sources of bovine sarcocystosis at farm : an innovative

Sources of bovine sarcocystosis at farm : an innovative approach MATERIALS AND METHODS A case-control study has been conducted on 60 farms in six different areas of France. The first step consisted in a qualitative investigation based on a detailed questionnaire, to describe precisely the breeding management system in the selected farms. The second line of action consisted in taking environmental samples (pasture grass, water and soil) to detect and identify environmental cattle Sarcocystis species in different zones in farms at risk (positive for BEM) and control farms. For each sample, a Multiplex PCR protocol targeting the 18s RNA gene and mitochondrial COI gene was applied to determine the different Sarcocystis species. • Sarcocystis species differences found in the environmental samples were not correlated to the status of farms. However, the presence of Sarcocystis in pasture grass (irrespective to the species) was found to be a significant risk factor. • Accordingly to scientific literature, S. cruzi was frequent and S. hirsuta very rare. S. hominis was quite rare, and found only on grass samples. PERSPECTIVES This exploratory study is to be considered a first step and it would be interesting to complete the results with additional investigations. Acknowledgments : All farmers who contributed to the survey, Interbev and the members of Interbev regional commitees. Support and funding: 1 Institut de l’Elevage, Service Qualité des Viandes, Villers-Bocage, France, 2 Institut de l’Elevage, Service Data’Stat, Lyon, France, 3 Università degli studi di Torino, Dipartimento di Scienze Veterinarie, Torino, Italia. Contact : marie [email protected] M. Drouet 1 , MN. Fouilloux 2 , S. Rubiola 3 , F. Chiesa 3 INTRODUCTION Sarcocystosis is a parasitic disease caused by protozoan coccidian parasites. Several experimental evidences have suggested that Sarcocystis species are responsible for bovine eosinophilic myositis (BEM) lesions. A statistical analysis using stratified multivariate logistic models has been carried out to identify risk factors which significantly (at a 5% threshold) increase the probability for a farm to be a case (ie positive for BEM). Pasture surface standard deviation Probability Probability Flood pastures effect Figures 1 & 2 : two significant risk factors which increase the probability for a farm to be a case : pasture surface and presence of flood pastures RESULTS • Several significant risk factors have been brought to light, in particular linked to grazing/breeding activities : increase of pasture surface, presence of flood pastures, decrease of grazing period, increase of mean number of calving per cow. Presence of Flood pastures : Quantitative factor : pasture surface Qualitative factor : flood pasture No Yes

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Sources of bovine

sarcocystosis at farm :

an innovative approach

MATERIALS AND METHODSA case-control study has been conducted on 60 farms in six different areas of France.The first step consisted in a qualitative investigation based on a detailedquestionnaire, to describe precisely the breeding management system in the selectedfarms. The second line of action consisted in taking environmental samples (pasturegrass, water and soil) to detect and identify environmental cattle Sarcocystis species indifferent zones in farms at risk (positive for BEM) and control farms. For each sample,a Multiplex PCR protocol targeting the 18s RNA gene and mitochondrial COI gene wasapplied to determine the different Sarcocystis species.

• Sarcocystis species differences found in the environmental samples were not correlated to the status of farms.However, the presence of Sarcocystis in pasture grass (irrespective to the species) was found to be a significantrisk factor.

• Accordingly to scientific literature, S. cruzi was frequent and S. hirsuta very rare. S. hominis was quite rare, andfound only on grass samples.

PERSPECTIVESThis exploratory study is to be considered a first step and it would be interesting to complete the results withadditional investigations.

Acknowledgments: All farmers who contributed to the survey, Interbev and the members of Interbev regional commitees.

Support and funding:1 Institut de l’Elevage, Service Qualité des Viandes, Villers-Bocage, France,2 Institut de l’Elevage, Service Data’Stat, Lyon, France,3 Università degli studi di Torino, Dipartimento di Scienze Veterinarie, Torino, Italia.Contact : [email protected]

M. Drouet1, MN. Fouilloux2, S. Rubiola3, F. Chiesa3

INTRODUCTIONSarcocystosis is a parasitic disease caused by protozoan coccidian parasites. Several experimental evidences havesuggested that Sarcocystis species are responsible for bovine eosinophilic myositis (BEM) lesions.

A statistical analysis using stratified multivariate logistic models has been carried out to identify risk factors which

significantly (at a 5% threshold) increase the probability for a farm to be a case (ie positive for BEM).

Pasture surface standard deviation

Pro

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ility

Pro

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ility

Flood pastures effect

Figures 1 & 2 : two significant risk factors which increase the probability for a farm to be a case : pasture surface and presence of flood pastures

RESULTS• Several significant risk factors have been brought to light, in particular linked to grazing/breeding activities :