Hennigar, S.R. 1

Curriculum Vitae

Stephen R. Hennigar, Ph.D.

Work address: Department of Nutrition, Food, and Exercise Sciences

College of Human Sciences

Florida State University

414 Sandels Building

120 Convocation Way

Tallahassee, FL 32306

Email address: hennigar.s.r@gmail.com

Phone number: 850-644-4792 (office)

603-828-3392 (cell)

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CURRENT POSITION

2018- Assistant Professor

Florida State University

Department of Nutrition, Food, and Exercise Sciences

Tallahassee, FL

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RESEARCH EXPERIENCE

2017-2018 Nutrition Biologist

United States Army Research Institute of Environmental Medicine

Military Nutrition Division

Natick, MA

2014-2017 Postdoctoral Fellow

United States Army Research Institute of Environmental Medicine

Military Nutrition Division

Natick, MA

Mentor: James P. McClung, Ph.D.

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EDUCATION

2010-2014 The Pennsylvania State University

Department of Nutritional Sciences

University Park, PA

Ph.D. in Nutritional Sciences

Dissertation Title: ZnT2 (SLC30A2)-mediated zinc transport in the post-

lactational involution of the mammary gland: regulation and dysregulation

Mentor: Shannon L. Kelleher, Ph.D.

Hennigar, S.R. 2

2008-2010 University of New Hampshire

Department of Molecular, Cellular, and Biomedical Sciences

Durham, NH

M.S. in Nutritional Sciences

Thesis Title: Exposure to polybrominated diphenyl ethers (PBDEs) suppresses the

release of pro-inflammatory products by alveolar macrophages in vitro

Mentor: Anthony R. Tagliaferro, Ph.D.

2004-2008 University of New Hampshire

Department of Molecular, Cellular, and Biomedical Sciences

Durham, NH

B.S. in Nutritional Sciences

Minor in Spanish

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PEER-REVIEWED PUBLICATIONS

Stephen R. Hennigar, Harris R. Lieberman, Victor L. Fulgoni, James P. McClung. Serum zinc

concentrations in the U.S. population are related to sex, age, and time of blood draw, but not

dietary or supplemental zinc. Journal of Nutrition, 148:1341-1351, 2018.

Stephen R. Hennigar, James P. McClung, and Stefan M. Pasiakos. Nutritional interventions and

the interleukin-6 response to exercise. FASEB J, 31:3719-3728, 2017.

Stephen R. Hennigar and James P. McClung. Hepcidin attenuates zinc transport in Caco-2

cells. Journal of Nutrition, 146:2167-2173, 2016.

Stephen R. Hennigar, Alyssa M. Kelley, and James P. McClung. Metallothionein and zinc

transporter expression in circulating blood cells as biomarkers of zinc status: a systematic

review. Advances in Nutrition, 7:735-46, 2016.

Stephen R. Hennigar*, Erin Gaffney-Stomberg*, Laura J. Lutz, Sonja J. Cable, Stefan M.

Pasiakos, Andrew J. Young, and James P. McClung. Consumption of a calcium and vitamin D

fortified food product does not affect iron status during initial military training: a randomized,

double-blind, placebo, controlled trial. British Journal of Nutrition, 2:1-7, 2015.

*equal contribution

Stephen R. Hennigar and James P. McClung. Homeostatic regulation of trace mineral transport

by ubiquitination of membrane transporters. Nutrition Reviews, 74:59-67, 2016.

Nicholas H. McCormick, Stephen R. Hennigar, and Shannon L. Kelleher. ZnT4 (SLC30A4)-

null (“lethal milk”) mice have defects in mammary gland secretion and hallmarks of precocious

involution during lactation. American Journal of Physiology – Regulatory, Integrative, and

Comparative Physiology, 310:R33-40, 2016.

Hennigar, S.R. 3

Stephen R. Hennigar, Vanessa Velasquez, and Shannon L. Kelleher. Obesity-induced

inflammation is associated with alterations in subcellular zinc pools and premature mammary

gland involution in lactating mice. Journal of Nutrition, 145:1999-2005, 2015.

Samina Alum, Stephen R. Hennigar, Carla Gallagher, David I. Soybel, and Shannon L.

Kelleher. Exome sequencing of SLC30A2 identifies novel loss- and gain-of-function variants

associated with breast cell dysfunction. Journal of Mammary Gland Biology and Neoplasia,

20:159-72, 2015.

Sooyeon Lee, Stephen R. Hennigar, Samina Alam, Keigo Nishida, and Shannon L. Kelleher.

Essential role of ZnT2-mediated zinc transport in mammary gland development and function

during lactation. Journal of Biological Chemistry, 290:13064-78, 2015.

Stephen R. Hennigar and Shannon L. Kelleher. TNFα post-translationally targets ZnT2 to

accumulate zinc in lysosomes. Journal of Cellular Physiology, 230:2345-50, 2015.

Stephen R. Hennigar, Young Ah Seo, Supriya Sharma, and Shannon L. Kelleher. ZnT2 is

critical mediator of lysosomal-mediated cell death during early mammary gland involution.

Scientific Reports, 5:8033, 2015.

Young Ah Seo, Sooyeon Lee, Stephen R. Hennigar, and Shannon L. Kelleher. Prolactin (PRL)-

stimulated ubiquitination of ZnT2 mediates a transient increase in zinc secretion followed by

ZnT2 degradation in mammary epithelial cells. Journal of Biological Chemistry, 289:23653-61,

2014.

Nicholas M. McCormick, Stephen R. Hennigar, Kirill Kiselyov, and Shannon L. Kelleher. The

biology of zinc transport in mammary epithelial cells: implications for mammary gland

development, lactation, and involution. Journal of Mammary Gland Biology and Neoplasia,

19:59-71, 2014.

Stephen R. Hennigar and Shannon L. Kelleher. Zinc networks: the cell-specific

compartmentalization of zinc for specialized functions. Biological Chemistry, 393:565-78, 2012.

Stephen R. Hennigar, Jay L. Myers, Anthony R. Tagliaferro. Exposure of alveolar macrophages

to polybrominated diphenyl ethers (PBDEs) suppresses the release of pro-inflammatory products

in vitro. Experimental Biology and Medicine, 237:429-34, 2012.

Manuscripts in press

Stephen R. Hennigar and James P. McClung. Zinc transport in the mammalian intestine.

*Invited Overview Article for Comprehensive Physiology.

Manuscripts in review

N/A

Hennigar, S.R. 4

Invited editorials

Stephen R. Hennigar and James P. McClung. Nutritional immunity: starving pathogens of trace

minerals. American Journal of Lifestyle Medicine, 10:170-3, 2016.

Published abstracts

Stephen R. Hennigar, Harris R. Lieberman, Victor L. Fulgoni III, James P. McClung. Factors

affecting serum zinc concentrations in the U.S. population: National Health and Nutrition

Examination Survey (2011-2014). Current Developments in Nutrition, in press.

Stephen R. Hennigar, Alyssa M. Kelley, Bradley J. Anderson, James P. McClung. Effects of

extracellular zinc on mRNA expression of zinc transporters and metallothioneins in peripheral

blood mononuclear cells. Current Developments in Nutrition, in press.

Alyssa M. Kelley, Stephen R. Hennigar, Anna T. Nakayama, Erin Gaffney-Stomberg, James P.

McClung. Dietary magnesium intake and serum magnesium concentration in military recruits:

relationship with indicators of bone structure and function. Current Developments in Nutrition,

in press.

Stephen R. Hennigar, J. Philip Karl, Alyssa M. Kelley, Bradley J. Anderson, Nicholes J.

Armstrong, Holly L. McClung, Claire E. Berryman, and James P. McClung. Metallothoinein-2A

mRNA in peripheral blood mononuclear cells reflects dietary zinc intake in healthy adults.

FASEB J. 2017 31:459.3

James P. McClung, Stephen R. Hennigar, Claire E. Berryman, Andrew J. Young, and Stefan

M. Pasiakos. Prolonged high altitude exposure results in elevated erythroferrone and diminished

hepcidin levels in healthy young male volunteers. FASEB J. 2017 31:637.2

Alyssa M. Kelley, Stephen R. Hennigar, and James P. McClung. Effects of hepcidin on zinc

homeostasis in HepG2 cells. FASEB J. 2017 31:802.4

Philip Marszewski, Stephen R. Hennigar, Alyssa M. Kelley, Bradley J. Anderson, James P.

McClung, and Erin Gaffney-Stomberg. Femur cortical bone mineral content is increased with

high protein intake during ad libitum but not energy restricted conditions in obese female Zucker

rats. FASEB J. 2017 31:643.19

Olivia C. Rivera, Stephen R. Hennigar, Sooyeon Lee, and Shannon L. Kelleher. ZnT2 regulates

lysosomal acidification and biogenesis during mammary gland involution. FASEB J 2017

31:448.4

Stephen R. Hennigar and James P. McClung. Hepcidin attenuates zinc efflux in Caco-2 cells.

FASEB J. 2016 30:292.3

Hennigar, S.R. 5

James P. McClung, Laura J. Lutz, Stephen R. Hennigar, J. Philip Karl, Sonya J. Cable, Thomas

L. Cropper, and Erin Gaffney-Stomberg. Associations between serum leptin, iron status, and

dietary iron intake in young adults. FASEB J. 2016 30:1173.6

Olivia C. Rivera, Stephen R. Hennigar, and Shannon L. Kelleher. Loss of ZnT2-mediated zinc

transport leads to cytoplasmic zinc accumulation and impairs mammary gland involution.

FASEB J. 2016 30:1224.14

Stephen R. Hennigar and Shannon L. Kelleher. TNFα post-translationally targets ZnT2 to

lysosomes to activate lysosomal-mediated cell death and mammary gland involution. FASEB J.

2015 29:263.7

Stephen R. Hennigar, Vanessa Velasquez, and Shannon L. Kelleher. Diet-induced obesity

compromises lactation through zinc-mediated ER stress and autophagy, promoting premature

involution. FASEB J. 2014 28:910.4

Shannon L. Kelleher, Stephen R. Hennigar, David I. Soybel, and Samina Alam. Genetic

variation in SLC30A2 (ZnT2): associations with mammary cell function and milk zinc

concentration in breastfeeding women. FASEB J. 2014 28:893.41

Stephen R. Hennigar and Shannon L. Kelleher. TNFα redistributes ZnT2 to accumulate zinc in

lysosomes and activate autophagic cell death in mammary epithelial cells. FASEB J. 2013

27:234.3

Sooyeon Lee, Stephen R. Hennigar, Samina Alam, and Shannon L. Kelleher. ZnT2-null mice

have distinct morphological defects in the mammary gland that impair development and

function. FASEB J. 2013 27:733.7

Stephen R. Hennigar, Supriya Sharma, Young Ah Seo, and Shannon L. Kelleher. ZnT2

accumulates zinc in lysosomes and mitochondria to signal mammary gland involution. FASEB J.

2012 26:390.7

Stephen R. Hennigar and Shannon L. Kelleher. Pro-inflammatory cytokines dysregulate

mammary gland zinc homeostasis. FASEB J. 2011 25:228.3

James P. McClung, Nancy E. Andersen, Bryan C. Wiley, Louis J. Marchitelli, Stephen R.

Hennigar, and Andrew J. Young. Iron deficiency does not cause obesity in adult rats. FASEB J.

2010 24:717.6

Stephen R. Hennigar, Anne M. Ronan, Jay L. Myers, and Anthony R. Tagliaferro. Exposure of

activated alveolar macrophages to brominated flame retardants augments the release of pro-

inflammatory cytokines in vitro. FASEB J. 2010 24:724.6

Stephen R. Hennigar, Adam C. Mirando, Anne M. Ronan, and Anthony R. Tagliaferro.

Exposure to flame retardants promotes increased consumption of simple sugars. FASEB J. 2008

22:888.7

Hennigar, S.R. 6

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RESEARCH PRESENTATIONS

Oral presentations

Department of Behavioral Health and Nutrition, University of Delaware, Newark, DE (2018):

Physiological regulators of mineral absorption and status.

Department of Nutrition, Food, and Exercise Sciences, Florida State University, Tallahassee, FL

(2017): Physiological regulators of mineral absorption and status.

Experimental Biology Meeting, Chicago, IL (2017): Metallothionein-2A mRNA in peripheral

blood mononuclear cells reflects dietary zinc intake in healthy adults.

Experimental Biology Meeting, San Diego, CA (2016): Hepcidin attenuates zinc efflux in Caco-

2 cells.

*Selected for Post-Doctoral Research Award

Department of Molecular, Cellular, and Biomedical Sciences Seminar Series, University of New

Hampshire, Durham, NH (2015): Effects of inflammation on zinc transport.

Experimental Biology Meeting, Boston, MA (2015): TNFα post-translationally targets ZnT2 to

lysosomes to activate lysosomal-mediated cell death and mammary gland involution.

International Society for Zinc Biology Meeting, Pacific Grove, CA (2014): TNF-alpha post-

translationally traffics ZnT2 to lysosomes to activate lysosomal-mediated cell death and

mammary gland involution.

*Selected Student Presentation

Doctoral Defense Seminar, University Park, PA (2014): ZnT2 (SLC30A2)-mediated zinc

transport in the post-lactational involution of the mammary gland: regulation and dysregulation.

U.S. Army Research Institute of Environmental Medicine, Natick, MA (2014): Inflammation

regulates zinc-mediated cell death: regulation and dysregulation in mouse models.

Experimental Biology Meeting, San Diego, CA (2014): Diet-induced obesity compromises

lactation through zinc-mediated ER stress and autophagy, promoting premature involution.

Experimental Biology Meeting, Boston, MA (2013): TNFα redistributes ZnT2 to accumulate

zinc in lysosomes and activate autophagic cell death in mammary epithelial cells.

Experimental Biology Meeting, San Diego, CA (2012): ZnT2 accumulates zinc in lysosomes and

mitochondria to signal mammary gland involution.

Penn State Nutritional Sciences Colloquium Series, University Park, PA (2012): The involuting

mammary gland: What’s zinc got to do with it?

Hennigar, S.R. 7

Experimental Biology Meeting, Washington, D.C. (2011): Pro-inflammatory cytokines regulate

mammary gland zinc homeostasis.

Master’s Thesis Defense, Durham, NH (2010): Exposure to polybrominated diphenyl ethers

(PBDEs) suppresses the release of pro-inflammatory products by alveolar macrophages in vitro.

NCR-97 Meeting, Anaheim, CA (2010): Exposure to polybrominated diphenyl ethers (PBDEs)

suppresses the release of pro-inflammatory products by alveolar macrophages in vitro.

Graduate Seminar, Durham, NH (2009): The effects of PBDEs on alveolar macrophage

activation in vitro.

Society of Environmental Toxicology and Chemistry Meeting, Durham, NH (2009):

Polybrominated diphenyl ethers (PBDEs): promoters of sweet preference, overeating, and weight

gain in male rats.

Graduate Seminar, Durham, NH (2009): Chronic exposure to PBDEs augments weight gain and

alters metabolic efficiency in male and female rats.

Poster presentations

Nutrition 2018, Boston, MA (2018): Factors affecting serum zinc concentrations in the U.S.

population: National Health and Nutrition Examination Survey (2011-2014)

Nutrition 2018, Boston, MA (2018): Effects of extracellular zinc on mRNA expression of zinc

transporters and metallothioneins in peripheral blood mononuclear cells

Emerging Leaders in Nutritional Sciences Poster Competition, San Diego, CA (2016): Hepcidin

attenuates zinc efflux in Caco-2 cells.

American Physiological Society Endocrinology & Metabolism Section Campbell Award Poster

Competition, San Diego, CA (2014): Diet-induced obesity compromises lactation through zinc-

mediated ER stress and autophagy, promoting premature involution.

Vitamins & Minerals Research Interest Section Poster Competition, San Diego, CA (2014): Diet-

induced obesity compromises lactation through zinc-mediated ER stress and autophagy,

promoting premature involution.

The Graduate Exhibition, University Park, PA (2014): Diet-induced obesity compromises

lactation through zinc-mediated ER stress and autophagy, promoting premature involution.

College of Health and Human Development Research Forum, University Park, PA (2014): Diet-

induced obesity compromises lactation through zinc-mediated ER stress and autophagy,

promoting premature involution.

Hennigar, S.R. 8

Vitamins & Minerals Research Interest Section Poster Competition, Boston, MA (2013): TNFα

redistributes ZnT2 to accumulate zinc in lysosomes and activate autophagic cell death in

mammary epithelial cells.

The Graduate Exhibition, University Park, PA (2013): TNFα redistributes ZnT2 to accumulate

zinc in lysosomes and activate autophagic cell death in mammary epithelial cells.

College of Health and Human Development Research Forum, University Park, PA (2013): TNFα

redistributes ZnT2 to accumulate zinc in lysosomes and activate autophagic cell death in

mammary epithelial cells.

Vitamins & Minerals Research Interest Section Poster Competition, San Diego, CA (2012):

ZnT2 accumulates zinc in lysosomes and mitochondria to signal mammary gland involution.

The Graduate Exhibition, University Park, PA (2011): Pro-inflammatory cytokines dysregulate

mammary gland zinc homeostasis.

Experimental Biology Meeting, Anaheim, CA (2010): Exposure of activated alveolar

macrophages to brominated flame retardants augments the release of pro-inflammatory cytokines

in vitro.

Graduate Research Conference, Durham, NH (2010): Exposure of activated alveolar

macrophages to brominated flame retardants augments the release of pro-inflammatory cytokines

in vitro.

Undergraduate Research Conference, Durham, NH (2008): Exposure to flame retardants

promotes increased consumption of simple sugars.

Experimental Biology Meeting, San Diego, CA (2008): Exposure to flame retardants promotes

increased consumption of simple sugars.

Undergraduate Research Conference, Durham, NH (2007): The potential link between the

exposure to environmental pollutants and preference for sweet-tasting fluids and obesity in rats.

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GRANTS

Funded

Funding Agency: Defense Health Program

Project Title: Effects of inflammation on mineral homeostasis in an animal model

Role: Principal Investigator

Award: $172,000

Date of Award: 2016

Hennigar, S.R. 9

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PROCEDURAL PRIVILEGES

Dual-energy X-ray absorptiometry

Peripheral quantitative computed tomography

Venipuncture

Venous catheter placement

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TEACHING

Instructor

Institution: Department of Nutrition, Food, and Exercise Sciences

Florida State University

Course Title: Intermediary Metabolism II (HUN 3226)

Date: Fall 2018

Institution: Department of Nutrition, Food, and Exercise Sciences

Florida State University

Course Title: Food and Nutrition Seminar (FOS 5930/6930 and HUN 5930/6930)

Date: Fall 2018

Institution: Department of Nutritional Sciences, Penn State University

Course Title: Introductory Principles of Nutrition (NUTR 251)

Date: 2012

Teaching assistant

Institution: Department of Nutritional Sciences, Penn State University

Course Title: Nutrient Metabolism II (NUTR 446)

Date: 2011-2013

Institution: Department of Molecular, Cellular, and Biomedical Sciences, University

of New Hampshire

Course Title: Nutrition Health & Well Being (NUTR 400)

Date: 2008-2010

Institution: Department of Molecular, Cellular, and Biomedical Sciences, University

of New Hampshire

Course Title: Nutritional Assessment (NUTR 476), 2010

Date: 2010

Invited lecturer

Hennigar, S.R. 10

Institution: Department of Molecular, Cellular, and Biomedical Sciences, University

of New Hampshire

Course: Seminar Series

Title of talk: Effects of inflammation on zinc transport

Date: 2015

Institution: Department of Nutritional Sciences, Penn State University

Course: Global Nutrition (NUTR 425)

Title of talk: (1) Micronutrients in breast milk and (2) Immune benefits of breast milk

Date: 2013

Institution: Department of Molecular, Cellular, and Biomedical Sciences, University

of New Hampshire

Course: Nutritional Biochemistry (NUTR 750)

Title of talk: Nutrition and exercise performance

Date: 2010

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MEMBERSHIP & SERVICE

Membership

American Society for Nutrition (ASN)

ASN Early Career Nutrition Interest Group

ASN Vitamins & Minerals Research Interest Section

International Society for Zinc Biology

Service

Manuscript Reviewer (abridged)

- Advances in Nutrition

- Biological Trace Element Research

- British Journal of Nutrition

- European Journal of Haematology

- Journal of Cellular Physiology

- Journal of Nutrition

- Nutrients

- PLOS ONE

Abstract Reviewer

- Nutrition 2018

- Experimental Biology, 2017

- Experimental Biology, 2018

Representative

- Post-doctoral Representative: ASN Nutritional Sciences Council, 2016-2018

- Graduate Student Representative: College of Health and Human Development

Academic Integrity Committee, 2012-2014

Hennigar, S.R. 11

- Student Representative: ASN Vitamins & Minerals Research Interest Section,

2011-2012

Chair

- Micronutrient Bioavailability, Antioxidant Function, Zinc, and Selenium Mini-

Symposium, Experimental Biology Meeting, 2017

- Zinc & Selenium Mini-Symposium, Experimental Biology Meeting, 2013

Judge

- Nutritional Sciences Council Graduate Student Research Competition, Nutrition

2018

- Postdoctoral Research Award Competition, Nutrition 2018

- Nutritional Sciences Council Graduate Student Research Competition,

Experimental Biology Meeting, 2017

- Emerging Leaders Poster Competition, Experimental Biology Meeting, 2017

- Pennsylvania Junior Academy of Sciences State Competition, 2012

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HONORS & AWARDS

Recognition

Dannon Nutrition Leadership Institute, 2017

Post-Doctoral Research Award Finalist, Experimental Biology, 2016

Emerging Leaders in Nutritional Sciences Poster Competition (5th place), Experimental

Biology, 2016

Selected Student Presentation, International Society for Zinc Biology Meeting, 2014

American Physiology Society Endocrinology & Metabolism Campbell Award,

Experimental Biology, 2014

International Society for Research in Human Milk and Lactation Young Investigator

Award, Experimental Biology, 2014

Vitamins & Minerals Research Interest Section Poster Competition (2nd place),

Experimental Biology, 2014

Nominated for Penn State Harold F. Martin Graduate Assistant Outstanding Teaching

Award, 2013

University of New Hampshire Graduate Student Teaching Award, 2010

University of New Hampshire Undergraduate Research Award, 2008

America East Student Athlete Honor Roll, 2005-2008

Assistantships & fellowships

Oak Ridge Institute for Science and Education Fellowship, 2014-2017

Kligman Graduate Fellowship Endowment, 2014

Ruth L. Pike Scholarship in Basic Nutrition Science, 2013

Penn State College of Health and Human Development Professional Development

Endowment, 2013

Penn State Research Assistantship, 2010-2014

University of New Hampshire Teaching Assistantship, 2008-2010

Hennigar, S.R. 12

University of New Hampshire College of Life Sciences and Agriculture Summer

Research Fellowship, 2009

University of New Hampshire Undergraduate Research Opportunity Project Award, 2007

Travel awards

Department of Nutritional Sciences Travel Award, Experimental Biology Meeting

(Boston, MA), 2013

Department of Nutritional Sciences Travel Award, Experimental Biology Meeting (San

Diego, CA), 2012

Department of Molecular, Cellular, and Biomedical Sciences Travel Award,

Experimental Biology meeting (Anaheim, CA), 2010

University of New Hampshire Graduate School Travel Award, Experimental Biology

meeting (Anaheim, CA), 2010

Department of Molecular, Cellular, and Biomedical Sciences Travel Award, Society of

Environmental Toxicology & Chemistry meeting (Durham, NH), 2009

Department of Molecular, Cellular, and Biomedical Sciences Travel Award,

Experimental Biology meeting (San Diego, CA), 2008

Undergraduate Research Opportunity Project Research Presentation Grant, Experimental

Biology meeting (San Diego, CA), 2008

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Updated August 9, 2018