supplementary file 1 supplementary figure s1 page 2 · direct go count (cc) [fusca] 0 500 1,000...

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Supplementary File 1

Supplementary Figure S1 ………………………………………………………………… Page 2







a) b)

2

Supplementary Figure S1. Formica fusca larva eating an egg. a) before eating the egg b) after eating the egg.

a) b)

3

Supplementary Figure S 2. Size of Formica fusca larvae used in this study. a) eggs in the top row, pupae in the bottom row, and larvae of increasing size in between b) magnified view of the upper four rows, the bottom two rows representing the larvae used in this study.

Number of GO-terms for Sequences with Length(x) [fusca]

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#GO

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ber G

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rms

4Supplementary Figure S3. Relationship between the length of the unigenes and the number of GOterms associated.

GO-Level Distr ibution [fusca]

P F C

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

GO Level (Total Annotations = 34402, Mean Level = 6.36, Std. Deviation = 2.528)

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Biological processMolecular functionCellular components

GO Level

Num

ber o

f ann

otat

ions

5

Supplementary Figure S4. Distribution of hierarchical level of GO annotations. For each annotation level, the number of sequences mapping to a term is shown for biological process, molecular function and cellular components. Total annotation = 34402, Mean level of GOterm = 6.36, Deviation = 2.53.

Direct GO Count (BP) [fusca]

0 500 1,000 1,500 2,000 2,500 3,000 3,500 4,000 4,500 5,000#Seqs

oxidation-reduction processproteolysis

regulation of transcription, DNA-templatedtransmembrane transport

signal transductionDNA integration

protein phosphorylationphosphorylation

nucleic acid phosphodiester bond hydrolysismetabolic process

protein ubiquitinationG-protein coupled receptor signaling pathway

translationintracellular signal transductioncarbohydrate metabolic process

ubiquitin-dependent protein catabolic processdetection of chemical stimulus involved ...

microtubule-based movementmethylation

peptidyl-tyrosine phosphorylationbiosynthetic process

DNA repairintracellular protein transport

DNA replicationRNA-dependent DNA biosynthetic process

lipid metabolic processpositive regulation of GTPase activity

translational initiationcell redox homeostasis

multicellular organism developmentchitin metabolic process

DNA recombinationnitrogen compound metabolic process

primary metabolic processRNA processing

transposition, DNA-mediatedDNA metabolic process

regulation of Rho protein signal transductionion transmembrane transport

vesicle-mediated transportmacromolecule metabolic process

protein foldingprotein transport

response to stimuluspotassium ion transmembrane transport

regulation of transcription by RNA polymera...peptidyl-tyrosine dephosphorylation

transcription, DNA-templatedDNA biosynthetic process

others

GOG

o te

rms (

Biol

ogic

al p

roce

sses

)

Number of sequences

6Supplementary Figure S5. GO annotation classification of the top 50 biological processes.

Direct GO Count (MF) [fusca]

0 500 1,000 1,500 2,000 2,500 3,000 3,500 4,000 4,500 5,000 5,500#Seqs

ATP bindingnucleic acid binding

metal ion bindingzinc ion binding

DNA bindingRNA binding

calcium ion bindingtransferase activity

oxidoreductase activitykinase activityheme binding

sequence-specific DNA bindingprotein kinase activity

DNA-binding transcription factor activityiron ion binding

protein dimerization activityGTP binding

hydrolase activityligase activity

monooxygenase activitystructural constituent of ribosome

oxidoreductase activity, acting on pair...serine-type endopeptidase activity

bindingcatalytic activity

microtubule motor activityodorant binding

endonuclease activityolfactory receptor activity

transmembrane transporter activityATPase activity

nucleotide bindingGTPase activity

methyltransferase activityhelicase activity

protein serine/threonine kinase activityG-protein coupled receptor activity

actin bindingflavin adenine dinucleotide binding

microtubule bindingtranslation initiation factor activity

hydrolase activity, acting on ester bondsRNA-directed DNA polymerase activity

ubiquitin-protein transferase activityGTPase activator activity

aspartic-type endopeptidase activitypyridoxal phosphate bindingmetalloendopeptidase activity

chitin bindingothers

GO

Number of sequences

Go

term

s (M

olec

ular

func

tion)

7Supplementary Figure S6. GO annotation classification of the top 50 molecular functions.

Direct GO Count (CC) [fusca]

0 500 1,000 1,500 2,000 2,500 3,000 3,500#Seqs

integral component of membranenucleus

membranecytoplasm

intracellularplasma membrane

extracellular regionribosome

mitochondrionubiquitin ligase complex

cellendoplasmic reticulum membrane

dynein complexcytoskeleton

Golgi membranecytosol

microtubulemitochondrial inner membrane

nucleolusmyosin complex

endoplasmic reticulumextracellular space

motile ciliummediator complex

chromosomeperoxisome

integral component of plasma membranehistone acetyltransferase complex

Golgi apparatusproteasome complex

transcription factor complexchloroplast

collagen trimermicrotubule organizing center

spliceosomal complexpostsynapse

COPII vesicle coatmitochondrial matrix

mitochondrial outer membraneproton-transporting V-type ATPase, V1 domain

nuclear poreeukaryotic 43S preinitiation complex

cytosolic large ribosomal subuniteukaryotic 48S preinitiation complex

large ribosomal subunitmeiotic nuclear membrane microtubu...

transcription factor TFIID complexvoltage-gated potassium channel complex

centrosomeothers

GO

Number of sequences

Go

term

s (Ce

llula

r com

pone

nt)

8Supplementary Figure S7. GO annotation classification of the top 50 cellular components.

supplementary file 1 supplementary figure s1 page 2 · direct go count (cc) [fusca] 0 500 1,000...

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