN06_CAMJE 49 1 8.41 / 37371 -0.219 N N 0 Flagellum Cj1305c Hypothetical Protein (617 Family) Q9PMZ4_CAMJE 88 5 6.64 / 47315 -0.398 N N 2 Unknown Cj1306c Hypothetical Protein (617 Family) Q9PMZ3_CAMJE 102 5 6.52 / 47256 -0.293 N N 2 Cytoplasmic Cj1307 Putative Amino Acid Adenylation-Domain Protein Q9PMZ2_CAMJE 162 7 6.14 / 57944 -0.165 N N 3 Cytoplasmic

Cj1310c Hypothetical Protein (617 Family) Q9PMY9_CAMJE 46 3 5.50 / 46620 -0.271 N N 3 Integral membrane Cj1312 Nucleotidase Specific for PseC Product UDP-4-Amino-4,6-Dideoxy-Beta-L-AltNAc [PseG] Q9PMY7_CAMJE 60 1 5.94 / 31319 -0.170 N N 0 Flagellum Cj1315c Imidazole Glycerol Phosphate Synthase Subunit HisH1 HIS51_CAMJE 45 1 7.68 / 22698 -0.087 N N 0 Cytoplasmic Cj1329 Putative Sugar-Phosphate Nucleotide Transferase QPMX0_CAMJE 58 1 9.39 / 38838 -0.244 N N 0 Cytoplasmic Cj1331 Acylneuraminate Cytidyltransferase [Flagellin Modification Protein PtmB] Q9PMW8_CAMJE 365 3 7.62 / 26660 -0.283 N N 0 Flagellum

Cj1340c Conserved Hypothetical Protein (1318 Family) Q9PMV9_CAMJE 140 7 5.67 / 70091 -0.149 N N 3 Unknown

Cj1347c Phosphatidate Cytidyltransferase [CdsA] Q9PMV2_CAMJE 82 2 7.66 / 26184 1.277 N Y 5 Integral membrane Cj1356c Putative Integral Membrane Protein Q9PMU3_CAMJE 104 2 9.61 / 20568 0.310 N Y 3 Integral membrane Cj1357c Putative Periplasmic Cytochrome c Q9PMU2_CAMJE 237 4 6.34 / 69224 -0.597 N Y 1 Periplasmic Cj1365c Putative Secreted Serine Protease Q9PMT5_CAMJE 58 5 7.04 / 116304 -0.322 N N 3 Secreted Cj1366c Glucosamine-Fructose-6-Phosphate Aminotransferase [GlmS] GLMS_CAMJE 272 10 5.76 / 67057 -0.126 N N 1 Cytoplasmic Cj1367c Putative Nucleotidyltransferase Q9PMT3_CAMJE 58 1 7.83 / 91631 -0.106 N N 1 Inner membrane Cj1368 Hypothetical Protein [Radical SAM Domain-Containing Protein] Q9PMT2_CAMJE 73 8 8.43 / 41277 -0.547 N N 0 Unknown Cj1370 Putative Nucleotide Phosphoribosyltransferase Q9PMT0_CAMJE 445 6 5.26 / 17034 -0.098 N N 0 Cytoplasmic

Cj1377c Putative Ferredoxin Q9PMS3_CAMJE 49 1 5.11 / 63066 -0.218 N N 4 Unknown Cj1379 Putative Selenocysteine-Specific Elongation Factor [SelB] Q9PMS1_CAMJE 50 6 8.62 / 69479 -0.287 N N 1 Cytoplasmic Cj1399c Putative Ni/Fe Hydrogenase Small Subunit [HydA2] Q9PMQ8_CAMJE 84 1 5.24 / 55973 -0.120 N N 3 Periplasmic Cj1402c Phosphoglycerate Kinase [Pgk] PGK_CAMJE 288 8 6.07 / 43591 -0.061 N N 0 Cytoplasmic Cj1408 Putative Flagellar Protein [FliL] Q9PMP9_CAMJE 157 6 4.93 / 19848 -0.137 N N 1 Flagellum

Cj1416c Putative Sugar Nucleotidyltransferase Q9PMP1_CAMJE 85 2 4.83 / 29533 -0.157 N N 1 Unknown Cj1419c Putative Methyltransferase Q9PMN8_CAMJE 56 5 5.92 / 29704 -0.646 N N 0 Cytoplasmic Cj1425c Putative D-Glycero-D-Manno-Heptose 7-Phosphate Kinase [HddA] Q6EF63_CAMJE 688 10 6.27 / 38100 -0.295 N N 2 Unknown Cj1428c GDP-L-Fucose Synthetase [Fcl] Q9PMM9_CAMJE 73 2 8.91 / 39431 -0.288 N N 0 Unknown Cj1430c Putative dTDP-4-Dehydrorhamnose 3,5-Epimerase Q9PMM7_CAMJE 54 2 6.52 / 21144 -0.422 N N 0 Cytoplasmic Cj1444c Capsular Polysaccharide Export System Periplasmic Protein [KpsD] Q9PML3_CAMJE 674 14 8.61 / 60841 -0.048 N Y 2 Periplasmic Cj1445c Putative Capsule Polysaccharide Export System Inner Membrane Protein KpsE Q9PML2_CAMJE 138 5 6.58 / 42517 -0.180 N N 2 Inner membrane Cj1447c Putative Capsule Polysaccharide Export ATP-Binding Protein [KpsT] O69286_CAMJE 58 1 8.84 / 24727 -0.195 N N 1 Inner membrane Cj1454c Putative Radical SAM Domain Family Protein Q9PMK6_CAMJE 90 6 5.44 / 49860 -0.212 N N 0 Cytoplasmic Cj1455 Peptide Chain Release Factor 2 [PrfB] RF2_CAMJE 88 4 4.97 / 41067 -0.545 N N 0 Cytoplasmic Cj1462 Flagellar P-Ring Protein [FlgI] FLGI_CAMJE 63 2 5.59 / 37040 -0.026 N Y 1 Periplasmic

Cj1476c Pyruvate-Flavodoxin Oxidoreductase Q9PMI6_CAMJE 8024 42 5.91 / 131357 -0.290 N N 2 Inner membrane Cj1478c Outer Membrane Fibronectin-Binding Protein CadF Q9PMI4_CAMJE 1916 14 5.89 / 35997 -0.675 N Y 1 Outer membrane Cj1479c 30S Ribosomal Protein S9 [RpsI] RS9_CAMJE 374 4 10.92 / 14136 -0.484 N N 0 Ribosomal Cj1483c Putative Lipoprotein Q9PMH9_CAMJE 189 4 8.82 / 20255 -0.277 N Y 1 Lipoprotein? Cj1484c Putative Membrane Protein Q9PMH8_CAMJE 44 1 9.52 / 22387 0.208 N Y 2 Integral membrane Cj1487c Cb-Type Cytochrome C Oxidase Subunit III [CcoP] Q9PMH5_CAMJE 266 3 5.07 / 31161 -0.003 N N 2 Inner membrane Cj1489c Cb-Type Cytochrome C Oxidase Subunit II [CcoO] Q9PMH3_CAMJE 583 6 5.86 / 24949 -0.273 N Y 1 Inner membrane Cj1491c Putative Two-Component Regulator Q9PMH1_CAMJE 185 6 5.90 / 25799 -0.085 N N 0 Cytoplasmic Cj1494c Carbamoyl-Phosphate Synthase Small Chain [CarA] CARA_CAMJE 362 6 6.16 / 42094 -0.361 N N 0 Cytoplasmic Cj1498c Adenylosuccinate Synthetase [PurA] PURA_CAMJE 124 10 5.72 / 46123 -0.242 N N 1 Cytoplasmic Cj1500 Putative Integral Membrane Protein Q9PMG3_CAMJE 138 6 9.72 / 44928 0.690 N Y 10 Integral membrane

Cj1502c Sodium / Proline Transporter [PutP] Q9PMG1_CAMJE 72 3 8.66 / 54209 0.840 N N 13 Integral membrane Cj1503c Putative Proline Dehydrogenase / Delta-1-Pyrroline-5-Carboxylate Dehydrogenase Q9PMG0_CAMJE 1484 31 6.04 / 132577 -0.287 N N 2 Cytoplasmic Cj1506c Putative MCP-Type Signal Transduction Protein Q9PMF7_CAMJE 1203 16 5.02 / 77268 -0.125 N Y 3 Inner membrane

Cj1507c Putative Regulatory Protein Q9PMF6_CAMJE 116 8 9.36 / 28337 -0.661 N N 0 Cytoplasmic Cj1515c Putative Decarboxylase [NspC] Q9PME9_CAMJE 58 1 6.11 / 43116 -0.207 N N 0 Cytoplasmic Cj1523c Putative CRISPR-Associated Protein Q9PME1_CAMJE 58 7 9.37 / 114896 -0.678 N N 2 Cytoplasmic Cj1529c Phosphoribosylformylglycinamidine Cyclo-Ligase [PurM] Q9PME0_CAMJE 74 4 5.15 / 35643 0.166 N N 1 Cytoplasmic Cj1531 Diaminopimelate Epimerase [DapF] DAPF_CAMJE 52 1 7.54 / 28410 -0.429 N N 1 Cytoplasmic

Cj1534c Putative Bacterioferritin Q9PMD5_CAMJE 318 2 5.55 / 17204 -0.333 N N 0 Cytoplasmic Cj1535c Glucose-6-Phosphate Isomerase [PGI] G6PI_CAMJE 355 8 6.36 / 46013 -0.031 N N 0 Cytoplasmic Cj1537c Acetyl-Coenzyme A Synthetase [AcsA] ACSA_CAMJE 225 6 5.59 / 73957 -0.281 N N 4 Cytoplasmic Cj1543 Putative Allophanate Hydrolase Subunit 2 Q9PMC6_CAMJE 74 3 5.93 / 35556 -0.215 N N 0 Cytoplasmic

Cj1553c Putative Type I Restriction Enzyme M Protein HsdM Q7B7V5_CAMJE 75 2 5.33 / 56789 -0.433 N N 1 Cytoplasmic Cj1560 Putative Membrane Protein Permease Q7AR72_CAMJE 121 1 9.65 / 30739 0.872 N N 6 Integral membrane Cj1564 Methyl-Accepting Chemotaxis Protein 4962 23 5.47 / 73121 -0.105 N Y 3 Integral membrane

Cj1567c NADH Dehydrogenase I Chain M [NuoM] Q9PMA8_CAMJE 185 2 8.78 / 55832 1.179 N Y 13 Integral membrane Cj1571c NADH Dehydrogenase I Chain I [NuoI] Q9PMA4_CAMJE 65 2 8.64 / 24971 -0.605 N N 0 Cytoplasmic Cj1572c NADH Dehydrogenase I Chain H [NuoH] Q9PMA3_CAMJE 63 2 5.54 / 36707 1.055 N N 8 Integral membrane Cj1573c Probable NADH Dehydrogenase I Chain G [NuoG] Q9PMA2_CAMJE 54 2 5.49 / 93331 -0.418 N N 1 Unknown Cj1574c Hypothetical Protein Q9PMA1_CAMJE 187 6 4.94 / 26373 -0.231 N N 0 Unknown Cj1585c Putative Oxidoreductase FAD-Binding Iron-Sulfur Cluster Protein Q9PM90_CAMJE 331 10 8.53 / 103910 -0.186 N N 3 Cytoplasmic Cj1592 30S Ribosomal Protein S13 [RpsM] RS13_CAMJE 56 1 10.28 / 13735 -0.655 N N 0 Ribosomal Cj1593 30S Ribosomal Protein S11 [RpsK] RS11_CAMJE 163 4 10.29 / 13893 -0.345 N N 0 Ribosomal Cj1594 30S Ribosomal Protein S4 [RpsD] RS4_CAMJE 298 8 10.34 / 23909 -0.519 N N 0 Ribosomal Cj1595 DNA-Directed RNA Polymerase Alpha Chain [RpoA] RPOA_CAMJE 298 6 4.97 / 37686 -0.195 N N 0 Cytoplasmic Cj1596 50S Ribosomal Protein L17 [RplQ] Q9PM79_CAMJE 212 4 11.00 / 13248 -0.480 N N 0 Ribosomal

Cj1605c Putative 2,3,4,5-Tetrahydropyridine-2-Carboxylate N-Succinyltransferase [DapD] Q9PM70_CAMJE 198 6 5.77 / 42360 -0.007 N N 2 Cytoplasmic Cj1606c Putative ATP/GTP-Binding Protein Homolog (Mpr) Q9PM69_CAMJE 48 4 5.48 / 40119 -0.101 N N 1 Unknown Cj1611 30S Ribosomal Protein S20 [RpsT] RS20_CAMJE 244 1 11.94 / 9816 -0.757 N N 0 Ribosomal Cj1619 Alpha-Ketoglutarate Permease [KgtP] Q9PM56_CAMJE 42 2 9.43 / 46030 0.684 N N 12 Integral membrane Cj1621 Putative Periplasmic Protein Q9PM54_CAMJE 526 5 8.15 / 28334 -0.323 N Y 1 Periplasmic

Cj1624c L-Serine Dehydratase [SdaA] Q9PM51_CAMJE 188 8 5.94 / 49507 -0.107 N N 4 Integral membrane Cj1625c Serine Transporter [SdaC] Q9PM50_CAMJE 419 5 8.87 / 46112 0.985 N Y 10 Integral membrane Cj1633 Putative ATP-Binding Protein Q9PM42_CAMJE 42 1 7.60 / 36755 -0.234 N N 0 Unknown Cj1638 DNA Primase [DnaG] PRIM_CAMJE 58 4 8.61 / 69055 -0.260 N N 0 Cytoplasmic Cj1645 Transketolase [Tkt] Q9PM31_CAMJE 56 2 6.05 / 69636 -0.198 N N 0 Cytoplasmic Cj1658 Putative Iron Permease Q9PM19_CAMJE 46 3 5.29 / 77817 0.117 N Y 7 Integral membrane Cj1663 Putative ABC Transport System ATP-Binding Protein Q9PM14_CAMJE 48 2 5.42 / 24459 -0.278 N N 0 Inner membrane

Cj1673c RecA Protein RECA_CAMJE 208 5 5.23 / 37049 -0.248 N N 1 Cytoplasmic Cj1689c 50S Ribosomal Protein L15 [RplO] Q9PLY9_CAMJE 161 5 10.46 / 14027 -0.684 N N 0 Ribosomal Cj1690c 30S Ribosomal Protein S5 [RpsE] RS5_CAMJE 376 5 9.95 / 15796 -0.080 N N 0 Ribosomal

Cj1691c 50S Ribosomal Protein L18 [RplR] Q5HSA6_CAMJE 348 6 10.67 / 13266 -0.281 N N 0 Ribosomal Cj1692c 50S Ribosomal Protein L6 [RplF] Q9PLY6_CAMJE 172 4 9.76 / 19586 -0.413 N N 0 Ribosomal Cj1693c 30S Ribosomal Protein S8 [RpsH] RS8_CAMJE 76 3 9.59 / 14733 -0.280 N N 0 Ribosomal Cj1695c 50S Ribosomal Protein L5 [RplE] RL5_CAMJE 100 5 9.67 / 20213 -0.113 N N 0 Ribosomal Cj1697c 50S Ribosomal Protein L14 [RplN] Q9PLY1_CAMJE 418 6 10.28 / 13305 -0.027 N N 0 Ribosomal Cj1698c 30S Ribosomal Protein S17 [RpsQ] Q9PLY0_CAMJE 184 5 10.37 / 9549 -0.278 N N 0 Ribosomal Cj1699 50S Ribosomal Protein L29 [RpmC] RL29_CAMJE 54 1 10.06 / 7033 -0.423 N N 0 Ribosomal

Cj1700c 50S Ribosomal Protein L16 [RplP] Q9PLX8_CAMJE 71 2 10.54 / 16375 -0.648 N N 0 Ribosomal Cj1701c 30S Ribosomal Protein S3 [RpsC] RS3_CAMJE 567 11 10.45 / 26063 -0.508 N N 0 Ribosomal Cj1702c 50S Ribosomal Protein L22 [RplV] RL22_CAMJE 183 3 10.74 / 15243 -0.334 N N 0 Ribosomal Cj1704c 50S Ribosomal Protein L2 [RplB] RL2_CAMJE 401 10 10.36 / 30420 -0.654 N N 0 Ribosomal Cj1706c 50S Ribosomal Protein L4 [RplD] RL4_CAMJE 349 7 9.73 / 22229 -0.288 N N 0 Ribosomal Cj1707c 50S Ribosomal Protein L3 [RplC] RL3_CAMJE 370 4 9.91 / 20736 -0.431 N N 0 Ribosomal Cj1713 Putative Radical SAM Domain-Containing Protein Q9PLW5_CAMJE 69 2 9.28 / 40254 -0.245 N N 0 Cytoplasmic Cj1721c Probable Outer Membrane Protein Q9PLV7_CAMJE 119 4 8.99 / 24029 -0.161 N Y 1 Outer membrane Cj1729c Flagellar Hook Subunit Protein FlgE Q9PLU9_CAMJE 75 3 4.86 / 91918 -0.422 N N 0 Flagellum CJE1284 Putative Lipooligosaccharide Biosynthesis Glycosyltransferase Q5HTW3_CAMJR 94 3 9.18 / 30789 -0.308 N N 0 Cytoplasmic CJE1724 Type I Restriction-Modification System M Subunit HsdM Q5HSN6_CAMJR 50 2 6.06 / 56741 -0.405 N N 1 Cytoplasmic

Supplementary Table 2. Identification of proteins from C. jejuni JHH1 membrane protein-enriched fractions separated by 2-DLC and identified by tandem-MS Shaded boxes represent proteins also identified in the 2-DE data set and shown in Supplementary Table 1. ? Cj1483c contains a predicted SP by SignalP, but no SP by LipoP analysis, therefore lipoprotein status is questionable